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Bacterial pathogens

Fri, 04/05/2019 - 01:14
Publication date: Available online 4 April 2019Source: LWTAuthor(s): Wilbert Long, Majher I. Sarker, Bassam A. Annous, George C. PaoliAbstractThis study evaluated the efficacy of alkyltrimethylammonium-bromide (ATMB) and chlorhexidine-digluconate (CDG) to reduce pathogens of meat safety concerns in solution and on hides. In solution, ATMB at 50 and 250 ppm reduced individual cocktails of...
Fri, 04/05/2019 - 01:14
Available online 4 April 2019 Author(s): EunSol Her, Soobin Seo, Jihee Choi, Victor Pool, Sanja IlicABSTRACTFood courts are popular dining platform in most universities in the United States. However, insufficiently skilled part-time student employees, self-service and shared dining environment, and the low-risk food safety perception among young adult consumer population create an increased risk...
Fri, 04/05/2019 - 01:13
Yalong Bai, Yan Cui, Yujuan Suo, Chunlei Shi, Dapeng Wang, Xianming Shi
Fri, 04/05/2019 - 01:13
Publication date: Available online 3 April 2019Source: Author(s): Marie-Christine Etty, Sabato D’Auria, Carole Fraschini, Stephane Salmieri, Monique LacroixAbstractThis paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the...
Fri, 04/05/2019 - 00:00
Abstract High dietary salt intake is a risk factor for hypertension and a public health challenge worldwide. The aim of this study was to evaluate the effects of salt substitutes on the safety and quality of selected fish products, i.e., Matjes nordische Art and cold-smoked salmon. Sodium chloride was replaced by various salt substitutes including potassium chloride, potassium...
Thu, 04/04/2019 - 11:00
DNA is the carrier of genetic information. DNA modifications play a central role in essential physiological processes. Phosphorothioation (PT) modification involves the replacement of an oxygen atom on the DNA backbone with a sulfur atom. PT modification can cause genomic instability in Salmonella enterica under hypochlorous acid stress. This modification restores hydrogen peroxide (H2O2)...
Thu, 04/04/2019 - 11:00
Microbial food safety of raw or minimally processed fresh produce is a significant challenge. The current sanitation processes are effective for inactivation of bacteria in wash water but have limited efficacy (<2 log/g reduction) for inactivation of microbes on the surfaces of fresh produce. This study demonstrates a novel concept to enhance effectiveness of chlorine using a particle-based...
Wed, 04/03/2019 - 06:50
Abstract An epidemiological investigation was conducted in an unvaccinated dairy farming enterprise in which three workers on one of the milking herds (Herd 1) were diagnosed with leptospirosis due to serovars Hardjo (H) (n = 2) and Pomona (P) (n = 1) between January and March 2015. Blood and urine samples were collected from milking cows in Herd 1 (N = 230) and Herd 2 (N = 400), rising one‐ (R1...
Wed, 04/03/2019 - 01:15
Available online 2 April 2019 Author(s): Xiao Cao, Lichao Zhao, Jingfeng Zhang, Xun Chen, Hui Xie, Yanlei Chang, Li WangAbstractAccurate and highly sensitive detection of viable but nonculturable (VBNC) Vibrio parahaemolyticus is crucial for preventing seafood-related outbreaks. The VBNC state may occur due to bacterial susceptibility to cold shock during food storage. Propidium monoazide (PMA)...
Wed, 04/03/2019 - 01:15
Available online 2 April 2019 Author(s): Amreeta Sarjit, Joshua T. Ravensdale, Ranil Coorey, Narelle Fegan, Gary A. DykesAbstractSalmonella is a foodborne bacterial pathogen associated with red meat production worldwide. Survival of, and cross contamination with, this pathogen at any stage from the farm to the processing facility may result in human disease. Cattle, goat and sheep are common...
Wed, 04/03/2019 - 01:15
Available online 1 April 2019 Author(s): Sang-Soon Kim, Jae-Ik Lee, Dong-Hyun KangAbstractSalsa is a liquid-solid food containing jalapeño and serrano peppers, which result in multistate outbreak in 2008. Storage temperature and sodium chloride (NaCl) concentration of salsa vary depending on the climate, season, and type of product. In this regard, effect of growth conditions, namely, low...
Wed, 04/03/2019 - 01:13
Available online 2 April 2019 Author(s): Tatiane Kuka Valente Gandra, Darla Volcan, Isabela Schneid Kroning, Naciele Marini, Antônio Costa de Oliveira, Caroline Peixoto Bastos, Wladimir Padilha da SilvaAbstractThe objective of this study was to compare the gene expression levels of the agr locus and prfA gene during adhesion and biofilm formation by four L. monocytogenes isolates (2 biofilm-...
