Yersinia

Fri, 09/25/2020 - 16:00
The use of molecular diagnostics for pathogen detection in epidemiological studies have allowed us to get a wider view of the pathogens associated with diarrhea, but the presence of enteropathogens in asymptomatic individuals has raised several challenges in understanding the etiology of diarrhea, and the use of these platforms in clinical diagnosis as well. To characterize the presence of the...
Thu, 09/24/2020 - 08:05
by Vanessa Knittel, Pooja Sadana, Stephanie Seekircher, Anne-Sophie Stolle, Britta Körner, Marcel Volk, Cy M. Jeffries, Dmitri I. Svergun, Ann Kathrin Heroven, Andrea Scrima, Petra Dersch Type VI secretion systems (T6SSs) are complex macromolecular injection machines which are widespread in Gram-negative bacteria. They are involved in host-cell interactions and pathogenesis, required to eliminate...
Wed, 09/23/2020 - 10:03
The bacteriological diagnosis of intestinal bacterial infections has historically been based on culture on agar plates. However, culture may lack sensitivity, and some enteropathogens, such as pathovars of Escherichia coli, may escape routine diagnosis. Our goal was to evaluate the analytical performance of the Novodiag Bacterial GE+ kit for the detection of enteropathogenic bacteria in acute...
Wed, 09/09/2020 - 16:00
It has been previously shown that RskA, the anti-Sigma factor K of Mycobacterium tuberculosis, inhibits SigK and that mutations in RskA promote high expression of the SigK regulon. The latter observation led us to hypothesize that RskA mutations lead to loss of the anti-Sigma factor function. In this report, we used natural and artificial mutations in RskA to determine the basis of the SigK-RskA...
Tue, 08/25/2020 - 10:05
ABSTRACT Severe systemic bacterial infections result in colonization of deep tissues, which can be very difficult to eliminate with antibiotics. It remains unclear if this is because antibiotics are not reaching inhibitory concentrations within tissues, if subsets of bacteria are less susceptible to antibiotics, or if both contribute to limited treatment efficacy. To detect exposure to...
Wed, 08/19/2020 - 08:02
by Lovasoa N. Randriantseheno, Anjanirina Rahantamalala, Ando L. Randrianierenana, Minoarisoa Rajerison, Voahangy Andrianaivoarimanana Background Several tests are available for plague confirmation but bacteriological culture with Yersinia pestis strain isolation remains the gold standard according to the World Health Organization. However, this is a time consuming procedure; requiring specific...
Tue, 08/11/2020 - 10:03
Cohesion of biofilms made by Yersinia pestis and Yersinia pseudotuberculosis (Yptb) has been attributed solely to an extracellular polysaccharide matrix encoded by the hms genes (Hms-ECM). However, mutations in the Yptb BarA/UvrY/CsrB regulatory cascade enhance biofilm stability without dramatically increasing Hms-ECM production. We found that treatment with proteinase K enzyme effectively...
Sun, 07/19/2020 - 06:11
Author(s): Beverley C. Millar, John E. Moore Minimum inhibitory concentration to delafloxacin and ciprofloxacin were performed with Y. enterocolitica, Y. frederiksenii and Y. kristensenii. All organisms were sensitive to delafloxacin and ciprofloxacin. Our study indicates that delafloxacin may have a reasonable in vitro susceptibility profile against Yersinia among the species studied, which has...
Thu, 07/16/2020 - 00:00
Itaconic acid is an immunoregulatory metabolite produced by macrophages in response to pathogen invasion. It also exhibits antibacterial activity because it is an uncompetitive inhibitor of isocitrate lyase, whose activity is required for the glyoxylate shunt to be operational. Some bacteria, such as Yersinia pestis, encode enzymes that can degrade itaconic acid and therefore eliminate this...
Tue, 07/14/2020 - 06:14
Author(s): Suvi Joutsen, Per Johansson, Riikka Laukkanen-Ninios, Johanna Björkroth, Maria Fredriksson-Ahomaa Yersinia enterocolitica is the most common Yersinia species causing foodborne infections in humans. Pathogenic strains carry the chromosomal ail gene, which is essential for bacterial attachment to and invasion into host cells and for serum resistance. This gene is commonly amplified in...
Mon, 07/06/2020 - 06:12
Author(s): M.F. Peruzy, M. Aponte, Y.T.R. Proroga, F. Capuano, D. Cristiano, E. Delibato, K. Houf, N. Murru Conventional methods for Yersinia enterocolitica detection in food samples are generally considered inadequate. Problems arise from the presence of the so-called “background flora”, coupled to the low contamination level of the pathogen. Since, data on the microbial ecology occurring in...
