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Food Analytical Methods

Thu, 03/01/2018 - 00:00
An accurate, rapid, and reliable multiresidue QuEChERS method based on gas chromatography coupled with tandem mass spectrometry was developed for the determination of 235 pesticides in challenging, dry, complex herb matrices (Centaurea cyanus L., Matricaria chamomilla L., Thymus vulgaris L.). Sample mass and the type of cleanup sorbent used to estimate the procedure’s...
Thu, 03/01/2018 - 00:00
A method of hollow fiber–stir bar sorptive extraction (HF–SBSE) coupled with gas chromatography–mass spectrometry was proposed to explore the migration of eight organochlorine pesticide residues in environmental and biological matrices. HF–SBSE was a configuration for solid–phase microextraction purposes, combining the features of SBSE and hollow fiber membrane extraction...
Thu, 03/01/2018 - 00:00
Benzo[a]pyrene (BaP), a kind of polycyclic aromatic hydrocarbon, has great potential hazards to the ecosystem and human health. In this study, based on the preparation of BaP immunogen and coating antigen, a specific polyclonal antibody targeting BaP was generated, and a highly sensitive real-time immuno-PCR assay using the biotin-streptavidin system (BA-IPCR) was developed...
Thu, 03/01/2018 - 00:00
A quantum-dot-based immunochromatographic assay (QICA) and a fluorescence quenching immunochromatographic assay (FQICA) were developed to visually detect bisphenol A (BPA). The proposed QICA and FQICA were based on traditional immune competition and on fluorescence resonance energy transfer (FRET) from fluorescence donors (quantum dots) to fluorescence acceptors (colloidal...
Thu, 03/01/2018 - 00:00
A fast and simple sample preparation method based on dispersive liquid–liquid microextraction (DLLME) and ultrasonic-assisted extraction (UAE) was proposed in order to determine aflatoxin B1 (AFB1) in eggs. In this study, AFB1 was first extracted from matrixes of egg samples with acetonitrile/water 80:20 (V/V), then its eluents were utilized as dispersant of the followed UAE-...
Thu, 03/01/2018 - 00:00
In this research, for the first attempt, we successfully determined seven polycyclic aromatic hydrocarbons (PAHs) in various coffee samples using microwave-assisted extraction and dispersive liquid-liquid microextraction (MAE-DLLME) coupled with gas chromatography-mass spectrometry (GC-MS). The effects of most important variables in microextraction step were investigated and...
Thu, 02/01/2018 - 00:00
The first aim of this study was to create, test, and apply a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method, based on acetonitrile extraction, for simultaneous determination of pesticide residues in green chili pepper (fresh and dried forms). There is a group of 86 repeatedly detected pesticides in the agricultural food commodity in Al-Qassim region, Saudi...
Thu, 02/01/2018 - 00:00
A solid-phase extraction (SPE) combined with high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method was developed to determine five phenols in grease-rich food (i.e., hotpot seasoning). Sample added with isotope internal standards bisphenol A-d 4 (BPA-d 4) and 4-n-nonylphenol-d 4 (4...
Thu, 02/01/2018 - 00:00
This study describes the development of a novel reproducible molecularly imprinted quartz crystal microbalance (QCM) sensor for the accurate and sensitive analysis of the residue of enrofloxacin (ENRO) in animal-derived foods. This proposed sensor was easily fabricated by directly immobilizing molecularly imprinted polymer (MIP) of ENRO on the surface of a QCM Au chip, which...
Thu, 02/01/2018 - 00:00
The vine produces de novo compounds with small molecules, called phytoalexins, as defense response to oxidative stress, microbial infections, and UV irradiation. Such bioactive substances are trans-resveratrol and quercetin. In this study, a rapid HPLC method was developed and validated for simultaneous quantification of both phenolic compounds and was applied for their...
Thu, 02/01/2018 - 00:00
A rapid, simple, and efficient method was described for the simultaneous determination of three common antibiotic residues including erythromycin, tetracycline, and chloramphenicol in raw milk. A molecular imprinting technique combined with solid-phase extraction (SPE) was used to pretreat the test samples and then simultaneously detected with HPLC. The whole process, only...
Thu, 02/01/2018 - 00:00
A simple sensor ordered mesoporous carbon/glassy carbon electrode (OMC/GCE) is developed in this paper for melamine (Mel) detection, which is based on conversion of Mel from non-electroactive Mel to electroactive Cu-Mel complex under presence of copper ions in 0.1 M pH 9.0 borate buffer containing 20% methanol (v/v) as supporting electrolyte at the surface of OMC modified...
