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Journal of Applied Microbiology

Fri, 05/20/2016 - 12:15
Abstract AimsThe aim of this study was to isolate and identify potentially pathogenic strains of Y. enterocolitica in water samples collected from the upstream section of the Drwęca River in Poland. Methods and ResultsThirty-nine water samples were collected. Strains were isolated, identified with the use of the API®20E test kit (Biomerieux, France) at 37°C, serotyped and subjected to a molecular...
Tue, 05/17/2016 - 09:55
Absract AimsStaphylococcus aureus (S. aureus) produces a wide variety of staphylococcal enterotoxins (SEs) and staphylococcal enterotoxin-like (SEl) proteins, which are the most causative agents of staphylococcal food poisoning. In contrast to classical SEs (SEA to SEE), the relationship between the novel SEs/SEls (SEG to SElX) and staphylococcal food poisoning is not elucidated. This study is...
Thu, 05/12/2016 - 10:45
Abstract The genus Lactococcus comprises twelve species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium (LAB), probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species has been isolated from a large variety of food: meat,...
Mon, 05/09/2016 - 10:41
Abstract AimThe objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. Methods and ResultsThe strains were tested for their ability to inhibit growth of Enterococcus faecalis, Salmonella enterica subsp. enterica serotype Enteritidis, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli,...
Wed, 05/04/2016 - 21:50
Abstract AimTo evaluate the technological and safety properties of Lactobacillus futsaii CS3 and CS5 isolated from Thai fermented shrimp products (Kung-Som) in order to develop a valuable gamma-aminobutyric acid (GABA)-producing starter culture. Methods and ResultsBoth strains showed a high GABA-producing ability (>8 mg ml−1) in MRS broth containing 20 mg ml−1 monosodium glutamate (MSG) for...
Tue, 05/03/2016 - 04:27
Abstract AimsTo investigate the mechanisms leading to an increase in the prevalence of blaCMY-2 conferring resistance to ceftiofur in pigs receiving a feed medicated with chlortetracycline and penicillin and to examine the effect of supplementation with a clay mineral on this phenomenon. Methods and ResultsIn 138 blaCMY-2-positive E. coli isolates from feces of pigs receiving feed supplemented or...
Thu, 04/28/2016 - 12:50
Abstract AimsThe aims of this study were to characterize the antibacterial activity and the chemotype of Litsea cubeba leaf essential oil (EO) harvested in North Vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (Cyprinus carpio) challenged with Aeromonas hydrophila. Methods and ResultsThe EO showed the...
Wed, 03/23/2016 - 06:46
Abstract AimsTo investigate the effects of long-term acid mine drainage (AMD) irrigation on the change of bacterial community and sulfate-reducing bacteria (SRB) in a paddy soil. Methods and resultsThe bacterial community structures were investigated using 454 pyrosequencing, and 98,931 effective sequences were selected for the bacterial diversity analysis. The known dominant phyla in the paddy...
Wed, 03/23/2016 - 06:40
Abstract AimsThe aim of this study was to investigate the potential of bioleaching for the treatment of an environmentally hazardous waste, a blast-furnace flue dust designated Theisen sludge. Methods and resultsBioleaching of Theisen sludge was investigated at acidic conditions with A. ferrooxidans in pure and mixed-species culture with Acidiphilium. In shaking-flask experiments bioleaching...
Wed, 03/23/2016 - 02:40
Abstract AimTo elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks. Methods and ResultsA two-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at 9 farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44.0%) and 24 (96.0%) broiler flocks,...
Tue, 03/22/2016 - 09:10
Abstract AimsThe aim of this study has been to synthesize silver nanoparticles (AgNPs) by an eco-friendly and low-cost method using of the fungi Aspergillus terreus HA1N and Penicillium expansum HA2N as an alternative to chemical procedures mostly requiring drastic experimental conditions emitting toxic chemicals byproduct. Also, this study has been extended to evaluate the effect of AgNPs on the...
Mon, 03/21/2016 - 12:52
Abstract AimsTo investigate the erythromycin resistance patterns and mechanism for Campylobacter isolates in China. Methods and ResultsThe minimum inhibitory concentrations of erythromycin on 858 Chinese Campylobacter isolates were analysed. PCR and DNA sequencing were used to identify mutations in the 23S rRNA and the presence of the ermB gene in the 158 erythromycin resistance isolates (18.4...
Tue, 03/15/2016 - 10:51
Abstract AimsTo characterize Staphylococcus aureus fecal isolates from people suspected to be infected with food poisoning by using antimicrobial susceptibility testing and molecular techniques. Methods and ResultsA total of 340 S. aureus isolates from 6,226 people suspected to be infected with food poisoning were identified and characterized by biochemical methods, antimicrobial susceptibility...
