Bacterial pathogens

Fri, 11/06/2020 - 16:00
Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO2/Au) nanosphere (NP) with...
Fri, 11/06/2020 - 16:00
Evolution experiments in the laboratory have focused heavily on model organisms, often to the exclusion of clinically relevant pathogens. The foodborne bacterial pathogen Campylobacter jejuni belongs to a genus whose genomes are small compared to those of its closest genomic relative, the free-living genus Sulfurospirillum, suggesting genome reduction during the course of evolution to host...
Fri, 11/06/2020 - 16:00
As an important foodborne pathogen, Vibrio vulnificus gives a significant threat to food safety and public health. Rapid and accurate detection methods for V. vulnificus are required to control its spread. The conventional detection methods are time-consuming and labor-intensive, while the polymerase chain reaction (PCR)- and quantitative PCR (qPCR)-based methods are limited because of their...
Fri, 11/06/2020 - 16:00
Chironomids (Diptera; Chironomidae), also known as non-biting midges, are one of the most abundant insects in freshwater habitats. Our aim was to understand whether the metamorphosis developmental stages affect the endogenous microbiota composition of Chironomus transvaalensis. Toward our objective, we analyzed the endogenous microbiota composition of C. transvaalensis’ four life stages: egg...
Fri, 11/06/2020 - 12:02
ABSTRACT The Gram-negative bacterium, Aeromonas hydrophila, has been responsible for extensive losses in the catfish industry for over a decade. Due to this impact, there are ongoing efforts to understand the basic mechanisms that contribute to virulent A. hydrophila (vAh) outbreaks. Recent challenge models demonstrated that vAh cultured in the presence of the iron chelating agent deferoxamine...
Fri, 11/06/2020 - 08:02
Nontuberculous mycobacteria (NTM) compose a group of mycobacteria that do not belong to the Mycobacterium tuberculosis complex group. They are frequently isolated from environmental samples such as water, soil, and, to a lesser extent, food samples. Isolates of NTM represent a major health threat to humans worldwide, especially those who have asthma or are immunocompromised. Human disease is...
Fri, 11/06/2020 - 08:02
We identified 199 Staphylococcus aureus isolates from quarter milk samples of 1,289 dairy cattle between 2014 and 2018. About 66% of the isolates were resistant to at least 1 antimicrobial agent; the highest rate of resistance was to penicillin, followed by resistance to ampicillin, erythromycin, and sulfadimethoxine. We obtained 30 methicillin-resistant S. aureus (MRSA) strains from 6 farms in 3...
Fri, 11/06/2020 - 06:18
Author(s): Laurent Guillier, Philippe Fravalo, Alexandre Leclercq, Anne Thébaut, Pauline Kooh, Vasco Cadavez, Ursula Gonzales-Barron Yersinia enterocolotica is an important causative agent of diarrheal illness. A systematic review and meta-analysis of case-control studies were performed to determine the main risk factors associated with sporadic Y. enterocolitica infections. Suitable scientific...
Fri, 11/06/2020 - 06:14
Author(s): Tareq M. Osaili, Fayeza Hasan, Dinesh Kumar Dhanasekaran, Reyad S. Obaid, Anas A. Al-Nabulsi, Mutamed Ayyash, Layal Karam, Ioannis N. Savvaidis, Richard Holley The objective of the study was to assess the antimicrobial effect of active essential oil components (EOs) namely (carvacrol (CA), cinnamaldehyde (CI) and thymol (TH)) on Listeria monocytogenes, Salmonella spp., and Escherichia...
Fri, 11/06/2020 - 06:14
Author(s): Katherine M. Costello, Cindy Smet, Jorge Gutierrez-Merino, Madeleine Bussemaker, Jan F. Van Impe, Eirini G. Velliou Novel processing methods such as cold atmospheric plasma (CAP) and natural antimicrobials like nisin, are of interest to replace traditional food decontamination approaches as, due to their mild nature, they can maintain desirable food characteristics, i.e., taste,...
