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Journal of Applied Microbiology

Thu, 03/08/2018 - 09:12
Abstract AimThis study aimed to test biofilm inhibition activities of each of essential oils (EOs), main compounds of EOs and enzymes against pathogenic Klebsiella pneumoniae Methods and ResultsEffect of seven EOs and three enzymes was tested on formation and eradication of Klebsiella pneumoniae biofilm. Peppermint oil showed robust biofilm inhibitory effect, causing inhibition ranged from 69.2-...
Wed, 03/07/2018 - 06:06
Abstract AimsThe present study was designed to assess the phenotypic traits and virulence determinants of vegetables/fruits-origin E. coli and Salmonella strains. Methods and ResultsA total of 520 fresh vegetables/fruits samples were analyzed for the presence of E. coli, including Shiga-toxin producing E. coli (STEC), and Salmonella. The vegetables/fruits-origin E. coli and Salmonella strains...
Thu, 03/01/2018 - 13:55
Abstract AimsTo investigate the transmission route of Carnobacterium from the farm environment to the meat-manufacturing plant and potential risk for meat spoilage. Methods and ResultsA sheep farm level survey of Carnobacterium, consisting of 150 environmental and animal (no 100) associated samples, was carried out on two farms. A further 20 lamb carcass samples were taken from an abattoir...
Tue, 02/27/2018 - 07:25
Abstract AimsΑ systematic review to investigate fresh produce-borne viral outbreaks, tο record the outbreak distribution worldwide and to analyze the implication of different types of fresh produce and viral types as well. Methods and ResultsFour databases (PubMed/Medline, Scopus, Eurosurveillance Journal and Spingerlink electronic journal) and a global electronic reporting system (ProMED) were...
Fri, 02/23/2018 - 08:55
Abstract AimsThe effect of nutritional supplementation of two Metarhizium species with riboflavin (Rb) during production of conidia was (a) evaluated on conidial tolerance (based on germination) to UV-B radiation and (b) on conidial expression following UV-B irradiation, of enzymes known to be active in photoreactivation, viz., photolyase (Phr), laccase (Lcc) and polyketide synthase (Pks)....
Sat, 02/17/2018 - 08:20
Abstract AimsAeromonas hydrophila (A. hydrophila) has been isolated from various fish species in Egypt and is known to carry virulence and antimicrobial resistance genes, which pose risk for public health. The aim of the present study is to report, for the first time, the infection of mullet (Mugil cephalus) with A. hydrophila and to clarify the potential association between antimicrobial...
Sat, 02/10/2018 - 05:55
Abstract AimsTo develop a microassay method to detect antimicrobials produced by spore-forming bacteria, thus speeding up the discovery of new antimicrobials. Methods and resultsEnvironmental isolates were grown in 96-well plates, to allow production of antimicrobials agents, then treated with lysozyme and heat sequentially. Lysozyme-heat treatment inhibited or prevented spore-to-cell...
Thu, 02/08/2018 - 08:40
Abstract AimThe study aimed to isolate and characterise Enterococcus species from apparently healthy waste attendants, cattle and cattle waste in Tanzania. Emphasis was given to antimicrobial resistance and in particular occurrence of vancomycin-resistant enterococci. Methods and ResultsFaecal samples were collected from healthy cattle, cattle waste attendants, and cattle house wastes and...
Tue, 02/06/2018 - 06:50
Abstract AimSurvival of Escherichia coli O157:H7 and Salmonella enterica in animal wastes-based composts was studied with different compost types, storage conditions, and inoculum levels. Methods and ResultsA cocktail of 3 E. coli O157:H7 or S. enterica strains was inoculated into dairy manure-based composts (A and B) or poultry litter-based composts (C and D), respectively, at final...
Tue, 02/06/2018 - 02:10
Abstract AimsGenomic characterization of Harveyi clade vibrio strain Y6 causing ‘Scale drop and Muscle Necrosis syndrome’ (SDMN) isolated from barramundi (Lates calcarifer) in Vietnam. Methods and ResultsA bacterial genome was sequenced using Illumina MiSeq platform. Multilocus sequence analysis confirmed that the bacterium belongs to Vibrio harveyi species. Further phylogenetic analysis inferred...
