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Journal of Applied Microbiology

Wed, 07/10/2019 - 06:59
Abstract Aims The purposes were to evaluate the detection of low levels of Campylobacter in water by dead‐end ultrafiltration to determine the sensitivity and suitability for use under field condition. Methods And Results The dead‐end ultrafiltration technique followed by detection according to ISO 10272 was tested on artificially and naturally contaminated water. Campylobacter were detected...
Tue, 07/09/2019 - 08:58
Abstract Aims To develop a protocol for DNA extraction using whole milk and further, to investigate how the use of whole instead of skimmed milk during DNA extraction affected the resulting microbial composition. Methods and Results In a model study, three selected bacterial species (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 11775 and Lactobacillus reuteri PTA 4659) were added to...
Wed, 07/03/2019 - 07:13
Abstract Aims This investigation was undertaken to study the prevalence, enterotoxin gene profile and molecular epidemiology of Aeromonads from various sources of water (182) and fish (173). Methods and Results A total of 116 Aeromonas sp. were isolated, of which 48 (26·37%) were from water and 68 (34·62%) were from fish samples collected from retail markets and fish farms. The Aeromonads were...
Tue, 06/18/2019 - 04:08
Abstract Aims Manila clam (Ruditapes philippinarum) is one of the most popular seafood in Korea, owing to their unique taste and nutritional value. This study aimed to disclose the antibiotic and heavy‐metal resistance characteristics of Aeromonas spp. isolated from marketed Manila clam in Korea. Methods and results A total of 36 Aeromonas spp. strains were isolated and subjected to two tests...
Tue, 06/18/2019 - 04:07
Abstract Aim Study is focused on the influence of cadmium addition to growth media on production yield, their size and molecular mass of exopolysaccharides (EPS) synthesized by three rhizosphere bacteria strains. Inhibition of bacterial growth by increasing concentrations of Cd2+ was also analyzed. Methods and Results The highest impact of Cd2+ was noticed on growth of Arthrobacter sp. and R....
Mon, 06/17/2019 - 13:13
Abstract Aims The control of Listeria monocytogenes biofilm formation using lactocin AL705 bacteriocin at sub‐MICs through an anti‐quorum sensing strategy, was preliminarily investigated. Methods and Results The screening for biofilm formation of different Listeria species at 10 °C allowed selecting L. monocytogenes FBUNT for its use as biofilm producer. Minimum inhibitory concentration (MIC)...
Mon, 06/10/2019 - 15:04
Abstract Aim Shiga toxin‐producing Escherichia coli (STEC) cause bloody diarrhoea, kidney failure and occasionally death. However, identifying the source of infection caused by STEC other than serogroup O157 is hampered by availability of sensitive methods for detecting these pathogens. In this study we developed novel tools for detecting E. coli O55 that is potentially associated with human...
Fri, 06/07/2019 - 09:32
Abstract Aims To elucidate the antibiotic resistance and virulence genes of nisin‐resistant Enterococcus faecalis isolated from raw buffalo milk and to study the effect of nisin‐sensitive and ‐resistant E. faecalis on the innate immunity of rats. Methods and Results Slanetz–Bartley agar plates containing nisin were used to isolate nisin‐resistant E. faecalis. The virulence factors were...
Tue, 06/04/2019 - 15:14
Abstract Aims We developed a strategy for localized delivery of the LFCA (lactoferricin‐lactoferrampin), which is actively synthesized in situ by Lactococcus lactis (pAMJ399‐LFCA/LLMG1363), and explored the possibility of using the pAMJ399‐LFCA/LLMG1363 as an alternative additive diet to antibiotics. Methods and Results The antimicrobial activities of the LFCA derived from pAMJ399‐LFCA/...
Sat, 05/11/2019 - 10:14
Abstract Aim Intrauterine growth retardation (IUGR) is a prevalent problem in mammals. The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets. Methods and Results We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21, and 28 days of age using 16S rRNA sequencing. The results showed that IUGR piglets had a decreased...
