Yersinia

Wed, 02/05/2020 - 03:26
Fast response to environmental changes plays a key role in the transmission and pathogenesis of Yersinia enterocolitica. Osmoregulated periplasmic glucans (OPGs) are known to be involved in environmental perception of several Enterobacteriaceae pathogens; however, the biological function of OPGs in Y. enterocolitica is still unclear. In this study, we investigated the role of OPGs in Y....
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:25
Poultry meat is one of the most important sources of infection of Yersinia spp. for humans. The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay. Also, biochemical methods were used for biotyping. A total of 100 chicken thigh meat samples were collected randomly from retail...
Sun, 01/26/2020 - 03:33
Author(s): Elisabetta Razzuoli, Walter Vencia, Paola Modesto, Giulia Franzoni, Silvia Dei Giudici, Erica Parisi, Angelo Ferrari, Massimo AmadoriAbstractGut is often subject to infection by different pathogens like Y. enterocolitica. To date, biotypes (BTs) 1A have been considered as non-pathogenic, because they do not express plasmid of virulence pYV; however, BTs 1A strains present other...
Fri, 01/24/2020 - 03:24
Yersinia enterocolitica, a zoonotic foodborne pathogen, is able to withstand low temperatures. This psychrotrophic ability allows it to multiply in food stored in refrigerators. However, little is known about the Y. enterocolitica cold response. In this study, isolate-specific behavior at 4°C was demonstrated and the cold response was investigated by examining changes in phenotype, gene...
Sat, 01/18/2020 - 03:33
by Christian Twittenhoff, Ann Kathrin Heroven, Sabrina Mühlen, Petra Dersch, Franz Narberhaus Frequent transitions of bacterial pathogens between their warm-blooded host and external reservoirs are accompanied by abrupt temperature shifts. A temperature of 37°C serves as reliable signal for ingestion by a mammalian host, which induces a major reprogramming of bacterial gene expression and...
Tue, 12/24/2019 - 03:35
by Diana Hooker-Romero, Erin Mettert, Leah Schwiesow, David Balderas, Pablo A. Alvarez, Anadin Kicin, Azuah L. Gonzalez, Gregory V. Plano, Patricia J. Kiley, Victoria Auerbuch The enteropathogen Yersinia pseudotuberculosis and the related plague agent Y. pestis require the Ysc type III secretion system (T3SS) to subvert phagocyte defense mechanisms and cause disease. Yet type III secretion (T3S)...
Sat, 12/21/2019 - 04:08
by Zoltán Attila Nagy, Dávid Szakács, Eszter Boros, Dávid Héja, Eszter Vígh, Noémi Sándor, Mihály Józsi, Gábor Oroszlán, József Dobó, Péter Gál, Gábor Pál Ecotin is a serine protease inhibitor produced by hundreds of microbial species, including pathogens. Here we show, that ecotin orthologs from Escherichia coli, Yersinia pestis, Pseudomonas aeruginosa and Leishmania major are potent inhibitors...
Wed, 11/13/2019 - 03:35
Author(s): Erwin M. Berendsen, Evgeni Levin, René Braakman, Andrei Prodan, Hans C. van Leeuwen, Armand PaauwAbstractTo improve the preparedness against exposure to highly pathogenic bacteria and to anticipate the wide variety of bacteria that can cause bloodstream infections (BSIs), a safe, unbiased and highly accurate identification method was developed. Our liquid chromatography-tandem mass...
Wed, 11/06/2019 - 03:27
ABSTRACT A thorough understanding of the mechanisms of Rcs and EnvZ/OmpR phosphorelay systems that allow Yersinia enterocolitica to thrive in various environments is crucial to prevent and control Y. enterocolitica infections. In this study, we showed that RcsB and OmpR have the ability to function differently in modulating a diverse array of physiological processes in Y. enterocolitica. The...
Thu, 10/17/2019 - 03:31
The sugar glider (Petaurus breviceps) is a small, arboreal, nocturnal, gliding mammalian possum belonging to the marsupial infraclass. Exotic marsupials, including sugar gliders, are becoming popular companion pets and, consequently, the risk of potential infections that can be transmitted to humans should be investigated. Data on the role of the sugar glider as a possible carrier of pathogenic...
Tue, 10/08/2019 - 03:38
Author(s): Cecilia Lucero Estrada, Gabriela Isabel Favier, María Esther EscuderoAbstractThis study is aimed at offering an overview of the prevalence of Yersinia enterocolitica and related species in San Luis, Argentina, from samples of diverse origin received in our laboratory between 1984 and 2014, and providing an analysis of the distribution of Yersinia isolates according to their isolation...
