Research Publications (Food Safety)

Bacteriological methods for the identification of Yersinia enterocolitica are laborious and time-consuming. The aim of this study was to compare Y. enterocolitica DNA isolation from swabs without pre-enrichment on selective media with isolation preceded by warm and cold enrichment. The material for the study consisted of 150 rectal swabs taken from 50 clinically healthy fattening pigs. Forty-one Y.

Polymyxin is the “last resort” of antibiotics. The self-induced resistance to polymyxin in Gram-negative bacteria could be mediated by lipopolysaccharide (LPS) modification, which is regulated by the two-component system, PhoP/PhoQ. Yersinia enterocolitica is a common foodborne pathogen. However, PhoP/PhoQ has not been thoroughly studied in Y. enterocolitica. In this study, the functions of PhoP/PhoQ in Y. enterocolitica intrinsic resistance were investigated. The resistance of Y.

Yersinia (Y.) enterocolitica, an etiological agent of yersiniosis, is a bacterium whose pathogenicity is determined, among other things, by its ability to produce toxins. The aim of this article was to present the most important toxins that are produced by biotype 1A strains of Y. enterocolitica, and to discuss their role in the pathogenesis of yersiniosis. Y.

Yersinia adhesin A (YadA) is a key virulence factor of Yersinia enterocolitica and Yersinia pseudotuberculosis. YadA is a trimeric autotransporter adhesin, a class of adhesins that have been shown to enable many Gram-negative pathogens to adhere to/interact with the host extracellular matrix proteins such as collagen, vitronectin, and fibronectin.

Molecular Microbiology, Accepted Article. YscX was discovered as an essential part of the Yersinia type III secretion system about 20 years ago. It is required for substrate secretion and is exported itself. Despite this central role, its precise function and mode of action remains unknown. In order to address this knowledge gap, this present study refocused attention on YscX to build on the recent advances in the understanding of YscX function.

Zoonoses and Public Health, Volume 69, Issue 1, Page 61-70, February 2022.

Journal of Food Processing and Preservation, Accepted Article.

Journal of Applied Microbiology, Volume 132, Issue 1, Page 209-220, January 2022.

Aquaculture Nutrition, Volume 27, Issue 6, Page 2587-2599, December 2021.

Hemachromatosis (iron-overload) increases host susceptibility to siderophilic bacterial infections that cause serious complications, but the underlying mechanisms remain elusive. The present study demonstrates that oral infection with hyperyersiniabactin (Ybt) producing Yersinia pseudotuberculosis Δfur mutant (termed Δfur) results in severe systemic infection and acute mortality to hemochromatotic mice due to rapid...

Journal of Food Processing and Preservation, Accepted Article.

Yersinia pseudotuberculosis is an important animal pathogen, particularly for birds, rodents, and monkeys, which is also able to infect humans. Indeed, an increasing number of reports have been published on zoo animals that were killed by this species. One option to treat diseased animals is the application of strictly lytic (virulent) phages. However, thus far relatively few phages infecting Y. pseudotuberculosis have been isolated and characterized. To determine the prevalence of Y.

Hemachromatosis (iron-overload) increases host susceptibility to siderophilic bacterial infections that cause serious complications, but the underlying mechanisms remain elusive. The present study demonstrates that oral infection with hyperyersiniabactin (Ybt) producing Yersinia pseudotuberculosis Δfur mutant (termed Δfur) results in severe systemic infection and acute mortality to hemochromatotic mice due to rapid...

Author(s): Bruna Torres Furtado Martins, João Luiz de Meirelles, Wellington Pine Omori, Rafael R. de Oliveira, Ricardo Seiti Yamatogi, Douglas Ruben Call, Luís Augusto Nero

Author(s): Shenmin Kang, Xuejiao Li, Zeyu Xing, Xue Liu, Xiangyang Bai, Yanpeng Yang, Du Guo, Xiaodong Xia, Chunling Zhang, Chao Shi

by Timothy H. Chu, Camille Khairallah, Jason Shieh, Rhea Cho, Zhijuan Qiu, Yue Zhang, Onur Eskiocak, David G. Thanassi, Mark H. Kaplan, Semir Beyaz, Vincent W. Yang, James B. Bliska, Brian S. Sheridan

Many pathogens use M cells to access the underlying Peyer’s patches and spread to systemic sites via the lymph as demonstrated by ligated loop murine intestinal models. However, the study of interactions between M cells and microbial pathogens has stalled due to the lack of cell culture systems.

by Indra Bekere, Jiabin Huang, Marie Schnapp, Maren Rudolph, Laura Berneking, Klaus Ruckdeschel, Adam Grundhoff, Thomas Günther, Nicole Fischer, Martin Aepfelbacher

by Stephan Pienkoß, Soheila Javadi, Paweena Chaoprasid, Thomas Nolte, Christian Twittenhoff, Petra Dersch, Franz Narberhaus

by David M. Bland, Adélaïde Miarinjara, Christopher F. Bosio, Jeanette Calarco, B. Joseph Hinnebusch

Author(s): Mi Wu, Lu Tian, Jiapeng Fu, Sichen Liao, Hui Li, Zhongchao Gai, Guoli Gong

Cellular ionic concentrations are a central factor orchestrating host innate immunity, but no pathogenic mechanism that perturbs host innate immunity by directly targeting metal ions has yet been described. Here, we report a unique virulence strategy of Yersinia pseudotuberculosis (Yptb) involving modulation of the availability of Mn2+, an immunostimulatory metal...

Avian pathogenic E. coli (APEC) caused avian colibacillosis is mostly common in poultry industry worldwide. APEC virulence factors lead to pathogenesis and the quorum sensing (QS) system is actively involved in the regulation of these virulence factors. Signaling molecules in QS are known as autoinducers (AIs). In QS-1, E. coli encodes a single LuxR homolog, i.e., SdiA, but does not express the LuxI homolog, an acyl-homoserine lactone (AHL) synthase of producing AI-1.