Sat, 03/12/2016 - 02:50
Abstract AimsThe aim of this study was to evaluate which of the three mathematical models (Weibull, Geeraerd and Logistic) makes the best approximation for Hypericin based photosensitization bacterial inactivation. Methods and resultsThe inactivation of common food-borne pathogens Listeria monocytogenes and Bacillus cereus has been analysed. Three models, Weibull, Logistic and Geeraerd, have been...
Sat, 03/12/2016 - 02:36
Abstract AimTo identify core bacterial communities and volatile compounds and to investigate their relationships in Doubanjiang, a Chinese traditional red pepper paste. Methods and ResultsTwo main culture-independent methods, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and MiSeq sequencing technique, were used to investigate bacterial composition. Headspace solid-...
Mon, 03/07/2016 - 10:15
Abstract AimsThe objective of this work was to study the growth potential of E. coli O157:H7 and Salmonella spp. in leafy vegetable extracts at different temperature conditions. Methods and ResultsCocktails of five strains of E. coli O157: H7 and of S. enterica were used. Inoculated aqueous vegetable extracts were incubated at 8, 10, 16 and 20°C during 21 days. Microbial growth was monitored...
Tue, 02/23/2016 - 12:21
Abstract AimsTo investigate whether the Luminex xMAP® Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in Southern China. Methods and ResultsTwo hundred and five Salmonella isolates from diarrhea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes...
Tue, 02/23/2016 - 12:16
Abstract AimsThe aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000-2012 and compare them to the profiles of 43 human strains isolated from 1983-2010, which had been previously studied. Methods and ResultsThe presence of...
Fri, 02/19/2016 - 08:59
Abstract AimsUsing Bayesian methods that do not require the definition of a gold standard, the diagnostic sensitivity and specificity of a real-time polymerase chain reaction (PCR) assay are compared to those of an enriched culture assay for detection of Campylobacter in enriched comminuted chicken samples. Methods and ResultsFood Safety and Inspection Service comminuted chicken samples were...
Mon, 02/08/2016 - 09:00
Abstract AimsThe aim of the study was to determine the prevalence of Mycobacterium bovis (the causative agent of bovine tuberculosis, bTB) in environmental matrices within a French region (Côte d'Or) affected by this zoonotic disease. Methods and ResultsWe report here the development and the use of molecular detection assays based on qPCR (double fluorescent dye labelled probe) to monitor the...
Tue, 02/02/2016 - 13:35
Abstract AimsThis study observed the behavior of probiotics and selected bacterial pathogens co-inoculated into peanut butter during gastrointestinal simulation. Methods and ResultsPeanut butter homogenates co-inoculated with Salmonella/Listeria strains (5 log CFU ml−1) and lyophilized or cultured probiotics (9 log CFU ml−1) were exposed to simulated gastrointestinal conditions for 24 h at 37°C....
Mon, 02/01/2016 - 03:35
Abstract Aimsevaluation of the efficacy of marine LAB, Lactobacillus plantarum AH 78, on growth performance and immune response of Nile tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila. Methods and ResultsMarine probiotic, L. plantarum AH 78, was incorporated in Nile tilapia diets at 0.0, 0.5, 1.0 or 2.0% (w/w). The diets were fed to juvenile Nile tilapia for 40 days, after...
Tue, 01/26/2016 - 05:51
Abstract AimsWe investigated and compared the efficacy of a new apparatus for detaching microorganisms from meat samples. Methods and ResultsThe efficacy of Spindle and stomacher in detaching microorganisms from meat samples was evaluated. Also, evaluation of appropriateness of suspensions generated by both methods for carrying out molecular biological analysis was implemented. A nearly identical...
Wed, 01/20/2016 - 10:31
Abstract AimThe aim of this study was to apply the most sensitive molecular techniques in combination with culture based methods to characterise broiler farms in terms of the timeline (‘appearance’ and ‘pattern’) of Campylobacter contamination prior and post detection in the birds. Methods and ResultsFaecal and environmental samples were collected from 3 broiler farms (2 flocks per farm). RT-PCR...
Wed, 01/20/2016 - 10:03
Summary AimsIn New Zealand, there have been no known cases of foodborne linked to Vibrio vulnificus and shellfish consumption, but two cases of wound infection have been reported. We evaluated the distribution, the effect of environmental parameters, the pheno-genotypic profile, and the growth characteristics of strains isolated from shellfish. Methods and ResultsV. vulnificus was present in 13.6...
Wed, 01/20/2016 - 02:41
Abstract AimsThis study was aimed to identify yeasts and moulds as well as to detect mycotoxin in corn silages in Southern Minas Gerais, Brazil. Methods and ResultsCorn silages from 36 farms were sampled to analyse dry matter, crude protein, ether extract, ash, neutral detergent fibre, non-fibre carbohydrates, and mycotoxins contents, yeasts and moulds population, pH, and temperature values. The...