Mon, 01/01/2018 - 00:00
The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental conditions. Under selected conditions (using melamine as the additive), both L-...
Tue, 12/19/2017 - 00:00
Protection of the quality products and particularly Protected Designation of Origin (PDO)/Protected Geographical Indication (PGI) foods is a strategic issue in the EU economy, in terms of protection of market competition and safety. Having reliable tools for the assessment of key parameters useful for the identification of authenticity and/or frauds is therefore of main...
Tue, 12/19/2017 - 00:00
A porous β-cyclodextrin polymer (P-CDP) was used as a new sorbent for simultaneous solid-phase extraction (SPE) and determination of bisphenol A (BPA), bisphenol F (BPF), and bisphenol AF (BPAF). The P-CDP was obtained via the cross-linking of β-cyclodextrin with tetrafluoroterephthalonitrile, and then was characterized by FT-IR spectroscopy, scanning electron microscopy,...
Sun, 12/17/2017 - 00:00
Volatiles are critical for real and perceived quality of mussels. In the present study, we determined, for the very first time, the characteristic volatiles of fresh Mediterranean mussels (Mytilus galloprovincialis) and their variation during 4 days of storage at 6.0 ± 0.5 °C. During this time, volatile organic compounds (VOCs) were monitored using SPME-GC-MS. Twenty-seven VOCs...
Sun, 12/17/2017 - 00:00
Alternariol (AOH) is one of the major mycotoxins produced by various species of Alternaria fungi. Natural occurrences of AOH have been reported in various foods, including fruits; processed fruit products such as apple juice, tomato products; wheat and other grains; oilseeds and products thereof, such as sunflower seeds, oilseed rape meal, and flax seed/linseed; and pecans. In...
Tue, 12/12/2017 - 00:00
Hydrogen sulfide (H2S) has recently been recognized as the third endogenous gaseous signaling molecule besides NO and CO. At the same time, H2S is responsible for an important proportion of faulty wines with potentially large economic losses. Thus, the H2S in wine not only contributes negatively to wine aroma but also faces a problem of food safety. So an efficient and...
Thu, 12/07/2017 - 00:00
Acrylamide (ACR) is a potential carcinogen and is found in thermally processed foods such as potato chips, biscuits, baby foods, coffee, etc. In this paper, l-cysteine-capped Mn-doped ZnS quantum dots (QDs) as phosphorescent probes were used for the determination of ACR. This method based on quenching of the phosphorescence intensity of the QDs with the interaction of ACR. Room...
Wed, 12/06/2017 - 00:00
In this study, a rapid and sensitive method of real-time loop-mediated isothermal amplification (Rti-LAMP) assays was developed for quantification and discrimination of viable and heat-killed E. coli O157:H7 cells treated with low concentration of ethidium bromide monoazide (EMA). Four micrograms per milliliter of EMA was chosen as the optimal concentration which did not...
Sun, 12/03/2017 - 00:00
Pyrrolizidine alkaloids (PAs) are secondary metabolites produced by plants as a chemical defense against herbivores. Plants containing PAs are widely distributed in almost all geographical regions posing a risk of honey contamination. To provide safety of honey and decrease the potential risk for the consumers, a sensitive method based on gas chromatography-mass spectrometry...
Fri, 12/01/2017 - 00:00
A voltammetric method for fast and high sensitive determination of Bisphenol A (BPA) using glassy carbon electrode (GC) modified with carbon black nanoparticles (CB) was developed. Cyclic voltammetry study in the 0.1 M phosphates of pH = 7.0 gave single anodic peak at 578 mV. Adsorption-controlled oxidation of BPA was found to be irreversible with the participation of two...
Fri, 12/01/2017 - 00:00
In this study, a core–shell magnetic dummy molecularly imprinted polymer (MDMIP) was prepared with a novel and simple method. The imprinting layer was synthesized directly on the surface of Fe3O4 by only one step, which avoided further modification on the exterior of magnetic core. 4,4′-Dihydroxybiphenyl (DDBP) was used as dummy template instead of bisphenol A (BPA) to...
Fri, 12/01/2017 - 00:00
Modified QuEChERS method combined with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed and validated to determine pesticide residues in dried cocoa beans. Extraction efficiency of matrix hydration effect on 19 pesticide residues was studied and optimized using 23 full factorial design with percentage recoveries as the response variable. The optimized...
Fri, 12/01/2017 - 00:00
The application of a simple, fast, and cheap sample pre-concentration technique based on the dispersive magnetic Fe3O4 nanoparticle solid-phase extraction has been developed for extraction and determination of aflatoxins B1, B2, G1, and G2 in nut and cereals. Aflatoxins were extracted from samples with a mixture of methanol–water 8:2 (v/v) and then by magnetic Fe3O4...
