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Journal of Microbiological Methods

Sun, 04/15/2018 - 01:26
Publication date: Available online 14 April 2018 Author(s): Ibtissem Djinni, Warda Djoudi, Samiha Souagui, Farida Rabia, Sihem Rahmouni, Ines Mancini, Mouloud Kecha Streptomyces thermoviolaceus SRC3, a newly isolated actinobacterial strain from Algerian river sediments, exhibited a broad activity against various bacterial and yeast human pathogens (Salmonella typhi ATCC 14028...
Wed, 04/04/2018 - 00:45
Publication date: Available online 3 April 2018 Author(s): Jianfa Bai, Valentina Trinetta, Xiaorong Shi, Lance W. Noll, Gabriela Magossi, Wanglong Zheng, Elizabeth P. Porter, Natalia Cernicchiaro, David G. Renter, T.G. Nagaraja Cattle lymph nodes can harbor Salmonella and potentially contaminate beef products. We have developed and validated a new real-time PCR (qPCR)...
Fri, 03/23/2018 - 23:56
Publication date: Available online 23 March 2018 Author(s): Aline Morgan von Hertwig, Anderson S. Sant'Ana, Daniele Sartori, Josué José da Silva, Maristela S. Nascimento, Beatriz Thie Iamanaka, Maria Helena PelegrinelliFungaro, Marta Hiromi Taniwaki Some species from Aspergillus section Nigri are morphologically very similar and altogether have been called A. niger aggregate...
Wed, 03/21/2018 - 23:45
Publication date: Available online 21 March 2018 Author(s): Carla Sebastiani, Ludovica Curcio, Marcella Ciullo, Deborah Cruciani, Silvia Crotti, Cristina Pesca, Martina Torricelli, Martina Sebastianelli, Andrea Felici, Massimo Biagetti Abortion in ruminants represents an important economic concern for farmers. Microbial agents, such as Brucella spp., Chlamydia spp.,...
Thu, 03/15/2018 - 23:22
Publication date: Available online 15 March 2018 Author(s): Siyue Gao, Brooke Pearson, Lili He The existence of pathogenic bacteria in drinking water has been a threat to the safety of human well-being. Traditional methods to detect bacteria are standard plate counts or rapid methods such as ELISA and PCR. However, those methods can be time-consuming or require pre-enrichment when...
Tue, 03/06/2018 - 21:42
Publication date: April 2018 , Volume 147 Author(s): Tatsuya Tominaga Bacteria of the genera Klebsiella and Raoultella, which are present in foods and the natural environment, are associated with health hazards in humans. In the present study, two types of strips-based methods were developed to detect these bacteria simply and quickly. One method used lateral-flow test strips (LFTS) in...
Sun, 03/04/2018 - 21:18
Publication date: March 2018 , Volume 146 Author(s): Bassam A. Elgamoudi, Julian M. Ketley, Victoria Korolik Chemotactic behaviour is an important part of the lifestyle of motile bacteria and enables cells to respond to various environmental stimuli. The Hard Agar Plug (HAP) method is used to study the chemotactic behaviour of bacteria, including the fastidious microaerophile...
Sun, 12/17/2017 - 13:41
Publication date: Available online 17 December 2017 Author(s): Xi-Jun Tang, Ze Yang, Xin-Bin Chen, Wen-Fang Tian, Cheng-Ning Tu, Hai-Bo Wang Salmonella spp./Shigella spp. are often associated with food poisoning and fecal-oral transmission of acute gastroenteritis that requires strict monitoring, especially among people who would handle food and water. In 2014, the National...
Fri, 12/15/2017 - 13:13
Publication date: Available online 12 December 2017 Author(s): Pallop Tankaew, Wanwisa Srisawat, Tawatchai Singhla, Khajornsak Tragoolpua, Yasushi Kataoka, Takuo Sawada, Nattawooti Sthitmatee The ELISA is recognized as an efficient diagnostic tool for antibody detection, but there is no standard ELISA assay for detection of antibodies against hemorrhagic septicemia (HS) in...
