Bacterial pathogens

Tue, 10/29/2019 - 03:34
Author(s): Yongmei Guo, Christopher McMullen, Edouard Timsit, Jennyka Hallewell, Karin Orsel, Frank van der Meer, Sumei Yan, Trevor W. AlexanderAbstractRecent studies have shown an increase in antimicrobial-resistant bovine respiratory disease (BRD) pathogens. To investigate the origin of antimicrobial resistance in the respiratory microbiota of beef cattle, three groups (A, B, or C) of 40 calves...
Tue, 10/29/2019 - 03:34
Author(s): Miaomiao Liu, Mingxing Feng, Kun Yang, Youfang Cao, Jie Zhang, Junnan Xu, Santiago Hernández Hernández, Xinyuan Wei, Mingtao FanAbstractPersimmon tannin (PT) exhibits antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) isolated from retail pork. The involved molecular mechanisms were investigated for the first time using transcriptome and metabolome in...
Tue, 10/29/2019 - 03:30
The above article from the Journal of Food Safety, published online on November 23, 2018 in Wiley Online Library (https://onlinelibrary.wiley.com/doi/full/10.1111/jfs.12573), has been retracted by agreement between the journal Editor‐in‐Chief, Vivian Wu and Wiley Periodicals, Inc. The retraction has been agreed due to evidence indicating that the peer review of this paper was compromised and...
Tue, 10/29/2019 - 03:30
The above article from the Journal of Food Safety, published online on October 22, 2018 in Wiley Online Library (https://onlinelibrary.wiley.com/doi/full/10.1111/jfs.12501), has been retracted by agreement between the journal Editor‐in‐Chief, Vivian Wu and Wiley Periodicals, Inc. The retraction has been agreed due to evidence indicating that the peer review of this paper was compromised and...
Tue, 10/29/2019 - 03:30
Essential oils (EOs) are natural substances extracted from aromatic and medicinal plants (AMPs), important in food preservation. Several studies have shown that AMPs, as well as their EOs have antimicrobial (antibacterial and antifungal) activity. Indeed, our in vitro studies have shown that oregano and thyme EOs are effective against foodborne bacteria, isolated from fermented meat products and...
Tue, 10/29/2019 - 03:30
In this study, it is aimed to investigate the potential effects of the use of quinoa as a carrier polymer in the production of edible film and the importance of essential oils (with lemon and sage oil) with highly important biological activity as supportive in this coating material. The rainbow trout fillets were wrapped with the edible film which were produced for this trial. The treatment...
Tue, 10/29/2019 - 03:30
The objective of this study was to evaluate the impact of ozone treatment on microbiological decontamination (intrinsic microflora and inoculated Listeria innocua) and some physicochemical characteristics and bioactive compounds of Cantaloupe melon juice, also during refrigerated storage. To determine adequate ozone exposure, the survival curve of L. innocua was previously assessed. A thermal...
Tue, 10/29/2019 - 03:27
In total, 77 rhizobial strains isolated from the root nodules of T. repens, inhabiting heavy metal-contaminated waste heap (36 isolates) and control grassland (41 ones) in southern Poland, were analyzed for genome polymorphism and strength of the heavy metals’ (mainly Zn, Pb, Cd) selective pressure on bacterial genome polymorphism using two PCR-based techniques, ERIC- (enterobacterial repetitive...
Tue, 10/29/2019 - 03:26
The intracellular bacterium Coxiella burnetii is responsible for Q fever, an infectious disease that increases the risk of abortion, preterm labor, and stillbirth in pregnant women. It has been shown that C. burnetii replicates in BeWo trophoblast cell line and inhibits the activation and maturation of decidual dendritic cells. Although tissue macrophages are known to be targeted by C. burnetii,...
Sun, 10/27/2019 - 03:49
Author(s): Qiying Wang, Hao Li, Kaimin Zhou, Xiang Qin, Qun Wang, Weiwei LiAbstractThe Rab family is the most significant subfamily of small GTP-binding proteins. These proteins have widespread intracellular localization and play an important role in many biological processes. Rab7 plays a crucial role in the innate immune system of crustaceans. In the present study, we cloned and characterized...
Sun, 10/27/2019 - 03:48
Author(s): Jinzhong Niu, Yu Huang, Xinchao Liu, Guoling Luo, Jufen Tang, Bei Wang, Yishan Lu, Jia Cai, Jichang JianAbstractGalectin-3 is a kind of β-galactoside-binding lectin involved in host defense against pathogen infection. However, the immune functions of fish galectin-3 remain poorly understood. In this study, the roles of a fish galectin-3 (OnGal-3) from Nile tilapia (Oreochromis...
