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Food Analytical Methods

Fri, 06/01/2018 - 00:00
A multi-pesticide metabolite screening method using liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-Tof-MS) was developed for the detection of pesticide metabolites in fruit and vegetable samples. Based on a retrospectively created accurate mass compound database, a suspect screening approach was established for pesticides of high concern applied to...
Fri, 06/01/2018 - 00:00
The present study proposes an alternative analytical method for the determination of acrylamide in potato chips, using 2-naphthalenethiol as derivatization reagent followed by a HPLC separation with fluorescence detection to be used as a routine analytical procedure in the potato chip industry. The proposed method requires a relatively low-cost instrumentation compared to the...
Fri, 06/01/2018 - 00:00
Microcystins (MCs) pose a major threat to shellfish supplies, and humans that have been in contact with or consume contaminated products. To decrease health risks to the public, an efficient and accurate method for the synchronously determination of seven harmful MCs in shellfish samples was developed by employing a hydrophile lipophile balance/polydimethylsiloxane (HLB/PDMS...
Fri, 06/01/2018 - 00:00
Consumer and environmental groups have increasing concern about heavy metal contaminants and residual catalysts in plastics inducing negative human and environmental impacts. Regulations of heavy metals in food-contact plastics seek to reduce these risks by establishing maximum allowable threshold levels. However, traditional quantification methods of heavy metal...
Fri, 06/01/2018 - 00:00
A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation was developed for the simultaneous determination of 12 perfluorocarboxylic acids (PFCAs) and 7 perfluorosulfonic acids (PFSAs) in the muscle of shrimp, shellfish, and fish. A modified QuEChERS procedure was applied to the...
Fri, 06/01/2018 - 00:00
Glyphosate is the most widely used herbicide at the moment. It presents a broad spectrum of action, hence its use for many different crops. Regulatory agencies have constantly mentioned the low hazard potential to mammals. However, the International Agency for Research on Cancer concluded in 2015 that glyphosate is “probably carcinogenic to humans.” For this reason, it is...
Wed, 05/02/2018 - 00:00
Staphylococcus aureus (S. aureus) is of great importance and is a leading cause of food poisoning, which is a public health concern in terms of the frequency and seriousness of the disease. In the present study, RPA and real-time RPA assays were developed and validated to detect S. aureus with high sensitivity and specificity by targeting the nuc gene for the first time. The...
Tue, 05/01/2018 - 00:00
An analytical method based on liquid-liquid extraction combined with solid-phase extraction and isotope dilution-ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was well developed for simultaneous determination of aflatoxin B1 (AFB1), bisphenol A (BPA), and 4-nonylphenol (4-NP) in peanut oil. After adding isotope internal standards, the...
Tue, 05/01/2018 - 00:00
A group of methods were developed for the determination of etofenprox, kresoxim-methyl, cyprodinil, azoxystrobin, and pyrimethanil residues in animal-derived food (milk, egg, pig liver, pig fat, pork, and chicken) by using QuEChERS (quick, easy, cheap, effective, rugged, and safe)-gas chromatography/mass spectrometry (GC/MS). With acetonitrile as the extraction solvent, the...
Tue, 04/03/2018 - 00:00
Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption, thereby necessitating their effective monitoring and control. Antibody-based immunoassays provide efficient and cheap methods for screening this mycotoxin. To achieve high sensitivity and avoid enzymatic reaction, a...
Mon, 04/02/2018 - 00:00
The use of clenbuterol (CLE) as a growth-promoting agent in livestock is banned in the USA, European Union, and Mexico. Nevertheless, different studies have reported the presence of this agent in meat products, potentially causing intoxication in consumers. In this study, ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) is proposed for...
Sun, 04/01/2018 - 00:00
Determination of minerals is important for quality control of animal foods and sample preparation is a critical step to obtain fast, accurate, and reliable results. Extraction using dilute acid solutions is an interesting alternative due to its simplicity, softness, and safety. However, most extraction procedures are performed in batch mode, which generally is more time...
Thu, 03/01/2018 - 00:00
An accurate, rapid, and reliable multiresidue QuEChERS method based on gas chromatography coupled with tandem mass spectrometry was developed for the determination of 235 pesticides in challenging, dry, complex herb matrices (Centaurea cyanus L., Matricaria chamomilla L., Thymus vulgaris L.). Sample mass and the type of cleanup sorbent used to estimate the procedure’s...
