Vibrio

Sun, 09/22/2019 - 03:30
Author(s): Phennapa Promthale, Pattira Pongtippatee, Boonsirm Withyachumnarnkul, Kanokpan WongprasertAbstractFishmeal is the main source of protein in the shrimp feed industry and is normally derived from trash fish. As such, the production of fishmeal has an adverse effect on the marine environment by taking away small and juvenile fish, leading to depletion of marine species. There is a need...
Sun, 09/22/2019 - 03:30
Author(s): Mengting Luo, Linwei Yang, Zi-ang Wang, Hongliang Zuo, Shaoping Weng, Jianguo He, Xiaopeng XuAbstractC-type lectins (CTLs) are a group of lectins with at least one carbohydrate recognition domain (CRD), the binding of which to carbohydrates requires the presence of calcium ions. CTLs generally function as pattern recognition receptors (PRRs), essentially participating in innate...
Sun, 09/22/2019 - 03:30
Author(s): Jiquan Zhang, Yujie Liu, Yongzhao Zhou, Wenzheng Wang, Naike Su, Yuying SunAbstractTrehalose, a nonreducing disaccharide, is present in a wide variety of organisms and plays a key role in many organisms under different stress conditions. In the study, the full-length cDNA sequence encoding trehalose-6-phosphate synthase (EcTPS) was obtained from Exopalaemon carinicauda. The complete...
Sun, 09/22/2019 - 03:30
Author(s): Jiajia Yu, Hongxia Wang, Xin Yue, Baozhong LiuAbstractMeretrix petechialis is an important commercial aquaculture species in China. During the clam culture period, mass mortality events often occurred due to the Vibrio infection. In this paper, M. petechialis were challenged with Vibrio parahaemolyticus immersion to simulate a natural infection, and the infection process were divided...
Sun, 09/22/2019 - 03:30
Author(s): Jianning Zhang, Yifei Zhang, Linbao Zhang, Qianyu Wei, Xiaoli Liu, Dinglong YangAbstractIn the present study, a sialic acid-binding lectin was cloned and characterized from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading frame of RpSabl encoded a polypeptide of 162 amino acids with a calculated molecular mass of 17.7 kDa. Analysis of the conserved domain...
Sun, 09/22/2019 - 03:30
Author(s): Werasan Kewcharoen, Prapansak SrisapoomeAbstractAcute hepatopancreatic necrosis disease (AHPND), a serious disease caused by some Vibrio spp., impacts the Pacific white shrimp industry worldwide, especially in Thailand. To effectively overcome this problem, efficacious probiotic candidates were isolated from shrimp farms near coastal areas. The isolated Bacillus probiotics were...
Sun, 09/22/2019 - 03:30
Author(s): Jianning Zhang, Yifei Zhang, Lizhu Chen, Jianmin Yang, Qianyu Wei, Bowen Yang, Xiaoli Liu, Dinglong YangAbstractIn the study, two c-type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin...
Sun, 09/22/2019 - 03:30
Author(s): Crystal Guluarte, Martha Reyes-Becerril, Daniel Gonzalez-Silvera, Alberto Cuesta, Carlos Angulo, María Ángeles EstebanAbstractThe aim of this study was to analyze the probiotic potential, fatty acid composition and immunostimulant activities of Kluyveromyces lactis M3 isolated from a hypersaline sediment. For this purpose, K. lactis M3 resistance to different pH, salinities and bile,...
Sun, 09/22/2019 - 03:30
Author(s): Ming Li, Chunxia Ma, Peng Zhu, Yanhao Yang, Aiyingi Lei, Xiaohan Chen, Wanwen Liang, Ming Chen, Jianha Xiong, Chaozheng LiAbstractCrustin is an antimicrobial peptide (AMP) that plays a key role in the innate immunity of crustaceans. This study cloned a new crustin from Pacific white shrimp Litopenaeus vannamei, which we designated as LvCrustinB, using rapid amplification of cDNA ends (...
