Bacterial pathogens

Thu, 08/20/2020 - 10:04
ABSTRACT Infections with multidrug-resistant bacteria often leave limited or no treatment options. The transfer of antimicrobial resistance genes (ARG) carrying plasmids between bacterial species by horizontal gene transfer represents an important mode of expansion of ARGs. Here, we demonstrate the application of Nanopore sequencing in a hospital setting for monitoring transfer and rapid...
Thu, 08/20/2020 - 10:03
Symptomatic and asymptomatic infection with the diarrheal pathogen enteroaggregative Escherichia coli (EAEC) is associated with growth faltering in children in developing settings. The mechanism of this association is unknown, emphasizing a need for better understanding of the interactions between EAEC and the human gastrointestinal mucosa. In this study, we investigated the role of the...
Thu, 08/20/2020 - 10:03
Enteric pathogens exploit chemical and nutrient signaling to gauge their location within a host and control expression of traits important for infection. Ethanolamine-containing molecules are essential in host physiology and play important roles in intestinal processes. The transcription factor EutR is conserved in the Enterobacteriaceae and is required for ethanolamine sensing and metabolism. In...
Thu, 08/20/2020 - 02:03
With its ability to easily infect each step of food production chain, Salmonella spp. stands as one of the most important foodborne pathogens which impose threats against economy and human health by creating high morbidity and mortality, worldwide. Although sensitive screening and detection methodologies based on common conventional techniques have been developed for Salmonella spp., their long...
Wed, 08/19/2020 - 22:21
Brucellosis is an important occupational disease, mainly among veterinarians, because of their frequent contact with sick animals, contaminated secretions and live attenuated anti‐Brucella vaccines. This study aimed to determine the prevalence of accidental exposure to S19 and RB51 vaccine strains and occupational brucellosis among veterinarians registered to administer vaccinations in Minas...
Wed, 08/19/2020 - 16:00
Staphylococcus aureus (S. aureus) constantly evolves under host and environment pressures. The monitoring network is essential in assessing the epidemiology of S. aureus infections. A total of 555 S. aureus isolates were collected from five hospitals in three different geographical regions of China for the investigation of molecular characteristics, antibiotic resistance, virulence gene, and wall...
Wed, 08/19/2020 - 10:03
Staphylococcus aureus and other coagulase-positive Staphylococcus spp. bind the Fc region of IgG antibodies through expression of protein A (SpA). These species have consequently been a source of false-positive signals in antibody-based assays designed to detect other target bacteria. Here, flow cytometry was used to study the influence of a number of factors on the SpA-mediated binding of single...
Wed, 08/19/2020 - 10:03
The use of exogenous functional microorganisms to regulate biogenic amine (BA) content is a common approach in fermentation systems. Here, to better understand the microbial traits of succession trajectories in resource-based and biotic interference systems, the BA-related primary and secondary succession were tracked during industrial semidry Chinese rice wine (CRW) fermentation. Dominant...
Wed, 08/19/2020 - 10:03
Interactions between Listeria monocytogenes and food-associated or environmental bacteria are critical not only for the growth but also for a number of key biological processes of the microorganism. In this regard, limited information exists on the impact of other microorganisms on the virulence of L. monocytogenes. In this study, the growth of L. monocytogenes was evaluated in a single culture...
Wed, 08/19/2020 - 10:02
The Food Safety Modernization Act (FSMA) includes a time-to-harvest interval following the application of noncompliant water to preharvest produce to allow for microbial die-off. However, additional scientific evidence is needed to support this rule. This study aimed to determine the impact of weather on the die-off rate of Escherichia coli and Salmonella on spinach and lettuce under field...
Wed, 08/19/2020 - 10:02
Clinical surveillance of enteric pathogens like Salmonella is integral to track outbreaks and endemic disease trends. However, clinic-centered disease monitoring biases toward detection of severe cases and underestimates the incidence of self-limiting gastroenteritis and asymptomatic strains. Monitoring pathogen loads and diversity in municipal wastewater (MW) can provide insight into...
