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Journal of Applied Microbiology

Thu, 12/07/2017 - 08:45
Abstract AimsThe purpose of this study was to evaluate the effect of warehouse storage conditions on the composition of the fungal community of green coffee beans (GCB) that were stored in jute sacks for one year. Methods and ResultsMolecular characterization of the fungal community composition and population dynamics obtained by Q-PCR, CE-SSCP (Simpson's diversity index D), and Illumina MiSeq...
Tue, 11/28/2017 - 03:04
Abstract AimsThe incidence of community-associated Clostridium difficile infection (CA-CDI) in Australia has increased since mid-2011. With reports of clinically important C. difficile strains being isolated from retail foods in Europe and North America, a foodborne source of C. difficile in cases of CA-CDI is a possibility. This study represents the first to investigate the prevalence and...
Mon, 11/27/2017 - 09:35
Abstract AimsTo enhance the antimicrobial and antibiofilm activity of norfloxacin against the planktonic and biofilm mode of growth in ESKAPE pathogens using chemically modified norfloxacin salts. Methods and resultsAntimicrobial testing, synergy testing and time kill curve analysis, were performed to evaluate antibacterial effect of norfloxacin carboxylic acid salts against ESKAPE pathogens. In...
Wed, 11/22/2017 - 09:05
Abstract AimsTo select Listeria monocytogenes specific scFv antibodies from a phage display library by a novel simple and cost-effective immobilization method. Methods and ResultsLight Expanded Clay Aggregate (LECA) was used as biomass support matrix for biopanning of a phage display library to select Listeria monocytogenes specific scFv antibody. Four rounds of positive selection against LECA-...
Mon, 11/20/2017 - 04:55
Abstract AimsEnterohaemorrhagic Escherichia coli (EHEC) serotype O157:H7 as a major human pathogen is responsible for food borne outbreaks, bloody diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS) and even death. In this study, the antibacterial activity of the Zataria multiflora essential oil (ZMEO) and nanoliposome-encapsulated ZMEO was evaluated on the pathogenicity of...
Thu, 11/09/2017 - 01:25
Abstract AimsPathogenic bacteria can spread between individuals or between food items via the surfaces they share. Limiting the survival of pathogens on surfaces therefore presents an opportunity to limit at least one route of how pathogens spread. In this study we propose that a simple coating with the essential oil isoeugenol can be used to circumvent the problem of bacterial transfer via...
Tue, 11/07/2017 - 10:01
Abstract AimsThe objective of this study was to show whether the edible part of broccoli has antibacterial and antifungal activity against microorganism of importance in human health and vegetable spoilage, and to test if this effect was partially due to antimicrobial peptides. Methods and ResultsCrude extracts were obtained from florets and stems of broccoli cultivar Avenger and the inhibitory...
Mon, 11/06/2017 - 15:25
Abstract AimsMicrobiological, biotechnological and chemical characterization of a previously undescribed aryl-crowned polyketide from Bacillus subtilis MTCC 10403 isolated from brown seaweed Anthophycus longifolius with activity against opportunistic Gram-negative food-borne pathogenic bacterial strains. Methods and ResultsA culture-dependent method was used to isolate heterotrophic B. subtilis...
Fri, 11/03/2017 - 00:45
Abstract AimsThe aim of the study was to examine the effect of different process parameters which including; initial juglone amount, initial poly(d,l-lactide co-glycolide) amount, polyvinyl alcohol volume and polyvinyl alcohol concentration on encapsulation of juglone to poly(d,l-lactide co-glycolide) nanoparticles. Methods and ResultsThe synthesized nanoparticle formulations were analyzed for...
Thu, 11/02/2017 - 03:45
Summary Galicia (NW Spain) has 1,490 km of coastline, and its particular topography, characterized by the presence of fiord-like inlets, called rías, with an important primary production, make this region very favourable for shellfish growth and culture. In fact, Galicia is one of the most important mussel producers in the world. Due to the proximity to cities and villages, and the anthropogenic...