Tue, 06/30/2020 - 16:00
CexE is a 12 kDa protein that was originally reported to be present in just three strains of enterotoxigenic Escherichia coli (ETEC); a frequent cause of diarrheal illnesses worldwide. However, an examination of sequenced genomes has revealed that CexE is actually present in a majority of ETEC strains. In addition, homologs of CexE are present in enteroaggregative E. coli (EAEC), Yersinia...
Fri, 06/19/2020 - 16:00
We report here the complete genome sequence and characterization of Yersinia bacteriophage vB_YenP_ϕ80-18. ϕ80-18 was isolated in 1991 using a Y. enterocolitica serotype O:8 strain 8081 as a host from a sewage sample in Turku, Finland, and based on its morphological and genomic features is classified as a podovirus. The genome is 42 kb in size and has 325 bp direct terminal repeats characteristic...
Wed, 06/10/2020 - 02:03
l-asparaginase (E.C.3.5.1.1) is an important enzyme that has been purified and characterized for over decades to study and evaluate its anti-carcinogenic activity against different lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and Hodgkin’s lymphoma. The ability of the enzyme to convert l-asparagine into aspartic acid and ammonia is the reason behind its anti-cancerous...
Sun, 05/24/2020 - 03:37
The occurrence of multidrug-resistant pathogenic bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Acinetobacter baumannii (MDRAB), extended-spectrum β-lactamase (ESBL) Escherichia coli, and Pseudomonas aeruginosa, has become a serious problem in animals and public. The objective of this study was to identify and isolate lactic acid bacterial (LAB) strains...
Fri, 05/22/2020 - 03:37
Background Additional treatment options for pneumonic plague, the most severe form of infection by Yersinia pestis, are needed, as past US Food and Drug Administration (FDA) approvals were not based on clinical trials that meet today’s standards, and multiple drugs are sought to counter resistance or use in special populations. Due to the sporadic nature of outbreaks and the low number of...
Fri, 05/22/2020 - 03:37
Background Ciprofloxacin and levofloxacin, 2 fluoroquinolone antimicrobials, are ≥90% effective for the treatment of inhalational plague when administered within 2–6 hours of fever onset in African green monkeys (AGM). Based on data in the AGM model, these antimicrobials were approved under the Food and Drug Administration’s Animal Efficacy Rule. However, that data did not address the issue of...
Tue, 05/12/2020 - 03:45
by Lamyaa Shaban, Giang T. Nguyen, Benjamin D. Mecsas-Faxon, Kenneth D. Swanson, Shumin Tan, Joan Mecsas Yersinia suppress neutrophil responses by using a type 3 secretion system (T3SS) to inject 6–7 Yersinia effector proteins (Yops) effectors into their cytoplasm. YopH is a tyrosine phosphatase that causes dephosphorylation of the adaptor protein SKAP2, among other targets in neutrophils. SKAP2...
Thu, 04/30/2020 - 03:43
Some biological activities of dihydroquercetin (DHQ), including antioxidative, antibacterial and anticancer activities, and the oxidative stability of butter oil supplemented with DHQ were evaluated. The DPPH* radical‐scavenging activity was high for DHQ, with an IC50 of 63.83 ± 3.11 μg/ml, compared to that of Trolox, which had an IC50 of 117.02 ± 5.19 μg/ml, over the concentrations studied (10–...
Thu, 04/30/2020 - 03:37
Rodents are a large group of mammals that can be carriers of zoonotic pathogens such as Yersinia strains that cause yersiniosis. The prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis was determined in 214 small wild rodents from south-eastern Poland. Samples were analyzed by precultivation and PCR. Nine (4.2%) Y. enterocolitica and one (0.5%) Y. pseudotuberculosis isolates...
Fri, 04/24/2020 - 03:34
Yersinia pestis, Brucella spp., and Bacillus anthracis are pathogens that can cause infectious zoonotic diseases with high mortality rates. An upconverting phosphor-based quantitative immunochromatographic (UPT-LF) assay, a point-of-care testing method suitable for resource-limited areas, was calibrated to quantitatively detect pathogenic bacteria. The bacterial purity or activity were ensured...
Wed, 04/22/2020 - 03:33
Brucellosis is a serious zoonosis occurring mainly in developing countries, and its diagnosis is largely dependent on serologic detection and bacterial culture. In this study, we developed the murine monoclonal antibodies (mAbs) against a conserved and major outer membrane protein 25 (Omp25) of Brucella species (B. spp.) for use in clinical diagnosis. The mAbs to Omp25 were produced by hybridoma...
