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Frontiers in Microbiology

Mon, 07/15/2019 - 20:00
Salmonella enterica is recognized as a major contributor of gastrointestinal illness worldwide. Concerns have been raised over the increasing prevalence of antibiotic resistant strains of Salmonella isolated from animals and food, and the role of antibiotics and other antimicrobial agents such as biocides and heavy metals in the selection and dissemination of antibiotic resistant bacteria to...
Mon, 07/15/2019 - 20:00
Vibrio parahaemolyticus causes the most seafood attributed gastroenteritis outbreaks worldwide and studies on pathogenesis during passage through the human digestive fluids are limited. An in vitro continuous model system mimicking passage through saliva, gastric and intestinal fluid was used to study the survival, morphology and virulence-related gene expression of 60 pathogenic and non-...
Wed, 07/10/2019 - 20:00
Gastrointestinal illnesses continue to be a global public health risk. Exposure to foodborne Salmonella directly or indirectly through consumption of ready-to-eat seafood can be an important route of infection to humans. This study was designed to estimate the population cell density, prevalence, virulence gene signatures and antibiotic resistance of Salmonella serovars from ready-to-eat shrimps...
Mon, 07/08/2019 - 20:00
The intimin protein is the major adhesin involved in the intimate adherence of atypical enteropathogenic Escherichia coli (aEPEC) strains to epithelial cells, but little is known about the structures involved in their early colonization process. A previous study demonstrated that the type III secretion system (T3SS) plays an additional role in the adherence of an Escherichia albertii strain....
Mon, 07/08/2019 - 20:00
Listeria monocytogenes the causative agent of listeriosis is an important public health concern and food safety challenge. Increased tolerance of this bacterium to benzalkonium chloride (BC), an antibacterial agent widely used in industrial settings, is a growing issue. Plasmid pLMST6 harbouring the gene of the multidrug efflux pump protein EmrC has been recently linked to enhanced BC tolerance...
Wed, 07/03/2019 - 20:00
With the increase in commercial pig farming, there is a simultaneous increase in the use of antibiotics for prophylaxis as well as therapeutics in China. In this study, we evaluated the prevalence and resistance diversity of Salmonella isolated from faeces of asymptomatic, live and slaughtered pigs. We analyzed 1,732 pig faecal samples collected over eight months, at Henan province of China....
Wed, 07/03/2019 - 20:00
Recent research has proven the ability of cold atmospheric plasma (CAP) for assuring food safety. A more flexible and transportable alternative is the use of plasma activated liquids (PAL), which are also known to have antimicrobial properties. However, within the context of food safety, little is known on its potential regarding decontamination. This research therefore focusses on identifying...
Tue, 07/02/2019 - 20:00
Research progress into mechanisms of the anaerobe Clostridium perfringens associated diseases has been frustrated by the lack of reliable infection models. Wax moth larvae (Galleria mellonella) have emerged as a viable alternative to other models of infection since they are economic, survive at 37°C and require no specialist equipment. This study aims to establish to what extent G. mellonella...
Tue, 07/02/2019 - 20:00
The aim of this study was to quantify the influence of temperature on pHmin-values of L. monocytogenes as used in cardinal parameter growth models and thereby improve the prediction of growth for this pathogen in food with low pH. Experimental data for L. monocytogenes growth in broth at different pH-values and at different constant temperatures were generated and used to determined pHmin-values...
Mon, 07/01/2019 - 20:00
Cronobacter sakazakii is an emerging foodborne pathogen, which is linked to life-threatening infections causing septicemia, meningitis and necrotizing enterocolitis. These infections have been epidemiologically connected to ingestion of contaminated reconstituted powder infant formula. Even at low water activity C. sakazakii can survive for a long time; it is capable of protective biofilm...
Tue, 06/25/2019 - 20:00
Not all Salmonella enterica serovars cause the same disease. S. enterica represents an incredibly diverse species comprising >2,600 unique serovars. While some S. enterica serovars are host-restricted, others infect a wide range of hosts. The diseases that nontyphoidal Salmonella (NTS) serovars cause vary considerably, with some serovars being significantly more likely to cause invasive...
Mon, 06/24/2019 - 20:00
Controlling aflatoxigenic Aspergillus flavus (Af) and aflatoxins in grains and food during storage are great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of Af in un-contacted face-to-face dual culture tests. Gas chromatography tandem mass spectrometry revealed that dimethyl...
