Vibrio

Sat, 02/15/2020 - 03:37
by Samit Watve, Kelsey Barrasso, Sarah A. Jung, Kristen J. Davis, Lisa A. Hawver, Atul Khataokar, Ryan G. Palaganas, Matthew B. Neiditch, Lark J. Perez, Wai-Leung Ng Many bacteria use quorum sensing (QS) to regulate virulence factor production in response to changes in population density. QS is mediated through the production, secretion, and detection of signaling molecules called autoinducers (...
Sat, 02/15/2020 - 03:36
Author(s): Ryunkyoung Oh, Min Jeong Lee, Young-Ok Kim, Bo-Hye Nam, Hee Jeong Kong, Ju-Won Kim, Jung-yeon Park, Jung-Kil Seo, Dong-Gyun KimAbstractWe isolated and purified an antimicrobial peptide (AMP) from the mantle of the hard-shelled mussel, Mytilus coruscus. The peptide was purified through C18 reversed-phase high-performance liquid chromatography, and displayed antibacterial activity. Total...
Sat, 02/15/2020 - 03:27
The caspase family proteins are aspartate‐specific cysteine proteases that transmit extracellular signals to cells, ultimately cause apoptosis and therefore play a key role in cellular immunity. In this study, we cloned and characterized three caspases from Chinese black sleeper (Bostrychus sinensis), Bscasp‐1, Bscasp‐8 and Bscasp‐9. Real‐time PCR analysis showed that Bscasp‐1, Bscasp‐8 and...
Thu, 02/13/2020 - 03:47
Chitosan with higher molecular weight exhibited higher antimicrobial efficacy against foodborne pathogens. However, the poor water solubility of higher or medium molecular weight chitosan limits its applications. To overcome the challenge, our research team was searching for simple preparation procedure for fast dissolving MMW chitosan in water. Through the process, we were also able to obtain a...
Wed, 02/12/2020 - 10:50
Author(s): Jiahua Xing, Jia Yu, Yin LiuAbstractVibrio parahaemolyticus, a major food-borne pathogen, is a gram-negative rod-shaped halophilic bacterium which inhabits marine environments throughout the world. It can pose a threat to humans after the consumption of raw or undercooked seafood. Fast detection is crucial for hindering and controlling V. parahaemolyticus infection. Compared with...
Wed, 02/12/2020 - 03:25
The subject of analysis in this report was the antibiotic susceptibility of V. cholerae under glucose supplementation since the metabolites can significantly alter the antibiotic sensitivity of bacteria. Glucose could change the antibiotic susceptibility in a growth phase-dependent manner, however, the antibiotic susceptibility of exponentially growing cells was not affected in the presence of...
Mon, 02/10/2020 - 04:11
Author(s): Xu Li, Lin Zhao, He Gao, Long Chen, Fenxia Fan, Zhe Li, Yufeng Fan, Jie Li, Weili Liang, Bo Pang, Biao KanAbstractIn Vibrio cholerae, the lysogenic bacteriophage CTXΦ carries the cholera toxin genes ctxAB, which can be transferred from toxigenic strains to nontoxigenic strains through infection and lysogenic conversion of CTXΦ. This phage also has the precursor genome which does not...
Mon, 02/10/2020 - 04:09
Author(s): Rafaela C. Baptista, Heber Rodrigues, Anderson S. Sant'AnaAbstractThe present work aimed to evaluate the frequency of seafood consumption by the Brazilian population, the safety practices during seafood handling and preservation as well as to evaluate the consumers’ perception on the risks associated with these foods. A total of 962 respondents responded to a survey divided into three...
Fri, 02/07/2020 - 04:16
Author(s): Zhuoxing Zhang, Chao Zhang, Xiaoling Dai, Ruidong Zhang, Xueying Cao, Kaiqiang Wang, Xin Huang, Qian RenAbstractNuclear factor κB (NF-κB) plays a key role in the innate immunity of invertebrates. Relish belongs to the NF-κB family. In insects, alternative splicing induces the sequence diversity of the Relish gene. However, information on the roles of various relish isoforms in...
