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Journal of Food Safety

Tue, 07/16/2019 - 08:57
Abstract In this research, effects of chitosan coating of brown trout (Salmo trutta fario) fillets containing inulin were determined. Chitosan enriched by vegetable oils (0% Chitosan‐control, 2% acetic acid +Chitosan, 2% sage oil+Chitosan, and 2% mint oil+Chitosan) on shelf life was investigated. In the experiment, fish fillets from the control and the experimental groups were subjected to...
Tue, 07/16/2019 - 08:57
Abstract In this research, effects of chitosan coating of brown trout (Salmo trutta fario) fillets containing inulin were determined. Chitosan enriched by vegetable oils (0% Chitosan‐control, 2% acetic acid +Chitosan, 2% sage oil+Chitosan, and 2% mint oil+Chitosan) on shelf life was investigated. In the experiment, fish fillets from the control and the experimental groups were subjected to...
Sun, 07/14/2019 - 13:23
Abstract The present study tested the antibacterial activity, expressed as minimum bactericidal concentration (MBC), of zinc oxide nanoparticles (ZnO‐NPs), copper oxide nanoparticles (CuO‐NPs) and their combination with or without rosemary, clove or cinnamon extract against Escherichia coli O157:H7 and Listeria monocytogenes. The NPs were characterized by scanning electron microscopy. The sizes...
Sun, 07/14/2019 - 13:23
Abstract The present study tested the antibacterial activity, expressed as minimum bactericidal concentration (MBC), of zinc oxide nanoparticles (ZnO‐NPs), copper oxide nanoparticles (CuO‐NPs) and their combination with or without rosemary, clove or cinnamon extract against Escherichia coli O157:H7 and Listeria monocytogenes. The NPs were characterized by scanning electron microscopy. The sizes...
Tue, 07/09/2019 - 14:30
Abstract In the present work, physicochemical, sensory characteristics and viability of Bifidobacterium bifidum and Lactobacillus casei of probiotic yogurt using dill (Anethum graveolens) essential oil (DEO) (50 and 100 ppm) were investigated at 4°C for 21 days. Alterations in amount of fat and protein of yogurt treatments during storage time were not significant. The viability of B. bifidum and...
Tue, 07/09/2019 - 14:30
Abstract In the present work, physicochemical, sensory characteristics and viability of Bifidobacterium bifidum and Lactobacillus casei of probiotic yogurt using dill (Anethum graveolens) essential oil (DEO) (50 and 100 ppm) were investigated at 4°C for 21 days. Alterations in amount of fat and protein of yogurt treatments during storage time were not significant. The viability of B. bifidum and...
Tue, 07/09/2019 - 05:40
Abstract Today, antibiotic resistance is one of the most crucial problems so that researchers have begun to search for alternatives to antibiotics. Among these alternatives, phage therapy and phytotherapy became prominent. The present study aims to put forward a different approach by combining these two methods. For this purpose, Staphylococcus aureus phages were isolated from commercial...
Tue, 07/09/2019 - 05:40
Abstract Today, antibiotic resistance is one of the most crucial problems so that researchers have begun to search for alternatives to antibiotics. Among these alternatives, phage therapy and phytotherapy became prominent. The present study aims to put forward a different approach by combining these two methods. For this purpose, Staphylococcus aureus phages were isolated from commercial...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Wed, 07/03/2019 - 13:04
Abstract In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and...
Wed, 07/03/2019 - 13:04
Abstract In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and...
Tue, 07/02/2019 - 12:33
Abstract The study aimed at investigating the occurrence and molecular characterization of zoonotic Anisakis larvae in imported, retail‐marketed smoked herring (Clupea harengus) and frozen mackerel (Scomber scombrus) in Egypt. A total of 154 fishes (84 ready‐to‐eat smoked herring and 70 frozen mackerel) were collected. The prevalence of Anisakis larvae Type I in the whole specimen of smoked...
Tue, 07/02/2019 - 12:33
Abstract The study aimed at investigating the occurrence and molecular characterization of zoonotic Anisakis larvae in imported, retail‐marketed smoked herring (Clupea harengus) and frozen mackerel (Scomber scombrus) in Egypt. A total of 154 fishes (84 ready‐to‐eat smoked herring and 70 frozen mackerel) were collected. The prevalence of Anisakis larvae Type I in the whole specimen of smoked...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Thu, 06/27/2019 - 05:08
Abstract This study was focused on determining survival and growth characteristics of Listeria monocytogenes and Staphylococcus aureus on conventional (16.7 mg of sodium/g) and low‐sodium (10.7 mg of sodium/g) bacon. The two types of bacon were inoculated with the either L. monocytogenes or S. aureus stored at 4, 12, or 25°C for up to 7 days. Populations of L. monocytogenes and S. aureus did not...
