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Bacterial pathogens

Thu, 05/16/2019 - 01:08
Publication date: Available online 13 May 2019Source: LWTAuthor(s): Yiming Feng, Luis Alberto Ibarra-Sánchez, Lily Luu, Michael J. Miller, Youngsoo LeeAbstractNisin-loaded zein microcapsules were prepared in a microfluidic chip using an internal phase separation method. The release profiles of nisin from the microcapsules were modified by varying nisin loading and flow rate during the...
Thu, 05/16/2019 - 01:08
Publication date: Available online 13 May 2019Source: LWTAuthor(s): Seyed Mohammad Bagher Hashemi, Reza Roohi, Mohammad Reza Mahmoudi, Daniel GranatoAbstractInactivation of Listeria monocytogenes (serotype 4 ab), Shigella sonnei, Byssochlamys fulva and Saccharomyces cerevisiae and the degradation of β-carotene and ascorbic acid in tangerine juice subjected to pulsed-thermosonication were modeled...
Thu, 05/16/2019 - 01:07
Available online 15 May 2019 Author(s): Narges Elahi, Mohammad Hadi Baghersad, Mehdi KamaliAbstractEarly detection of infectious bacteria is a necessity for combating infectious diseases. Due to low infectious dose of Shigella, rapid and sensitive detection is needed. Compared to the presented genes, Spa gene can be introduced as a novel sequence for all species of Shigella detection. Herein, the...
Thu, 05/16/2019 - 01:07
Available online 14 May 2019 Author(s): Sheila B. Buijs, Mirjam H.A. Hermans, Nabila Agni, Maaike C. de Vries, Andy I.M. Hoepelman, Jan Jelrik Oosterheert, Peter C. WeverAbstractIntroductionCoxiella burnetii, the causative pathogen of Q fever, is regularly detected in throat swabs from patients without serological evidence of Q fever infection. C. burnetii is also frequently found in bulk tank...
Thu, 05/16/2019 - 00:00
Sensitive detection of pathogenic bacteria is of great significance for food safety and public health. This study aimed to report a novel F0F1–ATPase aptasensor based on quantum dots (QDs) for quantitative detection of Vibrio parahaemolyticus. The fluorescent QDs were labeled onto the surface of chromatophores to act as an indicator. Aptamer target to V. parahaemolyticus was...
Thu, 05/16/2019 - 00:00
Abstract In the present study, gelatin–carboxymethyl cellulose blend film was synthesized, characterized and applied for the first time to preserve cherry tomatoes (Solanum lycopersicum var. cerasiforme) and grapes (Vitis vinifera). Gelatin (Gel) film forming solution was incorporated with carboxymethyl cellulose (CMC) at three volume per volume (Gel:CMC) ratios, namely 75:25,...
Wed, 05/15/2019 - 20:00
Bacterial pathogens maintain disulfide bonds for protein stability and functions that are required for pathogenesis. Vibrio parahaemolyticus is a Gram-negative pathogen that causes food-borne gastroenteritis and is also an important opportunistic pathogen of aquatic animals. Two genes encoding the disulfide bond formation protein A, DsbA, are predicted to be encoded in the V. parahaemolyticus...
Wed, 05/15/2019 - 01:14
Available online 13 May 2019 Author(s): Adriana Antunes-Rohling, Ángela Artaiz, Silvia Calero, Nabil Halaihel, Silvia Guillén, Javier Raso, Ignacio Álvarez, Guillermo CebriánAbstractMarket globalization and changes in purchasing habits pose a challenge to the fishery industry because of the short shelf life of fish products. In view of this scenario, it would be very helpful if tools capable of...
Wed, 05/15/2019 - 01:11
Available online 13 May 2019 Author(s): Yuqiao Jin, Juming TangAbstractSalmonella enterica is a major cause of foodborne pathogen contamination in the United States. The heat resistance of Salmonella increases as aw decreases, leading to a significant challenge in the design of thermal processes. The aluminum thermal death time (TDT) cell is a common experimental tool to study the isothermal...
Wed, 05/15/2019 - 00:00
The rapid and sensitive detection of foodborne pathogens is one of the most important issues in food safety control. In this work, we developed a novel fluorescence immunoassay method for the sensitive detection of Salmonella choleraesuis. The method uses the fluorescent signals of histone-ds-poly(AT)-templated copper nanoparticles (His-pAT CuNP) as signal transducers and glucose oxidase as an...
