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Bacterial pathogens

Mon, 07/08/2019 - 01:04
Available online 6 July 2019 Author(s): Sonia Lamon, Asmine Bastardo, Domenico Meloni, Simonetta Gianna Consolati, Federica Fois, Gabriella Porcheddu, Vanessa Agus, Margherita Pes, Maria Giovanna Cambula, Anna Mureddu, Jesus L. RomaldeAbstractThe aim of the present study was to investigate the genetic variability of Vibrio parahaemolyticus strains isolated from naturally contaminated...
Mon, 07/08/2019 - 01:04
Available online 5 July 2019 Author(s): Ji-Hoon Kang, Kyung Bin SongAbstractThis study was conducted to investigate the antibacterial activity of the noni fruit extract (NFE) against Listeria monocytogenes (ATCC, 19111 and 19115) and assess its applicability for the washing of fresh-cut produce. Based on the results of the disc diffusion test, L. monocytogenes (ATCC, 19111 and 19115) was...
Mon, 07/08/2019 - 00:00
Abstract The aim of the present study is to predict the shelf life and evaluate the risk profile of an innovative whole soybean curd (WSC). Two main spoilage strains were isolated from spoiled WSC and identified as B. subtilis and B. cereus. The origin analysis confirmed that B. subtilis and B. cereus originated from soybean materials and survived in soybean curd. For microbial...
Sun, 07/07/2019 - 09:19
The combined effect of sodium erythorbate, stable chlorine dioxide, and gellan gum glazing on quality of peeled shrimp was investigated during frozen storage, and compared with traditional water‐ice‐glazing treatment. Data revealed that the growth of total aerobes, Escherichia coli, and Staphylococcus aureus was significantly inhibited in the combined treatment, compared to the water‐ice‐glazing...
Sat, 07/06/2019 - 01:07
Available online 3 July 2019 Author(s): Astrid Caroline Muniz da Silva, Pâmela de Oliveira Pena, Sérgio Bertelli Pflanzer, Maristela da Silva do NascimentoAbstractThe objective of this study was to evaluate the effect of different dry aging temperatures on the behavior of Listeria innocua used as a surrogate of L. monocytogenes. The process was performed in boneless loin pieces for up to 42 days...
Fri, 07/05/2019 - 17:00
Alfred Fillol-Salom, Ahlam Alsaadi, Jorge A. Moura de Sousa, Li Zhong, Kevin R. Foster, Eduardo P. C. Rocha, José R. Penadés, Hanne Ingmer, Jakob Haaber Temperate phages are bacterial viruses that as part of their life cycle reside in the bacterial genome as prophages. They are found in many species including most clinical strains of the human pathogens, Staphylococcus aureus and Salmonella...
Fri, 07/05/2019 - 17:00
Stefan Achtsnicht, Christian Neuendorf, Tobias Faßbender, Greta Nölke, Andreas Offenhäusser, Hans-Joachim Krause, Florian Schröper Cholera is a life-threatening disease caused the cholera toxin (CT) as produced some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic...
Thu, 07/04/2019 - 01:07
Available online 1 July 2019 Author(s): Kristen E. Gibson, Giselle Almeida, Sarah L. Jones, Kacy Wright, Jung Ae LeeAbstractAn estimated 9.4 million illnesses are due to known foodborne pathogens every year. Consumption of fresh produce can be a risk factor for the transmission of foodborne pathogens with the potential for contamination occurring at any node in the farm to fork food pathway....
Thu, 07/04/2019 - 00:00
Toxins, Vol. 11, Pages 392: A Short Peptide Derived from the ZorO Toxin Functions as an Effective Antimicrobial Toxins doi: 10.3390/toxins11070392 Authors: Otsuka Ishikawa Takahashi Masuda Antimicrobial peptides are potential molecules for the development of novel antibiotic agents. The ZorO toxin of a type I toxin–antitoxin system in Escherichia coli O157:H7 is...
Wed, 07/03/2019 - 20:00
Recent research has proven the ability of cold atmospheric plasma (CAP) for assuring food safety. A more flexible and transportable alternative is the use of plasma activated liquids (PAL), which are also known to have antimicrobial properties. However, within the context of food safety, little is known on its potential regarding decontamination. This research therefore focusses on identifying...
