Antibiotic residues

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Wed, 12/04/2019 - 03:27
Lipopeptides are important non-ribosomal peptide synthetases (NRPSs) products with broad therapeutic potential in biotechnology and biopharmaceutical applications. Fatty acyl modifications in N-terminal of lipopeptides have attracted wide interest in the engineering processes of altered fatty acyl selectivity. In this study, we focused on the starter condensation domain of antibiotic A54145 (...
Sun, 11/24/2019 - 03:48
Author(s): Antoine Rostang, Jacques Devos, Christophe ChartierAbstractEprinomectin (EPM) has been recently granted a marketing authorisation in the European Union for use in goats, with a zero-day milk withdrawal period. Considering the high prevalence of benzimidazole resistance worldwide and the economic implications of managing milk residues, EPM may today be considered the main (or even the...
Fri, 11/22/2019 - 03:37
Multidrug efflux pumps play an important role in antibiotic resistance in bacteria. In Pseudomonas aeruginosa, the MexXY pump provides intrinsic resistance to many antimicrobials, including aminoglycosides. The expression of the mexXY operon is negatively regulated by the MexZ repressor. This repression is alleviated in response to antibiotic-induced ribosome stress, which results in increased...
Fri, 11/22/2019 - 03:26
ABSTRACT The poultry industry has recently undergone transitions into antibiotic free production, and viable antibiotic alternatives, such as probiotics, are necessary. Through in ovo probiotic inoculation, beneficial microflora development in the gastrointestinal tract may occur prior to hatch without negatively impacting chick performance. Therefore, the objective of the present study was to...
Thu, 11/21/2019 - 03:25
It has been reported that Heteropodatoxin3 (HpTx3), a peptidic neurotoxin purified from the venom of the spider species Heteropoda venatoria, could inhibit Kv4.2 channels. Our present study newly found that HpTx3 also has potent and selective inhibitory action on Nav1.7, with an IC50 of 135.61 &plusmn; 12.98 nM. Without effect on the current&ndash;voltage (I-V) relationship of Nav1.7,...
Tue, 11/19/2019 - 03:34
Author(s): Bian-hua Zhou, Liu-shu Jia, Hong-wei Guo, Hai-yan Ding, Jing-yun Yang, Hong-wei WangAbstractEimeria tenella, an obligate intracellular parasite, can actively invade the cecal epithelial cells of chickens and cause severe enteric disease. Eukaryotic elongation factor 2 (eEF2) plays a major role in protein synthesis and cell survival. This study aims to explore the exact mechanisms...
Sat, 11/16/2019 - 03:37
by Yutaka Takaoka, Atsuko Takeuchi, Aki Sugano, Kenji Miura, Mika Ohta, Takashi Suzuki, Daisuke Kobayashi, Takuji Kimura, Juichi Sato, Nobutaro Ban, Hisahide Nishio, Toshiyuki Sakaeda UDP-glucuronosyltransferase 1A1 (UGT1A1) is an enzyme that is found in the endoplasmic reticulum membrane and can reportedly have a large number of amino acid substitutions that result in the reduction of...
Sat, 11/16/2019 - 03:25
A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at...
Wed, 11/13/2019 - 03:37
by Marlene Belmar, Sharif Aly, Betsy M. Karle, Richard V. Pereira The objective of this study was to identify factors affecting the accuracy of four commercial tests for ceftiofur drug residue in milk samples from bulk tank waste milk (WM). WM samples were collected from 12 California dairy farms which were initially tested using liquid chromatography (LC-MS/MS) to confirm their negative status...
Sun, 11/10/2019 - 03:25
Flumequine (FLU) is widely used as an antibiotic in the treatment and control of animal diseases, which may result in the presence of residues harmful to humans in food products of animal origin. A novel and fast biomimetic enzyme-linked immunosorbent assay (BELISA) was developed for the determination of FLU based on a molecularly imprinted polymer (MIP) as a selective affinity agent. With an...
