Staphylococcus aureus

Tue, 12/24/2019 - 03:34
The intrinsic resistance of Pseudomonas aeruginosa to many antibiotics limits treatment options for pseudomonal infections. P. aeruginosa's outer membrane is highly impermeable and decreases antibiotic entry into the cell. We used an unbiased, high-throughput approach to examine mechanisms underlying outer membrane-mediated antibiotic exclusion. Insertion Sequencing (INSeq) identified genes that...
Sun, 12/22/2019 - 03:32
Author(s): Yue Wang, Xue-Peng Wang, Bin Zhang, Zhi-Min Li, Li-Guo Yang, Xin-Cang Li, Hongyu MaAbstractLysin motif (LysM)-containing proteins function as pattern-recognition receptors in plants to recognize different N-acetylglucosamine-containing ligands, thereby triggering specific defense responses against pathogens. However, the biological functions of these proteins in animals remain unclear...
Sat, 12/21/2019 - 04:07
Community-onset bloodstream infections (CO-BSI) are major causes of severe febrile illness and death worldwide. In light of new data and the growing problem of antimicrobial resistance (AMR) among pathogens causing BSI, we undertook a systematic review of hospital-based studies of CO-BSI among patients hospitalized with fever. Without restriction to language or country, we searched PubMed, Web of...
Sat, 12/21/2019 - 04:07
It is often difficult to cure endocarditis, osteomyelitis, and device-associated infections caused by Gram-positive pathogens, despite therapy with clinically appropriate antibiotics. This may be due to antibiotic tolerance or resistance development. Acyldepsipeptides (ADEPs) are a class of bactericidal compounds active against a variety of clinically important Gram-positive bacteria, including...
Sat, 12/21/2019 - 03:58
Author(s): Ana Carolina Justino de Araújo, Priscilla Ramos Freitas, Cristina Rodrigues dos Santos Barbosa, Débora Feitosa Muniz, Janaína Esmeraldo Rocha, Ana Cristina Albuquerque da Silva, Cícera Datiane de Morais Oliveira-Tintino, Jaime Ribeiro-Filho, Luiz Everson da Silva, Camila Confortin, Wanderlei do Amaral, Cícero Deschamps, José Maria Barbosa-Filho, Natanael Teles Ramos de Lima, Saulo...
Sat, 12/21/2019 - 03:42
Background:Resistance to antibiotics is steadily increasing. Ceftaroline has a broad spectrum of activity against clinically relevant gram-positive strains including methicillin-resistant Staphylococcus aureus.Objectives:This systematic review was conducted to evaluate whether ceftaroline is effective and safe, leading to a lower rate of treatment failures than comparators.Material and methods:...
Sat, 12/21/2019 - 03:42
In the present study, Veronica multifida leaf extract and zinc acetate dihydrate were utilized to synthesize zinc oxide (ZnO) nanoparticles (NPs) eco-friendly and cost-effectively under different physical conditions. Soxhlet extractor was used for the preparation of aqueous plant extract. UV-Vis (ultraviolet–visible) spectrophotometer, X-ray diffraction (XRD), Fourier transform infrared...
Fri, 12/20/2019 - 03:36
Author(s): Mathieu Weil, Fabienne Remize, Noel Durand, Pascaline Alter, Mathilde Hoarau, Jean Christophe MeileAbstractThe management of microbial contamination is an important issue in spice trade. For common domesticated black pepper (Piper spp.), the control relies mainly on post-process decontamination. The aim of the present study was to examine microbial contamination of wild pepper (Piper...
Thu, 12/19/2019 - 03:36
Author(s): Francesca Patrignani, Lorenzo Siroli, Giacomo Braschi, Rosalba LanciottiAbstractThe present research was aimed to investigate the potentialities of ultra high pressure homogenization (UHPH) to produce stable natural antimicrobial based nanoemulsions. Initially, the nanoemulsions were characterized for their size, stability over time and antimicrobial properties against several...
