Bacterial pathogens

Tue, 09/17/2019 - 12:01
In bacteria, a primary σ-factor associates with the core RNA polymerase (RNAP) to control most transcription initiation, while alternative σ-factors are used to coordinate expression of additional regulons in response to environmental conditions. Many alternative σ-factors are negatively regulated by anti–σ-factors. In Escherichia coli, Salmonella enterica, and many other γ-proteobacteria,...
Tue, 09/17/2019 - 12:01
MICROBIOLOGY Correction to Supporting Information for “Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi,” by Florence Caro, Nicole M. Place, and John J. Mekalanos, which was first published August 1, 2019; 10.1073/pnas.1906158116 (Proc. Natl. Acad. Sci. U.S.A. 116, 17013–17022). The authors note that Fig. S14 in the SI...
Tue, 09/17/2019 - 11:14
Abstract This study investigated the existence of sulfonamides and colistin resistance genes among extended‐spectrum beta‐lactamase (ESBL)‐producing E. coli recovered from fish gut in Vietnam and evaluated the susceptibility patterns of the ESBL‐producing E. coli to relevant antimicrobials. A total of 88 ESBL‐producing E. coli isolates were analyzed for the presence of the ESBLs, sul (1,2,3) and...
Tue, 09/17/2019 - 11:00
The relationship between porcine gut microbiota composition and health is an important area of research, especially due to the need to find alternatives to antimicrobial use to manage disease in livestock production systems. Previous work has indicated that lower crude dietary protein levels can reduce the impacts of postweaning colibacillosis, which is a porcine diarrheal disease caused by...
Tue, 09/17/2019 - 11:00
l-Aspartate has been widely used in medicine and the food and chemical industries. In this study, Serratia marcescens maleate cis-trans isomerase (MaiA) and Escherichia coli aspartase (AspA) were coupled and coexpressed in an engineered E. coli strain in which the byproduct metabolic pathway was inactivated. The engineered E. coli strain containing the dual-enzyme system (pMA) was employed to...
Tue, 09/17/2019 - 11:00
Bacteria synthesize amino acids according to their availability in the environment or, in the case of pathogens, within the host. We explored the regulation of the biosynthesis of branched-chain amino acids (BCAAs) (l-leucine, l-valine, and l-isoleucine) in Vibrio alginolyticus, a marine fish and shellfish pathogen and an emerging opportunistic human pathogen. In this species, the ilvGMEDA operon...
Tue, 09/17/2019 - 11:00
Salmonella enterica subsp. enterica serovar Enteritidis is able to adapt to sublethal concentrations of ethanol, which subsequently induce tolerance of this pathogen to normally lethal ethanol challenges. This work aims to elucidate the underlying ethanol adaptation mechanisms of S. Enteritidis by proteomic and mutagenic analyses. The global proteomic response of S. Enteritidis to ethanol...
Tue, 09/17/2019 - 10:58
Abstract Clostridium perfringens is the main cause of sudden death in dogs and currently there is no vaccine to prevent it. In this study, a canine C. perfringens type A strain was used to prepare a vaccine. C. perfringens was inactivated by formaldehyde and adjuvants were added. The safety and immunological characteristics of the inactivated C. perfringens vaccine were evaluated in mice and dogs...
Tue, 09/17/2019 - 01:03
Available online 16 September 2019 Author(s): Yuan Fang, Lynn M. McMullen, Michael G. GänzleAbstractDrying processes do not eliminate pathogenic Escherichia coli in foods but induce sublethal injury, which may also induce the Shiga toxin (Stx) prophage. This study investigated the effect of drying on membrane lipid oxidation and stx expression in E. coli. Lipid peroxidation was probed with C11-...
Tue, 09/17/2019 - 01:03
Publication date: Available online 15 September 2019 Food and Chemical ToxicologyAuthor(s): J. Pitt, M. Chan, C. Gibson, O. Hasselwander, A. Lim, P. Mukerji, R. Mukherjea, A. Myhre, P. Sarela, P. Tenning, M.W. Himmelstein, J.M. RoperAbstract3-Fucosyllactose (3-FL), a highly abundant complex carbohydrate in human breast milk, functions as a prebiotic promoting early microbial colonization of the...
Mon, 09/16/2019 - 01:05
Available online 14 September 2019 Author(s): Victoria Stadler Tasca Ribeiro, Letícia Kraft, Paula Hansen Suss, Juliette Cieslinski, Luciana Cristina Wollmann, Felipe Francisco TuonAbstractAlthough reports of infections caused by anaerobes after tissue transplantation are uncommon, contamination of allografts may result in substantial complications. Anaerobic incubation and testing of organ...