Sat, 11/09/2019 - 03:35
Author(s): P. Quintanilla, M. Cornacchini, M.I. Hernando, M.P. Molina, I. EscricheAbstractThis study evaluated instrumental parameters (chemical composition, colour, texture and microstructure) and sensory characteristics of goat cheese (60-day maturation) made with milk spiked with oxytetracycline at different levels: 0, 50, 100 (maximum residue limit; MRL) and 200 μg kg-1. Additionally, the...
Wed, 11/06/2019 - 03:26
Galactosaminogalactan (GAG) is an insoluble aminosugar polymer produced by Aspergillus fumigatus and has anti-inflammatory properties. Here, the minimum glycosidic sequences required for the induction of IL-1Ra by peripheral blood mononuclear cells (PBMCs) was investigated. Using chemical degradation of native GAG to isolate soluble oligomers, we have found that the de-N-acetylation of...
Tue, 11/05/2019 - 03:28
ABSTRACT BACKGROUND The low cost of aminoglycoside (AMG) antibiotics facilitates their excessive use in animal husbandry and agriculture sector. This scenario has led to the occurrence of residues in the food chain. After several years of AMGs use in antibacterial therapy, resistance to streptomycin has begun to appear. The method lacks AMG specificity previously reported against a single...
Tue, 11/05/2019 - 03:28
The present study was envisaged with an aim to determine the occurrence of antibiotic residues; enrofloxacin, oxytetracycline, penicillin G, sulphamethoxazole and chloramphenicol as well as mycotoxins; aflatoxin M1 and ochratoxin A in raw milk samples collected from individual animals from dairy farms located in 9 districts of Punjab, India. A total of 168 raw milk samples were collected and...
Sun, 11/03/2019 - 02:35
Author(s): Johanne Ellis-Iversen, Anne Mette Seyfarth, Helle Korsgaard, Valeria Bortolaia, Nanna Munck, Anders DalsgaardAbstractAntimicrobial resistance (AMR) genes, resistant bacteria and antimicrobial residues may be transferred to humans through consumption of fish and prawns raised in aquaculture. This study investigated AMR in E. coli and enterococci introduced to Denmark via prawns and...
Wed, 10/30/2019 - 03:38
ABSTRACT E165R, a highly specific dUTP nucleotidohydrolase (dUTPase) encoded by the African swine fever virus (ASFV) genome, is required for productive replication of ASFV in swine macrophages. Here, we solved the high-resolution crystal structures of E165R in its apo state and in complex with its product dUMP. Structural analysis explicitly defined the architecture of the active site of the...
Wed, 10/30/2019 - 03:26
Potential synergism between florfenicol (FF) and thiamphenicol (TAP) was investigated for in vitro efficacy against Actinobacillus pleuropneumoniae and/or Pasteurella multocida as well as in vivo efficacy in swine. Among isolates of A. pleuropneumoniae (n = 58) and P. multocida (n = 79) from pigs in Taiwan that were tested, high percentages showed resistance to FF (52 and 53%, respectively) and...
Fri, 10/25/2019 - 03:45
Author(s): Mengting Wang, Bin Peng, Yang Wang, Na Zhao, Zhili Xiong, Longshan ZhaoAbstractA novel multi-residue method was developed for the simultaneous analysis of tetracyclines and β-receptor agonists in chicken by coupling pressurized liquid extraction (PLE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The parameters of PLE method was optimised,...
Fri, 10/25/2019 - 03:44
Author(s): Xue Hou, Xu Xu, Xiaoying Xu, Mei Han, Shiting QiuAbstractA multi-class screening method for the determination of veterinary drugs and pesticides in eggs was developed by high performance liquid chromatography quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS). A single-injection workflow was developed for qualitative analysis, and method validation was conducted in terms of...
Tue, 10/22/2019 - 03:33
A previously unreported xylated glycosaminoglycan with a β‐D‐Xylop(1 → 3)‐(⋯ → 4)‐GlcpA(1 → 3)‐GlcpNAc(1 → ⋯) framework was isolated to homogeneity from marine buccinid gastropod Babylonia spirata, and was found to possess potential antioxidant and 5‐lipoxygenase attenuation activities. The isolated metabolite might be anticipated as potential naturally‐derived bioactive constituent in functional...