Tue, 10/17/2017 - 02:36
Abstract AimsThe objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). Methods and ResultsA total of 243 isolates were evaluated in terms of antimicrobial resistance...
Tue, 10/17/2017 - 02:31
Abstract AimIn this study, the effects of addition of salt to treatment with acids (one of several organic acids and salt in various solutions including rich or minimal broth, buffer, or distilled water) on the reduction of Escherichia coli O157:H7 were investigated. The protein expression profiles corresponding to acid stress (acetic acid) with or without salt addition were studied using a...
Tue, 10/10/2017 - 06:46
Abstract AimsThe control of Salmonella in pig production is necessary for public and animal health and vaccination was evaluated as a strategy to decrease pig prevalence. Methods and ResultsThe study examined the efficacy of a live Salmonella Typhimurium vaccine, administered to sows on eight commercial farrow-to-finish herds experiencing clinical salmonellosis or Salmonella carriage associated...
Tue, 10/10/2017 - 06:40
Abstract AimsThis study aimed to investigate the efficacy of neutral electrolyzed water (NEW) on biofilm and planktonic forms of Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa in Luria-Bertani (LB) broth, 0.5 and 3% w/w fat pasteurized and UHT milk. ‘ Methods and ResultsBiofilm was established on stainless steels in a simulated industrial model biofilm reactor. NEW had a...
Tue, 09/26/2017 - 02:30
Abstract AimsTo determine the fate of E. coli on vegetables that were processed through commercial wash treatments and stored under simulated retail conditions at 4°C or wholesale at fluctuating ambient temperatures (0-25°C, dependent on season). Methods and ResultsBovine slurry that was naturally contaminated with Escherichia coli O145 was applied without dilution or diluted 1/10 using borehole...
Mon, 09/25/2017 - 04:31
Abstract AimsTo examine the influence of meat plant E. coli and Salmonella sp. isolates on E. coli O157 biofilm formation. Methods and ResultsBiofilm formation was quantified by crystal violet staining (A570 nm) and viable cell numbers for up to 6 days at 15°C. All five persistent E. coli genotypes formed strong biofilms when cultured alone or co-cultured with E. coli O157, with A570 nm values...
Mon, 09/04/2017 - 14:35
Abstract AimsTo analyse the function of a putative lantibiotic gene cluster of Paenibacillus polymyxa E681 and to characterize its product, paenilan. Methods and ResultsComparative analysis of the lantibiotic gene cluster of E681 revealed that the cluster, consisting of 11 open reading frames, is involved in the biosynthesis of a class I lantibiotic. The pnlA gene encoding the prepeptide PnlA was...
Sun, 08/20/2017 - 07:30
Abstract AimsTo assess diarrheal risks from enteropathogenic E. coli, Giardia, and Cryptosporidium from consuming raw spinach, cabbage, carrots, and tomatoes in Kathmandu Valley, Nepal. Methods and ResultsThe annual infection risk was quantified using the probabilistic Quantitative Microbial Risk Assessment approach, which considered 12 vegetable washing combinations. A new model was used to...
Fri, 08/18/2017 - 03:50
Abstract AimTo assess the potential of a viability dye and an enzymatic RT-qPCR pretreatment to discriminate between infectious and non-infectious enteric viruses. Methods and resultsEnterovirus, Norovirus GII.4 and Hepatitis A virus were inactivated at 95 °C for 10 min and four methods were used to compare the efficiency of inactivation: (a) cell culture plaque assay for Hepatitis A virus and...
Thu, 08/17/2017 - 04:41
Abstract AimThe activity of chitosan microparticles (CM) was examined using a matrix of conditions in order to assess the efficacy of CM as a mitigation against various strains of Salmonella enterica in agricultural water. Methods and ResultsDifferent concentrations of CM (0, 0.01, 0.1, 0.2, 0.3% w/v) were examined for antimicrobial activity against log vs. stationary phase cells of Salmonella...
