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Food Analytical Methods

Mon, 07/01/2019 - 00:00
With the increasing of the food safety awareness, the quality control of amaranth as the common food additives was significant for human’s physical health. The objective of this study was to establish a green, simple, and rapid fluorescence sensor for amaranth quantification detection. The nitrogen-doped graphene quantum dots (NGQDs) were synthesized under a friendly...
Mon, 07/01/2019 - 00:00
As patulin is a kind of mycotoxin commonly found in fruit products, it is of great importance to develop simple and accurate analytical methods for the sensitive detection of patulin (PAT). In this work, based on molecular imprinted polymer (MIP) and surface-enhanced Raman scattering (SERS) technique, a specific and sensitive PAT sensor was developed. To prepare the MIP-SERS...
Mon, 07/01/2019 - 00:00
Escherichia coli O157:H7 and Salmonella choleraesuis are two important foodborne pathogens that cause illnesses and even deaths. Rapid and convenient methods, such as immunochromatographic assays (ICAs), are useful for detecting these two pathogens. Herein, we developed a highly sensitive ICA for the simultaneous quantitative analysis of these two foodborne pathogens. Silver...
Sat, 06/01/2019 - 00:00
Organochlorines are common toxic contaminants in the food chain, especially in aquatic biota, thus leading the world population to undesired exposures. The chemical analyses of such trace pollutants may represent a challenge: if not properly conducted, they could suffer from great uncertainties. In the present work, a new candidate reference material of oyster tissue was...
Sat, 06/01/2019 - 00:00
Room temperature excitation-emission matrices (EEMs), recorded using front-face fluorescence spectroscopy, and combined with second-order multiway classification methods, were explored as potential tools for controlling the storage conditions of extra virgin olive oil samples (EVOO). Factors such as UV-irradiation, sunlight exposition and temperature up to 80 °C were studied...
Fri, 05/17/2019 - 00:00
Solid-phase extraction (SPE) was combined with dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) as a pretreatment technique for the simultaneous analysis of 11 organochlorine pesticides (OCPs) and 5 polychlorinated biphenyls (PCBs) in fish tissue. Gas chromatography with electron capture detector was used for the...
Fri, 05/17/2019 - 00:00
Tyramine is a naturally occurring monoamine compound produced from decarboxylation of the amino acid tyrosine by monoamine oxidase. Foodstuffs containing considerable amounts of tyramine include fish, fermented food, and other dairy products. High levels of tyramine in food samples cause toxic effects such as migraine, tachycardia, and vomiting. Therefore, sensitive methods are...
Thu, 05/16/2019 - 00:00
Sensitive detection of pathogenic bacteria is of great significance for food safety and public health. This study aimed to report a novel F0F1–ATPase aptasensor based on quantum dots (QDs) for quantitative detection of Vibrio parahaemolyticus. The fluorescent QDs were labeled onto the surface of chromatophores to act as an indicator. Aptamer target to V. parahaemolyticus was...
Sun, 05/12/2019 - 00:00
Salmonella is a type of serious foodborne pathogenic bacterium that threatens the food safety worldwide. In this study, a fluorescence probe-based recombinase polymerase amplification analytical method (exo RPA) was developed for the sensitive and specific detection of Salmonella enterica (S. enterica). The primers and probe for this method were designed based on the iroB gene...
Thu, 05/02/2019 - 00:00
The development and validation of a capillary electrophoresis–tandem mass spectrometry method for the determination of nine pesticides residues in corn samples is presented. The residues of thiabendazole, aminocarb, imazalil, atrazine, metazachlor, metoxuron, carbofuran, metosulam, and imazapyr have been extracted from corn samples using QuEChERS based extraction procedure. The...
Wed, 05/01/2019 - 00:00
Foodborne diseases arise from Listeria monocytogenes (L. monocytogenes) and Salmonella typhimurium (S. typhimurium) are global public health problems, influencing both developing and developed countries. Therefore, their rapid and accurate detection is of much concern in food safety and point-of-care diagnostics. In this study, a surface-enhanced Raman scattering (SERS)-based...
Wed, 05/01/2019 - 00:00
Sensitive identification and detection of melamine illegally added to food to increase false protein content is important for food safety monitoring because melamine can cause tissue injury or even death above the safety regulation level. Melamine can coordinate with two thymines to form stable T-melamine-T structures. On the basis of such a unique interaction, we have...