Thu, 12/19/2019 - 03:33
LWT
Author(s): Naser Karimi, Ainaz Alizadeh, Hadi Almasi, Shahram HanifianAbstractThere is growing interest in bioactive packaging systems due to their potential for extending the shelf life of food products. In this regard, the aim of this study was to prepare cellulose nanofiber (CNF) and polydextrose incorporated whey protein isolate (WPI) based probiotic nanocomposite film containing...
Thu, 12/19/2019 - 03:30
The aim of this work was to collect relevant input data for integration into Monte‐Carlo simulation using 10,000 iterations to obtain quantitative estimates of exposure and associated risk to Staphylococcus aureus and Salmonella. Higher prevalence rates of S. aureus (54.9 vs. 28.3%) and Salmonella (12.9 vs. 11.1%) were observed for raw milk distributed via collection milk collection centers (MCC...
Thu, 12/19/2019 - 03:30
The simultaneous effects of microwave processing variables affecting the microbial quality and preservation of milk bioactive proteins were evaluated. Response surface methodology was used to investigate the individual and combined effects of ramp time (2.9–5.5 min), holding time (6.6–23.4 s), and final temperature (60–80°C) on the inactivation of two surrogates (Staphylococcus aureus and...
Thu, 12/19/2019 - 03:26
Staphylococcus aureus (S. aureus) associated infections are one of the major threats to public health. The aim of the present study was to determine the antibiotic resistance pattern as well as the genetic characterization of methicillin and vancomycin resistant S. aureus (VRSA) isolated from a tertiary care hospital in Lahore. The S. aureus were isolated from different clinical samples,...
Wed, 12/18/2019 - 04:04
Author(s): Mahmoud Abdel-Hamid, Ehab Romeih, Paola Saporito, Ali Osman, Ramona Valentina Mateiu, Biljana Mojsoska, Håvard JenssenAbstractPseudomonas aeruginosa PAO1 and Methicillin-Resistant Staphylococcus aureus (MRSA) are amongst the most virulent pathogens, causing chronic and life-threatening human infections. Thus, novel natural compounds able to inhibit these pathogens, reduce and/or...
Wed, 12/18/2019 - 03:43
Antimicrobial resistance represents one of the greatest challenges of the twenty-first century, and it is globally recognized that addressing this problem requires a concerted One Health approach involving humans, animals, and the environment. Methicillin-resistant Staphylococcus aureus (MRSA) currently represents a global burden; it is resistant to almost all beta-lactams and some MRSA strains...
Wed, 12/18/2019 - 03:43
The present study was conducted from July to August 2018 on milk samples taken at dairy farms in the Northern Province and Kigali District of Rwanda in order to identify Staphylococcus spp. associated with bovine intramammary infection. A total of 161 staphylococcal isolates originating from quarter milk samples of 112 crossbred dairy cattle were included in the study. Antimicrobial...
Sun, 12/15/2019 - 03:36
Author(s): Felix K.A. Kuebutornye, Zhiwen Wang, Yishan Lu, Emmanuel Delwin Abarike, Michael Essien Sakyi, Yuan Li, Cai Xia Xie, Vivian HlordziAbstractSkin and intestinal mucosa lymphoid tissues are known to be the fish's first line of defence since they serve as the first point of contact for pathogens. Only few studies have investigated the influence of host-associated Bacillus on mucosal...
Sat, 12/14/2019 - 03:38
Author(s): Yingying Zhao, Dandan Xia, Panpan Ma, Xiaofeng Gao, Wenyi Kang, Jinfeng WeiAbstractHuman health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from...
Sat, 12/14/2019 - 03:35
Author(s): Alonzo A. Gabriel, Mikaella Marie C. David, Maxine Sebastianne C. Elpa, Juan Carlos D. MichelenaAbstractThis study determined the efficacy of UV-C as a decontamination process against some foodborne bacteria in dried whole black peppercorns. Artificially-inoculated Salmonella enterica, Escherichia coli O157:H7, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus...