Sun, 10/20/2019 - 03:41
Author(s): Gaurav Kumar, Bagre Issa, Sarmistha Biswal, Diamond Jain, Amitabha Bhattacharjee, Anindya S. GhoshAbstractNew Delhi Metallo beta-lactamase (NDM) is of significant public health concern due to its enormous potential to hydrolyse all the major beta-lactams including carbapenems. Previous reports indicate that the amino acid substitutions affect NDM activity despite being located outside...
Fri, 10/18/2019 - 03:41
by Nematollah Gheibi, Mohammad Ghorbani, Hanifeh Shariatifar, Alireza Farasat Calprotectin is a heterodimeric protein complex which consists of two subunits including S100A8 and S100A9. This protein has a major role in different inflammatory disease and various types of cancers. In current study we aimed to evaluate the structural and thermodynamic changes of the subunits and the complex in...
Fri, 10/18/2019 - 03:37
Author(s): Kaihang Xu, Jun Wang, Han Gong, Yizheng Li, Lei Zhou, Muting YanAbstractThe concentration of 8 antibiotics and 21 antibiotic resistance genes were investigated in the coastal areas of Guangdong, China. Total concentrations of antibiotics ranged from 0.43 ng/L to 1040.31 ng/L. The concentrations of tetracyclines were much higher than that of sulfonamides in most sampling sites. The...
Thu, 10/17/2019 - 03:28
Antimicrobial residues in milk have been discussed as a possible selector for Enterobacteriaceae that produce extended-spectrum β-lactamases (ESBL) in dairy herds. Such residues are found in waste milk after antibiotic treatment of mastitis, but antibiotic dry cow therapy might also lead to antibiotic residues in colostrum and in milk during early lactation. While it is known that feeding of...
Wed, 10/16/2019 - 03:41
ABSTRACT To further our understanding of one-carbon metabolism in the protozoan parasite Leishmania, we conducted genomic screens to study how the parasite responded to sinefungin (SNF) selection. SNF is a structural analogue of S-adenosylmethionine (AdoMet), a key methyl group donor to a number of biomolecules. One screen consisted of sequencing SNF-resistant mutants generated by stepwise...
Fri, 10/11/2019 - 03:29
Antimicrobial peptides (AMPs) represent the largest group of endogenous compounds and serves as a novel alternative to traditional antibiotics for the treatment of pathogenic microorganisms. Moricin is an important α-helical AMP plays a crucial role in insect humoral defense reactions. The present study was designed to identify and characterize novel AMP moricin (Px-Mor) from diamondback moth (...
Wed, 10/09/2019 - 03:29
Abstract Presence of bacterial contaminants at levels > 107 colony forming units per milliliter (CFU/mL) during ethanol production processes reduces the alcoholic fermentation yield by 30%. Antibiotics are currently used to control contamination, but their residues may be detected in yeast extract, restricting this by-product trade to several countries. Thus, the objective of this study was...
Mon, 09/30/2019 - 17:10
Carbapenem-resistant Enterobacteriaceae are a significant threat to public health, and a major resistance determinant that promotes this phenotype is the production of the OXA-48 carbapenemase. The activity of OXA-48 towards carbapenems is a puzzling phenotype as its hydrolytic activity against doripenem is non-detectable. To probe the mechanistic basis for this observation, we determined the 1.5...
Mon, 09/30/2019 - 16:54
ABSTRACT Mannitol-1-phosphate dehydrogenase (M1PDH) is a key enzyme in Staphylococcus aureus mannitol metabolism, but its roles in pathophysiological settings have not been established. We performed comprehensive structure-function analysis of M1PDH from S. aureus USA300, a strain of community-associated methicillin-resistant S. aureus, to evaluate its roles in cell viability and virulence under...
