Vibrio

Sat, 03/28/2020 - 03:27
Bacteria constantly experience changes to their external milieu and need to adapt accordingly to ensure their survival. Certain bacteria adapt by means of cellular differentiation, resulting in the development of a specific cell type that is specialized for life in a distinct environment. Furthermore, to understand how bacteria adapt, it is essential to appreciate the significant changes that...
Sat, 03/28/2020 - 03:24
Vibrio vulnificus is a zoonotic pathogen that lives in temperate, tropical and subtropical aquatic ecosystems whose geographical distribution is expanding due to global warming. The species is genetically variable and only the strains that belong to the zoonotic clonal-complex can cause vibriosis in both humans and fish (being its main host the eel). Interestingly, the severity of the vibriosis...
Fri, 03/27/2020 - 03:51
The combination of nisin, EDTA, and sulfite at a low dose of 0.625% exhibited higher antimicrobial activity than did a high dose of sulfite (1.25%) for shrimp preservation. Based on aerobic bacteria counts, psychrotrophic bacteria, and TVB‐N, shrimp treatment with combination of nisin, EDTA, and low‐dose sulfite were still acceptable within 10 days of storage. Abstract This study aims to...
Wed, 03/25/2020 - 03:34
Vibrio cholerae (V. cholerae) uses the autoinducer CAI-1 (cholera autoinducer 1) and several linked quorum sensing systems in order to efficiently sense its ever-changing environment and optimally coordinate population-wide gene expression. Indole has been reported as an important signal that is sensed by V. cholerae, and here, we report the synthesis and evaluation of a focused library of...
Tue, 03/24/2020 - 03:42
V. parahaemolyticus is autochthonous to the marine environment and causes seafood-borne gastroenteritis in humans. Generally, V. parahaemolyticus recovered from the environment and/or seafood is thought to be non...
Thu, 03/19/2020 - 03:34
ABSTRACT Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causes acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulence factors, including type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis...
Wed, 03/18/2020 - 03:34
ABSTRACT Vibrio parahaemolyticus is an important foodborne pathogen and has recently gained particular notoriety because it causes acute hepatopancreatic necrosis disease (AHPND) in shrimp, which has caused significant economic loss in the shrimp industry. Here, we report a whole-genome analysis of 233 V. parahaemolyticus strains isolated from humans, diseased shrimp, and environmental samples...
Tue, 03/17/2020 - 03:34
by Michael G. Jobling, Steven T. Poole, Fatima Rasulova-Lewis, Aisling O’Dowd, Annette L. McVeigh, Amit Balakrishnan, Stephanie A. Sincock, Michael G. Prouty, Randall K. Holmes, Stephen J. Savarino Surface-expressed colonization factors and their subunits are promising candidates for inclusion into a multivalent vaccine targeting enterotoxigenic Escherichia coli (ETEC), a leading cause of acute...
Sat, 03/14/2020 - 03:25
The “Photorhabdus virulence cassettes” (PVCs) secreted by Photorhabdus are defined as “extracellular contractile injection systems” (eCISs) and can deliver effectors to eukaryotic hosts for cytotoxicity. Previously, we demonstrated the cryogenic electron microscopy (cryo-EM) structure and assembly process of an intact PVC particle from Photorhabdus asymbiotica. In this work, we characterized the...
Wed, 03/11/2020 - 03:26
Sea turtles can be considered a sentinel species for monitoring the health of marine ecosystems, acting, at the same time, as a carrier of microorganisms. Indeed, sea turtles can acquire the microbiota from their reproductive sites and feeding, contributing to the diffusion of antibiotic-resistant strains to uncontaminated environments. This study aims to unveil the presence of antibiotic-...
Tue, 03/10/2020 - 03:26
The epidemic diarrheal disease cholera is caused by the Gram-negative bacterium Vibrio cholerae. V. cholerae virulence factors include the toxin-coregulated pilus (TCP) and cholera toxin, which are major factors responsible for host colonization and production of diarrhea. Expression of cholera toxin and TCP genes is controlled by the ToxR regulon. The ToxR regulon includes the transcriptional...