Mon, 09/16/2019 - 00:00
Abstract Spices are primarily used as flavor enhancers and have attracted attention as natural food preservatives since their antimicrobial effects were determined. In the present study, the antimicrobial effects, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values on 5 important food-borne pathogenic bacteria were investigated in 20...
Sun, 09/15/2019 - 01:03
Available online 14 September 2019 Author(s): Elpiniki Vandera, Maria Parapouli, Athanasia Kakouri, Anna-Irini Koukkou, Efstathios Hatziloukas, John SamelisABSTRACTThe presence of eight common structural enterocin genes, singly or in varying combinations, in the genome of 15 antagonistic Enterococcus spp. previously isolated from artisan Greek Graviera and Galotyri retail cheeses was tested and...
Sat, 09/14/2019 - 01:03
Available online 12 September 2019 Author(s): Khursheed Ahmad Shiekh, Soottawat BenjakulAbstractPacific white shrimp with prior pulsed electric field (PEF) treatment before soaking in Chamuang leaf extract (CLE) at different concentrations (0.5 and 1%) for 30 min were prepared. Sample pre-treated with PEF and soaked with 1% CLE (PEF-1 CLE) showed lower melanosis score than that with 1.25% sodium...
Sat, 09/14/2019 - 01:03
Available online 12 September 2019 Author(s): Selin Kalkan, Mustafa Remzi Otağ, Mehmet Soner EnginAbstractMethylcellulose (MC) polymer was used to prepare the edible films. Then, the Rheum ribes L. ethanol extract (RE) was added to the films in order to act as a natural antimicrobial agent. All the methylcellulose films were characterized for bioactivity and the physicochemical analysis were...
Sat, 09/14/2019 - 01:03
LWT
Publication date: January 2020Source: LWT, Volume 117Author(s): Li-Wah Wong, Chih-Yao Hou, Chun-Chi Hsieh, Chao-Kai Chang, Yi-Shan Wu, Chang-Wei HsiehAbstractThis research is mainly focused on preparing gallic acid (GA) coated, antimicrobial, active packaging, low density polyethylene (LDPE) films by capacitively coupled plasma. Firstly, the suitable plasma treatment condition was 30 W treated...
Fri, 09/13/2019 - 20:00
Recent evidence suggests that the commensal microbes act as a barrier against invading pathogens and enteric infections are the consequences of multi-layered interactions among commensals, pathogens, and the h...
Fri, 09/13/2019 - 01:03
Available online 11 September 2019 Author(s): Mary Theresa Callahan, Shirley A. MicallefAbstractA dearth of knowledge exists on the pathogen-commodity association of Salmonella enterica on cucumber, despite cucumbers being implicated in multiple salmonellosis outbreaks in recent years in the U.S. Data are lacking on cultivar susceptibility to Salmonella colonization in relation to heterogeneity...
Fri, 09/13/2019 - 01:03
Available online 11 September 2019 Author(s): Xiang-Feng Zheng, Zhen-quan Yang, Hui Zhang, Wen-Xi Jin, Cui-Wen Xu, Lu Gao, Sheng-Qi Rao, Xin-an JiaoAbstractPickle is a type of mildly lactic acid fermented vegetable and is a traditional dish favored in China, Japan, and Korea. Corruption of spoilage bacteria and accumulation of nitrite during vegetable fermentation are common problems that affect...
Fri, 09/13/2019 - 01:03
Available online 11 September 2019 Author(s): Claudine Fournier, Marta Aires-de-Sousa, Patrice Nordmann, Laurent PoirelAbstractBackgroundOur study aimed to prospectively analyze the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs in two farms in Portugal, and to evaluate the putative correlations with different antibiotic usages...
Fri, 09/13/2019 - 00:00
Toxins, Vol. 11, Pages 534: Impact of the Lipopolysaccharide Chemotype of Salmonella Enterica Serovar Typhimurium on Virulence in Gnotobiotic Piglets Toxins doi: 10.3390/toxins11090534 Authors: Alla Splichalova Zdislava Splichalova Daniela Karasova Ivan Rychlik Paolo Trevisi Marek Sinkora Igor Splichal Salmonella Typhimurium is an enteric pathogen that causes acute and chronic...
Thu, 09/12/2019 - 20:00
Many pathogenic bacteria use the type III secretion system (T3SS) injectisome to manipulate host cells by injecting virulence-promoting effector proteins into the host cytosol. The T3SS is activated upon host cell contact, and its activation is accompanied by an arrest of cell division; hence, many species maintain a T3SS-inactive sibling population to propagate efficiently within the host. The...
Thu, 09/12/2019 - 14:14
Abstract In Mexico, there is limited information regarding food consumer behaviors at home. The knowledge of food consumption and food handling practices can help to determine more realistically the exposure to different hazards, such as Salmonella spp. on the population. The main goal of this study was to characterize the food consumption habits of five food groups (fruits and vegetables, fresh...
