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Food Analytical Methods

Sat, 12/01/2018 - 00:00
An extraction method for simultaneous determination of aflatoxins (AFLAs) G2, G1, B2, and B1 in cornmeal, based on vortex-assisted matrix solid-phase dispersion (MSPD) and high-performance liquid chromatography (HPLC) with fluorescence detection was optimized by a central composite design, validated and applied. Multivariate analysis was performed to evaluate the effect of...
Sat, 12/01/2018 - 00:00
New multiresidue methods for identification and quantification of over 131 pesticides in wine and grapes have been developed. The analyses were based on gas chromatography with microelectron capture and nitrogen–phosphorus detection (GC-μECD/NPD). Two different extraction protocols (QuEChERS-citrate buffer and unbuffered methods) for wine samples were described. A full...
Sat, 12/01/2018 - 00:00
New fast and efficient analytical method based on QuEChERS followed by dispersive liquid-liquid microextraction (DLLME) was developed for extraction and pre-concentration of residues of ethion and bifenthrin insecticide/acaricides in palm date matrix. GC-MS was used for quantification of the pesticides. The developed method was validated according to the European regulations...
Sat, 12/01/2018 - 00:00
Phthalic acid esters (PAEs) have become an important food safety concern due to their lipophilic properties and propensity to accumulate in adipose tissue in edible fish. In this study, a simple, sensitive, and accurate analytical method was successfully established for simultaneous determination of 19 PAEs in fish samples using gas chromatography coupled with tandem mass...
Sat, 12/01/2018 - 00:00
The presence of foodborne pathogens is one of the leading causes of food safety incidents; hence, early monitoring of the status of microbial contamination is a permanent concern. Here, a rapid and sensitive method for detection of Salmonella typhimurium was firstly developed by combining heavy water (D2O) labeling and Raman microspectroscopy to measure bacterial metabolic...
Thu, 11/01/2018 - 00:00
Korean citrus fruits (Jinjihyang and Chunggyun) were treated with electron beam (e-beam) at phytosanitary irradiation doses of 0, 0.4, and 1 kGy, and their irradiation status was screened after storage for 0, 10, and 20 days. Because of the inherent limitations of photo-stimulated luminescence (PSL), the first PSL measurement (PSL1) was not sufficient to distinguish the...
Thu, 11/01/2018 - 00:00
The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v...
Thu, 11/01/2018 - 00:00
A structure of polyurethane micelle/Ag nanoparticle (Ag NP) cluster was fabricated as surface-enhanced Raman scattering (SERS) substrate for in situ extracting organic compounds on the surface of different matrixes, in which polyurethane micelles acted as the host material to capture target molecules and stabilize nanoparticle cluster. This method provided stable aqueous...
Thu, 11/01/2018 - 00:00
A novel, sensitive, and selective electrochemical sensor has been fabricated for the determination of 1-naphthyl methylcarbamate (carbaryl) as an important pesticide. Three-dimensional graphene (3DG) was synthesized by using thiourea as the crosslinking agent that simultaneously caused impregnation of sulfur and nitrogen functional groups onto the graphene. Graphene doped...
Thu, 11/01/2018 - 00:00
Staphylococcus aureus (S. aureus) is an important food-borne pathogen which poses a severe threat to public health worldwide. Rapid detection of S. aureus with high sensitivity is of particular importance for food safety. In this study, a novel single primer isothermal amplification (SPIA) method was established to detect S. aureus in food, targeting the accessory gene...
Thu, 11/01/2018 - 00:00
A simple, fast and ecological analytical method using a semi-automatic fat extractor and HPLC-FLD (fluorescence detection) for determination of polycyclic aromatic hydrocarbon markers i.e. benzo(a)anthracene (BaA), chrysene (Chr), benzo(a)pyrene (BaP) and benzo(b)fluoranthene (BbF) in cocoa butter has been validated. Validation’s procedure performed out in concordance with...
Thu, 11/01/2018 - 00:00
A sensitive, rapid, and efficient analytical method based on vortex-assisted liquid–liquid extraction combined with air-assisted liquid–liquid microextraction using high performance liquid chromatography-variable wavelength detection has been developed for the extraction and determination of bisphenol A and bisphenol B in canned doogh samples. In this method, the analytes are...