Mon, 09/30/2019 - 16:54
ABSTRACT The filoviruses are etiological agents of life-threatening hemorrhagic fever with high mortality rate and risk of potential outbreak. Among members of this family, the Ebola (EBOV), Sudan (SUDV), and Marburg (MARV) viruses are considered the most pathogenic for humans. The ebolavirus nucleoprotein (NP) is the most abundant protein in infected cells and is essential for viral...
Sat, 09/28/2019 - 03:28
Author(s): Barbara Spolaore, Julián Fernández, Bruno Lomonte, Maria Lina Massimino, Fiorella TonelloAbstractAlmost all animal venoms contain secretory phospholipases A2 (PLA2s), 14 kDa disulfide-rich enzymes that hydrolyze membrane phospholipids at the sn-2 position, releasing lysophospholipids and fatty acids. These proteins, depending on their sequence, show a wide variety of biochemical, toxic...
Tue, 09/24/2019 - 03:40
Carbapenem-hydrolyzing class D carbapenemases (CHDLs) are enzymes that produce resistance to the last-resort carbapenem antibiotics, severely compromising the available therapeutic options for the treatment of life-threatening infections. A broad variety of CHDLs, including OXA-23, OXA-24/40, and OXA-58, circulate in Acinetobacter baumannii, while the OXA-48 CHDL is predominant in...
Tue, 09/24/2019 - 03:40
Glutamate amidation, a secondary modification of the peptidoglycan, was first identified in Staphylococcus aureus. It is catalyzed by the protein products of the murT and gatD genes, which are conserved and colocalized in the genomes of most sequenced Gram-positive bacterial species. The MurT-GatD complex is required for cell viability, full resistance to β-lactam antibiotics, and resistance...
Tue, 09/24/2019 - 03:40
Oral candidiasis (OC) caused by the fungal pathogen Candida albicans is the most common opportunistic infection in immunocompromised populations. The dramatic increase in resistance to common antifungal agents has emphasized the importance of identifying alternative therapeutic options. Antimicrobial peptides have emerged as promising drug candidates due to their antimicrobial properties;...
Mon, 09/23/2019 - 03:29
Author(s): Fabián Lorenzo-Diaz, Inmaculada Moreno-Córdoba, Manuel EspinosaAbstractStreptococcus pneumoniae is a pathogenic and opportunistic Gram-positive bacterium that is the leading cause of community-acquired respiratory diseases, varying from mild- to deathly- infections. The appearance of antibiotic-resistant isolates has prompted the search for novel strategies and targets to tackle the...
Sun, 09/22/2019 - 03:25
Ellagic acid reversibly and effectively inhibited tyrosinase in a mixed manner. Ellagic acid inhibited cell proliferation and induced apoptosis in B16 cells. Hydrogen bond and electrostatic force existed between ellagic acid and tyrosinase. Abstract The activities of ellagic acid in inhibiting mushroom tyrosinase and cell proliferation were evaluated in this research. The results of enzyme...
Sun, 09/22/2019 - 03:25
The oligosaccharide Hep‐2 from cultured mycelium of Hericium erinaceus was isolated by the method of water extraction, alcohol precipitation, and column chromatography. Hep‐2 consists of galactose, and glucose with 1,080 Da molecular weight and it is an oligosaccharide mixture of 2–7 sugars by LC‐MSn and includes three types of sugar residues with no branches by methylation methods. Hep‐2 possess...
Sun, 09/22/2019 - 03:23
The eukaryotic initiation factor 4E (eIF4E) specifically recognizes the 5′ mRNA cap, a rate-limiting step in the translation initiation process. Although the 7-methylguanosine cap (MMGcap) is the most common 5′ cap structure in eukaryotes, the trans-splicing process that occurs in several organism groups, including nematodes and flatworms, leads to the addition of a trimethylguanosine cap (TMGcap...
Sun, 09/22/2019 - 03:22
There is a significant increase in the discovery of new antimicrobial compounds in recent past to combat drug resistant pathogens. Members of the genus Bacillus and related genera have been screened extensively due to their ability to produce wide range of antimicrobial compounds. In this study, we have isolated and characterized a new antimicrobial peptide from a marine bacterium identified as...
