Bacterial pathogens

Fri, 02/07/2020 - 04:07
Author(s): Haiyan Zeng, Chengsi Li, Na Ling, Jumei Zhang, Moutong Chen, Tao Lei, Shi Wu, Xiaojuan Yang, Dandan Luo, Yu Ding, Juan Wang, Shuhong Zhang, Qingping WuAbstractCronobacter spp. are important foodborne pathogens that infections occur in all age groups, especially cause serious life-threatening diseases in infants. This study aimed to acquire data on Cronobacter spp. contamination of meat...
Fri, 02/07/2020 - 04:07
Author(s): Danielle M. Schultze, Ricardo Couto, Feral Temelli, Lynn M. McMullen, Michael GänzleAbstractLow water activity (aW) foods permit the survival of low-infectious dose pathogens including Escherichia coli and Salmonella. Desiccation of non-heat resistant E. coli and Salmonella enterica increases their heat resistance; therefore, alternative methods are necessary to ensure the safety of...
Fri, 02/07/2020 - 04:04
Author(s): Robert Webb, Patrick M. Wright, Jennifer L. Brown, Janet C. Skerry, Rebecca L. Guernieri, Theresa J. Smith, Christopher Stawicki, Leonard A. SmithAbstractBotulism is an acute neuroparalytic affliction of the motor and autonomic neurons caused by the toxins produced from Clostridium botulinum and related bacterial strains. The botulinum neurotoxins, or BoNTs, consist of a...
Fri, 02/07/2020 - 04:04
LWT
Author(s): Atefeh Sadat Kashfi, Yousef Ramezan, Mohammad Reza KhaniAbstractThis study purpose was to investigate the effect of radiofrequency low pressure cold plasma (LPCP) process by using dried peppermint (Mentha piperita) on the reduction in microbial load, antioxidant activity, total phenolic content, and color. The microbial load of the sample was investigated by infecting it with 106 CFU/g...
Fri, 02/07/2020 - 03:57
The effects associated with fat content (0% to 40%) of pork batter and the positions (center and surface) on both ohmic heating (OH) and water bath heating (WB) treated samples, were investigated. The OH achieved a more uniform temperature distribution in pork batter as compared to WB‐ and OH‐treated samples showed lower cooking loss values for all samples tested. Besides, the thiobarbituric acid...
Fri, 02/07/2020 - 03:53
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Fri, 02/07/2020 - 03:53
Aims Exposure of Listeria monocytogenes to osmotic stress can induce increased resistance to subsequent lethal exposure to cell envelope stressors, such as nisin and bile salts. We wanted to determine if similar cross‐protection phenotypes could occur when L. monocytogenes strains were treated with osmotic stress and exposed to sub‐lethal levels of the cell envelope stressor, bile. Method and...
Fri, 02/07/2020 - 03:50
The current study made an attempt to develop a novel surface plasmon resonance (SPR) sensor using wheat germ agglutinin as a biomolecule for the rapid detection of Listeria monocytogenes. A selective biomolecular interaction of wheat germ agglutinin (WGA) lectin with surface carbohydrate components of L. monocytogenes was used as a biorecognition principle. From the previous studies based on...
Fri, 02/07/2020 - 03:47
The increasing rates of resistance to traditional anti-Salmonella agents have made the treatment of invasive salmonellosis more problematic, which necessitates the search for new antimicrobial compounds. In this study, the action mode of BSN-37, a novel antibacterial peptide (AMP) from bovine spleen neutrophils, was investigated against Salmonella enterica serovar Typhimurium (S. Typhimurium)....
Fri, 02/07/2020 - 03:47
Klebsiella pneumoniae, a common cause of clinical mastitis (CM) in dairy cows, can cause severe clinical symptoms. However, its pathogenicity in the bovine mammary gland is not well understood. Our objectives were to establish an in vitro infection model of K. pneumoniae on bovine mammary epithelial cells (bMEC) to assess (1) cytopathogenicity (adhesive and invasive ability, damage and apoptosis...