Fri, 12/15/2017 - 13:13
Publication date: January 2018 , Volume 144 Author(s): Ariadna Martín, Ana Pérez-Ayala, Fernando Chaves, David Lora, M. Ángeles Orellana Rapid and accurate detection of the pathogens that cause gastrointestinal infection is important for appropriate therapy and proper infection control. This study assesses the performance of a new molecular assay for simultaneous detection of 13...
Fri, 12/15/2017 - 13:13
Publication date: January 2018 , Volume 144 Author(s): Pierre Thouvenot, Guillaume Vales, Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Mylène M. Maury, Alexandra Moura, Marc Lecuit, Alexandre Leclercq This study aimed to evaluate MALDI-TOF MS for species discrimination of Listeria in the context of routine surveillance. MALDI-TOF MS yielded 100% accuracy for the identification...
Fri, 09/01/2017 - 12:48
Publication date: Available online 31 August 2017 Author(s): Maria Cryskely A. Batinga, Jaíne C. dos Santos, Julia T.R. Lima, Maria Fernanda D. Bigotto, Kerstin Muner, Thalita Faita, Rodrigo M. Soares, David A.V. da Silva, Trícia M.F.S. Oliveira, Helena L. Ferreira, Jaqueline A. Diniz, Lara B. Keid Brucella canis, a gram-negative, facultative intracellular and...
Fri, 08/25/2017 - 12:34
Publication date: Available online 24 August 2017 Author(s): Pichayanut Poolperm, Thanya Varinrak, Yasushi Kataoka, Khajornsak Tragoolpua, Takuo Sawada, Nattawooti Sthitmatee Serological tests, such as agglutination and indirect hemagglutination assay (IHA), have been used to identify antibodies against Pasteurella multocida in poultry sera, but none are highly sensitive. An...
Fri, 07/21/2017 - 11:38
Publication date: September 2017 , Volume 140 Author(s): Waldemar Rastawicki, Anna Chróst, Kornelia Gielarowiec Latex agglutination tests (LAT) were developed and evaluated for the rapid detection of LPS antibodies against E. coli serogroup O157, O26, O104, O111 and O145. The latex tests have been proved to be sensitive, fast, easy-to-perform and cost-efficient tools for the screening...
Mon, 06/12/2017 - 09:41
Publication date: Available online 12 June 2017 Author(s): Xiaoping Zhang, Lichuan Guo, Ranran Ma, Lijuan Cong, Zhonghua Wu, Ying Wei, Shijie Xue, Wei Zheng, Saijun Tang Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method...
Wed, 06/07/2017 - 08:36
Publication date: Available online 6 June 2017 Author(s): Rebecca L. Lindsey, L. Garcia-Toledo, D. Fasulo, L.M. Gladney, N. Strockbine Escherichia coli, Escherichia albertii, and Escherichia fergusonii are closely related bacteria that can cause illness in humans, such as bacteremia, urinary tract infections and diarrhea. Current identification strategies for these three species...
Thu, 06/01/2017 - 08:30
Publication date: Available online 31 May 2017 Author(s): Weili Liu, Zhi-Gang Qiu, Min Jin, Jing-Feng Wang, Dong Yang, Zhong-Hai Xiao, Chao Li, Zhao-Kang Yuan, Jun-Wen Li, Qun-Ying Xu, Zhi-Qiang Shen Bacteriophages are widely used to the treatment of drug-resistant bacteria and the improvement of food safety through bacterial lysis. However, the limited investigations...
Sat, 05/27/2017 - 08:26
Publication date: Available online 27 May 2017 Author(s): Qi Wang, Xiao-Juan Zhao, Zi-Wei Wang, Li Liu, Yong-Xin Wei, Xiao Han, Jing Zeng, Wan-Jin Liao Rapid and precise identification of Cronobacter species is important for foodborne pathogen detection, however, commercial biochemical methods can only identify Cronobacter strains to genus level in most cases. To evaluate the...
