Staphylococcus aureus

Sat, 01/25/2020 - 03:24
Rats are a reservoir of human- and livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats were genotyped and profiled for antibiotic resistances and human-specific...
Fri, 01/24/2020 - 03:35
by Sarah Ben Maamar, Adam J. Glawe, Taylor K. Brown, Nancy Hellgeth, Jinglin Hu, Ji-Ping Wang, Curtis Huttenhower, Erica M. Hartmann The decades-long global trend of urbanization has led to a population that spends increasing amounts of time indoors. Exposure to microbes in buildings, and specifically in dust, is thus also increasing, and has been linked to various health outcomes and to...
Fri, 01/24/2020 - 03:33
Author(s): Gülden Göksen, Maria José Fabra, H. Ibrahim Ekiz, Amparo López-RubioAbstractThe aim of this work was to develop novel active edible electrospun coatings with antimicrobial properties through the encapsulation of essential oils (EOs) from two widely used spices (Laurus nobilis and Rosmarinus officinalis) for inactivating or reducing spoilage and pathogenic microorganisms. Firstly, EOs...
Fri, 01/24/2020 - 03:24
Increased prevalence of antibiotic resistance in skin and soft tissue infections is a concerning public health challenge currently facing medical science. A combinatory, broad spectrum biocidal antiseptic has been developed (“ASP”) as a topically applied solution to potential resistant and polymicrobial infected wounds that may be encountered in this context. The ASP-105 designate was evaluated...
Fri, 01/24/2020 - 03:24
Staphylococcus aureus is an important pathogen that is associated with nosocomial infections, as well as food poisoning. This bacterium is resistant to antimicrobial agents and can survive in a wide range of environmental conditions. The aim of this study was to measure the uptake and release of amino acids by S. aureus at mid-exponential and stationary phases of growth following exposure to a...
Thu, 01/23/2020 - 03:31
Author(s): Hao Cheng, Muhammad Aslam Khan, Zhenfeng Xie, Shengnan Tao, Yunxing Li, Li LiangAbstractThe combination of different antimicrobial agents might produce synergistic effects and has gained increasing interest. Interfacial engineering of emulsion systems has been developed to co-encapsulate and protect bioactive components with different solubility. In this study, peppermint oil and...
Thu, 01/23/2020 - 03:31
Author(s): Liping Zhou, Tingwei Xu, Jicheng Yan, Xin Li, Yuqing Xie, Hao ChenAbstractThe konjac glucomannan (hereafter referred to simply as konjac) hydrogel has favorable gel-forming ability and swelling capacity, but the insufficient flexibility and poor water retention have limited its utilization. In this study, composite gels based on konjac and fish gelatin (FG) were prepared by alkali...
Thu, 01/23/2020 - 03:27
Aims This study was conducted to early detect the negative culture bacterial pathogens causing subclinical mastitis for the fast diagnosis of the disease and the reduction of some milk transmitted pathogenic bacteria to human consumers. Methods and Results A total of 171 positive California mastitis test (CMT) milk samples collected from asymptomatic dairy cows in Sharkia Governorate, Egypt were...
Thu, 01/23/2020 - 03:25
Phage lytic proteins are promising antimicrobials that could complement conventional antibiotics and help to combat multi-drug resistant bacteria that cause important human and animal infections. Here, we report the characterization of endolysin LysRODI (encoded by staphylophage phiIPLA-RODI) and its application as a prophylactic mastitis treatment. The main properties of LysRODI were compared...
Thu, 01/23/2020 - 03:24
The aim of this study was the assessment of the effect of time exposure, temperature, distance, and organic contaminants on radiant catalytic ionization (RCI) microbicidal effectiveness. The number of all examined bacteria decreased together with time exposure of RCI. The lowest recovery was obtained, both from the rubber surface (6.36 log CFU × cm–2) and steel (6.04 log CFU × cm–2) in the case...