Wed, 08/19/2020 - 08:02
by Lovasoa N. Randriantseheno, Anjanirina Rahantamalala, Ando L. Randrianierenana, Minoarisoa Rajerison, Voahangy Andrianaivoarimanana Background Several tests are available for plague confirmation but bacteriological culture with Yersinia pestis strain isolation remains the gold standard according to the World Health Organization. However, this is a time consuming procedure; requiring specific...
Wed, 08/19/2020 - 08:02
by Amanda Aparecida Seribelli, Marcelo Ferreira Cruz, Felipe Pinheiro Vilela, Miliane Rodrigues Frazão, Mario H. Paziani, Fernanda Almeida, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Marcia R. von Zeska Kress, Marc W. Allard, Juliana Pfrimer Falcão Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) causes gastroenteritis in many countries. However, in...
Wed, 08/19/2020 - 08:02
All cells require Mg2+ to replicate and proliferate. The macrophage protein Slc11a1 is proposed to protect mice from invading microbes by causing Mg2+ starvation in host tissues. However, the Mg2+ transporter MgtB enables the facultative intracellular pathogen Salmonella enterica serovar Typhimurium to cause disease in mice harboring a functional Slc11a1...
Wed, 08/19/2020 - 08:02
Two-component signal transduction systems (TCSs) represent a major mechanism that bacteria use to sense and respond to their environment. Prototypical TCSs are composed of a membrane-embedded histidine kinase, which senses an environmental stimulus and subsequently phosphorylates a cognate partner protein called a response regulator that regulates gene expression in a...
Wed, 08/19/2020 - 08:02
Salmonella enterica is among the most important foodborne pathogens. In Ecuador, there is limited information about non-typhoidal S. enterica occurrence in raw meats, its serotype distribution, and antimicrobial resistance. In this study, we addressed this issue in 1095 retail fresh meats (chicken, pork, veal, lamb, beef, and turkey) in Quito by performing a traditional culture methodology and...
Wed, 08/19/2020 - 06:15
Author(s): Ji Hyun Yoo, Ki Ho Baek, Ye Seul Heo, Hae In Yong, Cheorun Jo We investigated the bactericidal effect of clove oil and encapsulated atmospheric pressure plasma (EAP), individually or in combination, against Escherichia coli O157:H7 and Staphylococcus aureus. The bactericidal effect of the combined treatment was also investigated in inoculated beef jerky. For both pathogens, clove oil...
Tue, 08/18/2020 - 22:19
Escherichia coli O157:H7 entered into the Viable but Non Culturable (VBNC) state under salinity stress of 10 and 30% during production of salted silver carp. VBNC E. coli O157:H7 induced by NaCl stress during salted fish production continued to express its pathogenic genes of stx1, stx2, eae and hly. Abstract Many bacteria including Escherichia coli can enter into the viable but non‐culturable (...
Tue, 08/18/2020 - 22:19
A microfluidic device was applied to enumerate Escherichia coli O157:H7 cells in lettuce and beef samples. E. coli O157:H7 inoculated in lettuce and beef were collected with high efficiency by using filtration or two‐step centrifugation. This method could quantify target microbial cells within 1 hr. Abstract Quality assurance is one of the fundamental ways of preventing infections from foodborne...
Tue, 08/18/2020 - 22:19
Vibrio parahaemolyticus is one of the most hazardous pathogens causing seafood‐borne diseases in the southern Fujian coast, China. From June to October 2016, a total of 250 samples were collected from retail markets in the Xiamen, Quanzhou, and Zhangzhou regions. Seventy‐seven V. parahaemolyticus isolates were identified using polymerase chain reaction (PCR). Then, molecular typing was performed...
Tue, 08/18/2020 - 10:02
Fluctuations in osmolarity are one of the most prevalent stresses to which bacteria must adapt, both hypo- and hyper-osmotic conditions. Most bacteria cope with high osmolarity by accumulating compatible solutes (osmolytes) in the cytoplasm to maintain the turgor pressure of the cell. Vibrio parahaemolyticus, a halophile, utilizes at least six compatible solute transporters for the uptake of...