Wed, 04/03/2019 - 01:13
Available online 1 April 2019 Author(s): Anastasia E. Lytou, Konstantinos Tzortzinis, Panagiotis N. Skandamis, George-John E. Nychas, Efstathios Z. PanagouAbstractThe aim of this work was to study the influence of lemon and vinegar marinades on Salmonella inoculated on chicken fillets and stored under different storage temperatures for nine days in the presence of indigenous microbiota. In...
Wed, 04/03/2019 - 00:00
Reliable detection of foodborne pathogen is of great importance in guaranteeing food safety. In the present work, based on the multifunctional properties of platinum-coated gold nanorods (AuNR@Pt), we developed a dual-mode aptasensor based on inner filter effect (IFE) and color change strategies and applied it for Vibrio parahaemolyticus (V. parahaemolyticus) detection. Under...
Tue, 04/02/2019 - 20:00
Brucellosis is a zoonotic disease caused by Brucella species. It has been estimated that more than 500,000 new cases of Brucellosis occur annually all around the world. Relapse of the disease is one of the most i...
Tue, 04/02/2019 - 11:02
Bacteria can be social and move in groups. Here, we show that Vibrio parahaemolyticus coordinates motility using the S signal, a cell‐cell signaling molecule that serves as a chemoattractant during swimming and swarming. Two methyl‐accepting chemoreceptors detect this autoinducer. One is necessary for S signal reception by liquid‐grown bacteria, while the other is important for chemotaxis by...
Mon, 04/01/2019 - 20:00
Aeromonas hydrophila is an opportunistic pathogen of poikilothermic and homoeothermic animals, including humans. In the present study, we described the role of Alanine racemase (alr-2) in the virulence of A. hydr...
Mon, 04/01/2019 - 12:01
Summary Early gut microbial colonization is important for postnatal metabolic and immune development. However, little is known about the effects of different feeding modes (suckling versus bottle‐feeding) or microbial sources on this process in farm animals. We found that suckled and bottle‐fed newborn lambs had their own distinct gut microbiota. Results from 16S rRNA gene sequencing and qPCR...
Mon, 04/01/2019 - 12:01
Summary Early gut microbial colonization is important for postnatal metabolic and immune development. However, little is known about the effects of different feeding modes (suckling versus bottle‐feeding) or microbial sources on this process in farm animals. We found that suckled and bottle‐fed newborn lambs had their own distinct gut microbiota. Results from 16S rRNA gene sequencing and qPCR...
Mon, 04/01/2019 - 11:50
The Gram-negative cell envelope is a remarkable structure with core components that include an inner membrane, an outer membrane, and a peptidoglycan layer in the periplasmic space between. Multiple molecular systems function to maintain integrity of this essential barrier between the interior of the cell and its surrounding environment. We show that a conserved DUF1849-family protein, EipB, is...
Mon, 04/01/2019 - 11:50
Typhoid fever is caused primarily by Salmonella enterica serovar Typhi (S. Typhi). Approximately 3-5% of individuals infected with S. Typhi become chronic carriers with the gallbladder (GB) as the site of persistence, as gallstones within the GB are a platform on which the bacteria form a biofilm. S. Typhi is a human-restricted pathogen; therefore, asymptomatic carriers represent a critical...
Mon, 04/01/2019 - 11:26
Abstract Viable but nonculturable (VBNC) Vibrio parahaemolyticus cannot be detected by the standard cultivation‐based methods. In this study, commonly used viability assessment methods were evaluated for the detection of Vibrio parahaemolyticus in a VBNC state. V. parahaemolyticus cells exposed to nutrient‐deficiency at cold temperature were used for epifluorescence microscopy with SYTO9 and...
Mon, 04/01/2019 - 05:44
Abstract The occurrence of E. coli O157 and Salmonella spp. in solid bovine manure was investigated through a multi‐county survey in California. Solid bovine manure samples (n=91) were collected from 13 dairy farms located in multiple counties in California between June 2016 and August 2017. To quantify pathogens, DNA was extracted from bacteria in manure samples. Afterward, the prevalence and...
Sun, 03/31/2019 - 01:12
Available online 29 March 2019 Author(s): Annabel L. Naditz, Monika Dzieciol, Martin Wagner, Stephan Schmitz-EsserAbstractListeria monocytogenes is a food-borne pathogen responsible for the disease listeriosis and is commonly isolated from food and food production facilities. Many L. monocytogenes strains contain plasmids, though the contributions of plasmids to survival in food production...