Wed, 01/22/2020 - 03:36
ABSTRACT Machine learning has proven to be a powerful method to predict antimicrobial resistance (AMR) without using prior knowledge for selected bacterial species-antimicrobial combinations. To date, only species-specific machine learning models have been developed, and to the best of our knowledge, the inclusion of information from multiple species has not been attempted. The aim of this study...
Wed, 01/22/2020 - 03:35
Staphylococcus aureus infection poses a serious threat to public health, and antibiotic resistance has complicated the clinical treatment and limited the solutions available to solve this problem. Cold atmospheric plasma (CAP) is a promising strategy for microorganism inactivation. However, the mechanisms of microbial inactivation or resistance remain unclear. In this study, we treated S. aureus...
Wed, 01/22/2020 - 03:33
Author(s): Christina Charmpi, David Van der Veken, Emiel Van Reckem, Luc De Vuyst, Frédéric LeroyAbstractAcidification level and temperature modulate the beneficial consortia of lactic acid bacteria (LAB) and coagulase-negative staphylococci (CNS) during meat fermentation. Less is known about the impact of other factors, such as raw meat quality and salting. These could for instance affect the...
Wed, 01/22/2020 - 03:26
Background Many foods contain proteins and polyphenols, but there is a poor understanding of the nature of the inhibitory effect of protein on the biologic activity of polyphenols. The inhibitory mechanism of the food protein lactoferrin on the antibacterial activity of oligomeric ellagitannin oenothein B (OeB) was investigated using fluorescence quenching, isothermal titration calorimetry (ITC...
Wed, 01/22/2020 - 03:24
Staphylococcus aureus is one of the main pathogens found in cheeses produced with raw milk, including Minas artisanal cheese from Brazil. However, information about S. aureus isolated from artisanal cheeses and its sources of production in small-scale dairies is very limited. We aimed to characterize the virulence factors of S. aureus isolated from raw milk, endogenous starter culture, Minas...
Tue, 01/21/2020 - 03:29
LWT
Author(s): Haiying Cui, Chenghui Zhang, Changzhu Li, Lin LinAbstractIn recent years, studies have found that in addition to nosocomial infections, methicillin-resistant Staphylococcus aureus (MRSA) can even cause community-associated infections, and MRSA spreads through food and animal that produce products in the community. In addition, MRSA can colonize the surface of food and food container...
Sun, 01/19/2020 - 03:25
BACKGROUND In this study, we evaluated the combined effect of cinnamon essential oil (CEO) microemulsions and a temperature buffering package using phase change material (PCM) microcapsules on the physicochemical property, lipid oxidation and bacterial load of traditional Chinese pork balls (Shi Zi Tou) during temperature fluctuation storage for 9 days. RESULTS Transmission electron microscope...
Sun, 01/19/2020 - 03:23
Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used to treat bacterial infections since antiquity due to its known antimicrobial properties. Here, we aimed to evaluate the in vitro activity of colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total of 270 strains (Acinetobacter baumannii (n = 45...
Sat, 01/18/2020 - 03:24
Staphylococcus aureus strains resistant to the last line antibiotic, vancomycin, have been of clinical concern. These include heterogeneous vancomycin-intermediate S. aureus (hVISA) and VISA. The hVISA phenotype cannot be detected by routine laboratory methods. Characterization of hVISA/VISA by new technologies is necessary to differentiate them rapidly from the vancomycin-susceptible isolates (...
Thu, 01/16/2020 - 03:29
Author(s): Abdelrahim H.A. Hassan, Catherine N. CutterAbstractA novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods. Initially, CAFs were developed by incorporating thymol (T), nisin (N) and/or lauric arginate (LAE) into the pullulan layer and layering it on top of...
Thu, 01/16/2020 - 03:23
Staphylococcus aureus is one of the most important pathogens causing mastitis in cattle, and it is responsible for economic losses in dairy herds worldwide. The PCR amplification of the 16S-23S rRNA intergenic spacer (ribosomal spacer PCR, RS-PCR) allows a rapid classification of the strains in genotypes and genotypic clusters (CL), which are characterized by different epidemiological and...
