Vibrio

Sun, 11/03/2019 - 02:36
Author(s): Ying Huang, Qian RenAbstractKruppel-like factors (KLFs) belong to a family of zinc finger-containing transcription factors that are widely present in eukaryotes. In the present study, a novel KLF from the giant river prawn Macrobrachium rosenbergii (designated as MrKLF) was successfully cloned and characterized. The full-length cDNA of MrKLF was 1799 bp with an open reading frame of...
Sun, 11/03/2019 - 02:35
Author(s): Lichao Zhao, Xinrui Lv, Xiao Cao, Jingfeng Zhang, Xiaokui Gu, Haiyan Zeng, Li WangAbstractImmunomagnetic separation (IMS) is an effective method for specific enrichment and purification of target food-borne pathogens from complex food samples. To detect viable but non-culturable (VBNC) Vibrio parahaemolyticus (V. parahaemolyticus) with greater accuracy and sensitivity, we used an...
Thu, 10/31/2019 - 03:37
To maintain the turgor pressure of the cell under high osmolarity, bacteria accumulate small organic compounds called compatible solutes, either through uptake or biosynthesis. Vibrio parahaemolyticus, a marine halophile and an important human and shellfish pathogen, has to adapt to abiotic stresses such as changing salinity. Vibrio parahaemolyticus contains multiple compatible solute...
Thu, 10/31/2019 - 03:35
Author(s): Xiaoying Zheng, Jialu Guo, Huishan Rao, Du Guo, Yixiao Huang, Yunfeng Xu, Sen Liang, Xiaodong Xia, Chao ShiAbstractCoenzyme Q0 (CoQ0) demonstrates antineoplastic and anti-inflammatory properties; however, its antimicrobial and antibiofilm activities against Vibrio parahaemolyticus have not been extensively examined. Thus, the aim of the current study was to investigate the...
Wed, 10/30/2019 - 03:38
ABSTRACT Swarmer cells of the Gram-negative uropathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10 to 100 μm) and multinucleate during their growth and motility on polymer surfaces. We demonstrated that the increasing cell length is accompanied by a large increase in flexibility. Using a microfluidic assay to measure single-cell mechanics, we identified large...
Sun, 10/27/2019 - 03:49
Author(s): Qiying Wang, Hao Li, Kaimin Zhou, Xiang Qin, Qun Wang, Weiwei LiAbstractThe Rab family is the most significant subfamily of small GTP-binding proteins. These proteins have widespread intracellular localization and play an important role in many biological processes. Rab7 plays a crucial role in the innate immune system of crustaceans. In the present study, we cloned and characterized...
Sun, 10/27/2019 - 03:44
Author(s): David C. Love, Lillian M. Kuehl, Robert M. Lane, Jillian P. Fry, Jamie Harding, Benjamin J.K. Davis, Kate Clancy, Bobbi HudsonAbstractVibrio bacteria can accumulate in molluscan shellfish and cause human diseases. The United States (U.S.) has implemented Vibrio Control Plans to mitigate risks associated with these bacteria, which include time and temperature requirements for post-...
Sun, 10/27/2019 - 03:43
Author(s): Yuxuan Zhang, Jian Lu, Jun Wu, Jianhua Wang, Yongming LuoAbstractMicroplastics have become emerging pollutants and served as potential vectors for harmful bacteria, while rare information on the emergency and propagation of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) on the surface of microplastics is available. This study investigated the enrichment of...
Sat, 10/26/2019 - 03:50
by Po-Yao Chuang, Tien-Yu Yang, Tsan-Wen Huang, Yao-Hung Tsai, Kuo-Chin Huang, Hsu-Huei Weng Background Vibrio vulnificus necrotizing skin and soft tissue infections (VNSSTIs) are associated with a high mortality rate that varies remarkably with host susceptibility. Hepatic disease (HD) is considered the key risk factor for high VNSSTIs incidence and mortality; however, there is limited evidence...
Sat, 10/26/2019 - 03:35
Streptococcus agalactiae is a major pathogen causing streptococcosis. To prevent and control this bacterial disease, antagonistic bacteria have become a new research hotspot. This study evaluated the probiotic potential of Bacillus velezensis LF01 strain, which is antagonistic to S. agalactiae. The active compounds produced by LF01 showed antimicrobial activity against a broad spectrum of fish...
Thu, 10/24/2019 - 03:44
Author(s): Marisol Goni-Urriza, Christophe Klopp, Magali Ranchou-Peyruse, Anthony Ranchou-Peyruse, Mathilde Monperrus, Bahia Khalfaoui-Hassani, Rémy GuyoneaudAbstractMercury methylation converts inorganic mercury into the toxic methylmercury, and the consequences of this transformation are worrisome for human health and the environment. This process is performed by anaerobic microorganisms, such...
