Bacterial pathogens

Tue, 01/14/2020 - 03:23
Dairy product safety is a global public health issue that demands new approaches and technologies to control foodborne pathogenic microorganisms. Natural antimicrobial agents such as nisin can be added to control the growth of pathogens of concern in dairy foods, namely Listeria monocytogenes and Staphylococcus aureus. However, several factors affect the antimicrobial efficacy of nisin when...
Mon, 01/13/2020 - 03:28
LWT
Author(s): Yifang Cao, Ailian Zhou, Donggen Zhou, Xinglong Xiao, Yigang Yu, Xiaofeng LiAbstractResponse mechanism of Cronobacter sakazakii under stress of combination of citral and carvacrol was investigated by transcriptome sequencing. Twenty-five significantly differentially expressed genes were identified to be closely related to the ability of C. sakazakii tolerating the stress after analysis...
Mon, 01/13/2020 - 03:28
LWT
Author(s): Ramachandrappa Naveen Kumar, Bhukya Bhima, Putchaa Uday Kumar, Sudip GhoshAbstractSalmonella spp., one of the major causes of foodborne diseases and a public health burden worldwide. Lytic bacteriophages NINP13076 and NINP1162 infecting Salmonella spp were isolated from sewage water with host strains Salmonella enteritidis (ATCC 13076) and Salmonella spp (MTCC 1162) respectively. The...
Sun, 01/12/2020 - 03:33
Author(s): Felipe Pinheiro Vilela, Carolina Nogueira Gomes, Mário Henrique Paziani, Vânia Santos Braz, Dália dos Prazeres Rodrigues, Renata Garcia Costa, Monique Ribeiro Tiba Casas, Marcia Regina von Zeska Kress, Juliana Pfrimer Falcão, Fábio CampioniAbstractSalmonella Dublin is a strongly cattle-adapted serovar that is also responsible for severe invasive infections in humans. Although invasive...
Sun, 01/12/2020 - 03:32
Author(s): Zhen Jia, Yabo Peng, Xiaotong Yan, Ziye Zhang, Ting Fang, Changcheng LiAbstractThe present study was aimed at developing a mathematical model to predict the growth of Salmonella in ground chicken in the presence of the background microflora. Ground chicken was inoculated with a cocktail of S. Typhimurium and S. Enteritidis and incubated at various isothermal temperatures (8-33 °C)....
Sun, 01/12/2020 - 03:32
Author(s): Chujun Ou, Daiqi Shang, Jingxian Yang, Bo Chen, Jiang Chang, Fangning Jin, Chunlei ShiAbstractAntimicrobial resistance has gradually become a serious problem threatening public health and food safety throughout the world. Biofilm is one of the important factors affecting the antimicrobial resistance of bacteria. Staphylococcus aureus usually has strong biofilm formation ability, and it...
Sun, 01/12/2020 - 03:30
Author(s): Song Zhang, Jian Xiong, Wenyong Lou, Zhengxiang Ning, Denghui Zhang, Jiguo YangAbstractCronobacter sakazakii (C. sakazakii) is an opportunistic foodborne pathogen in infant formula. This study was designed to explore the inhibitory effect of TGML on C. sakazakii in reconstituted infant formula (RIF). Firstly, the growth curve of C. sakazakii in RIF treated by TGML and the effect of...
Sat, 01/11/2020 - 03:26
Alpha diversity boxplots showing the distribution of Chao1 diversity for produce items. The Chao1 alpha diversity is presented for samples from farm fresh (cucumbers, tomatoes, and kale) and organic (squash and green bell peppers) produce items collected 1, spiepr146 24, and 96 hr after treatment with water, chlorine, PAA, or SalmoFresh. Abstract Diets rich in minimally processed foods are...
Sat, 01/11/2020 - 03:26
Salmonella were detected in 3 of 24 cucumbers, all of which were multidrug‐resistant and one was acid‐resistant. In addition, developed dynamic model showed that the Salmonella isolates proliferated gradually in cucumber under changing temperature, simulating storage condition. These results suggest that cross‐contaminated Salmonella could increase during storage. It may lead to human infection,...