Fri, 11/06/2020 - 06:14
Author(s): Veronica Martinez-Rios, Mikael Pedersen, Monica Pedrazzi, Elissavet Gkogka, Jørn Smedsgaard, Paw Dalgaard This study tested the hypothesis that growth of Listeria monocytogenes in processed cheese with added nisin can be predicted from residual nisin A concentrations in the final product after processing. A LC-MS/MS method and a bioassay were studied to quantify residual nisin A...
Fri, 11/06/2020 - 06:13
Author(s): Ross C. Beier, J. Allen Byrd, Kathleen Andrews, Denise Caldwell, Tawni L. Crippen, Robin C. Anderson, David J. Nisbet Foodborne illness is an on-going problem worldwide and is caused by bacteria that invade the food chain from the farm, slaughter house, restaurant or grocery, or in the home and can be controlled by strategies using biocides (antiseptics and disinfectants)....
Fri, 11/06/2020 - 06:13
LWT
Author(s): A. Arun Prince Milton, Kasanchi M. Momin, Sandeep Ghatak, Shiny C. Thomas, G. Bhuvana Priya, M. Angappan, Samir Das, R.K. Sanjukta, K. Puro, I. Shakuntala, A. Sen, B.K. Kandpal Staphylococcus aureus is one of the most common and leading causes of food poisoning and also responsible for multifarious diseases in humans. Rapid and on-site detection of S. aureus is of specific importance...
Fri, 11/06/2020 - 04:03
Lytic polysaccharide monooxygenases (LPMOs) are redox-active enzymes that cleave insoluble polysaccharides by an oxidative reaction. In the present study, we have characterized four recombinant putative chitin-active LPMOs from Streptomyces griseus (SgLPMO10B, -C, -D, and -F) and evaluated their potential in enhancing hydrolysis of α- and β-chitin by three families of 18 chitinases of Serratia...
Fri, 11/06/2020 - 02:04
Despite significant advances in understanding the role of the immune response in Opisthorchis viverrini-associated carcinogenesis, little is known about how infection induces gall bladder disease. This study investigated whether mast cells are activated in cholecystitis associated with O. viverrini, gall bladder specimens from ninety-two patients who had undergone cholecystectomy at the Khon Kaen...
Thu, 11/05/2020 - 22:15
Aims This study compared the capacity of strains of Salmonella enterica serovars Enteritidis and Dublin isolated in Brazil to invade epithelial cells, to be internalized by and survive within macrophages, and to stimulate cytokine release in vitro. Methods and Results Both serovars infected 75% and 73% Caco‐2 (human) and MDBK (bovine) epithelial cells, respectively. S. Dublin and S. Enteritidis (...
Thu, 11/05/2020 - 16:00
As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter jejuni has a significant impact on human health in both the developed and developing worlds. Despite its prevalence as a human pathogen, the source of these infections remains poorly understood due to the mutation frequency of the organism and past limitations of whole genome analysis. Recent advances in both whole...
Thu, 11/05/2020 - 16:00
Every year millions of people die due to fatal waterborne diseases around the world especially in developing countries like India. Sikkim, a northeastern state of India, greatly depends on natural water sources. About 80% of the population of Sikkim depends on natural spring water for domestic as well as agricultural use. Recent waterborne disease outbreaks in the state raises a concerning...
Thu, 11/05/2020 - 16:00
Autophagy is a fundamental and highly conserved eukaryotic process, responsible for maintaining cellular homeostasis and releasing nutrients during times of starvation. An increasingly important function of autophagy is its role in the cell autonomous immune response; a process known as xenophagy. Intracellular pathogens are engulfed by autophagosomes and targeted to lysosomes to eliminate the...
Thu, 11/05/2020 - 12:01
Background Campylobacter species are among the most common causes of enteric bacterial infections worldwide. Men who have sex with men (MSM) are at increased risk for sexually transmitted enteric infections, including globally distributed strains of multidrug-resistant Shigella species. Methods This was a retrospective study of MSM-associated Campylobacter in Seattle, Washington and Montréal,...
