Vibrio

Sat, 12/21/2019 - 04:05
Author(s): Le He, LiQun Wu, YiJun Tang, Peng Lin, ShaoWei Zhai, YiQun Xiao, SongLin GuoAbstractIn cultivated European eels, Aeromonas hydrophila, Edwardsiella anguillarum and Vibrio vulnificus are three important bacterial pathogens. In this study, an expressed recombinant Outer membrane proteinⅡ (rOmpⅡ) from A. hydrophila was intraperitoneally injected into European eels (Angullia angullia). All...
Fri, 12/20/2019 - 03:28
Vibrio parahaemolyticus is a leading cause of seafood‐borne bacterial gastroenteritis in humans. Since its discovery in 1950, this bacterium has been isolated in widespread outbreaks and in sporadic cases of gastroenteritis worldwide. Although the exotoxin, thermostable direct hemolysin (TDH), had been the focus of extensive research on the pathogenicity of V. parahaemolyticus, the whole‐genome...
Fri, 12/20/2019 - 03:26
Vibrio cholerae is a leading waterborne pathogenic bacterium worldwide. It can cause human cholera that is still pandemic in developing nations. Detection of V. cholerae contamination in drinking water and aquatic products is imperative for assuring food safety. In this study, a simple, sensitive, specific, and visualized method was developed based on loop-mediated isothermal amplification (LAMP...
Fri, 12/20/2019 - 03:26
ICEpMERPH, the first integrative conjugative element (ICE) of the SXT/R391 family isolated in the United Kingdom and Europe, was analyzed to determine the nature of its adaptive functions, its genetic structure, and its homology to related elements normally found in pathogenic Vibrio or Proteus species. Whole genome sequencing of Escherichia coli (E. coli) isolate K802 (which contains the...
Thu, 12/19/2019 - 03:29
Reduced digestibility of foods containing oxidized proteins and the subsequent excessive accumulation of undigested components in the colon may cause changes in the intestinal flora composition. This study evaluates the characteristics of this change and the potential adverse effects on organisms. Methods and results Pork was cooked using sous‐vide or at high temperature and pressure (HTP), and...
Wed, 12/18/2019 - 04:04
Author(s): Xiao-hong Sun, Li-ran Hao, Qing-chao Xie, Wei-qing Lan, Yong Zhao, Ying-jie Pan, Vivian C.H. WuAbstractThis study aimed to investigate the antimicrobial effect and membrane damage mechanism of blueberry extract against Vibrio parahaemolyticus. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of blueberry extract against three strains of V....
Mon, 12/16/2019 - 03:33
Author(s): Songlin Guo, Le He, Liqun Wu, Yiqun Xiao, Shaowei Zhai, Qinpi YanAbstractIn cultivated European eels, Aeromonas hydrophila, Edwardsiella anguillarum and Vibrio vulnificus are three important bacterial pathogens. In this study, European eels (Anguilla anguilla) were immunized by the bivalent expression products of the outer membrane protein (Omp) gene from A. hydrophila (OmpⅡ) and E....
Sun, 12/15/2019 - 03:36
Author(s): Yuying Sun, Congcong Yan, Mengfei Liu, Yujie Liu, Wenzheng Wang, Wei Cheng, Fusheng Yang, Jiquan ZhangAbstractDuring the immune defense reaction of invertebrate, a plenty of reactive oxygen species (ROS) could be induced to product. Though ROS can kill foreign invaders, the accumulation of these reactive molecules in animals will cause serious cell damage. Carotenoids could function as...
Sun, 12/15/2019 - 03:33
Author(s): Xuetong Li, Yuan Su, Huashuo Chu, Shuxia Lyu, Jingjing Tian, Wentao XuAbstractVibrio parahaemolyticus is a major hidden danger of food safety. To develop a rapid, sensitive and on-site detecting method of Vibrio parahaemolyticus (V. parahaemolyticus), a strand replacement primer thermostat phosphate (SRPP) visual sensor was proposed, based on Bst DNA polymerase and pyrophosphatase. The...
Fri, 12/13/2019 - 03:34
Cholera caused by the Gram-negative bacterium Vibrio cholerae still remains a major health burden in developing countries due to its high transmissibility and multidrug resistance. Alternative strategies are in quest to curtail the disease focusing on antivirulent approaches, such as biofilm inhibition, which make bacteria more susceptible to antibiotic therapies. The biofilm state is important...
Wed, 12/11/2019 - 03:50
Y. Tu et al. We investigated the epidemiology of cholera in Taiwan during 2002–2018. Vibrio cholerae sequence type (ST) 75 clone emerged in 2009 and has since become more prevalent than the ST69 clone from a previous pandemic. Closely related ST75 strains have emerged in 4 countries and may now be widespread in Asia.
