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Bacterial pathogens

Thu, 03/21/2019 - 14:51
Abstract Aims In this present study, the utility of a newly developed Loop mediated isothermal amplification (LAMP) and real‐time PCR assays designed to amplify the virB gene region of B. melitensis were evaluated from human clinical specimens. Methods and Results 54 culture confirmed cases of brucellosis and 54 culture negative but clinically suspected cases of brucellosis were included in...
Thu, 03/21/2019 - 01:18
Available online 19 March 2019 Author(s): Matthew Aijuka, Elna M. BuysAbstractDiarrheagenic Escherichia coli (DEC) is a leading cause of foodborne illness associated with intestinal disease. While known over the years that contamination of food sources occurs via the oral faecal-route, the mechanisms underlying its persistence within the open environments including the food chain remains...
Thu, 03/21/2019 - 01:18
Available online 19 March 2019 Author(s): Aline Crucello, Marianna M. Furtado, Monyca D.R. Chaves, Anderson S. Sant’AnaAbstractSalmonella enterica serotypes have been reported as the agent of various outbreaks occurred after the consumption of low water activity (aw) foods. When the pathogen encounters harsh conditions, several regulatory networks are activated through dynamic differential gene...
Thu, 03/21/2019 - 01:18
Available online 19 March 2019 Author(s): Serajus Salaheen, Seon Woo Kim, Jeffrey S. Karns, Ernest Hovingh, Bradd J. Haley, Jo Ann S. Van KesselAbstractEscherichia coli O157:H7 (O157:H7) is a major zoonotic pathogen responsible for severe and sometimes fatal infections in humans. Cattle are the primary reservoir of O157:H7, although other animal species are also known carriers. Fecal shedding of...
Thu, 03/21/2019 - 01:18
Available online 20 March 2019 Author(s): Tatsuya TominagaAbstractColiform bacteria in foods are enumerated at food processing plants and are used as sanitary and quality indicators. To detect coliform bacteria rapidly, seven Lateral Flow Test Strips (LFTSs) that can detect the genera Aeromonas, Citrobacter, Enterobacter, Hafnia, Klebsiella/Raoultella, Pantoea and Serratia were developed. For 55...
Wed, 03/20/2019 - 01:13
Md. Ashrafudoulla, Md. Furkanur Rahaman Mizan, Heedae Park, Kye-Hwan Byun, Nari Lee, Si Hong Park, Sang-Do Ha
Wed, 03/20/2019 - 01:12
Available online 18 March 2019 Author(s): Victoria E. Watson, Megan E. Jacob, José M. Bruno-Bárcena, Sophia Amirsultan, Stephen H. Stauffer, Victoria O. Píqueras, Rafael Frias, Jody L. GookinAbstractTypical enteropathogenic E. coli (tEPEC) carries the highest hazard of death in children with diarrhea and atypical EPEC (aEPEC) was recently identified as significantly associated with diarrheal...
Wed, 03/20/2019 - 01:12
Available online 18 March 2019 Author(s): Seyedeh-Maryam Hasheminya, Reza Rezaei Mokarram, Babak Ghanbarzadeh, Hamed Hamishekar, Hossein Samadi Kafil, Jalal DehghannyaAbstractKefiran-carboxymethyl cellulose biocomposite films incorporated with Satureja Khuzestanica essential oil were developed and characterized. Results indicated that increase in the concentration of the essential oil increased...
Wed, 03/20/2019 - 01:12
Available online 18 March 2019 Author(s): Shravani Tadepalli, David F. Bridges, Ryan Anderson, Rong Zhang, Vivian C.H. WuAbstractTo increase the safety of fruits and vegetables, including blueberries, this study developed an efficient and practical treatment strategy that implements multiple antimicrobial wash “hurdles” using industrial realistic treatment times in combination with freezing (at −...
Tue, 03/19/2019 - 20:00
Bacteriocins produced by lactic acid bacteria (LAB) can be considered as viable alternatives for food safety and quality, once these peptides present antimicrobial activity against foodborne pathogens and spoi...