Thu, 01/07/2016 - 09:20
Abstract AimsTo assess frequencies of the Aspergillus flavus atoxigenic vegetative compatibility group (VCG) YV36, to which the biocontrol agent AF36 belongs, in maize-growing regions of Mexico. Methods and ResultsOver 3,500 Aspergillus flavus isolates recovered from maize agroecosystems in four Mexican states during 2005 through 2008 were subjected to vegetative compatibility analyses based on...
Thu, 01/07/2016 - 09:09
Abstract AimsAssess the diversity of the culturable endophytic bacterial population associated with transgenic and non-transgenic soybean grown in field trial sites in Brazil and characterize them phenotypically and genotypically focusing on characteristics related to plant growth promotion. Methods and ResultsEndophytic bacteria were isolated from roots, stems and leaves of soybean cultivars (...
Thu, 01/07/2016 - 09:07
Abstract AimsSalmonella enterica serovar Enteritidis (S. Enteritidis) encounters mild ethanol stress during its life cycle. However, adaptation to a stressful condition may affect bacterial resistance to subsequent stresses. Hence, this work was undertaken to investigate the influences of ethanol adaptation on stress tolerance of S. Enteritidis. Methods and ResultsS. Enteritidis was subjected to...
Tue, 01/05/2016 - 22:53
Abstract AimsTo determine the prevalence of cross-species sharing of Escherichia coli between healthy dogs and humans living in the same household. Methods and ResultsTwo faecal E. coli isolates from 25 healthy dog-owner pairs and 16 healthy control humans were tested using three fingerprinting methods. The prevalence of within-household sharing of E. coli was 4, 8 and 8% using pulsed-field gel...
Mon, 01/04/2016 - 07:21
Abstract Aims(i) To investigate the enzymatic characterization of α-L-arabinofuranosidase from Thermotoga thermarum DSM5069. (ii) To perform its excellent properties on converting ginsenoside Rc to ginsenoside Rd. Methods and ResultsThe thermostable α-L-arabinofuranosidase (Tt-Afs) gene from Thermotoga thermarum DSM5069 was cloned and overexpressed. Recombinant Tt-Afs was purified, and its...
Fri, 12/18/2015 - 02:32
Abstract AimsThis study investigated the prevalence of Methicillin resistant Staphylococcus aureus (MRSA), their spa-types, and antimicrobial resistance profiles at various steps during commercial pork production from three plants designated as A, B and C. Methods and ResultsOver a period of one year 2,640 samples from three commercial pork plants were obtained on a rotating basis. Sample sources...
Fri, 12/18/2015 - 01:49
Abstract Nisin is a bacteriocin produced by a group of Gram-positive bacteria that belongs to Lactococcus and Streptococcus species. Nisin is classified as a Type A (I) lantibiotic that is synthesized from mRNA and the translated peptide contains several unusual amino acids due to post-translational modifications. Over the past few decades, nisin has been used widely as a food biopreservative....
Wed, 12/16/2015 - 01:34
Abstract AimsTo gain an understanding of the growth kinetics of Staphylococcus aureus to ensure the safety of pork, and to develop a predictive growth model of S. aureus in raw pork, ham, and sausage pork under specific storage time and temperature conditions. Methods and ResultsGrowth of S. aureus was evaluated at 5–40°C using 5–6 replicates in each sampling time in order to capture experimental...
Wed, 12/16/2015 - 01:34
Abstract AimsTo elucidate an entry site of staphylococcal enterotoxin A (SEA), which is a major toxin for staphylococcal foodborne poisoning, into gastrointestinal tissue using a house musk shrew model. Methods and ResultsHouse musk shrews were perorally administered with recombinant SEA and localization of SEA in gastrointestinal tissues was investigated by immunohistochemistry and...
Thu, 12/10/2015 - 07:21
Abstract AimsTo investigate the effects of methyl gallate (MG) on murine macrophages, cytokine production and treatment of Brucella abortus infection using a mouse model. Methods and ResultsMG-treated cells displayed increased F-actin polymerization and modest increase in ERK, JNK and p38α phosphorylation levels. The mice were intraperitoneally infected with Br. abortus and were orally treated...
Sat, 11/28/2015 - 01:13
Abstract AimsThe microbiota surviving sanitation of salmon processing conveyor belts was identified and its growth dynamics further investigated in a model mimicking processing surfaces in such plants. Methods and resultsA diverse microbiota dominated by gram-negative bacteria was isolated after regular sanitation in three salmon processing plants. A cocktail of 14 bacterial isolates representing...
Tue, 11/24/2015 - 09:29
Abstract AimsTo develop a rapid test for thermotolerant Campylobacter in poultry faeces. Methods and ResultsThe reported method is based on immunomagnetic separation and loop-mediated isothermal DNA amplification (IMS/LAMP). This LAMP assay is specific (demonstrated using 10 Campylobacter strains and 13 non-Campylobacter bacterial species) and sensitive (95 % probability of detecting 22 genome...
