Bacterial pathogens

Wed, 12/09/2020 - 00:00
Methicillin-resistant Staphylococcus spp. (MRS) have been identified in several foods, including dairy products. Studies are needed about their occurrence and genetic diversity in the dairy production chain in order to gain a better understanding of their epidemiology and control. This study therefore focuses on isolating and characterizing MRS strains detected in milk used in the production of...
Tue, 12/08/2020 - 22:12
Aim Fluorescent semiconductor nanoparticles or Quantum Dots (QDs) have excellent properties as photosensitizers in photodynamic therapy. This is mainly consequence of their nanometric size and the generation of light‐activated redox species. In previous works we have reported the low‐cost biomimetic synthesis of glutathione (GSH) capped QDs (CdTe‐GSH QDs) with high biocompatibility. However, no...
Tue, 12/08/2020 - 22:12
Aims To better understand the persistence dynamics of enteropathogenic bacteria in freshwater wetland habitats, we constructed lab‐scale mesocosms planted with two different wetland plant species using a sub‐surface flow wetland design. Mesocosms were treated with either a high‐ quality or a poor‐quality water source to examine the effects of water quality exposure and plant species on...
Tue, 12/08/2020 - 18:03
Background: Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected millions of individuals since December 2019, resulting in significant morbidity and mortality globally. During the 1918 Influenza Pandemic, it was observed that influenza was associated with bacterial co-infections. However, empirical or prophylactic antibiotic...
Tue, 12/08/2020 - 16:00
Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In...
Tue, 12/08/2020 - 16:00
Campylobacter jejuni express the high temperature requirement protein A (HtrA), a secreted serine protease, which is implicated in virulence properties of the pathogen. Previous studies have shown that C. jejuni HtrA can cleave the epithelial transmembrane proteins occludin and E-cadherin in the tight and adherens junctions, respectively. In the present report, we studied the interaction of HtrA...
Tue, 12/08/2020 - 10:02
The Yersinia genus comprises pathogens that are able to adapt to an environmental life cycle stage as well as to mammals. Yersinia enterocolitica strain W22703 exhibits both insecticidal and nematocidal activity conferred by the tripartite toxin complex (Tc) that is encoded on the 19 kb pathogenicity island Tc-PAIYe. All tc genes follow a strict temperature regulation in that they are silenced at...
Sun, 12/06/2020 - 06:12
Author(s): Shaimaa F. Mouftah, José F. Cobo-Díaz, Avelino Álvarez-Ordóñez, Ahmed Mousa, Jessica K. Calland, Ben Pascoe, Samuel K. Sheppard, Mohamed Elhadidy One of the emerging conundrums of Campylobacter food-borne illness is the bacterial ability to survive stressful environmental conditions. We evaluated the heterogeneity among 90 C. jejuni and 21 C. coli isolates from different sources in...
Sat, 12/05/2020 - 06:27
Author(s): Jabila Mary Thankaraj Rajam, Rajaretinam Rajesh Kannan, Muthamil Iniyan Appadurai, Carlton Ranjith Wilson Alphonse, Nandhagopal Soundarapandian, Vinita Vishwakarma, Prakash Vincent Samuel Gnana β-lactamase inhibitors are potent synergistic drugs to deteriorate the multidrug-resistant bacteria. Here, we report the β-lactamase inhibitory ability of kalafungin isolated from a marine...
Sat, 12/05/2020 - 06:26
Author(s): Rubén Agregán, Paulo E.S. Munekata, Wangang Zhang, Jian Zhang, Cristina Perez-Santaescolástica, José M. Lorenzo Salmonella is a pathogen that can contaminate a wide variety of foods and caused many outbreaks worldwide. Nowadays, the food industry mainly uses thermal processing to improve the safety and shelf life of foods. However, thermal treatment such as pasteurization may affect...
Sat, 12/05/2020 - 06:18
Author(s): Kosar Gharib-Naseri, Sarbast Kheravii, Chake Keerqin, Robert A. Swick, Mingan Choct, Shu-Biao Wu The primary cause of necrotic enteritis (NE) disease in chickens is the NetB-positive Clostridium perfringens bacterium. Many factors are known to affect the severity of NE in the challenge models of broiler chickens, and one of these factors is the virulence of C. perfringens strain. This...
Sat, 12/05/2020 - 06:18
LWT
Author(s): Junyi Zhang, Jingyi Wang, Dandan Zhao, Jianxiong Hao The disinfection efficacy of slightly acidic electrolyzed water (SAEW) has been recognized in food industry. However, the application of single SAEW limited its disinfection potential. The efficacy of the two-step disinfection with SAEW for the reduction of L. monocytogenes contamination on different food raw materials was evaluated...