Sun, 10/20/2019 - 03:42
Author(s): Dewei Cheng, Hongxing Liu, Hongkuan Zhang, Tan Kar Soon, Ting Ye, Shengkang Li, Hongyu Ma, Huaiping ZhengAbstractHeat shock proteins (HSPs) are a family of conserved proteins that enhance stress resistance and protect cells from external damage. In the present study, the full-length HSP70 cDNA from the noble scallop Chlamys nobilis (designated CnHSP70) was first cloned and...
Sun, 10/20/2019 - 03:39
Author(s): Caroline D'Souza, Ballamoole Krishna Kumar, Praveen Rai, Vijay Kumar Deekshit, Indrani KarunasagarAbstractA SYBR green based qPCR assay targeting a unique region of gyrB was developed for the detection of Vibrio vulnificus. The specificity of the assay was studied using V. vulnificus and other bacterial strains belonging to Vibrio and non-Vibrio species. The assay unambiguously...
Thu, 10/17/2019 - 03:32
This research aimed to determine microbiological and chemical quality of different types of salted pearl mullet (Chalcalburnus tarichi Pallas, 1811). Samples of salted pearl mullet (C. tarichi Pallas, 1811), produced by six different methods, were obtained from three different local producers at the end of the hunting season of 2016 and 2017 (May–June) with wooden boxes, plastic cans, or in...
Thu, 10/17/2019 - 03:32
Masmin preparation is a traditional method that has been practiced for many years to preserve skipjack tuna (Katsuwonus pelamis) mainly in Lakshadweep. However, the method is not evaluated scientifically for its quality concerning nutrition and environmental contamination. Thus, in the present study tuna fillets from four stages of Masmin preparation, namely fresh, boiling, smoking, and sun‐...
Wed, 10/16/2019 - 03:32
Cobamides are a family of cofactors that are widely‐utilized by diverse organisms, and there are over a dozen variants, including vitamin B12. V. cholerae imports a range of cobamides, but it cannot utilize a variant called pseudocobalamin and instead remodels it through a distinct pathway. Abstract Cobamides are a group of compounds including vitamin B12 that can vary at the lower base position...
Tue, 10/15/2019 - 03:47
Author(s): Elumalai Preetham, Abdul Salam Rubeena, Baskaralingam Vaseeharan, Mukesh Kumar Chaurasia, Jesu Arockiaraj, Rolf Erik OlsenAbstractThe study is carried out to understand the antimicrobial and immunological response of a potential immune molecule lectin, MmLec isolated from haemolymph of Speckled shrimp, Metapenaeus monoceros. MmLec was purified using mannose coupled Sepharose CL-4B...
Sun, 10/13/2019 - 03:43
Author(s): Yu-Lei Chen, Keke Han, Xin Huang, Zhuoxing Zhang, Xihe Wan, Qian RenAbstractThe caspase is an essential module in the Drosophila immune deficiency (IMD) pathway, which plays a crucial role in countering pathogen infection. In this study, a gene named PcCaspase-3C was found in Procambarus clarkia with a full-length of 4684 bp, including a 1572 bp opening reading frame, which encoded a...
Sat, 10/12/2019 - 03:32
The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n=4), V. alginolyticus (n=11), V. diabolicus (n=14), and V. harveyi (n=3) were detected using gyrB sequencing. The phenotypic pathogenicity revealed DNase, amylase...
Fri, 10/11/2019 - 03:32
The present study assessed the occurrence, virulence determinants and antimicrobial susceptibility patterns of Vibrio spp. isolated from live Manila clam (Ruditapes philippinarum). A total of 31 Vibrio spp. including 27 V. diabolicus, 2 V. fluvialis, 1 V. alginolyticus, and 1 V. antiquarius were isolated and identified. Phenotypic detection of DNase, lipase, phospholipase, amylase, and caseinase...
Sat, 10/05/2019 - 03:39
Author(s): Gao Xiaolong, Ke Caihuan, Zhang Mo, Li Xian, Wu Fucun, Liu YingAbstractThe effects of a diet containing the probiotic Bacillus amyloliquefaciens on the survival and growth of Haliotis discus hannai were evaluated by measuring growth and hematological parameters and the expression levels of nonspecific immune genes. In addition, the abalone's response to Vibrio parahaemolyticus...
Thu, 10/03/2019 - 03:32
Doctor fish (Garra rufa, Heckel, 1843) are increasingly used for cosmetic treatment raising particular concerns regarding the potential transmission of infections to clients. Investigations of microbial causes undertaken in two outbreaks of mortality among G. rufa used for cosmetic treatment revealed the presence of multiple bacteria, including both fish and human pathogens such as Aeromonas...