Sat, 09/28/2019 - 03:23
Aims This study investigated the efficacy of Hydrogen Peroxide Vapour (HPV) at inactivating Hazard Group 3 bacteria that have been presented dried from their growth medium to present a realistic challenge. Methods and Results HPV technology (Bioquell) was used to decontaminate a class III microbiological safety cabinet containing biological indicators (BIs) made by drying standard working...
Fri, 09/20/2019 - 03:43
To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also...
Fri, 09/20/2019 - 03:43
In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (10069) engineered with yopK yopJ asd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae. 10069 bacteria harboring the pYA5199 plasmid constitutively synthesized the...
Thu, 09/19/2019 - 11:00
In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (10069) engineered with yopK yopJ asd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae. 10069 bacteria harboring the pYA5199 plasmid constitutively synthesized the...
Thu, 09/19/2019 - 11:00
To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also...
Wed, 09/18/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 10, Page 1683-1687, October 2019. The effect of the virulence plasmid pYV and calcium ions on biofilm of Yersinia enterocolitica biofilm formation was determined using a microtiter plate assay. Loss of the pYV plasmid prevented biofilm formation and the presence of Ca2+ enhanced biofilm formation in cultures containing the pYV plasmid. Scanning...
Sat, 09/14/2019 - 03:36
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Sat, 09/14/2019 - 03:34
Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz BanaeiAbstractThough the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for...
Thu, 09/12/2019 - 20:00
Many pathogenic bacteria use the type III secretion system (T3SS) injectisome to manipulate host cells by injecting virulence-promoting effector proteins into the host cytosol. The T3SS is activated upon host cell contact, and its activation is accompanied by an arrest of cell division; hence, many species maintain a T3SS-inactive sibling population to propagate efficiently within the host. The...
Sat, 08/10/2019 - 01:05
Available online 7 August 2019 Author(s): Julia Reichel, Corinna Kehrenberg, Carsten KrischekAbstractUltraviolet (UV) irradiation has gained interest as a decontamination method for food for several years. This study investigated how UV-C affected the microbial load of pork, inoculated with Yersinia (Y.) enterocolitica and Brochothrix (B.) thermosphacta. The initial effect as well as the effect...
Mon, 07/29/2019 - 03:00
, Ahead of Print. Pigs are considered the main reservoir of Yersinia enterocolitica, and hence, understanding the ecology of this foodborne pathogen at the farm level is crucial. We calculated Bayesian estimates for the ability of a commercial enzyme-linked immunosorbent assay (ELISA) diagnostic test kit to detect antibodies against pathogenic Yersinia in pigs. The sensitivity and specificity of...
Tue, 07/09/2019 - 17:00
Xiaojie Jiang, Riikka Keto-Timonen, Mikael Skurnik, Hannu Korkeala Yersinia pseudotuberculosis is an important foodborne pathogen threatening modern food safety due to its ability to survive and grow at low temperatures. DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total of five DEAD-box RNA helicase...
Fri, 06/07/2019 - 17:00
Maria Kusmierek, Jörn Hoßmann, Rebekka Witte, Wiebke Opitz, Ines Vollmer, Marcel Volk, Ann Kathrin Heroven, Hans Wolf-Watz, Petra Dersch Numerous Gram-negative pathogens use a Type III Secretion System (T3SS) to promote virulence injecting effector proteins into targeted host cells, which subvert host cell processes. Expression of T3SS and the effectors is triggered upon host cell contact, but...
Thu, 06/06/2019 - 01:11
Available online 4 June 2019 Author(s): Erwin Bohn, Michael Sonnabend, Kristina Klein, Ingo B. AutenriethAbstractType III secretion systems (T3SS) play a crucial role for virulence in many Gram-negative bacteria. After tight bacterial contact to host cells, the T3SS injects effector proteins into the host cells, which leads to cell invasion, tissue destruction and/or immune evasion. Over the last...
Mon, 06/03/2019 - 01:12
Available online 1 June 2019 Author(s): Karolin Leibiger, Jonas Malte Schweers, Monika SchützAbstractBacteria often express numerous virulence factors. These virulence factors make them successful pathogens, by e.g. mediating attachment to host cells and thereby facilitating persistence or invasion, or by contributing to the evasion of the host immune system to allow proliferation and spread...