Thu, 01/16/2020 - 03:23
It was previously thought that the Panton-Valentine leukocidin (PVL) toxin of Staphylococcus aureus (S. aureus) was not the main cause of cow mastitis. However, in recent years, detection of the gene encoding PVL has been increasing in dairy cow mastitis, which implies that PVL may be related to bovine mastitis. Therefore, we wanted to search for drugs inhibiting PVL or PVL-induced apoptosis. In...
Wed, 01/15/2020 - 03:32
Author(s): Shicun Jin, Kuang Li, Qiang Gao, Wei Zhang, Hui Chen, Jianzhang Li, Sheldon Q. ShiAbstractThe development of high-performance and sustainable bio-based adhesives has become a significant strategy for solving concerns regarding environmental pollution, resource utilization, and public health in the material engineering and production industries. In this work, hyperbranched silicone was...
Wed, 01/15/2020 - 03:27
The present study investigated in vitro antimicrobial activity of Eryngium caeruleum essential oil (EEO) against some of the fish spoilage and fish‐borne pathogenic bacteria. Moreover, its effects on specific spoilage microorganisms, inoculated Listeria monocytogenes, and its sensory changes in minced fish were evaluated. The results showed that EEO had a significant in vitro antimicrobial effect...
Tue, 01/14/2020 - 03:23
Dairy product safety is a global public health issue that demands new approaches and technologies to control foodborne pathogenic microorganisms. Natural antimicrobial agents such as nisin can be added to control the growth of pathogens of concern in dairy foods, namely Listeria monocytogenes and Staphylococcus aureus. However, several factors affect the antimicrobial efficacy of nisin when...
Sun, 01/12/2020 - 03:32
Author(s): Chujun Ou, Daiqi Shang, Jingxian Yang, Bo Chen, Jiang Chang, Fangning Jin, Chunlei ShiAbstractAntimicrobial resistance has gradually become a serious problem threatening public health and food safety throughout the world. Biofilm is one of the important factors affecting the antimicrobial resistance of bacteria. Staphylococcus aureus usually has strong biofilm formation ability, and it...
Fri, 01/10/2020 - 03:31
Author(s): Vinaya Kizhakkepatt Kizhakkekalam, Kajal Chakraborty, Minju JoyABSTRACTThree homologous oxygenated elansolid-type of polyketide spanned macrolides were isolated from a heterotrophic marine bacterium, Bacillus amyloliquefaciens MTCC 12716 associated with an intertidal red alga Hypnea valentiae. We have sequenced the complete genome of the bacterium and analyzed all detectable natural...
Fri, 01/10/2020 - 03:30
Author(s): Dongyun Jung, Joseph E. RubinAbstractThe role of plant-based foods in the epidemiology of antimicrobial resistance has been inadequately studied. In this investigation, resistant organisms from vegetables, fruits and spices imported into Canada were identified and characterized. A total of 143 products imported from primarily Asian and African countries were purchased from...
Fri, 01/10/2020 - 03:30
Author(s): Olouwafemi Mistourath Mama, Liliana Morales, Laura Ruiz-Ripa, Myriam Zarazaga, Carmen TorresAbstractMethicillin-resistant Staphylococcus aureus (MRSA) CC398 is a livestock-associated (LA) lineage, mainly detected in swine. Its dissemination via the food-chain could be a food-safety issue. This work aimed to study the diversity of S. aureus lineages in pork-products, to determine the...
Wed, 01/08/2020 - 03:34
LWT
Author(s): Qian Hua, Pei Gao, Yanshun Xu, Wenshui Xia, Yingying Sun, Qixing JiangAbstractThe effects of commercial starter cultures on the quality characteristics of fermented fish-chili paste were investigated. Five batches of fish-chili paste were prepared with different starter cultures, namely, SBM-52 (Staphylococcus xylosus, Staphylococcus carnosus, Pediococcus pentosaceus and Pediococcus...
