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Bacterial pathogens

Tue, 03/12/2019 - 12:29
Abstract Ice is widely used to preserve fish on markets, playing a major role in the food industry. If manufactured, stored or distributed in inadequate sanitary conditions, it can represent a considerable health risk for both consumers and professionals. The present study characterizes ice used in marketplaces, on microbiological and chemical parameters. The aim was to assess potential risks on...
Tue, 03/12/2019 - 10:19
Abstract Fresh fruit is indispensable as a part of a daily diet; however, it commonly harbors large and diverse bacteria populations, including some important human pathogens. To determine the microbial composition on the surface of a strawberry, and structural changes of microbial composition from the field and market, 16SrDNA sequencing of 32 samples collected from production field (B) and...
Tue, 03/12/2019 - 04:42
Abstract Salmonella is one of the most common foodborne pathogens worldwide. Salmonella infections in humans are mainly associated with consumption of poultry products contaminated with this foodborne pathogen. Therefore, strict sanitary measures are necessary to control Salmonella contamination during the slaughtering process of poultry. The objective of this study was to determine the...
Tue, 03/12/2019 - 01:14
Available online 11 March 2019 Author(s): Sun-Ah Kwon, Won-Jae Song, Dong-Hyun KangAbstractThe purpose of this study was to evaluate the effectiveness of the combination treatment of lactic acid immersion and saturated or superheated steam (SHS) on inactivation of foodborne pathogens on cantaloupes. Saturated steam (SS) treatments were performed at 100°C, while SHS treatments were delivered at...
Tue, 03/12/2019 - 01:14
Available online 11 March 2019 Author(s): Sang-Soon Kim, Sang-Hyun Park, Soo-Hwan Kim, Dong-Hyun KangAbstractHurdle technology, in which preservation techniques are combined, has been of interest to scientists aiming to ensure microbiological food safety. We investigated the synergistic effect of UV-C irradiation and ohmic heating on the inactivation of foodborne pathogens. UV-C irradiation and...
Mon, 03/11/2019 - 17:00
Neha Dabral, Grant N. Burcham, Neeta Jain-Gupta, Nammalwar Sriranganathan, Ramesh Vemulapalli Brucella abortus RB51 is an attenuated, stable, spontaneous rough mutant derived in the laboratory from the virulent strain B. abortus 2308. Previous studies discovered that the wboA gene, which encodes a glycosyltransferase required for synthesis of the O-polysaccharide, is disrupted in strain RB51 an...
Mon, 03/11/2019 - 12:10
Abstract Endemic zoonoses, such as Q fever and spotted fever group (SFG) rickettsiosis, are prevalent in South Africa, yet often undiagnosed. In this study, we reviewed the demographics and animal exposure history of patients presenting with acute febrile illness to community health clinics in Mpumalanga Province to identify trends and risk factors associated with exposure to Coxiella burnetii,...
Mon, 03/11/2019 - 11:32
The phage shock protein (Psp) system is a stress response pathway that senses and responds to inner membrane damage. The genetic components of the Psp system are present in several clinically relevant Gram-negative bacteria, including Vibrio cholerae. However, most of the current knowledge about the Psp response stems from in vitro studies in Escherichia coli and Yersinia enterocolitica. In fact...
Mon, 03/11/2019 - 11:32
Conjugative plasmids of the IncC group, formerly known as A/C2, disseminate antibiotic resistance genes globally in diverse pathogenic species of Gammaproteobacteria. Salmonella genomic island 1 (SGI1) can be mobilized by IncC plasmids and was recently shown to reshape the conjugative type IV secretion system (T4SS) encoded by these plasmids to evade entry exclusion. Entry exclusion blocks DNA...
Mon, 03/11/2019 - 11:32
Korormicin is an antibiotic, produced by some Pseudoalteromonads, which selectively kills gram negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that, although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Instead, perturbation of electron transfer inside the enzyme promotes a reaction...
Mon, 03/11/2019 - 01:11
Available online 9 March 2019 Author(s): Xinyao Wei, Soon Kiat Lau, Jayne Stratton, Sibel Irmak, Jeyamkondan SubbiahAbstractSalmonella persistence in ground black pepper has caused several foodborne outbreaks and created public concern about the safety of low water activity (aw) foods. In this study, radiofrequency (RF) processing was evaluated for pasteurization of ground black pepper. Stability...
Mon, 03/11/2019 - 00:00
Abstract Blue cheeses are susceptible to yeast and bacterial growth on their surface, which causes spoilage during ripening process and the formation of slime. The dairy industry frequently control the proliferation of undesirable microorganisms with natamycin and high salt concentration. The green propolis is a complex of substances that presents antimicrobial properties with...
