Bacterial pathogens

Sat, 01/09/2021 - 06:18
Author(s): Chao Liao, Luxin Wang The native bacterial populations present in three brands of Spring Mix salads (brands A, B, and C) were analyzed by plating and 16S rRNA gene sequencing during 15 days of storage at 4°C. While the traditional plating method did not show significant differences among three brands or between before and after storage, 16S rRNA gene sequencing showed that the “brand”...
Sat, 01/09/2021 - 00:00
Since its initial approval in 1989 by the US Food and Drug Administration for the treatment of blepharospasm and other facial spasms, botulinum toxin (BoNT) has evolved into a therapeutic modality for a variety of neurological and non-neurological disorders. With respect to neurologic movement disorders, BoNT has been reported to be effective for the treatment of dystonia, bruxism, tremors, tics...
Sat, 01/09/2021 - 00:00
Clostridium perfringens is a serious threat to successful bovine farming. It causes severe damage to the buffalo and cattle health causing a drastic reduction in milk and meat production. In Pakistan, C. perfringens is a constant threat, and for its management, antibiotics are mostly used. Most bovine farmers use a single antibiotic to suppress the bacterial infection which in turn, increases the...
Fri, 01/08/2021 - 22:11
Background The global epidemiology and resistomes dynamics of multidrug‐resistant Citrobacter spp., Enterobacter hormaechei, Klebsiella variicola, Morganella morganii, Proteus mirabilis and Providencia spp. have not been described, despite their importance as emerging opportunistic clinical pathogens. Methods The genomes of the above‐mentioned organisms were curated from PATRIC and NCBI and used...
Fri, 01/08/2021 - 22:10
Aim To investigate the occurrence of ESBL and colistin‐resistant E. coli and its genotypic characterization and identification of virulence determinants in cases of bovine mastitis in three provinces of China. Materials & methods Five hundred and thirty‐five milk samples presenting mastitis symptoms were screened for the presence of ESBL, colistin‐resistant, and different virulence genes....
Fri, 01/08/2021 - 16:00
The rapid emergence of multidrug-resistant (MDR) bacteria is a global health problem. Mobile genetic elements like conjugative plasmids, transposons, and integrons are the major players in spreading resistance genes in uropathogenic Escherichia coli (UPEC) pathotype. The E. coli BH100 strain was isolated from the urinary tract of a Brazilian woman in 1974. This strain presents two plasmids...
Fri, 01/08/2021 - 16:00
The açai palm (Euterpe oleracea) is native to the Amazon basin, a humid tropical forest. High levels of total mesophilic bacteria with high diversity have been consistently reported in açai fruits. As local consumers have few digestive problems, the results of the present study reveal the lactic acid bacteria (LAB) recovered from açai fruits with characteristics that suggest they are possible...
Fri, 01/08/2021 - 16:00
Methicillin-resistant Staphylococcus aureus (MRSA) have been found on German dairy farms and may be the cause of difficult-to-treat bovine mastitis. Considering the one health approach, MRSA might be transmitted from animals to humans raising the risk for severe infections. On 17 German dairy farms with a history of MRSA detection, MRSA strains were isolated from quarter milk, bulk tank milk, and...
Fri, 01/08/2021 - 16:00
Dry fermented sausage is popular among the world because of its rich nutrition and unique flavor. Starter cultures play an important role in the quality of dry fermented sausage. In this study, probiotics lactic acid bacteria Lactobacillus delbrueckii N102, Latilactobacillus sakei H1-5, Debaryomyces hansenii Y4-1, and Wickerhamomyces anomalus Y12-3 were isolated from food-borne materials. The...
Fri, 01/08/2021 - 16:00
Campylobacter jejuni (CJ) is the most prevalent zoonotic pathogen of chicken meat and related products, which may lead to gastroenteritis and autoimmune diseases in humans. Although controlling this bacterium is important, CJ strains resistance against traditional antibiotic therapy has been increased. Vegetable oils and fats are natural biomaterials explored since the Ancient times, due to their...
