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Vibrio

Thu, 05/16/2019 - 00:00
Sensitive detection of pathogenic bacteria is of great significance for food safety and public health. This study aimed to report a novel F0F1–ATPase aptasensor based on quantum dots (QDs) for quantitative detection of Vibrio parahaemolyticus. The fluorescent QDs were labeled onto the surface of chromatophores to act as an indicator. Aptamer target to V. parahaemolyticus was...
Wed, 05/15/2019 - 20:00
Bacterial pathogens maintain disulfide bonds for protein stability and functions that are required for pathogenesis. Vibrio parahaemolyticus is a Gram-negative pathogen that causes food-borne gastroenteritis and is also an important opportunistic pathogen of aquatic animals. Two genes encoding the disulfide bond formation protein A, DsbA, are predicted to be encoded in the V. parahaemolyticus...
Mon, 05/13/2019 - 17:00
S. Peter Howard, Leandro F. Estrozi, Quentin Bertrand, Carlos Contreras-Martel, Timothy Strozen, Viviana Job, Alexandre Martins, Daphna Fenel, Guy Schoehn, Andréa Dessen The type II secretion system (T2SS) is a cell envelope-spanning macromolecular complex that is prevalent in Gram-negative bacterial species. It serves as the predominant virulence mechanism of many bacteria including those of...
Mon, 05/13/2019 - 01:14
Available online 11 May 2019 Author(s): Débora Rodrigues Silveira, Janaina Viana da Rosa, Kauana Kaefer, Luiz Gustavo Bach, Amanda de Oliveira Barbosa, Cláudio Dias TimmAbstractThe present study aimed to investigate the presence of Vibrio vulnificus in fish captured at the Lagoa dos Patos estuary (RS, Brazil), to establish a correlation between bacterial biofilm formation and sublethal stress,...
Fri, 05/10/2019 - 05:04
Abstract Vibrio parahaemolyticus is the leading cause of illness associated with seafood consumption globally. In this study, we examined 136 V. parahaemolyticus isolates from Ningbo, China for antimicrobial resistance phenotypes, genotypes, virulence factor genes (tdh, trh, T3SS) and molecular typing. Carbenicillin resistance (100.0%) was the most prevalent in all isolates followed by...
Fri, 05/10/2019 - 05:04
Abstract Vibrio parahaemolyticus is the leading cause of illness associated with seafood consumption globally. In this study, we examined 136 V. parahaemolyticus isolates from Ningbo, China for antimicrobial resistance phenotypes, genotypes, virulence factor genes (tdh, trh, T3SS) and molecular typing. Carbenicillin resistance (100.0%) was the most prevalent in all isolates followed by...
Tue, 05/07/2019 - 20:00
Vibrio parahaemolyticus is a common pathogen which has become a major concern of seafood products. The bacteria in the viable but nonculturable (VBNC) state are unable to form colonies on growth media, but under appropriate conditions they can regain culturability. In this study, V. parahaemolyticus was induced into VBNC state at low temperature and oligotrophic condition, and was resuscitated to...
Mon, 05/06/2019 - 07:48
How motile bacteria recognize their environment and decide whether to stay or move is a fundamental issue in survival. Here we established the molecular mechanism underlying the intimate relationship between nutrient availability and flagellum‐driven motility in Vibrio vulnificus; dephosphorylated EIIAGlc of the PEP:carbohydrate phosphotransferase system sequesters the flagellar assembly protein...
Tue, 04/30/2019 - 20:00
Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus Maofeng Zhang1,2†, Xiyan Zhang1†, Liaowang Tong1†, Dexin Ou1, Yaping Wang1, Jumei Zhang2, Qingping Wu2* and Yingwang Ye1,2* 1School of Food Science and Engineering, Hefei University of Technology, Hefei, China 2State Key Laboratory of...
Mon, 04/29/2019 - 20:00
Surveillance and Genomics of Toxigenic Vibrio cholerae O1 From Fish, Phytoplankton and Water in Lake Victoria, Tanzania Yaovi M. Gildas Hounmanou1*, Pimlapas Leekitcharoenphon2, Rene S. Hendriksen2, Tamegnon V. Dougnon3, Robinson H. Mdegela4, John E. Olsen1 and Anders Dalsgaard1 1Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen,...
Mon, 04/29/2019 - 11:34
Vibrio cholerae, the facultative pathogen responsible for cholera disease, continues to pose a global health burden. Its persistence can be attributed to a flexible genetic toolkit that allows for adaptation to different environments with distinct carbon sources, including the six-carbon sugar alcohol mannitol. V. cholerae takes up mannitol through the transporter protein MtlA, whose production...