Fri, 02/02/2018 - 01:20
Abstract AimsThe aim of the study was to evaluate the microbicidal effectiveness of Radiant Catalytic Ionization (RCI) against L. monocytogenes strains in the form of planktonic cells and biofilm on food products and food contact surfaces as a method of food preservation. Methods and ResultsThe study material comprised six strains of L. monocytogenes, isolated from food. Samples of different...
Wed, 01/31/2018 - 23:55
Abstract AimThe study aim was to evaluate the potential of 405-nm light as a virus intervention for blueberries. Methods and ResultsTulane virus (TV)-inoculated blueberries were treated with 4·2 mW cm−2 of 405-nm light for 5–30 min. To mitigate thermal heating due to the intense light, a dry ice-chilled, nitrogen-based cooling system was utilized. Blueberries were rotated to ensure exposure of...
Sun, 01/21/2018 - 05:16
Abstract AimsThe study was focused on P. fluorescens strains isolated from Mozzarella cheese, with the aim of evaluating the effects of Origanum vulgare L. essential oil (OEO) on the biofilm formation and eradication, as well as on the motility and blue pigment production at 10°C. Methods and ResultsMicrodilution method was used to determine the minimum inhibitory and bactericidal concentration...
Fri, 01/19/2018 - 03:10
Abstract AimsThis study investigated the possible role of Fayoumi chickens and pigeons in the transmission of multidrug resistant (MDR) Salmonella with zoonotic potential. Methods and ResultsMorbid Fayoumi chickens (70) and pigeons (30) were examined to detect the prevalence and antibiotic resistance of Salmonella and to detect and sequence sodC-1 gene as a zoonotic and phylogenetic marker....
Thu, 01/18/2018 - 09:16
Abstract AimsThis research aims to assess total-cadmium soil content and microbiological aspects to understand the dynamics of culturable cadmium-tolerant bacteria (CdtB) in cacao soils from northeastern Colombia. Methods and ResultsAn integration of inverted dish plating, Cd determination and a microcalorimetry assay (IMC) was carried out. A farm in Boyacá showed the highest level of total soil...
Thu, 01/11/2018 - 01:23
Abstract AimsWe investigated whether Listeria monocytogenes strains differ in their ability to escape from the primary phagosome after internalization into human intestinal epithelial cells. Methods and ResultsFood and clinical strains were used to study specific alleles; the activities of listeriolysin O (LLO) and phospholipases PlcA and PlcB, which promote rupture of the phagocytic vacuole; and...
Sat, 01/06/2018 - 01:30
Abstract AimsTo examine Salmonella and Escherichia coli in storm runoff and irrigation ponds used by fresh produce growers, and compare Salmonella serovars with those found in cases of human salmonellosis. Methods and ResultsWe collected water before and after rain events at two irrigation ponds on farms in southern Georgia, USA, and collected storm runoff/storm flow within the contributing...
Wed, 12/27/2017 - 06:15
Abstract AimsDairy cattle shed pathogenic E. coli O157 (O157) in feces, playing a role in human exposure. We aimed to measure fecal microbial communities in early lactation dairy cattle, and model outcomes with O157 shedding metrics. Methods and ResultsDaily fecal samples were collected from 40 cattle on two Colorado dairies for five consecutive days, and characterized for O157. 16S rRNA gene...
Wed, 12/27/2017 - 06:06
Abstract AimsThe purpose of this survey was to estimate the respective prevalence of the “gang of seven” and “non-gang of seven” serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. Methods and ResultsAfter overnight enrichment growth the 69 intestinal contents testing...