Thu, 08/17/2017 - 04:36
Abstract AimsIdentify novel bacterial taxa that could increase the availability of branched-chain amino acids and the amount of distinctive volatiles during skim milk fermentation. Methods and ResultsWe recovered 344 bacterial isolates from stool samples of healthy and breastfed infants. Five were selected based on their ability to produce branched-chain amino acids. Three strains were identified...
Wed, 08/16/2017 - 00:07
Abstract AimsLI-Fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (Gram-positive bacteria and fungi). In this study, LI-F-type antimicrobial peptides (AMP-jsa9) composing of LI-F03a, LI-F03b, LI-F04a, LI-F04b and LI-F05b were isolated from Paenibacillus polymyxa JSA-9. To better understand the antimicrobial mechanism of AMP-jsa9, the potency...
Fri, 08/11/2017 - 04:20
Abstract AimsThe aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l−1), caused by cross-contamination of feed, on the enrichment of a DOX resistant commensal E. coli and it's resistance plasmid in an ex vivo model of the porcine cecum. Methods and ResultsA DOX resistant, tet(A) carrying, porcine commensal E. coli strain (EC...
Wed, 08/09/2017 - 20:00
Abstract AimsTo investigate the influence of irrigation water microbial quality on leafy green vegetables produced in commercial and small-scale farms as well as homestead gardens using pyrosequencing. Methods and resultsNext generation sequencing analysis of the V1-V3 hypervariable region of bacterial 16S rDNA was used to compare bacterial diversity in irrigation water sources and on leafy...
Wed, 07/26/2017 - 04:01
Abstract This review is an account of experiences of two research teams (from Italy and Spain); the leading idea is the following: yeasts represent valuable sources in food science and microbiology and are a kind of food factories, because of the potentiality of whole cells or for their produced compounds. The review covers three major areas: the first section addresses the role of yeasts as...
Mon, 07/24/2017 - 00:20
Abstract AimsThe objective of this study was to determine if the fecal microbiome has an association with E. coli O157:H7 prevalence and enumeration. Methods and ResultsPyrosequencing analysis of fecal microbiome was performed from feedlot cattle fed one of three diets: i) 94 heifers fed low concentrate (LC) diet, ii) 142 steers fed moderate concentrate (MC) diet, and iii) 132 steers fed high...
Fri, 07/14/2017 - 10:40
Abstract AimsTo investigate the antimicrobial properties of E. coccinea and E. sessilanthera and to isolate and identify the antimicrobial compounds. Methods and resultsExtracts were obtained via sequential solvent extraction method using hexane, dichloromethane, ethyl acetate, methanol and water. Antimicrobial activity testing was done using broth microdilution assay against 17 strains of...
Fri, 07/14/2017 - 10:35
Abstract AimsThe aim of the present work is to report on the hydrolytic action of Alcaligenes faecalis isolated from soil samples and its ability to degrade ochratoxin A. Methods and ResultsAn Alcaligenes faecalis strain was identified and characterized by employing both a phenotypic analysis and 16S rDNA sequence analysis. The results show that this strain could degrade ochratoxin A efficiently...
Fri, 07/14/2017 - 10:35
Abstract AimsThe aim of the study was to explore irrigation water polluted with industrial waste and corresponding cropland to screen bacteria for As-detoxification and plant growth promotion. Methods and ResultsPlant growth promoting As-resistant cropland bacteria were isolated from contaminated irrigation water and corresponding agricultural soil. Phylogenetic analysis revealed that the...
Fri, 07/14/2017 - 10:31
Abstract AimInvestigate dynamics of culturable mesophilic bacteria and selected food contaminating bacteria from three herbs and their production environment. Methods and ResultsMarjoram, basil, and thyme were investigated during one growing season by sampling plants, organic fertilizers, soil, irrigation water, and marketed products. Mesophilic bacteria and selected food contaminating bacteria (...