Fri, 12/01/2017 - 00:00
An acryloyl β-cyclodextrin (A-β-CD) monolithic column for solid-phase microextraction (SPME) and determination of carbofuran and carbaryl by high-performance liquid chromatography (HPLC) have been prepared through a “one-step” polymerization method. The synthesis conditions, including the volume of cross-linker, the ratio and volume of mixed porogenic solvent consisted of...
Fri, 12/01/2017 - 00:00
Vanilla extracts are widely used as flavoring ingredients in foods, beverages, perfumes, and pharmaceuticals. Due to the high cost of producing high-quality natural extracts, artificial flavorings, such as ethyl vanillin and coumarin, are often used. For food safety and to ensure high-quality products, it is important to differentiate between natural and artificial extracts....
Tue, 11/07/2017 - 00:00
Conventional methods to detect Salmonella in milk generally take a long time and are significantly laborious besides requiring the use of different media and reagents. Thus, this research aimed to offer an alternative methodology based on near-infrared spectroscopy (NIRS) to discriminate the presence of Salmonella in milk. For this purpose, different milk samples, contaminated...
Mon, 11/06/2017 - 00:00
A high-performance thin-layer chromatographic (HPTLC) method was developed and validated according to the protocol on Validation of Analytical Procedures: methodology, Veterinary International Cooperation on Harmonization for simultaneous determination of enrofloxacin and ciprofloxacin in broiler chicken tissues viz. liver, kidney, muscle-breast, muscle-thigh, and skin....
Fri, 11/03/2017 - 00:00
The tremorgenic paxilline (PAX), an indole-diterpene alkaloid mycotoxin, was recently detected as a natural contaminant of ergot of barley and rye. To check the possibility of a transfer of this mycotoxin into beer, a rapid and sensitive immunochemical method for the analysis of PAX in commercial bottled beer was developed. A straightforward sample preparation procedure could...
Fri, 11/03/2017 - 00:00
An interlaboratory proficiency test using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify food-associated bacteria was performed with 16 food microbiological laboratories, comprising ten authorities, four companies, and two academic institutions using either the Bruker Daltonics MALDI Biotyper system, the VITEK MS Ruo...
Wed, 11/01/2017 - 00:00
A polyvinylidene fluoride membrane-based dot immunoassay using nanobody (Nb) for rapid, qualitative, and visual detection of ochratoxin A (OTA) in cereals was developed. On the basis of optimal assay conditions, the cut-off level of this method assessed visually was 5 μg/kg for OTA, and the final results were obtained within 20 min. This method was simple with no time-...
Sun, 10/01/2017 - 00:00
Staphylococcus aureus is a major foodborne pathogen worldwide, and as little as 1 μg of staphylococcal enterotoxins (SEs) can cause food poisoning. Among SEs, enterotoxin A is the most common toxin that causes staphylococcal food poisoning. Hence, this work has developed a dual-labeled PCR-based immunofluorescent assay using anti-digoxigenin (...
Sun, 10/01/2017 - 00:00
A simple, sensitive, and selective analytical method was validated for the determination of mercury, cadmium, lead, arsenic, copper, iron, and zinc in fish muscle using microwave-induced plasma optical emission spectrometry (MIP OES) after acid digestion. The procedure for determining linear range, detection and quantification limits, selectivity, trueness, repeatability,...
Sun, 10/01/2017 - 00:00
A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (α-zearalanol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone) in animal-originated foods. The sample was first...
Fri, 09/01/2017 - 00:00
Current detection and characterization techniques for Salmonellae are time consuming, and rapid methods could benefit investigation and control of foodborne outbreaks. In this study, the potential of surface-enhanced Raman spectroscopy (SERS) in label-free detection and serotyping of Salmonella was evaluated. After immunomagnetic separation (IMS) and overnight culture, SERS...
Fri, 09/01/2017 - 00:00
A novel surface-enhanced Raman scattering (SERS) response emerging from aptamers, complementary DNA (cDNA), p-aminothiophenol (PATP), and Au nanorods (GNRs) for detection of foodborne pathogens sensitivity is proposed here. In the presence of Salmonella typhimurium (ST) and cDNA simultaneously, ST and cDNA would competitively combine with the S. typhimurium aptamer (STA),...
Fri, 09/01/2017 - 00:00
A simple and rapid method was developed for analysis of patulin in juice using ultra performance liquid chromatography coupled with tandem mass spectrometry. The samples were purified by an Oasis MAX 96-Well Plate. Chromatographic separation was performed on an ACQUITY UPLC® HSS T3 column with gradient elution using acetonitrile and water as mobile phase. Analytes was...