Fri, 02/07/2020 - 03:47
Our objective was to assess the enterotoxigenic potential of coagulase-negative staphylococci (CNS) isolated from bovine milk in Poland. We analyzed CNS isolates collected from 133 bovine milk batches from dairy farms in the Western Pomerania district during 2 milking seasons. A total of 163 isolates were screened by multiplex/duplex PCR for the presence of 18 of 25 enterotoxin genes identified...
Fri, 02/07/2020 - 03:47
Cronobacter spp. are important opportunistic foodborne pathogens in powdered infant formula that cause many serious diseases in neonates and infants. In this study, a novel assay based on dual signal amplification strategy was developed by coupling asymmetric tailing PCR (AT-PCR) with rolling circle amplification (RCA) for the detection of Cronobacter spp. in milk. The tailing single-stranded DNA...
Thu, 02/06/2020 - 03:37
Author(s): Sheng-Wei Luo, Zi-Ye Luo, Teng Yan, Kai-Kun Luo, Ping-Hui Feng, Shao-Jun LiuAbstractHepcidin, a multifunctional hormone oligopeptide, not only exhibits a regulatory role in iron metabolism, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-hepcidin was 258 bp and encoded 85 amino acid residues. Tissue-specific analysis revealed that the...
Thu, 02/06/2020 - 03:37
Author(s): KaWang Li, Xiaoli Etienne, Yu-Chun Chiu, Lisa Jones, Hanna Khouryieh, Wentao Jiang, Cangliang ShenAbstractThis study aims to i) evaluate two triple-wash procedures with an H2O2-peroxyacetic-acid mixer (SaniDate-5.0) to improve microbial safety and quality of butternut squashes and ii) determine the feasibility of the triple-wash application in a processing plant. In study I, fresh...
Wed, 02/05/2020 - 03:38
by Franziska Nienhaus, Diana Meemken, Clara Schoneberg, Maria Hartmann, Thomas Kornhoff, Thomas May, Sabrina Heß, Lothar Kreienbrock, Anna Wendt There are growing demands to ensure animal health and, from a broader perspective, animal welfare, especially for farmed animals. In addition to the newly developed welfare assessment protocols, which provide a harmonised method to measure animal health...
Wed, 02/05/2020 - 03:36
Author(s): Xing-Wei Liu, Lin Feng, Wei-Dan Jiang, Pei Wu, De-Min Yang, Ling Tang, Sheng-Yao Kuang, He-Qun Shi, Xiao-Qiu Zhou, Yang LiuAbstractThe present study evaluated the effects of (2-Carboxyethyl)dimethylsulfonium Bromide (Br-DMPT) supplementation on the intestinal immune function and potential mechanisms of on-growing grass carp (Ctenopharyngodon idella) by feeding fish (initial weight 216....
Wed, 02/05/2020 - 03:36
Author(s): Wanxia Sun, Tianmei Sun, Xiang Wang, Qing Liu, Qingli DongAbstractA quantitative probabilistic model was developed to estimate the concentration of Listeria monocytogenes in cooked meat products based on presence/absence data and an assumed zero-inflated distribution, i.e. zero-inflated Poisson (ZIP) or zero-inflated Poisson lognormal (ZIPL) distribution. The performance of these two...
Wed, 02/05/2020 - 03:33
Author(s): Jay Prakash Yadav, Satya Veer Singh Malik, Pankaj Dhaka, Manesh Kumar, Sirsant Bhoomika, Diksha Gourkhede, Sukhadeo B. Barbuddhe, Deepak B. RawoolAbstractThe in-house developed DnaK and Com1 synthetic peptide-based Latex Agglutination Tests (LATs) were comparatively evaluated with commercial indirect-ELISA kit, to provide a rapid, economical and onsite field applicable test for...
Wed, 02/05/2020 - 03:30
The study assessed the antibacterial and antifungal effect of Moringa oleifera seed marinade on smoked Clarias gariepinus stored at ambient temperature for 15 days. The optimum effective concentration of moringa seed marinade that acts as antibacterial was found best to be 11.2 g/kg of fish moringa marinade. While 12.08 g/kg exhibited the highest anti‐yeast effect in Clarias gariepinus and 10.6 g...
