Bacterial pathogens

Fri, 08/14/2020 - 06:13
Author(s): Yukiko Hara-Kudo, Kayoko Ohtsuka, Noriko Konishi, Takako Yoshida, Kaori Iwabuchi, Takahiro Hiratsuka, Yuhki Nagai, Keiko Kimata, Hiroyuki Wada, Takumiko Yamazaki, Akihiko Tsuchiya, Tetsuya Mori, Shunichi Inagaki, Shogo Shiraishi, Jun Terajima Enterotoxigenic Escherichia coli (ETEC) causes acute diarrhea and is transmitted through contaminated food and water; however, systematic...
Fri, 08/14/2020 - 06:12
Author(s): Yilan Li, Qiuping Yang, Chenyang Cao, Shenghui Cui, Yun Wu, Hua Yang, Yingping Xiao, Baowei Yang In this study, we evaluated the prevalence of Salmonella in retail raw chickens in Shaanxi Province, China on a monthly basis. Additionally, we studied the antibiotic susceptibility, serotype, and genotype of Salmonella isolates and explored their relationships with sampling time, location...
Fri, 08/14/2020 - 02:03
Multiple studies have demonstrated atmospheric cold plasma as an effective non-thermal technology for inactivating bacteria, spores, and other microbial contaminants in foods and on non-food surfaces. However, few studies have applied this technique to semi-solid food within a package. This study evaluates the efficacy and the interaction mechanism of high voltage atmospheric cold plasma (HVACP)...
Fri, 08/14/2020 - 02:02
Wastewater treatment plants (WWTPs), usually designed to remove organic pollutants and nutrients, are often poorly equipped to handle pathogens. The present study investigated the multiple barriers provided by WWTPs to understand their role in spreading pathogenic bacteria into the environment. Three types of WWTPs (hospital, domestic, and mixed) differing in the source of raw influent, operating...
Fri, 08/14/2020 - 00:00
Carbapenem resistant Klebsiella pneumoniae (CRKP), Pseudomonas aeruginosa (CRPA), and Acinetobacter baumannii (CRAB) pose significant threats to public health. However, the clinical and economic impacts of CRKP, CRPA, and CRAB remain largely uninvestigated in China. This study aimed to examine the clinical and economic burden of CRKP, CRPA, and CRAB compared with carbapenem susceptible cases in...
Fri, 08/14/2020 - 00:00
Tricholosporum goniospermum (Bres.) Guzmán ex T.J. Baroni is an excellent edible mushroom whose compounds and biological properties are still unknown. In this study, n-hexane, ethyl acetate and methanol extracts from fruiting bodies and liquid-cultured mycelia were compared for the analysis of phenolic compounds, the evaluation of scavenger (DPPH, ABTS) and reducing (CUPRAC, FRAP)...
Thu, 08/13/2020 - 22:19
Herein, Psyllium‐seed mucilage (PSM) and oregano extract (OE) were incorporated into the design of a promising, biodegradable, and antimicrobial edible film to enhance the shelf life of food. Various OE‐PSM films with different concentrations of OE, PSM, and glycerol, as a plasticizer, were fabricated. Further physicochemical, mechanical, and structural studies demonstrated an enhancement in the...
Thu, 08/13/2020 - 22:19
A new selective broth, SSEL, was developed for simultaneous enrichment of S. enterica, S. aureus, E. coli O157: H7, and L. monocytogenes, optimizing by single factor experiments and a L9 (34) orthogonal test. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully...
Thu, 08/13/2020 - 22:18
The impact of high‐pressure processing (HPP) or thermosonication (TS) in combination with nisin on the microbial inactivation of green juice (celery stalk, apple, cucumber, parsley) and changes in its quality was investigated. Microbial inactivation with HPP at 600 MPa resulted ≥7 log reduction of Escherichia coli, whereas 5 and 7 log reduction of Listeria innocua were attained after combining...