Fri, 12/13/2019 - 03:27
Foods may potentially serve as vehicles for the transmission of antimicrobial-resistant variants of Staphylococcus aureus that are important in a human clinical context. Further, retail food products can be a cause of staphylococcal food poisoning. For these reasons and to account for source attribution and risk assessment, detailed information on the population structure, resistance, and...
Thu, 12/12/2019 - 03:28
This study investigated the antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) isolated from cases of subclinical bovine mastitis in China, as well as resistance mechanisms and virulence genes encoding adhesins and toxins. We determined antimicrobial susceptibility using the disk diffusion method, and analyzed resistance, adhesin, and toxin genes using PCR. We...
Thu, 12/12/2019 - 03:28
Mastitis in ruminants is an important disease with major effects on both the economy and animal welfare. It is caused by major pathogens such as Staphylococcus aureus and minor pathogens such as coagulase-negative staphylococci. The objective of this study was to identify and characterize staphylococci as a cause of sheep mastitis in Algeria. In this study, 123 milk samples were collected...
Sat, 12/07/2019 - 03:34
Author(s): Matthew Bankowski, Jason Blanton, Paul Fiorella, Jessy Motes, Nancy Pickens, Jeremy Racicot, Aaron Odegard, Patricia DuBose, Man-Kuang Chang, Charles AbaabaAbstractStaphylococcus epidermidis and a Gram negative bacillus (GNB) were isolated in blood cultures from a 68 year old male with cancer. The GNB was suspicious for Brucella spp., but was identified using 16S rDNA sequencing...
Sat, 12/07/2019 - 03:32
LWT
Author(s): Edinéia Bonin, Venício Macedo Carvalho, Vicente Diaz Avila, Naiara Caroline Aparecida dos Santos, Érica Beassi-Zanqueta, César Armando Contreras Lancheros, Isolde Terezinha Santos Previdelli, Tania Ueda Nakamura, Benício Alves de Abreu Filho, Ivanor Nunes do PradoAbstractDue to consumers' increasingly negative perceptions of synthetic preservatives, interest and research on plants and...
Sat, 12/07/2019 - 03:28
Aim The objective of this study was to investigate the biofilm inhibitory activity of Streptomyces‐derived actinomycin D against biofilm formation by Staphylococcus epidermidis. Methods and Results The microtiter plate method and microscopy were used to detect the biofilm formation of S. epidermidis. And an attempt was made to detect the effect of actinomycin D on important biofilm components,...
Fri, 12/06/2019 - 03:29
High prevalence of antimicrobial resistance (AMR) to drugs of critically/highly importance of human and veterinary medicine was observed among the recovered Escherichia coli, Salmonella spp., and Staphylococcus aureus, circulating over the different stages of poultry in India. Upward trend in AMR rates was observed in poultry‐retail shops than in poultry‐farms. Multi‐drug resistance was observed...
Wed, 12/04/2019 - 03:36
Author(s): Bing-Chang Lee, Chun-Wei Hung, Cheng-Yung Lin, Chen-Han Shih, Huai-Jen TsaiAbstractTo develop an alternative to conventional antibiotics used in the aquaculture and livestock industries, we employed Bacillus subtilis, considered a biosafe microorganism, to express the degradable antimicrobial peptide lactoferricin. An expression plasmid pP43-6LFBII-GFP, in which reporter GFP cDNA was...
Tue, 12/03/2019 - 03:58
Our review provides a comprehensive treatise on the progress in understanding teixobactin chemistry, structure−activity relationships, and mechanisms of antibacterial activity. Teixobactin represents an exciting starting point for the development of new antibiotics that can be used to combat multidrug‐resistant bacterial (“superbug”) infections. Abstract The discovery of antibiotics has led to...