Sat, 01/11/2020 - 03:25
Aim Following previous research on improving the cleaning of crates used to transport broiler chickens from the farm to the abattoir, a demonstration project was undertaken to investigate improvements in crate washing on a commercial scale. Methods and Results The soak tank of a conventional crate washing system was replaced with a high‐performance washer fitted with high‐volume, high‐pressure...
Sat, 01/11/2020 - 03:25
The efficacy of benzyl isothiocyanate (BIT), a compound found in cruciferous vegetables, for controlling Salmonella on alfalfa seeds and sprouts was investigated. Alfalfa seeds were inoculated by individual Salmonella enterica serotypes Newport, Typhimurium, Tennessee, Montevideo, or Braenderup at two inoculation levels of 3 or 5 log CFU g‐1, followed by treatment with BIT (0.5%‐2%) or chlorine (...
Sat, 01/11/2020 - 03:23
Salmonella Gallinarum only infects avian species, where it causes a severe systemic infection in birds of all ages. It is generally accepted that interaction with phagocytic cells plays an important role in the development of systemic, host-specific Salmonella infections. The current study detailed the interaction of S. Gallinarum with macrophages derived from chicken (HD11) and cattle (Bomac)...
Sat, 01/11/2020 - 03:23
Clostridium perfringens is an important human and animal pathogen that is the primary causative agent of necrotizing enteritis and enterotoxemia in many types of animals; it causes traumatic gas gangrene in humans and animals and is associated with cases of food poisoning in humans. C. perfringens produces a variety of toxins as well as many enzymes, including three sialidases, NanH, NanI, and...
Sat, 01/11/2020 - 03:23
Avian botulism is a serious neuroparalytic disease mainly caused by a type C/D botulinum neurotoxin produced by Clostridium botulinum group III, one of the entwined bacterial species from the Clostridium novyi sensu lato genospecies. Its isolation is very challenging due to the absence of selective media and the instability of the phage carrying the gene encoding for the neurotoxin. The present...
Fri, 01/10/2020 - 03:34
Hybrid assembly of Illumina and Oxford Nanopore sequencing was used here to produce the complete circular genome and plasmid sequences of Salmonella enterica subsp. enterica serovar Enteritidis PT1 (phage type 1). The organism was obtained from the Salmonella Reference Laboratory at Public Health England, Colindale, UK.
Fri, 01/10/2020 - 03:31
Author(s): Vinaya Kizhakkepatt Kizhakkekalam, Kajal Chakraborty, Minju JoyABSTRACTThree homologous oxygenated elansolid-type of polyketide spanned macrolides were isolated from a heterotrophic marine bacterium, Bacillus amyloliquefaciens MTCC 12716 associated with an intertidal red alga Hypnea valentiae. We have sequenced the complete genome of the bacterium and analyzed all detectable natural...
Fri, 01/10/2020 - 03:31
Author(s): Chunling Zhang, Zhiyi Zhao, Gaoji Yang, Yiqi Shi, Yuyu Zhang, Xiaodong Xia, Chao ShiAbstractMicrobial contamination of sprouts occurs easily because of the pathogens present on and in the seeds and the optimal conditions for bacteria growth provided during the germination and sprouting processes. This study examined the effect of using slightly acidic electrolyzed water (SAEW), a ‘...
Fri, 01/10/2020 - 03:30
Author(s): Yaodi Zhu, Jiaye Zhang, Miaoyun Li, Lijun Zhao, Hongrong Ren, Longgnag Yan, Gaiming Zhao, Chaozhi ZhuAbstractThe Gram-positive, anaerobic, spore-forming bacterium, Clostridium perfringens (C. perfringens) causes a variety of diseases in humans and other animals. Spore germination is thought to be the first stage of infection by C. perfringens. AGFK, a mixture of l-asparagine, d-glucose...