Tue, 05/28/2019 - 20:00
Yersinia enterocolitica is a major agent of foodborne diseases worldwide. Prophage plays an important role in the genetic evolution of the bacterial genome. Little is known about the genetic information about prophages in the genome of Y. enterocolitica, and no pathogenic Y. enterocolitica prophages have been described. In this study, we induced and described the genomes of six prophages from...
Wed, 05/22/2019 - 17:00
Katharina Loreck, Sylvia Mitrenga, Diana Meemken, Regina Heinze, Annett Reissig, Elke Mueller, Ralf Ehricht, Claudia Engemann, Matthias Greiner In order to monitor the occurrence of zoonotic agents in pig herds as well as to improve herd health management, the development of new cost-effective diagnostic methods for pigs is necessary. In this study, a protein microarray-based assay for the...
Thu, 04/25/2019 - 20:00
Role of Starter Cultures on the Safety of Fermented Meat Products Marta Laranjo1*, Maria Eduarda Potes1,2 and Miguel Elias1,3 1ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Pólo da Mitra, Évora, Portugal 2Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Mon, 04/08/2019 - 20:00
Gamma Irradiation Influences the Survival and Regrowth of Antibiotic-Resistant Bacteria and Antibiotic-Resistance Genes on Romaine Lettuce Vaishali Dharmarha1, Giselle Guron2, Renee R. Boyer1, Brendan A. Niemira3, Amy Pruden2, Laura K. Strawn1 and Monica A. Ponder1* 1Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, United States 2Department of Civil and Environmental...
Mon, 03/18/2019 - 01:14
Publication date: Available online 16 March 2019Source: Author(s): Heba A. Ahmed, Asmaa B.M.B. Tahoun, Rasha M.M. Abou Elez, Marwa I. Abd El-Hamid, Samah S. Abd EllatifAbstractYersinia enterocolitica was isolated from raw milk and dairy products from 10% of examined samples. The highest isolation rate was 22%, from raw milk, followed by 12%, 4% and 2% from fermented milk (Rayeb), pasteurised milk...
Sun, 03/17/2019 - 01:13
LWT
Publication date: Available online 15 March 2019Source: LWTAuthor(s): Abdus Sobhan, Jinyoung Lee, Mi-Kyung Park, Jun-Hyun OhAbstractThe goal of this research was to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor method and to employ it to a Kimchi product for Yersinia enterocolitica detection. A SWCNT-based biosensor was fabricated with SWCNTs immobilized with anti-Yersinia...
Fri, 03/15/2019 - 17:00
Antoine Dujardin, Peter De Wolf, Frank Lafont, Vincent Dupres In the last 20 years, atomic force microscopy (AFM) has emerged as a ubiquitous technique in biological research, allowing the analysis of biological samples under near-physiological conditions from single molecules to living cells. Despite its growing use, the low process throughput remains a major drawback. Here, we propose a...
Mon, 03/11/2019 - 11:32
The phage shock protein (Psp) system is a stress response pathway that senses and responds to inner membrane damage. The genetic components of the Psp system are present in several clinically relevant Gram-negative bacteria, including Vibrio cholerae. However, most of the current knowledge about the Psp response stems from in vitro studies in Escherichia coli and Yersinia enterocolitica. In fact...
Sun, 03/10/2019 - 01:13
Available online 9 March 2019 Author(s): Reinhard Sting, Tobias Eisenberg, Maja HrubenjaAbstractAnalyses of short subunit gene sequences have been established for taxonomic classification and identification of bacteria and fungi. To produce partial bacterial ribosomal 16S rRNA and rpoB and fungal ribosomal ITS/LSU gene sequences for DNA sequencing, real-time PCR assays supplemented with the...
Fri, 03/08/2019 - 07:54
Significance and Impact of the Study: Alkaloids are abundant secondary metabolites in plants and represent one of the most widespread class of compounds endowed with multiple and varied pharmacological properties. In this work, we propose two aporphinoid alkaloids extracted from plants as new antimicrobial agents. Oliveridine and pachypodanthine inhibited Yersinia enterocolitica growth for up to...
Fri, 03/08/2019 - 01:14
The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in Yersinia pseudotuberculosis induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content...
Sun, 02/24/2019 - 01:12
Available online 21 February 2019 Author(s): Santosh Keisam, Ngangyola Tuikhar, Giasuddin Ahmed, Kumaraswamy JeyaramAbstractThe microbial risk involved with natural food fermentation is largely unknown. Here, we report the prevalence of enteric bacterial pathogens in the traditional fermented foods marketed in Northeast region of India. A total of 682 samples of 39 food types (broadly categorized...
