You are here

Bacterial pathogens

Thu, 05/23/2019 - 12:34
Abstract Listeria monocytogenes (L. monocytogenes) is a major concern in human food safety. Here, the development of a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) method, targeting the hly A gene of L. monocytogenes, to rapidly detect this important pathogen in food is described. The RT‐LAMP conditions were optimized and the following parameters established: 60 min,...
Thu, 05/23/2019 - 12:34
Abstract Listeria monocytogenes (L. monocytogenes) is a major concern in human food safety. Here, the development of a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) method, targeting the hly A gene of L. monocytogenes, to rapidly detect this important pathogen in food is described. The RT‐LAMP conditions were optimized and the following parameters established: 60 min,...
Thu, 05/23/2019 - 09:52
Abstract The general stress response (GSR) allows many bacterial species to react to myriad different stressors. In Alphaproteobacteria, this signaling pathway proceeds through the partner‐switching PhyR‐EcfG sigma‐factor mechanism and is involved in multiple life processes, including virulence in Brucella abortus. To date, details of the alphaproteobacterial GSR signaling pathway have been...
Thu, 05/23/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 1022-1027, June 2019.
Thu, 05/23/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 1022-1027, June 2019.
Thu, 05/23/2019 - 01:12
Available online 20 May 2019 Author(s): Felipe Miceli de Farias, Janaína dos Santos Nascimento, Olinda Cabral da Silva Santos, Maria do Carmo de Freire BastosAbstractReducing salt content in foods such as cheeses, while limiting the growth of spoilage microorganisms and foodborne pathogens, is a difficult challenge. One method that may prove useful is use of staphylococcins, which are...
Thu, 05/23/2019 - 00:00
Toxins, Vol. 11, Pages 291: Study on the Growth and Enterotoxin Production by Staphylococcus aureus in Canned Meat before Retorting Toxins doi: 10.3390/toxins11050291 Authors: Luca Grispoldi Paul Alexanderu Popescu Musafiri Karama Vito Gullo Giusi Poerio Elena Borgogni Paolo Torlai Giuseppina Chianese Anna Giovanna Fermani Paola Sechi Beniamino Cenci-Goga Possible...
Wed, 05/22/2019 - 17:00
Katharina Loreck, Sylvia Mitrenga, Diana Meemken, Regina Heinze, Annett Reissig, Elke Mueller, Ralf Ehricht, Claudia Engemann, Matthias Greiner In order to monitor the occurrence of zoonotic agents in pig herds as well as to improve herd health management, the development of new cost-effective diagnostic methods for pigs is necessary. In this study, a protein microarray-based assay for the...
Wed, 05/22/2019 - 15:00
Abstract Susceptibility profiles were determined for 111 Campylobacter coli strains obtained in 1998 to 1999 and 2015 from market age pigs and pork chops against 22 disinfectants and 9 antimicrobials. Resistance to tetracycline (TET) was observed in 44.4% of 1998 to 1999 strains, and the antibiotic resistance profile was TET. But strains obtained in 2015 from swine and retail pork chops had 75%...
Wed, 05/22/2019 - 13:00
Abstract Listeria monocytogenes is a major foodborne pathogen that may contaminate liquid egg yolk (LEY). A background microbiota, purified and identified as Enterococcus faecium with a 99.0% probability, was found in pasteurized unsalted LEY. This study was conducted to investigate the thermal resistance of L. monocytogenes and the background microbiota in unsalted and 10% salted LEY at...
Wed, 05/22/2019 - 13:00
Abstract Listeria monocytogenes is a major foodborne pathogen that may contaminate liquid egg yolk (LEY). A background microbiota, purified and identified as Enterococcus faecium with a 99.0% probability, was found in pasteurized unsalted LEY. This study was conducted to investigate the thermal resistance of L. monocytogenes and the background microbiota in unsalted and 10% salted LEY at...
Wed, 05/22/2019 - 12:03
Abstract Brazil is home to a wide variety of flora, including several lesser known species, such as araçá that were processed in this study using two green technologies consecutively: supercritical fluid extraction (SFE) for nonpolar fraction and pressurized liquid extraction. For polar fraction, the experiments followed a central composite design involving ethanol as solvent, with temperature...