Sat, 05/04/2019 - 15:44
Abstract Aims Greenhouse trials were conducted with different cultivars of baby leaf spinach, rocket, and Swiss chard and inoculation of Escherichia coli O157:H7 gfp+, to determine whether plant species and cultivar has an impact on establishment of this strain. Methods and results Three cultivars each of spinach, rocket and Swiss chard were spray inoculated with E. coli O157:H7 gfp+ at doses...
Tue, 04/30/2019 - 09:59
Abstract Use of bacteria in cancer therapy, despite being considered as a potent strategy, has not really picked up the way other methods of cancer therapies have evolved. However, in recent years, the interest on use of bacteria to kill cancer cells has renewed considerably. The standard and widely followed strategies of cancer treatment often fail either due to the complexity of tumor biology...
Mon, 04/29/2019 - 11:09
Abstract Aims In this study the association between multidrug resistance (MDR) and the expression of some virulence factors were evaluated in E. coli strains isolated from infant faeces and fresh green vegetables. The effect of isolate origin on associated virulence factors was evaluated. In addition, genetic fingerprinting of a sample of these isolates (10 isolates from each group) was studied...
Thu, 04/25/2019 - 06:40
Abstract Aims The aim of the study is to investigate the proteomic changes and antioxidant enzyme activity in chromium resistant Bacillus cereus VITSH1 in response to heavy metal toxicity. Methods and Results The variation in protein expression and antioxidant enzyme activity in the presence and absence of metal was studied in Bacillus cereus VITSH1. The differentially expressed proteins in...
Thu, 04/25/2019 - 06:40
Abstract Aims To evaluate ATP bioluminescence‐based hygiene monitoring systems under conditions relevant for fish processing environments. Methods and Results The ATP bioluminescence of fish fractions that are potentially present after insufficient cleaning of fish processing environments was determined. Different fractions and interfering substances representing the stages from slaughtering...
Fri, 04/19/2019 - 11:19
Abstract Aims The aim of this study was to develop a multiplex touchdown PCR (multiplex TD‐PCR) for rapid and simultaneous detection of four major foodborne pathogens to avoid mispriming and unwanted production during gene amplification. Touchdown PCR is the modified form of standard PCR, which enhances specificity, sensitivity. Methods and Results For this reason, a multiplex TD‐PCR assay...
Tue, 04/16/2019 - 06:51
Abstract Aims The aim of this study was the molecular identification of Salmonella enterica serovar Infantis isolated from poultry samples and their antimicrobial resistance and virulence profiling. Methods and Results A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected poultry post mortem specimens reported by us earlier were preliminary identified...
Mon, 04/15/2019 - 08:53
Abstract Aims This study aimed to gather information on farming practices employed in organic lettuce fields in Sao Paulo, Brazil and associate these practices with the microbiological characteristics of the products. Methods and Results Practices were surveyed using a questionnaire applied in ten farms, where 200 heads of lettuce were collected and submitted to enumeration of total coliforms...
Thu, 04/11/2019 - 05:56
Abstract Aim To determine the protective capacity against Salmonella infection in mice of the cell‐free fraction (postbiotic) of fermented milk, produced at laboratory and industrial level. Methods and Results The proteolytic activity (PA) of five commercial cultures and eleven autochthonous Lactobacillus strains was evaluated. The DSM‐100H culture displayed the highest PA and it was selected...
Fri, 03/29/2019 - 08:47
Abstract Aims Methicillin‐sensitive and methicillin‐resistant Staphylococcus aureus (MSSA and MRSA, respectively) in the nostrils of dogs and workers at an animal shelter were cultured. Staphylococcal toxin genes were analyzed to identify potential health concerns. Methods and Results Samples were obtained from 441 dogs and 9 workers. The respective isolation rates of S. aureus and MRSA were...