Sun, 09/22/2019 - 03:30
Author(s): Shuang Zhang, Chaozheng Li, Wei Wang, Chenggui Wang, Chengbo Sun, Siuming Chan, Lili ShiAbstractProtein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals and Drosophila, but the function of PIAS in crustaceans is still...
Sun, 09/22/2019 - 03:26
The phenotypic characteristics and growth kinetics at several temperatures, salinities, and pH values of three Vibrio parahaemolyticus (Vp) strains with different virulence and one nonpathogenic strain were evaluated. Independent of the virulence of the strain, a high metabolic diversity was found, which yielded different colored phenotypes on the CHROMagar™ Vibrio. All strains were resistant to...
Sun, 09/22/2019 - 03:26
Acute hepatopancreatic necrosis disease (AHPND) causes massive mortality in shrimp ponds within the first month poststocking. The causative agent is a specific strain of Vibrio parahaemolyticus (VPAHPND) that has acquired the capability to produce virulent binary toxins called ToxA and ToxB. This study aims to test the effect of the addition of an autoinducer‐2‐containing cell‐free supernatant (...
Sun, 09/22/2019 - 03:26
In this study, the antibacterial properties of Pimpinella anisum essential oil (PAO) were evaluated against six food‐related bacteria. Afterward, the effects of different concentrations of PAO on microbial, chemical, and organoleptic properties of minced beef were evaluated during refrigerated storage. Based on the findings, PAO was effective against all of the tested strains, where minimum...
Sun, 09/22/2019 - 03:24
Aims:  To develop simple and rapid PCR‐fingerprinting methods for Vibrio cholerae O1 (El Tor and classical biotypes) and O139 serogroup strains which cause major cholera epidemics, on the basis of the diversity of superintegron (SI) carried by these strains. Methods and Results:  PCR‐restriction fragment length polymorphism (PCR‐RFLP) assay was developed targeting region between integrase gene in...
Sun, 09/22/2019 - 03:24
In the chicken slices and canned beef B.bacteriovorax induced a E.coli reduction equal to 4.3 and 2.1 logs, respectively, at 6 hours from contamination.♦ E. coli control; ● E. coli test; ▲ Bb control; ■ Bb test. Summary Bdellovibrio bacteriovorus is a predator micro‐organism towards other Gram‐negative bacteria. We tested B. bacteriovorus to control Escherichia coli growth on chicken slices and...
Sun, 09/22/2019 - 03:24
Kenya is endemic for cholera with different waves of outbreaks having been documented since 1971. In recent years, new variants of Vibrio cholerae O1 have emerged and have replaced most of the traditional El Tor biotype globally. These strains also appear to have increased virulence, and it is important to describe and document their phenotypic and genotypic traits. This study characterized 146 V...
Sun, 09/22/2019 - 03:22
This paper aims to characterize halophilic bacteria inhabiting Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria). In addition, screening of enzymatic activities, heavy metal tolerance and antagonistic potential against phytopathogenic fungi were tested. A total of 74 bacterial isolates were screened and phylogenetically...
Sun, 09/22/2019 - 03:22
Background The ability to identify bacterial pathogens that necessitate specific clinical management or public health action in children with acute gastroenteritis is crucial to patient care and public health. However, existing stool-testing guidelines offer inconsistent recommendations, and their performance characteristics are unknown. We evaluated 6 leading gastroenteritis guidelines (eg,...
Thu, 09/19/2019 - 14:29
Available online 13 September 2019 Author(s): Nan Ling, Jinling Shen, Jingjing Guo, Dexin Zeng, Jianluan Ren, Lixin Sun, Yuan Jiang, Feng Xue, Jianjun Dai, Baoguang LiAbstractVibrio parahaemolyticus is an important human pathogen causing a variety of life-threatening diseases and is widely distributed in marine and estuarine environments. The objective of this study was to develop a sensitive,...