Thu, 11/05/2020 - 12:01
Background Produce-associated outbreaks of Shiga toxin–producing Escherichia coli (STEC) were first identified in 1991. In April 2018, New Jersey and Pennsylvania officials reported a cluster of STEC O157 infections associated with multiple locations of a restaurant chain. The Centers for Disease Control and Prevention (CDC) queried PulseNet, the national laboratory network for foodborne disease...
Thu, 11/05/2020 - 12:01
Background Typhoid fever caused by Salmonella Typhi is a major public health concern in low-/middle-income countries. A recent study of 1900 global S. Typhi indicated that South Asia might be the site of the original emergence of the most successful and hypervirulent clone belonging to the 4.3.1 genotype. However, this study had limited samples from India. Methods We analyzed 194 clinical S....
Thu, 11/05/2020 - 10:03
Shiga toxin-producing Escherichia coli (STEC) serotype O103 is one of the primary pathogenic contaminants of beef products, contributing to several foodborne outbreaks in recent years. Here, we report the whole-genome sequence of a STEC O103:H2 strain isolated from cattle feces that contains a locus of enterocyte effacement (LEE) pathogenicity island.
Thu, 11/05/2020 - 10:03
In nature, the fruit fly frequently encounters various pathogens that cause a decrease in host fitness. Here, we present the genome sequence of Serratia marcescens strain FY, which was isolated from the intestines of a Drosophila specimen. The complete genome sequence comprises one chromosome of 5,074,453 bp and two plasmids of 100,934 bp and 87,789 bp. A total of 4,891 coding sequences are...
Thu, 11/05/2020 - 08:03
by Ngo Ngoc Quang Minh, Pham Van Toi, Le Minh Qui, Le Binh Bao Tinh, Nguyen Thi Ngoc, Le Thi Ngoc Kim, Nguyen Hanh Uyen, Vu Thi Ty Hang, Nguyen Thi Thuy Chinh B’Krong, Nguyen Thi Tham, Thai Dang Khoa, Huynh Duy Khuong, Pham Quynh Vi, Nguyen Ngoc Hong Phuc, Le Thi Minh Vien, Thomas Pouplin, Doan Van Khanh, Pham Nguyen Phuong, Phung Khanh Lam, Heiman F. L. Wertheim, James I. Campbell, Stephen Baker...
Thu, 11/05/2020 - 06:26
Author(s): Qichao Sun, Yun Lv, Chenhui Zhang, Weifang Wu, Rui Zhang, Chunyuan Zhu, Yao-yao Li, Huiqing Yuan, Jing Zhu, Deyu Zhu Cyclic di-AMP is a bacterial nucleotide second messenger and evaluated as a potential vaccine adjuvant candidate. Here, we report a practical and economical enzymatic method for gram-scale preparation of c-di-AMP using an immobilized Vibrio cholerae dinucleotide cyclase...
Thu, 11/05/2020 - 00:00
Salmonella enterica is one of the most common bacterial foodborne pathogens in the United States, causing illnesses that range from self-limiting gastroenteritis to more severe, life threatening invasive disease. Many Salmonella strains contain plasmids that carry virulence, antimicrobial resistance, and/or transfer genes which allow them to adapt to diverse environments, and these can include...
Wed, 11/04/2020 - 10:03
To initiate their life cycle, phages must specifically bind to the surface of their bacterial hosts. Long-tailed phages often interact with the cell surface using fibers, which are elongated intertwined trimeric structures. The folding and assembly of these complex structures generally requires the activity of an intra- or intermolecular chaperone protein. Tail fiber assembly (Tfa) proteins are a...
Wed, 11/04/2020 - 06:18
Author(s): Tassiana Ramires, Natalie Rauber Kleinubing, Mariana Almeida Iglesias, Helena Reissig Soares Vitola, Adriana Souto Pereira Núncio, Isabela Schneid Kroning, Gustavo Marçal Schmidt Garcia Moreira, Ângela Maria Fiorentini, Wladimir Padilha da Silva Sushi is a ready-to-eat (RTE) food prepared from raw or cooked fish that is widely consumed worldwide. Listeria monocytogenes is the foodborne...