Wed, 07/03/2019 - 20:00
With the increase in commercial pig farming, there is a simultaneous increase in the use of antibiotics for prophylaxis as well as therapeutics in China. In this study, we evaluated the prevalence and resistance diversity of Salmonella isolated from faeces of asymptomatic, live and slaughtered pigs. We analyzed 1,732 pig faecal samples collected over eight months, at Henan province of China....
Wed, 07/03/2019 - 17:00
Quail Das, Dion Lepp, Xianhua Yin, Kelly Ross, Jason L. McCallum, Keith Warriner, Massimo F. Marcone, Moussa S. Diarra Non-typhoidal Salmonella enterica serovars continue to be an important food safety issue worldwide. Cranberry (Vaccinium macrocarpon Ait) fruits possess antimicrobial properties due to their various acids and phenolic compounds; however, the underlying mechanism of actions is...
Wed, 07/03/2019 - 15:04
Abstract Efflux mediated multidrug resistance is a well‐known phenomenon facilitated by multidrug resistant (MDR) transporters. One of the approaches to counteract efflux mediated resistance is the use of MDR pump inhibitors, and thus be used in combination with the conventional antibiotics to treat deadly diseases like typhoid fever. We have previously reported that STY4874, an efflux...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Wed, 07/03/2019 - 13:04
Abstract In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and...
Wed, 07/03/2019 - 13:04
Abstract In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and...
Wed, 07/03/2019 - 08:30
Campylobacter is among the four main causes of gastroenteritis worldwide and has increased in both developed and developing countries over the last 10 years. The vast majority of reported Campylobacter infections are caused by Campylobacter jejuni and, to a lesser extent, C. coli; however, the increasing recognition of other emerging Campylobacter pathogens is urgently demanding a better...
Wed, 07/03/2019 - 07:13
Abstract Aims This investigation was undertaken to study the prevalence, enterotoxin gene profile and molecular epidemiology of Aeromonads from various sources of water (182) and fish (173). Methods and Results A total of 116 Aeromonas sp. were isolated, of which 48 (26·37%) were from water and 68 (34·62%) were from fish samples collected from retail markets and fish farms. The Aeromonads were...
Wed, 07/03/2019 - 03:10
Background: Many bacteria transport cellobiose via a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In Listeria monocytogenes, two pairs of soluble PTS components (EIIACel1/EIIBCel1 and EIIACel2/EIIBCel2) and the permease EIICCel1 were suggested to contribute to cellobiose uptake. Interestingly, utilization of several carbohydrates, including cellobiose, strongly represses...
Wed, 07/03/2019 - 01:06
Publication date: October 2019 , Volume 156Author(s): Antonio López-Gómez, María Ros-Chumillas, Vera Antolinos, Laura Buendía-Moreno, Laura Navarro-Segura, María José Sánchez-Martínez, Ginés Benito Martínez-Hernández, Sonia Soto-JoverAbstractApplication of essential oils (EOs) in vapour phase under vacuum conditions may increase the effectiveness of EOs to sanitize plant products. Different...
Tue, 07/02/2019 - 20:00
Research progress into mechanisms of the anaerobe Clostridium perfringens associated diseases has been frustrated by the lack of reliable infection models. Wax moth larvae (Galleria mellonella) have emerged as a viable alternative to other models of infection since they are economic, survive at 37°C and require no specialist equipment. This study aims to establish to what extent G. mellonella...
Tue, 07/02/2019 - 20:00
The aim of this study was to quantify the influence of temperature on pHmin-values of L. monocytogenes as used in cardinal parameter growth models and thereby improve the prediction of growth for this pathogen in food with low pH. Experimental data for L. monocytogenes growth in broth at different pH-values and at different constant temperatures were generated and used to determined pHmin-values...
Tue, 07/02/2019 - 20:00
Salmonella enterica serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in S. Typhimurium had been well defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In the current work, we investigated an oqxAB-positive plasmid pHXY0908 and analyzed its role in S....
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Tue, 07/02/2019 - 12:16
Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the...