Fri, 02/07/2020 - 04:16
Author(s): Le Fei Jiao, Tian Meng Dai, Sun Qian Zhong, Min Jin, Peng Sun, Qi Cun ZhouAbstractThe intestine is the primary target of pathogenic microbes during invasion. However, the interaction of Vibrio parahaemolyticus (V. parahaemolyticus) with intestinal epithelial cells and its effects on the intestinal function of Litopenaeus vannamei (L. vannamei) are poorly studied. Therefore, the aim of...
Wed, 02/05/2020 - 03:27
The objective of this study was to determine whether seeds of Brassica oleracea var. italica (i.e. broccoli, an edible plant) produce defensins that inhibit phytopathogenic fungi and pathogenic bacteria of clinical significance. Crude extracts obtained from broccoli seeds were fractioned by molecular exclusion techniques and analyzed by liquid chromatography-high-resolution mass spectrometry. Two...
Wed, 02/05/2020 - 03:26
Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific...
Mon, 02/03/2020 - 03:34
Author(s): Yan-li Su, Guang-hua Wang, Jing-jing Wang, Bing Xie, Qin-qin Gu, Dong-fang Hao, Hong-mei Liu, Min ZhangAbstractAt present, several reports have indicated that the C-terminal peptides of tissue factor pathway inhibitor 1 (TFPI-1) were active antibacterial peptides. However, the functions of TFPI-1 C-terminal peptides in teleost are still very limited. In this study, a C-terminal peptide...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:25
Vibrio parahaemolyticus (Vp), a major food-borne pathogen, is responsible for severe infections such as gastroenteritis and septicemia, which may be accompanied by life-threatening complications. While studies have evaluated factors that affect the virulence of the pathogen, none have investigated the interaction of Vp with gut microbiota. To address this knowledge gap, we compared the effect of...
Thu, 01/30/2020 - 03:25
An estimated 700 000 soldiers belonging to the Union Army and the Confederate States Army died in the 1861–65 American Civil War.1 Many camp epidemics attributed to dysentery, typhoid fever, measles, smallpox, cholera, and malaria spread quickly among the troops, affecting the Confederate Army more and surely to some extent shaping the outcome of the war.2
Wed, 01/29/2020 - 04:01
Author(s): Nguyen Nhat Minh Phuong, Thien Trung Le, John Van Camp, Katleen RaesAbstractMicrobial safety in food products is not always adequately controlled. Chemical antimicrobials which are recognized as hazards to human health are gradually replaced by natural antimicrobial compounds. In the current study, the antimicrobial activity against some Gram-positive and Gram- negative bacteria by the...
Sun, 01/26/2020 - 03:27
Aim The current study was conducted to determine the incidence, antibiotic resistance and virulence genes of Vibrio strains isolated from ready‐to‐eat shrimps in Edo and Delta States, Nigeria. Methods and Results A total of 1440 ready‐to‐eat shrimp's samples were obtained from open markets from November 2016 to October 2017 and analysed using standard culture‐based procedures. Overall, our result...
Fri, 01/24/2020 - 03:33
Author(s): Junjie Nie, Zhixue Yu, Defu Yao, Fan Wang, Chunhua Zhu, Kaihui Sun, Jude Juventus Aweya, Yueling ZhangAbstractThe histone deacetylase, sirtuin 6 (SIRT6), plays an essential role in the regulation of oxidative stress, mitochondrial function and inflammation in mammals. However, the specific role of SIRT6 in invertebrate immunity has not been reported. Here, we characterized for the...
Fri, 01/24/2020 - 03:26
Populations of the bacterium Vibrio cholerae consist of dozens of distinct lineages, with primarily (but not exclusively) members of the pandemic generating (PG) lineage capable of causing the diarrheal disease cholera. Assessing composition and temporal dynamics of such populations requires extensive isolation efforts and thus only rarely covers large geographic areas or timeframes exhaustively...
