You are here

Journal of Microbiological Methods

Mon, 03/18/2019 - 01:12
Available online 16 March 2019 Author(s): Himadri Tanaya Behera, Anjani Kumar Upadhyay, Vishakha Raina, Lopamudra RayAbstractOptimization of media composition for microbial growth is crucial particularly in industrial processes to obtain the desired end product. The waste from sea food industries includes the non-edible parts of shrimp, crabs and prawns which are rich in chitin as the major cause...
Sun, 03/10/2019 - 01:13
Available online 9 March 2019 Author(s): Reinhard Sting, Tobias Eisenberg, Maja HrubenjaAbstractAnalyses of short subunit gene sequences have been established for taxonomic classification and identification of bacteria and fungi. To produce partial bacterial ribosomal 16S rRNA and rpoB and fungal ribosomal ITS/LSU gene sequences for DNA sequencing, real-time PCR assays supplemented with the...
Wed, 02/27/2019 - 01:12
Available online 25 February 2019 Author(s): Keita Takahashi, Yuki Hanamura, Nagisa Tokunoh, Kohei Kassai, Masaru Matsunishi, Shiori Watanabe, Tsuyoshi Sugiyama, Naoki InoueAbstractEvaluation of the efficacy of vaccine candidates that prevent enteropathogenic and enterohemorrhagic Escherichia coli (EPEC/EHEC) infection in mouse models is difficult due to their limited pathogenicity in mice....
Wed, 02/27/2019 - 01:12
Available online 26 February 2019 Author(s): Daoyi Xi, Fuyi Jing, Qian Liu, Boyang CaoAbstractPlesiomonas shigelloides is widely associated with human diarrheal disease. Research on this pathogen has been hampered by the absence of an effective genetic manipulation system. In the present study, an efficient and precise conjugation transfer procedure, mediated by suicide vector pRE112 was used to...
Mon, 02/25/2019 - 01:11
Available online 20 February 2019 Author(s): Jafar Ali, Naeem Ali, Lei Wang, Hassan Waseem, Gang PanAbstractSynthesis and application of reliable nanoscale materials is a progressive domain and the limelight of modern nanotechnology. Conventional physicochemical approaches for the synthesis of metal nanoparticles have become obsolete owing to costly and hazardous materials. There is a need to...
Sun, 02/17/2019 - 01:13
Available online 15 February 2019 Author(s): Bo Zhang, Yibeibaihan Maimaiti, Chunyan Liu, Jing Li, Haiye Wang, Haojie Lin, Zhaohui Deng, Xinhong Lu, Xin ZhangAbstractObjectiveClinical diagnosis of bloodstream infection diseases depends on the blood culture results. Bacterial identification by traditional methods is time-consuming. This study aimed to utilize molecular beacon-based fluorescence in...
Fri, 02/08/2019 - 01:12
Available online 6 February 2019 Author(s): Xinjie Song, Shruti Shukla, Myunghee KimAbstractCronobacter species are foodborne pathogens that pose a high risk in infant formula and can cause fatality rates of 40–80% in infected infants. To develop a rapid and easy detection method for Cronobacter species, especially in powdered infant formula (PIF), an immunoliposome-based immunochromatographic...
Wed, 01/30/2019 - 01:10
Available online 29 January 2019 Author(s): Jinqiang Hu, Runna Huang, Yanting Sun, Xiangke Wei, Yi Wang, Chunpeng Jiang, Yao Geng, Xincheng Sun, Jianzhou Jing, Hui Gao, Zhangcun Wang, Caiwen DongAbstractSalmonella Typhimurium (S. Typhimurium) can cause serious foodborne diseases. In this study, an assay combining recombinase polymerase amplification (RPA) with lateral flow dipsticks (LFD) was...