Thu, 04/27/2017 - 06:38
Publication date: Available online 26 April 2017 Author(s): Kim Ziebell, Linda Chui, Robin King, Suzanne Johnson, Patrick Boerlin, Roger P. Johnson Salmonella enterica subspecies enterica serovar Enteritidis (SE) is one of the most common causes of human salmonellosis and in Canada currently accounts for over 40% of human cases. Reliable subtyping of isolates is required for...
Sun, 04/23/2017 - 06:22
Publication date: Available online 23 April 2017 Author(s): Vidya Srinivasan, Robert D. Stedtfeld, Dieter M. Tourlousse, Samuel W. Baushke, Yu Xin, Sarah M. Miller, Trinh Pham, Jean-Marie Rouillard, Erdogan Gulari, James M. Tiedje, Syed A. Hashsham Parallel detection approaches are of interest to many researchers interested in identifying multiple water and foodborne...
Fri, 04/21/2017 - 06:20
Publication date: Available online 20 April 2017 Author(s): Prem Kumar Periyannan Rajeswari, Lovisa M. Soderberg, Alia Yacoub, Mikael Leijon, Helene Andersson Svahn, Haakan N. Joensson We present a droplet PCR workflow for detection of multiple pathogen DNA biomarkers using fluorescent color-coded Luminex® beads. This strategy enables encoding of multiple singleplex droplet PCRs...
Wed, 04/12/2017 - 06:09
Publication date: Available online 12 April 2017 Author(s): Rahul D. Suryawanshi, Satya Veer Singh Malik, Bhushan Jayarao, Sandeep P. Chaudhari, Emily Savage, Jess Vergis, Nitin V. Kurkure, Sukhadeo B. Barbuddhe, Deepak B. Rawool The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and...
Wed, 03/22/2017 - 02:23
Publication date: Available online 21 March 2017 Author(s): Charlotte Toulouse, Claudia C. Häse, Julia Steuber LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase...
Sun, 03/05/2017 - 23:32
Publication date: Available online 4 March 2017 Author(s): John Dustin Loy, Michael L. Clawson Genotype 2 M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly...
Wed, 02/01/2017 - 18:56
Publication date: Available online 1 February 2017 Author(s): Macarena Varas, Alonso Fariña, Francisco Díaz-Pascual, Javiera Ortíz-Severín, Andrés E. Marcoleta, Miguel L. Allende, Carlos A. Santiviago, Francisco P. Chávez The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune...
Wed, 02/01/2017 - 18:56
Publication date: Available online 1 February 2017 Author(s): Christina Ferrato, Linda Chui, Robin King, Marie Louie Salmonella is one of the most common enteric pathogens related to foodborne illness. Alberta's Provincial Laboratory for Public Health (ProvLab) provides Outbreak and Surveillance support by performing serotyping. The Check&Trace Salmonella™ (CTS) assay (Check...
Mon, 01/16/2017 - 17:19
Publication date: Available online 16 January 2017 Author(s): Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones In this study, the use of skimmed milk flocculation (SMF) to...
Thu, 01/12/2017 - 17:04
Publication date: Available online 12 January 2017 Author(s): Pallop Tankaew, Thawatchai Singh-La, Chatchote Titaram, Veerasak Punyapornwittaya, Preeyanat Vongchan, Takuo Sawada, Nattawooti Sthitmatee Pasteurella multocida causes haemorrhagic septicemia in livestock and wild animals, including elephants. The disease has been reported in Asian elephants in India and Sri Lanka,...
Thu, 12/22/2016 - 14:20
Publication date: Available online 22 December 2016 Author(s): Angela Ma, Linda Chui Three qPCR assays targeting the locus of heat resistance to identify heat resistant clinical Escherichia coli isolates are described. Of 613 isolates, 3 (0.5%) possessed the locus. The assays are a rapid, highly sensitive and specific alternative to screening by heat shock and can be used in food safety...