Sun, 03/31/2019 - 01:09
Available online 28 March 2019 Author(s): Raman Karthikeyan, Pratapa Gayathri, Paramasamy Gunasekaran, Medicharla V. Jagannadham, Jeyaprakash RajendhranAbstractMembrane vesicles (MVs) are produced by various Gram positive and Gram negative pathogenic bacteria and play an important role in virulence. In this study, the membrane vesicles (MVs) of L. monocytogenes were isolated from the culture...
Sat, 03/30/2019 - 01:15
Available online 28 March 2019 Author(s): Alva Smith, Jack Hearn, Clare Taylor, Nick Wheelhouse, Maciej Kaczmarek, Edwin Moorhouse, Ian SingletonAbstractListeria monocytogenes is sporadically detected on a range of ready to eat fresh produce lines, such as spinach and rocket, and is a threat to public health. However, little is known about the diversity of L. monocytogenes present on fresh...
Sat, 03/30/2019 - 01:15
Available online 28 March 2019 Author(s): Najeeb S. Al-zoreky, Abdulla Y. Al-TaherAbstractEssential oils extracted by hydro-distillation form date palm spathe (byproduct from date palm plants) were tested for their antibacterial activity against some food-borne pathogens. Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 29243, Salmonella enterica subsp. enterica serovar Enteritidis...
Sat, 03/30/2019 - 01:15
Available online 27 March 2019 Author(s): Gilberto Ordaz, José Ángel Merino-Mascorro, Santos García, Norma HerediaAbstractIn order to determine the microbial safety of produce, conventional fecal indicator bacteria (CFIB) such as Escherichia coli and Enterococcus are quantified as a standard practice. Bacteroidales are also fecal indicators mostly used for water samples; however, their use and...
Sat, 03/30/2019 - 01:15
Available online 28 March 2019 Author(s): Lin Wang, Xuetong Fan, Kimberly Sokorai, Joseph SitesAbstractGaseous ozone was evaluated for its effectiveness in reducing populations of Salmonella and native microorganisms on grape tomatoes and its impact on sensory and nutritional quality of the fruit. Grape tomatoes with Salmonella enterica serovar Typhimurium inoculated onto stem scar and smooth...
Sat, 03/30/2019 - 01:13
Anna-Maria Schmidt, Ulrike Escher, Soraya Mousavi, Manja Boehm, Steffen Backert, Stefan Bereswill, Markus M. Heimesaat
Sat, 03/30/2019 - 01:13
Available online 28 March 2019 Author(s): Chase E. Golden, Michael J. Rothrock, Abhinav MishraAbstractFoodborne pathogens such as Listeria spp. contain the ability to survive and multiply in poultry farming environments, which provides a route of contamination for poultry processing environments and final poultry products. An understanding of the effect of meteorological variables on the...
Sat, 03/30/2019 - 00:00
Toxins, Vol. 11, Pages 190: Nasal Colonization of Humans with Occupational Exposure to Raw Meat and to Raw Meat Products with Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus Toxins doi: 10.3390/toxins11040190 Authors: Christiane Cuny Franziska Layer Sonja Hansen Guido Werner Wolfgang Witte Livestock-associated methicillin-resistant Staphylococcus aureus (LA...
Fri, 03/29/2019 - 09:54
The purpose of this study is to establish a rapid and accurate method based on multiplex polymerase chain reaction combined with suspension array xTAG (Flexible Sequence‐tagged, xTAG) technology to simultaneously detect Salmonella Typhimurium, Brucella spp., Bacillus cereus and Shigella spp. in raw milk. Summary The purpose of this study is to establish a rapid and accurate method based on...
Fri, 03/29/2019 - 08:47
Abstract Aims Methicillin‐sensitive and methicillin‐resistant Staphylococcus aureus (MSSA and MRSA, respectively) in the nostrils of dogs and workers at an animal shelter were cultured. Staphylococcal toxin genes were analyzed to identify potential health concerns. Methods and Results Samples were obtained from 441 dogs and 9 workers. The respective isolation rates of S. aureus and MRSA were...
Fri, 03/29/2019 - 01:11
Publication date: Available online 27 March 2019Source: LWTAuthor(s): Daniel Kuhn, Rafaela Ziem, Talita Scheibel, Bárbara Buhl, Gabriela Vettorello, Leandra Andressa Pacheco, Daiane Heidrich, Carla Kauffmann, Elisete M. de Freitas, Eduardo M. Ethur, Lucélia HoehneAbstractCampomanesia aurea O. Berg is a native plant of the biome Pampa, which belongs to the family Myrtaceae. The present study aimed...
Fri, 03/29/2019 - 01:11
Publication date: Available online 26 March 2019Source: LWTAuthor(s): Edson A. Rios, Jesús Santos, Isidro García-Meniño, Saskia C. Flament-Simon, Jorge Blanco, María-Luisa García-López, Andrés Otero, Jose M. Rodríguez-CallejaAbstractThis study was carried out to determine the occurrence and characteristics of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC)...