Wed, 03/27/2019 - 09:02
Abstract Aim The effect of heavy metals presence on the decolorization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated. Methods and results In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resistance to several heavy metals (Cu2+>Zn2+>Cd2+>Cr6+). Strain...
Tue, 03/26/2019 - 14:32
Abstract Aims The purpose of this research was to determine optimum conditions for broth recovery of heat‐injured Salmonella Typhimurium and Escherichia coli O157:H7. Methods and Results Exposure to 55°C for 15 and 25 min, respectively, induced cellular injury to those pathogens. Comparison was made with the commonly used overlay method using selective medium for recovering sub‐lethally...
Thu, 03/21/2019 - 14:51
Abstract Aims In this present study, the utility of a newly developed Loop mediated isothermal amplification (LAMP) and real‐time PCR assays designed to amplify the virB gene region of B. melitensis were evaluated from human clinical specimens. Methods and Results 54 culture confirmed cases of brucellosis and 54 culture negative but clinically suspected cases of brucellosis were included in...
Tue, 03/19/2019 - 09:47
Abstract Aims This study conducted bacterial community, virulence and antibiogram profiling inside the hindgut and skin of freshly caught hilsa fish and those sold at markets. Methods and Results The results of 16S rRNA‐based high‐throughput sequencing showed a higher number of bacterial genera in marketed fish samples than in fresh fish samples. The total operational taxonomic units (OTU),...
Thu, 03/07/2019 - 08:37
Abstract Sustainability and a more environment‐friendly approach is an emerging issue relevant to crop production. Abiotic stresses like drought, salinity, heat, cold or heavy metal pollution can severely compromise yields, and in this respect, plant protection practices should be highly efficient as well as safe for the environment and people. Among the many ways to achieve high productivity of...
Tue, 03/05/2019 - 15:18
Abstract Aims The aim of this study was to evaluate the genetic diversity and resistance phenotypes of Listeria monocytogenes strains isolated from clinical encephalitis cases, and compare this population to isolates derived from tank milk of healthy animals. Methods and results A total of 57 L monocytogenes strains isolated from ruminant's listeriosis cases (n=31) and from tank milk of...
Sun, 03/03/2019 - 13:43
Abstract Aims To investigate effects of the cationic surfactant cetyltrimethylammonium bromide (CTAB), a disinfectant, on spores of Bacillus species. Methods and results The ability of CTAB to trigger release of Bacillus spores’ large depot of dipicolinic acid (DPA) in a 1:1 chelate with Ca2+ (CaDPA), and to kill spores were investigated. CTAB triggered CaDPA release from spores of Bacillus...
Thu, 02/28/2019 - 10:22
Abstract Aims The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. Methods and Results The chemical composition of 16 EOs was determined using gas chromatography–mass spectrometry. All EOs were first...
Wed, 02/27/2019 - 08:43
Abstract Aims This study investigated the occurrence and genetic diversity of Enterobacteriaceae with extended‐spectrum β‐lactamase (ESBL)‐, AmpC‐ and carbapenemase‐mediated resistance in British beef cattle, and related risk factors. Methods and Results Faecal samples (n = 776) were obtained from farms in England and Wales (n = 20) and Scotland (n = 20) in 2015. Isolates from selective agars...
Wed, 02/20/2019 - 15:59
Abstract Aims Genotype VII Newcastle disease (ND) is one of the most epidemic and serious infectious diseases in the poultry industry. A novel vaccine targeting VII NDV is still in requirement. Methods and Results In this study, we constructed recombinant regulate delayed lysis Salmonella strains expressing either fusion protein (F) alone under eukaryotic CMV promoter or together with chicken...
Wed, 02/20/2019 - 14:11
Abstract Aims To evaluate the protective efficacy of Pediococcus pentosaceus GS4 in cadmium (Cd)‐induced toxicity in Swiss albino mice. Methods and Results The previously reported probiotic P. pentosaceus GS4 interaction with Cd was evaluated experimentally. Swiss albino mice were segregated randomly into three different groups containing nine animals each. The binding ability of P....