Tue, 09/17/2019 - 20:00
Nitric oxide (NO) plays a major role in the regulation of mammalian biological functions. In recent years, NO has also been implicated in bacterial life cycles, including in the regulation of biofilm formation, and the metabolism of the bacterial second messenger signaling molecule cyclic-di-GMP. In a previous study, we reported the discovery of an NO-responsive quorum sensing (QS) circuit in...
Tue, 09/17/2019 - 12:01
MICROBIOLOGY Correction to Supporting Information for “Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi,” by Florence Caro, Nicole M. Place, and John J. Mekalanos, which was first published August 1, 2019; 10.1073/pnas.1906158116 (Proc. Natl. Acad. Sci. U.S.A. 116, 17013–17022). The authors note that Fig. S14 in the SI...
Tue, 09/17/2019 - 11:00
Bacteria synthesize amino acids according to their availability in the environment or, in the case of pathogens, within the host. We explored the regulation of the biosynthesis of branched-chain amino acids (BCAAs) (l-leucine, l-valine, and l-isoleucine) in Vibrio alginolyticus, a marine fish and shellfish pathogen and an emerging opportunistic human pathogen. In this species, the ilvGMEDA operon...
Fri, 09/13/2019 - 20:00
Recent evidence suggests that the commensal microbes act as a barrier against invading pathogens and enteric infections are the consequences of multi-layered interactions among commensals, pathogens, and the h...
Wed, 09/11/2019 - 00:00
Vibrio vulnificus is a Gram-negative naturally occurring bacterium found commonly in warm (>15oC), low-salinity/brackish waters worldwide. This bacterium occurs in high numbers in seafood, such as shellfish, particularly in warmer months. V. vulnificus is an important opportunistic pathogen that can cause both wound infections and sepsis, typically via exposure to seawater or through...
Mon, 09/09/2019 - 20:00
The Gram-negative bacterium Vibrio cholerae encodes two nucleases, Dns and Xds, which play a major role during the human pathogen’s lifecycle. Dns and Xds control three-dimensional biofilm formation and bacterial detachment from biofilms via degradation of extracellular DNA and thus contribute to the environmental, inter-epidemic persistence of the pathogen. During intestinal colonization the...
Thu, 09/05/2019 - 20:00
The self-transferring integrative and conjugative elements (ICEs) are large genomic segments carrying several bacterial adaptive functions including antimicrobial resistance (AMR). SXT/R391 family is one of the ICEs extensively studied in cholera-causing pathogen Vibrio cholerae. The genetic characteristics of ICE-SXT/R391 in V. cholerae are dynamic and region-specific. These ICEs in V. cholerae...
Mon, 09/02/2019 - 05:45
Abstract The mechanisms of inactivation against Gram‐negative bacteria (GNB) including Pseudomonas aeruginosa, Escherichia coli, and Vibrio parahaemolyticus, and Gram‐positive bacteria (GPB) involving Listeria monocytogenes and Staphylococcus aureus, by dielectric barrier discharge cold atmospheric plasma were elucidated. Both GNB and GPB were exposed to treatment with cold plasma produced from...
Mon, 09/02/2019 - 01:03
Available online 1 September 2019 Author(s): Yu Pang, Xi Guo, Xiao Tian, Fenxia Liu, Lu Wang, Junli Wu, Si Zhang, Shujie Li, Bin LiuAbstractVibrio parahaemolyticus is a major food-borne pathogen. V. parahaemolyticus infections are associated with various serotypes; to date, 71 K-serogroups of V. parahaemolyticus have been determined based on capsular polysaccharide (CPS) diversity. In this study...
Fri, 08/30/2019 - 07:34
, Volume 14, Issue 12, Page 1043-1053, August 2019.
Thu, 08/29/2019 - 11:00
Vibrio cholerae is a Gram-negative bacterial pathogen that causes the disease cholera, which affects nearly 1 million people each year. In between outbreaks, V. cholerae resides in fresh and salt water environments, where it is able to persist through changes in temperature, oxygen, and salinity. One key characteristic that promotes environmental persistence of V. cholerae is the ability to form...