Sun, 10/27/2019 - 03:47
Author(s): Jingwen Gao, Karl R. MatthewsAbstractThis study investigated the antimicrobial efficacy of the water-soluble photosensitizer curcumin (PSC) on liquid media and chicken skin. The water-soluble PSC showed strong absorption at 410 nm, and the light-emitting diode (LED) used to activate PSC had strong emission at 430 nm with power density of 107 W/m2. Listeria monocytogenes (3 isolates)...
Sun, 10/27/2019 - 03:46
Author(s): Yurim Cho, Hoikyung Kim, Larry R. Beuchat, Jee-Hoon RyuAbstractWe investigated combinations of gaseous essential oils (EO gases) for their synergistic inhibitory activities against Listeria monocytogenes on a laboratory medium and radish sprouts. The minimum inhibitory concentrations and minimum lethal concentrations of oregano, thyme thymol, and cinnamon bark EO gases against L....
Sun, 10/27/2019 - 03:46
Author(s): Ângela Alves, Rui Magalhães, Teresa R.S. Brandão, Lígia Pimentel, Luis M. Rodríguez-Alcalá, Paula Teixeira, Vânia FerreiraAbstractThe objective of this work was to investigate the effect of stress conditions frequently encountered in food-associated environments on virulence-associated characteristics of eight strains of Listeria monocytogenes. Strains were grown at low (11 ºC, cold...
Sun, 10/27/2019 - 03:44
Author(s): David C. Love, Lillian M. Kuehl, Robert M. Lane, Jillian P. Fry, Jamie Harding, Benjamin J.K. Davis, Kate Clancy, Bobbi HudsonAbstractVibrio bacteria can accumulate in molluscan shellfish and cause human diseases. The United States (U.S.) has implemented Vibrio Control Plans to mitigate risks associated with these bacteria, which include time and temperature requirements for post-...
Sun, 10/27/2019 - 03:43
Author(s): Seungbaek Lee, Youngho Kim, Jinhee ChoiAbstractMicrobial community of an organism plays an important role on its fitness, including stress responses. In this study, we investigated the effect of the culturable subset of soil microbial community (SMB) on the stress response of the soil nematode Caenorhabditis elegans, upon exposure to one of the major soil contaminants, cadmium (Cd)....
Sun, 10/27/2019 - 03:43
Author(s): Yuxuan Zhang, Jian Lu, Jun Wu, Jianhua Wang, Yongming LuoAbstractMicroplastics have become emerging pollutants and served as potential vectors for harmful bacteria, while rare information on the emergency and propagation of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) on the surface of microplastics is available. This study investigated the enrichment of...
Sat, 10/26/2019 - 03:50
by Po-Yao Chuang, Tien-Yu Yang, Tsan-Wen Huang, Yao-Hung Tsai, Kuo-Chin Huang, Hsu-Huei Weng Background Vibrio vulnificus necrotizing skin and soft tissue infections (VNSSTIs) are associated with a high mortality rate that varies remarkably with host susceptibility. Hepatic disease (HD) is considered the key risk factor for high VNSSTIs incidence and mortality; however, there is limited evidence...
Sat, 10/26/2019 - 03:48
Author(s): M.J. Fraqueza, C. Martins, L.T. Gama, M.H. Fernandes, M.J. Fernandes, M.H.L. Ribeiro, B.R. Hernando, A.S. Barreto, A.J.I. AlfaiaAbstractThe effect of high hydrostatic pressure (HHP) on the microbial load and physical properties of natural casings, red pepper (Capsicum annuum L.) and garlic (Allium sativum) paste was studied. HHP treatments were performed according to a Response Surface...
Sat, 10/26/2019 - 03:48
Author(s): Pan Wu, Yonghe Gu, Rou Zhao, Yaxin Liu, Yanling Wang, Guozhong Lv, Zhenghai Li, Yajing BaoAbstractThe improvement of resistance diseases, antioxidant and nonspecific immunity responses for Darkbarbel catfish by dietary residual pomegranate was investigated. The number of pathogenic bacteria and cumulative mortality of Darkbarbel catfish under 15–45% groups were decreased compared with...
Sat, 10/26/2019 - 03:45
Author(s): Vaigundan Dhayabaran, Divya Chidambaram, Patnam R. KrishnaswamyAbstractLeptospirosis is a recurring global disease of severe illness involving pulmonary and renal involvement in cattle and humans; that needs attention to cure. The major challenge in treating leptospirosis disease is the diagnosis of the disease. The culturing of an organism is a gold standard for confirmation of the...