Wed, 05/22/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 997-1006, June 2019.
Wed, 05/22/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 997-1006, June 2019.
Wed, 05/22/2019 - 00:00
Some Lactobacillus strains may contribute to the health of the host when administered in adequate concentrations, demonstrating their probiotic potential. In contrast, Listeria monocytogenes is a foodborne pathogen that can cause enteropathy, meningoencephalitis, abortion, and septicemia. The aim of this survey was to evaluate the in vitro and in vivo probiotic potential of Lactobacillus...
Tue, 05/21/2019 - 17:00
Hisaya K. Ono, Shouhei Hirose, Kouji Narita, Makoto Sugiyama, Krisana Asano, Dong-Liang Hu, Akio Nakane Staphylococcal enterotoxins (SEs) produced Staphylococcus aureus are known as causative agents of emetic food poisoning. We previously demonstrated that SEA binds with submucosal mast cells and evokes mast cell degranulation in a small emetic house musk shrew model. Notably, primates have been...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Tue, 05/21/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 980-987, June 2019.
Tue, 05/21/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 980-987, June 2019.
Tue, 05/21/2019 - 01:13
Publication date: Available online 20 May 2019Source: LWTAuthor(s): Debasish Kumar Dey, Bon Gyo Koo, Chanchal Sharma, Sun Chul KangAbstractFoodborne pathogenic bacteria like, Escherichia coli O157:H7, Bacillus cereus and Salmonella typhimurium are the biggest threat to food industries. Their growth in the environment and transmission of deadly diseases has become a challenge. The present study...
Tue, 05/21/2019 - 01:12
Available online 18 May 2019 Author(s): Jesús F. Barandika, Raquel Alvarez-Alonso, Isabel Jado, Ana Hurtado, Ana L. García-PérezAbstractQ fever is a bacterial zoonosis caused by Coxiella burnetii whose main reservoir are small ruminants. Infected animals shed the bacteria into the environment through the products of abortion as well as through feces, urine, and milk. Susceptible people are mainly...
Mon, 05/20/2019 - 17:00
Ignacio Santecchia, Frédérique Vernel-Pauillac, Orhan Rasid, Jessica Quintin, Maria Gomes-Solecki, Ivo G. Boneca, Catherine Werts Leptospira interrogans are pathogenic spirochetes responsible for leptospirosis, a worldwide reemerging zoonosis. Many Leptospira serovars have been described, and prophylaxis using inactivated bacteria provides only short-term serovar-specific protection. Therefore,...
Mon, 05/20/2019 - 08:28
This study aimed to isolate bacteriocin‐like inhibitory substance (BLIS) producing strains from raw buffalo milk and characterize their probiotic and antimicrobial potential. The BT‐11 isolate was identified through 16S rDNA gene sequencing and recA gene amplification as Lactobacillus paraplantarum. The BT‐11 strain survived in low pH (2, 3) and bile (0.5, 1, and 2%) for 3 hr of incubation. The...
Mon, 05/20/2019 - 00:00
Toxins, Vol. 11, Pages 281: Binding to The Target Cell Surface Is The Crucial Step in Pore Formation of Hemolysin BL from Bacillus cereus Toxins doi: 10.3390/toxins11050281 Authors: Nadja Jessberger Richard Dietrich Stefanie Schwemmer Franziska Tausch Valerie Schwenk Andrea Didier Erwin Märtlbauer A major virulence factor involved in Bacillus cereus food poisoning is the three...
Sat, 05/18/2019 - 01:12
Publication date: 1 October 2019 , Volume 294Author(s): Erwei Wang, Yaqin Li, Bibole Lubamba Maguy, Zaixiang Lou, Hongxin Wang, Wanqing Zhao, Xiaohua ChenAbstractSeparation and enrichment of phenolic components from Citrus medica L. var. sarcodactylis were performed to improve the antibiofilm and antibacterial activity/efficiency of the fractions for the first time. Through separation of the...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...
Fri, 05/17/2019 - 01:14
Publication date: Available online 15 May 2019Source: LWTAuthor(s): Mehran Moradi, Karim Mardani, Hossein TajikAbstractAnti-listeria and functional characteristics of postbiotics (metabolic byproducts of probiotics) of Lactobacillus acidophilus LA5 and L. casei 431 and L. salivarius were investigated in vitro, and in ground meat and whole milk. Antibacterial activity of all examined postbiotics...