Sat, 12/09/2017 - 12:20
Abstract AimThe spoilage potential of 28 bacterial strains isolated from spoiled raw yellowfin tuna was evaluated. Methods and ResultsBacterial species were inoculated in irradiated tuna matrix. Chemical changes, bacterial growth and sensory quality were monitored during aerobic storage at 8°C. Pseudomonas spp., Enterobacter spp. and Escherichia hermanii had no spoiling effect. Brochothrix...
Thu, 12/07/2017 - 09:20
Abstract AimsThis study aimed to review the microbiological results for raw drinking milk (RDM) samples submitted to Public Health England laboratories between 2014 and 2016 in order to produce up-to-date data on the microbiological safety of RDM and inform future risk assessments on its sale. Methods and ResultsA total of 902 samples of RDM were collected from retail sale in England for...
Thu, 12/07/2017 - 09:02
Abstract AimsTo compare the conventional plating method versus a fluorometric method using PrestoBlue® as a dye by determining the antimicrobial activity of two organic acids and curcumin against Salmonella Enteritidis in an avian in vitro digestion model that simulates the crop, proventriculus and intestine. Methods and ResultsA concentration of 108 cfu ml−1 of S. Enteritidis was exposed to...
Thu, 12/07/2017 - 08:54
Abstract AimsMolecular subtyping is commonly used in foodborne disease surveillance and microbial source tracking. There is a knowledge gap regarding the molecular ecology of foodborne pathogens in non-food associated environments. The objective of this study was to isolate and subtype foodborne pathogens from pristine natural environments with minimal anthropogenic inputs. Materials and...
Thu, 12/07/2017 - 08:45
Abstract AimsThe purpose of this study was to evaluate the effect of warehouse storage conditions on the composition of the fungal community of green coffee beans (GCB) that were stored in jute sacks for one year. Methods and ResultsMolecular characterization of the fungal community composition and population dynamics obtained by Q-PCR, CE-SSCP (Simpson's diversity index D), and Illumina MiSeq...
Tue, 11/28/2017 - 03:04
Abstract AimsThe incidence of community-associated Clostridium difficile infection (CA-CDI) in Australia has increased since mid-2011. With reports of clinically important C. difficile strains being isolated from retail foods in Europe and North America, a foodborne source of C. difficile in cases of CA-CDI is a possibility. This study represents the first to investigate the prevalence and...
Mon, 11/27/2017 - 09:35
Abstract AimsTo enhance the antimicrobial and antibiofilm activity of norfloxacin against the planktonic and biofilm mode of growth in ESKAPE pathogens using chemically modified norfloxacin salts. Methods and resultsAntimicrobial testing, synergy testing and time kill curve analysis, were performed to evaluate antibacterial effect of norfloxacin carboxylic acid salts against ESKAPE pathogens. In...
Wed, 11/22/2017 - 09:05
Abstract AimsTo select Listeria monocytogenes specific scFv antibodies from a phage display library by a novel simple and cost-effective immobilization method. Methods and ResultsLight Expanded Clay Aggregate (LECA) was used as biomass support matrix for biopanning of a phage display library to select Listeria monocytogenes specific scFv antibody. Four rounds of positive selection against LECA-...
Mon, 11/20/2017 - 04:55
Abstract AimsEnterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7 as a major human pathogen is responsible for food borne outbreaks, bloody diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS) and even death. In this study, the antibacterial activity of the Zataria multiflora essential oil (ZMEO) and nanoliposome-encapsulated ZMEO was evaluated on the pathogenicity of...
Thu, 11/09/2017 - 01:25
Abstract AimsPathogenic bacteria can spread between individuals or between food items via the surfaces they share. Limiting the survival of pathogens on surfaces therefore presents an opportunity to limit at least one route of how pathogens spread. In this study we propose that a simple coating with the essential oil isoeugenol can be used to circumvent the problem of bacterial transfer via...
Tue, 11/07/2017 - 10:01
Abstract AimsThe objective of this study was to show whether the edible part of broccoli has antibacterial and antifungal activity against microorganism of importance in human health and vegetable spoilage, and to test if this effect was partially due to antimicrobial peptides. Methods and ResultsCrude extracts were obtained from florets and stems of broccoli cultivar Avenger and the inhibitory...