Thu, 08/13/2020 - 22:18
The objective of this study was to compare the impact of ultrasound (US; 30 kHz; 2, 4, 6, and 8 min) and malic acid (MA; 0.5 and 1%), individually and in combination, on inactivation of food borne pathogens (Salmonella Typhi, Shigella dysenteriae, Listeria monocytogenes and Shigella flexneri) and the effect of US treatment on β‐carotene and total phenolics content in sweet lemon juice. The extent...
Thu, 08/13/2020 - 22:14
Antigen‐specific CD8+ T‐lymphocytes (cytotoxic T‐lymphocytes: CTL), as well as CD4+ T‐lymphocytes (helper T‐lymphocytes: Th), simultaneously play an important role in elimination of intracellular bacteria such as Mycobacterium tuberculosis and Listeria monocytogenes. Administration of T‐cell epitope short peptide needs large numbers of peptides for effective vaccination due to its easily...
Thu, 08/13/2020 - 22:13
Background Combination of high pressure processing (HPP) and antimicrobials is a well‐known approach to enhance the microbiological safety of foods. However, few researchers have applied multiple antimicrobials simultaneously with HPP, which could be an additional hurdle for microbial inactivation. This study applied a full factorial design to investigate the impact of HPP (225‐325 MPa; 10‐20 min...
Thu, 08/13/2020 - 16:00
Bacteria belonging to Staphylococcus genus, in particular methicillin-resistant Staphylococcus aureus and multidrug-resistant Staphylococcus epidermidis, together with Cutibacterium acnes are the main strains involved in skin disease. The increase in multidrug-resistant bacteria has revived attention on natural compounds as alternative agents for the treatment management. Among these, hop extract...
Thu, 08/13/2020 - 16:00
Rapid developments in the field of whole genome sequencing (WGS) make in silico antimicrobial resistance (AMR) a target within reach. Campylobacter jejuni is a leading cause of foodborne infections in Israel with increasing rates of resistance. We applied WGS analysis to study the prevalence and genetic basis of AMR in 263 C. jejuni human and veterinary representative isolates retrieved from a...
Thu, 08/13/2020 - 16:00
Coxiella burnetii is an obligate intracellular Gram-negative bacterium that causes a zoonotic disease commonly called Q fever globally. In this study, an up-converting phosphor technology-based lateral flow (UPT-...
Thu, 08/13/2020 - 10:03
ABSTRACT To understand toxin-stimulated host-pathogen interactions, we performed dual-transcriptome sequencing experiments using human epithelial (HT-29) and differentiated THP-1 (dTHP-1) immune cells infected with the sepsis-causing pathogen Vibrio vulnificus (either the wild-type [WT] pathogen or a multifunctional-autoprocessing repeats-in-toxin [MARTX] toxin-deficient strain). Gene set...
Thu, 08/13/2020 - 10:03
We report the chromosome and plasmid sequences of a strain of Listeria monocytogenes IVb variant 1, a recently emerging serotype, isolated in Italy from ready-to-eat vegetables.
Thu, 08/13/2020 - 06:24
Author(s): C. Rodriguez, B. Taminiau, E. García-Fuentes, G. Daube, N. Korsak Listeria monocytogenes (L. monocytogenes) is a well-known pathogenic bacterium that causes the disease listeriosis in both humans and animals. Human transmission mainly occurs via ingestion of contaminated foods and specifically affects pregnant women, newborns, elderly individuals and immunosuppressed individuals....
Thu, 08/13/2020 - 06:19
Author(s): Arícia Possas, Fernando Pérez-Rodríguez, Fatih Tarlak, Rosa Maria García-Gimeno The efficacy of electrolyzed water (EW) to inactivate Listeria monocytogenes on stainless steel surfaces was evaluated and modelled in the present study. L. monocytogenes was inoculated on stainless steel coupons and subsequently subjected to Neutral EW (NEW, pH = 7.0) and Slightly Acid EW (SAEW, pH = 5.0)...
Thu, 08/13/2020 - 06:17
LWT
Author(s): Gulten Izmirlioglu, Beining Ouyang, Ali Demirci Due to their nutritional value, walnut consumption has been increasing worldwide, however outbreaks caused by walnuts are still of concern. Current chemical methods in walnut industry to prevent pathogenic microorganisms are not preferred by the consumer while thermal treatments may cause undesired flavor. UV light is the portion of the...