Tue, 12/03/2019 - 03:50
The feeding trial was conducted to evaluate the potentials of Clostridium butyricum in the diet of tilapia. Fish (~14 g) were fed with basal diet supplemented with 0 (Control), 0.5 (C‐1), 1 (C‐2), 2 (C‐3), 4 (C‐4) and 8 (C‐5) g/kg commercial probiotic‐containing C. butyricum (1.5 × 108 CFU/g) for 8 weeks. The results showed that weight gain significantly increased, and feed conversion ratio...
Mon, 12/02/2019 - 03:36
Author(s): Gaoyang Wang, Yutong Lei, Ting Kang, Zhi Li, Hui Fei, Shun Yang, Peng Zhou, Chunsheng Wang, Zhengbing Lv, Mengmeng Huang, Xuewei XuAbstractC-type lectins are Ca2+-dependent carbohydrate-binding proteins containing one or more carbohydrate-recognition domains (CRDs). C-type lectins play crucial roles in innate immunity, including nonself-recognition and pathogen elimination. In the...
Mon, 12/02/2019 - 03:34
Author(s): Ivana Kubašová, Dzung B. Diep, Kirill V. Ovchinnikov, Andrea Lauková, Viola StrompfováAbstractOne of the relatively widespread properties of many enterococcal strains is the production of bacteriocins, which could be useful as natural food preservatives inhibiting pathogenic and spoilage microorganisms. However, knowledge of the distribution and spectrum of bacteriocin activity as well...
Mon, 12/02/2019 - 03:34
Author(s): Maged El-Ashker, Mayada Gwida, Stefan Monecke, Fatma El-Gohary, Ralf Ehricht, Mona Elsayed, Akinduti Paul, Mohamed El-Fateh, Sven MaurischatAbstractMethicillin-resistant S. aureus from cows with mastitis has received a growing interest worldwide. The present study aimed to provide a detailed description of the resistance and virulence traits of isolates from bovine mastitis samples.A...
Mon, 12/02/2019 - 03:32
LWT
Author(s): Andréia Saldanha de Lima, Darla Volcan Maia, Louise Haubert, Thaís Larré Oliveira, Ângela Maria Fiorentini, Cesar Valmor Rombaldi, Wladimir Padilha da SilvaAbstractAraçá (Psidium cattleianum Sabine) is a native fruit of South America, with high levels of bioactive compounds as well as antimicrobial potential. The aim of this study was to evaluate the antibacterial activity of the...
Sat, 11/30/2019 - 03:33
Author(s): Sophie Reissier, Isabelle Lazareth, Louise Adjiman, Carine Couzigou, Barbara Vidal, Assaf Mizrahi, Jean Claude Nguyen Van, Benoît Pilmis, Alban Le Monnier, Julie Lourtet-HascoëtAbstractObjectivesUnyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in...
Sat, 11/30/2019 - 03:28
The aim of the present study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. Seventeen samples of paired follicular fluids and uterine contents collected from post‐slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Further, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also...
Fri, 11/29/2019 - 03:29
Author(s): Brian Friker, Marina Morach, Sabrina Püntener, Nicole Cernela, Jule Horlbog, Roger StephanAbstractIn recent years, popularity of raw milk has increased in many industrialised countries.This study (i) enumerated total viable counts (TVC) and Escherichia coli counts, (ii) assessed prevalence of Staphylococcus (S.) aureus, Salmonella spp. and STEC, (iii) screened for methicillin resistant...
Fri, 11/29/2019 - 03:28
Author(s): Kuan-Hung Lu, Yi-Jyun Sheen, Tsui-Ping Huang, Shu-Hui Kao, Chun-Lung Cheng, Cheng-An Hwang, Shiowshuh Sheen, Lihan Huang, Lee-Yan SheenAbstractCooked rice with pork floss (CRPF) wrapped in dried seaweed is one of the most popular ready-to-eat (RTE) foods in many Asian countries, particularly in Taiwan. The products are susceptible to Staphylococcus aureus contamination and temperature...