Tue, 03/19/2019 - 20:00
Species-specific differences in tolerance to infection are exemplified by the high susceptibility of humans to enterohemorrhagic Escherichia coli (EHEC) infection, whereas mice are relatively resistant to this pa...
Tue, 03/19/2019 - 15:00
Abstract Salmonella Kentucky is among the most frequently isolated S. enterica serovars from food animals in the United States. Recent research on isolates recovered from these animals suggests there may be geographic and host specificity signatures associated with S. Kentucky strains. However, the sources and genomic features of human clinical S. Kentucky isolated in the United States remain...
Tue, 03/19/2019 - 10:21
Significance and Impact of the Study: PCR screening revealed the significant presence of the genetic markers of Shiga toxin‐producing Escherichia coli (STEC) (stx + /stx + eae + ) on the surfaces of sheep carcasses. Citrobacter braakii (stx 1 + eae + ) was isolated for the first time in this study. The risk of foodborne diseases due to STEC must be taken into account...
Tue, 03/19/2019 - 09:47
Abstract Aims This study conducted bacterial community, virulence and antibiogram profiling inside the hindgut and skin of freshly caught hilsa fish and those sold at markets. Methods and Results The results of 16S rRNA‐based high‐throughput sequencing showed a higher number of bacterial genera in marketed fish samples than in fresh fish samples. The total operational taxonomic units (OTU),...
Tue, 03/19/2019 - 01:13
Available online 18 March 2019 Author(s): R.A.H. Timmermans, H.C. Mastwijk, L.B.J.M. Berendsen, A.L. Nederhoff, A.M. Matser, M.A.J.S. Van Boekel, M.N. Nierop GrootAbstractModerate intensity Pulsed Electric Fields (PEF) was studied for microbial inactivation as an alternative to high intensity PEF or to classical thermal pasteurization. The process is characterized by the application of electric...
Tue, 03/19/2019 - 01:13
Available online 18 March 2019 Author(s): Cristina Rodríguez-Melcón, Félix Riesco-Peláez, Camino García-Fernández, Carlos Alonso-Calleja, Rosa CapitaAbstractThe susceptibility of four L. monocytogenes isolates from pork to sodium hypochlorite (SHY) and benzalkonium chloride (BZK) was tested. Minimum inhibitory concentration (MIC) values of 3,500 ppm (SHY), or between 3 ppm and 13 ppm (BZK), were...
Tue, 03/19/2019 - 01:13
Available online 18 March 2019 Author(s): Julia Mougin, Stéphanie Copin, Daline Bojolly, Virginie Raguenet, Annick Robert-Pillot, Marie-Laure Quilici, Graziella Midelet-Bourdin, Thierry Grard, Maryse Bonnin-JusserandAbstractA single strain of Vibrio parahaemolyticus and a single stain of Vibrio cholerae were isolated from shrimps sourced from seafood processing plants. The isolated strains were...
Mon, 03/18/2019 - 13:04
Abstract We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests...
Mon, 03/18/2019 - 13:04
Abstract We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests...
Mon, 03/18/2019 - 11:18
Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be post-translationally modified for full functionality; in Enterobacteria this is accomplished by two enzymes, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a conserved lysine position. Mutations to efp or its modifying enzymes produce...
Mon, 03/18/2019 - 11:00
Summary Type VI secretion systems (T6SSs) are widespread, tightly regulated, protein delivery apparatuses used by Gram‐negative bacteria to outcompete their neighbors. The pathogen, Vibrio parahaemolyticus, encodes two T6SSs. These T6SSs are differentially regulated by external conditions. T6SS1, an antibacterial system predominantly found in pathogenic isolates, requires warm marine‐like...
Mon, 03/18/2019 - 01:14
Publication date: Available online 16 March 2019Source: Author(s): Heba A. Ahmed, Asmaa B.M.B. Tahoun, Rasha M.M. Abou Elez, Marwa I. Abd El-Hamid, Samah S. Abd EllatifAbstractYersinia enterocolitica was isolated from raw milk and dairy products from 10% of examined samples. The highest isolation rate was 22%, from raw milk, followed by 12%, 4% and 2% from fermented milk (Rayeb), pasteurised milk...