Fri, 01/10/2020 - 03:30
Author(s): Dongyun Jung, Joseph E. RubinAbstractThe role of plant-based foods in the epidemiology of antimicrobial resistance has been inadequately studied. In this investigation, resistant organisms from vegetables, fruits and spices imported into Canada were identified and characterized. A total of 143 products imported from primarily Asian and African countries were purchased from...
Fri, 01/10/2020 - 03:30
Author(s): Olouwafemi Mistourath Mama, Liliana Morales, Laura Ruiz-Ripa, Myriam Zarazaga, Carmen TorresAbstractMethicillin-resistant Staphylococcus aureus (MRSA) CC398 is a livestock-associated (LA) lineage, mainly detected in swine. Its dissemination via the food-chain could be a food-safety issue. This work aimed to study the diversity of S. aureus lineages in pork-products, to determine the...
Fri, 01/10/2020 - 03:30
Author(s): Anastasia E. Lytou, Chrissanthi T. Renieri, Agapi I. Doulgeraki, George-John E. Nychas, Efstathios Z. PanagouAbstractThe prevalence of three pathogens in marinated chicken products and the evaluation of their quality by microbiological and sensory analysis were assessed. Eighty (80) samples obtained from several meat retail markets in Greece were analyzed for the presence of...
Fri, 01/10/2020 - 03:29
LWT
Author(s): Zijin Zhou, Sophie Zuber, Matteo Campagnoli, Mireille Moser, Frank Devlieghere, Mieke UyttendaeleAbstractDrying technologies are often evaluated on their efficiency to remove moisture and impact on sensory quality of products. The decontamination effect against foodborne pathogens, however, is rarely investigated. This study investigated the inactivation of foodborne pathogens and...
Fri, 01/10/2020 - 03:29
LWT
Author(s): Qile Yang, Yifeng Ding, Ruonan Nie, Lan Yao, Xinyue Wang, Min Zhou, Jia Wang, Xiaohong WangAbstractSalmonella Typhimurium, one of the most common Salmonella spp. serovars, is recognized as a globally important foodborne pathogen. In this study, bacteriophage LPST144 against S. Typhimurium (ATCC13311) was isolated from sewage and selected owing to its excellent lytic capacity. After...
Fri, 01/10/2020 - 03:23
Campylobacter can enter a viable but non-culturable (VBNC) state to evade various stresses, and this state is undetectable using traditional microbiological culturing techniques. These VBNC bacterial cells retain metabolism and demonstrate pathogenic potential due to their ability to resuscitate under favorable conditions. Rapid and accurate determination of VBNC Campylobacter is critical to...
Thu, 01/09/2020 - 04:09
ABSTRACT Carbapenemases confer resistance to nearly all β-lactam antibiotics. The extensive spread of carbapenemase-producing multidrug-resistant bacteria contributes significantly to hospital-acquired infections. We have developed a novel protein-based binding assay that identifies KPC β-lactamases from clinical isolates. We used the protein-protein interaction between KPCs and a...
Thu, 01/09/2020 - 04:09
ABSTRACT IS26 has been shown to form cointegrates both by a copy-in mechanism involving one insertion sequence (IS) and a target and by a targeted conservative mechanism involving two ISs. IS26 is the flagship of a group of 65 bacterial ISs in the recently redefined IS6/IS26 family. Here, whether other family members can also use two mechanisms was examined using members of the IS257/IS431 and...
Thu, 01/09/2020 - 04:09
ABSTRACT The survival ability of Salmonella enterica serovar Enteritidis in antibacterial egg white is an important factor leading to Salmonella outbreaks through eggs and egg products. In this study, the role of the gene yoaE, encoding an inner membrane protein, in the survival of Salmonella Enteritidis in egg white, and its transcriptional regulation by CpxR were investigated. Quantitative...