Sat, 01/18/2020 - 03:23
Biofilms provide bacteria with protection from environmental stresses and host immune defenses. The pathogenic marine bacterium Vibrio vulnificus forms biofilms and colonizes environmental niches such as oysters. The cabABC operon encodes an extracellular matrix protein CabA and the corresponding type I secretion system, which are essential for biofilm and rugose colony development of V....
Wed, 01/15/2020 - 03:25
Nanogold enhanced surface plasmon resonance (SPR), colloidal gold immunochromatographic test strips (ICTS), and polymerase chain reaction (PCR), combined with immunomagnetic separation (IMS) were established in this study for the rapid detection of Vibrio parahaemolyticus (VP). The sensitivities of SPR, ICTS, and PCR was determined to be 101, 103, and 103 CFU/mL for VP, respectively. After...
Tue, 01/14/2020 - 03:34
The marine bacterium and human pathogen Vibrio parahaemolyticus rapidly colonizes surfaces by using swarming motility and forming robust biofilms. Entering either colonization program, swarming motility or sessility, involves differential regulation of many genes resulting in a dramatic shift in physiology and behavior. V. parahaemolyticus has evolved complex regulation to control these two...
Thu, 01/09/2020 - 04:05
Author(s): Hourong Liu, Chengwen Song, Junhao Ning, Yuan Liu, Zhaoxia CuiAbstractVariable lymphocyte receptors (VLRs) play an important role via their antigen-special reorganization in jawless vertebrates (agnathans) adaptive immune response. In the present study, the open reading frame (ORF) of Eriocheir sinensis VLRA (designated as EsVLRA) was identified. EsVLRA comprised a 799-amino-acid...
Wed, 01/08/2020 - 03:40
Steryl esters (SEs) are important storage compounds in many eukaryotes and are often prominent components of intracellular lipid droplets. Here, we demonstrate that selected Actino- and Proteobacteria growing on sterols are also able to synthesize SEs and to sequester them in cytoplasmic lipid droplets. We found cholesteryl ester (CE) formation in members of the actinobacterial genera Rhodococcus...
Wed, 01/08/2020 - 03:39
Author(s): Xifeng Qiao, Pingchao Li, Jianan He, Zeshu Yu, Jiaxing Chen, Liangge He, Xue Yu, Haoran Lin, Danqi Lu, Yong ZhangAbstractScavenger receptors play a central role in defending against infectious diseases in mammals. However, the function of SRECII remains unknown in teleost fish. In this study, type F scavenger receptor expressed by endothelial cells-II (SRECII) cDNA sequence was first...
Wed, 01/08/2020 - 03:27
M. Abanto et al. We report transcontinental expansion of Vibrio parahaemolyticus sequence type 36 into Lima, Peru. From national collections, we identified 7 isolates from 2 different Pacific Northwest complex lineages that surfaced during 2011–2016. Sequence type 36 is likely established in environmental reservoirs. Systematic surveillance enabled detection of these epidemic isolates.
Sat, 01/04/2020 - 03:36
Author(s): Chao Zhang, Xueying Cao, Kaiqiang Wang, Xiaoling Dai, Ruidong Zhang, Zhuoxing Zhang, Xin Huang, Qian RenAbstractTranscription factor activator protein 1 (AP1) plays an irreplaceable role in the response to a variety of external stimulants, such as cellar stress, bacterial and viral infections, and inflammatory cytokines. In this study, we identified a novel AP1 gene from Macrobrachium...
Sat, 01/04/2020 - 03:36
Author(s): Xiaoling Dai, Xin Huang, Zhuoxing Zhang, Ruidong Zhang, Xueying Cao, Chao Zhang, Kaiqiang Wang, Qian RenAbstractInnate immunity is the primary defense of crustaceans against pathogens. Crustins, as antimicrobial peptides, are important to crustacean innate immunity. In this study, two kinds of Gly-rich crustin genes were cloned from Macrobrachium nipponense and were referred to as Mn-...