Fri, 01/08/2021 - 16:00
Campylobacter jejuni is the leading cause of bacterial gastroenteritis, which has motivated the monitoring of genetic profiles circulating in Luxembourg since 13 years. From our integrated surveillance using a genotyping strategy based on an extended MLST scheme including gyrA and porA markers, an unexpected endemic pattern was discovered in the temporal distribution of genotypes. We aimed to...
Fri, 01/08/2021 - 16:00
In Brucella spp., the type IV secretion system (T4SS) is essential for bacterial intracellular survival and inhibition of the host innate immune response. The Brucella T4SS secretes 15 different effectors to escape host immunity and promote intracellular replication. Among them, BspF has a GNAT-family acetyltransferase domain, implying its acetyltransferase activity. We confirmed that BspF has...
Fri, 01/08/2021 - 08:02
by Caroline Robé, Katrin Daehre, Roswitha Merle, Anika Friese, Sebastian Guenther, Uwe Roesler The colonization of broilers with extended-spectrum β-lactamase- (ESBL-) and plasmid-mediated AmpC β-lactamase- (pAmpC-) producing Enterobacteriaceae has been extensively studied. However, only limited data on intervention strategies to reduce the colonization throughout the fattening period are...
Fri, 01/08/2021 - 06:10
Author(s): Ju Liu, Guoyang Xie, Qin Xiong, Taobo Liang, Hengyi Xu Cronobacter spp. are important opportunistic foodborne pathogens in powdered infant formula (PIF). In this study, a sensitive and specific assay was developed for fluorescent and colorimetric detection of Cronobacter spp. in PIF samples by using ligase chain reaction and rolling circle amplification (LCR-RCA) dual-amplification...
Fri, 01/08/2021 - 06:07
Author(s): Zhang Jian, Wei Luo, Guifeng Liu, Yang Wang, Shizhong Geng, Zhiming Pan, Xin’an Jiao Salmonella Enteritidis (SE) is important zoonotic pathogens, it can be easily transferred to human by contaminated animal products. It is necessary for epidemic surveys of SE in current modern large-scale chicken farms. And so in this study, Salmonella strains were isolated from suspected samples being...
Fri, 01/08/2021 - 04:02
The foodborne pathogen Listeria monocytogenes lives as a saprophyte in nature and can adhere to and grows on surfaces as diverse as leaves, sediment, and stainless steel. To discern the mechanisms used by L. monocytogenes for attachment and growth on various surfaces, we studied interactions between the pathogen on lettuce and stainless steel. A panel of 24 strains (23 of Listeria monocytogenes...
Fri, 01/08/2021 - 04:02
Refugee camps provide basic necessities such as food, water, shelter, and medical treatment for displaced persons. Unsanitary conditions in refugee camps due to overcrowding, poor sanitation systems, lack of clean water, minimal ways to cook and store food can lead to an increased risk of foodborne illness. This article reviews the limited literature on the epidemiology of foodborne illness in...
Fri, 01/08/2021 - 04:02
Salmonella enterica has been increasingly linked to outbreaks involving consumption of fresh produce. While several studies have identified genes whose products are involved in mediating S. enterica -plant interactions, the use of varying experimental approaches, serovars and plant types have generated variable and conflicting data. The purpose of this study was to determine whether conditions...
Fri, 01/08/2021 - 02:03
Paragonimiasis is a foodborne trematode infection that affects 23 million people, mainly in Asia. Lung fluke infections lead frequently to chronic cough with fever and hemoptysis, and are often confused with lung cancer or tuberculosis. Paragonimiasis can be efficiently treated with praziquantel, but diagnosis is often delayed, and patients are frequently treated for other conditions. To improve...
Fri, 01/08/2021 - 02:02
Due to the relatively high tolerance and resistance to sanitizers, biofilms can persist in the environment resulting in cross-contamination. The overall goal of this study was to evaluate the impact of nanobubbles (NB) alone and in combination with neutral electrolyzed water (NEW) on different microbial biofilms including Escherichia coli O157:H7, Vibrio parahaemolyticus, and Listeria innocua on...