Wed, 11/04/2020 - 06:13
Author(s): Fangshen Guo, Fangyuan Wang, Haiyan Ma, Zhouzheng Ren, Xiaojun Yang, Xin Yang With global warming and ban on antibiotics, it occurs occasionally that deoxynivalenol (DON) together with Clostridium perfringens impairs the gut health of broiler chickens. However, the interactive effect of DON and C. perfringens on intestinal health is still unknown. A total of 120 1-day-old Arbor Acres...
Tue, 11/03/2020 - 22:14
BACKGROUND Eleven Lactobacillus (L.) strains were newly isolated from traditional Turkish Tulum cheeses and were characterized regarding their potential probiotic characteristics (bile and acid tolerance, gastric and pancreatic juice tolerance, lysozyme tolerance, adhesion ability), virulence determinants (haemolytic activity, antibiotic resistance, biogenic amine production), and functional...
Tue, 11/03/2020 - 22:14
BACKGROUND Dietary intervention is an important approach to improve intestinal function of weaned piglets. Phytogenic and herbal products have received increasing attention as in‐feed antibiotic alternatives. This study investigated the chemical composition of guava leaf extract (GE) by ultrahigh‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS). Meanwhile, we investigated...
Tue, 11/03/2020 - 16:00
Colibacillosis is an economically important infectious disease in poultry, caused by avian pathogenic Escherichia coli (APEC). Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major cause of food-borne diseases in human circulated through poultry-derived products, including meat and chicken eggs. Vaccine control is the mainstream approach for combating these infections, but it is...
Tue, 11/03/2020 - 16:00
An effective environmental sampling method involves the use of a transport/neutralizing broth with the ability to neutralize sanitizer residues that are collected during sampling and to maintain viability of s...
Tue, 11/03/2020 - 06:37
Author(s): Barun Yadav, M.S. Roopesh Recently, Salmonella has been linked to numerous low water activity (aw) food recalls. As an emerging technology, atmospheric cold plasma (ACP) has the potential to reduce Salmonella in low aw foods. The objective of this study was to evaluate the effects of initial inoculum population of Salmonella (~ 10, 9 and 8 log CFU/cm2), treatment time (2.5, 5, 7.5 and...
Tue, 11/03/2020 - 06:26
LWT
Author(s): Pabasara Weerarathne, Josh Payne, Joyjit Saha, Tony Kountoupis, Ravi Jadeja, Divya Jaroni Escherichia coli O157:H7 biofilms pose challenges to the food industry by facilitating pathogen persistence. Sodium acid sulfate (SAS), a food-acid with antimicrobial properties, is poorly understood for its biofilm-disrupting capabilities. Efficacy of SAS (0.1, 1, and 3%), to disrupt E. coli O157...
Tue, 11/03/2020 - 06:26
LWT
Author(s): Hou-Qi Ning, Ying-Qiu Li, Hong Lin, Jing-Xue Wang ε-poly-lysine (ε-PL) is a safe and excellent natural preservative. This study aimed to investigate the antibacterial actions of ε-PL against Staphylococcus aureus (S. aureus) and its anti-S. aureus activity on the pasteurized milk. Electron microscopy observation showed ε-PL severely disrupted the cell wall and membrane of S. aureus,...
Tue, 11/03/2020 - 02:02
In this study, light intensities of Aliivibrio fischeri obtained from soil suspension and its filtrate were compared using field soils contaminated with heavy metals. The soils collected from rice paddy and forest had different soil textures with either high or low silt/clay content. The correlation between soil toxicity and soil solution toxicity for A. fischeri showed a high linearity in sandy...
Tue, 11/03/2020 - 00:00
Altogether, 2547 Staphylococcus aureus isolated from cattle (n = 382), pig (n = 1077), and chicken carcasses (n = 1088) during 2010–2017 were investigated for linezolid resistance and were further characterized using molecular methods. We identified linezolid resistance in only 2.3% of pig carcass isolates. The linezolid-resistant (LR) isolates presented resistance to multiple...
Tue, 11/03/2020 - 00:00
Diseases caused by Escherichia coli (E. coli) and Salmonella spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) E. coli and Salmonella spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial...