Thu, 03/01/2018 - 00:00
A method of hollow fiber–stir bar sorptive extraction (HF–SBSE) coupled with gas chromatography–mass spectrometry was proposed to explore the migration of eight organochlorine pesticide residues in environmental and biological matrices. HF–SBSE was a configuration for solid–phase microextraction purposes, combining the features of SBSE and hollow fiber membrane extraction...
Thu, 03/01/2018 - 00:00
Benzo[a]pyrene (BaP), a kind of polycyclic aromatic hydrocarbon, has great potential hazards to the ecosystem and human health. In this study, based on the preparation of BaP immunogen and coating antigen, a specific polyclonal antibody targeting BaP was generated, and a highly sensitive real-time immuno-PCR assay using the biotin-streptavidin system (BA-IPCR) was developed...
Thu, 03/01/2018 - 00:00
A quantum-dot-based immunochromatographic assay (QICA) and a fluorescence quenching immunochromatographic assay (FQICA) were developed to visually detect bisphenol A (BPA). The proposed QICA and FQICA were based on traditional immune competition and on fluorescence resonance energy transfer (FRET) from fluorescence donors (quantum dots) to fluorescence acceptors (colloidal...
Thu, 03/01/2018 - 00:00
A fast and simple sample preparation method based on dispersive liquid–liquid microextraction (DLLME) and ultrasonic-assisted extraction (UAE) was proposed in order to determine aflatoxin B1 (AFB1) in eggs. In this study, AFB1 was first extracted from matrixes of egg samples with acetonitrile/water 80:20 (V/V), then its eluents were utilized as dispersant of the followed UAE-...
Thu, 03/01/2018 - 00:00
In this research, for the first attempt, we successfully determined seven polycyclic aromatic hydrocarbons (PAHs) in various coffee samples using microwave-assisted extraction and dispersive liquid-liquid microextraction (MAE-DLLME) coupled with gas chromatography-mass spectrometry (GC-MS). The effects of most important variables in microextraction step were investigated and...
Thu, 02/01/2018 - 00:00
The first aim of this study was to create, test, and apply a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method, based on acetonitrile extraction, for simultaneous determination of pesticide residues in green chili pepper (fresh and dried forms). There is a group of 86 repeatedly detected pesticides in the agricultural food commodity in Al-Qassim region, Saudi...
Thu, 02/01/2018 - 00:00
A solid-phase extraction (SPE) combined with high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method was developed to determine five phenols in grease-rich food (i.e., hotpot seasoning). Sample added with isotope internal standards bisphenol A-d 4 (BPA-d 4) and 4-n-nonylphenol-d 4 (4...
Thu, 02/01/2018 - 00:00
This study describes the development of a novel reproducible molecularly imprinted quartz crystal microbalance (QCM) sensor for the accurate and sensitive analysis of the residue of enrofloxacin (ENRO) in animal-derived foods. This proposed sensor was easily fabricated by directly immobilizing molecularly imprinted polymer (MIP) of ENRO on the surface of a QCM Au chip, which...
Thu, 02/01/2018 - 00:00
The vine produces de novo compounds with small molecules, called phytoalexins, as defense response to oxidative stress, microbial infections, and UV irradiation. Such bioactive substances are trans-resveratrol and quercetin. In this study, a rapid HPLC method was developed and validated for simultaneous quantification of both phenolic compounds and was applied for their...
Thu, 02/01/2018 - 00:00
A rapid, simple, and efficient method was described for the simultaneous determination of three common antibiotic residues including erythromycin, tetracycline, and chloramphenicol in raw milk. A molecular imprinting technique combined with solid-phase extraction (SPE) was used to pretreat the test samples and then simultaneously detected with HPLC. The whole process, only...
Thu, 02/01/2018 - 00:00
A simple sensor ordered mesoporous carbon/glassy carbon electrode (OMC/GCE) is developed in this paper for melamine (Mel) detection, which is based on conversion of Mel from non-electroactive Mel to electroactive Cu-Mel complex under presence of copper ions in 0.1 M pH 9.0 borate buffer containing 20% methanol (v/v) as supporting electrolyte at the surface of OMC modified...
Mon, 01/01/2018 - 00:00
The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental conditions. Under selected conditions (using melamine as the additive), both L-...
Tue, 12/19/2017 - 00:00
Protection of the quality products and particularly Protected Designation of Origin (PDO)/Protected Geographical Indication (PGI) foods is a strategic issue in the EU economy, in terms of protection of market competition and safety. Having reliable tools for the assessment of key parameters useful for the identification of authenticity and/or frauds is therefore of main...