Fri, 03/06/2020 - 04:07
Vibrio cholerae is an aquatic organism and facultative human pathogen that typically resides in coastal areas and brackish water. Here, we report the complete genome sequence of V. cholerae strain RFB16, which was isolated from a freshwater lake in southwestern Pennsylvania.
Thu, 03/05/2020 - 03:30
The Type VI Secretion System (T6SS) is a proteinaceous weapon used by many Gram‐negative bacteria to deliver toxins into adjacent target cells. Vibrio cholerae, the bacterium responsible for the fatal water‐borne cholera disease, uses the T6SS to evade phagocytic eukaryotes, cause intestinal inflammation, and compete against other bacteria with toxins that disrupt lipid membranes, cell walls and...
Wed, 03/04/2020 - 04:07
Helical cell shape appears throughout the bacterial phylogenetic tree. Recent exciting work characterizing cell shape mutants in a number of curved and helical Proteobacteria is beginning to suggest possible mechanisms and provide tools to assess functional significance. We focus here on Caulobacter crescentus, Vibrio cholerae, Helicobacter pylori, and Campylobacter jejuni, organisms from three...
Wed, 03/04/2020 - 01:19
The microbiological quality of ready-to-eat (RTE) foods from school cafeterias in Chongqing, China, was evaluated in comparison to a guideline published by a provincial health commission. Based on their preparation processes and potential risks, the RTE foods were divided into 5 types: food type 1, general cooked and hot-held foods; food type 2, cooked meats; food type 3, heated aquatic products...
Tue, 03/03/2020 - 03:27
Sea level rise and the anthropogenic warming of the world's oceans is not only an environmental tragedy, but these changes also result in a significant threat to public health. Along with coastal flooding and the encroachment of saltwater farther inland comes an increased risk of human interaction with pathogenic Vibrio species, such as Vibrio cholerae, V. vulnificus and V. parahaemolyticus. This...
Tue, 03/03/2020 - 03:25
Vibrio parahaemolyticus is a food-borne pathogen that causes pathogenic symptoms such as diarrhea and abdominal pain. Currently no studies have shown that either pathogenic and non-pathogenic V. parahaemolyticus possess growth heterogeneity in a human environment, such as in gastric and intestinal fluids. The tlh gene is present in both pathogenic and non-pathogenic V. parahaemolyticus strains,...
Sun, 03/01/2020 - 03:35
Author(s): Shuwen Lei, Xiaokui Gu, Qingping Zhong, Linjie Duan, Aimei Zhou Vibrio parahaemolyticus, a marine food-borne pathogen, has been proved to be a significant cause of human gastrointestinal disorders worldwide. In this study, a method of multiplex droplet digital PCR (ddPCR) based on primer and probe sequences of the tlh, tdh and ureR genes were developed and evaluated for the reliable...
Wed, 02/26/2020 - 03:36
ABSTRACT The temporal switching of serotypes from serotype Ogawa to Inaba and back to Ogawa was identified in Vibrio cholerae O1, which was responsible for seasonal outbreaks of cholera in Dhaka during the period 2015 to 2018. In order to delineate the factors responsible for this serotype transition, we performed whole-genome sequencing (WGS) of V. cholerae O1 multidrug-resistant strains...
Wed, 02/26/2020 - 03:34
Author(s): Yibei Zhang, Huanhuan Liu, Dan Gu, Xingxu Lu, Xiaohui Zhou, Xiaodong XiaVibrio parahaemolyticus is a common foodborne pathogen in seafood and represents a major threat to human health worldwide. In this study, we identified that PhoR, a histidine kinase, is involved in the regulation of swarming and flagella assembly. RNA sequencing analysis showed that 1122 genes were differentially...