Fri, 04/17/2020 - 03:26
HpaR, a MarR family transcriptional regulator, was first identified in Escherichia coli W for its regulation of the hpa-meta operon. Little else is known regarding its functionality. Here, we report that in Yersinia pseudotuberculosis, HpaR negatively regulates the hpa-meta operon similar to in E. coli W. To investigate additional functions of HpaR, RNA sequencing was performed for both the wild-...
Tue, 04/14/2020 - 03:29
Aims of the study The ability of Yersinia enterocolitica strains to form biofilms and the capacity of different alkaloids to inhibit biofilm formation were investigated. Methods and Results The capacity to form biofilm on polystyrene of 31 Y. enterocolitica strains was evaluated. Biofilm and quorum sensing (QS) inhibition of 17 alkaloids were assayed; furthermore, minimum biofilm inhibitory...
Wed, 04/08/2020 - 03:33
Yersinia enterocolitica exhibits a dual lifestyle, existing as both a saprophyte and a pathogen colonizing different niches within a host organism. OmpR has been recognized as a regulator that controls the expression of genes involved in many different cellular processes and the virulence of pathogenic bacteria. Here, we have examined the influence of OmpR and varying temperature (26°C vs. 37°C)...
Wed, 04/08/2020 - 03:32
This study evaluated the efficacy of lactic acid bacteria (LAB) isolated from traditional fermented foods and beverage of Ladakh as biocontrol agents against Yersinia enterocolitica. The antagonistic activities of 46 LAB isolates were screened for their ability to inhibit the growth of Y. enterocolitica at refrigeration temperatures using agar spot tests. On the basis of the 16S rDNA sequences,...
Wed, 04/08/2020 - 03:32
This study evaluated the efficacy of lactic acid bacteria (LAB) isolated from traditional fermented foods and beverage of Ladakh as biocontrol agents against Yersinia enterocolitica. The antagonistic activities of 46 LAB isolates were screened for their ability to inhibit the growth of Y. enterocolitica at refrigeration temperatures using agar spot tests. On the basis of the 16S rDNA sequences,...
Tue, 03/24/2020 - 04:03
While alternating between insects and mammals during its lifecycle, Yersinia pestis, the flea transmitted bacterium that causes plague, regulates its gene expression appropriately to adapt to these two physiologically disparate host environments. In fleas competent to transmit Y. pestis, low GC content genes y3555, y3551 and y3550 are highly transcribed, suggesting that these genes have a highly...
Tue, 03/24/2020 - 04:03
Oral administration is a preferred model for studying infection by bacterial enteropathogens such as Yersinia spp. In the mouse model, the most frequent method for oral infection consists of oral gavage with a feeding needle directly introduced in the animal stomach via the esophagus. In this study, we compared needle gavage to bread feeding as an alternative mode of bacterial administration....
Fri, 03/06/2020 - 03:46
Yersinia enterocolitica is generally considered an important food-borne pathogen worldwide, especially in the European Union. A lytic Yersinia phage X1 (Viruses; dsDNA viruses, no RNA stage; Caudovirales; and Myoviridae) was isolated. Phage X1 showed a broad host range and could effectively lyse 27/51 Y. enterocolitica strains covering various serotypes that cause yersiniosis in humans and...
Wed, 02/05/2020 - 03:26
Fast response to environmental changes plays a key role in the transmission and pathogenesis of Yersinia enterocolitica. Osmoregulated periplasmic glucans (OPGs) are known to be involved in environmental perception of several Enterobacteriaceae pathogens; however, the biological function of OPGs in Y. enterocolitica is still unclear. In this study, we investigated the role of OPGs in Y....
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:25
Poultry meat is one of the most important sources of infection of Yersinia spp. for humans. The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay. Also, biochemical methods were used for biotyping. A total of 100 chicken thigh meat samples were collected randomly from retail...
Sun, 01/26/2020 - 03:33
Author(s): Elisabetta Razzuoli, Walter Vencia, Paola Modesto, Giulia Franzoni, Silvia Dei Giudici, Erica Parisi, Angelo Ferrari, Massimo AmadoriAbstractGut is often subject to infection by different pathogens like Y. enterocolitica. To date, biotypes (BTs) 1A have been considered as non-pathogenic, because they do not express plasmid of virulence pYV; however, BTs 1A strains present other...