Sun, 03/10/2019 - 01:14
Publication date: Available online 8 March 2019Source: LWTAuthor(s): Duo Liang, Qingfa Wang, Dandan Zhao, Xue Han, Jianxiong HaoAbstractThe systematic application of slightly acidic electrolyzed water (SAEW) for natural microbial reduction of buckwheat sprouts were investigated including seed-dipping, germination and sprouts-washing in the present study. The results show that all the three SAEW...
Sun, 03/10/2019 - 01:14
Available online 8 March 2019 Author(s): Hang Qi, Qingguo Huang, Yen-Con HungAbstractActivated persulfate, a relatively new advanced oxidation process, has gained attention for its potential to ensure fresh produce safety. One of the major advantages is to avoid the formation of toxic chlorinated disinfection byproducts which are concerns for chlorine-based sanitizers. This study was aimed to...
Sun, 03/10/2019 - 01:13
Available online 9 March 2019 Author(s): Reinhard Sting, Tobias Eisenberg, Maja HrubenjaAbstractAnalyses of short subunit gene sequences have been established for taxonomic classification and identification of bacteria and fungi. To produce partial bacterial ribosomal 16S rRNA and rpoB and fungal ribosomal ITS/LSU gene sequences for DNA sequencing, real-time PCR assays supplemented with the...
Sun, 03/10/2019 - 01:13
Available online 8 March 2019 Author(s): Adrián Gómez-Baltazar, Ma Soledad Vázquez-Garcidueñas, John Larsen, Mariana Esther Kuk-Soberanis, Gerardo Vázquez-MarrufoAbstractThe replacement of the most prevalent Salmonella enterica genotypes has been documented worldwide. Here we tested the hypothesis that the current prevalent sequence type ST213 of serotype Typhimurium in Mexico has a higher...
Sun, 03/10/2019 - 01:13
Available online 8 March 2019 Author(s): Thea King, Cassandra J. Vockler, Theo R. Allnutt, Narelle FeganAbstractThe majority of foodborne illnesses associated with E. coli O157 are attributed to the consumption of foods of bovine origin. In this study, RNA-Seq experiments were undertaken with E. coli O157 to identify genes that may be associated with growth and survival on meat and the beef...
Sat, 03/09/2019 - 01:12
Publication date: Available online 7 March 2019Source: LWTAuthor(s): Clarissa Barretta, Silvani Verruck, Bruna Marchesan Maran, Leticia dos Santos Maurício, Marília Miotto, Cleide Rosana Werneck Vieira, Elane Schwinden PrudencioAbstractBecause the food matrix is considered as one of the significant factors in regulating colonization of microorganisms in the gastrointestinal tract, this study...
Sat, 03/09/2019 - 01:12
Publication date: Available online 7 March 2019Source: LWTAuthor(s): Fabiane Bach, Acácio Antonio Ferreira Zielinski, Cristiane Vieira Helm, Giselle Maria Maciel, Alessandra Cristina Pedro, Ana Paula Stafussa, Suelen Ávila, Charles Windson Isidoro HaminiukAbstractThe aim of this study was to optimize the extraction of total phenolics from edible mushrooms, evaluate the in vitro antioxidant and...
Sat, 03/09/2019 - 00:00
Abstract In order to prolong the shelf life of Chinese traditional dry-cured meat products, a pulsed ultraviolet light (PL-UV) irradiation method was adopted to treat meat products according to the following parameters: pulse energy of 8 J, 300 pulses, and an effective exposure distance of 10 cm; the UV light irradiation power of 6 W, an effective exposure distance of 11 cm, and...
Fri, 03/08/2019 - 17:00
Reda Mohamed-Salem, Carmina Rodríguez Fernández, Elvira Nieto-Pelegrín, Beatriz Conde-Valentín, Angel Rumbero, Narcisa Martinez-Quiles Diarrheic diseases account for the annual death of approximately 1.9 million children under the age of 5 years, and it is a major cause of work absenteeism in developed countries. As diarrheagenic bacteria, enteropathogenic Escherichia coli (EPEC) attach to cells...
Fri, 03/08/2019 - 07:54
Significance and Impact of the Study: Alkaloids are abundant secondary metabolites in plants and represent one of the most widespread class of compounds endowed with multiple and varied pharmacological properties. In this work, we propose two aporphinoid alkaloids extracted from plants as new antimicrobial agents. Oliveridine and pachypodanthine inhibited Yersinia enterocolitica growth for up to...
Fri, 03/08/2019 - 01:14
The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in Yersinia pseudotuberculosis induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content...
