You are here

Bacterial pathogens

Sat, 04/11/2015 - 01:26
Cary Pirone-Davies, Maria Hoffmann, Richard J. Roberts, Tim Muruvanda, Ruth E. Timme, Errol Strain, Yan Luo, Justin Payne, Khai Luong, Yi Song, Yu-Chih Tsai, Matthew Boitano, Tyson A. Clark, Jonas Korlach, Peter S. Evans, Marc W. Allard The methylation of DNA bases plays an important role in numerous biological processes including development, gene expression, and DNA replication. Salmonella is...
Sat, 04/11/2015 - 01:26
Salmonella enterica serovar Enteritidis is a major foodborne pathogen in the United States, causing gastroenteritis in humans, primarily through consumption of contaminated eggs. Chickens are the reservoir host of S. Enteritidis. In layer hens, S. Enteritidis colonizes the intestine and migrates to various organs, including the oviduct, leading to egg contamination. This study investigated the...
Sat, 04/11/2015 - 01:26
Fifty-nine monophasic Salmonella enterica serovar Typhimurium isolates, collected in Belgium during the period from 2008 to 2011, have been serotyped as 4,[5]:i:– and shown to harbor an fljB coding sequence. The genetic differences between these strains and phenotypically biphasic Salmonella Typhimurium were analyzed through PCR and DNA sequencing. Genetic alterations in the fljB promoter...
Sat, 04/11/2015 - 01:26
Methylmercury is a potent neurotoxin that is produced by anaerobic microorganisms from inorganic mercury by a recently discovered pathway. A two-gene cluster, consisting of hgcA and hgcB, encodes two of the proteins essential for this activity. hgcA encodes a corrinoid protein with a strictly conserved cysteine proposed to be the ligand for cobalt in the corrinoid cofactor, whereas hgcB encodes a...
Sat, 04/11/2015 - 01:26
Vibrio cholerae is a severe human pathogen and a frequent member of aquatic ecosystems. Quantification of V. cholerae in environmental water samples is therefore fundamental for ecological studies and health risk assessment. Beside time-consuming cultivation techniques, quantitative PCR (qPCR) has the potential to provide reliable quantitative data and offers the opportunity to quantify multiple...
Sat, 04/11/2015 - 01:26
Listeriolysin O (LLO), an essential virulence determinant of Listeria monocytogenes, is a pore-forming toxin whose primary function is to facilitate cytosolic bacterial replication by breaching the phagosomal membranes, which is critical for the pathogen to evade host immune recognition. The critical role of LLO in the virulence of L. monocytogenes renders it an ideal target for designing novel...
Sat, 04/11/2015 - 01:26
Publication date: 15 October 2015 , Volume 185 Author(s): Yi Zhao , Mingshun Chen , Zhengang Zhao , Shujuan Yu Sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth. In the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol. The sugarcane bagasse...
Sat, 04/11/2015 - 01:26
Publication date: 15 October 2015 , Volume 185 Author(s): D. James Bound , Pushpa S. Murthy , P. Srinivas Essential oils and their oxygenated terpene constituents possess potent antimicrobial properties. In the present study, a facile synthetic route to the 2,3-dideoxy 1- O -glucosides of important phenols and terpene alcohols in excellent yields (85–96%) has been delineated. Studies on...
Fri, 04/10/2015 - 01:21
Publication date: 16 July 2015 , Volume 205 Author(s): Luís Gustavo Corbellini , Ana Sofia Ribeiro Duarte , Leonardo Víctor de Knegt , Luis Eduardo da Silva , Marisa Cardoso , Maarten Nauta In microbiological surveys, false negative results in detection tests precluding the enumeration by MPN may occur. The objective of this study was to illustrate the impact of screening test failure...
Fri, 04/10/2015 - 01:21
As direct food contact material, wood is subject to European regulation no. 1935/2004 of 27 October 2004, which specifies that materials intended for safe food contact must not interfere with foodstuff characteristics. In order to comply with this regulation, it is important to provide an efficient recovery method so that the microbial load on wooden surfaces in direct...
Fri, 04/10/2015 - 01:21
e-First Articles. Microorganisms can produce species-specific microbial volatile organic compounds (MVOCs), or odor compounds, which can be characterized by odor fingerprinting. The objective of this study was to characterize the odor fingerprint of Listeria monocytogenes. Solid-phase microextraction – gas chromatography – mass spectrometry (SPME–GC–MS) and electronic nose (E-nose) were used to...
Thu, 04/09/2015 - 01:28
Meer T. Alam, Thomas A. Weppelmann, Ira Longini, Valery Madsen Beau De Rochars, John Glenn Morris, Afsar Ali Since the identification of the first cholera case in 2010, the disease has spread in epidemic form throughout the island nation of Haiti; as of 2014, about 700,000 cholera cases have been reported, with over 8,000 deaths. While case numbers have declined, the more fundamental question of...