Wed, 02/26/2020 - 03:29
In Vibrio parahaemolyticus, the iron uptake regulator Fur represses genes involved in iron acquisition and swarming. The Fur regulon also includes a previously uncharacterized regulator fcrX. Overproduction of FcrX, which is a small tetratricopeptide repeat‐containing protein, affects phenotypes in multiple bacterial species. FcrX interacts with V. parahaemolyticus and Escherichia coli Fur....
Mon, 02/24/2020 - 04:11
Author(s): Bowen Chen, Jiaming Huang, Huihui Li, Qiao-Hui Zeng, Jing Jing Wang, Haiquan Liu, Yingjie Pan, Yong ZhaoAbstractVibrio parahaemolyticus is the leading pathogen in seafoods. This study established the efficient blue light-emitting diode (LED) photodynamic inactivation (PDI) to eradicate Vibrio parahaemolyticus and its biofilm with photosensitizer curcumin, and the antibacterial and...
Sun, 02/23/2020 - 03:31
Aims This experimental study focuses on survival and consistence of Vibrio parahaemolyticus in cold water sediments and how increasing temperature and nutritional availability can affect growth. Methods and Results A pathogenic strain of V. parahaemolyticus was inoculated in seawater microcosms containing bottom sediment. Gradually, during 14 days, the temperature was upregulated from 8 to 21°C....
Sat, 02/22/2020 - 03:28
Accumulating evidence has indicated that the multiple drug resistant Vibrio parahaemolyticus may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 and vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged to the Siphoviridae...
Fri, 02/21/2020 - 04:19
Like many other pathogens, Vibrio cholerae, the causative agent of cholera, can modulate its gene expression to combat stresses encountered in both aquatic and host environments, including stress posed by reactive oxygen species (ROS). We previously reported that the virulence activator AphB in V. cholerae is involved in ROS resistance. In this study, we found that another key virulence regulator...
Fri, 02/21/2020 - 04:19
Multidrug efflux systems belonging to the resistance-nodulation-division (RND) superfamily are ubiquitous in Gram-negative bacteria. RND efflux systems are often associated with multiple antimicrobial resistance and also contribute to the expression of diverse bacterial phenotypes including virulence, as documented in the intestinal pathogen Vibrio cholerae, the causative agent of the severe...
Thu, 02/20/2020 - 03:39
Author(s): Xiao-bo Yan, Xiao-hui Dong, Bei-ping Tan, Shuang Zhang, Shu-yan Chi, Qi-huiYang, Hong-yu Liu, Yuan-zhi YangAbstractThe aim of this study was to investigate the effects of feeding alternative dietary oils to hybrid grouper fish (♀Epinephelus fuscoguttatus × ♂E. lanceolatu) on their growth, histological morphology of hepatocytes, disease resistance, immune response, and expression of...
Thu, 02/20/2020 - 03:29
The sessile biofilms of Vibrio parahaemolyticus and Listeria monocytogenes have increasingly become a critical threat in seafood safety. This study aimed to evaluate the effects of modified atmospheres on the formation ability of V. parahaemolyticus and L. monocytogenes biofilms. The stress responses of bacterial biofilm formation to modified atmospheres including anaerobiosis (20% carbon dioxide...
Wed, 02/19/2020 - 03:43
Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesized or isolated from animals, which has restricted its use to small-scale...
Wed, 02/19/2020 - 03:43
This study aimed at establishing the effects of attenuated starters and surface bacteria on various features of caciotta cheese. The cheese undergoes a ripening period during which the house microbiota contaminates the surface. Conventional cheese (the control cheese [CC]) is made using only primary starters. Primary starters and attenuated (i.e., unable to grow and synthesize lactic acid)...