Tue, 12/20/2016 - 13:55
Publication date: Available online 19 December 2016 Author(s): Romain Briquaire, Rita R. Colwell, Jacques Boncy, Emmanuel Rossignol, Aline Dardy, Isabelle Pandini, François Villeval, Jean-Louis Machuron, Anwar Huq, Shah Rashed, Thierry Vandevelde, Christine Rozand Cholera is now considered to be endemic in Haiti, often with increased incidence during rainy seasons....
Sun, 12/18/2016 - 13:24
Publication date: Available online 18 December 2016 Author(s): Marie Bugarel, Alex Tudor, Guy H Loneragan, Kendra K Nightingale Foodborne illnesses due to Salmonella represent an important public-health concern worldwide. In the United States, a majority of Salmonella infections are associated with a small number of serotypes. Furthermore, some serotypes that are overrepresented...
Sat, 12/10/2016 - 11:57
Publication date: Available online 10 December 2016 Author(s): Tiantian Wang, Sanghyo Kim, Jeong Ho An Loop-mediated isothermal amplification (LAMP) is considered as one of the alternatives to the conventional PCR and it is an inexpensive portable diagnostic system with minimal power consumption. The present work describes the application of LAMP in real-time photon detection and...
Fri, 12/09/2016 - 11:46
Publication date: Available online 8 December 2016 Author(s): Hideyuki Arimitsu, Keiko Sasaki, Takao Tsuji Here, we report the development of an immunochromatographic test strip that can detect heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. Five types of monoclonal antibody (mAb)-producing hybridomas were isolated: three mAbs were A subunit specific and two...
Tue, 12/06/2016 - 11:37
Publication date: Available online 6 December 2016 Author(s): Xiaohong Shi, Xiaoqing Zhang, Qiongqiong Yao, Fengjiao He The rapid detection of microbes is critical in clinical diagnosis and food safety. Culture-dependent assays are the most widely used microbial detection methods, but these assays are time-consuming. In this study, a rapid microbial detection method was proposed...
Sun, 12/04/2016 - 11:21
Publication date: Available online 3 December 2016 Author(s): Chanyong Park, Jinyeop Lee, Yonghee Kim, Jaewon Kim, Jinkee Lee, Sungsu Park Various types of microfluidic systems have been developed to detect bacterial pathogens. However, most of these require enrichment steps that take at least several hours when detecting bacteria that are present with a low number of cells and,...
Thu, 11/17/2016 - 08:52
Publication date: Available online 17 November 2016 Author(s): Lucía Calleros, Laura Betancor, Gregorio Iraola, Alejandra Méndez, Claudia Morsella, Fernando Paolicchi, Silvia Silveyra, Alejandra Velilla, Ruben Pérez Campylobacter fetus is a Gram-negative, microaerophilic bacterium that infects animals and humans. The subspecies Campylobacter fetus subsp. fetus (Cff)...
Wed, 11/09/2016 - 08:11
Publication date: Available online 9 November 2016 Author(s): Lydia-Marie Joubert, Jose AG Ferreira, David A Stevens, Hasan Nazik, Lynette Cegelski Aspergillus fumigatus biofilms consist of a three-dimensional network of cellular hyphae and extracellular matrix. They are involved in infections of immune-compromised individuals, particularly those with cystic fibrosis. These...
Sat, 10/29/2016 - 07:38
Publication date: December 2016 , Volume 131 Author(s): Chandrashekhar Patil, Rahul Suryawanshi, Sunil Koli, Satish Patil Aminocyclopropane-1-carboxylate deaminase (ACCD) producing microorganisms support plant growth under a variety of biotic and abiotic stress conditions such as drought, soil salinity, flooding, heavy metal pollution and phyto-pathogen attack. Available screening...