Wed, 01/08/2020 - 03:27
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has been reported to colonize and cause infections in animals as well as in humans. LA-MRSA isolates have only recently been identified in patients admitted to Kuwait hospitals. This study was conducted to characterize LA-MRSA isolates obtained from patients admitted to Kuwait hospitals. A total of 202 (7.1%) of 2,823 MRSA...
Tue, 01/07/2020 - 03:30
The current study demonstrated that SEU promoted the proinflammatory activity of macrophage via upregulation of AIF‐1 expression. The expression of AIF‐1 was inferred by RNAi and the interference abolished the increase of cell proliferation and inflammatory cytokine TNFα, IL6 secretion. Abstract Staphylococcal enterotoxins (SEs) are a wide array of virulence factors produced by Staphylococcus...
Mon, 01/06/2020 - 03:49
Staphylococcus aureus (S. aureus) is an important foodborne pathogen that causes food poisoning and severe infections. Herein, we design a novel and sensitive colorimetric immunoassay for the detection of S. aureus based on the formation of a magnetic bead-based sandwich complex and signal amplification via an enzyme mimic. Magnetic nanoparticles decorated with carbon dots offer rapid enrichment...
Sun, 01/05/2020 - 03:31
LWT
Author(s): Nam Hee Kim, Hye Won Kim, Hyeree Moon, Min Suk RheeAbstractThis study investigated the synergistic effects of sodium chloride and essential oils (EOs) on three representative halotolerant species of foodborne pathogens, Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus. When bacterial cells were treated with carvacrol or thymol alone (0.5–2.0 mM), NaCl alone (...
Sat, 01/04/2020 - 03:36
Author(s): Xiaoling Dai, Xin Huang, Zhuoxing Zhang, Ruidong Zhang, Xueying Cao, Chao Zhang, Kaiqiang Wang, Qian RenAbstractInnate immunity is the primary defense of crustaceans against pathogens. Crustins, as antimicrobial peptides, are important to crustacean innate immunity. In this study, two kinds of Gly-rich crustin genes were cloned from Macrobrachium nipponense and were referred to as Mn-...
Sat, 01/04/2020 - 03:36
Author(s): Chao Zhang, Xueying Cao, Kaiqiang Wang, Xiaoling Dai, Ruidong Zhang, Zhuoxing Zhang, Xin Huang, Qian RenAbstractTranscription factor activator protein 1 (AP1) plays an irreplaceable role in the response to a variety of external stimulants, such as cellar stress, bacterial and viral infections, and inflammatory cytokines. In this study, we identified a novel AP1 gene from Macrobrachium...
Sat, 01/04/2020 - 03:36
Author(s): Lei Liu, Yunfei DangAbstractMannose-binding lectin (MBL) is a crucial pattern recognition receptor in the host innate immune system. Previously, we reported the biological function of Ctenopharyngodon idella MBL (CiMBL) in initiating the lectin pathway of the complement system. In the present study, we further explored its biological function including the agglutinating ability,...
Fri, 01/03/2020 - 04:13
Here, we report the genome sequence of the megaplasmid-bearing Staphylococcus sciuri strain B9-58B, isolated from retail pork. This strain contains a 2,761,440-bp chromosome and a 162,858-bp megaplasmid. The genome contains putative genes involved in virulence, the stress response, and antimicrobial agent and heavy metal resistance.
Fri, 01/03/2020 - 03:48
Staphylococcus hyicus, considered as a leading pathogen of exudative epidermitis, is a serious threat to humans and animals. The emergency of bacterial resistance to antibiotics, especially in human and animal health fields, leads to an urgent need of exploration of new antimicrobial agents. In this study, NZX, a plectasin-derived peptide, was firstly expressed in Pichia pastoris X-33 and was...
Fri, 01/03/2020 - 03:48
Streptococcus mutans is a common principal causative agent of dental caries. In this communication, we describe that the antibodies raised against purified dextransucrase effectively inhibited the growth of S. mutans. The purified enzyme showed 58-fold enrichment, 17.5% yield and a specific activity of 3.96 units/mg protein. Purified IgG fraction of the antibody showed significant affinity with...