Fri, 01/24/2020 - 03:24
Yersinia enterocolitica, a zoonotic foodborne pathogen, is able to withstand low temperatures. This psychrotrophic ability allows it to multiply in food stored in refrigerators. However, little is known about the Y. enterocolitica cold response. In this study, isolate-specific behavior at 4°C was demonstrated and the cold response was investigated by examining changes in phenotype, gene...
Sat, 01/18/2020 - 03:33
by Christian Twittenhoff, Ann Kathrin Heroven, Sabrina Mühlen, Petra Dersch, Franz Narberhaus Frequent transitions of bacterial pathogens between their warm-blooded host and external reservoirs are accompanied by abrupt temperature shifts. A temperature of 37°C serves as reliable signal for ingestion by a mammalian host, which induces a major reprogramming of bacterial gene expression and...
Tue, 12/24/2019 - 03:35
by Diana Hooker-Romero, Erin Mettert, Leah Schwiesow, David Balderas, Pablo A. Alvarez, Anadin Kicin, Azuah L. Gonzalez, Gregory V. Plano, Patricia J. Kiley, Victoria Auerbuch The enteropathogen Yersinia pseudotuberculosis and the related plague agent Y. pestis require the Ysc type III secretion system (T3SS) to subvert phagocyte defense mechanisms and cause disease. Yet type III secretion (T3S)...
Sat, 12/21/2019 - 04:08
by Zoltán Attila Nagy, Dávid Szakács, Eszter Boros, Dávid Héja, Eszter Vígh, Noémi Sándor, Mihály Józsi, Gábor Oroszlán, József Dobó, Péter Gál, Gábor Pál Ecotin is a serine protease inhibitor produced by hundreds of microbial species, including pathogens. Here we show, that ecotin orthologs from Escherichia coli, Yersinia pestis, Pseudomonas aeruginosa and Leishmania major are potent inhibitors...
Wed, 11/13/2019 - 03:35
Author(s): Erwin M. Berendsen, Evgeni Levin, René Braakman, Andrei Prodan, Hans C. van Leeuwen, Armand PaauwAbstractTo improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass...
Wed, 11/06/2019 - 03:27
ABSTRACT A thorough understanding of the mechanisms of Rcs and EnvZ/OmpR phosphorelay systems that allow Yersinia enterocolitica to thrive in various environments is crucial to prevent and control Y. enterocolitica infections. In this study, we showed that RcsB and OmpR have the ability to function differently in modulating a diverse array of physiological processes in Y. enterocolitica. The...
Thu, 10/17/2019 - 03:31
The sugar glider (Petaurus breviceps) is a small, arboreal, nocturnal, gliding mammalian possum belonging to the marsupial infraclass. Exotic marsupials, including sugar gliders, are becoming popular companion pets and, consequently, the risk of potential infections that can be transmitted to humans should be investigated. Data on the role of the sugar glider as a possible carrier of pathogenic...
Tue, 10/08/2019 - 03:38
Author(s): Cecilia Lucero Estrada, Gabriela Isabel Favier, María Esther EscuderoAbstractThis study is aimed at offering an overview of the prevalence of Yersinia enterocolitica and related species in San Luis, Argentina, from samples of diverse origin received in our laboratory between 1984 and 2014, and providing an analysis of the distribution of Yersinia isolates according to their isolation...
Sat, 09/28/2019 - 03:23
Aims This study investigated the efficacy of Hydrogen Peroxide Vapour (HPV) at inactivating Hazard Group 3 bacteria that have been presented dried from their growth medium to present a realistic challenge. Methods and Results HPV technology (Bioquell) was used to decontaminate a class III microbiological safety cabinet containing biological indicators (BIs) made by drying standard working...
Fri, 09/20/2019 - 03:43
To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also...
Fri, 09/20/2019 - 03:43
In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (10069) engineered with yopK yopJ asd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae. 10069 bacteria harboring the pYA5199 plasmid constitutively synthesized the...
Thu, 09/19/2019 - 11:00
In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (10069) engineered with yopK yopJ asd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae. 10069 bacteria harboring the pYA5199 plasmid constitutively synthesized the...
Thu, 09/19/2019 - 11:00
To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also...
Wed, 09/18/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 10, Page 1683-1687, October 2019. The effect of the virulence plasmid pYV and calcium ions on biofilm of Yersinia enterocolitica biofilm formation was determined using a microtiter plate assay. Loss of the pYV plasmid prevented biofilm formation and the presence of Ca2+ enhanced biofilm formation in cultures containing the pYV plasmid. Scanning...
Sat, 09/14/2019 - 03:36
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Sat, 09/14/2019 - 03:34
Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz BanaeiAbstractThough the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for...

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