Sat, 02/15/2020 - 03:37
by Samit Watve, Kelsey Barrasso, Sarah A. Jung, Kristen J. Davis, Lisa A. Hawver, Atul Khataokar, Ryan G. Palaganas, Matthew B. Neiditch, Lark J. Perez, Wai-Leung Ng Many bacteria use quorum sensing (QS) to regulate virulence factor production in response to changes in population density. QS is mediated through the production, secretion, and detection of signaling molecules called autoinducers (...
Sat, 02/15/2020 - 03:36
Author(s): Ryunkyoung Oh, Min Jeong Lee, Young-Ok Kim, Bo-Hye Nam, Hee Jeong Kong, Ju-Won Kim, Jung-yeon Park, Jung-Kil Seo, Dong-Gyun KimAbstractWe isolated and purified an antimicrobial peptide (AMP) from the mantle of the hard-shelled mussel, Mytilus coruscus. The peptide was purified through C18 reversed-phase high-performance liquid chromatography, and displayed antibacterial activity. Total...
Sat, 02/15/2020 - 03:27
The caspase family proteins are aspartate‐specific cysteine proteases that transmit extracellular signals to cells, ultimately cause apoptosis and therefore play a key role in cellular immunity. In this study, we cloned and characterized three caspases from Chinese black sleeper (Bostrychus sinensis), Bscasp‐1, Bscasp‐8 and Bscasp‐9. Real‐time PCR analysis showed that Bscasp‐1, Bscasp‐8 and...
Thu, 02/13/2020 - 03:47
Chitosan with higher molecular weight exhibited higher antimicrobial efficacy against foodborne pathogens. However, the poor water solubility of higher or medium molecular weight chitosan limits its applications. To overcome the challenge, our research team was searching for simple preparation procedure for fast dissolving MMW chitosan in water. Through the process, we were also able to obtain a...
Wed, 02/12/2020 - 10:50
Author(s): Jiahua Xing, Jia Yu, Yin LiuAbstractVibrio parahaemolyticus, a major food-borne pathogen, is a gram-negative rod-shaped halophilic bacterium which inhabits marine environments throughout the world. It can pose a threat to humans after the consumption of raw or undercooked seafood. Fast detection is crucial for hindering and controlling V. parahaemolyticus infection. Compared with...
Wed, 02/12/2020 - 03:25
The subject of analysis in this report was the antibiotic susceptibility of V. cholerae under glucose supplementation since the metabolites can significantly alter the antibiotic sensitivity of bacteria. Glucose could change the antibiotic susceptibility in a growth phase-dependent manner, however, the antibiotic susceptibility of exponentially growing cells was not affected in the presence of...
Mon, 02/10/2020 - 04:11
Author(s): Xu Li, Lin Zhao, He Gao, Long Chen, Fenxia Fan, Zhe Li, Yufeng Fan, Jie Li, Weili Liang, Bo Pang, Biao KanAbstractIn Vibrio cholerae, the lysogenic bacteriophage CTXΦ carries the cholera toxin genes ctxAB, which can be transferred from toxigenic strains to nontoxigenic strains through infection and lysogenic conversion of CTXΦ. This phage also has the precursor genome which does not...
Mon, 02/10/2020 - 04:09
Author(s): Rafaela C. Baptista, Heber Rodrigues, Anderson S. Sant'AnaAbstractThe present work aimed to evaluate the frequency of seafood consumption by the Brazilian population, the safety practices during seafood handling and preservation as well as to evaluate the consumers’ perception on the risks associated with these foods. A total of 962 respondents responded to a survey divided into three...
Fri, 02/07/2020 - 04:16
Author(s): Zhuoxing Zhang, Chao Zhang, Xiaoling Dai, Ruidong Zhang, Xueying Cao, Kaiqiang Wang, Xin Huang, Qian RenAbstractNuclear factor κB (NF-κB) plays a key role in the innate immunity of invertebrates. Relish belongs to the NF-κB family. In insects, alternative splicing induces the sequence diversity of the Relish gene. However, information on the roles of various relish isoforms in...