Tue, 08/01/2017 - 00:00
A vortex-assisted dispersive solid-phase microextraction (VA-d-μ-SPE) method for separation and preconcentration of polycyclic aromatic hydrocarbons (PAHs) using ionic liquid-modified metal–organic frameworks (IL-MIL-100(Fe)), prior to the measurement by gas chromatographic system equipped with a flame ionization detector (GC–FID) has been developed. The IL-MIL-100(Fe)...
Tue, 08/01/2017 - 00:00
Analytical technology-based solutions for automation of food processing according to the quality and safety factors are of great interest to food industry. In this study, a hyperspectral imaging system with a spectral range of 380–1000 nm was used for the detection of four levels of skin browning on button mushroom. Samples were stored at standard condition (3 ± 1 °C and 92...
Tue, 08/01/2017 - 00:00
A competitive indirect time-resolved fluoroimmunoassay (TRFIA) for detecting aflatoxin M1(AFM1) contamination in milk was developed, by using aflatoxin M1-bovineserum albumin conjugate, anti-AFM1 antibody, and Eu-labeled goat anti-rabbit antibody. To improve the sensitivity of the assay, the concentrations of the coating antigen and anti-AFM1 antibody were varied to optimize...
Tue, 08/01/2017 - 00:00
Analytical method for the determination of four flame retardants (FRs) from two groups was proposed. These groups included the brominated flame retardants (BFRs) 3,3′,5,5′-tetrabromobisphenol A (TBBPA) and 1,2,5,6,9,10-hexabromocyclododecane (HBCD); triester organophosphate flame retardants (OPFRs), ethylhexyl diphenyl phosphate (EHDP) and triphenyl phosphate (TPhP). Reversed...
Tue, 08/01/2017 - 00:00
Salmonella typhimurium is one of the most common causes of food-associated disease. A colorimetric nanosensor was developed to detect S. typhimurium which was based on the gold nanoparticles’ (GNPs) color change effect. Fe3O4 magnetic nanoparticles (MNPs) and GNPs were synthesized separately. Two complementary sequences of the S. typhimurium...
Tue, 08/01/2017 - 00:00
A nanoporous carbon prepared from metal−organic frameworks through high temperature treatment has been examined for the extraction of some endocrine disrupting chemicals (bisphenol A, 4-tert-octylphenol, nonylphenol, and 4-nonylphenol) from bottled juice samples. To obtain an optimum extraction performance, the main experimental parameters for the extraction including the...
Thu, 07/13/2017 - 00:00
The availability of analytical methods to detect the use of treatment, meet labeling requirements, and regulate international trade is a prerequisite for the approval of irradiation in the food sector. A single analytical method to be used to control all the types of foods is not currently available, and the distinction between irradiated and non-irradiated food in some cases...
Tue, 07/11/2017 - 00:00
Listeria monocytogenes (LM) is recognized as an opportunistic, foodborne pathogen that leads to the disease listeriosis. Although the incidence of listeriosis is low, listeriosis has a high mortality rate. LM can survive the most common stresses present during food processing steps, and it causes contamination in many food products. Consequently,...
Tue, 07/11/2017 - 00:00
Melamine is a nitrogen-rich compound (about 66%) whose fraudulent addition to foods aims to increase the apparent protein content. In 2008, melamine adulteration incidents occurred in China caused several deaths in humans from kidney failure and other health problems. This issue prompted private as well as governmental laboratories to develop several analytical methods in order...
Thu, 06/01/2017 - 00:00
Salmonella is a major food-borne pathogen in humans and a cause of local or systemic disease in animals. Therefore, rapid and reliable methods to detect these poultry-associated Salmonella serotypes are necessary for efficient control of Salmonella in poultry. The present study aimed to develop a real-time multiplex PCR (MqPCR) method to...
Mon, 05/01/2017 - 00:00
The quality and safety of food samples always require strict control. From an analytical perspective, many techniques can be used for this type of monitoring, one of which involves the application of laser pulses in laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). LA-ICP-MS has great advantages such as simplicity of operation, versatility, and high...
Mon, 05/01/2017 - 00:00
Rapid analysis of health hazardous materials is necessary to prevent foodborne illness in food control science. Bisphenol A (BPA) is one of the potentially hazardous materials that may potentially create adverse health effects, if its concentration level in food is not controlled. In this work, carbon paste electrode has been modified with CdO nanoparticles and ionic liquid (...

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