Fri, 03/08/2019 - 01:14
Available online 6 March 2019 Author(s): María Cevallos-Almeida, Laure Martin, Catherine Houdayer, Valerie Rose, Jean-Marie Guionnet, Frédéric Paboeuf, Martine Denis, Annaëlle KerouantonAbstractSalmonella serovars Derby, Typhimurium and the monophasic variant of Salmonella Typhimurium are the most frequently isolated serovars in pigs in France. To compare the excretion patterns, seroconversion to...
Fri, 03/08/2019 - 01:14
Available online 6 March 2019 Author(s): Chunhua Wu, Yang Zhu, Tiantian Wu, Lin Wang, Yi Yuan, Jicheng Chen, Yaqin Hu, Jie PangAbstractCurcumin loaded mesoporous silica nanoparticle (SBA-15) was incorporated into chitosan (CS) film to improve the functional properties of pure CS film. Curcumin was loaded into SBA-15 (SBA-15-Cur) through a rotavapor method. The structural properties of SBA-15-Cur...
Fri, 03/08/2019 - 01:13
Available online 6 March 2019 Author(s): Jean Carlos Correia Peres Costa, Sara Bover-Cid, Araceli Bolívar, Gonzalo Zurera, Fernando Pérez-RodríguezAbstractThe objective of this work was to quantitatively evaluate the effect of Lactobacillus sakei CTC494 (sakacin-producing bioprotective strain) against Listeria monocytogenes in fish juice and to apply and validate three microbial interaction...
Fri, 03/08/2019 - 01:13
Available online 6 March 2019 Author(s): Antonio Parisi, Marta Caruso, Giovanni Normanno, Laura Latorre, Angela Miccolupo, Rosa Fraccalvieri, Francesco Intini, Teresa Manginelli, Gianfranco SantagadaAbstractMethicillin-resistant Staphylococcus aureus (MRSA) is an important medical issue, since it causes serious and sometimes fatal infections in humans. Intensively reared swine may serve as...
Fri, 03/08/2019 - 01:13
Publication date: Available online 6 March 2019Source: LWTAuthor(s): Piyanan Chuesiang, Ubonrat Siripatrawan, Romanee Sanguandeekul, Jason Szuhao Yang, David Julian McClements, Lynne McLandsboroughAbstractThe water-dispersibility and antimicrobial activity of cinnamon oil is enhanced by encapsulating it within oil-in-water nanoemulsions. This study investigated the impact of oil phase composition...
Fri, 03/08/2019 - 01:13
Publication date: Available online 5 March 2019Source: LWTAuthor(s): Danielle Oliveira Borges, Mariana Mitiko Matsuo, Cristina S.Bittencourt Bogsan, Tatiane Ferreira da Silva, Sabrina Neves Casarotti, Ana Lúcia Barretto PennaAbstractThe effect of Ln. mesenteroides subsp. mesenteroids SJRP55 on the fatty acid profile, on content of bioactive unsaturated fatty acids in fermented cream, ability to...
Thu, 03/07/2019 - 17:00
Jae Seung Yang, So Jung An, Mi Seon Jang, Manki Song, Seung Hyun Han Serum vibriocidal antibody assays have long been used to evaluate the immunogenicity of cholera vaccines formulated with killed whole-cell Vibrio cholerae. However, the antibody isotypes responsible for the serum vibriocidal activity are not fully characterized. In this study, we examined 20 clinical serum samples obtained from...
Thu, 03/07/2019 - 08:30
Sensorially accepted concentrations of MPEO were evaluated as a single agent and in combination with MHT to cause a 5 log10 reduction in survival counts of E. coli O157:H7 and S. Enteritidis PT4 without affecting the physicochemical parameters of the caja, guava and mango juices. Summary This study determined the compromised acceptance threshold (CAT) and rejection threshold (RT) of Mentha...
Thu, 03/07/2019 - 01:16
Available online 1 March 2019 Author(s): Fernando L. Leite, Erika Vasquez, Connie J. Gebhart, Richard E. IsaacsonAbstractLawsonia intracellularis is among the most important enteric pathogens of swine and has been shown to be a risk factor for increased Salmonella enterica shedding. S. enterica serovar Typhimurium, in addition to being a significant pathogen of swine, also remains one of the most...
Thu, 03/07/2019 - 01:14
Publication date: 30 July 2019 , Volume 287Author(s): Ana Gluvić, Nataša Poklar UlrihAbstractWe studied seven known peptides derived from different food proteins, and one hydrophobic peptide derived through in-silico hydrolysis. We investigated their antioxidative, antimicrobial and haemolytic activities and their interactions with model lipid membranes. Our data reveal that peptides with...