Thu, 09/12/2019 - 01:03
Available online 10 September 2019 Author(s): Meina Zhang, Yanyi Che, Shuang Zhao, Xia Xiaojing, Hongtao Liu, Jianfang Liu, Yuping Wang, Wenyu Han, Yuying Yang, Changhai Zhou, Liancheng LeiAbstractMastitis is a disease that affects dairy cattle and causes a decline in milk quality as well as economic loss worldwide. TGF-β1 levels are usually increased during mastitis; however, it is unknown...
Thu, 09/12/2019 - 01:03
LWT
Publication date: December 2019Source: LWT, Volume 116Author(s): Anuj Purohit, Anand MohanAbstractThis study was designed to assess the antimicrobial effect of pyruvic or succinic acid with oregano essential oil on Salmonella survival, composition of natural microflora, and resultant ground meat quality. Various concentrations of pyruvic (PA) and succinic acids (SA) with 0.5% essential oil (EO)...
Thu, 09/12/2019 - 00:00
Toxins, Vol. 11, Pages 528: Antibodies and Vaccines Against Botulinum Toxins: Available Measures and Novel Approaches Toxins doi: 10.3390/toxins11090528 Authors: Rasetti-Escargueil Popoff Botulinum neurotoxin (BoNT) is produced by the anaerobic, Gram-positive bacterium Clostridium botulinum. As one of the most poisonous toxins known and a potential bioterrosism agent, BoNT is...
Wed, 09/11/2019 - 20:00
Cattle have been identified as a major reservoir of E. coli O157:H7 for human infection; the ecology of the organism in sheep and goats is less understood. This study was carried out to determine prevalence, sour...
Wed, 09/11/2019 - 13:33
Abstract Reptile contact can result in zoonotic non‐typhoidal salmonellosis. In April 2018, Oregon Public Health Division contacted CDC about a cluster of four Salmonella serovar Fluntern (SF) illnesses in four states (OR, CA, IA, NY); patients reported contact with geckos, a popular reptile pet. PulseNet, the national molecular subtyping network of food‐borne disease surveillance, subsequently...
Wed, 09/11/2019 - 12:54
Abstract Aims The aim of this study was to develop a rapid detection and differentiation method for pathogenic Listeria species in stone fruits. Methods and Results We utilized activated charcoal enrichment media (ACM) to induce overexpression and hypersecretion of pathogenic Listeria virulence proteins which can subsequently be detected via immunoblot analysis. Plum and nectarine slices...
Wed, 09/11/2019 - 09:53
Abstract Detection of Salmonella on commercially processed broiler carcasses is essential to reduce the incidences of human salmonellosis. This study was designed to compare the standard procedure for Salmonella detection with two commercial screening methods. During seven separate visits to two processing plants, eight carcasses were obtained (n = 112). Each carcass was rinsed and the rinsate...
Wed, 09/11/2019 - 05:03
Abstract Aims To evaluate antibacterial properties of Methylsulfonylmethane (MSM) on Escherichia coli (MDRE21) and Salmonella enterica serovar Kinshasa (SK132). Methods and Results Bacterial proliferation analysis was measured spectrophotometrically during log phase growth with 0, 3, 5, 7, 10, 12 and 16% MSM. To assess the mechanism of inhibition, cultures were grown overnight with 0–16% MSM...
Wed, 09/11/2019 - 04:28
Abstract ESBL producing STEC are posing a constant threat to public health throughout the world leading to serious infections and raising key therapeutic issues. A total of 219 fecal samples were collected from piglets with diarrheoa, pig farmers, and water sources in North East India; and were processed for isolation of Escherichia coli. The isolates were screened for antimicrobial resistance...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Meera Surendran Nair, Fulin Ma, Patrick Lau, Indu Upadhyaya, Kumar VenkitanarayananAbstractThe study investigated the efficacy of two GRAS-status phytochemicals, mega-resveratrol (RV) and naringenin (NG) to inactivate Escherichia coli O157:H7 (EHEC) in apple cider. A five-strain mixture of EHEC (∼7 log CFU/ml) was inoculated into cider, followed by the...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Prabhat K. Talukdar, Mahfuzur R. SarkerAbstractClostridium perfringens SM101 genome encode three serine proteases (CspA, CspB, and CspC), and genetic evidence indicates that CspB is required for processing of pro-SleC into active SleC, an enzyme essential for degradation of the peptidoglycan cortex during spore germination. In this study, the...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Jiaming Huang, Bowen Chen, Huihui Li, Qiao-Hui Zeng, Jing Jing Wang, Haiquan Liu, Yingjie Pan, Yong ZhaoAbstractPhotodynamic inactivation (PDI) is a promising and effective non-thermal sterilization technology. This study aimed to construct the enhanced antibacterial and anti-biofilm blue light-emitting diode PDI with edible photosensitizer curcumin...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Jia Huang, Jiahui Sun, Antony R. Warden, Xianting DingAbstractWater-borne bacterial infection is a growing problem worldwide. Rapid and reliable contamination detection method for drinking-water is in high demand. Herein, a colorimetric bacteria detection strategy based on 4-mercaptophenylboronic acid (4-MPBA) functionalized gold nanoparticles (AuNPs)...