Wed, 02/05/2020 - 03:27
The objective of this study was to determine whether seeds of Brassica oleracea var. italica (i.e. broccoli, an edible plant) produce defensins that inhibit phytopathogenic fungi and pathogenic bacteria of clinical significance. Crude extracts obtained from broccoli seeds were fractioned by molecular exclusion techniques and analyzed by liquid chromatography-high-resolution mass spectrometry. Two...
Wed, 02/05/2020 - 03:26
Fast response to environmental changes plays a key role in the transmission and pathogenesis of Yersinia enterocolitica. Osmoregulated periplasmic glucans (OPGs) are known to be involved in environmental perception of several Enterobacteriaceae pathogens; however, the biological function of OPGs in Y. enterocolitica is still unclear. In this study, we investigated the role of OPGs in Y....
Wed, 02/05/2020 - 03:26
Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific...
Wed, 02/05/2020 - 03:26
Author(s): Rafik Balti, Mohamed Ben Mansour, Nourhene Zayoud, Romain Le Balc'h, Nicolas Brodu, Abdellah Arhaliass, Anthony Massé Active edible coatings from microalgal exopolysaccharides (EPS) enriched with different concentrations of red seaweed extract (RSE) (0.5, 1 and 1.5% (w/v)) were developed and their effects on shrimp quality with refrigerated storage (4 ± 1 °C) were studied over a period...
Tue, 02/04/2020 - 04:10
Prodigiosin, a secondary metabolite produced by Serratia marcescens, has attracted attention due to its immunosuppressive, antimicrobial, and anticancer properties. However, information on the regulatory mechanism behind prodigiosin biosynthesis in S. marcescens remains limited. In this work, a prodigiosin-hyperproducing strain with the BVG90_22495 gene disrupted (ZK66) was selected from a...
Tue, 02/04/2020 - 04:10
The gut-lymph node axis is a critical player in the symbiotic relationship between gut microbiota and the host. However, little is known about the impact of diet-related bacterial shifts in the gut lumen on bacterial translocation into lymph nodes. Here, we (i) characterized changes in the viable microbiota composition along the ileal digesta-mucosa-lymph node axis and (ii) examined the effect of...
Tue, 02/04/2020 - 04:10
Some chlorine-resistant Escherichia coli isolates harbor the locus of heat resistance (LHR), a genomic island conferring heat resistance. In this study, the protective effect of the LHR for cells challenged by chlorine and oxidative stress was quantified. Cloning of the LHR protected against NaClO (32 mM; 5 min), H2O2 (120 mM; 5 min), and peroxyacetic acid (105 mg/liter; 5 min) but not against 5....
Tue, 02/04/2020 - 04:06
Author(s): Tatiane Milkievicz, Vinicius Badia, Vanessa Barreira Souza, Daniel Angelo Longhi, Alessandro Cazonatto Galvão, Weber da Silva RobazzaAbstractThe objective of this study was to develop and validate a model to predict the growth of Salmonella in chicken meat under different isothermal and dynamic temperature conditions. Three different primary and secondary models and combinations were...
Tue, 02/04/2020 - 03:44
Synthetic dyes, generally resistant, toxic and carcinogenic presents a substantial risk to the environment and health of human. The present study was aimed to decolourize a dye mixture (Evans blue and brilliant green) by selected bacterial strains cultivated at different growth conditions (e.g. unmodified, correction of pH value and supplementation with nutrients). The bacterial strains used as...
Tue, 02/04/2020 - 03:42
The polymyxin antibiotic colistin has been used in decades for treatment and prevention of infectious diseases in livestock. Nowadays, it is even considered as last-line treatment option for severe human infections caused by multidrug- and carbapenem-resistant Gram-negative bacteria. Therefore, the discovery of plasmid-mediated mobile colistin resistance (mcr) genes raised major public health...