Thu, 06/27/2019 - 05:08
Abstract This study was focused on determining survival and growth characteristics of Listeria monocytogenes and Staphylococcus aureus on conventional (16.7 mg of sodium/g) and low‐sodium (10.7 mg of sodium/g) bacon. The two types of bacon were inoculated with the either L. monocytogenes or S. aureus stored at 4, 12, or 25°C for up to 7 days. Populations of L. monocytogenes and S. aureus did not...
Tue, 06/25/2019 - 08:43
Abstract Sensory analysis is performed in USFDA testing laboratories to determine the decomposition status of seafood samples. Current alternatives are not always directly comparable to sensory testing. In this study, a novel method was explored using LC‐HRMS with untargeted analysis and a statistical model driven by sensory results via a script written in the R programming language. This allows...
Tue, 06/25/2019 - 08:43
Abstract Sensory analysis is performed in USFDA testing laboratories to determine the decomposition status of seafood samples. Current alternatives are not always directly comparable to sensory testing. In this study, a novel method was explored using LC‐HRMS with untargeted analysis and a statistical model driven by sensory results via a script written in the R programming language. This allows...
Tue, 06/25/2019 - 08:35
Abstract Salmonella‐contaminated raw food is an important source of human food poisoning. Traditional methods for detecting Salmonella in meat involve many time‐consuming and laborious steps, preventing timely and effective control of the pathogen. In this study, a multiplex polymerase chain reaction (PCR) assay that targets serovar‐specific genes such as A7P63_0910 (Salmonella Indiana), sdfI (...
Tue, 06/25/2019 - 08:35
Abstract Salmonella‐contaminated raw food is an important source of human food poisoning. Traditional methods for detecting Salmonella in meat involve many time‐consuming and laborious steps, preventing timely and effective control of the pathogen. In this study, a multiplex polymerase chain reaction (PCR) assay that targets serovar‐specific genes such as A7P63_0910 (Salmonella Indiana), sdfI (...
Mon, 06/24/2019 - 08:33
Abstract The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and commercialized in State of Parana, Brazil. The extraction method carried out with a Romer Mycosep 226 column was found to be efficient due to addition of both diatomaceous earth and centrifuging during the cleaning step. From the total of 84 analyzed samples, 78.6% (n = 66) were found to be...
Mon, 06/24/2019 - 08:33
Abstract The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and commercialized in State of Parana, Brazil. The extraction method carried out with a Romer Mycosep 226 column was found to be efficient due to addition of both diatomaceous earth and centrifuging during the cleaning step. From the total of 84 analyzed samples, 78.6% (n = 66) were found to be...
Mon, 06/24/2019 - 07:45
Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained...
Mon, 06/24/2019 - 07:45
Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained...
Wed, 06/19/2019 - 05:29
Abstract This study aimed to evaluate the ability of the Lactobacillus rhamnosus strain LC‐4 to bind aflatoxin M1 (AFM1) in phosphate‐buffered saline (PBS) and yogurt. Bacterial cells were subjected to heat, acid, and alkali treatments. The AFM1‐binding rate of acid‐treated bacteria was of 78.63 ± 0.52%. The binding of L. rhamnosus LC‐4 to AFM1 was partially reversible and the binding of AFM1...
Wed, 06/19/2019 - 05:29
Abstract This study aimed to evaluate the ability of the Lactobacillus rhamnosus strain LC‐4 to bind aflatoxin M1 (AFM1) in phosphate‐buffered saline (PBS) and yogurt. Bacterial cells were subjected to heat, acid, and alkali treatments. The AFM1‐binding rate of acid‐treated bacteria was of 78.63 ± 0.52%. The binding of L. rhamnosus LC‐4 to AFM1 was partially reversible and the binding of AFM1...
Mon, 06/17/2019 - 13:44
Abstract The detoxification ability of Lactobacillus acidophilus and prebiotics was evaluated by a Plackett‐Burman Design to examine the reduction of concentration and bioaccessibility of aflatoxin B1 (AFB1) and M1 (AFM1) in artificially contaminated whole cow's milk. Six variables were evaluated: AFB1 (3.25–6.0 μg L−1) or AFM1 (1.0–2.0 μg L−1) concentration; incubation time (0–6 h); and inulin,...
Mon, 06/17/2019 - 13:44
Abstract The detoxification ability of Lactobacillus acidophilus and prebiotics was evaluated by a Plackett‐Burman Design to examine the reduction of concentration and bioaccessibility of aflatoxin B1 (AFB1) and M1 (AFM1) in artificially contaminated whole cow's milk. Six variables were evaluated: AFB1 (3.25–6.0 μg L−1) or AFM1 (1.0–2.0 μg L−1) concentration; incubation time (0–6 h); and inulin,...