Mon, 06/26/2017 - 10:02
Abstract AimsCharacterize the phosphate-specific transport system in Cronobacter sakazakii BAA-894. Methods and ResultsThe genes relevant to phosphate transfer in C. sakazakii BAA-894 were determined by using sequence alignment to the corresponding genes in Escherichia coli. Then, the determined pst operon in C. sakazakii BAA-894 was deleted using the lambda Red recombination system. Using the...
Mon, 06/26/2017 - 02:42
Abstract AimTo improve the efficacy of intercalating dyes to distinguishing between infectious and inactivated hepatitis A virus (HAV) in food. Methods and resultsDifferent intercalating dyes were evaluated for the discrimination between infectious and thermally-inactivated HAV suspensions combining with the RT-qPCR proposed in the ISO 15216. Among them, PMAxx was the best dye in removing the RT-...
Fri, 06/23/2017 - 12:20
Abstract AimsThis study aimed to evaluate the biofilm formation and disinfectant resistance of Salmonella cells in mono- and dual-species biofilms with Pseudomonas aeruginosa, and to investigate the role of extracellular polymeric substances (EPS) in protection of biofilms against disinfection treatment. Methods and ResultsThe populations of Salmonella in mono- or dual-species biofilms with P....
Wed, 06/21/2017 - 19:35
Abstract AimTo isolate and characterize indigenous gluten hydrolyzing bacteria from wheat sourdough and curd samples and further evaluation of their probiotic potentiality. Methods and ResultsIndigenous gluten hydrolyzing isolates GS 33, GS 143, GS 181 and GS 188 were identified as Bacillus spp. by molecular-typing methods and studied extensively for their functional and probiotic attributes. All...
Mon, 06/05/2017 - 09:30
Abstract AimsThis study aimed to evaluate the effect of five cadmium-tolerant endophytic plant growth-promoting bacteria (PGPB) strains on the biomass and cadmium tolerance and accumulation of the bioenergy plant switchgrass (Panicum virgatum L.) under cadmium (Cd)-contaminated conditions. Methods and ResultsFive bacterial strains (Bj05, Le14, Ps14, So02, and Bo03) that could tolerate up to 2000...
Mon, 05/29/2017 - 09:30
Abstract Aimsthe study investigated the use of bacteriophages to control bacterial contamination of chicken skin, eggs, tomatoes and meat. Methods and ResultsExperiments were performed to test the host ranges and killing potential of two isolated phages, ZCSE1 and ZCEC1 with hosts Salmonella and E. coli respectively. The efficacy of both phages was determined by comparing the viable counts of...
Wed, 05/03/2017 - 22:40
Abstract AimTo gain deeper insights into the clostridial community dynamics and chemical transformations during the ensiling of alfalfa. Methods and ResultsDirect-cut alfalfa silage (with the dry matter content of 240 g kg−1) was prepared with or without the addition of a lactic acid bacterial inoculant and sucrose. Silages were sampled at 0, 3, 7, 14, 28 and 56 days after ensiling and their...
Fri, 04/28/2017 - 04:05
Abstract AimsListeria monocytogenes nisin resistance increases when first exposed to NaCl and other stresses, such as low pH. In addition to environmental stressors, specific genomic elements can confer nisin resistance, such as the stress survival islet (SSI-1). As SSI-1 is variably present among L. monocytogenes strains, we wanted to determine if SSI-1 was associated with salt-induced nisin...
Tue, 04/04/2017 - 10:20
Abstract AimsTo elucidate the biological characteristics and stability of a newly identified staphylococcal enterotoxin Q (SEQ) against heating and digestive enzymes, and evaluate the risk of seq-harboring S. aureus in food poisoning. Methods and ResultsPurified SEQ was treated with heating, pepsin and trypsin that are related to food cooking, stomach and intestine conditions. Superantigenic...
Tue, 04/04/2017 - 00:25
Abstract AimsThe thermal resistance of desiccation-adapted Salmonella Senftenberg 775/W was compared with those of indigenous enterococci and total aerobic bacteria in poultry litter. Methods and ResultsAged broiler litter and composted turkey litter with 20, 30, 40, and 50% moisture contents were inoculated with desiccation-adapted S. Senftenberg 775/W, and then heat-treated at 75 and 85°C....
