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Bacterial pathogens

Mon, 04/22/2019 - 17:00
Janina Noster, Tzu-Chiao Chao, Nathalie Sander, Marc Schulte, Tatjana Reuter, Nicole Hansmeier, Michael Hensel Intracellular Salmonella enterica serovar Typhimurium (STM) deploy the Salmonella Pathogenicity Island 2-encoded type III secretion system (SPI2-T3SS) for the massive remodeling of the endosomal system for host cells. This activity results in formation of an extensive interconnected...
Mon, 04/22/2019 - 11:14
The bacterial flagellum is a sophisticated self-assembling nanomachine responsible for motility in many bacterial pathogens including Pseudomonas aeruginosa, Vibrio spp., and Salmonella enterica. The bacteria flagellum has been studied extensively in the model systems Escherichia coli and Salmonella Typhimurium, yet the range of variation in flagellar structure and assembly remains incompletely...
Mon, 04/22/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 5, Page 834-843, May 2019.
Mon, 04/22/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 5, Page 834-843, May 2019.
Mon, 04/22/2019 - 01:13
Available online 19 April 2019 Author(s): S. Sivakumar, T. Raja Swaminathan, R. Anandan, N. KalaimaniAbstractThe lack of shrimp cell lines and difficulty in establishing shrimp cell culture systems, with an appropriate medium is a major concern in the aquaculture sector. The present study attempts to address this issue by developing an in vitro cell culture system from various tissues (hemocytes...
Mon, 04/22/2019 - 01:13
Available online 20 April 2019 Author(s): Xueran Mei, Xiwen Zhai, Changwei Lei, Xiaolan Ye, Zhuangzhuang Kang, Xuan Wu, Rong Xiang, Yulong Wang, Hongning WangAbstractSalmonella strains are major foodborne pathogens for the animals and humans, presenting a significant threat to food safety and public health worldwide. The rapid and accurate diagnosis of Salmonella infection is required for...
Mon, 04/22/2019 - 01:13
Available online 20 April 2019 Author(s): Ahmed Abdel-Hameid Ahmed, Nagah Mohammed Saad Maharik, Antonio Valero, Sahar Mahmoud KamalAbstractStaphylococcus aureus (S. aureus) is one of the most concerning foodborne pathogens due to its ability to produce wide varieties of heat-stable enterotoxins. Hence, this study was designed to investigate the prevalence of the enterotoxigenic S. aureus strains...
Mon, 04/22/2019 - 00:00
Abstract Miang is a traditional fermented tea made from fermentation of Assam tea leaves with mixed microbial culture involving lactic acid bacteria and yeast. Miang has important bioactive benefits such as antioxidant and antimicrobial activity with relevance to health benefits. Miang is categorized into two processes; filamentous fungi growth-based (FFP) and non-filamentous...
Mon, 04/22/2019 - 00:00
Toxins, Vol. 11, Pages 233: Structural Insights to the Heterotetrameric Interaction between the Vibrio parahaemolyticus PirAvp and PirBvp Toxins and Activation of the Cry-Like Pore-Forming Domain Toxins doi: 10.3390/toxins11040233 Authors: Shin-Jen Lin Yi-Fan Chen Kai-Cheng Hsu Yun-Ling Chen Tzu-Ping Ko Chu-Fang Lo Han-Ching Wang Hao-Ching Wang Acute hepatopancreatic...
Mon, 04/22/2019 - 00:00
Toxins, Vol. 11, Pages 232: Clostridium perfringens Delta-Toxin Damages the Mouse Small Intestine Toxins doi: 10.3390/toxins11040232 Authors: Soshi Seike Masaya Takehara Keiko Kobayashi Masahiro Nagahama Clostridium perfringens strains B and C cause fatal intestinal diseases in animals. The secreted pore-forming toxin delta-toxin is one of the virulence factors of the strains, but...
Sun, 04/21/2019 - 01:12
Available online 19 April 2019 Author(s): Yufei Wang, Yuehua Ke, Cuijuan Duan, Xueping Ma, Qinfang Hao, Lijie Song, Xiaojin Guo, Tao Sun, Wei Zhang, Jing Zhang, Yiwen Zhao, Zhijun Zhong, Xiaoli Yang, Zeliang ChenAbstractBrucella species are the causative agents of brucellosis, a worldwide zoonotic disease that affects a broad range of mammals and causes great economic losses. Small regulatory...
Sat, 04/20/2019 - 09:33
Abstract Salmonella enterica serovar Typhimurium (S. Typhimurium) inhabits a wide range of hosts, including poultry, and causes acute gastroenteritis in humans that may result in death. Superoxide dismutase (SOD) is an important antioxidant enzyme present in nearly all living cells exposed to oxygen. Recently, we reported the novel roles of SOD in serum resistance and biofilm formation in S....
Sat, 04/20/2019 - 00:00
Abstract Sanguisorba officinalis L., one kind of perennial plants, has been widely distributed in southern Europe, northern Africa and China. The objective of the present work was to evaluate the antibacterial captivities of polyphenolic extract (PE) of S. officinalis L. on five pathogenic bacteria, including Gram-negative bacteria (Escherichia coli and Salmonella typhimurium)...