Fri, 02/07/2020 - 04:16
Author(s): Le Fei Jiao, Tian Meng Dai, Sun Qian Zhong, Min Jin, Peng Sun, Qi Cun ZhouAbstractThe intestine is the primary target of pathogenic microbes during invasion. However, the interaction of Vibrio parahaemolyticus (V. parahaemolyticus) with intestinal epithelial cells and its effects on the intestinal function of Litopenaeus vannamei (L. vannamei) are poorly studied. Therefore, the aim of...
Wed, 02/05/2020 - 03:27
The objective of this study was to determine whether seeds of Brassica oleracea var. italica (i.e. broccoli, an edible plant) produce defensins that inhibit phytopathogenic fungi and pathogenic bacteria of clinical significance. Crude extracts obtained from broccoli seeds were fractioned by molecular exclusion techniques and analyzed by liquid chromatography-high-resolution mass spectrometry. Two...
Wed, 02/05/2020 - 03:26
Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific...
Mon, 02/03/2020 - 03:34
Author(s): Yan-li Su, Guang-hua Wang, Jing-jing Wang, Bing Xie, Qin-qin Gu, Dong-fang Hao, Hong-mei Liu, Min ZhangAbstractAt present, several reports have indicated that the C-terminal peptides of tissue factor pathway inhibitor 1 (TFPI-1) were active antibacterial peptides. However, the functions of TFPI-1 C-terminal peptides in teleost are still very limited. In this study, a C-terminal peptide...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:25
Vibrio parahaemolyticus (Vp), a major food-borne pathogen, is responsible for severe infections such as gastroenteritis and septicemia, which may be accompanied by life-threatening complications. While studies have evaluated factors that affect the virulence of the pathogen, none have investigated the interaction of Vp with gut microbiota. To address this knowledge gap, we compared the effect of...
Thu, 01/30/2020 - 03:25
An estimated 700 000 soldiers belonging to the Union Army and the Confederate States Army died in the 1861–65 American Civil War.1 Many camp epidemics attributed to dysentery, typhoid fever, measles, smallpox, cholera, and malaria spread quickly among the troops, affecting the Confederate Army more and surely to some extent shaping the outcome of the war.2
Wed, 01/29/2020 - 04:01
Author(s): Nguyen Nhat Minh Phuong, Thien Trung Le, John Van Camp, Katleen RaesAbstractMicrobial safety in food products is not always adequately controlled. Chemical antimicrobials which are recognized as hazards to human health are gradually replaced by natural antimicrobial compounds. In the current study, the antimicrobial activity against some Gram-positive and Gram- negative bacteria by the...
Sun, 01/26/2020 - 03:27
Aim The current study was conducted to determine the incidence, antibiotic resistance and virulence genes of Vibrio strains isolated from ready‐to‐eat shrimps in Edo and Delta States, Nigeria. Methods and Results A total of 1440 ready‐to‐eat shrimp's samples were obtained from open markets from November 2016 to October 2017 and analysed using standard culture‐based procedures. Overall, our result...
Fri, 01/24/2020 - 03:33
Author(s): Junjie Nie, Zhixue Yu, Defu Yao, Fan Wang, Chunhua Zhu, Kaihui Sun, Jude Juventus Aweya, Yueling ZhangAbstractThe histone deacetylase, sirtuin 6 (SIRT6), plays an essential role in the regulation of oxidative stress, mitochondrial function and inflammation in mammals. However, the specific role of SIRT6 in invertebrate immunity has not been reported. Here, we characterized for the...
Fri, 01/24/2020 - 03:26
Populations of the bacterium Vibrio cholerae consist of dozens of distinct lineages, with primarily (but not exclusively) members of the pandemic generating (PG) lineage capable of causing the diarrheal disease cholera. Assessing composition and temporal dynamics of such populations requires extensive isolation efforts and thus only rarely covers large geographic areas or timeframes exhaustively...

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