Sun, 04/02/2017 - 08:21
Abstract AimThe antibacterial activities of chitosan (CS) and its derivative chitosan oligosaccharide lactate (COL) were evaluated against Aeromonas hydrophila, Edwardsiella ictaluri and Flavobacterium columnare, three highly pathogenic bacteria of warmwater finfish. Methods and ResultsThe magnitude and mode of antimicrobial action on gram negative bacterial pathogens was investigated with an...
Wed, 03/29/2017 - 10:46
Abstract AimsThis study aimed to determine the effect of a cell-free supernatant of Lactobacillus paracasei ATCC27092 with and without rice bran extract (RBE) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. Methods and ResultsSupernatant was collected from overnight cultures of L. paracasei incubated in the presence (LP+RBE) or absence (...
Mon, 03/27/2017 - 02:55
Abstract AimA cross-sectional study was conducted in ten government organized pig farms between 2014 and 2016 representing seven states of India to understand the epidemiology of carbapenem resistance in Escherichia coli isolates. Methods and ResultsIn this study, 673 fecal samples both from non- diarhhoeic (n=501) and diarrhoeic (n=172) piglets were processed for isolation of carbapenem...
Thu, 03/16/2017 - 12:10
Abstract AimsQuantify aflatoxins, the potent carcinogens associated with stunting and immune suppression, in maize and groundnut across Zambia's three agroecologies and determine vulnerability to aflatoxin increases after purchase. Methods and ResultsAflatoxin concentrations were determined for 334 maize and groundnut samples from 27 districts using lateral-flow immunochromatography. Seventeen...
Mon, 03/06/2017 - 02:30
Abstract AimsThe spread of bacteria that are simultaneously resistant to disinfectants and antimicrobials would constitute an unsettling scenario. In order to explore an association between antimicrobial resistance and reduced susceptibility to biocides / microbicides like disinfectants in agriculture, we investigated Escherichia (E.) coli (n = 438) and enterococci (n = 120) isolated from six...
Sat, 03/04/2017 - 02:10
Abstract AimsTo identify production and processing practices that might reduce Campylobacter numbers contaminating chicken broiler carcasses. Methods and ResultsNumbers of campylobacters were determined on carcass neck skins after processing or in broiler house litter samples. Supplementary information that described farm layouts, farming conditions for individual flocks, the slaughterhouse...
Fri, 03/03/2017 - 01:55
Abstract AimsThis study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (Oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0.6% mannan-oligosaccharides and 0.5% Saccharomyces cerevisiae respectively) or the mixture of both. Methods and ResultsA total of 57 fish whole intestinal mucosa and contents bacterial...
Tue, 02/28/2017 - 10:55
Abstract AimsSilage is grass, preserved by fermentation and used as winter feed for cattle. The impact of a range of current grass silage preparation practices on the survival of Escherichia coli C600φ3538(Δvtx2::cat) and on the induction, release and infectivity of free phage were investigated. Methods and ResultsWilted and fresh grass samples, from plots with and without slurry application were...
Mon, 02/27/2017 - 00:26
Abstract AimsTo analyse whether some indoleacetic acid (IAA)-secreting plant growth-promoting bacteria can alleviate cadmium (Cd) stress, the role of an Ochrobactrum sp. MGJ11 from rhizosphere of soybean in promoting plant growth, and to evaluate the counteracting Cd effects on soybean. Methods and ResultsOchrobactrum sp. MGJ11 produced 121·2 mg l−1 of IAA. MGJ11 increased soybean root length,...
Sat, 02/25/2017 - 15:35
Abstract AimsThe population of Himalayan region is known to consume variety of fermented and non fermented foods and as a result they have been benefited in terms of overall health, because of the associated beneficial microbes. Therefore, the focus of the present study was to identify new strains of Lactic Acid Bacteria (LAB) from dairy products such as milk (cow, goat, buffalo) and fermented...

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