Tue, 12/10/2019 - 03:38
Author(s): Qingchao Wang, Wei Ji, Zhen XuAbstractIn the past decades, the aquaculture industry made great progress in China, which contributes more than 70% yield of the world's farmed fish. Along with the rapid growth of fish production, increased emergence and outbreak of numbers of diseases pose harm to the aquaculture industry and food safety. From the efficient, safe, environmental and...
Tue, 12/10/2019 - 03:34
Author(s): Tao Lei, Jumei Zhang, Fufeng Jiang, Min He, Haiyan Zeng, Moutong Chen, Rui Pang, Haoming Wu, Shi Wu, Juan Wang, Yu Ding, Qingping WuAbstractThe aim of this study is to investigate the prevalence of integrons and integron-associated antibiotic resistance in V. parahaemolyticus strains collected from RTE foods in China, and to carry out a comprehensive analysis on the molecular...
Sat, 12/07/2019 - 03:26
The Cpx response is one of several envelope stress responses that monitor and maintain the integrity of the gram-negative bacterial envelope. While several conditions that are known or predicted to generate misfolded inner membrane proteins activate the Cpx response, the molecular nature of the Cpx inducing cue is not yet known. Studies have demonstrated that mutation of multidrug efflux pumps...
Wed, 12/04/2019 - 03:36
Author(s): Xiaolin Meng, Wenpan Hu, Shengkui Wu, Zhenxiang Zhu, Ronghua Lu, Guokun Yang, Chaobin Qin, Liping Yang, Guoxing NieAbstractThe Chinese yam peel (CYP) is a by-product of yam processing that is rich in various nutrients and a good source for feed additives. This study investigated the effects of CYP on the intestinal microbiota and gut defence barrier of the common carp (Cyprinus carpio...
Wed, 12/04/2019 - 03:36
Author(s): Bing-Chang Lee, Chun-Wei Hung, Cheng-Yung Lin, Chen-Han Shih, Huai-Jen TsaiAbstractTo develop an alternative to conventional antibiotics used in the aquaculture and livestock industries, we employed Bacillus subtilis, considered a biosafe microorganism, to express the degradable antimicrobial peptide lactoferricin. An expression plasmid pP43-6LFBII-GFP, in which reporter GFP cDNA was...
Wed, 12/04/2019 - 03:26
Vibrio parahaemolyticus is a naturally occurring bacterium in estuarine waters and is a major cause of seafood-borne illness. The bacterium has been consistently identified in Pacific Northwest waters and elevated illness rates of vibriosis in Washington State have raised concerns among growers, risk managers, and consumers of Pacific oysters (Crassostrea gigas). In order to better understand pre...
Wed, 12/04/2019 - 03:26
Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2...
Tue, 12/03/2019 - 03:46
Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle. Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology. PglLVc, a predicted oligosaccharyltransferase of V....
Mon, 12/02/2019 - 03:36
Author(s): Gaoyang Wang, Yutong Lei, Ting Kang, Zhi Li, Hui Fei, Shun Yang, Peng Zhou, Chunsheng Wang, Zhengbing Lv, Mengmeng Huang, Xuewei XuAbstractC-type lectins are Ca2+-dependent carbohydrate-binding proteins containing one or more carbohydrate-recognition domains (CRDs). C-type lectins play crucial roles in innate immunity, including nonself-recognition and pathogen elimination. In the...
Fri, 11/29/2019 - 03:26
Author(s): Tao Lei, Fufeng Jiang, Min He, Jumei Zhang, Haiyan Zeng, Moutong Chen, Rui Pang, Shi Wu, Lei Wei, Juan Wang, Yu Ding, Qingping WuAbstractVibrio parahaemolyticus is the leading cause of foodborne bacterial poisoning in China. The aim of this research is to conduct a study on the prevalence, virulence, and antimicrobial resistance of V. parahaemolyticus from different types of food...
Thu, 11/28/2019 - 04:14
ABSTRACT Vibrio cholerae is a noninvasive pathogen that colonizes the small intestine and produces cholera toxin, causing severe secretory diarrhea. Cholera results in long lasting immunity, and recent studies have improved our understanding of the antigenic repertoire of V. cholerae. Interactions between the host, V. cholerae, and the intestinal microbiome are now recognized as factors which...
Wed, 11/27/2019 - 03:39
ABSTRACT The 7th cholera pandemic began in 1961 in Sulawesi, Indonesia, and then spread around the world in at least three waves. However, the lack of genome sequences for Vibrio cholerae strains under long-term surveillance in East Asia, especially in China, has restricted our understanding of the dynamics of the intracountry and intercountry evolution and transmission of the 7th-pandemic clones...
Tue, 11/26/2019 - 03:25
Espinoza-Vergara et al. unveiled a novel transmission mode of Vibrio cholerae based on environmental protozoan predation, which the bacterial pathogen evades via its release in ‘expelled food vacuoles.’ Vacuole-enclosed bacteria are not only fairly protected against environmental stressors, but also show enhanced intestinal colonization fitness upon oral ingestion.