Wed, 05/01/2019 - 00:00
This study investigated the physicochemical characteristics and flavor attributes of navel oranges to determine the effect of low-dose phytosanitary irradiation and subsequent storage at 4 °C. Electronic sensing techniques such as E-tongue and E-nose successfully detected irradiated oranges based on their taste attributes and volatile patterns, respectively, even after...
Wed, 04/10/2019 - 00:00
Simultaneous extraction and preconcentration of five endocrine disrupting compounds including di-butyl phthalate, di-iso-nonyl phthalates, di-ethylhexyl phthalate, di-ethylhexyl adipate, and bisphenol A from cheese have been developed using pressurized liquid extraction combined with dispersive liquid–liquid extraction followed by high-performance liquid chromatography-diode...
Tue, 04/09/2019 - 00:00
In this study, electrospun graphene oxide–doped poly(acrylonitrile-co-maleic acid) nanofibers (E-spun-GO-PANCMA-NFs) were fabricated and investigated as novel adsorbent for solid phase extraction (SPE) of tetracycline antibiotics (TCs) in chicken samples. The as-synthesized E-spun-GO/PANCMA-NFs were characterized and then used as SPE adsorbent to combine with high-performance...
Wed, 04/03/2019 - 00:00
Reliable detection of foodborne pathogen is of great importance in guaranteeing food safety. In the present work, based on the multifunctional properties of platinum-coated gold nanorods (AuNR@Pt), we developed a dual-mode aptasensor based on inner filter effect (IFE) and color change strategies and applied it for Vibrio parahaemolyticus (V. parahaemolyticus) detection. Under...
Mon, 04/01/2019 - 00:00
Oligonucleotide aptamers as a new type of molecular recognition element, compared with conventional antibodies, have not only high affinity and specificity to target molecules but also many advantages such as a wide range of target molecule recognition, synthesis in vitro, and chemical stability. With deep concerns of human beings about food safety issues, various aptamer-...
Mon, 04/01/2019 - 00:00
Molecular imprinted polymers on copper nanoparticle–decorated vinyl-functionalized graphene (CuNPs@GR-MIPs) are fabricated. The copper nanoparticles (CuNPs) are synthesized by the reduction of diaminopropane copper complexes (DAPCu) using sodium borohydride as reducing agent. The synthesized CuNPs are successfully decorated on vinyl-functionalized graphene (V-fGR) on which...
Fri, 03/01/2019 - 00:00
An analytical method based on sugaring-out assisted liquid-liquid extraction (SULLE) and HPLC-fluorescence detection has been developed for the determination of bisphenol A (BPA) and bisphenol B (BPB) in royal jelly. Experimental parameters of SULLE including the extraction solvent, amount of phase separation agent, initial concentration of acetonitrile (ACN), and sample size...
Fri, 03/01/2019 - 00:00
A gold nanoparticle/reduced graphene oxide composite electrode (AuNP/rGO) was fabricated for the determination of the main bioamines and their precursor amino acids in beer based on a miniaturized capillary electrophoresis with amperometric detection system (mini-CE-AD). The surface morphology and structure of the synthetic AuNP/rGO material were observed by scanning electron...
Fri, 03/01/2019 - 00:00
A novel, sensitive, and rapid analytical method for analyzing valifenalate residue in grape, vegetables (tomato, lettuce, eggplant, potato), and soil was established and validated using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method by ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The determination...
Fri, 03/01/2019 - 00:00
Acrylamide, a “possible carcinogen” compound, has been subjected to analytical method development and evaluation in carbohydrate-rich cookie samples in this research. Cookies with different formula and consequently different acrylamide content were evaluated using microextraction method coupled with gas chromatography-mass spectrometry. Xanthydrol was selected as an efficient...
Tue, 02/12/2019 - 00:00
Cu-based metal-organic frameworks modified by silver (Ag/Cu-MOFs) were in situ fabricated through a one-step direct synthesis. The as-prepared mixed-node MOFs were modified on glassy carbon (GC) electrode, which was utilized as a voltammetric sensor for probing malachite green. Compared with the bare GC electrode, the introduced Ag/Cu-MOF film on the electrode surface...
Fri, 02/01/2019 - 00:00
In this study, an immunology-based assay that employed specific monoclonal antibodies binding with somatic or flagella antigens of Salmonella enterica subsp. enterica was performed. As this pathogen is one of the most important bacterial species responsible for foodborne outbreaks, its detection in food by rapid and easy methods is properly suitable. After a first screening...
