Vibrio

Sat, 10/12/2019 - 03:32
The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n=4), V. alginolyticus (n=11), V. diabolicus (n=14), and V. harveyi (n=3) were detected using gyrB sequencing. The phenotypic pathogenicity revealed DNase, amylase...
Fri, 10/11/2019 - 03:32
The present study assessed the occurrence, virulence determinants and antimicrobial susceptibility patterns of Vibrio spp. isolated from live Manila clam (Ruditapes philippinarum). A total of 31 Vibrio spp. including 27 V. diabolicus, 2 V. fluvialis, 1 V. alginolyticus, and 1 V. antiquarius were isolated and identified. Phenotypic detection of DNase, lipase, phospholipase, amylase, and caseinase...
Sat, 10/05/2019 - 03:39
Author(s): Gao Xiaolong, Ke Caihuan, Zhang Mo, Li Xian, Wu Fucun, Liu YingAbstractThe effects of a diet containing the probiotic Bacillus amyloliquefaciens on the survival and growth of Haliotis discus hannai were evaluated by measuring growth and hematological parameters and the expression levels of nonspecific immune genes. In addition, the abalone's response to Vibrio parahaemolyticus...
Thu, 10/03/2019 - 03:32
Doctor fish (Garra rufa, Heckel, 1843) are increasingly used for cosmetic treatment raising particular concerns regarding the potential transmission of infections to clients. Investigations of microbial causes undertaken in two outbreaks of mortality among G. rufa used for cosmetic treatment revealed the presence of multiple bacteria, including both fish and human pathogens such as Aeromonas...
Wed, 10/02/2019 - 03:42
by Iftekhar Bin Naser, M. Mozammel Hoque, Shah Nayeem Faruque, M. Kamruzzaman, Shinji Yamasaki, Shah M. Faruque Background Toxigenic Vibrio cholerae resides in aquatic reservoirs of cholera-endemic areas mostly in a dormant form known as conditionally viable environmental cells (CVEC) in which the bacteria remain embedded in an exopolysaccharide matrix, and fail to grow in routine bacteriological...
Mon, 09/30/2019 - 16:54
ABSTRACT We have previously identified Vibrio cholerae mutants in which the stress response to subinhibitory concentrations of aminoglycoside is altered. One gene identified, VC1636, encodes a putative DNA/RNA helicase, recently named RadD in Escherichia coli. Here we combined extensive genetic characterization and high-throughput approaches in order to identify partners and molecular mechanisms...
Mon, 09/30/2019 - 16:54
ABSTRACT BolA family proteins are conserved in Gram-negative bacteria and many eukaryotes. While diverse cellular phenotypes have been linked to this protein family, the molecular pathways through which these proteins mediate their effects are not well described. Here, we investigated the roles of BolA family proteins in Vibrio cholerae, the cholera pathogen. Like Escherichia coli, V. cholerae...
Mon, 09/30/2019 - 16:54
ABSTRACT The pathogenic bacterium Vibrio vulnificus exhibits the ability to form biofilm, for which initiation is dependent upon swimming motility by virtue of a polar flagellum. The filament of its flagellum is composed of multiple flagellin subunits, FlaA, -B, -C, and -D. In V. vulnificus genomes, however, open reading frames (ORFs) annotated by FlaE and -F are also present. Although neither...
Sun, 09/29/2019 - 03:31
Author(s): Chao Peng, Dongchang Xie, Chao Zhao, Haidong Xu, Sigang Fan, Lulu Yan, Pengfei Wang, Lihua QiuAbstractAkirin, which are members of the NF-κB signaling pathway, play critical roles in regulating the expression of antimicrobial peptides. In the present study, the Akirin gene from Penaeus monodon was identified from a transcriptome database and designated as PmAkirin. The complete...
Sun, 09/29/2019 - 03:31
Author(s): Xiaoxu Liu, Weiye Li, Lihua Jiang, Zhenming Lü, Minhai Liu, Li Gong, Bingjian Liu, Liqin Liu, Xiaolong YinAbstractLong non-coding RNA refers to an RNA transcript of a non-coding protein with a sequence length greater than 200 bp. More and more reports indicated that lncRNA was involved in the regulation of gene expression as a signalling molecule, an inducing molecule, a leader...
Sun, 09/29/2019 - 03:31
Author(s): Qian Feng, Yueqian Huang, Defu Yao, Chunhua Zhu, Shengkang Li, Hongyu Ma, Jude Juventus Aweya, Yueling ZhangAbstractProtein kinase CK2 (CK2) is a ubiquitous serine/threonine kinase with multiple cellular functions in vertebrates including apoptosis, differentiation, proliferation, survival, tumorigenesis, signal transduction, immune regulation and inflammation. In the current study,...