Thu, 03/28/2019 - 11:00
Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children from developing countries and presents high genetic variability. We aimed to characterize the EPEC virulence-related gene (VRG) distribution and copathogens associated with diarrhea and nutrition-related outcomes in children from the low-income Brazilian semiarid region. A cross-sectional case-control study of...
Thu, 03/28/2019 - 11:00
Azithromycin (AZM) has been recommended by the American Academy of Pediatrics for the treatment of shigellosis in children. In this study, 502 Shigella species isolated between 2004 and 2014 were tested for AZM epidemiological cutoff values (ECV) by disk diffusion. AZM MICs and the presence of the macrolide resistance genes [erm(A), erm(B), erm(C), ere(A), ere(B), mph(A), mph(B), mph(D), mef(A),...
Thu, 03/28/2019 - 11:00
Shigella spp. are a leading cause of human diarrheal disease worldwide, with Shigella flexneri being the most frequently isolated species in developing countries. This serogroup is presently classified into 19 serotypes worldwide. We report here a multicenter validation of a multiplex-PCR-based strategy previously developed by Q. Sun, R. Lan, Y. Wang, A. Zhao, et al. (J Clin Microbiol 49:3766...
Thu, 03/28/2019 - 09:53
Abstract This study aimed to analyse the frequency of genes encoding virulence factors and to characterize resistance profiles in Staphylococcus aureus isolated from raw milk. Forty‐seven and nine S. aureus isolates were recovered from 150 and 100 raw bovine and ovine milk samples, respectively, in Tunisia. The majority of isolates was resistant to penicillin, and no methicillin‐resistant S....
Thu, 03/28/2019 - 01:15
Beilei Ge, Kelly J. Domesle, Qianru Yang, Thomas S. Hammack, Shizhen S. Wang, Xiaohong Deng, Lijun Hu, Guodong Zhang, Yuan Hu, Xiaokuang Lai, Kyson X. Chou, Jan Ryan Dollete, Kirsten A. Hirneisen, Sammie P. La, Richelle S. Richter, Diyo R. Rai, Azadeh A. Yousefvand, Paul K. Park, Cindy H. Wu, Tameji Eames, David Kiang, Ju Sheng, Dancia Wu, Lori Hahn, Lisa Ledger, Cynthia Logie, Qiu You, Durda...
Thu, 03/28/2019 - 01:15
Ana María Quesille-Villalobos, Angel Parra, Felipe Maza, Paola Navarrete, Mauricio González, Mauricio Latorre, Magaly Toro, Angélica Reyes-Jara
Thu, 03/28/2019 - 01:13
Publication date: 15 August 2019 , Volume 289Author(s): Deyun He, Zhengzong Wu, Bo Cui, Enbo Xu, Zhengyu JinAbstractCampylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis. Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS)...
Thu, 03/28/2019 - 01:13
Available online 27 March 2019 Author(s): Lihui Zhang, James G. Lyng, Ruzhen Xu, Shuang Zhang, Xu Zhou, Shaojin WangAbstractRadio frequency (RF) treatment is considered as a potential method for eliminating food-borne pathogens from low moisture foods. In this study, a treatment protocol for pasteurizing in-shell walnuts (with 15.01% w.b. of moisture) was developed using a 6 kW, 27.12 MHz RF oven...
Wed, 03/27/2019 - 08:00
Abstract Heracleum persicum is a traditional medicinal plant with several pharmaceutical and food applications. There is no well‐documented information regarding the safety profile of this plant. The aim of this study was to evaluate the genotoxic, mutagenic, and cytotoxic potential of ethanolic extract of H. persicum (EEHP). The EEHP induced DNA damage at concentrations higher than 0.25 mg/mL....
Wed, 03/27/2019 - 08:00
Abstract Heracleum persicum is a traditional medicinal plant with several pharmaceutical and food applications. There is no well‐documented information regarding the safety profile of this plant. The aim of this study was to evaluate the genotoxic, mutagenic, and cytotoxic potential of ethanolic extract of H. persicum (EEHP). The EEHP induced DNA damage at concentrations higher than 0.25 mg/mL....
Wed, 03/27/2019 - 01:14
Publication date: Available online 25 March 2019Source: LWTAuthor(s): R. Raybaudi-Massilia, J. Mosqueda-Melgar, Y. Rosales-Oballos, R. Citti de Petricone, N.N. Frágenas, A. Zambrano-Durán, K. Sayago, M. Lara, G. UrbinaAbstractIn this research, the effect of partial replacement of NaCl by Soda-Lo® salt microspheres (25% (W/W), 30% (W/W), and 50% (W/W)) on the sensory and microbiological properties...

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