Thu, 11/01/2018 - 00:00
Korean citrus fruits (Jinjihyang and Chunggyun) were treated with electron beam (e-beam) at phytosanitary irradiation doses of 0, 0.4, and 1 kGy, and their irradiation status was screened after storage for 0, 10, and 20 days. Because of the inherent limitations of photo-stimulated luminescence (PSL), the first PSL measurement (PSL1) was not sufficient to distinguish the...
Thu, 11/01/2018 - 00:00
The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v...
Thu, 11/01/2018 - 00:00
A structure of polyurethane micelle/Ag nanoparticle (Ag NP) cluster was fabricated as surface-enhanced Raman scattering (SERS) substrate for in situ extracting organic compounds on the surface of different matrixes, in which polyurethane micelles acted as the host material to capture target molecules and stabilize nanoparticle cluster. This method provided stable aqueous...
Thu, 11/01/2018 - 00:00
A novel, sensitive, and selective electrochemical sensor has been fabricated for the determination of 1-naphthyl methylcarbamate (carbaryl) as an important pesticide. Three-dimensional graphene (3DG) was synthesized by using thiourea as the crosslinking agent that simultaneously caused impregnation of sulfur and nitrogen functional groups onto the graphene. Graphene doped...
Thu, 11/01/2018 - 00:00
Staphylococcus aureus (S. aureus) is an important food-borne pathogen which poses a severe threat to public health worldwide. Rapid detection of S. aureus with high sensitivity is of particular importance for food safety. In this study, a novel single primer isothermal amplification (SPIA) method was established to detect S. aureus in food, targeting the accessory gene...
Thu, 11/01/2018 - 00:00
A simple, fast and ecological analytical method using a semi-automatic fat extractor and HPLC-FLD (fluorescence detection) for determination of polycyclic aromatic hydrocarbon markers i.e. benzo(a)anthracene (BaA), chrysene (Chr), benzo(a)pyrene (BaP) and benzo(b)fluoranthene (BbF) in cocoa butter has been validated. Validation’s procedure performed out in concordance with...
Thu, 11/01/2018 - 00:00
A sensitive, rapid, and efficient analytical method based on vortex-assisted liquid–liquid extraction combined with air-assisted liquid–liquid microextraction using high performance liquid chromatography-variable wavelength detection has been developed for the extraction and determination of bisphenol A and bisphenol B in canned doogh samples. In this method, the analytes are...
Mon, 10/01/2018 - 00:00
In this study, a simple and high-throughput multiresidue pesticide analysis method based on the quick, easy, cheap, effective, rugged, safe (QuEChERS) extraction combined with gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) and ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) is presented for the determination of...
Mon, 10/01/2018 - 00:00
Red meat is an important source of nutrients and plays a significant role in human diet. With the development of people’s living standard and relative change of dietary structure in recent years, people propose more requirements for meat. Quality, safety, and classification are three crucial themes related with meat and they are important issues for consumers, retailers, as...
Mon, 10/01/2018 - 00:00
In this work, g-C3N4 was successfully fabricated by direct pyrolysis of the melamine, and then without any modification, was used to prepare g-C3N4/graphite pencil electrode. The maximum current at this working electrode was depended significantly by the pH value; thus, the g-C3N4/tartrazine interaction is pH-dependent and the best pH was obtained in an acidic medium at pH 2....
Mon, 10/01/2018 - 00:00
The identification of transformation products (TPs) of pesticides in food is a crucial task difficult to tackle, due to the lack of standards. In this work, we present a novel methodology to synthesize five main TP standards of the insecticide chlorpyrifos (CPF) and to investigate their occurrence in selected fruits and spices. TPs were electrochemically (EC) synthesized...
Mon, 10/01/2018 - 00:00
An easy and quick sample pretreatment method, air-assisted dispersive liquid–liquid microextraction (AA-DLLME), has been introduced for the isolation and enrichment of aflatoxins B1, B2, G1, and G2 from rice samples prior to HPLC-FLD analysis. The method has also been compared with immunoaffinity column clean-up (IAC) method. In the case of AA-DLLME, the dispersion of...
Sat, 09/01/2018 - 00:00
The detection of polycyclic aromatic hydrocarbons (PAHs) and their metabolites in food and in agricultural sources is an important research objective due to the PAHs’ known persistence, carcinogenicity, and toxicity. PAHs have been found in the milk of lactating cows and in the leaves and stems of plants grown in PAH-contaminated areas, thereby making their way into both cow...