Fri, 04/19/2019 - 11:19
Abstract Aims The aim of this study was to develop a multiplex touchdown PCR (multiplex TD‐PCR) for rapid and simultaneous detection of four major foodborne pathogens to avoid mispriming and unwanted production during gene amplification. Touchdown PCR is the modified form of standard PCR, which enhances specificity, sensitivity. Methods and Results For this reason, a multiplex TD‐PCR assay...
Thu, 04/18/2019 - 10:31
Pomegranate peel is rich in polyphenols which are known to have antibacterial and antifungal properties. Summary Pomegranate peel (PoP) is a byproduct of the fruit juice processing industry, comprising nearly 30–40% of fruit portion. PoP is rich in polyphenols (phenolic acids, tannins and flavonoids particularly anthocyanins) which are known to have diverse biological functions including...
Thu, 04/18/2019 - 10:31
The effect of pressure, extraction time and ethanol concentration on some bioactive compounds extraction from yellow prickly pear peels was measured. The ethanol concentration showed the highest impact, followed by high pressure and then by the extraction time. The models developed by RSM showed high correlations, good fitting and adequacy to describe the experimental data. Summary The main...
Thu, 04/18/2019 - 10:31
Application of orange lipases for plant oil biotransformation to increase biological activities. Summary The aim of this study was to show that abundant and inexpensive plant oils can be biotransformed to increase biological activity (antioxidant, antimicrobial and cytotoxicity) through hydrolysis reaction catalysed by lipases. We tested homemade and commercial lipases through the...
Thu, 04/18/2019 - 10:31
Graphical abstract of low‐salt solid‐state fermentation of Yunnan oil furu. Summary The effect of reducing salt on the microflora, enzymatic activity and structural, textural and sensory properties of Yunnan oil furu was studied. Oil furu with different salt content, including 11 (control), 9%, 7% and 5%, were prepared. The results showed that lower salt increased fungus and lactic acid...
Thu, 04/18/2019 - 10:05
Listeria monocytogenes is one of the most common foodborne pathogens. Poultry meat and products are of the main vehicles of pathogenic strains of L. monocytogenes for human. Poultry products are part of the regular diet of people and, due to nutrient content, more content of protein, and less content of fat, gain more attention. In comparison with red meat, poultry meat is more economical. So, it...
Thu, 04/18/2019 - 01:13
Available online 16 April 2019 Author(s): Wenxin Liu, Chaowen Yuan, Liguo Zhang, Yufei FengAbstractThe objective of this study was to establish a novel isothermal amplification method for detection of heat-labile enterotoxin (LT-I)-producing Escherichia coli. Loop-mediated isothermal amplification (LAMP), cross-priming amplification (CPA), and isothermal multiple-self-matching-initiated...
Thu, 04/18/2019 - 01:13
Publication date: Available online 16 April 2019 Innovative Food Science & Emerging TechnologiesAuthor(s): Clarissa Detomi de Albuquerque, Sébastien Curet, Lionel BoillereauxAbstractThis study coupled a 3D-CFD and heat transfer finite elements model with the microbial inactivation approach proposed by Geeraerd, Herremans, and Van Impe (2000). The CFD-heat transfer model was developed using...
Wed, 04/17/2019 - 20:00
Antimicrobial Resistance, Virulence Determinants, and Biofilm Formation of Enterococcus Species From Ready-to-Eat Seafood Etinosa O. Igbinosa1,2* and Abeni Beshiru1 1Applied Microbial Processes and Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria 2Sustainable Development Office, University of Benin, Benin City,...
Wed, 04/17/2019 - 20:00
The serious human pathogen, E. coli serotype O157:H7, continues to gain antibiotic resistance, posing a public health threat. While this serotype’s genome has been sequenced, the role of 25% of its genes remains ...
Wed, 04/17/2019 - 20:00
Salmonella enterica Growth Conditions Influence Lettuce Leaf Internalization Yulia Kroupitski1, Rachel Gollop1, Eduard Belausov2, Riky Pinto1 and Shlomo Sela (Saldinger)1* 1Microbial Food-Safety Research Unit, Department of Food Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel 2Confocal Microscopy Unit, Institute of Plant Sciences, Agricultural Research...
Wed, 04/17/2019 - 09:15
Abstract Commercial fermentation for bulk preservation of cucumbers relies on natural microbiota and approximately 1 M sodium chloride (NaCl) brines, resulting in large volumes of high‐salt wastewater. An alternative process utilizing 0.1 M calcium chloride (CaCl2) as the only salt was developed to eliminate NaCl from fermentation brines for reduced environmental impact. This study determined the...
Wed, 04/17/2019 - 00:00
Coxiella burnetii is a gram-negative and polymorphic rod bacterium that causes Q fever, a common zoonotic disease distributed worldwide. Widespread occurrences of the disease outbreaks indicate the importance of coordinated animal and human health efforts to control these outbreaks. Different tests are available to determine the C. burnetii infection status of a flock, but false negative...