Sun, 03/17/2019 - 01:13
Publication date: Available online 15 March 2019Source: LWTAuthor(s): Abdus Sobhan, Jinyoung Lee, Mi-Kyung Park, Jun-Hyun OhAbstractThe goal of this research was to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor method and to employ it to a Kimchi product for Yersinia enterocolitica detection. A SWCNT-based biosensor was fabricated with SWCNTs immobilized with anti-Yersinia...
Sun, 03/17/2019 - 01:12
Available online 16 March 2019 Author(s): Roberta T. Melo, Ana Laura Grazziotin, Edson C.V. Júnior, Renata R. Prado, Eliane P. Mendonça, Guilherme P. Monteiro, Phelipe A.B.M. Peres, Daise A. RossiAbstractCampylobacter jejuni is the most common pathogen associated with foodborne diseases. Persistent presence of this pathogen contaminating the environment in slaughterhouses and chicken products...
Sun, 03/17/2019 - 01:12
Available online 15 March 2019 Author(s): Lucilla Iacumin, Giuseppe ComiAbstractThe aim of this study was to determine the microbial quality of mung bean sprouts produced in Italy. The presence of pathogenic microorganisms (Shiga toxin-producing Escherichia coli (STEC), Salmonella spp. and Listeria monocytogenes), total coliforms, and total viable counts (TVCs) were determined. The study covered...
Sat, 03/16/2019 - 01:14
Lun Zhang, Shuiqing Gui, Zhaobo Liang, Along Liu, Zhaoxia Chen, Yanan Tang, Mingzhu Xiao, Fujiang Chu, Wenbin Liu, Xiaobao Jin, Jiayong Zhu, Xuemei Lu
Sat, 03/16/2019 - 01:14
Available online 14 March 2019 Author(s): Gang Zhang, Kaiwen Sun, Guomin Ai, Jianjuan Li, Na Tang, Yuqin Song, Chao Wang, Jie FengAbstractIntrinsic resistance of bacteria to antibiotics plays an increasingly significant role in antibiotic resistance. However, the breadth of intrinsic resistance has not been fully elucidated. Here we identified a novel class of chloramphenicol acetyltransferase (...
Sat, 03/16/2019 - 01:12
Available online 14 March 2019 Author(s): Catherine Ngaywa, Gabriel O. Aboge, George Obiero, Isaac Omwenga, Nicholas Ngwili, George Wamwere, Martin Wainaina, Bernard BettAbstractAntimicrobial agents are used widely in veterinary and human medicine worldwide. However, their use has suffered a major setback globally due to the emergence of antimicrobial resistance (AMR) in a wide range of...
Fri, 03/15/2019 - 17:00
Antoine Dujardin, Peter De Wolf, Frank Lafont, Vincent Dupres In the last 20 years, atomic force microscopy (AFM) has emerged as a ubiquitous technique in biological research, allowing the analysis of biological samples under near-physiological conditions from single molecules to living cells. Despite its growing use, the low process throughput remains a major drawback. Here, we propose a...
Fri, 03/15/2019 - 17:00
Diah Ayuningrum, Yang Liu, Riyanti, Mada T. Sibero, Rhesi Kristiana, Meezan A. Asagabaldan, Zerlina G. Wuisan, Agus Trianto, Ocky Karna Radjasa, Agus Sabdono, Till F. Schäberle Tunicates (Ascidians, sea squirts) are marine protochordates, which live sedentary or sessile in colonial or solitary forms. These invertebrates have to protect themselves against predators and invaders. A most successful...
Fri, 03/15/2019 - 15:30
Vibrio cholerae is an aquatic bacterium with the potential to infect humans. This study focuses on the DNA replication and cell division of this bi‐chromosomal bacterium during starvation stress. Surprisingly, cells temporarily restart their DNA replication after a first shut‐down period before finally reaching a state with fully replicated single chromosome sets. Abstract Vibrio cholerae is...