Tue, 08/18/2020 - 08:04
by Amanda L. Laverty, Sebastian Primpke, Claudia Lorenz, Gunnar Gerdts, Fred C. Dobbs Since plastics degrade very slowly, they remain in the environment on much longer timescales than most natural organic substrates and provide a novel habitat for colonization by bacterial communities. The spectrum of relationships between plastics and bacteria, however, is little understood. The first objective...
Tue, 08/18/2020 - 06:22
Author(s): Marie Noel Mansour, Joseph Yaghi, André El Khoury, Arnaud Felten, Michel-Yves Mistou, Ali Atoui, Nicolas Radomski Salmonella enterica subsp. enterica serovars are considered major causes of food poisoning and we performed this study because Salmonella is a burden in Lebanon. The present study investigated the ability of genomic information to predict serovar using a collection of...
Tue, 08/18/2020 - 02:04
The problems of parasite resistance, as well as the toxic residues to most of the commercially available antipiroplasmic drugs severely weaken their effective, curative, and environmental safe employment. Therefore, it is clear that the development of treatment options for piroplasmosis is vital for improving disease treatment and control. Ciprofloxacin is a broad-spectrum antibiotic that targets...
Mon, 08/17/2020 - 22:17
Listeria monocytogenes, a well‐known foodborne pathogen and the causative agent of listeriosis, has the ability to persist in food processing environments due to its high adhesion ability in different surfaces, playing an important role in the food industry. The aim of this study was to assess how the main stressing conditions, usually observed in meat processing facilities (sanitizers, NaCl,...
Mon, 08/17/2020 - 22:16
The dissemination and abundances of Vibrio species in aquatic environments are of interest, as some species cause emerging diseases in humans and in aquatic organisms like fish. It is suggested that Vibrio cholerae non‐O1 infections of Plecoglossus altivelis (‘ayu’) were spread to various parts of Japan through the annual transplantation of juvenile fish. To investigate this, we used genome‐aided...
Mon, 08/17/2020 - 22:15
Salmonella enterica serovar Typhimurium (S. Typhimurium, STM) is a leading cause of non‐typhoidal salmonellosis (NTS) worldwide. The pathogenesis of NTS has been studied extensively using a streptomycin‐pretreated mouse colitis model with the limited numbers of laboratory S. Typhimurium strains. However, the pathogenicity of the clinically isolated S. Typhimurium (STMC) strains endemic in...
Mon, 08/17/2020 - 16:00
The chemotherapeutic options for methicillin-resistant Staphylococcus aureus (MRSA) infections are limited. Due to the multiple resistant MRSA, therapeutic failure has occurred frequently, even using antibiotics belonging to different categories in clinical scenarios, very recently. This study aimed to investigate the interactions between 11 antibiotics representing different mechanisms of action...
Mon, 08/17/2020 - 14:07
Salmonella enterica serovar Pullorum (S. Pullorum) is an important pathogen specific to avian species, which poses a serious threat to the poultry industry. The transmission of S. Pullorum occurs both horizontally and vertically but the airborne transmission of S. Pullorum has been neglected historically. In this study, the effects of aerosolized S. Pullorum on young chickens were investigated....
Mon, 08/17/2020 - 06:17
Author(s): Zhongguo Shan, Yanpeng Yang, Ning Guan, Xiaodong Xia, Weidong Liu The extensive use of antibiotics in aquaculture has resulted in the prevalence of antibiotic-resistant bacteria and, consequently, new antibacterial strategies or drugs with clear modes of action are urgently needed. Antimicrobial peptides (AMPs) are currently widely considered as alternatives to antibiotics in the...