Mon, 08/26/2019 - 01:05
Available online 24 August 2019 Author(s): Julio C. Ayala, Jorge A. Benitez, Anisia J. SilvaAbstractWe describe a proteomic approach to identify transcription factors binding to a target promoter. The method's usefulness was tested by identifying proteins binding to the Vibrio cholerae rpoS promoter in response to cell density. Proteins identified in this screen included the nucleoid-associated...
Thu, 08/22/2019 - 17:00
Sivan Laviad-Shitrit, Ido Izhaki, Malka Halpern Vibrio cholerae is the causative agent of cholera, a life-threatening diarrheal disease. Cholera causes epidemics and pandemics, but the ways this disease spreads worldwide is still unclear. This review highlights a relatively new hypothesis regarding the way V. cholerae can be globally dispersed. Copepods and chironomids are natural reservoirs of...
Thu, 08/22/2019 - 17:00
Tra-My Duong-Nu, Kwangjoon Jeong, Seol Hee Hong, Sao Puth, Soo Young Kim, Wenzhi Tan, Kwang Ho Lee, Shee Eun Lee, Joon Haeng Rhee The tad operons encode the machinery required for adhesive Flp (fimbrial low-molecular-weight protein) pili biogenesis. Vibrio vulnificus, an opportunistic pathogen, harbors three distinct tad loci. Among them, only tad1 locus was highly upregulated in in vivo growing...
Thu, 08/22/2019 - 04:23
Summary Bdellovibrio bacteriovorus is a predator microorganism towards other Gram‐negative bacteria. We tested B.bacteriovorus to control E.coli growth on chicken slices and canned beef. Moreover, we analyzed B. bacteriovorus lytic ability on 8 toxigenic or multidrug resistant E.coli strains. In chicken slices the predator induced the highest prey reduction (4.3 log) respect to control at 6 h. In...
Tue, 08/20/2019 - 17:00
Sean Giacomucci, Candice Danabé-Nieto Cros, Xavier Perron, Annabelle Mathieu-Denoncourt, Marylise Duperthuy Biofilm formation is a common strategy used bacteria in order to survive and persist in the environment. In Vibrio cholerae (V. cholerae), a Gram-negative pathogen responsible for the cholera disease, biofilm-like aggregates are important for the pathogenesis and disease transmission....
Wed, 08/14/2019 - 11:00
Vibrio parahaemolyticus is a leading cause of seafood-borne gastroenteritis. Given its natural presence in brackish waters, there is a need to develop operational forecast models that can sufficiently predict the bacterium’s spatial and temporal variation. This work attempted to develop V. parahaemolyticus prediction models using frequently measured time-indexed and -lagged water quality...
Tue, 08/13/2019 - 17:00
Adrián E. Velázquez-Lizárraga, José Luis Juárez-Morales, Ilie S. Racotta, Humberto Villarreal-Colmenares, Oswaldo Valdes-Lopez, Antonio Luna-González, Carmen Rodríguez-Jaramillo, Norma Estrada, Felipe Ascencio Acute hepatopancreatic necrosis disease (AHPND), caused marine bacteria Vibrio Parahaemolyticus, is a huge problem in shrimp farms. The V. parahaemolyticus infecting material is contained...
Mon, 08/12/2019 - 20:00
Vibrio parahaemolyticus (V. parahaemolyticus) is a leading cause of food poisoning and is of great importance to public health due to the frequency and seriousness of the diseases. The simple, timely and efficien...
Mon, 08/12/2019 - 17:00
Alyson R. Warr, Troy P. Hubbard, Diana Munera, Carlos J. Blondel, Pia Abel zur Wiesch, Sören Abel, Xiaoxue Wang, Brigid M. Davis, Matthew K. Waldor Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is an important food-borne pathogen that colonizes the colon. Transposon-insertion sequencing (TIS) was used to identify genes required for EHEC and E. coli K-12 growth in vitro and for EHEC growth in...