Fri, 03/15/2019 - 01:14
Available online 13 March 2019 Author(s): Yunyun Li, Jinquan Wang, Yuchen Li, Haiqin Wu, Shiyi Zhao, Qinghua YuAbstractPigs suffer enteritis induced by pathogenic bacteria infection and toxins in the moldy feed, which cause intestinal epithelial damage and diarrhea through the whole breeding cycle. Interleukin-22 (IL-22) plays a critical role in maintaining intestinal mucosal barrier function...
Fri, 03/15/2019 - 01:12
Available online 13 March 2019 Author(s): Nydia Muñoz, Chandrashekhar R. Sonar, Kanishka Bhunia, Juming Tang, Gustavo V. Barbosa-Cánovas, Shyam S. SablaniAbstractThis study explored the antagonistic activity of Lactobacillus rhamnosus GG (LGG) (Culturelle®), a commercial probiotic supplement, on foodborne pathogens and its heat sensitivity in soup products. Staphylococcus aureus, Escherichia coli...
Fri, 03/15/2019 - 01:12
Available online 13 March 2019 Author(s): J. McLauchlin, H. Aird, N. Andrews, M. Chattaway, E. de Pinna, N. Elviss, F. Jørgensen, L. Larkin, C. WillisAbstractFresh betel leaves (Piper betle L.), imported into the UK are a traditional ready-to-eat food consumed by Asian populations. We report here the consolidation of routinely collected data to model the public health risks from consumption of...
Fri, 03/15/2019 - 01:12
Available online 13 March 2019 Author(s): F.F. Parlapani, S. Michailidou, D.A. Anagnostopoulos, S. Koromilas, K. Kios, K. Pasentsis, F. Psomopoulos, A. Argiriou, S.A. Haroutounian, I.S. BoziarisAbstractBacterial communities composition using 16S Next Generation Sequencing (NGS) and Volatile Organic Compounds (VOCs) profile of whole blue crabs (Callinectes sapidus) stored at 4 and 10 °C (proper...
Fri, 03/15/2019 - 01:12
Available online 13 March 2019 Author(s): Lakshmikantha H. Channaiah, Minto Michael, Jennifer C. Acuff, Randall K. Phebus, Harshavardhan Thippareddi, George MillikenABSTRACTThis study was conducted to validate a simulated commercial whole wheat multigrain bread baking process at 375°F (190.6°C) oven temperature for 35 min to inactivate Salmonella, and to determine the thermal inactivation...
Thu, 03/14/2019 - 01:15
Publication date: Available online 13 March 2019Source: LWTAuthor(s): Mohammad Shakeri, Seyed Hadi Razavi, Shahryar ShakeriAbstractCarvacrol (Car) and astaxanthin (Asta) are widely used in the food industry as antimicrobial and antioxidant agents. This study investigated simultaneous entrapment of them in beeswax solid lipid nanoparticles (BW.SLNs) in order to increase their biological activities...
Thu, 03/14/2019 - 01:15
Publication date: Available online 13 March 2019Source: LWTAuthor(s): Flávia Souza Prestes, Astrid Caroline Muniz da Silva, André Aquino Mariano Pereira, Maristela da Silva do NascimentoAbstractThis study evaluated the efficiency of the dry and oil roasting processes in the inactivation of a pool of five serotypes of Salmonella isolated from the peanut supply chain (Miami, Muenster, Yoruba,...
Thu, 03/14/2019 - 01:15
Publication date: Available online 12 March 2019Source: LWTAuthor(s): Pawinee Inmanee, Pitiya Kamonpatana, Tantawan PirakAbstractListeria monocytogenes contamination of processed sausage occurs primarily after cooking and during packaging, causing significant annual economic loss throughout the industry. Ohmic heating of vacuum-packed sausages was proposed for effective post packaging...