Thu, 08/13/2020 - 06:17
LWT
Author(s): Emmanuel O. Kyere, Goh Wan Qiu, Siti Norbaizura Md Zain, Jon Palmer, Jason J. Wargent, Graham C. Fletcher, Steve Flint The popularity of bagged salads sold in the market has increased due to their ease of transportation and convenience but there are food safety risks. In this study, we examined the prevalence of Listeria monocytogenes (L. monocytogenes) in 100 samples of ready-to-eat...
Wed, 08/12/2020 - 22:20
Antimicrobials are the most important therapy to bovine mastitis. Bacterial infection and antibiotic treatment of mastitis cycles frequently in dairy farms worldwide, giving rise to concerns about the emergence of multidrug‐resistant (MDR) bacteria. In this study, we examined the microbial diversity and antibiotic resistance profiles of bacteria isolated from raw milk from dairy farms in Jiangsu...
Wed, 08/12/2020 - 22:20
Escherichia coli O157:H7, a causative agent of haemolytic uremic syndrome, can enter into a viable but non‐culturable (VBNC) state in response to harsh stress. Bacteria in this state can retain membrane integrity, metabolic activity and virulence expression, which may present health risks. However, virulence expression and resuscitation ability of the VBNC state are not well understood. Here, we...
Wed, 08/12/2020 - 22:16
As filter‐feeders, bivalve molluscs accumulate Vibrio into edible tissues. Consequently, an accurate assessment of depuration procedures and the characterization of the persistent Vibrio community in depurated shellfish represent a key issue to guarantee food safety in shellfish products. The present study investigated changes in the natural Vibrio community composition of the Ruditapes...
Wed, 08/12/2020 - 22:15
Aims Characterization of quinolone‐resistant Salmonella Kentucky and Typhimurium isolates in Tunisia from various sources, detection of some PMQR genes, and the genetic relatedness. Methods A total of 1,404 isolates of S. Kentucky (n = 1,059) / S. Typhimurium (n = 345) from various sources from all over Tunisia were tested for quinolone resistance by disk diffusion method. Minimum inhibitory...
Wed, 08/12/2020 - 16:00
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are diverse immune systems found in many prokaryotic genomes that target invading foreign DNA such as bacteriophages and plasmids. There are multiple types of CRISPR with arguably the most enigmatic being Type IV. During an investigation of CRISPR carriage in clinical, multi-drug resistant, Klebsiella pneumoniae, a Type IV-A3...
Wed, 08/12/2020 - 16:00
Vibrio parahaemolyticus is a significant seafood-borne pathogen, leading to serious acute gastrointestinal diseases worldwide. In this study, a reliable 4-plex droplet digital PCR (ddPCR) was successfully established and evaluated for the simultaneous detection of V. parahaemolyticus based on tlh, tdh, ureR, and orf8 in food samples using single intact cells. The targets tlh and ureR were labeled...
Wed, 08/12/2020 - 16:00
At present, azithromycin has become an effective treatment for severe diarrhea caused by Enterotoxigenic Escherichia coli (ETEC) infection. However, enterobacteria have begun to develop resistance to azithromycin...
Wed, 08/12/2020 - 00:00
Antimicrobial resistance (AMR) is one of the most prominent public health threats. AMR genes localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for the detection of AMR genes; however, reliable reconstruction of plasmids is still a challenge due...
Tue, 08/11/2020 - 22:17
Isochoric freezing, different from isobaric (conventional) freezing, has a superior capability to reduce foodborne pathogens (a) photo and (b) schematic of a prototypical isochoric chamber. Abstract Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation. While several types of tissues have...
Tue, 08/11/2020 - 16:00
The family Tephritidae includes some of the most notorious insect pests of agricultural and horticultural crops in tropical and sub-tropical regions. Despite the interest in the study of their gut microbiome, our present knowledge is largely based on the analysis of laboratory strains. In this study, we present a first comparative analysis of the gut microbiome profiles of field populations of...