Tue, 07/02/2019 - 07:28
Abstract Nowadays, Listeria monocytogenes continues to be a major health issue. Therefore, improvements in the speed and reliability of its detection are still needed. In the present study, the combination of real‐time Recombinase Polymerase Amplification (qRPA) with immunomagnetic separation (IMS) is described. The proposed methodology was tested against a real‐time PCR method, and was...
Tue, 07/02/2019 - 05:54
Abstract Objectives This study investigates the frequency and characteristics of carbapenemase‐producing Escherichia coli/Klebsiella pneumoniae (CPE/K) and extended‐spectrum cephalosporinase‐producing E. coli/K. pneumoniae (ESCE/K) in healthy humans and livestock in rural Cambodia. Additionally, household practices as risk factors for faecal carriage of ESCE/K are identified. Methods Faecal...
Tue, 07/02/2019 - 01:02
Publication date: Available online 29 June 2019Source: LWTAuthor(s): J. Arévalo Camargo, K. Murray, K. Warriner, W. LubitzAbstractAir flow characteristics and decontamination efficacy are investigated within a forced-air ozone reactor designed to control Listeria monocytogenes on apple fruit. The commercial reactor introduced an air-ozone mix through a column of apples (540 kg contained within...
Tue, 07/02/2019 - 01:02
Available online 1 July 2019 Author(s): Marie Limoges, Catherine DonnellyAbstractThe U.S. Food and Drug Administration's (FDA) 2015 Domestic and Imported Cheese and Cheese Products Compliance Program Guidelines (CPG) (U.S. FDA, 2015) consider cheeses to be adulterated if non-toxigenic Escherichia coli levels of greater than 10 most probable number per gram (MPN/g) and less than 100 MPN/g are...
Mon, 07/01/2019 - 20:00
UK public health organisations perform routine antimicrobial susceptibility tests (ASTs) to characterise the potential for antimicrobial resistance in Salmonella enterica serovars. Genetic determinants of these r...
Mon, 07/01/2019 - 20:00
Cronobacter sakazakii is an emerging foodborne pathogen, which is linked to life-threatening infections causing septicemia, meningitis and necrotizing enterocolitis. These infections have been epidemiologically connected to ingestion of contaminated reconstituted powder infant formula. Even at low water activity C. sakazakii can survive for a long time; it is capable of protective biofilm...
Mon, 07/01/2019 - 11:32
Salmonella enterica serovar Typhimurium induces inflammatory diarrhea and bacterial uptake into intestinal epithelial cells using the Salmonella pathogenicity island 1 (SPI1) type III secretion system (T3SS). HilA activates transcription of the SPI1 structural components and effector proteins. Expression of hilA is activated by HilD, HilC, and RtsA, which act in a complex feed-forward regulatory...
Mon, 07/01/2019 - 11:32
In open environments such as water, enterohemorrhagic Escherichia coli O157:H7 responds to inorganic phosphate (Pi) starvation by inducing the Pho regulon controlled by PhoB. This activates the phosphate-specific transport (Pst) system that contains a high-affinity Pi transporter. In the pst mutant, PhoB is constitutively activated and regulates the expression of genes in the Pho regulon. Here we...
Mon, 07/01/2019 - 11:00
Detection of pathogens is crucial in food production areas. While it is well established, swabbing as a state-of-the-art sampling method offers several drawbacks with respect to yield, standardization, overall handling, and long-term monitoring. This led us to develop and evaluate a method that is easier to use at a lower cost and that should be at least as sensitive. After evaluating sundry...
Mon, 07/01/2019 - 11:00
Salmonella enterica is increasingly linked to disease outbreaks associated with consumption of low-water-activity (low-aw) foods. Persistence of the pathogen in these foods was attributed to its ability to implement desiccation resistance mechanisms. Published knowledge about methods that disrupt desiccation resistance in S. enterica is lacking. We hypothesize that strong membrane-active...
Mon, 07/01/2019 - 11:00
Salmonella spp. are among the most important foodborne pathogens and the third leading cause of human death among diarrheal diseases worldwide. Animals are the primary source of this pathogen, and animal-based foods are the main transmission route to humans. Thus, understanding the global epidemiology of Salmonella serovars is key to controlling and monitoring this bacterium. In this context,...