Wed, 04/24/2019 - 00:00
Vibrio cholerae is the bacterial pathogen responsible for the disease cholera, plaguing many developing nations and areas of poor sanitation, and causing about 2.9 million cases and ~95 000 deaths annually. It is transmitted through contaminated water and infects the host using two major virulence factors: the toxin-coregulated pilus (TCP) and cholera toxin (CT). TCP is important for colonization...
Tue, 04/23/2019 - 11:01
Vibrio parahaemolyticus is a human pathogen, and it is a major cause of severe gastroenteritis in coastal areas. OmpU is one of the major outer membrane porins of V. parahaemolyticus. Host-immunomodulatory effects of V. parahaemolyticus OmpU (VpOmpU) have not been elucidated yet. In this study, in an effort towards characterizing the effect of VpOmpU on innate immune responses of the host, we...
Mon, 04/22/2019 - 11:14
The bacterial flagellum is a sophisticated self-assembling nanomachine responsible for motility in many bacterial pathogens including Pseudomonas aeruginosa, Vibrio spp., and Salmonella enterica. The bacteria flagellum has been studied extensively in the model systems Escherichia coli and Salmonella Typhimurium, yet the range of variation in flagellar structure and assembly remains incompletely...
Mon, 04/22/2019 - 01:13
Available online 19 April 2019 Author(s): S. Sivakumar, T. Raja Swaminathan, R. Anandan, N. KalaimaniAbstractThe lack of shrimp cell lines and difficulty in establishing shrimp cell culture systems, with an appropriate medium is a major concern in the aquaculture sector. The present study attempts to address this issue by developing an in vitro cell culture system from various tissues (hemocytes...
Mon, 04/22/2019 - 00:00
Abstract Miang is a traditional fermented tea made from fermentation of Assam tea leaves with mixed microbial culture involving lactic acid bacteria and yeast. Miang has important bioactive benefits such as antioxidant and antimicrobial activity with relevance to health benefits. Miang is categorized into two processes; filamentous fungi growth-based (FFP) and non-filamentous...
Mon, 04/22/2019 - 00:00
Toxins, Vol. 11, Pages 233: Structural Insights to the Heterotetrameric Interaction between the Vibrio parahaemolyticus PirAvp and PirBvp Toxins and Activation of the Cry-Like Pore-Forming Domain Toxins doi: 10.3390/toxins11040233 Authors: Shin-Jen Lin Yi-Fan Chen Kai-Cheng Hsu Yun-Ling Chen Tzu-Ping Ko Chu-Fang Lo Han-Ching Wang Hao-Ching Wang Acute hepatopancreatic...
Wed, 04/17/2019 - 20:00
Antimicrobial Resistance, Virulence Determinants, and Biofilm Formation of Enterococcus Species From Ready-to-Eat Seafood Etinosa O. Igbinosa1,2* and Abeni Beshiru1 1Applied Microbial Processes and Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria 2Sustainable Development Office, University of Benin, Benin City,...
Tue, 04/16/2019 - 20:00
Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae Susanne Brenzinger1*, Lizah T. van der Aart1, Gilles P. van Wezel1, Jean-Marie Lacroix2, Timo Glatter3 and Ariane Briegel1* 1Department of Microbial Biotechnology & Health, Institute of Biology Leiden, Leiden University, Leiden, Netherlands 2Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576...
Thu, 04/11/2019 - 20:00
Environmental and Clinical Strains of Vibrio cholerae Non-O1, Non-O139 From Germany Possess Similar Virulence Gene Profiles Keike Schwartz, Jens Andre Hammerl, Cornelia Göllner and Eckhard Strauch* Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany Vibrio cholerae is a natural inhabitant of aquatic ecosystems globally. Strains of the serogroups O1...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Sat, 04/06/2019 - 00:00
Toxins, Vol. 11, Pages 207: Cholera Toxin Encapsulated within Several Vibrio cholerae O1 Serotype Inaba Outer Membrane Vesicles Lacks a Functional B-Subunit Toxins doi: 10.3390/toxins11040207 Authors: Elnaz S. Rasti Angela C. Brown Cholera toxin (CT), the major virulence factor of Vibrio cholerae, is an AB5 toxin secreted through the type II secretion system (T2SS). Upon secretion, the...
Fri, 04/05/2019 - 17:00
Xiaoqin Wang, Zhi-Min Li, Qingyue Li, Mingsong Shi, Lingling Bao, Dingguo Xu, Zhimin Li Fermentation-respiration switch protein (FrsA) was thought to play an important role in controlling the metabolic flux between respiration and fermentation pathways, whereas the biochemical function of FrsA was unclear yet. A gene coding for FrsA protein from Vibrio vulnificus was chemically synthesized. The...