Wed, 05/15/2019 - 00:00
Following the persistent detection of Listeria monocytogenes in raw bovine milk sold through a vending machine, the 120 lactating cows of the herd producing the milk were subjected to bacteriological investigation. A single cow with subclinical mastitis (1.2–1.3 × 105 somatic cells/mL) and persistent L. monocytogenes excretion was detected. The cow was subjected to antimicrobial therapy, but L....
Tue, 05/14/2019 - 17:00
Jiayu Liu, Ibrahem Jasim, Zhenyu Shen, Lu Zhao, Majed Dweik, Shuping Zhang, Mahmoud Almasri An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has...
Tue, 05/14/2019 - 15:38
Abstract The present study was undertaken to detect the occurrence of beta‐lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n=92) and backyard (n=110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87 phenotypically beta‐lactamase producing E. coli, 19 (17·43%), six (...
Tue, 05/14/2019 - 09:32
Eleutherine americana, a well‐known Asian medicinal herb, has been claimed for many biological activities. In this study, potential of E. americana ethanolic extract against adhesion and invasion of Caco‐2 cells by commonly contaminated Campylobacter spp. was evaluated. MIC50 values of E. americana extract against 39 enteritis patient and 26 chicken isolates were 125 µg/ml. Two models were set up...
Mon, 05/13/2019 - 20:00
Fast molecular detection methods benefit from ready-to-run lab-on-a-chip molecular assays with minimum preparation time. Detection efficiency of such methods can improve if multiple targets are detected simult...
Mon, 05/13/2019 - 20:00
The aim of this study is to investigate the antimicrobial synergistic effect against Campylobacter jejuni, a leading foodborne pathogen that causes human gastroenteritis, by cinnamon oil, encapsulated curcumin, and zinc oxide nanoparticles (ZnO NPs). We compared three approaches to study the antimicrobial interactions, including the time-killing method, the fractional inhibitory concentration...
Mon, 05/13/2019 - 17:00
S. Peter Howard, Leandro F. Estrozi, Quentin Bertrand, Carlos Contreras-Martel, Timothy Strozen, Viviana Job, Alexandre Martins, Daphna Fenel, Guy Schoehn, Andréa Dessen The type II secretion system (T2SS) is a cell envelope-spanning macromolecular complex that is prevalent in Gram-negative bacterial species. It serves as the predominant virulence mechanism of many bacteria including those of...
Mon, 05/13/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 916-925, June 2019.
Mon, 05/13/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 916-925, June 2019.
Mon, 05/13/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 916-925, June 2019.
Mon, 05/13/2019 - 01:14
Available online 11 May 2019 Author(s): Débora Rodrigues Silveira, Janaina Viana da Rosa, Kauana Kaefer, Luiz Gustavo Bach, Amanda de Oliveira Barbosa, Cláudio Dias TimmAbstractThe present study aimed to investigate the presence of Vibrio vulnificus in fish captured at the Lagoa dos Patos estuary (RS, Brazil), to establish a correlation between bacterial biofilm formation and sublethal stress,...
Mon, 05/13/2019 - 01:11
Available online 11 May 2019 Author(s): Lyddia Wilson, Kanwal Mohammad Iqbal, Terrie Simmons-Ehrhardt, Massimo F. Bertino, Muhammed Raza Shah, Vamsi K. Yadavalli, Christopher J. EhrhardtAbstractGaps in our understanding of the natural ecology and survival mechanisms of pathogenic bacteria in complex microenvironments such as soil typically occur due to the difficulty in characterizing biochemical...
Sun, 05/12/2019 - 01:14
Available online 9 May 2019 Author(s): Feifei Xu, Bei Wang, Chen Hong, Shabuerbieke Telebielaigen, John Nsor-Atindana, Yuqing Duan, Fang ZhongAbstractThe effect of a spiral continuous flow-through pulsed light (PL) system on the inactivation of Escherichia coli in red grape juice was evaluated. The grape juice was treated with different numbers of pulses, intensities of PL and liquid flow rates....
Sun, 05/12/2019 - 01:14
Publication date: August 2019Source: LWT, Volume 111Author(s): Chiara Rossi, Clemencia Chaves-López, Sonja Smole Možina, Carla Di Mattia, Stefania Scuota, Ida Luzzi, Teja Jenič, Antonello Paparella, Annalisa SerioAbstractSalmonella is a foodborne pathogen able to adhere and persist on biotic and abiotic surfaces, including vegetables, which are even more linked to foodborne outbreaks. In this...
