Bacterial pathogens

Thu, 06/04/2015 - 06:20
LWT
Publication date: October 2015 Source:LWT - Food Science and Technology, Volume 63, Issue 2 Author(s): Fernanda Rodrigues Leite Rolim , Karina Maria Olbrich dos Santos , Samuel Carneiro de Barcelos , Antônio Sílvio do Egito , Thais Santana Ribeiro , Maria Lúcia da Conceição , Marciane Magnani , Maria Elieidy Gomes de Oliveira , Rita de Cássia Ramos do Egypto Queiroga This study...
Thu, 06/04/2015 - 06:20
LWT
Publication date: October 2015 Source:LWT - Food Science and Technology, Volume 63, Issue 2 Author(s): Rekha Priyadarshini Albert Dhayakaran , Suresh Neethirajan , Jun Xue , John Shi Pathogenic biofilms that form on food processing equipment/surfaces are of great concern, because these can readily lead to food spoilage, bio-fouling, food-borne illness, and their recalcitrance can result...
Thu, 06/04/2015 - 06:20
LWT
Publication date: October 2015 Source:LWT - Food Science and Technology, Volume 63, Issue 2 Author(s): Ghada M. Khiralla , Bahig A. El-Deeb Microbial biofilms, which characterized by their resistance to the traditional antimicrobials, are considered as a renewable source of contamination by pathogens. As alternative antimicrobial and antibiofilm agent selenium nanoparticles (SeNPs) were...
Thu, 06/04/2015 - 01:33
Publication date: 9 July 2015 , Volume 178, Issues 1–2 Author(s): Migma Dorji Tamang , Mamata Gurung , Hyang-Mi Nam , Dong Chan Moon , Su-Ran Kim , Geum-Chan Jang , Dae-Yeong Jung , Suk-Chan Jung , Yong-Ho Park , Suk-Kyung Lim This study compared the prevalence of Salmonella spp. and their antimicrobial susceptibilities in pigs from conventional and organic farms during 2012–2013 in...
Thu, 06/04/2015 - 01:33
This study aimed at using essential oilEO) alone or combined EO with nisin and γ-irradiation to control Salmonella Typhimurium during the refrigerated storage of mini-carrots. Peeled mini-carrots were inoculated with S. Typhimurium at a final concentration of approximately 7 log CFU/g. Inoculated samples were coated by 5 different coating solutions: (i) nisin solution at final concentration of...
Thu, 06/04/2015 - 01:33
Aims The effect of milk fat content on ohmic heating compared to conventional heating for inactivation of food-borne pathogens was investigated. Methods and Results Sterile cream was mixed with sterile buffered peptone water and adjusted to 0, 3, 7, 10% (w/v) milk fat content. These samples with varying fat content were subjected to ohmic and conventional heating. The effect of milk fat on...
Wed, 06/03/2015 - 01:20
Publication date: 2 September 2015 , Volume 208 Author(s): Yaoxin Huang , Robert Sido , Runze Huang , Haiqiang Chen We developed and evaluated a small scaled-up water-assisted pulsed light (WPL) system, in which berries were washed in a flume washer while being irradiated by pulsed light (PL). Hydrogen peroxide (H 2 O 2 ) was used in combination with PL as an advanced oxidation process...
Wed, 06/03/2015 - 01:20
The growth behavior of Listeria monocytogenes in the presence of typical spoilage microorganisms on poultry breast fillets (Pseudomonas spp., Brochothrix thermosphacta, Enterobacteriaceae, Lactobacillus spp., total viable count) was investigated. The aim of the study was to compare the growth of L. monocytogenes under oxygen- and nitrogen-enriched atmospheres and to figure out possible...
Tue, 06/02/2015 - 01:29
Bhagawat S. Chandrasekar, Shikha Yadav, Emmanuel S. Victor, Shamik Majumdar, Mukta Deobagkar-Lele, Nitin Wadhwa, Santosh Podder, Mrinmoy Das, Dipankar Nandi Interferon-gamma (Ifnγ), a key macrophage activating cytokine, plays pleiotropic roles in host immunity. In this study, the ability of Ifnγ to induce the aggregation of resident mouse adherent peritoneal exudate cells (APECs), consisting...
Tue, 06/02/2015 - 01:29
In this work, the combined effect of bioactive compounds (tea tree essential oil, propolis extract and gallic acid) and storage temperature (15 and 5C) on microbiological and sensory quality of fresh-cut celery, leek and butternut squash was studied. It was confirmed that a good control of refrigeration temperature limits the growth of spoilage and pathogenic microorganisms. Tea tree essential...