Wed, 04/03/2019 - 01:15
Available online 2 April 2019 Author(s): Xiao Cao, Lichao Zhao, Jingfeng Zhang, Xun Chen, Hui Xie, Yanlei Chang, Li WangAbstractAccurate and highly sensitive detection of viable but nonculturable (VBNC) Vibrio parahaemolyticus is crucial for preventing seafood-related outbreaks. The VBNC state may occur due to bacterial susceptibility to cold shock during food storage. Propidium monoazide (PMA)...
Wed, 04/03/2019 - 00:00
Reliable detection of foodborne pathogen is of great importance in guaranteeing food safety. In the present work, based on the multifunctional properties of platinum-coated gold nanorods (AuNR@Pt), we developed a dual-mode aptasensor based on inner filter effect (IFE) and color change strategies and applied it for Vibrio parahaemolyticus (V. parahaemolyticus) detection. Under...
Tue, 04/02/2019 - 11:02
Bacteria can be social and move in groups. Here, we show that Vibrio parahaemolyticus coordinates motility using the S signal, a cell‐cell signaling molecule that serves as a chemoattractant during swimming and swarming. Two methyl‐accepting chemoreceptors detect this autoinducer. One is necessary for S signal reception by liquid‐grown bacteria, while the other is important for chemotaxis by...
Mon, 04/01/2019 - 11:26
Abstract Viable but nonculturable (VBNC) Vibrio parahaemolyticus cannot be detected by the standard cultivation‐based methods. In this study, commonly used viability assessment methods were evaluated for the detection of Vibrio parahaemolyticus in a VBNC state. V. parahaemolyticus cells exposed to nutrient‐deficiency at cold temperature were used for epifluorescence microscopy with SYTO9 and...
Tue, 03/26/2019 - 12:02
The Bay of Bengal is known as the epicenter for seeding several devastating cholera outbreaks across the globe. Vibrio cholerae, the etiological agent of cholera, has extraordinary competency to acquire exogenous DNA by horizontal gene transfer (HGT) and adapt them into its genome for structuring metabolic processes, developing drug resistance,...
Tue, 03/26/2019 - 10:03
Abstract In this study, we evaluated the temperature requirements for home delivery in Taiwan by considering food safety risks of chilled shrimp that may be contaminated with Vibrio parahaemolyticus. Pathogenic V. parahaemolyticus was chosen because it was the main cause of foodborne outbreaks originating from contaminated seafood in Taiwan. The risk of becoming ill due to consumption of raw...
Sun, 03/24/2019 - 12:06
Ethanolic coconut husk extract (ECHE) could be prepared using ethanol as the extracting solvent. The concentration of ethanol have a great impact on the antibacterial efficacy of ECHE. The ECHE extracted with 60% ethanol demonstrated bactericidal effect on the tested bacteria by causing cell wall damage, which could lead to cell death. Summary Antibacterial properties of ethanolic coconut...
Fri, 03/22/2019 - 01:19
Available online 21 March 2019 Author(s): Nodali Ndraha, Hsin-I. HsiaoAbstractWhile seafood is consumed worldwide, eating raw seafood is more popular in Asian countries. Consuming seafood promotes many health benefits, but is not risk free because it can be contaminated with foodborne pathogens, which can result in disease outbreaks. In this study, we performed the risk assessment of Vibrio...
Wed, 03/20/2019 - 01:13
Md. Ashrafudoulla, Md. Furkanur Rahaman Mizan, Heedae Park, Kye-Hwan Byun, Nari Lee, Si Hong Park, Sang-Do Ha
Tue, 03/19/2019 - 09:47
Abstract Aims This study conducted bacterial community, virulence and antibiogram profiling inside the hindgut and skin of freshly caught hilsa fish and those sold at markets. Methods and Results The results of 16S rRNA‐based high‐throughput sequencing showed a higher number of bacterial genera in marketed fish samples than in fresh fish samples. The total operational taxonomic units (OTU),...
Tue, 03/19/2019 - 01:13
Available online 18 March 2019 Author(s): Julia Mougin, Stéphanie Copin, Daline Bojolly, Virginie Raguenet, Annick Robert-Pillot, Marie-Laure Quilici, Graziella Midelet-Bourdin, Thierry Grard, Maryse Bonnin-JusserandAbstractA single strain of Vibrio parahaemolyticus and a single stain of Vibrio cholerae were isolated from shrimps sourced from seafood processing plants. The isolated strains were...
Mon, 03/18/2019 - 13:04
Abstract We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests...
Mon, 03/18/2019 - 13:04
Abstract We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests...
Mon, 03/18/2019 - 11:00
Summary Type VI secretion systems (T6SSs) are widespread, tightly regulated, protein delivery apparatuses used by Gram‐negative bacteria to outcompete their neighbors. The pathogen, Vibrio parahaemolyticus, encodes two T6SSs. These T6SSs are differentially regulated by external conditions. T6SS1, an antibacterial system predominantly found in pathogenic isolates, requires warm marine‐like...