Tue, 12/19/2017 - 00:00
A porous β-cyclodextrin polymer (P-CDP) was used as a new sorbent for simultaneous solid-phase extraction (SPE) and determination of bisphenol A (BPA), bisphenol F (BPF), and bisphenol AF (BPAF). The P-CDP was obtained via the cross-linking of β-cyclodextrin with tetrafluoroterephthalonitrile, and then was characterized by FT-IR spectroscopy, scanning electron microscopy,...
Sun, 12/17/2017 - 00:00
Volatiles are critical for real and perceived quality of mussels. In the present study, we determined, for the very first time, the characteristic volatiles of fresh Mediterranean mussels (Mytilus galloprovincialis) and their variation during 4 days of storage at 6.0 ± 0.5 °C. During this time, volatile organic compounds (VOCs) were monitored using SPME-GC-MS. Twenty-seven VOCs...
Sun, 12/17/2017 - 00:00
Alternariol (AOH) is one of the major mycotoxins produced by various species of Alternaria fungi. Natural occurrences of AOH have been reported in various foods, including fruits; processed fruit products such as apple juice, tomato products; wheat and other grains; oilseeds and products thereof, such as sunflower seeds, oilseed rape meal, and flax seed/linseed; and pecans. In...
Tue, 12/12/2017 - 00:00
Hydrogen sulfide (H2S) has recently been recognized as the third endogenous gaseous signaling molecule besides NO and CO. At the same time, H2S is responsible for an important proportion of faulty wines with potentially large economic losses. Thus, the H2S in wine not only contributes negatively to wine aroma but also faces a problem of food safety. So an efficient and...
Thu, 12/07/2017 - 00:00
Acrylamide (ACR) is a potential carcinogen and is found in thermally processed foods such as potato chips, biscuits, baby foods, coffee, etc. In this paper, l-cysteine-capped Mn-doped ZnS quantum dots (QDs) as phosphorescent probes were used for the determination of ACR. This method based on quenching of the phosphorescence intensity of the QDs with the interaction of ACR. Room...
Wed, 12/06/2017 - 00:00
In this study, a rapid and sensitive method of real-time loop-mediated isothermal amplification (Rti-LAMP) assays was developed for quantification and discrimination of viable and heat-killed E. coli O157:H7 cells treated with low concentration of ethidium bromide monoazide (EMA). Four micrograms per milliliter of EMA was chosen as the optimal concentration which did not...
Sun, 12/03/2017 - 00:00
Pyrrolizidine alkaloids (PAs) are secondary metabolites produced by plants as a chemical defense against herbivores. Plants containing PAs are widely distributed in almost all geographical regions posing a risk of honey contamination. To provide safety of honey and decrease the potential risk for the consumers, a sensitive method based on gas chromatography-mass spectrometry...
Fri, 12/01/2017 - 00:00
A voltammetric method for fast and high sensitive determination of Bisphenol A (BPA) using glassy carbon electrode (GC) modified with carbon black nanoparticles (CB) was developed. Cyclic voltammetry study in the 0.1 M phosphates of pH = 7.0 gave single anodic peak at 578 mV. Adsorption-controlled oxidation of BPA was found to be irreversible with the participation of two...
Fri, 12/01/2017 - 00:00
In this study, a core–shell magnetic dummy molecularly imprinted polymer (MDMIP) was prepared with a novel and simple method. The imprinting layer was synthesized directly on the surface of Fe3O4 by only one step, which avoided further modification on the exterior of magnetic core. 4,4′-Dihydroxybiphenyl (DDBP) was used as dummy template instead of bisphenol A (BPA) to...
Fri, 12/01/2017 - 00:00
Modified QuEChERS method combined with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed and validated to determine pesticide residues in dried cocoa beans. Extraction efficiency of matrix hydration effect on 19 pesticide residues was studied and optimized using 23 full factorial design with percentage recoveries as the response variable. The optimized...
Fri, 12/01/2017 - 00:00
The application of a simple, fast, and cheap sample pre-concentration technique based on the dispersive magnetic Fe3O4 nanoparticle solid-phase extraction has been developed for extraction and determination of aflatoxins B1, B2, G1, and G2 in nut and cereals. Aflatoxins were extracted from samples with a mixture of methanol–water 8:2 (v/v) and then by magnetic Fe3O4...