Mon, 06/24/2019 - 20:00
Aflatoxin B1 (AfB1) is a carcinogenic mycotoxin that contaminates food and feed worldwide. We determined the AfB1-adsorption capability of non-viable Pleurotus eryngii mycelium, an edible fungus, as a potential means for removal of AfB1 from contaminated solutions. Lyophilized mycelium was produced and made enzymatically inert by sterilization at high temperatures . The material thus obtained...
Tue, 06/18/2019 - 20:00
Antimicrobial resistance was determined for 341 thermophilic Campylobacter jejuni isolates obtained from human clinical cases (n = 101), broiler products (n = 98), dairy cattle (n = 41) and wild birds (n = 101) with known multilocus sequence types (MLST) in Lithuania. The minimum inhibitory concentration (MIC) values for ciprofloxacin, tetracycline, gentamycin, ceftriaxone and erythromycin were...
Mon, 06/17/2019 - 20:00
Peroxyacetic acid (PAA) is widely applied in fresh apple packing facilities to reduce foodborne pathogens on fresh apples. However, there is limited data regarding its practical efficacy against Listeria monocytogenes on fresh apples. This study evaluated the antimicrobial activity of PAA against L. monocytogenes on fresh apples applicable to current industry practice, and further examined...
Mon, 06/17/2019 - 20:00
This study aims to investigate and compare the complete nucleotide sequences of the multidrug resistance plasmids pVb0267 and pVb0499, which were recovered from foodborne Vibrio parahaemolyticus isolates, and analyze the genetic environment of blaCTX-M-55 to provide insight into the dissemination mechanisms of this resistance element. Analysis of the sequences of plasmids pVb0267 (166,467 bp) and...
Tue, 06/11/2019 - 20:00
The aim of this study was to identify and characterize LA-MRSA in a very recent collection of staphylococci isolated from bovine quarter milk samples. All milk samples (n=14,924) sent to the MBFG in March 2017 were included into this study. The samples originated from 3,887 cows with 3,367 samples from 2,280 animals being positive for bacteria, prototheca and/or yeast. The second most common...
Tue, 06/04/2019 - 20:00
The emergence and increase in prevalence of resistance to cephalosporins amongst isolates of Salmonella from food animals imposes a public health threat. The aim of the present study was to investigate the prevalence and characteristics of CTX-M-producing Salmonella isolates from raw meat and food animals. 27 of 152 (17.76%) Salmonella isolates were ESBL-positive including 21/70 (30%) from food...
Thu, 05/30/2019 - 20:00
Studies have reported the occurrence of V. cholerae in fish but little is known about the interaction between fish and toxigenic V. cholerae as opposed to phytoplankton, which are well-established aquatic reservoirs for V. cholerae. The present study determined the role of tilapia (Oreochromis niloticus) as a reservoir host for survival and transmission of V. cholerae in aquatic environments....
Thu, 05/30/2019 - 20:00
Optical biosensors for rapid detection of significant foodborne pathogens are steadily gaining popularity due to its simplicity and sensitivity. While nanomaterials such as gold nanoparticles (AuNPs) are commonly used as signal amplifiers for optical biosensors, AuNPs can also be utilized as a robust biosensing platform. Many reported optical biosensors were designed for individual pathogen...
Tue, 05/28/2019 - 20:00
Yersinia enterocolitica is a major agent of foodborne diseases worldwide. Prophage plays an important role in the genetic evolution of the bacterial genome. Little is known about the genetic information about prophages in the genome of Y. enterocolitica, and no pathogenic Y. enterocolitica prophages have been described. In this study, we induced and described the genomes of six prophages from...
Mon, 05/27/2019 - 20:00
Campylobacter jejuni is one of the most common causes of human foodborne bacterial infections worldwide. The objective of this study was to assess the molecular diversity, using flaA sequencing, of 602 C. jejuni isolated from chicken food chain, i.e. chicken feces (n = 151), chicken carcasses (n = 150), chicken meat (n = 150) and from humans (n = 151) and to determine antimicrobial multiresistant...
Thu, 05/23/2019 - 20:00
Growth models are predominately used in the food industry to estimate the potential growth of select microorganisms under environmental conditions. The growth kinetics, cellular morphology and antibiotic resistance were studied throughout the life cycle of Salmonella Typhimurium. The effect of the previous life cycle phase (late log phase [LLP], early stationary phase [ESP], late stationary phase...
Thu, 05/23/2019 - 20:00
A multi drug resistant Salmonella enterica 4,[5],12:i- of sequence type 34 (monophasic S. Typhimurium ST34) is a current pandemic clone associated with livestock, particularly pigs, and numerous outbreaks in the human population. A large genomic island, termed SGI-4, is present in the monophasic Typhimurium ST34 clade and absent from other S. Typhimurium strains. SGI-4 consists of 87 open reading...