Sun, 09/29/2019 - 03:31
Author(s): Ruixia Wang, Xiucai Hu, Aijun Lü, Rongrong Liu, Jingfeng Sun, Yeong Yik Sung, Yajiao SongAbstractSkin plays an important role in the innate immune responses of fish, particularly towards bacterial infection. To understand the molecular mechanism of mucosal immunity of fish during bacterial challenge, a de novo transcriptome assembly of crucian carp Carassius auratus skin upon Aeromonas...
Tue, 09/24/2019 - 03:41
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Tue, 09/24/2019 - 03:41
Cyclic di-GMP (c-di-GMP) is a bacterial second messenger molecule that is important in the biology of Vibrio cholerae, but the molecular mechanisms by which this molecule regulates downstream phenotypes have not been fully characterized. We have previously shown that the Vc2 c-di-GMP-binding riboswitch, encoded upstream of the gene tfoY, functions as an off-switch in response to c-di-GMP. However...
Tue, 09/24/2019 - 03:40
Bacteria synthesize amino acids according to their availability in the environment or, in the case of pathogens, within the host. We explored the regulation of the biosynthesis of branched-chain amino acids (BCAAs) (l-leucine, l-valine, and l-isoleucine) in Vibrio alginolyticus, a marine fish and shellfish pathogen and an emerging opportunistic human pathogen. In this species, the ilvGMEDA operon...
Tue, 09/24/2019 - 03:40
The mortality rate associated with Vibrio vulnificus sepsis remains high. An in vitro time-kill assay revealed synergism between tigecycline and ciprofloxacin. The survival rate was significantly higher in mice treated with tigecycline plus ciprofloxacin than in mice treated with cefotaxime plus minocycline. Thus, combination treatment with tigecycline-ciprofloxacin may be an effective novel...
Tue, 09/24/2019 - 03:38
Author(s): Xiaoye Shen, Yi-Cheng Su, Chengchu Liu, Tom Oscar, Angelo DePaola
Tue, 09/24/2019 - 03:37
Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz BanaeiAbstractThough the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for...
Tue, 09/24/2019 - 03:27
Nitric oxide (NO) and its derivatives are important effectors of host innate immunity, disrupting cellular function of infecting pathogens. Transcriptome analysis of Vibrio vulnificus, an opportunistic human pathogen, identified a set of genes induced upon exposure to NO. Among them, VvhmpA (V. vulnificus hmpA), encoding a multidomain NO dioxygenase, was the most greatly induced upon exposure to...
Tue, 09/24/2019 - 03:27
The emerging threat of drug resistant bacteria has prompted the investigation into bacterial signaling pathways responsible for pathogenesis. One such mechanism by which bacteria regulate their physiology during infection of a host is through a process known as quorum sensing (QS). Bacteria use QS to regulate community-wide gene expression in response to changes in population density. In order to...
Mon, 09/23/2019 - 03:31
Author(s): Dinglong Yang, Yijing Han, Lizhu Chen, Ruiwen Cao, Qing Wang, Zhijun Dong, Hui Liu, Xiaoli Zhang, Qianqian Zhang, Jianmin ZhaoAbstractBactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was...
Mon, 09/23/2019 - 03:31
Author(s): Xianyun Ren, Yunbin Zhang, Ping Liu, Jian LiAbstractThis study aimed to use isobaric tags (IBTs) to investigate the immune response of the hepatopancreas of Marsupenaeus japonicas infected with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Liquid chromatography-tandem mass spectrometry and protein sequencing identified 1005 proteins. Among them, 109 proteins were...
Mon, 09/23/2019 - 03:30
Author(s): Haibo Zhou, Ying Liang, Lu Gao, Jianluan Ren, Feng Xue, Dehua Guo, Yuan Jiang, Zhenquan Yang, Lele Lian, Jianjun DaiAbstractQuinolone-resistant foodborne pathogens have become an important public health concern, however, little is known about the molecular mechanism of ciprofloxacin (CIP) resistance among Vibrio parahaemolyticus isolates. This study aimed to explore new genes...
Mon, 09/23/2019 - 03:29
Author(s): Aditi Gangopadhyay, Hirak Jyoti Chakraborty, Abhijit DattaAbstractCholera is a major global threat, affecting millions each year. The ADP ribosyltransferase activity of the active cholera toxin catalyses the massive loss of water and electrolytes during cholera infections. The active toxin heterodimer comprises the A1 subunit from Vibrio cholerae and ARF (ADP Ribosylation Factor) from...
Sun, 09/22/2019 - 03:31
Antibiotic tolerance, the ability to temporarily sustain viability in the presence of bactericidal antibiotics, constitutes an understudied and yet potentially widespread cause of antibiotic treatment failure. We have previously shown that the Gram-negative pathogen Vibrio cholerae can tolerate exposure to the typically bactericidal β-lactam antibiotics by assuming a spherical morphotype...