Mon, 06/01/2015 - 01:19
Publication date: October 2015 , Volume 51 Author(s): Hanna Lee , Jung Eun Kim , Myong-Soo Chung , Sea C. Min Microwave-powered cold plasma treatment (CPT) was evaluated as a means to improve the microbiological safety of fresh vegetables and dried fruits. The CPT at 900 W, conducted for 10 min using nitrogen as a plasma-forming gas, inactivated Salmonella Typhimurium inoculated on...
Mon, 06/01/2015 - 01:19
Publication date: October 2015 , Volume 51 Author(s): Edward M. Fox , Patrick G. Wall , Séamus Fanning Surveillance and control of food-borne human pathogens, such as Listeria monocytogenes , is a critical aspect of modern food safety programs at food production facilities. This study evaluated contamination patterns of Listeria species at a poultry food production facility, and evaluated...
Sun, 05/31/2015 - 20:00
Abstract FTIR spectra of poultry meat specific bacteria viz. Salmonella enteritidis, Pseudomonas ludensis, Listeria monocytogenes and Escherichia coli were collected and investigated for identification of spectral windows capable of bacterial classification and quantification. Two separate datasets obtained at different times were used in the study to check reproducibility of...
Sun, 05/31/2015 - 01:23
This study investigated the impact of composting and lagoon storage on survival and change in diversity of tetracycline-resistant (Tcr) and erythromycin-resistant (Emr) bacteria and the resistance genes they carry in swine manure. Treatments were arranged as a 2 x 2 factorial design: composting vs. lagoon storage and 0 vs. 1% Surround™ WP Crop Protectant (a clay product) in three replicates....
Sun, 05/31/2015 - 01:23
Enterohemorrhagic Escherichia coli O157 (O157) strains can be classified in clades by single nucleotide polymorphisms (SNPs), but this analysis requires significant laboratory effort. Since the distribution of insertion sequence (IS) 629 insertions has been reported to be biased among different clades, O157 isolates can be putatively classified in clades by comparison with an IS629 distribution...
Sun, 05/31/2015 - 01:23
Vibrio parahaemolyticus (Vp) and Vibrio vulnificus (Vv) are associated with the consumption of raw oysters and cause illnesses ranging from simple gastroenteritis to life-threatening septicemia. These halophilic bacteria are frequently found in marine and estuarine systems, accumulating within the tissues of a number of aquatic organisms and passing on to humans after consumption, through...
Sun, 05/31/2015 - 01:23
Aims We investigated the effect of ozone treatment of apple juice at different pH levels for inactivation of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes. Methods and Results Apple juice (pH 3.0, 4.0 and 5.0) inoculated with the three pathogens were treated with gaseous ozone (3.0 l min−1 flow rate and 2.0-3.0 g m−3) for up to 4 min. Ozone treatment (4 min) of pH...
Sun, 05/31/2015 - 01:23
Aims In the present work the Libyan wild-growing Thymus capitatus essential oil was evaluated for its biological properties. Methods and Results Carvacrol (68.19%) and thymol (12.29%) were found to be the main compounds of the oil. Antioxidant properties, determined by 2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were 119 μg ml-1, 403 μg ml-1and 105 μg ml-1 for oil, thymol...
Sat, 05/30/2015 - 01:22
Ready-to-eat luncheon meats sliced in retail delis have been found to pose the greatest risk of foodborne illness from Listeria monocytogenes among all ready-to-eat foodsSlicers used in delis have many removable parts that are connected with seals and gaskets, with spaces, cracks and crevices that are difficult to clean adequately and may provide a niche for L. monocytogenes survival. Standard...
Fri, 05/29/2015 - 06:20
Publication date: Available online 28 May 2015 Author(s): Bradley L. Bearson , Brian W. Brunelle Fluoroquinolones are broad-spectrum antibiotics that inhibit bacterial DNA gyrase and topoisomerase activity, which can cause DNA damage and result in bacterial cell death. In response to DNA damage, bacteria induce an SOS response to stimulate DNA repair. However, the SOS response may also...
Thu, 05/28/2015 - 06:20
In this study, the antibiofilm activity of citric acid treatment on Staphylococcus aureus strains isolated from raw milk samples was evaluated. For this purpose, the prevention and removal of biofilm formation of S. aureus strains by citric acid treatments (2% and 10%) for 20 min were investigated for comparison with peracetic acid treatment (0.3%) on both microtitration plate and stainless steel...
Thu, 05/28/2015 - 01:27
A new monophasic variant of Salmonella Typhimurium, Salmonella enterica serotype 4,12:i:-, is rapidly emerging. This serotype is now considered to be among the 10 most common serovars isolated from humans in many countries in Europe and in the United States. The public health risk posed by these emerging monophasic Salmonella Typhimurium strains is considered comparable to that of classical...