Tue, 04/16/2019 - 20:00
Large-Scale Genomic Analyses and Toxinotyping of Clostridium perfringens Implicated in Foodborne Outbreaks in France Abakabir Mahamat Abdelrahim, Nicolas Radomski, Sabine Delannoy, Sofia Djellal, Marylène Le Négrate, Katia Hadjab, Patrick Fach, Jacques-Antoine Hennekinne, Michel-Yves Mistou and Olivier Firmesse* Université PARIS-EST, Agence Nationale de Sécurité...
Tue, 04/16/2019 - 20:00
Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae Susanne Brenzinger1*, Lizah T. van der Aart1, Gilles P. van Wezel1, Jean-Marie Lacroix2, Timo Glatter3 and Ariane Briegel1* 1Department of Microbial Biotechnology & Health, Institute of Biology Leiden, Leiden University, Leiden, Netherlands 2Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576...
Tue, 04/16/2019 - 12:44
Abstract Thyme oil nanoemulsions (TONa and TONb), having 219 nm and 163 nm diameters, were successfully prepared by using sonication technique. Zeta potential (ZP) and polydispersity indexes (PDI) of TONa and TONb were defined to be 19.77, 0.24 and −24.80, 0.054, respectively. It was determined that zeta size values of TONs might have played a role on the inhibition of Pseudomonas aeruginosa,...
Tue, 04/16/2019 - 12:44
Abstract Thyme oil nanoemulsions (TONa and TONb), having 219 nm and 163 nm diameters, were successfully prepared by using sonication technique. Zeta potential (ZP) and polydispersity indexes (PDI) of TONa and TONb were defined to be 19.77, 0.24 and −24.80, 0.054, respectively. It was determined that zeta size values of TONs might have played a role on the inhibition of Pseudomonas aeruginosa,...
Tue, 04/16/2019 - 06:51
Abstract Aims The aim of this study was the molecular identification of Salmonella enterica serovar Infantis isolated from poultry samples and their antimicrobial resistance and virulence profiling. Methods and Results A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected poultry post mortem specimens reported by us earlier were preliminary identified...
Tue, 04/16/2019 - 01:13
Publication date: Available online 14 April 2019Source: Author(s): Sharif Najafi, Ehsan Sanati, Mahsa Khademi, Fateme Abdorrazaghi, Reza Kazempoor Mofrad, Zahra RezasoltaniAbstractIn recent years, there is a growing interest in new medical applications of botulinum toxin, including pain control, osteoarthritis treatment, and wound healing. While clinical applications of botulinum toxin seem...
Tue, 04/16/2019 - 01:10
Available online 13 April 2019 Author(s): Minerva Rivera-Reyes, Jonathan A. Campbell, Catherine N. CutterAbstractShiga toxin-producing Escherichia coli (STEC) are known to adapt and survive acidic conditions. Acid-adaptation also may result in cross-protection against other stressors present in foods, such as reduced water activity (aw), refrigeration conditions, or the presence of antimicrobials...
Mon, 04/15/2019 - 15:00
Abstract The role of free‐ranging wildlife in the epidemiology of enteropathogens causing clinical illness in humans and domestic animals is unclear. Salmonella enterica and anti‐microbial resistant bacteria have been detected in the faeces of raccoons (Procyon lotor), but little is known about the carriage of these bacteria in other sympatric meso‐mammals. Our objectives were to: (a) report the...
Mon, 04/15/2019 - 11:36
Bacterial lipoproteins are globular proteins anchored to the extracytoplasmic surfaces of cell membranes through lipidation at a conserved N-terminal cysteine. Lipoproteins contribute to an array of important cellular functions for bacteria, as well as being a focal point for innate immune system recognition through binding to Toll-like receptor 2 (TLR2) heterodimer complexes. Although...
Mon, 04/15/2019 - 11:25
This study investigated genetic relatedness among Staphylococcus aureus from livestock, their carcass’ surfaces after slaughter, personnel, and equipment of a Greek abattoir. Genotyping was performed using PFGE, MLST, spa, and SCCmec. Antimicrobial susceptibility was determined according to EUCAST guidelines. Genes encoding staphylococcal enterotoxins (SEs) and Panton‐Valentine leukocidin (PVL)...
Mon, 04/15/2019 - 08:53
Abstract Aims This study aimed to gather information on farming practices employed in organic lettuce fields in Sao Paulo, Brazil and associate these practices with the microbiological characteristics of the products. Methods and Results Practices were surveyed using a questionnaire applied in ten farms, where 200 heads of lettuce were collected and submitted to enumeration of total coliforms...
Mon, 04/15/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 5, Page 775-784, May 2019.
Mon, 04/15/2019 - 00:00
Toxins, Vol. 11, Pages 225: Application of an Endothelial Cell Culture Assay for the Detection of Neutralizing Anti-Clostridium Perfringens Beta-Toxin Antibodies in a Porcine Vaccination Trial Toxins doi: 10.3390/toxins11040225 Authors: Olivia K. Richard Sven Springer Jacqueline Finzel Tobias Theuß Marianne Wyder Beatriz Vidondo Horst Posthaus Background: Beta-toxin (CPB) is...

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