Sat, 03/16/2019 - 01:14
Available online 14 March 2019 Author(s): Gang Zhang, Kaiwen Sun, Guomin Ai, Jianjuan Li, Na Tang, Yuqin Song, Chao Wang, Jie FengAbstractIntrinsic resistance of bacteria to antibiotics plays an increasingly significant role in antibiotic resistance. However, the breadth of intrinsic resistance has not been fully elucidated. Here we identified a novel class of chloramphenicol acetyltransferase (...
Fri, 03/15/2019 - 17:00
Diah Ayuningrum, Yang Liu, Riyanti, Mada T. Sibero, Rhesi Kristiana, Meezan A. Asagabaldan, Zerlina G. Wuisan, Agus Trianto, Ocky Karna Radjasa, Agus Sabdono, Till F. Schäberle Tunicates (Ascidians, sea squirts) are marine protochordates, which live sedentary or sessile in colonial or solitary forms. These invertebrates have to protect themselves against predators and invaders. A most successful...
Fri, 03/15/2019 - 15:30
Vibrio cholerae is an aquatic bacterium with the potential to infect humans. This study focuses on the DNA replication and cell division of this bi‐chromosomal bacterium during starvation stress. Surprisingly, cells temporarily restart their DNA replication after a first shut‐down period before finally reaching a state with fully replicated single chromosome sets. Abstract Vibrio cholerae is...
Fri, 03/15/2019 - 01:12
Available online 13 March 2019 Author(s): F.F. Parlapani, S. Michailidou, D.A. Anagnostopoulos, S. Koromilas, K. Kios, K. Pasentsis, F. Psomopoulos, A. Argiriou, S.A. Haroutounian, I.S. BoziarisAbstractBacterial communities composition using 16S Next Generation Sequencing (NGS) and Volatile Organic Compounds (VOCs) profile of whole blue crabs (Callinectes sapidus) stored at 4 and 10 °C (proper...
Wed, 03/13/2019 - 11:47
Summary Vibrio parahaemolyticus, the leading cause of seafood‐associated gastroenteritis worldwide, requires the two type‐III secretion systems (T3SS1 and T3SS2) and a thermostable direct hemolysin (encoded by tdh1 and tdh2) for full virulence. The tdh genes and the T3SS2 gene cluster constitute an 80 kb pathogenicity island known as Vp‐PAI located on the chromosome II. Expression of T3SS1 and...
Wed, 03/13/2019 - 11:47
Summary Vibrio parahaemolyticus, the leading cause of seafood‐associated gastroenteritis worldwide, requires the two type‐III secretion systems (T3SS1 and T3SS2) and a thermostable direct hemolysin (encoded by tdh1 and tdh2) for full virulence. The tdh genes and the T3SS2 gene cluster constitute an 80 kb pathogenicity island known as Vp‐PAI located on the chromosome II. Expression of T3SS1 and...
Mon, 03/11/2019 - 11:32
The phage shock protein (Psp) system is a stress response pathway that senses and responds to inner membrane damage. The genetic components of the Psp system are present in several clinically relevant Gram-negative bacteria, including Vibrio cholerae. However, most of the current knowledge about the Psp response stems from in vitro studies in Escherichia coli and Yersinia enterocolitica. In fact...
Mon, 03/11/2019 - 11:32
Korormicin is an antibiotic, produced by some Pseudoalteromonads, which selectively kills gram negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that, although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Instead, perturbation of electron transfer inside the enzyme promotes a reaction...
Fri, 03/08/2019 - 01:13
Publication date: Available online 6 March 2019Source: LWTAuthor(s): Piyanan Chuesiang, Ubonrat Siripatrawan, Romanee Sanguandeekul, Jason Szuhao Yang, David Julian McClements, Lynne McLandsboroughAbstractThe water-dispersibility and antimicrobial activity of cinnamon oil is enhanced by encapsulating it within oil-in-water nanoemulsions. This study investigated the impact of oil phase composition...
Thu, 03/07/2019 - 17:00
Jae Seung Yang, So Jung An, Mi Seon Jang, Manki Song, Seung Hyun Han Serum vibriocidal antibody assays have long been used to evaluate the immunogenicity of cholera vaccines formulated with killed whole-cell Vibrio cholerae. However, the antibody isotypes responsible for the serum vibriocidal activity are not fully characterized. In this study, we examined 20 clinical serum samples obtained from...
Tue, 03/05/2019 - 12:02
The Feo ferrous iron transporter is widely distributed among bacteria and archaea, but its mechanism of transport has not been fully elucidated. In Vibrio cholerae, the transport system requires three proteins: the small cytosolic proteins FeoA and FeoC and a large cytoplasmic-membrane–associated protein FeoB, which has an N-terminal G-protein domain....

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