Wed, 02/06/2019 - 01:08
Available online 4 February 2019 Author(s): Anne Mette Jakobsen, Martin Iain Bahl, Tasja Buschhardt, Tina Beck Hansen, Waleed Abu Al-Soud, Asker D. Brejnrod, Søren J. Sørensen, Truls Nesbakken, Søren AaboAbstractTonsils in the oral cavity are an important source of contamination during pig slaughter, but have not received as much attention as faecal contamination. In the present study, ten pigs...
Fri, 02/01/2019 - 01:14
LWT
Publication date: Available online 30 January 2019Source: LWTAuthor(s): Kalaiselvi Ignasimuthu, Rajalakshmi Prakash, Pushpa S. Murthy, Nagarajan SubbanAbstractThe polyphenol acetates derived from green tea (Camellia sinensis. L) catechins, effectively increases the bioaccessibility as evaluated by simulated invitro enzymatic digestion method. The acetylated polyphenols showed significantly higher...
Fri, 02/01/2019 - 01:12
Publication date: February 2019 , Volume 116Author(s): Surya Chandra Rao Thumu, Prakash M. HalamiAbstractThree strains of Lactobacillus comprising Lactobacillus salivarius (CHS-1E and CH7-1E) and Lactobacillus reuteri (CH2-2) previously isolated from chicken meat were analyzed for their transferability of antibiotic resistance (AR) genes to pathogenic strains under in vivo, in vitro, and during...
Wed, 12/19/2018 - 11:00
Yersinia pseudotuberculosis is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer’s patches to...
Wed, 12/19/2018 - 01:10
Available online 17 December 2018 Author(s): Franziska Koch, Claudia Wiacek, Peggy G. BraunAbstractThe aim of the presented study was to investigate the impact of pulsed light on the reduction of Salmonella Typhimurium and Yersinia enterocolitica on pork skin and loin. Fluences of 0.52 to 19.11 J/cm2 were applied to the pathogen-inoculated products to perform microbiological studies, as well as...
Thu, 12/06/2018 - 01:10
Available online 16 November 2018 Author(s): Marc Schulte, Torsten Sterzenbach, Katarzyna Miskiewicz, Laura Elpers, Michael Hensel, Nicole HansmeierAbstractThe expression of bacterial virulence factors is controlled in response to host or environmental factors and most virulence genes are not expressed under laboratory conditions. Investigations of molecular structures and cellular functions of...
Wed, 12/05/2018 - 01:08
Available online 3 December 2018 Author(s): Triveni Kalleshamurthy, Chaithra Yaranna, Ranjitha Shekar, Krithiga Natesan, Swati Sahay, Bibek Ranjan Shome, Habibur Rahman, Sukhadeo B. Barbuddhe, Nagendra Nath Barman, Sailendra Kumar Das, Rajeswari ShomeAbstractBrucellosis in pigs, caused by the bacterium Brucella suis, is an important zoonotic infection. In the present study, fluorescence...
Wed, 12/05/2018 - 01:08
Publication date: April 2019 , Volume 78Author(s): Mikaela Sauvala, Sauli Laaksonen, Riikka Laukkanen-Ninios, Katri Jalava, Roger Stephan, Maria Fredriksson-AhomaaAbstractHunting is currently a very popular activity, and interest in game meat is increasing. However, only limited research is available on the bacterial quality and safety of moose (Alces alces) and white-tailed deer (Odocoileus...
Wed, 12/05/2018 - 01:08
Available online 12 January 2018 Author(s): Saija Hallanvuo, Mirkka Herranen, Anniina Jaakkonen, Maria Nummela, Jukka Ranta, Interlaboratory study group, Nadine Botteldoorn, Lieven De Zutter, Maria Fredriksson-Ahomaa, Stefan Hertwig, Gro S. Johannessen, Martina Ludewig, Ute Messelhäußer, Pia Sigvart-Mattila, Susanne Thisted-Lambertz, Tiina Thure, Elina VatunenAbstractEN ISO 10273 method for the...
Tue, 11/27/2018 - 01:12
Jussa-Pekka Virtanen, Riikka Keto-Timonen, Kaisa Jaakkola, Noora Salin, Hannu Korkeala Yersinia pseudotuberculosis is a bacterium that not only survives, but also thrives, proliferates, and remains infective at cold-storage temperatures, making it an adept foodborne pathogen. We analyzed the differences in gene expression between Y. pseudotuberculosis IP32953 grown at 3 and 28°C to investigate...

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