Tue, 08/11/2020 - 16:00
After first detections of carbapenemase-producing Enterobacteriaceae (CPE) in animals, the European Union Reference Laboratory for Antimicrobial Resistance has provided a protocol for the isolation of carbapenemase-producing Escherichia (E.) coli from cecum content and meat. Up to now, only few isolates were recovered using this procedure. In our experience, the choice of the selective agar is...
Tue, 08/11/2020 - 12:00
ABSTRACT This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, 99 water samples from 33 lots (n = 3/batch) of 15 brands, obtained from packs with three intervals of Tm, were analyzed. Total...
Tue, 08/11/2020 - 12:00
ABSTRACT The emergence of plasmid-mediated colistin resistance genes (mcr), which is occurring in numerous countries, is a worldwide concern, primarily because colistin is a last-resort antibiotic. Compared to E. coli, prevalence of mcr genes in Salmonella is unclear in Japan. Here we screened for mcr-1–5 genes in our collection of Salmonella strains isolated from retail meat products collected...
Tue, 08/11/2020 - 10:03
Uropathogenic Escherichia coli (UPEC) are the leading cause of human urinary tract infections (UTIs) and many patients experience recurrent infection after successful antibiotic treatment. The source of recurrent infections may be persistent bacterial reservoirs in vivo that are in a quiescent state and thus are not susceptible to antibiotics. Here, we show that multiple UPEC strains require a...
Tue, 08/11/2020 - 10:03
Cohesion of biofilms made by Yersinia pestis and Yersinia pseudotuberculosis (Yptb) has been attributed solely to an extracellular polysaccharide matrix encoded by the hms genes (Hms-ECM). However, mutations in the Yptb BarA/UvrY/CsrB regulatory cascade enhance biofilm stability without dramatically increasing Hms-ECM production. We found that treatment with proteinase K enzyme effectively...
Tue, 08/11/2020 - 06:29
Author(s): Hiroshi Ashida, Toshihiko Suzuki, Chihiro Sasakawa In response to bacterial infection, epithelial cells undergo several types of cell death, including apoptosis, necrosis, pyroptosis, and necroptosis, which serve to expel the infected cells and activate the innate and acquired immune responses. Shigella initially invades macrophages and subsequently surrounding enterocytes; the...
Tue, 08/11/2020 - 06:25
Author(s): Dayna M. Harhay, Margaret D. Weinroth, James L. Bono, Gregory P. Harhay, Joseph M. Bosilevac Little progress has been made in decreasing the incidence rate of salmonellosis in the US over the past decade. Mitigating the contribution of contaminated raw meat to the salmonellosis incidence rate requires rapid methods for quantifying Salmonella, so that highly contaminated products can be...
Tue, 08/11/2020 - 06:25
Author(s): Vijay K. Juneja, Anuj S. Purohit, Max Golden, Marangeli Osoria, Kathleen A. Glass, Abhinav Mishra, Harshavardhan Thippareddi, Govindaraj Devkumar, Tim B. Mohr, Udit Minocha, Meryl Silverman, Donald W. Schaffner A dynamic model to predict the germination and outgrowth of Clostridium botulinum spores in cooked ground beef was presented. Raw ground beef was inoculated with a ten-strain C...
Mon, 08/10/2020 - 22:15
Research on the initial phage‐host interaction has been conducted on a limited repertoire of phages and their cognate receptors, such as phage λ and the Escherichia coli LamB (EcLamB) protein. Apart from phage λ, little is known about other phages which target EcLamB. Here, we developed a simple method for isolating novel environmental phages in a predictable way, i.e. isolating phages which...
Mon, 08/10/2020 - 06:13
Author(s): Jennifer C. Acuff, Jian Wu, Claire Marik, Kim Waterman, Daniel Gallagher, Haibo Huang, Robert C. Williams, Monica A. Ponder Salmonella, Shiga toxin-producing Escherichia coli (STEC), and Listeria monocytogenes have been isolated from low water activity foods (LWAF), where they may survive for extended periods. The ready-to-eat nature of many LWAF, such as dried fruits and nuts,...