Tue, 12/31/2019 - 03:44
Drug-resistant bacteria threaten the health of people world-wide and cause high costs to their health systems. According to Scientific American, the number of regrettable fatalities due to the bacteria that are resistant to conventional antibiotics will sum up to 300 million until 2050 if the problem is not tackled immediately. Photodynamic Inactivation (PDI) has proven effective against...
Mon, 12/30/2019 - 03:33
Author(s): Marilinda Lorenzini, Giacomo ZapparoliAbstractThe bacterial community in the surface of withered grapes, which are partially dehydrated in the post-harvest period to produce Italian passito wine, has been seldom investigated. Fifty epiphytic bacterial strains isolated from withered berries were identified and characterized. Genera such as Bacillus, Brevibacillus, Curtobacterium,...
Mon, 12/30/2019 - 03:28
The morphological structure of potato starch‐based biodegradable (PSBB) films with virgin coconut oil (VCO). Increasing VCO concentrations caused a rise in the light transmittance of the films from 2.13 to 4.79 mm−1 and a decrease in water vapour transmittance from 6.77 to 2.12 (10−5 GPa−1 h−1 m−1). Scanning electron microscopy showed that the surface of the film became smoother as VCO...
Sat, 12/28/2019 - 03:24
Staphylococcus aureus is a major human pathogen and an important cause of livestock infections. More than 20 staphylococcal enterotoxins with emetic activity can be produced by specific strains responsible for staphylococcal food poisoning, one of the most common food-borne diseases. Whole genome sequencing provides a comprehensive view of the genome structure and gene content that have largely...
Fri, 12/27/2019 - 03:33
LWT
Author(s): Belal J. Muhialdin, Hana Kadum, Mohammad Zarei, Anis Shobirin Meor HussinAbstractIn this study dragon fruit juice was subjected to lacto-fermentation for 48 h at 37 °C using Lactobacillus plantarum FBS05. The antibacterial and antioxidant activity were evaluated using 96 microtiter plate, DPPH and FRAP assays. The bioactive metabolites were identified using 1H-NMR analysis with...
Thu, 12/26/2019 - 03:33
Author(s): Nemanja Mirkovic, Jelena Kulas, Zorana Miloradovic, Marija Miljkovic, Dina Tucovic, Jelena Miocionovic, Branko Jovcic, Ivana Mirkov, Milan KojicAbstractIn last two decades, there has been a strong trend in the application of lactic acid bacteria as adjunctive cultures to control growth of spoilage and pathogenic bacteria in food. One of the most important properties that contribute to...
Wed, 12/25/2019 - 03:30
Author(s): Maryam Azarifar, Babak Ghanbarzadeh, Mahmoud Sowti Khiabani, Afshin Akhondzadeh Basti, Ali AbdulkhaniAbstractThe effects of gelatin-carboxymethyl cellulose (Gel-CMC) based films containing chitin nano fiber (CHNF) and Trachyspermum ammi essential oil (Ajowan), on the shelf life extension of the raw beef at refrigerated temperature (4 °C) over a 12-day period were evaluated. Ajowan...
Tue, 12/24/2019 - 03:35
by Warren Chanda, Mespa Manyepa, Ephraim Chikwanda, Victor Daka, Justin Chileshe, Mathias Tembo, Joseph Kasongo, Allen Chipipa, Ray Handema, John A. Mulemena Periodic monitoring of antibiotic susceptibility patterns in clinical settings is vital to ascertain the potency as well as re-establishing empirical therapy. This retrospective study aimed to evaluate the antibiotic susceptibility patterns...
Tue, 12/24/2019 - 03:34
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) of clonal complex (CC) 398 has become a rising issue for public health. While it is known that >80% of pig farmers are colonized with LA-MRSA, only a few studies have assessed the situation for humans with occasional livestock contact. Recently it was shown that over 75% of scientific fieldworkers visiting pigsties were...

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