Thu, 01/07/2021 - 22:14
Animal petting zoos and farm fairs provide the opportunity for children and adults to interact with animals, but contact with animals carries a risk of exposure to zoonotic pathogens and antimicrobial‐resistant bacteria. The aim of this study was to assess the occurrence of Shiga toxin‐producing Escherichia coli (STEC), Salmonella, extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae...
Thu, 01/07/2021 - 22:14
This work aimed at studying the unconfirmed hypothesis predicting the existence of a connection between coffee farm microbiome and the resulting spontaneous fermentation process. Using Illumina‐based amplicon sequencing, 360 prokaryotes and 397 eukaryotes were identified from coffee fruits and leaves, over‐ripe fruits, water used for coffee de‐pulping, depulped coffee beans, soil, and temporal...
Thu, 01/07/2021 - 16:00
Formation of viable but non-culturable (VBNC) status in methicillin-resistant Staphylococcus aureus (MRSA) has never been reported, and it poses a significant concern for food safety. Thus, this study aimed to firstly develop a rapid, cost-effective, and efficient testing method to detect and differentiate MRSA strains in the VBNC state and further apply this in real food samples. Two targets...
Thu, 01/07/2021 - 10:05
ABSTRACT Salmonella enterica encodes a wide array of virulence factors. One novel virulence factor, an A2B5 toxin known as the typhoid toxin (TT), was recently identified among a variety of S. enterica serovars. While past studies have shown that some serovars encode both the TT (active subunits CdtB and PltA and binding subunit PltB) and a second binding subunit (ArtB), these serovars were...
Thu, 01/07/2021 - 10:05
ABSTRACT Escherichia coli is a common bacterial species in the gastrointestinal tracts of warm-blooded animals and humans. Pathogenicity and antimicrobial resistance in E. coli may emerge via host switching from animal reservoirs. Despite its potential clinical importance, knowledge of the population structure of commensal E. coli within wild hosts and the epidemiological links between E. coli in...
Thu, 01/07/2021 - 10:05
Enterohemorrhagic Escherichia coli (EHEC) isolates of serotype O157:H7 are serious foodborne zoonotic pathogens and prime targets for biocontrol using bacteriophages. We report on the complete genome sequences of 11 novel lytic bacteriophages, representing three viral genera, isolated from cattle in Hungary that target E. coli O157 strains.
Thu, 01/07/2021 - 10:05
Salmonella enterica serovar Livingstone 1236H was isolated originally from peanut butter and represents a health risk in low-moisture foods. The current work presents the strain’s genome sequencing results, which show a 4,824,729-bp genome sequence and 4,435 protein coding sequences, including some that are involved in adaptation to low-moisture environments.
Thu, 01/07/2021 - 10:05
Bacteriophages can be used as a biocontrol for the foodborne pathogen Listeria monocytogenes. Propagation of phages is a necessary step for their use in experimental studies and biocontrol applications. Here, we present the complete genomes of three Listeria monocytogenes strains commonly used as propagation hosts for Listeria phages.
Thu, 01/07/2021 - 10:05
Listeria monocytogenes serotype 7 lacks glycosidic constituents in wall teichoic acids. Here, we present the complete genome sequence of L. monocytogenes serotype 7 strain FSL R9-0915 and an analysis of genes known to affect L. monocytogenes antigenicity. This strain is used as a control strain in Listeria phage host range analyses.
Thu, 01/07/2021 - 10:05
We report the complete genome (3.9-Mb chromosome, 5.9-kb plasmid) of Clostridium botulinum CJ0611A1, a type A(B) strain isolated from carrot juice distributed in Canada and linked to an international 2006 foodborne botulism outbreak. This strain encodes a full-length bont/A1 gene and a truncated bont/B gene.