Wed, 01/23/2019 - 01:08
Available online 22 January 2019 Author(s): Richard M. Gell, Ignazio CarboneAbstractAflatoxins are mycotoxins that contaminate agricultural products when infected by toxigenic Aspergillus flavus. Methods for quantifying aflatoxin from culture using chromatography are available but are not optimized for population studies. We provide details of a method for preparation and quantitation of...
Tue, 01/15/2019 - 01:04
Available online 11 January 2019 Author(s): Al Serhat, Gökhan Kürşad Incili, Aytac Akcay, Ahmet KolumanAbstractAlthough rapid detection kits continue to be developed and used, the classical culture method is still accepted as a gold standard. Therefore accelerating the classical culture methods safely is important for the detection of Campylobacter. The aim of this study is to design and compare...
Sat, 01/12/2019 - 01:09
Available online 10 January 2019 Author(s): D. Nestor, S. Thulin Hedberg, M. Lignell, S. Skovbjerg, P. Mölling, M. SundqvistAbstractPurposeMolecular methods provide fast and accurate detection of both bacteria and viruses in the cerebrospinal fluid (CSF) causing infection in the central nervous system (CNS). In the present study we evaluated the bacterial detection performance of the fully...
Thu, 01/10/2019 - 01:09
Available online 8 January 2019 Author(s): Jaleh Khanifar, Reza Haji Hosseini, Rohoallah Kazemi, Mahdi Fasihi Ramandi, Jafar Amani, Ali Hatef SalmanianAbstractOne of highly effective methods for prevention and control of Entrohemorragic Esherichia coli (EHEC) infections is to use vaccination against extremely immunogenic part of attachment factors. In this study rEIT (EspA, Intimin, Tir) was...
Tue, 12/25/2018 - 01:10
Available online 23 December 2018 Author(s): Maria Dullaert-de Boer, Richard F. de Boer, Annel Lameijer, Elles Sterne, Ben Skidmore, Anita W.M. Suijkerbuijk, Max Heck, Adri G.M. van der ZandenAbstractSelenite enrichment broth (SEB) is used to optimize the recovery of Salmonella enterica subspecies enterica from stool samples. Compared to a direct culture approach, it enhances culture yield by...
Fri, 12/21/2018 - 01:13
Available online 19 December 2018 Author(s): Karanth Padyana Anupama, Kundar Deeksha, Ariga Deeksha, Iddya Karunasagar, Indrani Karunasagar, Biswajit MaitiAbstractVibrio parahaemolyticus is one of the important foodborne pathogens is of public health concern due to the emergence of pandemic strains causing disease outbreaks worldwide. We evaluated the DNA based colony hybridization technique for...
Thu, 12/20/2018 - 01:03
Available online 18 December 2018 Author(s): Sharda Prasad Awasthi, Nityananda Chowdhury, Sucharit Basu Neogi, Atsushi Hinenoya, Noritoshi Hatanaka, Goutam Chowdhury, T. Ramamurthy, Shinji YamasakiAbstractVibrio cholerae strains producing cholera toxin (CT) and toxin co-regulated pilus (TCP) and belonging to O1 and O139 serogroups are responsible for cholera. However, non-CT producing V. cholerae...
Thu, 12/20/2018 - 01:03
Available online 18 December 2018 Author(s): Mélodie Langlais, Alexandre Thibodeau, Philippe FravaloAbstractThe bacterial short pre-enrichment culture step is important for the proper detection and isolation of Salmonella spp. from pig feces. Using metagenomics, we showed that pre-enrichment of Salmonella was favored not only by inhibiting the growth of competing bacteria but also by increasing...
Thu, 12/20/2018 - 01:03
Available online 18 December 2018 Author(s): Yunyun Geng, Guanhui Liu, Libing Liu, Qiaoen Deng, Liwei Zhao, Xiao Xia Sun, Jinfeng Wang, Baohua Zhao, Jianchang WangAbstractCampylobacter jejuni (C. jejuni), a foodborne pathogen, is a major contributor to human bacterial gastroenteritis worldwide and detrimental to public health. It is crucial for initiating appropriate outbreak control strategies...