Mon, 11/06/2017 - 15:25
Abstract AimsMicrobiological, biotechnological and chemical characterization of a previously undescribed aryl-crowned polyketide from Bacillus subtilis MTCC 10403 isolated from brown seaweed Anthophycus longifolius with activity against opportunistic Gram-negative food-borne pathogenic bacterial strains. Methods and ResultsA culture-dependent method was used to isolate heterotrophic B. subtilis...
Fri, 11/03/2017 - 00:45
Abstract AimsThe aim of the study was to examine the effect of different process parameters which including; initial juglone amount, initial poly(d,l-lactide co-glycolide) amount, polyvinyl alcohol volume and polyvinyl alcohol concentration on encapsulation of juglone to poly(d,l-lactide co-glycolide) nanoparticles. Methods and ResultsThe synthesized nanoparticle formulations were analyzed for...
Thu, 11/02/2017 - 03:45
Summary Galicia (NW Spain) has 1,490 km of coastline, and its particular topography, characterized by the presence of fiord-like inlets, called rías, with an important primary production, make this region very favourable for shellfish growth and culture. In fact, Galicia is one of the most important mussel producers in the world. Due to the proximity to cities and villages, and the anthropogenic...
Tue, 10/17/2017 - 02:36
Abstract AimsThe objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). Methods and ResultsA total of 243 isolates were evaluated in terms of antimicrobial resistance...
Tue, 10/17/2017 - 02:31
Abstract AimIn this study, the effects of addition of salt to treatment with acids (one of several organic acids and salt in various solutions including rich or minimal broth, buffer, or distilled water) on the reduction of Escherichia coli O157:H7 were investigated. The protein expression profiles corresponding to acid stress (acetic acid) with or without salt addition were studied using a...
Tue, 10/10/2017 - 06:46
Abstract AimsThe control of Salmonella in pig production is necessary for public and animal health and vaccination was evaluated as a strategy to decrease pig prevalence. Methods and ResultsThe study examined the efficacy of a live Salmonella Typhimurium vaccine, administered to sows on eight commercial farrow-to-finish herds experiencing clinical salmonellosis or Salmonella carriage associated...
Tue, 10/10/2017 - 06:40
Abstract AimsThis study aimed to investigate the efficacy of neutral electrolyzed water (NEW) on biofilm and planktonic forms of Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa in Luria-Bertani (LB) broth, 0.5 and 3% w/w fat pasteurized and UHT milk. ‘ Methods and ResultsBiofilm was established on stainless steels in a simulated industrial model biofilm reactor. NEW had a...
Tue, 09/26/2017 - 02:30
Abstract AimsTo determine the fate of E. coli on vegetables that were processed through commercial wash treatments and stored under simulated retail conditions at 4°C or wholesale at fluctuating ambient temperatures (0-25°C, dependent on season). Methods and ResultsBovine slurry that was naturally contaminated with Escherichia coli O145 was applied without dilution or diluted 1/10 using borehole...
Mon, 09/25/2017 - 04:31
Abstract AimsTo examine the influence of meat plant E. coli and Salmonella sp. isolates on E. coli O157 biofilm formation. Methods and ResultsBiofilm formation was quantified by crystal violet staining (A570 nm) and viable cell numbers for up to 6 days at 15°C. All five persistent E. coli genotypes formed strong biofilms when cultured alone or co-cultured with E. coli O157, with A570 nm values...
Mon, 09/04/2017 - 14:35
Abstract AimsTo analyse the function of a putative lantibiotic gene cluster of Paenibacillus polymyxa E681 and to characterize its product, paenilan. Methods and ResultsComparative analysis of the lantibiotic gene cluster of E681 revealed that the cluster, consisting of 11 open reading frames, is involved in the biosynthesis of a class I lantibiotic. The pnlA gene encoding the prepeptide PnlA was...