Tue, 02/04/2020 - 03:42
Resistance to antibiotics is one of the most relevant public health concerns in the world. Aquatic environments play an important role because they are reservoirs for antibiotic resistance genes and antibiotic-resistant strains, contributing to the spread of resistance. The present study investigated the resistome in Lake Bolonha (three sampling sites) in the Amazon region using a metagenomics...
Tue, 02/04/2020 - 03:42
Botulinum toxin A (BTX-A) is a powerful neurotoxin with long-lasting activity that blocks muscle contractions. In addition to effects on neuromuscular junctions, BTX-A also plays a role in sensory feedback loops, suggesting the potentiality for pain relief. Although the only approved indications for BTX-A in the bladder are neurogenic detrusor overactivity and refractory overactive bladder, BTX-A...
Mon, 02/03/2020 - 03:34
Author(s): Yan-li Su, Guang-hua Wang, Jing-jing Wang, Bing Xie, Qin-qin Gu, Dong-fang Hao, Hong-mei Liu, Min ZhangAbstractAt present, several reports have indicated that the C-terminal peptides of tissue factor pathway inhibitor 1 (TFPI-1) were active antibacterial peptides. However, the functions of TFPI-1 C-terminal peptides in teleost are still very limited. In this study, a C-terminal peptide...
Mon, 02/03/2020 - 03:34
Author(s): Reza Roohi, Seyed Mohammad Bagher HashemiAbstractThe coupled 3D and transient form of heat and Maxwell's equations were solved to determine the temperature distribution within a carrot slice in the microwave cavity during pasteurization as a clean and energy saving process. To precisely simulate the turning of the sample, the dissipated thermal energy was applied to the carrot as a...
Mon, 02/03/2020 - 03:33
Author(s): Alonzo A. Gabriel, Cecile Leah T. Bayaga, Eiric A. Magallanes, Richard Paolo M. Aba, Karen May N. TanguiligAbstractThis study was conducted to address the dearth in works that simultaneously compare the growth and inactivation behaviors of selected pathogens in different milk products. In worst-case scenarios where hygienic practices are absent and heavy microbiological contaminations...
Mon, 02/03/2020 - 03:32
Author(s): Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Junsup Lim, Jinsung Choi, Shin Young ParkAbstractThis study investigated the effects of dielectric barrier discharge (DBD) plasma against Staphylococcus aureus and Bacillus cereus (initial inoculum of 8-9 log10CFU/mL) on the surface of dried blackmouth angler, and the physicochemical [pH, Hunter colors, thiobarbituric acid reactive...
Mon, 02/03/2020 - 03:32
Author(s): Solange Aka, Bedis Dridi, Alexandre Bolotin, Elysée Armel Yapo, Marina Koussemon-Camara, Bassirou Bonfoh, Pierre RenaultAbstractThe present study aimed to characterize lactic acid bacteria involved in the different processing steps of tchapalo, a traditional Ivoirian beverage, for their potential application as starter cultures in food and beverages. Lactic acid bacteria (LAB) were...
Mon, 02/03/2020 - 03:25
CTX-MSHVplasmid transferconjugationenteric pathogensantibiotic resistance gastroenteritis
Mon, 02/03/2020 - 03:25
Plazomicin (ACHN-490) is a novel parenteral aminoglycoside developed to target multidrug-resistant Enterobacteriaceae. It has recently been approved by the Food and Drug Administration for the management of complicated urinary tract infections and pyelonephritis caused by susceptible organisms. When compared with meropenem, plazomicin was not inferior. The adverse-event profile for plazomicin was...
Sun, 02/02/2020 - 03:35
Author(s): Duleeka P. Kuruwita, Xiuping Jiang, Duncan Darby, Julia L. Sharp, Angela M. FraserAbstractAn estimated 179 million cases of acute gastroenteritis (AGE) occur each year in the United States. Escherichia coli O157:H7 and Listeria monocytogenes, common etiologic agents of AGE, both have low-infectious doses and high environmental resistance. Surfaces contaminated with either pathogen,...