Sat, 09/21/2019 - 03:36
Bacteria have acquired multiple mechanisms to evade the lethal effects of current therapeutics, hindering treatment of bacterial infections, such as those caused by the pathogen Pseudomonas aeruginosa, which is responsible for nosocomial and cystic fibrosis lung infections. One resistance mechanism involves membrane-embedded multidrug efflux pumps that can effectively extrude an array of...
Sat, 09/21/2019 - 03:35
Author(s): Sandra Unsleber, Wolfgang Wohlleben, Evi StegmannAbstractPeptidoglycan (PG) is a bacteria specific cell surface layer that ensures the bacterial shape and integrity. The two actinomycetes Amycolatopsis balhimycina and Microbispora sp. PTA-5024 are producers of PG targeting antibiotics. To prevent the binding of their secreted product to their own PG, they developed specific self-...
Sat, 09/21/2019 - 03:32
Author(s): Gamal A Elmowalid, Marwa I Abd El-Hamid, Ashraf M Abd El-Wahab, Mohammad Atta, Gamal Abd El-Naser, Adel M AttiaAbstractAdministration of antibiotics as feed additives in broilers resulted in prompting of some undesirable effects such as the rising emergence of multi-drug resistant (MDR) bacteria, so scrutinizing for new alternatives like herbs is the up to date task for global health....
Sat, 09/21/2019 - 03:31
Author(s): Mahesh Agarwal, Tanmay Mondal, Biplab BoseAbstractMembrane-anchored heparin-binding EGF-like growth factor (HB-EGF) is the receptor for diphtheria toxin (DT). Mutated or truncated, non-toxic DT has been used earlier for HB-EGF-targeted drug delivery and to modulate HB-EGF signaling. In the present work, we have synthesized a peptide corresponding to a 26 amino acid long stretch of the...
Mon, 09/16/2019 - 11:34
Abstract In the present study, we analyzed the tilmicosin residues in eggs as well as the dietary risks posed by the residual drug to Chinese consumers. Tilmicosin was administrated to laying hens via drinking water in doses of 100 mg/L and 300 mg/L (dose 1 and dose 2) for 5 days. Its residues distribution within egg matrices were detected using a QuEChERS method coupled with liquid...
Wed, 09/11/2019 - 00:00
In this study, we developed a microbiological inhibition method for the rapid screening of antibiotics in milk with Geobacillus stearothermophilus ATCC12980 as an indicator bacterium and an easy sample pretreatment. We observed that the limits of detection of the kit for 34 common antibiotic residues in milk, including β-lactams (13), aminoglycosides (6), tetracyclines (4), sulfonamides (6),...
Sat, 09/07/2019 - 01:08
Publication date: Available online 5 September 2019 Environmental ResearchAuthor(s): Abdelmonaim Azzouz, Laura Palacios Colón, Badredine Souhail, Evaristo BallesterosAbstractAn integrated study was conducted to determine the presence of six types of endocrine disrupting chemicals (bisphenol A, triclosan, two alkylphenols, two phenylphenols, eleven organophosphorus pesticides and seven parabens)...
Wed, 08/21/2019 - 01:05
Available online 20 August 2019 Author(s): DanNa Zhou, YaChao Li, LingLi Huang, MingRong Qian, Dong Li, GuiZhi Sun, Bo YangAbstractIt is still a challenge to solve the matrix interferences in veterinary drug residue analysis. In this study, we reported a thin layer chromatography (TLC)-high-performance liquid chromatography (HPLC) method for determining total florfenicol (FF) residues, expressed...
Sat, 07/27/2019 - 01:03
Publication date: 1 December 2019 , Volume 300Author(s): Bolin Zhu, Shuang Li, Li Zhou, Qing Li, Xingjie GuoAbstractIn stock farming, illegal use of antipsychotics has caused the food safety problem. This paper presents for the first time, a multi-residues method for the simultaneous enantioselective determination of seven antipsychotics in pork, beef and lamb muscles. The behaviors of Chiralpak...

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