Sat, 01/04/2020 - 03:36
Author(s): Lei Liu, Yunfei DangAbstractMannose-binding lectin (MBL) is a crucial pattern recognition receptor in the host innate immune system. Previously, we reported the biological function of Ctenopharyngodon idella MBL (CiMBL) in initiating the lectin pathway of the complement system. In the present study, we further explored its biological function including the agglutinating ability,...
Wed, 12/25/2019 - 03:34
ABSTRACT The dinucleotide second messenger c-di-GMP has emerged as a central regulator of reversible cell attachment during bacterial biofilm formation. A prominent cell adhesion mechanism first identified in pseudomonads combines two c-di-GMP-mediated processes: transcription of a large adhesin and its cell surface display via posttranslational proteolytic control. Here, we characterize an...
Wed, 12/25/2019 - 03:34
ABSTRACT The Gram-negative marine bacterium Vibrio parahaemolyticus is a common cause of infectious gastroenteritis due to the ingestion of contaminated seafood. Most virulent V. parahaemolyticus strains encode two type III secretion systems (T3SS1 and T3SS2); however, the roles they and their translocated effectors play in causing intestinal disease remain unclear. While studies have identified...
Wed, 12/25/2019 - 03:34
ABSTRACT In Vibrio species, chitin-induced natural transformation enables bacteria to take up DNA from the external environment and integrate it into their genome. Expression of the master competence regulator TfoX bypasses the need for chitin induction and drives expression of the genes required for competence in several Vibrio species. Here, we show that TfoX expression in Vibrio campbellii...
Tue, 12/24/2019 - 03:34
The intrinsic resistance of Pseudomonas aeruginosa to many antibiotics limits treatment options for pseudomonal infections. P. aeruginosa's outer membrane is highly impermeable and decreases antibiotic entry into the cell. We used an unbiased, high-throughput approach to examine mechanisms underlying outer membrane-mediated antibiotic exclusion. Insertion Sequencing (INSeq) identified genes that...
Tue, 12/24/2019 - 03:26
Vibrio cholerae is a waterborne bacterium responsible for worldwide outbreaks of acute and fatal cholera. Recently, small regulatory RNAs (sRNAs) have become increasingly recognized as important regulators of virulence gene expression in response to environmental signals. In this study, we determined that two component system EnvZ/OmpR was required for intestinal colonization in V. cholerae O1 EI...
Tue, 12/24/2019 - 03:26
Bacteria in nature are widely exposed to differential fluid shears which are often a trigger for phenotypic switches. The latter mediates transcriptional and translation remodeling of cellular metabolism impacting among others virulence, antimicrobial resistance and stress resistance. In this study, we evaluated the role of fluid shear on phenotypic switch in an acute hepatopancreatic necrosis...
Sun, 12/22/2019 - 03:23
Vibrioparahaemolyticus is a leading seafood-borne pathogen that causes gastroenteritis, septicemia, and serious wound infections due to the actions of virulence-associated proteins. We compared the extracellular proteins of nonvirulent JHY20 and virulent ATCC 33847 V. parahaemolyticus reference strains. Eighteen extracellular proteins were identified from secretory profiles, and 11 (68.75%) of...
Sat, 12/21/2019 - 04:05
Author(s): Le He, LiQun Wu, YiJun Tang, Peng Lin, ShaoWei Zhai, YiQun Xiao, SongLin GuoAbstractIn cultivated European eels, Aeromonas hydrophila, Edwardsiella anguillarum and Vibrio vulnificus are three important bacterial pathogens. In this study, an expressed recombinant Outer membrane proteinⅡ (rOmpⅡ) from A. hydrophila was intraperitoneally injected into European eels (Angullia angullia). All...
Fri, 12/20/2019 - 03:28
Vibrio parahaemolyticus is a leading cause of seafood‐borne bacterial gastroenteritis in humans. Since its discovery in 1950, this bacterium has been isolated in widespread outbreaks and in sporadic cases of gastroenteritis worldwide. Although the exotoxin, thermostable direct hemolysin (TDH), had been the focus of extensive research on the pathogenicity of V. parahaemolyticus, the whole‐genome...