Thu, 03/14/2019 - 01:13
Mohammad Aminul Islam, Sahana Parveen, Mahdia Rahman, Mohsina Huq, Ashikun Nabi, Zahed Uddin Mahmood Khan, Niyaz Ahmed, Jaap A. Wagenaar
Thu, 03/14/2019 - 01:13
Available online 12 March 2019 Author(s): Svenja Mamerow, Robert Scheffter, Susanne Röhrs, Olga Stech, Ulrike Blohm, Theresa Schwaiger, Charlotte Schröder, Reiner Ulrich, Jan Schinköthe, Martin Beer, Thomas C. Mettenleiter, Jürgen StechAbstractInfluenza A viruses (IAV) have caused seasonal epidemics and severe pandemics in humans. Novel pandemic strains as in 2009 may emerge from pigs, serving as...
Thu, 03/14/2019 - 01:13
Available online 11 March 2019 Author(s): Yao Jin, Dengyong Li, Mei Ai, Qiuxiang Tang, Jun Huang, Xiaofei Ding, Chongde Wu, Rongqing ZhouAbstractMicrobial community diversity and volatile profiles of 14 Jiang-flavor liquor Daqu (JFLQ) samples were investigated by Illumina MiSeq platform and Head Space-solid Phase Microextraction Gas Chromatograph Mass Spectrometer (HS-SPME-GC-MS). Correlations...
Wed, 03/13/2019 - 17:00
Reza Farahani Khaltabadi, Nader Shahrokhi, Mina Ebrahimi-Rad, Parastoo Ehsani Salmonella Typhimurium, a zoonotic pathogen, is regarded as a major health and economic concern worldwide. Recently, monophasic variants of this serovar have been significantly associated with human gastroenteritis outbreaks globally, making its accurate identification essential for epidemiological and control purposes...
Wed, 03/13/2019 - 11:47
Summary Blackleg and soft rot are devastating diseases on potato stem and tuber caused by Pectobacterium and Dickeya pectinolytic enterobacteria. In European potato cultures, D. dianthicola and D. solani species successively emerged in the past decades. Ecological traits associated to their settlement remain elusive, especially in the case of the recent invader D. solani. In this work, we...
Wed, 03/13/2019 - 11:47
Summary Vibrio parahaemolyticus, the leading cause of seafood‐associated gastroenteritis worldwide, requires the two type‐III secretion systems (T3SS1 and T3SS2) and a thermostable direct hemolysin (encoded by tdh1 and tdh2) for full virulence. The tdh genes and the T3SS2 gene cluster constitute an 80 kb pathogenicity island known as Vp‐PAI located on the chromosome II. Expression of T3SS1 and...
Wed, 03/13/2019 - 11:47
Summary Blackleg and soft rot are devastating diseases on potato stem and tuber caused by Pectobacterium and Dickeya pectinolytic enterobacteria. In European potato cultures, D. dianthicola and D. solani species successively emerged in the past decades. Ecological traits associated to their settlement remain elusive, especially in the case of the recent invader D. solani. In this work, we...
Wed, 03/13/2019 - 11:47
Summary Vibrio parahaemolyticus, the leading cause of seafood‐associated gastroenteritis worldwide, requires the two type‐III secretion systems (T3SS1 and T3SS2) and a thermostable direct hemolysin (encoded by tdh1 and tdh2) for full virulence. The tdh genes and the T3SS2 gene cluster constitute an 80 kb pathogenicity island known as Vp‐PAI located on the chromosome II. Expression of T3SS1 and...
Wed, 03/13/2019 - 01:12
Available online 11 March 2019 Author(s): Hsieh-Chin Tsai, Michael H. Taylor, Xia Song, Lina Sheng, Juming Tang, Mei-Jun ZhuAbstractListeria monocytogenes can survive in dry conditions for long periods. Despite an increasing research studying Salmonella inactivation in low-moisture foods, there is a general lack of knowledge related to L. monocytogenes inactivation in low-moisture foods during...
Wed, 03/13/2019 - 01:12
Available online 12 March 2019 Author(s): Katia Rouzeau-Szynalski, Caroline Barretto, Coralie Fournier, Deborah Moine, Johan Gimonet, Leen BaertAbstractIn 2013, during a routine laboratory analysis performed on food samples, one finished product from a European factory was tested positive for Salmonella Hadar. At the same period, one environmental isolate in the same laboratory was serotyped...

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