Thu, 11/28/2019 - 04:14
by Felipe Betoni Saraiva, Ana Caroline Cavalcante de Araújo, Anna Érika Vieira de Araújo, José Procópio Moreno Senna Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium responsible for serious nosocomial and community-acquired infections worldwide. Since few antibiotics are effective for treating MRSA infections, the development of new therapies is of great...
Thu, 11/28/2019 - 04:03
Author(s): Cailin Liu, Chunguang Chen, Yafei Ye, Xiaogai Li, Jingjing Sun, Lijuan Xu, Liang MingAbstractTwo linezolid-resistant and teicoplanin-intermediate Staphylococcus epidermidis strains were isolated from blood cultures in China. The two S.epidermidis strains were methicillin-resistant and showed multidrug-resistance patterns, in addition, population analysis profiling/area under the curve...
Thu, 11/28/2019 - 03:49
Staphylococcus aureus is able to grow in the presence of high concentrations of NaCl but the exact genetic factors contributing to this are unknown. Using a high‐throughput TN‐seq approach, we identified gene 957 as an important factor for the salt stress resistance in S. aureus. A 957‐mutant was not only salt sensitive but also showed increased peptidoglycan crosslinking, altogether highlighting...
Wed, 11/27/2019 - 03:31
Methicillin‐resistant Staphylococcus aureus (MRSA), a versatile pathogen bearing multiple virulence determinants, is increasingly being detected in various food‐producing animals, including fish. In addition, it is a potential food poisoning agent. MRSA is not an inherent microbiota of fish; its presence is attributed to pre‐ or postharvest contamination through fish handlers, water, ice, and...
Mon, 11/25/2019 - 03:33
Author(s): Lele Shao, Yi Liu, Xiaojing Tian, Huicong Wang, Qianqian Yu, Xingmin Li, Ruitong DaiAbstractOhmic heating (OH), an alternative thermal processing technique, has various applications in food industry in recent years. To better understand the inactivation of microorganisms treated with OH, effects of OH with different voltage gradient (5 and 10 V/cm) and frequency (50 and 500 Hz) on the...
Sat, 11/23/2019 - 03:34
Author(s): Luca Settanni, Pietro Barbaccia, Adriana Bonanno, Marialetizia Ponte, Rosalia Di Gerlando, Elena Franciosi, Antonino Di Grigoli, Raimondo GaglioAbstractThe present work was carried out to evaluate the microbiological and physicochemical composition of salamis produced with the meat of beef, horse, wild boar and pork. Salami productions occurred under controlled laboratory conditions to...
Sat, 11/23/2019 - 03:25
Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) belonging to clonal complex 398 is recognized as an occupational hazard for workers employed in intensive animal husbandry, especially in th...
Fri, 11/22/2019 - 03:38
Here, we present data on the complete genome sequences of 11 Staphylococcus sp. isolates (three S. chromogenes isolates and one isolate each of S. saprophyticus, S. xylosus, S. hominis, S. agnetis, S. caprae, S. aureus, and S. warneri), obtained as part of a mastitis study of buffalo milk (from healthy animals and from those with subclinical mastitis) and milkers’ hands.
Fri, 11/22/2019 - 03:26
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) are a major concern for public health, and broiler farms are a potential source of MRSA isolates. In this study, a total of 56 MRSA isolates from 15 broiler farms from 4 different counties in Germany were characterised phenotypically and genotypically. Spa types, dru types, SCCmec types, and virulence genes as well as resistance genes...
Thu, 11/21/2019 - 03:37
by Elizeth Lopes, Teresa Conceição, Laurent Poirel, Hermínia de Lencastre, Marta Aires-de-Sousa Background In 2016, very high rates of methicillin-resistant Staphylococcus aureus (MRSA)-ST398 (99%) were found in Portuguese pig farms that used colistin, amoxicillin, and zinc oxide as feed additives. Since then, farms A and B banned the use of colistin, and farm C banned the use of both antibiotics...

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