Mon, 08/10/2020 - 06:13
Author(s): E. Tirloni, M. Nauta, M. Vasconi, V. Di Pietro, C. Bernardi, S. Stella The present study investigated the presence, growth potential, and public health risk posed by Listeria monocytogenes in a ready-to-eat “shrimp cocktail”. The pathogen was detected in 4 out of the 104 samples, and there were no counts above the enumeration limit (1 Log colony-forming unit (CFU)/g); the product was a...
Sun, 08/09/2020 - 06:16
Author(s): Chang-Hong Cheng, Hong-Ling Ma, Yi-Qin Deng, Juan Feng, Yu-Ken Jie, Zhi-Xun Guo Mud crab (Scylla paramamosain) is an important economic species in China. Vibrio parahaemolyticus infection have caused a great economic loss in mud crab farming. The mechanism involved in the immune responses of mud crab to V. parahaemolyticus is unclear. In this study, the physiological and immune...
Sun, 08/09/2020 - 06:16
Author(s): Soheil Yousefi, Maryam Monsef Shokri, Hamid Allaf Noveirian, Seyed Hossein Hoseinifar The present study aims to shed light on the effects of yeast cell wall (ImmunoWall®) supplementation on biochemical indices, oxidative status, serum and mucus immune responses as well as disease resistance of juvenile Persian sturgeon (Acipenser persicus). For this purpose, one hundred fifty three...
Sun, 08/09/2020 - 06:15
Author(s): Esben Bragason, Tesfemariam Berhe, Dakalo Dashe, Kim Ib Sørensen, Mituku E. Guya, Egon Bech Hansen Bad hygiene and lack of cooling facilities have resulted in spontaneously fermented African camel milk with high incidence of contaminants. Starter cultures promote food safety through fermentation control. Commercial cultures developed for bovine milk acidify poorly in camel milk and...
Sun, 08/09/2020 - 06:15
Author(s): Ru Zhang, Linshu Lan, Hui Shi Freezing treatment is widely used to extend the shelf life of food, but sublethally injured Escherichia coli O157:H7 caused by freezing and thawing is a potential threat in food safety. This study investigated the sublethal injury of E. coli O157:H7 (NCTC 12900) after exposure to different freezing treatments (freezing time: 4 h, 8 h, 12 h and 16 h,...
Sun, 08/09/2020 - 06:15
Author(s): Mi-Ju Kim, Hyun-Joong Kim, Hae-Yeong Kim In this study, a novel triplex loop-mediated isothermal amplification (LAMP) assay was developed and applied to a model of representative foodborne pathogen, Salmonella, to investigate the performance of the assay with high rapidity, specificity and sensitivity, and its ability to detect a pathogen in contaminated food. Three sets of LAMP...
Sun, 08/09/2020 - 06:12
Author(s): Mashael R. Aljumaah, Gamaleldein M. Suliman, Abdulaziz Alabdullatif, Alaeldein, M. Abudabos Because of concerns over the use of antibiotics in poultry feed, this study was designed to determine the effectiveness of phytobiotic supplementation as an alternative to antibiotic use based on growth performance and meat characteristics of broilers exposed to Salmonella typhimurium. The...
Sun, 08/09/2020 - 06:12
Author(s): Dongryeoul Bae, Dong-Hyeon Kim, Jung-Whan Chon, Kwang-Young Song, Kun-Ho Seo In this study, we aimed to assess the feasibility of the lactic acid bacterium Lactobacillus kefiranofaciens DN1 (LKF_DN1) and the yeast Kluyveromyces marxianus KU140723-05 (KMA5), recently isolated from kefir, as probiotics. Specifically, we evaluated the effect of early administration of these two microbes...
Sat, 08/08/2020 - 22:20
Numerous infectious diseases impacting livestock impose an important economic burden, and in some cases also represent a threat to humans and are classified as zoonoses. Some zoonotic diseases are transmitted by vectors and, due to complex environmental and socio‐ecconomic factors, the distribution of many of these pathogens is changing, with increasing numbers being found in previously...

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