Sun, 09/22/2019 - 03:30
Author(s): Rasheduzzaman Rashu, Taufiqur Rahman Bhuiyan, Mohammad Rubel Hoq, Lazina Hossain, Anik Paul, Ashraful Islam Khan, Fahima Chowdhury, Jason B. Harris, Edward T. Ryan, Stephen B. Calderwood, Ana A. Weil, Firdausi QadriAbstractVibrio cholerae O1 can cause life threatening diarrheal disease if left untreated. T cells can play critical roles in inducing B cell mediated immunity. As the...
Sun, 09/22/2019 - 03:30
Author(s): Priyadarshini Mukherjee, Vivek Mondal, Ushasi Bhaumik, Ritam Sinha, Sounak Sarkar, Soma Mitra, Debaki Ranjan Howlader, Suhrid Maiti, Asish Kumar Mukhopadhyay, Shanta Dutta, Hemanta KoleyAbstractMultiple diarrheagenic enteric bacterial infections cause global morbidity and mortality. A combination vaccine is needed to combat different diarrhea-causing organisms. In our present work, we...
Sun, 09/22/2019 - 03:30
Author(s): Jing Li, Zhi-Bin Wu, Zhao Zhang, Ji-Wei Zha, Shen-Ye Qu, Xiao-Zhou Qi, Gao-Xue Wang, Fei LingAbstractNowadays, there is no suitable treatment for vibriosis in groupers. So an eco-efficient and environmentally friendly treatment is necessary for the grouper industry. Probiotic-feeding has been a promising strategy to control the bacterial pathogens in aquaculture. A new Bacillus...
Sun, 09/22/2019 - 03:30
Author(s): Phennapa Promthale, Pattira Pongtippatee, Boonsirm Withyachumnarnkul, Kanokpan WongprasertAbstractFishmeal is the main source of protein in the shrimp feed industry and is normally derived from trash fish. As such, the production of fishmeal has an adverse effect on the marine environment by taking away small and juvenile fish, leading to depletion of marine species. There is a need...
Sun, 09/22/2019 - 03:30
Author(s): Mengting Luo, Linwei Yang, Zi-ang Wang, Hongliang Zuo, Shaoping Weng, Jianguo He, Xiaopeng XuAbstractC-type lectins (CTLs) are a group of lectins with at least one carbohydrate recognition domain (CRD), the binding of which to carbohydrates requires the presence of calcium ions. CTLs generally function as pattern recognition receptors (PRRs), essentially participating in innate...
Sun, 09/22/2019 - 03:30
Author(s): Jiquan Zhang, Yujie Liu, Yongzhao Zhou, Wenzheng Wang, Naike Su, Yuying SunAbstractTrehalose, a nonreducing disaccharide, is present in a wide variety of organisms and plays a key role in many organisms under different stress conditions. In the study, the full-length cDNA sequence encoding trehalose-6-phosphate synthase (EcTPS) was obtained from Exopalaemon carinicauda. The complete...
Sun, 09/22/2019 - 03:30
Author(s): Jiajia Yu, Hongxia Wang, Xin Yue, Baozhong LiuAbstractMeretrix petechialis is an important commercial aquaculture species in China. During the clam culture period, mass mortality events often occurred due to the Vibrio infection. In this paper, M. petechialis were challenged with Vibrio parahaemolyticus immersion to simulate a natural infection, and the infection process were divided...
Sun, 09/22/2019 - 03:30
Author(s): Jianning Zhang, Yifei Zhang, Linbao Zhang, Qianyu Wei, Xiaoli Liu, Dinglong YangAbstractIn the present study, a sialic acid-binding lectin was cloned and characterized from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading frame of RpSabl encoded a polypeptide of 162 amino acids with a calculated molecular mass of 17.7 kDa. Analysis of the conserved domain...
Sun, 09/22/2019 - 03:30
Author(s): Werasan Kewcharoen, Prapansak SrisapoomeAbstractAcute hepatopancreatic necrosis disease (AHPND), a serious disease caused by some Vibrio spp., impacts the Pacific white shrimp industry worldwide, especially in Thailand. To effectively overcome this problem, efficacious probiotic candidates were isolated from shrimp farms near coastal areas. The isolated Bacillus probiotics were...
Sun, 09/22/2019 - 03:30
Author(s): Jianning Zhang, Yifei Zhang, Lizhu Chen, Jianmin Yang, Qianyu Wei, Bowen Yang, Xiaoli Liu, Dinglong YangAbstractIn the study, two c-type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin...