Mon, 08/12/2019 - 12:29
Cyclic di-GMP (c-di-GMP) is a bacterial second messenger molecule that is important in the biology of Vibrio cholerae, but the molecular mechanisms by which this molecule regulates downstream phenotypes have not been fully characterized. We have previously shown that the Vc2 c-di-GMP-binding riboswitch, encoded upstream of the gene tfoY, functions as an off-switch in response to c-di-GMP. However...
Fri, 08/09/2019 - 01:05
Available online 7 August 2019 Author(s): Tao Lei, Jumei Zhang, Fufeng Jiang, Min He, Haiyan Zeng, Moutong Chen, Shi Wu, Juan Wang, Yu Ding, Qingping WuAbstractThe plasmid-mediated colistin resistance gene mcr-1 has been identified in various Enterobacteriaceae species, which poses a great challenge to the public health. The present study aimed to investigate the prevalence of mcr-1 in Vibrio...
Wed, 08/07/2019 - 05:22
Abstract Aims The control of Listeria monocytogenes biofilm formation using lactocin AL705 bacteriocin at sub‐minimum inhibitory concentrations (MICs) through an antiquorum sensing strategy, was preliminarily investigated. Methods and Results The screening for biofilm formation of different Listeria species at 10°C allowed selecting L. monocytogenes FBUNT for its use as biofilm producer. MIC...
Fri, 08/02/2019 - 01:03
Available online 31 July 2019 Author(s): Shenyuan He, Xueyi Li, Cuiqin Li, Hong Deng, Yuyu Shao, Li YuanAbstractBenzo[a]pyrene (BaP, most toxic polycyclic aromatic hydrocarbon) is a global food-borne pollutant, and is associated with many diseases and gut microbiota disorders. The present study was designed to investigate the protective effects of isoorientin (ISO), a flavonoid compound in the...
Tue, 07/30/2019 - 17:00
Abraham Guerrero, Alexei Fedorovish Licea-Navarro, Ricardo González-Sánchez, Marcial Leonardo Lizárraga-Partida Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole...
Mon, 07/29/2019 - 11:30
Vibrio parahaemolyticus RIMD2210633 secretes both chitinase and chitin oligosaccharide deacetylase and produces β-N-acetyl-d-glucosaminyl-(1,4)-d-glucosamine (GlcNAc-GlcN) from chitin. Previously, we reported that GlcNAc-GlcN induces chitinase production by several strains of Vibrio harboring chitin oligosaccharide deacetylase genes (Hirano et al. Glycobiology 19:1049-1053, 2009). The...
Mon, 07/29/2019 - 07:51
Summary The opportunistic human pathogen Vibrio vulnificus inhabits warm coastal waters and asymptomatically colonizes seafood, most commonly oysters. We previously characterized an isolate that exhibited greater biofilm formation, aggregation and oyster colonization than its parent. This was due, in part, to the production of a Type IV Tad pilus (Iam). However, the locus lacked key processing...
Tue, 07/23/2019 - 13:05
Masmin preparation is a traditional method that has been practiced for many years to preserve skipjack tuna (Katsuwonus pelamis) mainly in Lakshadweep. However, the method is not evaluated scientifically for its quality concerning nutrition and environmental contamination. Thus, in the present study tuna fillets from four stages of Masmin preparation, namely fresh, boiling, smoking, and sun‐...
Mon, 07/22/2019 - 11:21
Outer membrane vesicles (OMVs) are continuously produced by Gram-negative bacteria, and are increasingly recognized as ubiquitous mediators of bacterial physiology. In particular, OMVs are powerful effectors in inter-organismal interactions, driven largely by their molecular contents. These impacts have been studied extensively in bacterial pathogenesis, but have not been well documented within...

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