Tue, 11/24/2015 - 09:26
Abstract AimBased on the results of 19 food microbiology proficiency testing (PT) schemes, this study aimed to assess the laboratory performances, to highlight the main sources of unsatisfactory analytical results and to suggest areas of improvement. Methods and ResultsThe 2009-2015 results of REQUASUD and IPH PT, involving a total of 48 laboratories, were analysed. On average, the laboratories...
Wed, 11/11/2015 - 01:10
Abstract AimsTo increase the knowledge of probiotic effects and potential mechanisms, we report on the use of the zebrafish model to investigate the in vivo colonization ability, as well as the protective effects associated with gut mucosal immune barrier and responses against Aeromonas hydrophila infection of previously characterized probiotic lactic acid bacteria (LAB) strains, Bacillus...
Fri, 11/06/2015 - 02:11
Abstract Campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the European Union, and ranks second in the United States only behind salmonellosis. In Europe, there are about nine million cases of campylobacteriosis every year, making the disease a major public health issue. Human cases are mainly caused by the zoonotic pathogen Campylobacter jejuni. The...
Wed, 11/04/2015 - 09:16
Abstract AimsThis study investigated waterborne opportunistic pathogens (OPs) including potential hosts, and evaluated the use of Legionella spp. for indicating microbial water quality for OPs within a full-scale operating drinking water distribution system (DWDS). Methods and ResultsTo investigate the occurrence of specific microbial pathogens within a major city DWDS we examined large volume (...
Sat, 10/31/2015 - 00:51
Abstract AimsApply Response Surface Methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic microorganism biofilm that creates health problems in the food industry and hospitals. ResultsAn economical medium containing...
Wed, 10/28/2015 - 10:39
Summary Intrinsic resistance to antibiotics is a serious therapeutic problem in the case of many bacterial species. The Gram-positive human pathogen Listeria monocytogenes is intrinsically resistant to broad spectrum cephalosporin antibiotics, which are commonly used in therapy of bacterial infections. Besides three penicillin binding proteins the intrinsic cephalosporin resistome of L....
Fri, 10/23/2015 - 21:06
Abstract AimsThe effect of ohmic heating (OH) in a pilot plant system which had a zig-zag shaped (elbow-type) ohmic heater with five sequential voltage electrodes was investigated on Bacillus cereus spores in a commercial tsuyu sauce. Methods and ResultsThe electrical field was fixed at 26.7 V/cm with an alternating current frequency of 25 kHz. Raw tsuyu sauce (50 L) inoculated with B. cereus...
Mon, 10/19/2015 - 23:22
Abstract AimsTo determine the cause of an outbreak of acute gastroenteritis that occurred on a cruise ship sailing along the Yangzi River from Chongqing to Nanjing, China. Methods and resultsNoroviruses were identified by reverse transcription-PCR (RT-PCR) in rectal swabs from 34 of 54 subjects tested (63.0%). Sequencing and genotyping showed that noroviruses of up to 7 different genotypes...
Mon, 10/19/2015 - 22:55
Abstract AimLittle information is available on a direct comparison of the antibacterial efficacy of LEDs of different peak wavelengths. Thus, the objective of this study was to evaluate the effect of LEDs of three different wavelengths on bacterial inactivation. Methods and ResultsLactobacillus plantarum, Staphylococcus aureus and Vibrio parahaemolyticus were illuminated with 405, 460 and 520 nm...
Mon, 10/19/2015 - 22:36
Abstract AimsProbiotics have been proved to be the most preferred and effective alternative to antibiotics as growth promoter and pathogens inhibitor in poultry industry. In this study Bacillus subtilis CGMCC 1.1086 as a probiotic bacterium was administered in diet and its effects on both the growth performance and the cecal microbiota of broilers were evaluated. Methods and ResultsA total of 240...
Tue, 10/06/2015 - 10:33
Abstract AimsSalmonella enterica subsp. enterica causes salmonellosis in humans and animals. Serovar specific multiple-locus variable-number tandem repeat analysis (MLVA) is widely used for Salmonella surveillance; however, isolates have to be serotyped prior to MLVA typing and only the most common serovars can be typed. We developed a MLVA scheme for high discriminatory typing of Salmonella....
Tue, 10/06/2015 - 10:30
Abstract AimsThe objective of this study was to determine the combined effect of thymoquinone (TQ) and mild heat on C. sakazakii in reconstituted infant formula. Methods and ResultsReconstituted infant formula samples inoculated with a mixture of four C. sakazakii strains (approximately 6.5 log CFU mL−1) were prepared with various concentrations of TQ (0, 5, 10, 20 and 30 mmol L−1) and were...

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