Wed, 10/02/2019 - 03:42
by Iftekhar Bin Naser, M. Mozammel Hoque, Shah Nayeem Faruque, M. Kamruzzaman, Shinji Yamasaki, Shah M. Faruque Background Toxigenic Vibrio cholerae resides in aquatic reservoirs of cholera-endemic areas mostly in a dormant form known as conditionally viable environmental cells (CVEC) in which the bacteria remain embedded in an exopolysaccharide matrix, and fail to grow in routine bacteriological...
Mon, 09/30/2019 - 16:54
ABSTRACT We have previously identified Vibrio cholerae mutants in which the stress response to subinhibitory concentrations of aminoglycoside is altered. One gene identified, VC1636, encodes a putative DNA/RNA helicase, recently named RadD in Escherichia coli. Here we combined extensive genetic characterization and high-throughput approaches in order to identify partners and molecular mechanisms...
Mon, 09/30/2019 - 16:54
ABSTRACT BolA family proteins are conserved in Gram-negative bacteria and many eukaryotes. While diverse cellular phenotypes have been linked to this protein family, the molecular pathways through which these proteins mediate their effects are not well described. Here, we investigated the roles of BolA family proteins in Vibrio cholerae, the cholera pathogen. Like Escherichia coli, V. cholerae...
Mon, 09/30/2019 - 16:54
ABSTRACT The pathogenic bacterium Vibrio vulnificus exhibits the ability to form biofilm, for which initiation is dependent upon swimming motility by virtue of a polar flagellum. The filament of its flagellum is composed of multiple flagellin subunits, FlaA, -B, -C, and -D. In V. vulnificus genomes, however, open reading frames (ORFs) annotated by FlaE and -F are also present. Although neither...
Sun, 09/29/2019 - 03:31
Author(s): Chao Peng, Dongchang Xie, Chao Zhao, Haidong Xu, Sigang Fan, Lulu Yan, Pengfei Wang, Lihua QiuAbstractAkirin, which are members of the NF-κB signaling pathway, play critical roles in regulating the expression of antimicrobial peptides. In the present study, the Akirin gene from Penaeus monodon was identified from a transcriptome database and designated as PmAkirin. The complete...
Sun, 09/29/2019 - 03:31
Author(s): Xiaoxu Liu, Weiye Li, Lihua Jiang, Zhenming Lü, Minhai Liu, Li Gong, Bingjian Liu, Liqin Liu, Xiaolong YinAbstractLong non-coding RNA refers to an RNA transcript of a non-coding protein with a sequence length greater than 200 bp. More and more reports indicated that lncRNA was involved in the regulation of gene expression as a signalling molecule, an inducing molecule, a leader...
Sun, 09/29/2019 - 03:31
Author(s): Qian Feng, Yueqian Huang, Defu Yao, Chunhua Zhu, Shengkang Li, Hongyu Ma, Jude Juventus Aweya, Yueling ZhangAbstractProtein kinase CK2 (CK2) is a ubiquitous serine/threonine kinase with multiple cellular functions in vertebrates including apoptosis, differentiation, proliferation, survival, tumorigenesis, signal transduction, immune regulation and inflammation. In the current study,...
Sun, 09/29/2019 - 03:31
Author(s): Ruixia Wang, Xiucai Hu, Aijun Lü, Rongrong Liu, Jingfeng Sun, Yeong Yik Sung, Yajiao SongAbstractSkin plays an important role in the innate immune responses of fish, particularly towards bacterial infection. To understand the molecular mechanism of mucosal immunity of fish during bacterial challenge, a de novo transcriptome assembly of crucian carp Carassius auratus skin upon Aeromonas...
Tue, 09/24/2019 - 03:41
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Tue, 09/24/2019 - 03:41
Cyclic di-GMP (c-di-GMP) is a bacterial second messenger molecule that is important in the biology of Vibrio cholerae, but the molecular mechanisms by which this molecule regulates downstream phenotypes have not been fully characterized. We have previously shown that the Vc2 c-di-GMP-binding riboswitch, encoded upstream of the gene tfoY, functions as an off-switch in response to c-di-GMP. However...
Tue, 09/24/2019 - 03:40
Bacteria synthesize amino acids according to their availability in the environment or, in the case of pathogens, within the host. We explored the regulation of the biosynthesis of branched-chain amino acids (BCAAs) (l-leucine, l-valine, and l-isoleucine) in Vibrio alginolyticus, a marine fish and shellfish pathogen and an emerging opportunistic human pathogen. In this species, the ilvGMEDA operon...
Tue, 09/24/2019 - 03:40
The mortality rate associated with Vibrio vulnificus sepsis remains high. An in vitro time-kill assay revealed synergism between tigecycline and ciprofloxacin. The survival rate was significantly higher in mice treated with tigecycline plus ciprofloxacin than in mice treated with cefotaxime plus minocycline. Thus, combination treatment with tigecycline-ciprofloxacin may be an effective novel...