Sat, 10/26/2019 - 03:45
Author(s): Mustafa Sadek, Laurent Poirel, Patrice NordmannAbstractThis study compared the impact of different approaches, namely non-enrichment, non-selective enrichment, and selective (antibiotic-containing) enrichment steps, for detecting extended-spectrum ß-lactamase producing Enterobacterales (ESBL-E), carbapenemase-producing Enterobacterales (CPE), polymyxin-resistant Enterobacterales (PMR-E...
Sat, 10/26/2019 - 03:36
Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC from which they can contaminate the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7...
Sat, 10/26/2019 - 03:35
Streptococcus agalactiae is a major pathogen causing streptococcosis. To prevent and control this bacterial disease, antagonistic bacteria have become a new research hotspot. This study evaluated the probiotic potential of Bacillus velezensis LF01 strain, which is antagonistic to S. agalactiae. The active compounds produced by LF01 showed antimicrobial activity against a broad spectrum of fish...
Sat, 10/26/2019 - 03:34
Clostridium perfringens (C. perfringens) is Gram-positive anaerobic, spore-forming rod-shaped bacterial pathogen that is widely distributed in nature. This bacterium is known as the causative agent of a foodborne illness and of gas gangrene. While the major virulence factors are the α-toxin and perfringolysin O (PFO) produced by type A strains of C. perfringens, the precise mechanisms of how...
Sat, 10/26/2019 - 03:34
Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-...
Fri, 10/25/2019 - 03:50
Bacteriophages infecting Salmonella enterica subsp. enterica serovar Enteritidis may be used as biocontrol agents in food products or animals for preventing foodborne diseases caused by this pathogen. The complete genome sequence of phage Seafire, a T5-like siphophage infecting S. Enteritidis, is described in this report.
Fri, 10/25/2019 - 03:50
Salmonella enterica serovar Typhimurium is a foodborne pathogen that causes gastroenteritis. Due to increases in antibiotic resistance, bacteriophage therapy may be an alternative method for preventing Salmonella foodborne infections. We report here the complete genome sequence of a T5-like phage, Seabear, which was isolated against S. Typhimurium.
Fri, 10/25/2019 - 03:50
Uropathogenic Escherichia coli (UPEC) strains are responsible for most cases of urinary tract infections worldwide. We present the draft whole-genome sequence of the UPEC 252 strain, which carries the eae gene that encodes the intimin adhesin. Intimin promotes intimate adherence of enteropathogenic E. coli and enterohemorrhagic E. coli to intestinal cells.
Fri, 10/25/2019 - 03:49
This report shows the whole-genome sequence of the multidrug-resistant Salmonella enterica subsp. enterica serovar Infantis strain FARPER-219. Antibiotic resistance genes are found mainly in the plasmid. Our findings show important genetic information that provides an understanding of the recent spread of this serotype in poultry.
Fri, 10/25/2019 - 03:44
Author(s): Tanise Vendruscolo Dalmolin, Alana Mazzetti, Helena Ávila, Julia Kranich, Gabriel Ibrahim Borba Carneiro, Lavinia Nery Villa Stangçer Arend, Guilherme Nardi Becker, Karin Obladen Ferreira, Daiana de Lima-Morales, Afonso Luís Barth, Marcelo PillonettoAbstractRecently it was developed the Colistin Broth Disk Elution test, uses colistin disks as a source of these antibiotics. The aim of...
Fri, 10/25/2019 - 03:44
Author(s): K.L. Hu, X.Q. Yu, J. Chen, J.N. Tang, L.Z. Wang, Y.M. Li, C. TangAbstractThe 3-methyl-butanal and 3-methyl-butanoic acid are known as fingerprint compounds from Staphylococcus aureus. In this study, we investigated production of these two volatile biomarkers and their correlation to S. aureus growth in pork. Both 3-methyl-butanal and 3-methyl-butanoic acid presented high specificity...
Fri, 10/25/2019 - 03:42
LWT
Author(s): Natalia Parma Augusto de Castilho, Svetoslav Dimitrov Todorov, Leandro Licursi Oliveira, Luciano dos Santos Bersot, Luís Augusto NeroAbstractThe aim of the study was to evaluate the inhibitory activity of Lactobacillus curvatus UFV-NPAC1 and its bacteriocin on Listeria monocytogenes in a controlled food matrix. Fresh sausages were prepared and inoculated with different combinations of...