Mon, 08/17/2020 - 06:16
Author(s): Mengyuan Qi, Ruoqing Zhao, Qingyan Liu, Haiyang Yan, Yan Zhang, Shiyi Wang, Yuan Yuan Inhibitory effects of three kinds of electric fields, high voltage electrostatic field (HVEF), alternating electric field (HVAEF) and prick electrostatic field (HVPEF) against Staphylococcus aureus (S. aureus) were investigated in medium plates and food systems. The results showed that S. aureas was...
Sun, 08/16/2020 - 06:15
Author(s): Ruixue Chang, Pramod Pandey, Yanming Li, Chandrasekar Venkitasamy, Zhao Chen, Rodrigo Gallardo, Bart Weimer, Michele Jay-Russell, Bart Weimer Poultry litter is used as soil amendment or organic fertilizer. While poultry litter is enriched with organic matter suitable for land, the presence of pathogens such as Salmonella in poultry litter is a concern. To investigate the effect of...
Sun, 08/16/2020 - 06:14
Author(s): Amaia Lasagabaster, Elisa Jiménez, Tatiana Lehnherr, Katherine Miranda-Cadena, Hansjörg Lehnherr Listeria monocytogenes is a well-known pathogen responsible for the severe foodborne disease listeriosis. The control of L. monocytogenes occurrence in seafood products and seafood processing environments is an important challenge for the seafood industry and the public health sector....
Sun, 08/16/2020 - 06:12
Author(s): Baikui Wang, Altaf Hussain, Yuanhao Zhou, Zihan Zeng, Qi Wang, Peng Zou, Li Gong, Pengwei Zhao, Weifen Li Macrophages are professional phagocytic cells that play a critical role in initiating immune responses by presenting antigen and phagocytic clearance. The macrophages can be targeted for immunomodulation by beneficial microbes, such as probiotics. The aim of this study is to...
Sun, 08/16/2020 - 06:12
LWT
Author(s): Shruti Chatterjee, Ishan Raval, Krishna Raval, Rima Tapader, Gopal Bhojani, Amit Pal, Sandhya Mishra This study intended to determine whether edible microalgae can play an active role as an anti-virulence agent for enteropathogens. It showed that Chlorella variabilis (ATCC PTA 12198) are able to inhibit the production of the virulence factors in pathogenic Vibrio cholerae strains, the...
Sun, 08/16/2020 - 06:12
LWT
Author(s): Abeni Beshiru, Isoken H. Igbinosa, Etinosa O. Igbinosa The present study was carried out to determine and characterize the virulence and antimicrobial resistance profile of S. aureus from ready-to-eat (RTE) shrimp. A total of 720 RTE shrimp samples from open market in Delta State Nigeria were assessed for the virulence and antimicrobial characteristic profile of S. aureus using...
Sun, 08/16/2020 - 06:12
LWT
Author(s): Jae-Hyun Yoon, Hyeonjin Chu, Do-Young Jeong, Songyi Choi, In-Jun Hwang, Sun-Young Lee, Se-Ri Kim This study was undertaken to examine the effect of a 405-nm light-emitting diode (LED) illumination alone or combined with 3% organic acid dipping on the survival of Listeria monocytogenes in enoki mushrooms stored at 5 °C. Less than 1.0 or 2.1–4.4 log-reductions were achieved when L....
Sat, 08/15/2020 - 22:17
ES24, but not nitrofurantoin, inhibits SecYEG‐dependent protein transport in bacteria and exhibits anti‐bacterial activity towards various clinically relevant strains. In addition, ES24 affects many other processes important to E. coli, hinting at a multifaceted mode of action of ES24. Summary Eeyarestatin 1 (ES1) is an inhibitor of endoplasmic reticulum (ER) associated protein degradation, Sec61...
Sat, 08/15/2020 - 16:00
Typically, raw meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of meat contaminated with antibiotic resistant E. coli is associated with grave hea...
Sat, 08/15/2020 - 12:00
Background Campylobacter infection is associated with impaired growth of children, even in the absence of symptoms. To examine the underlying mechanisms, we evaluated associations between Campylobacter infection, linear growth, and fecal microbial community features in a prospective birth cohort of 271 children with a high burden of diarrhea and stunting in the Amazonian lowlands of Peru. Methods...