Tue, 06/11/2019 - 04:54
Abstract This study aimed to functionalize the stainless steel surface with silver and to evaluate its antimicrobial capacity. For this purpose, stainless steel coupons coated or not with silver films were exposed to the presence of Escherichia coli and Staphylococcus aureus. The results of bacterial adhesion on the silver functionalized coupons showed lower counts of adhered S. aureus cells...
Tue, 06/11/2019 - 04:54
Abstract This study aimed to functionalize the stainless steel surface with silver and to evaluate its antimicrobial capacity. For this purpose, stainless steel coupons coated or not with silver films were exposed to the presence of Escherichia coli and Staphylococcus aureus. The results of bacterial adhesion on the silver functionalized coupons showed lower counts of adhered S. aureus cells...
Tue, 06/11/2019 - 04:54
Abstract Concerns over the safety of nitrite and its role in the formation of nitrosamines have aroused considerable interest in developing alternative antimicrobial agents to replace nitrite. Plant‐derived antimicrobial agents could serve against food spoilage and pathogens. For the first time in this study, three semiempirical kinetic models, that is, exponential, homographic, and n‐order...
Tue, 06/11/2019 - 04:54
Abstract Concerns over the safety of nitrite and its role in the formation of nitrosamines have aroused considerable interest in developing alternative antimicrobial agents to replace nitrite. Plant‐derived antimicrobial agents could serve against food spoilage and pathogens. For the first time in this study, three semiempirical kinetic models, that is, exponential, homographic, and n‐order...
Sun, 06/02/2019 - 13:16
Journal of Food Safety, Volume 39, Issue 3, June 2019.
Sun, 06/02/2019 - 13:16
Journal of Food Safety, Volume 39, Issue 3, June 2019.
Sun, 05/26/2019 - 11:56
Abstract The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total...
Sun, 05/26/2019 - 11:56
Abstract The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total...
Thu, 05/23/2019 - 12:34
Abstract Listeria monocytogenes (L. monocytogenes) is a major concern in human food safety. Here, the development of a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) method, targeting the hly A gene of L. monocytogenes, to rapidly detect this important pathogen in food is described. The RT‐LAMP conditions were optimized and the following parameters established: 60 min,...
Thu, 05/23/2019 - 12:34
Abstract Listeria monocytogenes (L. monocytogenes) is a major concern in human food safety. Here, the development of a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) method, targeting the hly A gene of L. monocytogenes, to rapidly detect this important pathogen in food is described. The RT‐LAMP conditions were optimized and the following parameters established: 60 min,...
Thu, 05/23/2019 - 11:24
Abstract The abattoir sector plays a crucial role in controlling foodborne hazards, so insight in the incentives that motivate abattoirs to implement control measures is of great relevance to food safety risk managers and policy makers. Therefore, a cross‐sectional telephone survey was conducted to assess the attitudes and opinions of abattoirs in Belgium and the Netherlands toward preventive...
Thu, 05/23/2019 - 11:24
Abstract The abattoir sector plays a crucial role in controlling foodborne hazards, so insight in the incentives that motivate abattoirs to implement control measures is of great relevance to food safety risk managers and policy makers. Therefore, a cross‐sectional telephone survey was conducted to assess the attitudes and opinions of abattoirs in Belgium and the Netherlands toward preventive...
Wed, 05/22/2019 - 13:00
Abstract Listeria monocytogenes is a major foodborne pathogen that may contaminate liquid egg yolk (LEY). A background microbiota, purified and identified as Enterococcus faecium with a 99.0% probability, was found in pasteurized unsalted LEY. This study was conducted to investigate the thermal resistance of L. monocytogenes and the background microbiota in unsalted and 10% salted LEY at...
Wed, 05/22/2019 - 13:00
Abstract Listeria monocytogenes is a major foodborne pathogen that may contaminate liquid egg yolk (LEY). A background microbiota, purified and identified as Enterococcus faecium with a 99.0% probability, was found in pasteurized unsalted LEY. This study was conducted to investigate the thermal resistance of L. monocytogenes and the background microbiota in unsalted and 10% salted LEY at...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Fri, 05/17/2019 - 15:35
Abstract Pesticide residues in produce are not commonly monitored in developing countries. This study aims to determine whether washing and peeling has an effect on removing pesticide residues from guava fruits. Seventy‐five guava samples were purchased from Thai markets, and analyzed for 67 pesticides. Eighteen pesticides were detected in the guava samples. The incidence of pesticide detection...
Fri, 05/17/2019 - 15:35
Abstract Pesticide residues in produce are not commonly monitored in developing countries. This study aims to determine whether washing and peeling has an effect on removing pesticide residues from guava fruits. Seventy‐five guava samples were purchased from Thai markets, and analyzed for 67 pesticides. Eighteen pesticides were detected in the guava samples. The incidence of pesticide detection...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...

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