Thu, 01/09/2020 - 04:06
Author(s): Ada Kazi, Che Muhammad Khairul Hisyam Ismail, Anthony Amy Amilda, Candy Chuah, Chiuan Herng Leow, Boon Huat Lim, Kirnpal Kaur Banga Singh, Chiuan Yee LeowAbstractShigellosis is one of the most common diseases found in the developing countries, especially those countries that are prone flood. The causative agent for this disease is the Shigella species. This organism is one of the third...
Thu, 01/09/2020 - 04:05
Author(s): Hourong Liu, Chengwen Song, Junhao Ning, Yuan Liu, Zhaoxia CuiAbstractVariable lymphocyte receptors (VLRs) play an important role via their antigen-special reorganization in jawless vertebrates (agnathans) adaptive immune response. In the present study, the open reading frame (ORF) of Eriocheir sinensis VLRA (designated as EsVLRA) was identified. EsVLRA comprised a 799-amino-acid...
Thu, 01/09/2020 - 04:05
Author(s): Hoang Minh Duc, Hoang Minh Son, Hazel Pang Shu Yi, Jun Sato, Pham Hong Ngan, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa MiyamotoAbstractSalmonella Enteritidis, Salmonella Typhimurium, and Escherichia coli O157:H7 are the most important foodborne pathogens, causing serious food poisoning outbreaks worldwide. Bacteriophages are increasingly considered as novel antibacterial agents to...
Thu, 01/09/2020 - 04:04
Author(s): Veerachandra K. Yemmireddy, Cameron Cason, Juan Moreira, Achyut AdhikariAbstractThe shells of pecans are a rich source of bioactive compounds with potential inhibitory activity against various pathogenic microorganisms. This study investigated the antimicrobial activity of pecan shell extracts as effected by the type of cultivar and the method of extraction against various foodborne...
Thu, 01/09/2020 - 04:04
Author(s): Simon Packer, Jane Day, Peter Hardman, Julia Cameron, Michael Kennedy, Jonathan Turner, Caroline Willis, Corinne Amar, Bayad Nozad, Maya GobinAbstractIn March 2018, Public Health England was alerted to an outbreak of gastrointestinal illness in persons who had eaten at a restaurant in the South West of England on Mothering Sunday. We aimed to determine the source of infection and...
Thu, 01/09/2020 - 04:03
Author(s): Ruchi Sharma, Seid Mahdi Jafari, Somesh SharmaAbstractBio-nanocomposites are bio-based polymers made up of two major components in which one acts as a matrix called biopolymer (continuous phase) and second called as reinforcement agent (dispersed phase) with dimensions in the range of 1–100 nm. These exhibit typical characteristics such as flexibility, biocompatibility,...
Thu, 01/09/2020 - 04:03
Author(s): Torrung Vetchapitak, Taisuke Shinki, Satomi Sasaki, Takako Taniguchi, Taradon Luangtongkum, Naoaki MisawaAbstractThe present study was conducted to evaluate the efficacy of a new technique for removal of campylobacters from naturally contaminated free-range and broiler chicken carcasses. The carcasses, obtained from commercial processing plants after evisceration, were immersed in 0.1...
Thu, 01/09/2020 - 04:02
Author(s): Xing-Ping Li, Ruan-Yang Sun, Jia-Qi Song, Liang-Xing Fang, Rong-Min Zhang, Xin-Lei Lian, Xiao-Ping Liao, Ya-Hong Liu, Jun Lin, Jian SunABSTRACTObjectivesTo characterize the colistin-resistant bacterial population in the gut and assess diversity of mcr-1 transmission within a single individual.MethodsLarge numbers of isolates (>100 colonies/chicken cecum sample) were collected from...
Thu, 01/09/2020 - 03:58
Author(s): Prince Pal Singh, Satparkash Singh, B.V. Sunil Kumar, R.S. Sethi, Ramneek VermaAbstractMonitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red...
Thu, 01/09/2020 - 03:53
Campylobacter species are known as biofilm‐forming bacteria in food systems. The aim of this study was to evaluate the antimicrobial and antibiofilm effects of cinnamaldehyde against Campylobacter jejuni and Campylobacter coli isolated from chicken meat. The biofilm‐forming C. jejuni and C. coli strains from chicken meat were investigated using minimum inhibitory concentration (MIC) and...