Thu, 04/09/2015 - 01:28
Publication date: October 2015 , Volume 56 Author(s): Haiying Cui , Chengting Zhao , Lin Lin In this study, the antibacterial activities of Clove oil and liposome-encapsulated Clove oil were investigated. First, the antibacterial activity of Clove oil demonstrated that the essential oil exhibited favorable antimicrobial activity for both Escherichia coli and Staphylococcus aureus . However...
Wed, 04/08/2015 - 06:20
RecQ helicases unwind remarkably diverse DNA structures as key components of many cellular processes. How RecQ enzymes accommodate different substrates in a unified mechanism that couples ATP hydrolysis to DNA unwinding is unknown. Here, the X-ray crystal structure of the Cronobacter sakazakii RecQ catalytic core domain bound to duplex DNA...
Wed, 04/08/2015 - 01:23
Publication date: August 2015 , Volume 106 Author(s): Ricardo César Tavares Carvalho , Leone Vinícius Furlanetto , Fernanda Harumy Maruyama , Cristina Pires de Araújo , Sílvia Letícia Bomfim Barros , Carlos Alberto do Nascimento Ramos , Valéria Dutra , Flábio Ribeiro de Araújo , Vânia Margaret Flosi Paschoalin , Luciano Nakazato , Eduardo Eustáquio de Souza Figueiredo Bovine...
Wed, 04/08/2015 - 01:23
Publication date: 2 July 2015 , Volume 204 Author(s): C. Plaza-Rodríguez , C. Thoens , A. Falenski , A.A. Weiser , B. Appel , A. Kaesbohrer , M. Filter Transferring the knowledge of predictive microbiology into real world food manufacturing applications is still a major challenge for the whole food safety modelling community. To facilitate this process, a strategy for creating open,...
Wed, 04/08/2015 - 01:23
Publication date: 2 July 2015 , Volume 204 Author(s): Veerachandra K. Yemmireddy , Yen-Con Hung The purpose of this study was to determine the effect of food processing organic matter on photocatalytic bactericidal activity of titanium dioxide (TiO 2 ) nanoparticles (NPs). Produce and meat processing wash solutions were prepared using romaine lettuce and ground beef samples. Physico-...
Wed, 04/08/2015 - 01:23
Publication date: 2 July 2015 , Volume 204 Author(s): Junping Wang , Xinfang Xie , Jinsong Feng , Jessica C. Chen , Xin-jun Du , Jiangzhao Luo , Xiaonan Lu , Shuo Wang Listeria monocytogenes is a facultatively anaerobic, Gram-positive, rod-shape foodborne bacterium causing invasive infection, listeriosis, in susceptible populations. Rapid and high-throughput detection of this...
Wed, 04/08/2015 - 01:23
Publication date: 2 July 2015 , Volume 204 Author(s): Nina Bjerre Østergaard , Lasse Engbo Christiansen , Paw Dalgaard A stochastic model was developed for simultaneous growth of low numbers of Listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. During more than two years, different batches of cottage cheese with aroma...
Wed, 04/08/2015 - 01:23
Publication date: October 2015 , Volume 56 Author(s): Ravirajsinh Jadeja , Marlene E. Janes , Janet G. Simonson The current study was designed to develop an antibody based chromatographic lateral flow detection device (LFD) for rapid identification of Vibrio vulnificus from oyster homogenate. Anti-H monoclonal antibodies against V. vulnificus were prepared and used in the development of...
Tue, 04/07/2015 - 01:28
The biochemistry and microbial ecology of 2 similar types of waterymul) kimchi, containing sliced and unsliced radish and vegetables (nabak and dongchimi, respectively), were investigated. Samples from kimchi were fermented at 4, 10, and 20 °C were analyzed by plating on differential and selective media, high-performance liquid chromatography, and high-throughput DNA sequencing of 16S rDNA. Nabak...
Tue, 04/07/2015 - 01:28
Publication date: 15 May 2015 , Volume 177, Issues 1–2 Author(s): Amanda J. Shapiro , Katrina L. Bosward , Jane Heller , Jacqueline M. Norris The seroprevalence of Coxiella burnetii ( C. burnetii ) in cats in eastern Australia is unknown, and the risk of transmission from cats to humans is undetermined. This study aimed to determine the exposure of cats to C. burnetii in four distinct cat...
Tue, 04/07/2015 - 01:28
Publication date: October 2015 , Volume 56 Author(s): Anthony Pavic , Julian M. Cox , Jeremy W. Chenu In Australia, increased consumer demand for chicken meat and socioeconomic pressures have forced poultry operations to extend plant operating time with delayed spin-chiller emptying and cleaning to maximise water use efficiency. There are concerns that these measures may compromise the...
Tue, 04/07/2015 - 01:28
Publication date: 15 May 2015 , Volume 177, Issues 1–2 Author(s): Christiane Cuny , Mohamed Abdelbary , Franziska Layer , Guido Werner , Wolfgang Witte The immune evasion gene cluster (IEC) is typical for Staphylococcus aureus isolated from humans but is usually absent in S. aureus isolated from animals. Previous studies have shown that methicillin resistant S. aureus (MRSA) CC398...