Sun, 09/22/2019 - 03:30
Author(s): Crystal Guluarte, Martha Reyes-Becerril, Daniel Gonzalez-Silvera, Alberto Cuesta, Carlos Angulo, María Ángeles EstebanAbstractThe aim of this study was to analyze the probiotic potential, fatty acid composition and immunostimulant activities of Kluyveromyces lactis M3 isolated from a hypersaline sediment. For this purpose, K. lactis M3 resistance to different pH, salinities and bile,...
Sun, 09/22/2019 - 03:30
Author(s): Ming Li, Chunxia Ma, Peng Zhu, Yanhao Yang, Aiyingi Lei, Xiaohan Chen, Wanwen Liang, Ming Chen, Jianha Xiong, Chaozheng LiAbstractCrustin is an antimicrobial peptide (AMP) that plays a key role in the innate immunity of crustaceans. This study cloned a new crustin from Pacific white shrimp Litopenaeus vannamei, which we designated as LvCrustinB, using rapid amplification of cDNA ends (...
Sun, 09/22/2019 - 03:30
Author(s): Shuang Zhang, Chaozheng Li, Wei Wang, Chenggui Wang, Chengbo Sun, Siuming Chan, Lili ShiAbstractProtein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals and Drosophila, but the function of PIAS in crustaceans is still...
Sun, 09/22/2019 - 03:26
The phenotypic characteristics and growth kinetics at several temperatures, salinities, and pH values of three Vibrio parahaemolyticus (Vp) strains with different virulence and one nonpathogenic strain were evaluated. Independent of the virulence of the strain, a high metabolic diversity was found, which yielded different colored phenotypes on the CHROMagar™ Vibrio. All strains were resistant to...
Sun, 09/22/2019 - 03:26
Acute hepatopancreatic necrosis disease (AHPND) causes massive mortality in shrimp ponds within the first month poststocking. The causative agent is a specific strain of Vibrio parahaemolyticus (VPAHPND) that has acquired the capability to produce virulent binary toxins called ToxA and ToxB. This study aims to test the effect of the addition of an autoinducer‐2‐containing cell‐free supernatant (...
Sun, 09/22/2019 - 03:26
In this study, the antibacterial properties of Pimpinella anisum essential oil (PAO) were evaluated against six food‐related bacteria. Afterward, the effects of different concentrations of PAO on microbial, chemical, and organoleptic properties of minced beef were evaluated during refrigerated storage. Based on the findings, PAO was effective against all of the tested strains, where minimum...
Sun, 09/22/2019 - 03:24
Aims:  To develop simple and rapid PCR‐fingerprinting methods for Vibrio cholerae O1 (El Tor and classical biotypes) and O139 serogroup strains which cause major cholera epidemics, on the basis of the diversity of superintegron (SI) carried by these strains. Methods and Results:  PCR‐restriction fragment length polymorphism (PCR‐RFLP) assay was developed targeting region between integrase gene in...
Sun, 09/22/2019 - 03:24
In the chicken slices and canned beef B.bacteriovorax induced a E.coli reduction equal to 4.3 and 2.1 logs, respectively, at 6 hours from contamination.♦ E. coli control; ● E. coli test; ▲ Bb control; ■ Bb test. Summary Bdellovibrio bacteriovorus is a predator micro‐organism towards other Gram‐negative bacteria. We tested B. bacteriovorus to control Escherichia coli growth on chicken slices and...
Sun, 09/22/2019 - 03:24
Kenya is endemic for cholera with different waves of outbreaks having been documented since 1971. In recent years, new variants of Vibrio cholerae O1 have emerged and have replaced most of the traditional El Tor biotype globally. These strains also appear to have increased virulence, and it is important to describe and document their phenotypic and genotypic traits. This study characterized 146 V...
Sun, 09/22/2019 - 03:22
This paper aims to characterize halophilic bacteria inhabiting Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria). In addition, screening of enzymatic activities, heavy metal tolerance and antagonistic potential against phytopathogenic fungi were tested. A total of 74 bacterial isolates were screened and phylogenetically...
Sun, 09/22/2019 - 03:22
Background The ability to identify bacterial pathogens that necessitate specific clinical management or public health action in children with acute gastroenteritis is crucial to patient care and public health. However, existing stool-testing guidelines offer inconsistent recommendations, and their performance characteristics are unknown. We evaluated 6 leading gastroenteritis guidelines (eg,...
Thu, 09/19/2019 - 14:29
Available online 13 September 2019 Author(s): Nan Ling, Jinling Shen, Jingjing Guo, Dexin Zeng, Jianluan Ren, Lixin Sun, Yuan Jiang, Feng Xue, Jianjun Dai, Baoguang LiAbstractVibrio parahaemolyticus is an important human pathogen causing a variety of life-threatening diseases and is widely distributed in marine and estuarine environments. The objective of this study was to develop a sensitive,...

Pages