Fri, 12/01/2017 - 00:00
An acryloyl β-cyclodextrin (A-β-CD) monolithic column for solid-phase microextraction (SPME) and determination of carbofuran and carbaryl by high-performance liquid chromatography (HPLC) have been prepared through a “one-step” polymerization method. The synthesis conditions, including the volume of cross-linker, the ratio and volume of mixed porogenic solvent consisted of...
Fri, 12/01/2017 - 00:00
Vanilla extracts are widely used as flavoring ingredients in foods, beverages, perfumes, and pharmaceuticals. Due to the high cost of producing high-quality natural extracts, artificial flavorings, such as ethyl vanillin and coumarin, are often used. For food safety and to ensure high-quality products, it is important to differentiate between natural and artificial extracts....
Tue, 11/07/2017 - 00:00
Conventional methods to detect Salmonella in milk generally take a long time and are significantly laborious besides requiring the use of different media and reagents. Thus, this research aimed to offer an alternative methodology based on near-infrared spectroscopy (NIRS) to discriminate the presence of Salmonella in milk. For this purpose, different milk samples, contaminated...
Mon, 11/06/2017 - 00:00
A high-performance thin-layer chromatographic (HPTLC) method was developed and validated according to the protocol on Validation of Analytical Procedures: methodology, Veterinary International Cooperation on Harmonization for simultaneous determination of enrofloxacin and ciprofloxacin in broiler chicken tissues viz. liver, kidney, muscle-breast, muscle-thigh, and skin....
Fri, 11/03/2017 - 00:00
The tremorgenic paxilline (PAX), an indole-diterpene alkaloid mycotoxin, was recently detected as a natural contaminant of ergot of barley and rye. To check the possibility of a transfer of this mycotoxin into beer, a rapid and sensitive immunochemical method for the analysis of PAX in commercial bottled beer was developed. A straightforward sample preparation procedure could...
Fri, 11/03/2017 - 00:00
An interlaboratory proficiency test using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify food-associated bacteria was performed with 16 food microbiological laboratories, comprising ten authorities, four companies, and two academic institutions using either the Bruker Daltonics MALDI Biotyper system, the VITEK MS Ruo...
Wed, 11/01/2017 - 00:00
A polyvinylidene fluoride membrane-based dot immunoassay using nanobody (Nb) for rapid, qualitative, and visual detection of ochratoxin A (OTA) in cereals was developed. On the basis of optimal assay conditions, the cut-off level of this method assessed visually was 5 μg/kg for OTA, and the final results were obtained within 20 min. This method was simple with no time-...
Sun, 10/01/2017 - 00:00
Staphylococcus aureus is a major foodborne pathogen worldwide, and as little as 1 μg of staphylococcal enterotoxins (SEs) can cause food poisoning. Among SEs, enterotoxin A is the most common toxin that causes staphylococcal food poisoning. Hence, this work has developed a dual-labeled PCR-based immunofluorescent assay using anti-digoxigenin (...
Sun, 10/01/2017 - 00:00
A simple, sensitive, and selective analytical method was validated for the determination of mercury, cadmium, lead, arsenic, copper, iron, and zinc in fish muscle using microwave-induced plasma optical emission spectrometry (MIP OES) after acid digestion. The procedure for determining linear range, detection and quantification limits, selectivity, trueness, repeatability,...
Sun, 10/01/2017 - 00:00
A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (α-zearalanol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone) in animal-originated foods. The sample was first...
Fri, 09/01/2017 - 00:00
Current detection and characterization techniques for Salmonellae are time consuming, and rapid methods could benefit investigation and control of foodborne outbreaks. In this study, the potential of surface-enhanced Raman spectroscopy (SERS) in label-free detection and serotyping of Salmonella was evaluated. After immunomagnetic separation (IMS) and overnight culture, SERS...
Fri, 09/01/2017 - 00:00
A novel surface-enhanced Raman scattering (SERS) response emerging from aptamers, complementary DNA (cDNA), p-aminothiophenol (PATP), and Au nanorods (GNRs) for detection of foodborne pathogens sensitivity is proposed here. In the presence of Salmonella typhimurium (ST) and cDNA simultaneously, ST and cDNA would competitively combine with the S. typhimurium aptamer (STA),...
Fri, 09/01/2017 - 00:00
A simple and rapid method was developed for analysis of patulin in juice using ultra performance liquid chromatography coupled with tandem mass spectrometry. The samples were purified by an Oasis MAX 96-Well Plate. Chromatographic separation was performed on an ACQUITY UPLC® HSS T3 column with gradient elution using acetonitrile and water as mobile phase. Analytes was...

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