Fri, 12/20/2019 - 03:26
Vibrio cholerae is a leading waterborne pathogenic bacterium worldwide. It can cause human cholera that is still pandemic in developing nations. Detection of V. cholerae contamination in drinking water and aquatic products is imperative for assuring food safety. In this study, a simple, sensitive, specific, and visualized method was developed based on loop-mediated isothermal amplification (LAMP...
Fri, 12/20/2019 - 03:26
ICEpMERPH, the first integrative conjugative element (ICE) of the SXT/R391 family isolated in the United Kingdom and Europe, was analyzed to determine the nature of its adaptive functions, its genetic structure, and its homology to related elements normally found in pathogenic Vibrio or Proteus species. Whole genome sequencing of Escherichia coli (E. coli) isolate K802 (which contains the...
Thu, 12/19/2019 - 03:29
Reduced digestibility of foods containing oxidized proteins and the subsequent excessive accumulation of undigested components in the colon may cause changes in the intestinal flora composition. This study evaluates the characteristics of this change and the potential adverse effects on organisms. Methods and results Pork was cooked using sous‐vide or at high temperature and pressure (HTP), and...
Wed, 12/18/2019 - 04:04
Author(s): Xiao-hong Sun, Li-ran Hao, Qing-chao Xie, Wei-qing Lan, Yong Zhao, Ying-jie Pan, Vivian C.H. WuAbstractThis study aimed to investigate the antimicrobial effect and membrane damage mechanism of blueberry extract against Vibrio parahaemolyticus. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of blueberry extract against three strains of V....
Mon, 12/16/2019 - 03:33
Author(s): Songlin Guo, Le He, Liqun Wu, Yiqun Xiao, Shaowei Zhai, Qinpi YanAbstractIn cultivated European eels, Aeromonas hydrophila, Edwardsiella anguillarum and Vibrio vulnificus are three important bacterial pathogens. In this study, European eels (Anguilla anguilla) were immunized by the bivalent expression products of the outer membrane protein (Omp) gene from A. hydrophila (OmpⅡ) and E....
Sun, 12/15/2019 - 03:36
Author(s): Yuying Sun, Congcong Yan, Mengfei Liu, Yujie Liu, Wenzheng Wang, Wei Cheng, Fusheng Yang, Jiquan ZhangAbstractDuring the immune defense reaction of invertebrate, a plenty of reactive oxygen species (ROS) could be induced to product. Though ROS can kill foreign invaders, the accumulation of these reactive molecules in animals will cause serious cell damage. Carotenoids could function as...
Sun, 12/15/2019 - 03:33
Author(s): Xuetong Li, Yuan Su, Huashuo Chu, Shuxia Lyu, Jingjing Tian, Wentao XuAbstractVibrio parahaemolyticus is a major hidden danger of food safety. To develop a rapid, sensitive and on-site detecting method of Vibrio parahaemolyticus (V. parahaemolyticus), a strand replacement primer thermostat phosphate (SRPP) visual sensor was proposed, based on Bst DNA polymerase and pyrophosphatase. The...
Fri, 12/13/2019 - 03:34
Cholera caused by the Gram-negative bacterium Vibrio cholerae still remains a major health burden in developing countries due to its high transmissibility and multidrug resistance. Alternative strategies are in quest to curtail the disease focusing on antivirulent approaches, such as biofilm inhibition, which make bacteria more susceptible to antibiotic therapies. The biofilm state is important...
Wed, 12/11/2019 - 03:50
Y. Tu et al. We investigated the epidemiology of cholera in Taiwan during 2002–2018. Vibrio cholerae sequence type (ST) 75 clone emerged in 2009 and has since become more prevalent than the ST69 clone from a previous pandemic. Closely related ST75 strains have emerged in 4 countries and may now be widespread in Asia.

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