Mon, 11/02/2020 - 16:00
Salmonella Typhimurium expresses on its outer membrane the protein Rck which interacts with the epidermal growth factor receptor (EGFR) of the plasma membrane of the targeted host cells. This interaction activates signaling pathways, leading to the internalization of Salmonella. Since EGFR plays a key role in cell proliferation, we sought to determine the influence of Rck mediated infection on...
Mon, 11/02/2020 - 14:08
Infectious diarrhea causes approximately 179 million illnesses annually in the US. Multiplex PCR assays for enteric pathogens detect enteropathogenic Escherichia coli (EPEC) in 12–29% of diarrheal stool samples from all age groups in developed nations. The aim of this study was to isolate and characterize EPEC from diarrhea samples identified as EPEC positive by BioFire Gastrointestinal Panel (...
Mon, 11/02/2020 - 08:02
Background. The issue of microbial quality and safety of ready-to-eat foods has become a public health concern that needs to be addressed to protect the consumer’s health. Contamination of ready-to-eat foods by enteric pathogens such as Escherichia coli, Salmonella, and Staphylococcus aureus bacteria is associated with potential health risks and can cause foodborne outbreaks. Thus, the systematic...
Mon, 11/02/2020 - 04:02
With the rising prevalence of multidrug resistance, there is an urgent need to develop novel antibiotics. Many putative antibiotics demonstrate promising in vitro potency but fail in vivo due to poor drug-like qualities (e.g., serum half-life, oral absorption, solubility, and toxicity). These drug-like properties can be modified through the addition of chemical protecting groups, creating “...
Mon, 11/02/2020 - 04:02
Enterohemorrhagic Escherichia coli (EHEC) is the causative agent of severe diarrheal disease in humans. Cattle are the natural reservoir of EHEC, and approximately 75% of EHEC infections in humans stem from bovine products. Many common bacterial pathogens, including EHEC, rely on chemical communication systems, such as quorum sensing (QS), to regulate virulence and facilitate host colonization....
Mon, 11/02/2020 - 00:00
We studied the presence of the mobile colistin resistance gene mcr-1 in Escherichia coli isolates recovered from fecal and urine samples of companion animals, that were collected from South Korea in 2018 and 2019. The mcr-1 gene was detected in one colistin-resistant E. coli isolated from a diarrheic dog. The isolate exhibited additional resistance to multiple antimicrobials, including...
Sun, 11/01/2020 - 06:13
Author(s): Qiao He, Mingming Guo, Tony Z. Jin, Saifanassour Ali Arabi, Donghong Liu Development of novel and effective decontamination technologies to ensure the microbiological safety of fresh produce has gained considerable attention, mainly driven by numerous outbreaks. This work presented the first approach regarding to the application of the previously reported hurdle technologies on the...
Sun, 11/01/2020 - 06:13
Author(s): Takako Taniguchi, Mayuko Ohki, Ayaka Urata, Shoutaro Ohshiro, Elpita Tarigan, Savek Kiatsomphob, Torrung Vetchapitak, Hiroyuki Sato, Naoaki Misawa Campylobacter jejuni is the leading cause of bacterial food poisoning worldwide. Chickens are considered to be one of the major reservoirs of Campylobacter infection in humans due to colonization of their intestinal tract. When the chickens...
Sun, 11/01/2020 - 00:00
Sialorrhea, or excessive saliva beyond the margin of the lip, is a common problem in many neurological diseases. Previously, sialorrhea has been underrecognized in Parkinson’s disease (PD) patients. Despite this, many patients rank sialorrhea as one of the most debilitating complaints of Parkinson’s disease. Previous treatment for sialorrhea has been suboptimal and has been...
Sun, 11/01/2020 - 00:00
Salmonella spp. represent a public health concern for humans and animals due to the increase of antibiotic resistances. In this scenario, the use of essential oils (EOs) could be a valid tool against Salmonella contamination of meat. This work compares the in vitro effectiveness of an Italian mixture of feed additives based on EOs (GR-OLI) with EO of Origanum vulgare L., recently admitted by...

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