Mon, 11/25/2019 - 03:35
Author(s): Pramod Kumar, Priti Yadav, K.V. Ingole, Rishi Jaiswal, N.S. Khalid, Durgesh G. Deshmukh, A.K. Goel, P.K. YadavaAbstractIt is evident from previous cholera epidemic/outbreaks in India, Africa and America that seventh pandemic ElTor (7PET) with Haitian genotype (7PET-HCT) were associated with increased mortality. Present study highlights emergence of 7PET-HCT isolates causing two cholera...
Sat, 11/23/2019 - 03:36
Author(s): Els Vossen, Sophie Goethals, Jo De Vrieze, Nico Boon, Thomas van Hecke, Stefaan De SmetAbstractPigs were fed either red and processed meat or chicken meat within either a prudent or a Western dietary pattern for four weeks (2 × 2 full factorial design). The colon microbial community and volatile organic compounds were assessed (either quantified or based on their presence). Results...
Sat, 11/23/2019 - 03:28
Here, two monoclonal antibodies (MAbs) specific to different epitopes on ToxB, a toxin produced by Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VPAHPND), were employed to develop a rapid strip test. One MAb was conjugated to colloidal gold to bind to ToxB at the application pad, and another MAb was used to capture colloidal gold MAb–protein complexes at the test...
Sat, 11/23/2019 - 03:26
Extracellular vesicles secreted by Gram-negative bacteria have proven to be important in bacterial defense, communication and host–pathogen relationships. They resemble smaller versions of the bacterial mother cell, with similar contents of proteins, LPS, DNA, and RNA. Vesicles can elicit a protective immune response in a range of hosts, and as vaccine candidates, it is of interest to properly...
Fri, 11/22/2019 - 03:38
The emergence of multidrug-resistant bacterial strains in diverse settings has been reported globally. In the Philippine shrimp aquaculture industry, antibiotics are used for the treatment of bacterial diseases during the production cycle. We report the draft genome of Vibrio parahaemolyticus PH698, a multidrug-resistant strain isolated from a Philippine shrimp farm.
Fri, 11/22/2019 - 03:37
qnr genes are found in aquatic bacteria and were present in the bacterial community before the introduction of synthetic quinolones. Their natural functions are unknown. We evaluated expression of chromosomal qnr in Vibrio species in response to environmental stresses and DNA-damaging agents. Subinhibitory concentrations of quinolones, but not other DNA-damaging agents, increased expression of...
Thu, 11/21/2019 - 03:25
Cholera toxin B subunit (CTB), a non-toxic homopentameric component of Vibrio cholerae holotoxin, is an oral cholera vaccine antigen that induces an anti-toxin antibody response. Recently, we demonstrated that a recombinant CTB variant with a Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention motif (CTB-KDEL) exhibits colon mucosal healing effects that have therapeutic implications for...
Wed, 11/20/2019 - 03:44
All organisms depend on symbiotic associations with bacteria for their success, yet how these interspecific interactions influence the population structure, ecology, and evolution of microbial symbionts is not well understood. Additionally, patterns of genetic variation in interacting species can reveal ecological traits that are important to gene flow and co-evolution. In this study, we define...
Mon, 11/18/2019 - 03:36
Author(s): Di Wang, Shujing Zhang, Baozhong LiuAbstractTAF5L is a component of the P300/CBP-associated factor (PCAF) histone acetylase complex, which serves as a coactivator and takes part in basal transcription such as promoter recognition, complex assembly and transcription initiation. In our study, the full-length sequence of MpTAF5L was identified and characterized in the clam M. petechialis...
Mon, 11/18/2019 - 03:35
Author(s): Gao Xiaolong, Ke Caihuan, Wu Fucun, Li Xian, Liu YingAbstractTo study the effects of Bacillus lincheniformis feeding frequency on the survival and growth of Haliotis discus hannai abalone, we measured the expression levels of nonspecific immune genes and monitored the anti-Vibrio parahaemolyticus immune reaction. H. discus hannai (shell length: 32.75 ± 2.63 mm, body weight: 4.91 ± 0.34...
Sat, 11/16/2019 - 03:26
Apolipoprotein A-I is an anti-inflammatory, antioxidative, cardioprotective, anti-tumorigenic, and anti-diabetic in mammals. Apolipoprotein A-I also regulates innate immune defense mechanisms in vertebrates and invertebrates. Apolipoproteins A-I from mammals and several teleosts display antibacterial activities against Gram negative and Gram positive bacteria. The present study describes...