Tue, 04/07/2015 - 01:28
The count of Listeria monocytogenes was determinedbefore and after heat treatment, in 200 samples of dumplings of 9 brands and with different types of stuffing. Analyses were conducted according to ISO 11290–1 standard and with real-time PCR method. The highest count of L. monocytogenes was found in meat dumplings (102 to 104 CFU/g), whereas products with white cheese-potato stuffing and...
Mon, 04/06/2015 - 01:18
Biofilm of Cronobacter sakazakii on food contact surfaces is the important source of persistent contamination in powdered infant formula. This study aimed to assess the effects of Ca2+ and Mg2+ on biofilm formation. Results indicated that the biofilm-forming ability was strain specific and divalent cation concentrations dependent. Most of C. sakazakii formed the highest amount of biomass at 1.50...
Mon, 04/06/2015 - 01:18
Escherichia coli is one of the foodborne pathogens associated with several cases of human sickness. Duck meat is an excellent source of animal-derived high quality proteins. This study was undertaken to investigate the possible transmission of diarrheagenic E. coli from consumption of duck meat and giblets. Additionally, expression of some virulence-associated genes in the isolated E. coli...
Fri, 04/03/2015 - 06:20
Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in &...
Fri, 04/03/2015 - 06:20
In order to elucidate the main predictors of Vibrio cholerae dynamics and to estimate the risk of V.cholerae related diseases, a recently developed direct detection approach based on FISH and solid phase cytometry (CARD-FISH/SPC) was applied in comparison to cultivation for water samples from the lake Neusiedler See, Austria and three shallow alkaline lakes over a period of 20 months. V.cholerae...
Fri, 04/03/2015 - 01:33
Foodborne Pathogens and Disease Apr 2015, Vol. 12, No. 4: 323-326.
Fri, 04/03/2015 - 01:33
Publication date: October 2015 , Volume 56 Author(s): Carlos Alonso-Calleja , Emilia Guerrero-Ramos , Alicia Alonso-Hernando , Rosa Capita The objective of this paper was to determine whether Escherichia coli ATCC 12806 can adapt to growth in increasing concentrations of three biocides commonly used in food processing facilities (trisodium phosphate [TSP], sodium nitrite [SNI] or sodium...
Thu, 04/02/2015 - 01:21
Publication date: October 2015 , Volume 56 Author(s): María Ruiz-Rico , Cristina Fuentes , Édgar Pérez-Esteve , Ana I. Jiménez-Belenguer , Amparo Quiles , María D. Marcos , Ramón Martínez-Máñez , José M. Barat Development of nanotechnologies to improve the functionality of natural antimicrobials for food applications has received much attention in recent years. Mesoporous silica...
Thu, 04/02/2015 - 01:21
The microaerophilic food-borne pathogen Campylobacter jejuni uses complex cytochrome-rich respiratory chains for growth and host colonization. Cytochrome c biogenesis requires haem ligation to reduced apocytochrome cysteines, catalysed by the cytochrome c synthase, CcsBA. While ccsBA could not be deleted, we showed that the thiol reductase DsbD and the CcsX homologue Cj1207 are involved in, but...
Thu, 04/02/2015 - 01:21
Publication date: 2 July 2015 , Volume 204 Author(s): Yun Jiang , Shuang Yin , Edward G. Dudley , Catherine N. Cutter Shiga toxin-producing Escherichia coli (STEC) strains, including those of O157:H7 and the “big six” serogroups (i.e., O26, O45, O103, O111, O121, and O145) are food-borne pathogens that pose a serious health threat to humans. Ruminants, especially cattle, are a major...
Fri, 01/30/2015 - 01:18
One unreported case of extended-spectrum-beta-lactamase (ESBL)-producing Salmonella enterica serovar Typhi was identified, whole-genome sequence typed, among other analyses, and compared to other available genomes of S. Typhi. The reported strain was similar to a previously published strain harboring blaSHV-12 from the Philippines and likely part of an undetected outbreak, the first of ESBL-...
Fri, 01/30/2015 - 01:18
We encountered a rare case of severe fatal infection in a 70-year-old woman due to Campylobacter upsaliensis, identified by PCR amplification and sequencing analysis of the 16S rRNA gene using DNA extracted from the isolates. To our knowledge, fatal sepsis due to this organism has never been described to date.
Fri, 01/30/2015 - 01:18
An unusual increase in the number of Campylobacter concisus isolates found in stool cultures provoked an outbreak investigation at Bern University Hospital. No epidemiological links were found between the cases, and the Campylobacter isolates were clonally unrelated. A change in culture conditions to a hydrogen-rich atmosphere enhancing growth of C. concisus was deemed responsible for this pseudo...

Pages