Thu, 05/28/2015 - 01:27
Fowl typhoid and pullorum disease, caused by Salmonella enterica serovars Gallinarum biovars Salmonella Gallinarum (S. Gallinarum) and Salmonella Pullorum (S. Pullorum), remain large threat to the organic poultry industry. These infections are serious threats to poultry health and overall flock viability especially at their early age. These avian pathogens cause a significant production loss and...
Thu, 05/28/2015 - 01:27
Staphylococcus aureus is an important foodborne pathogen on global basisThe current study investigated the genetic patterns in S. aureus isolates from leaf vegetables (n = 53). Additional isolates from livestock (n = 31) and humans (n = 27) were compared with the leaf vegetable isolates. Genes associated with toxins, antibiotic resistance, and pulsed-field gel electrophoresis (PFGE) patterns were...
Thu, 05/28/2015 - 01:27
Clostridium perfringens produces diverse virulent toxins that cause necrotic enteritis in poultry, resulting in a great negative impact on the poultry industry. To study the characteristics of C. perfringens in chickens, we isolated 88 strains from chickens (1 strain per flock) with necrotic enteritis. The isolated bacterial strains were screened for toxin type and antimicrobial susceptibility....
Thu, 05/28/2015 - 01:27
An observational study examining prevalence of Campylobacter and Salmonella in environmental samples and biosecurity interventions to mitigate these 2 pathogens was performed for 3 consecutive flocks on a commercial broiler farm in northeast Georgia. Monitoring was performed first on a baseline flock for which no interventions or treatments were undertaken. The same monitoring was performed for 2...
Thu, 05/28/2015 - 01:27
In-house litter composting has been reintroduced to the industry and shown to reduce bacteria by as much as 2 orders of magnitude. Other industries have demonstrated that pathogens can recolonize a waste-residual when microbial competition has been reduced or inhibited following composting. Poultry growers, in the process of shifting to in-house composting for pathogen control, should be aware of...
Thu, 05/28/2015 - 01:27
Campylobacter is a natural member of the gut microflora in many commercial broilers and as such can become a contaminant on edible surfaces during processing. Culturing gut contents or feces can be a means to determine flock status prior to live-haul. The wide variety of non-Campylobacter background bacteria in these complex samples contaminates growth media and can make it very difficult to...
Thu, 05/28/2015 - 01:27
This review presents a framework for assessing the efficacy of antimicrobials used to control necrotic enteritis (NE) caused by Clostridium perfringens (CP) in the context of susceptibility testing and clinical efficacy, and their potential interactions with the intestinal microbiota of poultry. Practitioners have traditionally based their choice of antimicrobial agent on antimicrobial...
Wed, 05/27/2015 - 06:20
e-First Articles. This study aimed to isolate and characterize bacteriophages that lyse non-O157 Shiga toxin-producing Escherichia coli (STEC) from cattle feces. Of 37 non-O157 STEC-infecting phages isolated, those targeting O26 (AXO26A, AYO26A, AYO26B), O103 (AXO103A, AYO103A), O111 (AXO111A, AYO111A), O121 (AXO121A, AXO121B), and O145 (AYO145A, AYO145B) were further characterized. Transmission...
Wed, 05/27/2015 - 01:19
Publication date: October 2015 , Volume 51 Author(s): H. Liu , Y.D. Niu , R. Meng , J. Wang , J. Li , R.P. Johnson , T.A. McAllister , K. Stanford Efficacy of four bacteriophages (phages) and a cocktail for biocontrol of Escherichia coli O157 was assessed on beef samples stored at 4, 22 and 37 °C. Samples (3 × 3 × 1 cm) were contaminated with E. coli O157 (104 CFU/cm2) and treated...
Tue, 05/26/2015 - 03:20
In the present paperthe chemical composition of Helichrysum italicum oil was analyzed by gas chromatography–mass spectrometry (GC-MS). Neryl acetate (32.65%) was the main component. The minimum inhibitory concentration and minimum bactericidal concentration of H. italicum oil against several bacteria were evaluated. The results showed that H. italicum oil exhibited high antibacterial activity...
Mon, 05/25/2015 - 20:00
Abstract Chemically modified biofunctional chitosan derivatives may open up new applications in functional food and nutraceuticals development. Two new conjugates, namely vanillic acid and coumaric acid grafted chitosan derivatives were developed and comparative evaluation of their antioxidant and antimicrobial activities was carried out with ferulic acid and gallic acid grafted...
Mon, 05/25/2015 - 01:17
Publication date: 2 September 2015 , Volume 208 Author(s): D.C. Aryani , H.M.W. den Besten , W.C. Hazeleger , M.H. Zwietering Prediction of microbial growth kinetics can differ from the actual behavior of the target microorganisms. In the present study, the impact of strain variability on maximum specific growth rate ( μ max ) (h− 1) was quantified using twenty Listeria monocytogenes...