Sat, 09/01/2018 - 00:00
Dry-cured meat products are frequently colonised by toxigenic Penicillium nordicum and Penicillium verrucosum throughout their ripening that can produce ochratoxin A (OTA). The predictive nature of the molecular analyses can be used to determine the risk of toxigenic species. For this reason, the objective of the present work was to analyse the temporal changes in the...
Sat, 09/01/2018 - 00:00
A method was developed for the analysis of four polycyclic aromatic hydrocarbons regulated in the European Union, namely, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, and benzo[a]pyrene in edible oils. Multi-walled carbon nanotubes were applied for dispersive solid-phase extraction of analytes from the oils after dilution with n-hexane followed by elution of analytes...
Sat, 09/01/2018 - 00:00
In this work, surface-enhanced Raman spectroscopy (SERS) was used with silver colloid substrate for rapid detection of omethoate (an organophosphate pesticide) in standard solution and peach extract. The findings demonstrated that the characteristic wavenumber of the pesticide could be precisely identified using the SERS method. The calibration curve was plotted between...
Sat, 09/01/2018 - 00:00
A simple analytical method combining solvent extraction and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) was developed for the analysis of pesticide residues in rice bran protein powder. Owning to the high accuracy of HRMS in determination of mass to charge ratio (m/z), a suspect screen of pesticide residues was performed by LC-HRMS prior to...
Sat, 09/01/2018 - 00:00
A simple and rapid method of fluorescence polarization immunoassay (FPIA) for detection of Alternaria mycotoxin tenuazonic acid (TeA) was described. In this study, the tracers TeAH-FITC and TeAH-DTAF were synthesized from hapten 5-(sec-butyl)-3-(1-hydrazonoethyl)-4-hydroxy-1H-pyrrol-2(5H)-one (TeAH) and different fluoresceins isothiocyanate isomer I (FITC) or 5-([4,6-...
Sat, 09/01/2018 - 00:00
Tyramine signal amplification (TSA), the excellent signal amplification strategy, has the potential to improve the sensitivities of analytes analysis. In this work, the sensitivity of enzyme-linked immunosorbent assays (ELISA) has significantly improved by coupling TSA system and further biotin-streptavidin (BSA) system. Thus, a sensitive TSA-ELISA based on TSA was developed...
Wed, 08/01/2018 - 00:00
Food contamination caused by Staphylococcus aureus is a major problem worldwide, and more accurate and sensitive methods suitable for field detection are vital for S. aureus surveillance and control. Traditional methods for microbiological detection and identification are time-consuming and laborious. Although polymerase chain reaction-based methods are effective, the need...
Wed, 08/01/2018 - 00:00
The food-borne diseases triggered by Escherichia coli O157: H7 (E. coli O157: H7) have become a life-threatening problem for tens of thousands of people around the world in the past few decades. Therefore, a rapid, simple, and user-friendly biosensing label to monitor E. coli O157: H7 has become necessary and important. In this work, carboxyl functionalized graphene quantum...
Wed, 08/01/2018 - 00:00
In this paper, we proposed artificial fish swarm algorithm (AF) for synchronous selection of wavelengths and pretreatment methods during quantification of beef adulteration with spoiled beef based on the analysis of ultraviolet (UV), visible and near-infrared (Vis-NIR), and UV-Vis-NIR spectra. The best partial least squares regression model was obtained when the wavelength-...
Wed, 08/01/2018 - 00:00
An analytical method was developed and validated for the simultaneous determination in fish muscle of two kinds of emerging pollutants: polycyclic aromatic musks (galaxolide and tonalide) and UV filters [oxybenzone, 3-(4-methylbenzylidene) camphor, padimate-O, 2-ethylhexyl 4-methoxycinnamate, octocrylene], which are very common ingredients of personal care products, by...
Wed, 08/01/2018 - 00:00
Kanamycin (Kan) and tobramycin (Tob) are widely found in many foods of animal origin, including milk. More rapid, simple, and sensitive methods are urgently needed to monitor antibiotic residues in milk. An enhanced direct competitive enzyme-linked immunosorbent assay (dcELISA) based on gold nanoparticles (AuNPs)/horse radish peroxidase-Kan (HRP-Kan) was developed. A...
Fri, 06/01/2018 - 00:00
A multi-pesticide metabolite screening method using liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-Tof-MS) was developed for the detection of pesticide metabolites in fruit and vegetable samples. Based on a retrospectively created accurate mass compound database, a suspect screening approach was established for pesticides of high concern applied to...