Fri, 02/01/2019 - 00:00
In this study, a combination of modified quick easy cheap effective rugged and safe extraction and dispersive liquid–liquid microextraction has been proposed for the extraction and preconcentration of some widely used pesticides (diazinon, chlorpyrifos, penconazole, oxadiazon, and diniconazole) from fruit and vegetable samples prior to their determination by gas...
Fri, 02/01/2019 - 00:00
The novel and highly sensitive methods for individual determination of two food azo dyes Amaranth (AM, E 123) and Allura Red AC (AR, E 129) in the commercial beverages were successfully developed on two types of silver solid amalgam electrodes, namely mercury meniscus modified (m-AgSAE) and liquid mercury free polished (p-AgSAE) amalgam electrodes, using differential pulse...
Fri, 02/01/2019 - 00:00
The occurrence of antibiotic residues in milk constitutes a potential risk to the health of consumers. The present study describes the optimisation and validation of a high-performance liquid chromatographic (HPLC) method for the simultaneous determination of sulphadiazine (SDZ), sulphamethoxazole (SMX), oxytetracycline (OTC), doxycycline (DOX), tetracycline (TC),...
Fri, 02/01/2019 - 00:00
In this study, a rapid and sensitive method that combines ethidium bromide monoazide (EMA) staining with real-time loop-mediated isothermal amplification (Rti-LAMP) assay was tested in detecting and evaluating viable but non-culturable state (VBNC) Escherichia coli (E. coli) O157:H7 induced by low temperature. When 1.7 × 104 CFU/ml of E. coli O157:H7 was 3 cycles of freeze-...
Fri, 02/01/2019 - 00:00
In this work, a rapid, simple, and environmentally friendly method has been proposed for direct supramolecular microextraction of four organophosphate insecticides (ethion, phosalone, diazinon, chlorpyrifos) and an isothiazolidine acaricide (hexythiazox) in agricultural product samples prior to their determination by high-performance liquid chromatography-ultraviolet...
Fri, 02/01/2019 - 00:00
This paper investigated a new detection method for food pathogenic bacteria Vibrio parahaemolyticus based on surface-enhanced Raman scattering (SERS). Nanostructures consisting of Aptamer-Au nanoparticles-polydimethylsiloxane (Apt-Au-PDMS film) were firstly used as SERS substrate. Aptamers were immobilized on the functionalized PDMS film covered with Au nanoparticles (AuNPs)...
Sun, 01/06/2019 - 00:00
There is a general consensus that the consumption of organic food can contribute to a healthy diet; nevertheless, large-scale production of organic food is not an easy task since it requires intense care due to the number of pests, fungi, and diseases that can wipe out an entire crop. Researchers evaluating food quality are often concerned with the use of pesticides,...
Fri, 01/04/2019 - 00:00
Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M1 (AFM1), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B1 (FB1), were selected as model...
Thu, 01/03/2019 - 00:00
Hydrogen sulfide (H2S) has been involved in many foodstuffs and beverages. On-site measuring the H2S level in food samples was beneficial to the concerns about food safety and food quality. Herein, we rationally constructed a novel fluorescent probe to track the H2S levels in various food samples. The optical experiments indicated that the probe displayed highly selective and...
Tue, 01/01/2019 - 00:00
In this study, we aimed to determine the characteristics of residual pesticides of pyraclostrobin (22.9% emulsifiable concentrate [EC]) and trifloxystrobin (22% suspension concentrate [SC]) in red pepper powder and to determine the processing and reduction factors in red pepper powder during processing. The pesticides were sprayed twice onto red pepper cultivated in an open...
Tue, 01/01/2019 - 00:00
In this study, quick, easy, cheap, effective, rugged, and safe (QuEChERS) method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied and optimized in determination of 34 commonly used pesticides in black pepper. Two cleanup approaches including dispersive solid-phase extraction (d-SPE) and dispersive solid liquid extraction (d-SLE) were...
Tue, 01/01/2019 - 00:00
The objective of this study is to introduce the usefulness of the liquid fingerprinting technology in spotting potential health hazards in foodstuff, exemplifying milk. The presented method relies on the use of long lifetime photoluminescent europium chelate and individual cross-reactive modulators in an array form. Here, we demonstrate the potency of liquid fingerprinting...
Tue, 01/01/2019 - 00:00
Patulin is a mycotoxin that exhibits a number of toxic effects in animals. The main sources of patulin intake in human diet that was shown for EU consumers are apple juice and nectar and for this reason, apple-based food is most often monitored for this mycotoxin. However, the presence of patulin in other fruits, including stone fruits, and soft fruits has been reported as...