Thu, 01/07/2021 - 10:05
Here, we report the complete genome sequences for 36 Canadian isolates of Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant I 1,4,[5]:12:i:– from both clinical and animal sources. These genome sequences will provide useful references for understanding the genetic variation within this prominent serotype.
Thu, 01/07/2021 - 06:15
Author(s): Yu-gang Shi, Run-run Zhang, Chen-min Zhu, Ming-feng Xu, Qing Gu, Rammile Ettelaie, Shan Lin, Yi-fan Wang, Xin-yi Leng The objective of this study was to investigate the antibacterial activity and potential mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus. Results show that the length of the alkyl chain plays a pivotal role in eliciting the activity and...
Thu, 01/07/2021 - 06:14
Author(s): Na Ling, Xin Wang, Dengyu Liu, Yizhong Shen, Danfeng Zhang, Dexin Ou, Hongying Fan, Juan Wang, Yu Ding, Jumei Zhang, Qingping Wu, Yingwang Ye Cronobacter malonaticus is one of the important foodborne pathogens causing infections mainly in adults. Biofilm formation, adhesion, and motility in Cronobacter have been documented, but the implying molecular mechanism has received little...
Thu, 01/07/2021 - 02:02
Abstract Vibrio parahaemolyticus is one of the major pathogens responsible for vibriosis and zoonotic infections in teleosts, marine invertebrates, and also humans through consumption of contaminated or unprocessed seafood. Emergence of resistance against current accessible antibiotics and spread to the food chain and environment necessitate the development of safe and effective subunit vaccine...
Thu, 01/07/2021 - 02:02
Active films have gained increasing interest because of their potential to reduce the amount of additives into foods. The aim of this work was to produce and characterize bi-layer films based on gelatin in both layers, in which the nisin and/or “pitanga” leaf hydroethanolic extract was loaded in the second layer. The bi-layer gelatin-based films were characterized regarding humidity and...
Thu, 01/07/2021 - 00:00
Over the last few decades, proteins and peptides have become increasingly more common as FDA-approved drugs, despite their inefficient delivery due to their inability to cross the plasma membrane. In this context, bacterial two-component systems, termed AB toxins, use various protein-based membrane translocation mechanisms to deliver toxins into cells, and these mechanisms could provide new...
Wed, 01/06/2021 - 16:00
The presence of Listeria monocytogenes in Mozzarella di Bufala Campana Protected Designation of Origin cheeses may depend on curd stretching conditions and post contaminations before packaging. To avoid cross-contamination, thermal treatment of water, brines and covering liquid may become necessary. The present study aimed to improve knowledge about L. monocytogenes thermal resistance focusing on...
Wed, 01/06/2021 - 14:04
The purpose of this research was to characterize Listeria monocytogenes from several environmental and clinical sources and assess the efficacy of single and combined physico-chemical treatments in reducing biofilm on lettuce leaves. PCR analysis of L. monocytogenes isolates collected from different clinical (10 strains) and environmental sources (12 strains) was used to look for the presence of...
Wed, 01/06/2021 - 10:02
ABSTRACT Pullorum disease is one of the most common diarrhea-related diseases caused by Salmonella enterica subspecies enterica serovar Gallinarum biovar Pullorum (S. Pullorum); it negatively affects the poultry industry. However, limited studies have explored the association between the gut microbiota and S. Pullorum infection in chickens. In the present study, we performed a microbiome...
Wed, 01/06/2021 - 08:02
by Lena Diaz, Yong Li, Daniel M. Jenkins Constraints related to sample preparation are some of the primary obstacles to widespread deployment of molecular diagnostics for rapid detection of trace quantities (≤103 CFU/mL) of food-borne pathogens. In this research, we report a sample preparation method using a novel handheld electroflotation system to concentrate and recover dilute quantities (102−...
Wed, 01/06/2021 - 06:09
Author(s): Mario González-Angulo, Vinicio Serment-Moreno, Laura Clemente-García, Carole Tonello, Isabel Jaime, Jordi Rovira Despite the commercial success of high pressure processing (HPP) in the juice industry and the fact that no foodborne outbreaks have been reported so far, some regulatory agencies still require process validation. However, there is a lack of consensus on various aspects...