Mon, 05/20/2019 - 20:00
Whole-genome sequencing (WGS) will soon replace traditional phenotypic methods for routine testing of foodborne antimicrobial resistance (AMR). WGS is expected to improve AMR surveillance by providing a greater understanding of the transmission of resistant bacteria and AMR genes throughout the food chain, and therefore support risk assessment activities. At this stage, it is unclear how WGS data...
Thu, 05/16/2019 - 20:00
A total of forty-four lactic acid bacteria (LAB) strains originally isolated from cattle feces and different food sources were screened for their potential probiotic features. The antimicrobial activity of all isolates was tested by well-diffusion assay and competitive exclusion on broth against Salmonella Montevideo, Escherichia coli O157:H7 and Listeria monocytogenes strain N1-002. Thirty-eight...
Wed, 05/15/2019 - 20:00
Bacterial pathogens maintain disulfide bonds for protein stability and functions that are required for pathogenesis. Vibrio parahaemolyticus is a Gram-negative pathogen that causes food-borne gastroenteritis and is also an important opportunistic pathogen of aquatic animals. Two genes encoding the disulfide bond formation protein A, DsbA, are predicted to be encoded in the V. parahaemolyticus...
Mon, 05/13/2019 - 20:00
The aim of this study is to investigate the antimicrobial synergistic effect against Campylobacter jejuni, a leading foodborne pathogen that causes human gastroenteritis, by cinnamon oil, encapsulated curcumin, and zinc oxide nanoparticles (ZnO NPs). We compared three approaches to study the antimicrobial interactions, including the time-killing method, the fractional inhibitory concentration...
Wed, 05/08/2019 - 20:00
As WGS is increasingly used by food industry to characterize pathogen isolates, users are challenged by the variety of analysis approaches available, ranging from methods that require extensive bioinformatics expertise to commercial software packages. This study aimed to assess the impact of analysis pipelines (i.e., different hqSNP pipelines, a cg/wgMLST pipeline) and the reference genome...
Tue, 05/07/2019 - 20:00
Vibrio parahaemolyticus is a common pathogen which has become a major concern of seafood products. The bacteria in the viable but nonculturable (VBNC) state are unable to form colonies on growth media, but under appropriate conditions they can regain culturability. In this study, V. parahaemolyticus was induced into VBNC state at low temperature and oligotrophic condition, and was resuscitated to...
Tue, 05/07/2019 - 20:00
Listeria monocytogenes is an important foodborne pathogen with a significant impact on public health worldwide. A great number ofMany outbreaks caused by L. monocytogenes hasve been reported, especially in the United States and European countries. However, listeriosis has isuncommonnot yet been included ina notifiable disease in China, and thus information on this infection has been scarce among...
Mon, 05/06/2019 - 20:00
Salmonella is a major foodborne pathogen which causes widespread contamination and infection worldwide. Salmonella Typhimurium is one of the leading serovars responsible for human and animal salmonellosis, globally. The increasing rate of antibiotic resistance in Salmonella Typhimurium poses a significant global concern, and an improved understanding of the distribution of antibiotic resistance...
Mon, 05/06/2019 - 20:00
Listeria monocytogenes is a globally notoriousfoodborne pathogen. This study aimed to qualitatively and quantitatively detect L. monocytogenes from meat and meat products in China and to establish their virulence profiles and population diversity. From 1212 meat and meat product samples, 362 (29.9%) were positive for L. monocytogenes. Serogroup analysis showed that the most prevalent serogroup of...
Mon, 05/06/2019 - 20:00
Salmonella enterica serovar 4,[5],12:i:- (S. 4,[5],12:i:-) is an emerging foodborne pathogen causing salmonellosis in humans and animals. Probiotic Lactobacillus rhamnosus GG (LGG) is an effective strategy for controlling enteric infections through maintaining gut microbiota homeostasis and regulating the intestinal innate immune response. Here, LGG was orally administrated to newly weaned...
Tue, 04/30/2019 - 20:00
Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus Maofeng Zhang1,2†, Xiyan Zhang1†, Liaowang Tong1†, Dexin Ou1, Yaping Wang1, Jumei Zhang2, Qingping Wu2* and Yingwang Ye1,2* 1School of Food Science and Engineering, Hefei University of Technology, Hefei, China 2State Key Laboratory...