Sat, 08/15/2020 - 08:04
Host organisms utilize nutritional immunity to limit the availability of nutrients essential to an invading pathogen. Nutrients may include amino acids, nucleotide bases, and transition metals, the essentiality of which varies among pathogens. The mammalian macrophage protein Slc11a1 (previously Nramp1) mediates resistance to several intracellular pathogens. Slc11a1 is proposed to restrict growth...
Sat, 08/15/2020 - 06:25
Author(s): Jiahui Li, Siqi Li, Haozhou Li, Xinyu Guo, Du Guo, Yanpeng Yang, Xin Wang, Chunling Zhang, Zhongguo Shan, Xiaodong Xia, Chao Shi Shikonin is a bioactive compound found in the roots of Lithospermum erythrorhizon and has demonstrated antibacterial activity. In the current study, we aimed to examine the antibiofilm activity of shikonin against Listeria monocytogenes, and to investigate...
Sat, 08/15/2020 - 06:21
Author(s): Xiaobao Qi, Zhilong Wang, Rongsheng Lu, Jiawei Liu, Yue Li, Yiping Chen Early screening of L. monocytogenes in ready-to-eat food can prevent and control its harmful effects. In this study, we propose a highly sensitive magnetic DNA sensor based on nucleic acid hybridization reaction and magnetic signal readout. We design the L. monocytogenes specific probe1 and probe2 and label them on...
Sat, 08/15/2020 - 04:02
A strain of thiamethoxam-degrading endophyte, named TMX13, was isolated from roots of mulberry (Morus alba L.) and was identified as Enterobacter sp. Inoculating Chinese cabbage (Brassica chinensis L) with strain TMX13-gfp (gfp-labeled TMX13) could significantly reduce thiamethoxam residues in the aboveground part (edible portion) of the vegetable. The theoretical daily intake (TDI) of...
Fri, 08/14/2020 - 22:20
This study was aimed to investigate the presence of Listeria monocytogenes in raw water buffalo milk and milk products, besides determining its serotype and the extent of its resistance against various antibiotics. A total of 188 samples of raw water buffalo milk and milk products were collected from Samsun Province, Turkey between November 2012 and May 2013. The classical culture technique was...
Fri, 08/14/2020 - 22:18
A dietary supplement composed of lactoferrin hydrolysate (LfH), whey protein hydrolysate (WPH) and vanillin showed antibacterial activity against E.coli and S.aureus in vitro. The studied supplement also exhibited significant antioxidant properties in the model system and anti‐oxidative stress activity in mice exposed to paclitaxel. This supplement has a potential for use in the food matrix to...
Fri, 08/14/2020 - 16:00
The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance (AMR) crisis furthermore. Here, tigecycline-resistant Enterobacterales strains were isolated and detected with carbapenemase genes,...
Fri, 08/14/2020 - 16:00
Antimicrobial resistance is an ancient bacterial defense mechanism that has rapidly spread due to the frequent use of antibiotics for disease treatment and livestock growth promotion. We are becoming increasingly aware that pathogens, such as members of the genus Acinetobacter, are precipitously evolving drug resistances through multiple mechanisms, including the acquisition of antibiotic...
Fri, 08/14/2020 - 16:00
Daptomycin (DAP) is one of the last-resort treatments for heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) infections. DAP resistance (DAP-R) is multifactorial and mainly related to cell-envelope modifications caused by single-nucleotide polymorphisms and/or modulation mechanisms of transcription emerging as result of a self-defense...
Fri, 08/14/2020 - 06:15
Author(s): Jiliang Lin, Junhao Ning, Xia Lu, Min Chen, Weian Cao, Chunde Wang Little information is available on innate immune defense mechanisms of Scapharca subcrenata. C-type lectins (CTLs) are not only pattern recognition proteins that can bind pathogen-associated molecular patterns, but also crucial maternally-derived immune factors in mollusc egg. In this study, the comparative...

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