Sun, 05/12/2019 - 01:14
Publication date: Available online 9 May 2019Source: LWTAuthor(s): Karina Oliveira Lima, Camila da Costa de Quadros, Meritaine da Rocha, José Thalles Jocelino Gomes de Lacerda, Maria Aparecida Juliano, Meriellen Dias, Maria Anita Mendes, Carlos PrenticeAbstractProtein hydrolyzates of Stripped weakfish (Cynoscion guatucupa) industrial byproducts were prepared, and their antimicrobial and...
Sun, 05/12/2019 - 01:14
Publication date: Available online 9 May 2019Source: LWTAuthor(s): Yuanyuan Pan, Jun-Hu Cheng, Xiaoye Lv, Da-Wen SunAbstractA study was carried out to detect and assess possible sublethal injury and inactivation kinetics of Listeria monocytogenes suspended in phosphate-buffered saline (0.01 M PBS, pH = 7.4) by Ar/O2 plasma treatment. Results showed that both the germicidal effect and proportion...
Sun, 05/12/2019 - 01:13
Available online 11 May 2019 Author(s): Maricarmen Iñiguez-Moreno, Melesio Gutiérrez-Lomelí, María Guadalupe Avila-NovoaAbstractPathogens and spoilage microorganisms can develop multispecies biofilms on food contact surfaces; however, few studies have been focused on evaluated mixed biofilms of these microorganisms. Therefore this study investigated the biofilm development by pathogenic (Bacillus...
Sun, 05/12/2019 - 00:00
Salmonella is a type of serious foodborne pathogenic bacterium that threatens the food safety worldwide. In this study, a fluorescence probe-based recombinase polymerase amplification analytical method (exo RPA) was developed for the sensitive and specific detection of Salmonella enterica (S. enterica). The primers and probe for this method were designed based on the iroB gene...
Sat, 05/11/2019 - 10:14
Abstract Aim Intrauterine growth retardation (IUGR) is a prevalent problem in mammals. The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets. Methods and Results We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21, and 28 days of age using 16S rRNA sequencing. The results showed that IUGR piglets had a decreased...
Sat, 05/11/2019 - 01:13
Available online 9 May 2019 Author(s): M.F. Peruzy, N. Murru, Z. Yu, P.-J. Kerkhof, B. Neola, M. Joossens, Y.T.R. Proroga, K. HoufAbstractWild boars (Sus scrofa) are the most widely distributed large mammals and recent increase in consumption of wild boar meat urges the need of microbiological quality criteria. The aim of the study was to characterize the initial bacterial contamination on...
Fri, 05/10/2019 - 17:00
Marija Miljkovic, Sofija Jovanovic, Paula M. O’Connor, Nemanja Mirkovic, Branko Jovcic, Brankica Filipic, Miroslav Dinic, David John Studholme, Djordje Fira, Paul D. Cotter, Milan Kojic Bacteria active against multi-drug resistant pathogens, isolated direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and...
Fri, 05/10/2019 - 05:04
Abstract Biofilms of Lactobacillus plantarum and Listeria innocua were grown under single‐ and mixed‐culture conditions in a bioreactor with either half‐strength brain heart infusion (BHI) medium or BHI medium with sodium dichloroisocyanurate (150 ppm) for 24 hr. A quantitative approach using plate counts and fluorescence in situ hybridization (FISH) was applied, representing the culturable and...
Fri, 05/10/2019 - 05:04
Abstract Biofilms of Lactobacillus plantarum and Listeria innocua were grown under single‐ and mixed‐culture conditions in a bioreactor with either half‐strength brain heart infusion (BHI) medium or BHI medium with sodium dichloroisocyanurate (150 ppm) for 24 hr. A quantitative approach using plate counts and fluorescence in situ hybridization (FISH) was applied, representing the culturable and...
Fri, 05/10/2019 - 05:04
Abstract Vibrio parahaemolyticus is the leading cause of illness associated with seafood consumption globally. In this study, we examined 136 V. parahaemolyticus isolates from Ningbo, China for antimicrobial resistance phenotypes, genotypes, virulence factor genes (tdh, trh, T3SS) and molecular typing. Carbenicillin resistance (100.0%) was the most prevalent in all isolates followed by...
Fri, 05/10/2019 - 05:04
Abstract Vibrio parahaemolyticus is the leading cause of illness associated with seafood consumption globally. In this study, we examined 136 V. parahaemolyticus isolates from Ningbo, China for antimicrobial resistance phenotypes, genotypes, virulence factor genes (tdh, trh, T3SS) and molecular typing. Carbenicillin resistance (100.0%) was the most prevalent in all isolates followed by...