Sat, 12/05/2020 - 00:00
Botulinum toxin type A (BoNT-A) is an effective treatment for post-stroke spasticity; however, some patients cannot access treatment until ≥1 year post-stroke. This Brazilian post-marketing study (NCT02390206) assessed the achievement of person-centered goals in patients with chronic post-stroke spasticity after a BoNT-A injection. Patients had a last documented stroke ≥1 year...
Sat, 12/05/2020 - 00:00
Bacterial foodborne infections, including meat-derived infections, are globally associated with diseases and some deaths. Antibiotics are sometimes used to treat bacterial infections. The use of antibiotics by farmers contributes to the development of resistance by foodborne pathogens. This study aimed to investigate the antibiotics used by farmers and the occurrence of antibiotic-resistant...
Fri, 12/04/2020 - 22:14
Shiga toxin‐producing Escherichia coli (STEC) are foodborne pathogens responsible for global outbreaks. This study was conducted to investigate the occurrence of ‘gang of five’ STEC serogroups (O26, O103, O111, O145, O157) on Belgian dairy cattle farms by overshoe (OVS) sampling, and to evaluate the presence of virulence genes in the obtained isolates. A total of 88 OVS, collected from the pen...
Fri, 12/04/2020 - 16:00
Quantitative PCR analysis shows that the virulence plasmid of Salmonella enterica serovar Typhimurium (pSLT) is a low-copy-number plasmid, with 1–2 copies per chromosome. However, fluorescence microscopy observation of pSLT labeled with a lacO fluorescent tag reveals cell-to-cell differences in the number of foci, which ranges from 1 to 8. As each focus must correspond to ≥1 plasmid copy, the...
Fri, 12/04/2020 - 16:00
Probiotics are non-pathogenic microorganisms that can interact with the gastrointestinal microbiota. They have numerous beneficial health effects that include enhancement of the host immune response, antiallergic, antimicrobial, anti-cancer, and anti-inflammatory properties. Probiotics are capable of restoring the impaired microbiome of a dysbiotic gut. They can be isolated from different...
Fri, 12/04/2020 - 16:00
Macrophages are the first encounters of invading bacteria and are responsible for engulfing and digesting pathogens through phagocytosis leading to initiation of the innate inflammatory response. Intracellular digestion occurs through a close relationship between phagocytic/endocytic and lysosomal pathways, in which proteolytic enzymes, such as cathepsins, are involved. The presence of cathepsins...
Fri, 12/04/2020 - 16:00
Pasteurella multocida is responsible for a highly infectious and contagious disease in birds, leading to heavy economic losses in the chicken industry. However, the pathogenesis of this disease is poorly understo...
Fri, 12/04/2020 - 04:02
Staphylococcus aureus is a common cause of invasive and life-threatening infections that are often multidrug resistant. To develop novel treatment approaches, a detailed understanding of the complex host–pathogen interactions during infection is essential. This is particularly true for the molecular processes that govern the formation of tissue abscesses, as these heterogeneous structures are...
Fri, 12/04/2020 - 04:02
Fresh and fresh-cut tomatoes are high in phytonutrients. But the illness outbreaks associated with contaminated tomatoes have significantly impacted public health and the industry’s economic well-being. Scientific information is critically needed to develop an effective, practical food safety standard to reduce pathogen contamination.  The aim of this study was to assess factors impacting the...
Fri, 12/04/2020 - 04:02
Outbreaks and recalls associated with microbial contamination of powdered foods have raised concern for the safety of the spray drying process and its products. However, little research on the fate of bacteria during the spray drying process has been done, leaving much unknown about the risks of contamination in spray dryers. Therefore, quantifying the contamination levels of  Salmonella  and ...
Fri, 12/04/2020 - 04:02
Beef slaughter establishments employ many different interventions to help minimize occurrence of pathogens in their products. This study explored the effectiveness of various common interventions on microbial load using the results of the Beef-Veal Carcass Baseline Survey conducted in 2014–2015. For that baseline, FSIS analyzed swab samples taken from 1,135 carcasses at 139 establishments. These...
Fri, 12/04/2020 - 04:02
Consumption of animal-derived meat products is suspected as an important exposure route to antimicrobial resistance (AMR), as the presence of antimicrobial-resistant bacteria (ARB) along the beef supply chain is well-documented. A retail-to-fork quantitative exposure assessment was established to compare consumers’ exposure to various ARB due to the consumption of ground beef with and without “...
Thu, 12/03/2020 - 22:17
Antimicrobial resistance is a worldwide public health threat; hence, current trends tend to reduce antimicrobial use in food‐producing animals and to monitor resistance in primary production. This study aimed at evaluating the impact of antimicrobial use and production system on swine farms in the antimicrobial resistance of Campylobacter, Salmonella and Staphylococcus, the main zoonotic...