Sat, 12/01/2018 - 00:00
An extraction method for simultaneous determination of aflatoxins (AFLAs) G2, G1, B2, and B1 in cornmeal, based on vortex-assisted matrix solid-phase dispersion (MSPD) and high-performance liquid chromatography (HPLC) with fluorescence detection was optimized by a central composite design, validated and applied. Multivariate analysis was performed to evaluate the effect of...
Sat, 12/01/2018 - 00:00
New multiresidue methods for identification and quantification of over 131 pesticides in wine and grapes have been developed. The analyses were based on gas chromatography with microelectron capture and nitrogen–phosphorus detection (GC-μECD/NPD). Two different extraction protocols (QuEChERS-citrate buffer and unbuffered methods) for wine samples were described. A full...
Sat, 12/01/2018 - 00:00
New fast and efficient analytical method based on QuEChERS followed by dispersive liquid-liquid microextraction (DLLME) was developed for extraction and pre-concentration of residues of ethion and bifenthrin insecticide/acaricides in palm date matrix. GC-MS was used for quantification of the pesticides. The developed method was validated according to the European regulations...
Sat, 12/01/2018 - 00:00
Phthalic acid esters (PAEs) have become an important food safety concern due to their lipophilic properties and propensity to accumulate in adipose tissue in edible fish. In this study, a simple, sensitive, and accurate analytical method was successfully established for simultaneous determination of 19 PAEs in fish samples using gas chromatography coupled with tandem mass...
Sat, 12/01/2018 - 00:00
The presence of foodborne pathogens is one of the leading causes of food safety incidents; hence, early monitoring of the status of microbial contamination is a permanent concern. Here, a rapid and sensitive method for detection of Salmonella typhimurium was firstly developed by combining heavy water (D2O) labeling and Raman microspectroscopy to measure bacterial metabolic...
Thu, 11/01/2018 - 00:00
Korean citrus fruits (Jinjihyang and Chunggyun) were treated with electron beam (e-beam) at phytosanitary irradiation doses of 0, 0.4, and 1 kGy, and their irradiation status was screened after storage for 0, 10, and 20 days. Because of the inherent limitations of photo-stimulated luminescence (PSL), the first PSL measurement (PSL1) was not sufficient to distinguish the...
Thu, 11/01/2018 - 00:00
The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v...
Thu, 11/01/2018 - 00:00
A structure of polyurethane micelle/Ag nanoparticle (Ag NP) cluster was fabricated as surface-enhanced Raman scattering (SERS) substrate for in situ extracting organic compounds on the surface of different matrixes, in which polyurethane micelles acted as the host material to capture target molecules and stabilize nanoparticle cluster. This method provided stable aqueous...
Thu, 11/01/2018 - 00:00
A novel, sensitive, and selective electrochemical sensor has been fabricated for the determination of 1-naphthyl methylcarbamate (carbaryl) as an important pesticide. Three-dimensional graphene (3DG) was synthesized by using thiourea as the crosslinking agent that simultaneously caused impregnation of sulfur and nitrogen functional groups onto the graphene. Graphene doped...
Thu, 11/01/2018 - 00:00
Staphylococcus aureus (S. aureus) is an important food-borne pathogen which poses a severe threat to public health worldwide. Rapid detection of S. aureus with high sensitivity is of particular importance for food safety. In this study, a novel single primer isothermal amplification (SPIA) method was established to detect S. aureus in food, targeting the accessory gene...
Thu, 11/01/2018 - 00:00
A simple, fast and ecological analytical method using a semi-automatic fat extractor and HPLC-FLD (fluorescence detection) for determination of polycyclic aromatic hydrocarbon markers i.e. benzo(a)anthracene (BaA), chrysene (Chr), benzo(a)pyrene (BaP) and benzo(b)fluoranthene (BbF) in cocoa butter has been validated. Validation’s procedure performed out in concordance with...
Thu, 11/01/2018 - 00:00
A sensitive, rapid, and efficient analytical method based on vortex-assisted liquid–liquid extraction combined with air-assisted liquid–liquid microextraction using high performance liquid chromatography-variable wavelength detection has been developed for the extraction and determination of bisphenol A and bisphenol B in canned doogh samples. In this method, the analytes are...
Mon, 10/01/2018 - 00:00
In this study, a simple and high-throughput multiresidue pesticide analysis method based on the quick, easy, cheap, effective, rugged, safe (QuEChERS) extraction combined with gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) and ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) is presented for the determination of...

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