Thu, 03/07/2019 - 01:14
Publication date: 30 July 2019 , Volume 287Author(s): Qingyang Wang, Wai Fun Leong, Ryan J. Elias, Rohan V. TikekarAbstractA novel light-enhanced antimicrobial treatment was developed by exposing gallic acid (GA) to UV-C light. GA (15 mM) solution was exposed to UV-C for 30 min and subsequently incubated with E. coli O157:H7 for 30 min to achieve a 3.2 ± 0.2 log CFU/mL reduction. The...
Thu, 03/07/2019 - 01:14
Publication date: 30 July 2019 , Volume 287Author(s): Branislav Šojić, Branimir Pavlić, Vladimir Tomović, Predrag Ikonić, Zoran Zeković, Sunčica Kocić-Tanackov, Saša Đurović, Snežana Škaljac, Marija Jokanović, Maja IvićAbstractThe effect of winter savory (Satureja montana L.) essential oil (SEO) and supercritical extracts (SE1; SE2) at concentrations of 0.075 and 0.150 µL/g on pH, lipid oxidation...
Thu, 03/07/2019 - 01:14
Available online 5 March 2019 Author(s): Lin Zhao, Mitchie Yiqi Zhao, Chin Ping Phey, Hongshun YangAbstractThe sanitising effect of low concentration acidic electrolysed water (AEW, free available chlorine (FAC): 4 mg/L) combined with levulinic acid (LA, 3% v/v) on fresh organic lettuce during 7-day storage was evaluated. The combined sanitising method showed additional bactericidal efficacy...
Wed, 03/06/2019 - 11:00
Klebsiella pneumoniae is a leading cause of severe infections in humans and dairy cows, and these infections are rapidly becoming untreatable due to the emergence of multidrug-resistant (MDR) strains. However, little is known about the relationship between bovine and human K. pneumoniae isolates at the genome population level. Here, we investigated the genomic structures, pangenomic profiles,...
Wed, 03/06/2019 - 11:00
Atypical enteropathogenic Escherichia coli (aEPEC) strains (36 Japanese and 50 Bangladeshi) obtained from 649 poultry fecal samples were analyzed by molecular epidemiological methods. Clermont’s phylogenetic typing showed that group A was more prevalent (58%, 50/86) than B1 (31%, 27/86). Intimin type β1, which is prevalent among human diarrheal patients, was predominant in both...
Wed, 03/06/2019 - 11:00
We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amplification products are simply loaded on a standard agarose gel for electrophoresis, and the...
Wed, 03/06/2019 - 11:00
Efficient microbial conversion of lignocellulose into valuable products is often hindered by the presence of furfural, a dehydration product of pentoses in hemicellulose sugar syrups derived from woody biomass. For a cost-effective lignocellulose microbial conversion, robust biocatalysts are needed that can tolerate toxic inhibitors while maintaining optimal metabolic activities. A comprehensive...
Wed, 03/06/2019 - 11:00
In this study, we examined the change in resistance of Salmonella enterica serovar Typhimurium and Escherichia coli O157:H7 to 222-nm krypton-chlorine (KrCl) excilamp treatment as influenced by acid adaptation and identified a mechanism of resistance change. In addition, we measured changes in apple juice quality indicators, such as color, total phenols, and 2,2-diphenyl-1-picrylhydrazyl (DPPH)...
Wed, 03/06/2019 - 08:39
Abstract CTX‐M‐type extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae have become identified in marine ecosystem constituting a serious ecological issue. In this respect, although contamination of coastal waters and seafood, and even colonization of seabirds and fishes have been increasingly reported, molecular data are lacking to elucidate the clinical impact of ESBL producers in...
Wed, 03/06/2019 - 01:04
Nikola Popović, Jelena Djokić, Emilija Brdarić, Miroslav Dinić, Amarela Terzić-Vidojević, Nataša Golić, Katarina Veljović
Wed, 03/06/2019 - 01:04
Publication date: Available online 3 March 2019Source: LWTAuthor(s): Marilyn C. Erickson, Jye-Yin LiaoAbstractThis study investigated the degree of extraction from leafy green tissue (green leafy lettuce, spinach, red cabbage) artificially inoculated with five individual strains of Salmonella at 3–4 log CFU. Inclusion of salt at levels of 0.50 M–0.75 M in the extraction solution improved recovery...
Wed, 03/06/2019 - 00:00
Intoxication by staphylococcal enterotoxins (SE) is among the most common causes of food-poisoning outbreaks resulting from the consumption of raw milk or products made thereof. The aim of our study was to analyze the thermal stability of SE and evaluate the inactivation of SE types A, B, and C (SEA, SEB, SEC) by autoclaving at 100°C, 110°C, and 121°C. Milk samples were inoculated with 38...

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