Sun, 05/24/2015 - 01:22
Publication date: 2 September 2015 , Volume 208 Author(s): Stefanie Delbeke , Siele Ceuppens , Liesbeth Jacxsens , Mieke Uyttendaele Enteric pathogens, such as Salmonella spp. and pathogenic Escherichia coli , have been detected and associated with food borne outbreaks from (imported) fresh leafy herbs. Screening on imported herbs from South East Asian countries has been described....
Sat, 05/23/2015 - 06:20
Publication date: Available online 4 May 2015 Author(s): Valérie Thomas , Anno de Jong , Hilde Moyaert , Shabbir Simjee , Farid El Garch , Ian Morrissey , Hervé Marion , Michel Vallé VetPath is an ongoing pan-European antimicrobial susceptibility monitoring programme collecting pathogens from diseased cattle, pigs and poultry not recently treated with antibiotics. Non-replicate milk...
Sat, 05/23/2015 - 03:20
Salmonellae survive and propagate in macrophages to cause serious systemic disease. Periplasmic superoxide dismutase plays a critical role in this survival by combating phagocytic superoxide. Salmonella Typhimurium strain 14028 produces two periplasmic superoxide dismutases, SodCI and SodCII. Although both proteins are produced during infection, only SodCI is functional in the macrophage...
Sat, 05/23/2015 - 03:20
Vibrio cholerae uses a multiprotein transcriptional regulatory cascade to control expression of virulence factors cholera toxin and toxin-coregulated pilus. Two proteins in this cascade are ToxR and TcpP – unusual membrane-localized transcription factors with relatively undefined periplasmic domains and transcription activator cytoplasmic domains. TcpP and ToxR function with each other and two...
Sat, 05/23/2015 - 03:20
Campylobacter concisus is an oral bacterium that has been shown to be associated with inflammatory bowel disease (IBD). In this study we examined clusters of oral C. concisus strains isolated from patients with IBD and healthy controls by analysing the six housekeeping genes that we previously described. In addition, we investigated the population structure of C. concisus strains. Whether oral...
Sat, 05/23/2015 - 01:21
Publication date: 3 August 2015 , Volume 206 Author(s): Joshua B. Gurtler , Arthur Hinton Jr. , Rebecca B. Bailey , William C. Cray Jr. , Richard J. Meinersmann , Takiyah A. Ball , Tony Z. Jin The Egg Products Inspection Act of 1970 requires that egg products in the U.S. must be pasteurized prior to release into commerce. The USDA Food Safety and Inspection Service (FSIS) is...
Sat, 05/23/2015 - 01:21
Filipe Carvalho, Magda L. Atilano, Rita Pombinho, Gonçalo Covas, Richard L. Gallo, Sérgio R. Filipe, Sandra Sousa, Didier Cabanes Listeria monocytogenes is an opportunistic Gram-positive bacterial pathogen responsible for listeriosis, a human foodborne disease. Its cell wall is densely decorated with wall teichoic acids (WTAs), a class of anionic glycopolymers that play key roles in bacterial...
Sat, 05/23/2015 - 01:21
Andrew T. Cheng, Karen M. Ottemann, Fitnat H. Yildiz Two-component signal transduction systems (TCS) are used by bacteria to sense and respond to their environment. TCS are typically composed of a sensor histidine kinase (HK) and a response regulator (RR). The Vibrio cholerae genome encodes 52 RR, but the role of these RRs in V. cholerae pathogenesis is largely unknown. To identify RRs that...
Sat, 05/23/2015 - 01:21
Publication date: 3 August 2015 , Volume 206 Author(s): Evy Lahou , Xiang Wang , Elien De Boeck , Elien Verguldt , Annemie Geeraerd , Frank Devlieghere , Mieke Uyttendaele In order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (D-value) of three strains of...
Fri, 05/22/2015 - 01:19
Ultraviolet lightUV) has antimicrobial effects, but the shadowing effect has limited its application. In this study, a novel setup using UV processing in agitated water was developed to inactivate Escherichia coli O157:H7 and Salmonella on blueberries. Blueberries were dip- or spot-inoculated with E. coli or Salmonella. Blueberries inoculated with E. coli were treated for 2 to 10 min with UV...
Fri, 05/22/2015 - 01:19
Accumulating evidence suggests that bacteriocin production represents a probiotic trait for intestinal strains to promote dominance, fight infection, and even signal the immune system. In this respect, in a previous study, we isolated from the porcine intestine a strain of Streptococcus hyointestinalis DPC6484 that displays antimicrobial activity against a wide range of Gram-positive bacteria and...

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