Sun, 02/02/2020 - 03:32
Author(s): Triveni Kalleshamurthy, Somy Skariah, Yashaswini Rathore, Kavana D. Ramanjinappa, Chaitra Nagaraj, Bibek Ranjan Shome, Habibur Rahman, Nagendra Nath Barman, Rajeswari ShomeAbstractBrucellosis is an important zoonosis that constitutes a serious public health hazard which is caused by a bacterium belonging to the genus Brucella. In the present study, two highly specific serological tests...
Sun, 02/02/2020 - 03:31
LWT
Author(s): Hafiz Abdul Rasheed, Tuhanguli Tuoheti, Yongzhu Zhang, Azi Fidelis, Mekonen Tekliye, Mingsheng DongAbstractIn current study, a novel bacteriocin LF-BZ532 was produced by Lactobacillus fermentum BZ532 that was isolated from Chinese fermented cereal drink (locally called bozai) and identified by 16s rDNA sequencing. The bacteriocin LF-BZ532 was purified by ammonium sulfate precipitation...
Sun, 02/02/2020 - 03:31
LWT
Author(s): Stefani Machado Lopes, Eduardo César TondoAbstractSpaghetti alla carbonara is a traditional Italian dish, which the sauce made of raw egg yolks is heated using only the heat of cooked pasta. Concerns about the safety of this preparation have been raised due the possibility of egg yolks be contaminated by Salmonella and the heat treatment may not be sufficient for total Salmonella...
Sun, 02/02/2020 - 03:30
Durum wheat (Triticum durum Desf.) has potential as an adjunct in brewing given its agronomic, chemical and technological properties. The aim of this work were to identify the cultivable microflora and evaluate the technological quality of the durum wheat variety ‘Senatore Cappelli’ grown and used by a craft brewery in Sardinia, Italy. The isolated bacterial strains were mainly rhizospheric (...
Sat, 02/01/2020 - 04:11
by Samantha Erwin, Derek M. Foster, Megan E. Jacob, Mark G. Papich, Cristina Lanzas Antimicrobial drugs administered systemically may cause the emergence and dissemination of antimicrobial resistance among enteric bacteria. To develop logical, research-based recommendations for food animal veterinarians, we must understand how to maximize antimicrobial drug efficacy while minimizing risk of...
Sat, 02/01/2020 - 04:11
by Roger Pamphlett, Laveniya Satgunaseelan, Stephen Kum Jew, Philip A. Doble, David P. Bishop Objective Exposure to toxic metals such as mercury has been proposed to be a risk factor for the development of breast cancer since some metals can promote genetic mutations and epigenetic changes. We sought to find what toxic metals are present in normal breast tissue and in the tumours of women who had...
Sat, 02/01/2020 - 04:00
Author(s): El-Hajjaji Soundous, Gérard Amaury, De Laubier Juliette, Di Tanna Sybille, Lainé Aurélie, Patz Viviane, Sindic MarianneAbstractButter is a complex matrix characterized by a high fat content. Existing publications on the behavior of Listeria monocytogenes in this type of food reported contrasted results. This study was performed to provide further information and data about raw milk...
Sat, 02/01/2020 - 04:00
Author(s): Laura Settier-Ramírez, Gracia López-Carballo, Rafael Gavara, Pilar Hernández-MuñozAbstractPVOH-based polymer matrices in the form of films were evaluated as carriers of living Lactococcus lactis subsp. Lactis. These lactic acid bacteria are capable of producing nisin, which is an effective antilisterial peptide. A low percentage (1:0.125 w/w) of yeast extract, gelatin, sodium caseinate...
Sat, 02/01/2020 - 03:42
For adaptation to stressful conditions, Mycobacterium tuberculosis (Mtb) is prone to transit to a dormant, non-replicative state, which is believed to be the basis of the latent form of tuberculosis infection. Dormant bacteria persist in the host for a long period without multiplication, cannot be detected from biological samples by microbiological methods, however, their “non-culturable” state...

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