Sun, 10/16/2016 - 05:09
Publication date: Available online 15 October 2016 Author(s): Xinfeng Liu, Yuyao Guan, Shiliang Cheng, Yidan Huang, Qin Yan, Jun Zhang, Guanjun Huang, Jian Zheng, Tianqiang Liu Vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. It is also a foodborne pathogen that causes diarrhea in humans. The aim...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Igor Viana Brandi, Eliane Macedo Sobrinho Santos, Bruna Mara Aparecida de Carvalho, Carla Adriana Ferreira Durães, Paula Karoline Soares Farias, Roger Shoji Sari, Alex Sander Rodrigues Cangussu, Adalberto Pessoa An efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Deepak B. Rawool, Swapnil P. Doijad, Krupali V. Poharkar, Mamta Negi, Satyajit B. Kale, S.V.S. Malik, Nitin V. Kurkure, Trinad Chakraborty, Sukhadeo B. Barbuddhe A novel multiplex PCR assay was developed to identify genus Listeria, and discriminate Listeria monocytogenes and its major lineages (LI, LII, LIII). This...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Hongsheng Huang, Beverley Phipps-Todd, Tanis McMahon, Catherine L. Elmgren, Cheryl Lutze-Wallace, Zoe A. Todd, Manuel M. Garcia Campylobacter species, particularly thermotolerant Campylobacter spp., such as C. jejuni, are major human foodborne pathogens. Culture methods have been routinely used for the detection of this...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Fernanda S. Nascimento, Yuping Wei-Pridgeon, Michael J. Arrowood, Delynn Moss, Alexandre J. da Silva, Eldin Talundzic, Yvonne Qvarnstrom Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as...
Sat, 10/15/2016 - 05:05
Publication date: December 2016 , Volume 131 Author(s): Maaike J.C. van den Beld, Alexander W. Friedrich, Evert van Zanten, Frans A.G. Reubsaet, Mirjam A.M.D. Kooistra-Smid, John W.A. Rossen An inter-laboratory collaborative trial for the evaluation of diagnostics for detection and identification of Shigella species and Entero-invasive Escherichia coli (EIEC) was performed....
Thu, 06/16/2016 - 14:01
Publication date: Available online 15 June 2016 Author(s): Krunoslav Bojanić, Anne Camilla Midwinter, Jonathan Craig Marshall, Lynn Elizabeth Rogers, Patrick Jon Biggs, Els Acke Campylobacter enteritis in humans is primarily associated with C. jejuni/coli infection. The impact of other Campylobacter spp. is likely to be underestimated due to the bias of culture methods towards...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): Hamouda Elabed, Rim Hamza, Amina Bakhrouf, Kamel Gaddour Even with advances in molecular cloning and DNA transformation, new or alternative methods that permit DNA penetration in Salmonella enterica subspecies enterica serovar Typhimurium are required in order to use this pathogen in biotechnological or medical applications. In this...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): M. Jimenez, H. Bridle We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): William B. Nilsson, Jeffrey W. Turner Vibrio parahaemolyticus is a leading cause of bacterial food-related illness associated with the consumption of undercooked seafood. Only a small subset of strains is pathogenic. Most clinical strains encode for the thermostable direct hemolysin (TDH) and/or the TDH-related hemolysin (TRH). In...
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Hua Yin, Yanlin Ma, Yang Deng, Zhenbo Xu, Junyan Liu, Junfeng Zhao, Jianjun Dong, Junhong Yu, Zongming Chang Genome shuffling is an efficient and promising approach for the rapid improvement of microbial phenotypes. In this study, genome shuffling was applied to enhance the yield of glutathione produced by Saccharomyces...
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Shinya Yonogi, Masashi Kanki, Takahiro Ohnishi, Masami Shiono, Tetsuya Iida, Yuko Kumeda Clostridium perfringens causes food-borne gastroenteritis following the consumption of contaminated food by producing C. perfringens enterotoxin (CPE) in the intestines. Recently, we reported a novel enterotoxin, binary enterotoxin of C....

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