Sat, 11/16/2019 - 03:25
Unabsorbed proteins reach the colon and are fermented by the microbiota, yielding a variety of harmful metabolites. In the present study, a 16S rRNA gene survey identified the bacterial taxa flourishing in 11 batch fermentations with proteins and peptones as the sole fermentable substrates, inoculated with the feces of six healthy adults. Organic acids, ammonia, and indole resulting from protein...
Fri, 11/15/2019 - 03:36
We report the first draft genome sequence of an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strain isolated from a Penaeus vannamei sample from the Philippines. The strain carries the genes encoding the Pir-like toxin pair PirAvp and PirBvp.
Thu, 11/14/2019 - 03:25
The dynamic nature of Vibrio parahaemolyticus epidemiology has presented a unique challenge for disease intervention strategies. Despite the continued rise of disease incidence and outbreaks of vibriosis, as well as the global emergence of pandemic clones and serovariants with enhanced virulence, there is a paucity of molecular methods for the serotyping of V. parahaemolyticus strains to improve...
Wed, 11/13/2019 - 03:35
Author(s): Zhouyi Zhang, Weijia Zhang, Changkao Mu, Ronghua Li, Weiwei Song, Yangfang Ye, Ce Shi, Lei Liu, Huan Wang, Chunlin WangAbstractGalectins are a family of β-galactoside-binding lectins that play key roles in the invertebrate innate immunity system, but no galectin genes have been identified in the mud crab (Scylla paramamosain) so far. The present study is the first to clone a galectin...
Wed, 11/13/2019 - 03:25
The type VI secretion system (T6SS) is a lethal yet energetically costly weapon in gram-negative bacteria. Through contraction of a long sheath, the T6SS ejects a few copies of effectors accompanied by hundreds of structural carrier proteins per delivery. The few ejected effectors, however, dictate T6SS functions. It remains elusive...
Tue, 11/12/2019 - 03:37
The Na+ - ion translocating NADH:quinone oxidoreductase (NQR) from Vibrio cholerae is a membrane bound respiratory enzyme which harbors flavins and Fe-S clusters as redox centers. The NQR is the main producer of sodium motive force (SMF) and drives energy-dissipating processes such as flagellar rotation, substrate uptake, ATP synthesis and cation-proton antiport. The NQR requires for its...
Tue, 11/12/2019 - 03:29
The Pacific white shrimp Penaeus vannamei is cultivated worldwide and is an important economic resource in Mexico. Despite continuous development of shrimp aquaculture in Mexico, this activity has exhibited variable production rates because of health‐related problems. Most of the health problems have been caused by viruses, such as IHHNV [syn = penstyldensovirus (PstDV1)], TSV, and WSSV, and...
Thu, 11/07/2019 - 03:26
Toxigenic Vibrio cholerae strains, including strains in serogroups O1 and O139 associated with the clinical disease cholera, are ubiquitous in aquatic reservoirs, including fresh, estuarine, and marine environments. Humans acquire cholera by consuming water and/or food contaminated with the microorganism. The genome of toxigenic V. cholerae harbors a cholera-toxin producing prophage (CT-prophage...
Wed, 11/06/2019 - 03:36
Author(s): Zhuoxing Zhang, Keke Han, Xiaoling Dai, Ruidong Zhang, Xueying Cao, Chao Zhang, Kaiqiang Wang, Xin Huang, Qian RenAbstractTwo lipopolysaccharides (LPS) and β-1, 3-glucan binding protein (LGBP), designated as PcLGBP isoform1 and PcLGBP isoform2, respectively, were identified from Procambarus clarkii in this study. The full-length cDNA of PcLGBP isoform1 was 1308 bp containing an open...
Wed, 11/06/2019 - 03:27
Vibrio parahemolyticus is a halophilic bacterium which causes widespread seafood poisoning pathogenicity. Although the incidence of disease caused by V. parahemolyticus was stepwise increased, the pathogenic mechanism remained unclear. Herein, the difference of V. parahemolyticus’s metabonomic which on blood agar and seawater beef extract peptone medium was detected via nuclear magnetic resonance...
Wed, 11/06/2019 - 03:27
ABSTRACT The Malaysian and global shrimp aquaculture production has been significantly impacted by acute hepatopancreatic necrosis disease (AHPND) typically caused by Vibrio parahaemolyticus harboring the pVA plasmid containing the pirAVp and pirBVp genes, which code for Photorhabdus insect-related (Pir) toxin. The limited genomic resource for V. parahaemolyticus strains from Malaysian...
Mon, 11/04/2019 - 03:35
Author(s): A.S. Gladkikh, S.I. Feranchuk, A.S. Ponomareva, N.O. Bochalgin, L.V. MironovaАbstractCurrently, the spread of antimicrobial resistance (AMR) is a global trend and poses a severe threat to public health. The causative agent of cholera, an extremely virulent disease with pandemic expansion, becomes more and more resistant to a wider range of drugs with every coming year. The Vibrio...

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