Fri, 05/10/2019 - 05:03
Abstract Cantaloupes contaminated with Listeria and Salmonella have caused large scale of foodborne illness outbreaks in recent years. Prior research has shown that heat treatment can be used to reduce microbial contaminates on fruit surfaces. However, no study has evaluated the use of kitchen steamers for inactivating foodborne pathogens on cantaloupes. The purpose of this study was to test the...
Fri, 05/10/2019 - 05:03
Abstract Cantaloupes contaminated with Listeria and Salmonella have caused large scale of foodborne illness outbreaks in recent years. Prior research has shown that heat treatment can be used to reduce microbial contaminates on fruit surfaces. However, no study has evaluated the use of kitchen steamers for inactivating foodborne pathogens on cantaloupes. The purpose of this study was to test the...
Fri, 05/10/2019 - 01:15
Publication date: Available online 9 May 2019Source: LWTAuthor(s): Ângelo Luís, Luísa Pereira, Fernanda Domingues, Ana RamosAbstractAntioxidant and antibacterial edible films based on carboxymethyl xylan with licorice essential oil incorporated were successfully developed. The films were prepared by the casting method and characterized using FTIR. The grammage, thickness, contact angle,...
Thu, 05/09/2019 - 10:29
Campylobacter jejuni helical shape is key to its pathogenesis. Under stress, C. jejuni alters its shape from helical to coccoid. This study characterized C. jejuni bacterial cell wall peptidoglycan changes occurring during coccoid formation and identified a role for the peptidoglycan hydrolases Pgp1 and previously uncharacterized AmiA in the process. In addition, we determined that coccoid C....
Thu, 05/09/2019 - 09:25
Abstract Thyme essential oil‐loaded microcapsules (TMS) were fabricated using natural polysaccharide chitosan (CS) and sodium alginate (SA) as the shell material via the method of layer‐by‐layer (LBL) assembly. The accumulated release rates of thyme oil and microcapsules at 4 °C were 42.50% and 10.16%, respectively. After heating at 100 °C for 5 hr, the release rate of the 0, 2, 4, 6 layers...
Thu, 05/09/2019 - 01:16
Available online 8 May 2019 Author(s): Jéssica B. Portela, Pablo T. Coimbra, Leandro P. Cappato, Verônica O. Alvarenga, Rodrigo B.A. Oliveira, Karen S. Pereira, Denise.R.P. Azeredo, Anderson S. Sant’ Ana, Janaina S. Nascimento, Adriano G. CruzAbstractThis study aimed to study the behavior of Salmonella submitted to domestic microwave through the use of predictive microbiology. The results showed...
Wed, 05/08/2019 - 20:00
As WGS is increasingly used by food industry to characterize pathogen isolates, users are challenged by the variety of analysis approaches available, ranging from methods that require extensive bioinformatics expertise to commercial software packages. This study aimed to assess the impact of analysis pipelines (i.e., different hqSNP pipelines, a cg/wgMLST pipeline) and the reference genome...
Wed, 05/08/2019 - 01:17
Available online 7 May 2019 Author(s): Alexandre José Cichoski, Diego Rafael Martins Flores, Cristiano Ragagnin De Menezes, Eduardo Jacob-Lopes, Leila Queiroz Zepka, Roger Wagner, Juliano Smanioto Barin, Érico Marlon de Moraes Flores, Marilda da Cruz Fernandes, Paulo Cezar Bastianello CampagnolAbstractPre-chilling leads to a temperature decline of the pre-rigor muscle of poultry carcasses, and a...
Wed, 05/08/2019 - 01:17
Available online 4 May 2019 Author(s): B. Ujvári, L. Makrai, T. MagyarAbstractThis study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA) sequence of Pasteurella multocida isolates (n = 180) from different locations in Hungary, from various host species, including humans. When combining capsular...
Wed, 05/08/2019 - 01:15
Available online 6 May 2019 Author(s): Rafael D. Chaves, Zafeiro Aspridou, Anderson S. Sant'Ana, Konstantinos P. KoutsoumanisAbstractThe present work investigates the effect of chlorine stress on the subsequent growth behavior of individual Salmonella cells. A time-lapse microscopy method was used which allowed to evaluate the effect of chlorine on the division times of Salmonella individual...
Wed, 05/08/2019 - 01:15
Publication date: August 2019Source: LWT, Volume 110Author(s): Priti Mudgil, Lina S. Omar, Hina Kamal, Bhanu Priya Kilari, Sajid MaqsoodAbstractThe present study explored the bioactive properties (antioxidant, antihaemolytic and antibacterial properties) of hydrolysates produced from quinoa and amaranth protein isolates. Various hydrolysates were produced using different enzymes (Bromelain,...

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