Thu, 12/03/2020 - 22:17
The environmental reservoirs of different serogroups of V.cholerae causing cholera in the flowing freshwater bodies of the tribal areas of Odisha are not known. So the present study was conducted from June‐2017 to March‐2020 to find out the environmental reservoirs of V.cholerae serogroups in the water and plankton samples collected from the river, nala, stream and chua from Rayagada district....
Thu, 12/03/2020 - 22:16
The application of bacteriophage P100 to reduce incidence of Listeria sp. in ready‐to‐eat (RTE) food production environments was investigated. Data indicate the application of Listeria specific phage P100 in RTE food production environments can reduce incidence and be considered an additional intervention strategy for controlling this pathogen on NFCS. Abstract Listeria monocytogenes is a...
Thu, 12/03/2020 - 22:16
This study evaluated the effect of lactic acid stress on E. coli O26 biofilm formation at different temperatures. The strain 126Z with strong capacity and strain G13Z2 with medium capacity were selected for biofilm formation experiment in acidic TSB (pH 4.0 and 5.0, adjusted with lactic acid) at 15°C and 25°C, respectively. The effect of temperature on biofilm formation of different capacity...
Thu, 12/03/2020 - 22:12
Aims The aims of this study were to investigate the effects of probiotics and antibiotics on microbial composition, short chain fatty acids (SCFAs) concentration and free fatty acid receptor 2/3 (FFAR2/3) expression in boiler chickens. Methods and Results 150 one‐day‐old male broilers were randomly allocated into 3 groups, control (CON) group, probiotics (PB) group and antibiotics (ATB) group....
Thu, 12/03/2020 - 22:11
ABSTRACT Background Fruit and vegetable consumption has increased among masses due to their tremendous health benefits. However, recent studies have shown that contaminated products may serve as a vehicle for foodborne pathogens and harmful chemicals. Therefore, fresh vegetables must be decontaminated before consumption to ensure food safety. Results In this study, the combined decontamination...
Thu, 12/03/2020 - 22:11
BACKGROUND Carbon dots (C‐dots) with antimicrobial activity were synthesized from the white mulberry extract in order to fabricate anti‐listeria nanopaper using bacterial nanocellulose (BNC). A highly dispersed synthesized C‐dots with a size smaller than 10 nm (~4.9 nm) was impregnated into BNC by an ex‐situ coating method and mechanical, morphological, UV‐protectant, and antibacterial activity...
Thu, 12/03/2020 - 16:00
It is now well known that the quorum sensing (QS) mechanism coordinates the production of several virulence factors and biofilm formation in most pathogenic microorganisms. Aeromonas hydrophila is a prime pathogen responsible for frequent outbreaks in aquaculture settings. Recent studies have also continuously reported that A. hydrophila regulates virulence factor production and biofilm formation...
Thu, 12/03/2020 - 16:00
External signals are crucial for bacteria to sense their immediate environment and fine-tune gene expression accordingly. The foodborne pathogen Listeria monocytogenes senses a range of environmental cues in order to activate or deactivate the virulence-inducing transcriptional factor PrfA during transition between infectious and saprophytic lifecycles. Chitin is an abundant biopolymer formed...
Thu, 12/03/2020 - 16:00
The rise of antibiotic resistance is not only a challenge for human and animal health treatments, but is also posing the risk of spreading among bacterial populations in foodstuffs. Farmed fish-related foodstuffs, the food of animal origin most consumed worldwide, are suspected to be a reservoir of antibiotic resistance genes and resistant bacterial hazards. However, scant research has been...
Thu, 12/03/2020 - 10:02
The complete genome of Salmonella enterica subsp. enterica serovar Kottbus strain Kharkiv (serogroup C2-C3), which was isolated from a commercial pork production facility in Kharkiv, Ukraine, was assembled using long-read Nanopore sequences. A single circular contig (4,799,045 bp) comprised a complete chromosome encoding antibiotic resistance, highlighting the risk of cross-species livestock and...
Thu, 12/03/2020 - 10:02
Pneumonic pasteurellosis, caused by Pasteurella multocida, is a common respiratory infection of ruminants that has major economic and welfare implications throughout the world. Here, we report the annotated genome sequences of seven pathogenic strains of P. multocida that were isolated from cattle in the United Kingdom.
Thu, 12/03/2020 - 10:02
We present the complete genomes of four Brucella suis biovar 2 isolates that were obtained from wild boars in Switzerland in 2008 and 2009. Genomes were sequenced with PacBio technology, contained two chromosomes each, had a genome size of 3.3 Mbp, and contained more than 3,225 genes per genome.
Thu, 12/03/2020 - 10:02
The complete genome sequence of a Shiga toxin-producing Escherichia coli (STEC) O26:H11 strain, MBT-5 (sequence type 21 [ST21], stx1a, stx2a, eae, ehxA), and two draft genome sequences of Listeria monocytogenes strains MBT-6 and MBT-7 belonging to the virulent sequence types 1 (ST1, clonal complex 1 [CC1]) and 59 (ST59, CC59), respectively, were determined. The strains were isolated in 2015 from...