Mon, 02/18/2019 - 14:24
Abstract Aims The aim of this study was to evaluate the elimination of patulin (PAT) by Lactobacillus plantarum ATCC 8014 from artificially contaminated apple juice and its dependence on prebiotic, citric acid and ascorbic acid content. Methods and Results A central composite design was used for studying each of the three factors at five levels to find the optimum concentrations. The results...
Thu, 02/14/2019 - 15:04
Abstract Aim To investigate the use of a light scattering sensor, BARDOT (BActerial Rapid Detection using Optical scattering Technology) coupled with a multi‐pathogen selective medium, SEL (Salmonella, Escherichia, and Listeria) for concurrent detection of the three major foodborne pathogens in a single assay. Methods and Results BARDOT was used to detect and distinguish the three major...
Thu, 02/14/2019 - 15:00
Abstract Aim Recent concerns about the possible adverse effects of agricultural chemicals on health and environment have generated a considerable interest in biological alternatives. This study aimed to test the insecticidal potential of fungus Aspergillus flavus and revealed its genotoxic and cytotoxic effects using Spodoptera litura (Fabricius) as a model. Methods and Results The fungus was...
Thu, 02/14/2019 - 15:00
Abstract Aims The purpose of this study was to evaluate the effects of temperature, mixing and sunlight exposure on the 5‐day survival of Escherichia coli, Enterococcus sp., F+/male‐specific coliphages, somatic coliphages and Clostridium perfringens spores in an 80/20 blend of surface water and reclaimed water approved for potable reuse in North Carolina. Methods and Results Grab samples of...
Thu, 02/14/2019 - 15:00
Abstract Aims The current study aimed to investigate the ability of lactic acid bacteria (LABs) in removing four polycyclic aromatic hydrocarbons (PAHs) namely, benzo(a)pyrene (BaP), benz(a)anthracene (BaA), chrysene (Chr) and benzo(b)fluoranthene (BbF) from contaminated phosphate buffer saline (PBS). Method and Results The effect of initial PAH concentrations (5, 10, 15, 20 μg ml−1),...
Fri, 02/08/2019 - 10:14
Abstract Aims This study investigated the in vitro antibacterial activity of Hesperozygis ringens (Benth.) Epling leaf extracts against fish pathogenic bacteria, as well as the in vivo activity of the most active extract in silver catfish (Rhamdia quelen) experimentally infected with Aeromonas hydrophila. Moreover, the chemical composition of the extract used in the survival assay was evaluated...
Thu, 02/07/2019 - 07:49
Abstract Aims Field control of aflatoxin contamination is thought to occur through competitive exclusion of native aflatoxigenic fungi by introduced nonaflatoxigenic Aspergillus flavus biocontrol strains. In this study, we explored another possible mechanism that could increase the efficacy of biocontrol strains—the production of secreted compounds termed extrolites. Methods and Results Using...
Thu, 01/31/2019 - 13:03
Abstract Aims A small outbreak of gastroenteritis in 2011 in Apalachicola Bay, FL was attributed to consumption of raw oysters carrying Vibrio cholerae serotype O75. To better understand possible health risks, V. cholerae was surveyed in oysters, fish and seawater, and results were compared to data for Vibrio vulnificus and Vibrio parahaemolyticus. Methods and Results Enrichment protocols were...
Sun, 01/06/2019 - 14:34
Abstract Aims To obtain knowledge about 1) how to sample airborne MRSA and dust in the pig farm environment including effects of sampler on a) measured exposure, b) MRSA survival, and c) spatial and temporal variation in exposure, and 2) the association between exposure to MRSA, dust, and optical density. Methods and Results Airborne dust was sampled on five pig farms using five active and one...