Mon, 04/29/2019 - 20:00
Surveillance and Genomics of Toxigenic Vibrio cholerae O1 From Fish, Phytoplankton and Water in Lake Victoria, Tanzania Yaovi M. Gildas Hounmanou1*, Pimlapas Leekitcharoenphon2, Rene S. Hendriksen2, Tamegnon V. Dougnon3, Robinson H. Mdegela4, John E. Olsen1 and Anders Dalsgaard1 1Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen...
Thu, 04/25/2019 - 20:00
Role of Starter Cultures on the Safety of Fermented Meat Products Marta Laranjo1*, Maria Eduarda Potes1,2 and Miguel Elias1,3 1ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Pólo da Mitra, Évora, Portugal 2Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Universidade de Évora, P...
Tue, 04/23/2019 - 20:00
Impact of ROS-Induced Damage of TCA Cycle Enzymes on Metabolism and Virulence of Salmonella enterica serovar Typhimurium Janina Noster1†, Marcus Persicke2, Tzu-Chiao Chao3, Lena Krone1, Bianca Heppner1, Michael Hensel1* and Nicole Hansmeier1,3,4* 1Abteilung Mikrobiologie, Universität Osnabrück, Osnabrück, Germany 2Microbial Genomics and Biotechnology, Center for...
Wed, 04/17/2019 - 20:00
Antimicrobial Resistance, Virulence Determinants, and Biofilm Formation of Enterococcus Species From Ready-to-Eat Seafood Etinosa O. Igbinosa1,2* and Abeni Beshiru1 1Applied Microbial Processes and Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria 2Sustainable Development Office, University of Benin, Benin...
Wed, 04/17/2019 - 20:00
Salmonella enterica Growth Conditions Influence Lettuce Leaf Internalization Yulia Kroupitski1, Rachel Gollop1, Eduard Belausov2, Riky Pinto1 and Shlomo Sela (Saldinger)1* 1Microbial Food-Safety Research Unit, Department of Food Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel 2Confocal Microscopy Unit, Institute of Plant Sciences, Agricultural...
Tue, 04/16/2019 - 20:00
Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae Susanne Brenzinger1*, Lizah T. van der Aart1, Gilles P. van Wezel1, Jean-Marie Lacroix2, Timo Glatter3 and Ariane Briegel1* 1Department of Microbial Biotechnology & Health, Institute of Biology Leiden, Leiden University, Leiden, Netherlands 2Unité de Glycobiologie Structurale et Fonctionnelle, UMR...
Tue, 04/16/2019 - 20:00
Large-Scale Genomic Analyses and Toxinotyping of Clostridium perfringens Implicated in Foodborne Outbreaks in France Abakabir Mahamat Abdelrahim, Nicolas Radomski, Sabine Delannoy, Sofia Djellal, Marylène Le Négrate, Katia Hadjab, Patrick Fach, Jacques-Antoine Hennekinne, Michel-Yves Mistou and Olivier Firmesse* Université PARIS-EST, Agence Nationale de Sécurité...
Thu, 04/11/2019 - 20:00
Environmental and Clinical Strains of Vibrio cholerae Non-O1, Non-O139 From Germany Possess Similar Virulence Gene Profiles Keike Schwartz, Jens Andre Hammerl, Cornelia Göllner and Eckhard Strauch* Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany Vibrio cholerae is a natural inhabitant of aquatic ecosystems globally. Strains of the...
Thu, 04/11/2019 - 20:00
Antimicrobial Resistance Diversity Suggestive of Distinct Salmonella Typhimurium Sources or Selective Pressures in Food-Production Animals Kate C. Mellor1,2*, Liljana Petrovska3, Nicholas R. Thomson2,4, Kate Harris3, Stuart W. J. Reid1 and Alison E. Mather5*† 1Royal Veterinary College, Hatfield, United Kingdom 2London School of Hygiene & Tropical Medicine, London, United...
Wed, 04/10/2019 - 20:00
Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Nicholas M. Negretti1, Geremy Clair2, Prabhat K. Talukdar1, Christopher R. Gourley1, Steven Huynh3, Joshua N. Adkins2, Craig T. Parker3, Colby M. Corneau1 and Michael E. Konkel1* 1School of Molecular Biosciences, College of Veterinary Medicine...
Tue, 04/09/2019 - 20:00
Identification of a nth-Like Gene Encoding an Endonuclease III in Campylobacter jejuni Lei Dai1, Jing Xia1,2, Orhan Sahin3 and Qijing Zhang1* 1Departments of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States 2National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai...

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