Fri, 06/01/2018 - 00:00
The present study proposes an alternative analytical method for the determination of acrylamide in potato chips, using 2-naphthalenethiol as derivatization reagent followed by a HPLC separation with fluorescence detection to be used as a routine analytical procedure in the potato chip industry. The proposed method requires a relatively low-cost instrumentation compared to the...
Fri, 06/01/2018 - 00:00
Microcystins (MCs) pose a major threat to shellfish supplies, and humans that have been in contact with or consume contaminated products. To decrease health risks to the public, an efficient and accurate method for the synchronously determination of seven harmful MCs in shellfish samples was developed by employing a hydrophile lipophile balance/polydimethylsiloxane (HLB/PDMS...
Fri, 06/01/2018 - 00:00
Consumer and environmental groups have increasing concern about heavy metal contaminants and residual catalysts in plastics inducing negative human and environmental impacts. Regulations of heavy metals in food-contact plastics seek to reduce these risks by establishing maximum allowable threshold levels. However, traditional quantification methods of heavy metal...
Fri, 06/01/2018 - 00:00
A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation was developed for the simultaneous determination of 12 perfluorocarboxylic acids (PFCAs) and 7 perfluorosulfonic acids (PFSAs) in the muscle of shrimp, shellfish, and fish. A modified QuEChERS procedure was applied to the...
Fri, 06/01/2018 - 00:00
Glyphosate is the most widely used herbicide at the moment. It presents a broad spectrum of action, hence its use for many different crops. Regulatory agencies have constantly mentioned the low hazard potential to mammals. However, the International Agency for Research on Cancer concluded in 2015 that glyphosate is “probably carcinogenic to humans.” For this reason, it is...
Wed, 05/02/2018 - 00:00
Staphylococcus aureus (S. aureus) is of great importance and is a leading cause of food poisoning, which is a public health concern in terms of the frequency and seriousness of the disease. In the present study, RPA and real-time RPA assays were developed and validated to detect S. aureus with high sensitivity and specificity by targeting the nuc gene for the first time. The...
Tue, 05/01/2018 - 00:00
An analytical method based on liquid-liquid extraction combined with solid-phase extraction and isotope dilution-ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was well developed for simultaneous determination of aflatoxin B1 (AFB1), bisphenol A (BPA), and 4-nonylphenol (4-NP) in peanut oil. After adding isotope internal standards, the...
Tue, 05/01/2018 - 00:00
A group of methods were developed for the determination of etofenprox, kresoxim-methyl, cyprodinil, azoxystrobin, and pyrimethanil residues in animal-derived food (milk, egg, pig liver, pig fat, pork, and chicken) by using QuEChERS (quick, easy, cheap, effective, rugged, and safe)-gas chromatography/mass spectrometry (GC/MS). With acetonitrile as the extraction solvent, the...
Tue, 04/03/2018 - 00:00
Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption, thereby necessitating their effective monitoring and control. Antibody-based immunoassays provide efficient and cheap methods for screening this mycotoxin. To achieve high sensitivity and avoid enzymatic reaction, a...
Mon, 04/02/2018 - 00:00
The use of clenbuterol (CLE) as a growth-promoting agent in livestock is banned in the USA, European Union, and Mexico. Nevertheless, different studies have reported the presence of this agent in meat products, potentially causing intoxication in consumers. In this study, ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) is proposed for...
Sun, 04/01/2018 - 00:00
Determination of minerals is important for quality control of animal foods and sample preparation is a critical step to obtain fast, accurate, and reliable results. Extraction using dilute acid solutions is an interesting alternative due to its simplicity, softness, and safety. However, most extraction procedures are performed in batch mode, which generally is more time...
Thu, 03/01/2018 - 00:00
An accurate, rapid, and reliable multiresidue QuEChERS method based on gas chromatography coupled with tandem mass spectrometry was developed for the determination of 235 pesticides in challenging, dry, complex herb matrices (Centaurea cyanus L., Matricaria chamomilla L., Thymus vulgaris L.). Sample mass and the type of cleanup sorbent used to estimate the procedure’s...
Thu, 03/01/2018 - 00:00
A method of hollow fiber–stir bar sorptive extraction (HF–SBSE) coupled with gas chromatography–mass spectrometry was proposed to explore the migration of eight organochlorine pesticide residues in environmental and biological matrices. HF–SBSE was a configuration for solid–phase microextraction purposes, combining the features of SBSE and hollow fiber membrane extraction...

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