Wed, 01/06/2021 - 06:09
Author(s): Xin Wang, Yiluo Cheng, Wenting Zhang, Qin Lu, Guoyuan Wen, Qingping Luo, Huabin Shao, Zishu Pan, Tengfei Zhang Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host...
Wed, 01/06/2021 - 06:08
Author(s): Adrián Alvarez-Molina, José F. Cobo-Díaz, Mercedes López, Miguel Prieto, María de Toro, Avelino Alvarez-Ordóñez The food processing environments of a newly opened meat processing facility were sampled in ten visits carried out during its first 1.5 years of activity and analyzed for the presence of Listeria monocytogenes. A total of 18 L. monocytogenes isolates were obtained from 229...
Wed, 01/06/2021 - 06:08
Author(s): Xin Yin, Nkuchia M. M’ikanatha, Epiphanie Nyirabahizi, Patrick F. McDermott, Heather Tate Antimicrobial resistance (AMR) in non-typhoidal Salmonella from poultry is a public health concern. Injudicious use of antibiotics in humans and agriculture fuels the emergence of resistance. The objective of this study is to characterize the prevalence, antibiotic susceptibility profiles and...
Wed, 01/06/2021 - 06:08
Author(s): Tania Veltman, David Jordan, Christopher A. McDevitt, Jan Bell, Benjamin P. Howden, Mary Valcanis, Mark O’Dea, Sam Abraham, Peter Scott, Jessica H. Kovac, Raymond Chia, Barry Combs, Kapil Chousalkar, Timothy Wilson, Darren J. Trott The development of antimicrobial resistance in foodborne pathogens is a growing public health concern. This study was undertaken to determine the...
Wed, 01/06/2021 - 06:07
Author(s): Zahra Nooreh, Kamran Taherpour, Mohammad Akbari Gharaei, Hassan Shirzadi, Hossein Ali Ghasemi Colonization of the gastrointestinal tract by potentially pathogenic bacteria and their shedding in animal feces is a fundamental factor for both animal health and human food safety. This study was conducted to evaluate the efficacy of salinomycin, direct-fed microbial, and Ferulago angulata...
Wed, 01/06/2021 - 06:07
Author(s): Joshua M. Lyte, Sandip Shrestha, Basanta R. Wagle, Rohana Liyanage, Diego A. Martinez, Annie M. Donoghue, Karrie M. Daniels, Mark Lyte Microbial endocrinology, which is the study of neurochemical-based host-microbe interaction, has demonstrated that neurochemicals affect bacterial pathogenicity. A variety of neurochemicals, including norepinephrine, were shown to enhance intestinal...
Wed, 01/06/2021 - 02:03
The study evaluates the effect of Artemisia dracunculus essential oil (EO) on two pathogenic bacteria Salmonella enterica serovar Typhimurium and Staphylococcus aureus and Vero cell line. To evaluating the anti-biofilm potential of the EO, a microtiter-plate test (MtP) and scanning electron microscopy (SEM) were performed. The quorum-sensing inhibitory properties were examined by QS-related gene...
Wed, 01/06/2021 - 02:02
In this study, a real-time loop-mediated isothermal amplification (Rti-LAMP) combined with immunomagnetic beads separation (IMS) and ethidium bromide monoazide (EMA) treatment was developed for the rapid and ultrasensitive detection of viable Escherichia coli (E. coli) O157:H7 in milk without enrichment. Polyclonal antibodies against E. coli O157:H7 flagellum and specific immunomagnetic beads (...
Wed, 01/06/2021 - 02:02
In recent years, there has been a serious need to develop and test new biodegradable packaging. Therefore, furcellaran (FUR) and whey protein isolate (WPI) films containing pu-erh (PE) or green tea (GT) extracts were prepared in this study. The effect of the extracts on the structure, rheological and mechanical properties, and antioxidant and antimicrobial activity of FUR/WPI films was tested....

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