Wed, 09/11/2019 - 01:05
LWT
Publication date: Available online 8 September 2019Source: LWTAuthor(s): Jin-Hee Kim, Sun Jung, Se-Wook OhAbstractMicrobial enrichment of bacteria is essential for detection because generally foodborne pathogens exist on food at very low concentrations. This study described a rapid and simple pretreatment method, combination of bacteria concentration and DNA concentration for detection E. coli...
Wed, 09/11/2019 - 01:05
LWT
Publication date: Available online 8 September 2019Source: LWTAuthor(s): Stefani Machado Lopes, Eduardo César TondoAbstractPeruvian pisco sour is an alcoholic cocktail prepared with egg white in order to form a creamy texture. This drink has been served worldwide, however concerns about its safety have been raised because of the possibility of egg whites be contaminated by Salmonella. This study...
Wed, 09/11/2019 - 00:00
The traditional gold nanoparticle (AuNP) growth-based plasmonic ELISA (pELISA) strictly and directly controlled by reducing reagents can achieve high sensitivity, but it remains fragile toward the surrounding environment. This work developed a sandwich pELISA for Cronobacter detection in powdered infant formula samples by mediating AuNP growth through DNA. In this assay, DNA adsorbed on the...
Wed, 09/11/2019 - 00:00
Vibrio vulnificus is a Gram-negative naturally occurring bacterium found commonly in warm (>15oC), low-salinity/brackish waters worldwide. This bacterium occurs in high numbers in seafood, such as shellfish, particularly in warmer months. V. vulnificus is an important opportunistic pathogen that can cause both wound infections and sepsis, typically via exposure to seawater or through...
Wed, 09/11/2019 - 00:00
Abstract Probiotics are important bacteria due to their benefit on human health. In this study, four strains of lactic acid bacteria from chicken bile were isolated and the strain with the best antimicrobial activity was selected for further identification and evaluation on its probiotic traits and safety. The strain was identified as Enterococcus faecium by biochemical...
Wed, 09/11/2019 - 00:00
Toxins, Vol. 11, Pages 525: Vaccine Production to Protect Animals Against Pathogenic Clostridia Toxins doi: 10.3390/toxins11090525 Authors: Zaragoza Orellana Moonen Moutafis Marcellin Clostridium is a broad genus of anaerobic, spore-forming, rod-shaped, Gram-positive bacteria that can be found in different environments all around the world. The genus includes human and animal...
Tue, 09/10/2019 - 06:55
Abstract A method combining surface‐enhanced Raman scattering (SERS) with a lateral flow strip (LFS) was developed for the quantitative and sensitive analysis of Escherichia coli O157:H7. AuMBA@Ag nanoparticles were prepared as SERS probes, and 4‐methylthiobenzoic acid (MBA) as a Raman reporter was inserted into the interior gap of the Au@Ag core‐shell nanoparticles, which replaced the Au...
Mon, 09/09/2019 - 20:00
The Gram-negative bacterium Vibrio cholerae encodes two nucleases, Dns and Xds, which play a major role during the human pathogen’s lifecycle. Dns and Xds control three-dimensional biofilm formation and bacterial detachment from biofilms via degradation of extracellular DNA and thus contribute to the environmental, inter-epidemic persistence of the pathogen. During intestinal colonization the...
Mon, 09/09/2019 - 11:22
Macrophage parasitism by Coxiella burnetii, the cause of human Q fever, requires translocation of proteins with effector functions directly into the host cell cytosol via a Dot/Icm type 4B secretion system (T4BSS). Secretion by the analogous Legionella pneumophila T4BSS involves signal sequences within C-terminal and internal domains of effector proteins. The cytoplasmic chaperone pair IcmSW...
Mon, 09/09/2019 - 11:22
Production of flagella is costly and subject to global, multi-layered regulation. This is reflected in the hierarchical control of flagellar production in many bacterial species. For Salmonella enterica serovar Typhimurium and its relatives, global regulation of flagellar production primarily occurs through control of flhDC transcription and mRNA translation. In this study, the role of the...

Pages