Thu, 12/03/2020 - 10:02
Serratia marcescens strain ZZCCN01 was isolated from the cardiac blood of a dead beef cow with a lung infection and a foam-like secretion from the nostril. Here, we introduce the 5.1-Mb draft genome sequence, which comprises 105 scaffolds, and the corresponding annotation.
Thu, 12/03/2020 - 06:25
Author(s): Hyeon Woo Park, Guoying Chen, Cheng-An Hwang, Lihan Huang The aim of this study was to investigate the effect of water activity (aw) on inactivation of Listeria monocytogenes using gaseous chlorine dioxide (ClO2 (g)) under room temperature. Surface-inoculated tryptic soy agar (TSA) plates adjusted to 9 different water activity levels ranging from 0.994 to 0.429 were used as samples...
Thu, 12/03/2020 - 06:21
Author(s): Ryan Cuzzi, Vivian Ly, Valeria R. Parreira, Alma Fernanda Sanchez-Maldonado, Jeffrey M. Farber The survival of Listeria monocytogenes was assessed during long-term storage on three dried fruits: dried apples, raisins and dried strawberries. Using sand as a carrier, the dried fruits were dry-inoculated with a four-strain cocktail of L. monocytogenes to achieve numbers of 4.0 to 4.6 log...
Thu, 12/03/2020 - 00:00
Enterotoxigenic Escherichia coli (ETEC) produces the heat-stable enterotoxin b (STb), which is responsible for secretory diarrhea in humans and animals. This toxin is secreted within the intestinal lumen of animals and humans following ETEC colonization, becoming active on enterocytes and altering fluid homeostasis. Several studies have outlined the nature of this toxin and its effects on gut...
Wed, 12/02/2020 - 22:11
Journal of Applied Microbiology, Accepted Article. This study evaluated the effects of Bacillus amyloliquefaciens (B. amyloliquefaciens) TL106 isolated from Tibetan pigs’ feces, on the growth performance, immune response, intestinal barrier function, morphology of jejunum, cecum and colon, and gut microbiota in the mice with enterohemorrhagic Escherichia coli (EHEC)‐induced intestinal diseases.
Wed, 12/02/2020 - 16:00
Acid treatment is commonly used for controlling or killing pathogenic microorganisms on medical devices and environments; however, inadequate acid treatment may cause acid tolerance response (ATR) and offer cr...
Wed, 12/02/2020 - 06:12
Author(s): Débora Cristina Sampaio de Assis, Thaís Michelle Liziere da Silva, Rommel Furst Brito, Lorraine Cássia Gonçalves da Silva, William Gustavo Lima, Júlio César Moreira Brito We conducted a systematic review and meta-analysis to determine the rate of contamination in bovine meat and meat products with Shiga toxin-producing Escherichia coli (STEC) in Brazil over the last fifteen years. Data...
Wed, 12/02/2020 - 04:02
Bacteria utilize versatile strategies to propagate infections within human cells, e.g., by the injection of effector proteins, which alter crucial signaling pathways. One class of such virulence-associated proteins is involved in the AMPylation of eukaryotic Rho GTPases with devastating effects on viability. In order to get an inventory of AMPylated proteins, several technologies have been...
Tue, 12/01/2020 - 12:01
Background Typhoid fever is caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and can lead to systemic illness and complications. We aimed to characterize typhoid-related ileal perforation in the context of the population-based Surveillance of Enteric Fever in Asia Project (SEAP) in Bangladesh, Nepal and Pakistan. Methods Between September 2016 and September 2019, all...
Tue, 12/01/2020 - 12:01
Background Clinicians have limited therapeutic options for enteric as a result of increasing antimicrobial resistance, and therefore typhoid vaccination is recommended as a preventive measure. As a part of the Surveillance for Enteric Fever in Asia Project (SEAP), we investigated the extent measured the burden of antimicrobial resistance (AMR) among confirmed enteric fever cases in Bangladesh,...
Tue, 12/01/2020 - 12:01
Background Enteric fever, a bacterial infection caused by Salmonella enterica serotypes Typhi and Paratyphi A, frequently presents as a nonlocalizing febrile illness that is difficult to distinguish from other infectious causes of fever. Blood culture is not widely available in endemic settings and, even when available, results can take up to 5 days. We evaluated the diagnostic performance of...
Tue, 12/01/2020 - 10:02
FliA is a broadly conserved factor that directs transcription of genes involved in flagellar motility. We previously identified FliA-transcribed genes in Escherichia coli and Salmonella enterica serovar Typhimurium, and we showed that E. coli FliA transcribes many unstable, non-coding RNAs from intragenic promoters. Here, we show that FliA in S. Typhimurium also directs transcription of large...

Pages