Fri, 05/17/2019 - 01:12
Publication date: 1 October 2019 , Volume 294Author(s): Hongwei Zheng, Feng Han, Hong Lin, Limin Cao, Tushar Ramesh Pavase, Jianxin SuiAbstractDetection of pathogenic bacteria by polymerase chain reaction (PCR) is progressively emerging, although it is still hindered by a complex matrix, long-term bacterial enrichment and low bacterial abundance. Here, we report a novel material based on boronate...
Fri, 05/17/2019 - 01:12
Available online 15 May 2019 Author(s): Simone Buess, Katrin Zurfluh, Roger Stephan, Claudia GuldimannAbstractWe performed a quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir. At each of six selected process steps (after plucking, cloaca excision, evisceration, washing, water spray chiller and dry air chiller), 103 carcasses from 50 flocks were...
Fri, 05/17/2019 - 01:12
Available online 14 May 2019 Author(s): Simone Mancini, Gisella Paci, Valentina Ciardelli, Barbara Turchi, Francesca Pedonese, Filippo FratiniAbstractToday, edible insects represent a hot topic as an emerging and eco-friendly source of protein. The mealworm (Tenebrio molitor L.) is among the most employed insects for human consumption and feed purposes. So far Listeria monocytogenes, have never...
Thu, 05/16/2019 - 20:00
Campylobacter jejuni infections constitute serious threats to human health with increasing prevalences worldwide. Our knowledge regarding the molecular mechanisms underlying host–pathogen interactions is still li...
Thu, 05/16/2019 - 20:00
A total of forty-four lactic acid bacteria (LAB) strains originally isolated from cattle feces and different food sources were screened for their potential probiotic features. The antimicrobial activity of all isolates was tested by well-diffusion assay and competitive exclusion on broth against Salmonella Montevideo, Escherichia coli O157:H7 and Listeria monocytogenes strain N1-002. Thirty-eight...
Thu, 05/16/2019 - 17:00
Luke A. Schwerdtfeger, Nora Jean Nealon, Elizabeth P. Ryan, Stuart A. Tobet Background Human intestines contain a heterogeneous collection of cells that include immune, neural and epithelial elements interacting in a highly complex physiology that is challenging to maintain ex vivo. There is an extreme oxygen gradient across the intestinal wall due in part to microbiota in the lumen and close to...
Thu, 05/16/2019 - 17:00
Vincenzo Motta, Diana Luise, Paolo Bosi, Paolo Trevisi The host-microbiota interplay is recognized as a key factor for the homeostatic maintenance in animals. In pigs, the weaning transition represents a drastic changes event leading to high risk of gut dysbiosis, which in most cases results in economic losses for swine industry. The blood type antigens expressed on mucosal surfaces can act as...
Thu, 05/16/2019 - 11:01
Contamination of fresh produce with pathogenic Escherichia coli, including Shiga-toxigenic E. coli (STEC), represents a serious risk to human health. Colonization is governed by multiple bacterial and plant factors that can impact the probability and suitability of bacterial growth. Thus, we aimed to determine whether the growth potential of STEC for plants associated with foodborne outbreaks (...
Thu, 05/16/2019 - 11:01
Campylobacter is a major foodborne pathogen in humans and a significant cause of abortion in sheep. Although ruminants are increasingly recognized as important reservoirs for Campylobacter species, limited information is available about the molecular epidemiology and antimicrobial resistance (AMR) profiles of sheep Campylobacter. Here, we describe a two-trial study that examined Campylobacter...
Thu, 05/16/2019 - 11:01
Microcin PDI (MccPDI), a class IIa microcin that is produced by Escherichia coli strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic E. coli serotypes O157:H7 and O26. Here we demonstrate that MccPDI can inhibit Shigella strains and E. coli isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion...
Thu, 05/16/2019 - 11:01
Enterotoxigenic Escherichia coli (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and postweaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins could be effective against diarrhea in young pigs. However, developing a vaccine to broadly...

Pages