Sun, 08/20/2017 - 07:30
Abstract AimsTo assess diarrheal risks from enteropathogenic E. coli, Giardia, and Cryptosporidium from consuming raw spinach, cabbage, carrots, and tomatoes in Kathmandu Valley, Nepal. Methods and ResultsThe annual infection risk was quantified using the probabilistic Quantitative Microbial Risk Assessment approach, which considered 12 vegetable washing combinations. A new model was used to...
Fri, 08/18/2017 - 03:50
Abstract AimTo assess the potential of a viability dye and an enzymatic RT-qPCR pretreatment to discriminate between infectious and non-infectious enteric viruses. Methods and resultsEnterovirus, Norovirus GII.4 and Hepatitis A virus were inactivated at 95 °C for 10 min and four methods were used to compare the efficiency of inactivation: (a) cell culture plaque assay for Hepatitis A virus and...
Thu, 08/17/2017 - 04:41
Abstract AimThe activity of chitosan microparticles (CM) was examined using a matrix of conditions in order to assess the efficacy of CM as a mitigation against various strains of Salmonella enterica in agricultural water. Methods and ResultsDifferent concentrations of CM (0, 0.01, 0.1, 0.2, 0.3% w/v) were examined for antimicrobial activity against log vs. stationary phase cells of Salmonella...
Thu, 08/17/2017 - 04:36
Abstract AimsIdentify novel bacterial taxa that could increase the availability of branched-chain amino acids and the amount of distinctive volatiles during skim milk fermentation. Methods and ResultsWe recovered 344 bacterial isolates from stool samples of healthy and breastfed infants. Five were selected based on their ability to produce branched-chain amino acids. Three strains were identified...
Wed, 08/16/2017 - 00:07
Abstract AimsLI-Fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (Gram-positive bacteria and fungi). In this study, LI-F-type antimicrobial peptides (AMP-jsa9) composing of LI-F03a, LI-F03b, LI-F04a, LI-F04b and LI-F05b were isolated from Paenibacillus polymyxa JSA-9. To better understand the antimicrobial mechanism of AMP-jsa9, the potency...
Fri, 08/11/2017 - 04:20
Abstract AimsThe aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l−1), caused by cross-contamination of feed, on the enrichment of a DOX resistant commensal E. coli and it's resistance plasmid in an ex vivo model of the porcine cecum. Methods and ResultsA DOX resistant, tet(A) carrying, porcine commensal E. coli strain (EC...
Wed, 08/09/2017 - 20:00
Abstract AimsTo investigate the influence of irrigation water microbial quality on leafy green vegetables produced in commercial and small-scale farms as well as homestead gardens using pyrosequencing. Methods and resultsNext generation sequencing analysis of the V1-V3 hypervariable region of bacterial 16S rDNA was used to compare bacterial diversity in irrigation water sources and on leafy...
Wed, 07/26/2017 - 04:01
Abstract This review is an account of experiences of two research teams (from Italy and Spain); the leading idea is the following: yeasts represent valuable sources in food science and microbiology and are a kind of food factories, because of the potentiality of whole cells or for their produced compounds. The review covers three major areas: the first section addresses the role of yeasts as...
Mon, 07/24/2017 - 00:20
Abstract AimsThe objective of this study was to determine if the fecal microbiome has an association with E. coli O157:H7 prevalence and enumeration. Methods and ResultsPyrosequencing analysis of fecal microbiome was performed from feedlot cattle fed one of three diets: i) 94 heifers fed low concentrate (LC) diet, ii) 142 steers fed moderate concentrate (MC) diet, and iii) 132 steers fed high...
Fri, 07/14/2017 - 10:40
Abstract AimsTo investigate the antimicrobial properties of E. coccinea and E. sessilanthera and to isolate and identify the antimicrobial compounds. Methods and resultsExtracts were obtained via sequential solvent extraction method using hexane, dichloromethane, ethyl acetate, methanol and water. Antimicrobial activity testing was done using broth microdilution assay against 17 strains of...

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