Mon, 07/01/2019 - 11:00
Minicircle DNA (mcDNA), which contains only the necessary components for eukaryotic expression and is thus smaller than traditional plasmids, has been designed for application in genetic manipulation. In this study, we constructed a novel plasmid containing both the Cre recombinase under the phosphoglycerate kinase (PGK) promoter and recombinant lox66 and lox71 sites located outside the...
Mon, 07/01/2019 - 11:00
Detection of salmonellae within poultry environments is an important component of many food safety programs, but sampling approaches vary greatly and may not enable the detection of salmonellae when bacteria are present at a low prevalence or concentration. Intensive longitudinal sampling within caged sheds enabled us to undertake a longitudinal analysis of the spatial distribution of salmonellae...
Mon, 07/01/2019 - 11:00
Essential oils (EOs) have demonstrated wide-spectrum antimicrobial activities and have been actively studied for their application in foods as alternative natural preservatives. However, information regarding microbial adaptive responses and changes in virulence properties following sublethal EO exposure is still scarce. The present study investigated the effect of sublethal thymol (Thy),...
Mon, 07/01/2019 - 01:08
Publication date: Available online 29 June 2019 Innovative Food Science & Emerging TechnologiesAuthor(s): Veli Gök, Simge Aktop, Mehmet Özkan, Oktay TomarAbstractIn this study the effectiveness of cold atmospheric plasma (CAP) in inactivating two bacterial pathogens on inoculated pastırma was investigated. In addition, the effect of this treatment on the total mesophilic aerobic bacteria (...
Mon, 07/01/2019 - 01:05
Available online 30 June 2019 Author(s): Linshu Lan, Ru Zhang, Xianglian Zhang, Hui ShiAbstractFoodborne pathogens can survive as the state of sublethal injury in adverse environments and recover physiological function and virulence under suitable conditions. Slightly acidic electrolyzed water (SAEW) is a non-thermal treatment to decontaminate in food industry and can induce sublethal injury....
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Debao Niu, Qian-Yi Wang, Er-Fang Ren, Xin-An Zeng, Lang-Hong Wang, Tian-Fu He, Qing-Hui Wen, Charles S. BrennanAbstractEugenol (2-Methoxy-4-(2-Propenyl) Phenol; EUG) is a natural antibacterial ingredient of the essential oil from many aromatic plants. The antimicrobial mechanism of EUG and its combined inactivation effect with pulsed electric fields (PEF)...
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Qisen Xiang, Wenjie Wang, Dianbo Zhao, Liyuan Niu, Ke Li, Yanhong BaiAbstractThis study was designed to evaluate the synergistic antimicrobial effect of plasma-activated water (PAW) combined with mild heat (40, 50, and 60 °C) against Escherichia coli O157:H7. Populations of E. coli O157:H7 were reduced from about 8.28 log CFU/mL to undetectable levels...
Mon, 07/01/2019 - 00:00
Escherichia coli O157:H7 and Salmonella choleraesuis are two important foodborne pathogens that cause illnesses and even deaths. Rapid and convenient methods, such as immunochromatographic assays (ICAs), are useful for detecting these two pathogens. Herein, we developed a highly sensitive ICA for the simultaneous quantitative analysis of these two foodborne pathogens. Silver...
Sun, 06/30/2019 - 07:43
Co‐encapsulation of lupulon and xanthohumol, as major bioactive components of hop in lecithin‐based nanoliposomes was developed by sonication method. The procedure of production was optimized by considering the duration and the power of ultrasound device as well as the concentration of lecithin as variables for the Response Surface Methodology. The average particle size, ζ‐potential, and the...
Sun, 06/30/2019 - 01:12
Available online 29 June 2019 Author(s): D. Lindsay, S. Laing, K.I. Fouhy, L. Souhoka, A.K. Beaven, T.K. Soboleva, P.K. MalakarAbstractCronobacter spp. are opportunistic pathogens that must be controlled in infant powder manufacturing plants. This study evaluated the spread of Cronobacter cells via contact surfaces within a dairy manufacturing environment. Transfer rates of Cronobacter spp. were...

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