Thu, 01/09/2020 - 03:46
Aquatic pathogen Aeromonas hydrophila produces an array of virulence factors, many of which are excreted proteins that causes infectious disease in fish, reptiles, and humans. Aerolysin, a haemolytic toxin, is the most well-known of the A. hydrophila virulence factors and is encoded by aerA. Although used as a virulence gene marker in several studies, recent whole-genome sequencing data suggest...
Thu, 01/09/2020 - 03:44
ABSTRACT The zoonotic disease Q fever caused by the intracellular bacterium Coxiella burnetii remains a global health threat due to its high infectivity, environmental stability, the debilitating nature and the long duration of treatment. Designing new and potent drugs that target previously unexplored pathways is essential to shorten treatment time and minimise antibiotic resistance....
Thu, 01/09/2020 - 03:42
The emergence of extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli represent a contemporary public health threat. ESBL and AmpC β-lactamase genes translocate between chromosomes and plasmids, facilitating rapid spread throughout the environment. In this study, ESBL/AmpC producing bacteria were isolated from beef cattle farms with seldom antibiotic use. Eleven...
Thu, 01/09/2020 - 03:42
Given that recent advances in metagenomics have highlighted the importance of intestinal microbes for poultry health, there has been a corresponding search for early manipulation strategies of intestinal microbiota in order to advance immune system development and optimize functional properties of growth. In this study, we used the in ovo technique as an experimental model to address how early...
Thu, 01/09/2020 - 03:42
The prevalence of human infections with the zoonotic pathogen Campylobacter jejuni is rising worldwide. Therefore, the identification of compounds with potent anti-pathogenic and anti-inflammatory properties for ...
Wed, 01/08/2020 - 03:41
by Markus Beutler, Sara Plesnik, Marina Mihalic, Laura Olbrich, Norbert Heinrich, Samuel Schumacher, Michael Lindner, Ina Koch, Wolfgang Grasse, Christoph Metzger-Boddien, Sabine Hofmann-Thiel, Harald Hoffmann Rapid diagnosis of tuberculosis (TB) and antibiotic resistances are imperative to initiate effective treatment and to stop transmission of the disease. A new generation of more sensitive,...
Wed, 01/08/2020 - 03:41
by Jilong Qin, Matthew Thomas Doyle, Elizabeth Ngoc Hoa Tran, Renato Morona Shigella species cause bacillary dysentery, especially among young individuals. Shigellae target the human colon for invasion; however, the initial adhesion mechanism is poorly understood. The Shigella surface protein IcsA, in addition to its role in actin-based motility, acts as a host cell adhesin through unknown...
Wed, 01/08/2020 - 03:40
Steryl esters (SEs) are important storage compounds in many eukaryotes and are often prominent components of intracellular lipid droplets. Here, we demonstrate that selected Actino- and Proteobacteria growing on sterols are also able to synthesize SEs and to sequester them in cytoplasmic lipid droplets. We found cholesteryl ester (CE) formation in members of the actinobacterial genera Rhodococcus...
Wed, 01/08/2020 - 03:40
The organoarsenical feed additive 4-hydroxy-3-nitrobenzenearsonic acid (roxarsone [ROX]) is widely used and released into the environment. We previously showed a two-step pathway of ROX transformation by Enterobacter sp. strain CZ-1 involving the reduction of ROX to 3-amino-4-hydroxyphenylarsonic acid (3-AHPAA) and the acetylation of 3-AHPAA to N-acetyl-4-hydroxy-m-arsanilic acid (N-AHPAA) (K....
Wed, 01/08/2020 - 03:40
This study aimed to evaluate the prevalence and genetic characteristics of carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage and river water in the Philippines, which has a typical tropical maritime climate. We collected 83 water samples from 7 hospital sewage and 10 river water sites. CPE were identified using CHROMagar mSuperCARBA, and Gram-negative strains were identified...

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