Fri, 10/25/2019 - 03:38
Predicted and experimental values of Staphylococcus aureus during the storage period (log CFU/g).S. aureus can be inactivated in the early periods in probiotic cheese produced by using Lactobacillus rhamnosus and Lactobacillus casei Shirota together with the starter culture. In addition, this work recommends that the proposed. Fourier series‐modeling method provides the possibility to calculate...
Fri, 10/25/2019 - 03:37
Hog production takes place mostly in large concentrated animal feeding operations (CAFOs) where waste is managed by storing in lagoons prior to land application of lagoon liquid. Salmonella, including antibiotic resistant Salmonella, have been found in the farm environment and lagoons. The objective of this research was to determine whether Salmonella resistant to clinically relevant antibiotics...
Fri, 10/25/2019 - 03:34
DNA methylation has been introduced as a promising biomarker for different diseases. Alterations in macrophage DNA methylation status have been documented during Mycobacterium tuberculosis (Mtb) infection. We conducted this study using a human methylation PCR array kit, which comprised a panel of 22 genes in TLR2 signaling pathway, in order to gain insights into epigenetic interactions between...
Fri, 10/25/2019 - 03:33
Botulinum neurotoxin (BoNT) can counteract the highly frequent involuntary muscle contractions and the uncontrolled micturition events that characterize the neurogenic detrusor overactivity (NDO) due to supra-sacral spinal cord lesions. The ability of the toxin to block the neurotransmitter vesicular release causes the reduction of contractions and improves the compliance of the muscle and the...
Fri, 10/25/2019 - 03:33
The aim of this study was to explore the phytochemical composition, heavy metals analysis and the antibacterial activity of six medicinal plants i.e., Terminalia chebula Retz (fruits), Aegle marmelos L., (fruits), Curcuma longa L., (rhizomes), Syzygium aromaticum L., (flower buds), Piper nigrum L., (seeds), Cinnamomum cassia L., (barks) and its two remedial recipes (recipe 1 and 2) used against...
Thu, 10/24/2019 - 03:48
by Sam Abraham, Mark O’Dea, Shafi Sahibzada, Kylie Hewson, Anthony Pavic, Tania Veltman, Rebecca Abraham, Taha Harris, Darren J. Trott, David Jordan The World Health Organisation has defined “highest priority critically important antimicrobials” (CIAs) as those requiring the greatest control during food production. Evidence demonstrating that restricted antimicrobial usage prevents the emergence...
Thu, 10/24/2019 - 03:48
Enterobacteriaceae represent a diverse and medically important family of bacteria that are difficult to identify to the species level using the standard molecular method of 16S rRNA gene sequencing. Prior work has demonstrated the value of dnaJ gene sequence analysis in resolving different members of the family. However, existing protocols are not optimized for clinical use and exhibit several...
Thu, 10/24/2019 - 03:48
Genomic surveillance that combines bacterial sequencing and epidemiological information will become the gold standard for outbreak detection, but its clinical translation is hampered by the lack of automated interpretation tools. We performed a prospective pilot study to evaluate the analysis of methicillin-resistant Staphylococcus aureus (MRSA) genomes using the Next Gen Diagnostics (NGD)...
Thu, 10/24/2019 - 03:48
Pathogen cell-free DNA (pcfDNA) in blood and urine is an attractive biomarker; however, the impact of preanalytical factors is not well understood. Blood and urine samples from healthy donors spiked with cfDNA from Mycobacterium tuberculosis, Salmonella enterica, Aspergillus fumigatus, and Epstein-Barr virus (EBV) and samples from tuberculosis patients were used to evaluate the impact of blood...
Thu, 10/24/2019 - 03:48
Rifampin heteroresistance—where rifampin-resistant and -susceptible tuberculosis (TB) bacilli coexist—may result in failed standard TB treatment and potential spread of rifampin-resistant strains. The detection of rifampin heteroresistance in routine rapid diagnostic tests (RDTs) allows for patients to receive prompt and effective multidrug-resistant-TB treatment and may improve...
Thu, 10/24/2019 - 03:44
Author(s): Marisol Goni-Urriza, Christophe Klopp, Magali Ranchou-Peyruse, Anthony Ranchou-Peyruse, Mathilde Monperrus, Bahia Khalfaoui-Hassani, Rémy GuyoneaudAbstractMercury methylation converts inorganic mercury into the toxic methylmercury, and the consequences of this transformation are worrisome for human health and the environment. This process is performed by anaerobic microorganisms, such...

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