Tue, 09/24/2019 - 03:38
Author(s): Xiaoye Shen, Yi-Cheng Su, Chengchu Liu, Tom Oscar, Angelo DePaola
Tue, 09/24/2019 - 03:37
Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz BanaeiAbstractThough the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for...
Tue, 09/24/2019 - 03:27
Nitric oxide (NO) and its derivatives are important effectors of host innate immunity, disrupting cellular function of infecting pathogens. Transcriptome analysis of Vibrio vulnificus, an opportunistic human pathogen, identified a set of genes induced upon exposure to NO. Among them, VvhmpA (V. vulnificus hmpA), encoding a multidomain NO dioxygenase, was the most greatly induced upon exposure to...
Tue, 09/24/2019 - 03:27
The emerging threat of drug resistant bacteria has prompted the investigation into bacterial signaling pathways responsible for pathogenesis. One such mechanism by which bacteria regulate their physiology during infection of a host is through a process known as quorum sensing (QS). Bacteria use QS to regulate community-wide gene expression in response to changes in population density. In order to...
Mon, 09/23/2019 - 03:31
Author(s): Dinglong Yang, Yijing Han, Lizhu Chen, Ruiwen Cao, Qing Wang, Zhijun Dong, Hui Liu, Xiaoli Zhang, Qianqian Zhang, Jianmin ZhaoAbstractBactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was...
Mon, 09/23/2019 - 03:31
Author(s): Xianyun Ren, Yunbin Zhang, Ping Liu, Jian LiAbstractThis study aimed to use isobaric tags (IBTs) to investigate the immune response of the hepatopancreas of Marsupenaeus japonicas infected with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Liquid chromatography-tandem mass spectrometry and protein sequencing identified 1005 proteins. Among them, 109 proteins were...
Mon, 09/23/2019 - 03:30
Author(s): Haibo Zhou, Ying Liang, Lu Gao, Jianluan Ren, Feng Xue, Dehua Guo, Yuan Jiang, Zhenquan Yang, Lele Lian, Jianjun DaiAbstractQuinolone-resistant foodborne pathogens have become an important public health concern, however, little is known about the molecular mechanism of ciprofloxacin (CIP) resistance among Vibrio parahaemolyticus isolates. This study aimed to explore new genes...
Mon, 09/23/2019 - 03:29
Author(s): Aditi Gangopadhyay, Hirak Jyoti Chakraborty, Abhijit DattaAbstractCholera is a major global threat, affecting millions each year. The ADP ribosyltransferase activity of the active cholera toxin catalyses the massive loss of water and electrolytes during cholera infections. The active toxin heterodimer comprises the A1 subunit from Vibrio cholerae and ARF (ADP Ribosylation Factor) from...
Sun, 09/22/2019 - 03:31
Antibiotic tolerance, the ability to temporarily sustain viability in the presence of bactericidal antibiotics, constitutes an understudied and yet potentially widespread cause of antibiotic treatment failure. We have previously shown that the Gram-negative pathogen Vibrio cholerae can tolerate exposure to the typically bactericidal β-lactam antibiotics by assuming a spherical morphotype...
Sun, 09/22/2019 - 03:30
Author(s): Rasheduzzaman Rashu, Taufiqur Rahman Bhuiyan, Mohammad Rubel Hoq, Lazina Hossain, Anik Paul, Ashraful Islam Khan, Fahima Chowdhury, Jason B. Harris, Edward T. Ryan, Stephen B. Calderwood, Ana A. Weil, Firdausi QadriAbstractVibrio cholerae O1 can cause life threatening diarrheal disease if left untreated. T cells can play critical roles in inducing B cell mediated immunity. As the...
Sun, 09/22/2019 - 03:30
Author(s): Priyadarshini Mukherjee, Vivek Mondal, Ushasi Bhaumik, Ritam Sinha, Sounak Sarkar, Soma Mitra, Debaki Ranjan Howlader, Suhrid Maiti, Asish Kumar Mukhopadhyay, Shanta Dutta, Hemanta KoleyAbstractMultiple diarrheagenic enteric bacterial infections cause global morbidity and mortality. A combination vaccine is needed to combat different diarrhea-causing organisms. In our present work, we...
Sun, 09/22/2019 - 03:30
Author(s): Jing Li, Zhi-Bin Wu, Zhao Zhang, Ji-Wei Zha, Shen-Ye Qu, Xiao-Zhou Qi, Gao-Xue Wang, Fei LingAbstractNowadays, there is no suitable treatment for vibriosis in groupers. So an eco-efficient and environmentally friendly treatment is necessary for the grouper industry. Probiotic-feeding has been a promising strategy to control the bacterial pathogens in aquaculture. A new Bacillus...

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