Tue, 11/20/2018 - 11:49
Summary Chitosan has attracted a growing attention as a food preservative due to its versatility, nontoxicity, biodegradability and biocompatibility. This review aims to provide a critical appraisal of the limitations and opportunities of the use of chitosan as a food preservative. The application of chitosan as a food preservative necessitates insights into mechanisms of chitosan‐mediated cell...
Mon, 10/29/2018 - 15:00
Summary Viral enteropathogens are one of the leading causative agents of foodborne illnesses in both the United States and the European Union. While human noroviruses and hepatitis A virus cause the vast majority of outbreaks and illnesses, there are handful of human enteric viruses that contribute to sporadic outbreaks worldwide including astrovirus, sapovirus, rotavirus, enterovirus and Aichi...
Thu, 05/31/2018 - 15:08
Abstract Aim To develop a rapid, sensitive and specific diagnostic assay for detection of Brucella. Methods and result The probe based RT‐LAMP was carried out by using a set of four or six primers and different LAMP chemicals to compare its results with real time PCR. Detection of gene amplification is done within 40 minutes and can be seen by amplification curve, turbidity and addition of DNA...
Thu, 03/08/2018 - 09:12
Abstract AimThis study aimed to test biofilm inhibition activities of each of essential oils (EOs), main compounds of EOs and enzymes against pathogenic Klebsiella pneumoniae Methods and ResultsEffect of seven EOs and three enzymes was tested on formation and eradication of Klebsiella pneumoniae biofilm. Peppermint oil showed robust biofilm inhibitory effect, causing inhibition ranged from 69.2-...
Wed, 03/07/2018 - 06:06
Abstract AimsThe present study was designed to assess the phenotypic traits and virulence determinants of vegetables/fruits-origin E. coli and Salmonella strains. Methods and ResultsA total of 520 fresh vegetables/fruits samples were analyzed for the presence of E. coli, including Shiga-toxin producing E. coli (STEC), and Salmonella. The vegetables/fruits-origin E. coli and Salmonella strains...
Thu, 03/01/2018 - 13:55
Abstract AimsTo investigate the transmission route of Carnobacterium from the farm environment to the meat-manufacturing plant and potential risk for meat spoilage. Methods and ResultsA sheep farm level survey of Carnobacterium, consisting of 150 environmental and animal (no 100) associated samples, was carried out on two farms. A further 20 lamb carcass samples were taken from an abattoir...
Tue, 02/27/2018 - 07:25
Abstract AimsΑ systematic review to investigate fresh produce-borne viral outbreaks, tο record the outbreak distribution worldwide and to analyze the implication of different types of fresh produce and viral types as well. Methods and ResultsFour databases (PubMed/Medline, Scopus, Eurosurveillance Journal and Spingerlink electronic journal) and a global electronic reporting system (ProMED) were...
Fri, 02/23/2018 - 08:55
Abstract AimsThe effect of nutritional supplementation of two Metarhizium species with riboflavin (Rb) during production of conidia was (a) evaluated on conidial tolerance (based on germination) to UV-B radiation and (b) on conidial expression following UV-B irradiation, of enzymes known to be active in photoreactivation, viz., photolyase (Phr), laccase (Lcc) and polyketide synthase (Pks)....
Sat, 02/17/2018 - 08:20
Abstract AimsAeromonas hydrophila (A. hydrophila) has been isolated from various fish species in Egypt and is known to carry virulence and antimicrobial resistance genes, which pose risk for public health. The aim of the present study is to report, for the first time, the infection of mullet (Mugil cephalus) with A. hydrophila and to clarify the potential association between antimicrobial...
Sat, 02/10/2018 - 05:55
Abstract AimsTo develop a microassay method to detect antimicrobials produced by spore-forming bacteria, thus speeding up the discovery of new antimicrobials. Methods and resultsEnvironmental isolates were grown in 96-well plates, to allow production of antimicrobials agents, then treated with lysozyme and heat sequentially. Lysozyme-heat treatment inhibited or prevented spore-to-cell...

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