Wed, 12/05/2018 - 01:08
Available online 22 November 2018 Author(s): Jamal Farheen Begum, Murugesh Tamilarasi, Periaswamy Pushpakanth, Dananjeyan BalachandarAbstractPlant-associated bacteria produce quorum sensing (QS) signals for community (biofilm) formation and functioning in the rhizosphere. The QS-positive biofilm-forming rhizobacteria that excel benefits to the plants are now gaining increase importance for...
Wed, 12/05/2018 - 01:08
Available online 28 November 2018 Author(s): Wansadaj Jaroenram, Paola Cecere, Pier Paolo PompaAbstractLoop-mediated isothermal amplification (LAMP) can amplify DNA specifically and sensitively. Under minimal buffering conditions, it produces hydrogen ions that lower the pH of the solution upon DNA amplification. This characteristic was applied to visually detect amplified DNA of Escherichia coli...
Wed, 12/05/2018 - 01:08
Publication date: December 2018 , Volume 155Author(s): Zhaojie Li, Shouguang Chen, Chenggang Xu, Lingyan Ju, Fengling LiAbstractVibrio parahaemolyticus which naturally inhabits marine and estuarine environment represents pathogenic strains (virulence genes tdh or trh positive) and non-pathogenic strains (virulence genes negative). In this study, a rapid method for subtyping pathogenic and non-...
Wed, 12/05/2018 - 01:08
Publication date: December 2018 , Volume 155Author(s): Shuiping Hou, Xinwei Wu, Yong Zhou, Peng He, Shouyi ChenAbstractWe investigated the efficacy of syringe filters and membrane filters with different pore sizes for recovering Campylobacter. A syringe filter with a 0.45 μm pore size achieved the highest recovery rate (0.29%), while polycarbonate membrane filters with pore sizes of 0.6 μm and 0....
Wed, 12/05/2018 - 01:08
Publication date: December 2018 , Volume 155Author(s): Durga Prasad Das, Satya Veer Singh Malik, Radhakrishna Sahu, Jay Prakash Yadav, Deepak B. Rawool, Sukhadeo B. BarbuddheAbstractWe developed the com1 gene based loop-mediated isothermal amplification (LAMP) assay for the detection of Coxiella burnetii and validated it by screening DNA isolated from serum samples collected from animals and...
Wed, 12/05/2018 - 01:08
Available online 3 December 2018 Author(s): Triveni Kalleshamurthy, Chaithra Yaranna, Ranjitha Shekar, Krithiga Natesan, Swati Sahay, Bibek Ranjan Shome, Habibur Rahman, Sukhadeo B. Barbuddhe, Nagendra Nath Barman, Sailendra Kumar Das, Rajeswari ShomeAbstractBrucellosis in pigs, caused by the bacterium Brucella suis, is an important zoonotic infection. In the present study, fluorescence...
Sun, 04/15/2018 - 01:26
Publication date: Available online 14 April 2018 Author(s): Ibtissem Djinni, Warda Djoudi, Samiha Souagui, Farida Rabia, Sihem Rahmouni, Ines Mancini, Mouloud Kecha Streptomyces thermoviolaceus SRC3, a newly isolated actinobacterial strain from Algerian river sediments, exhibited a broad activity against various bacterial and yeast human pathogens (Salmonella typhi ATCC 14028...
Wed, 04/04/2018 - 00:45
Publication date: Available online 3 April 2018 Author(s): Jianfa Bai, Valentina Trinetta, Xiaorong Shi, Lance W. Noll, Gabriela Magossi, Wanglong Zheng, Elizabeth P. Porter, Natalia Cernicchiaro, David G. Renter, T.G. Nagaraja Cattle lymph nodes can harbor Salmonella and potentially contaminate beef products. We have developed and validated a new real-time PCR (qPCR)...
Fri, 03/23/2018 - 23:56
Publication date: Available online 23 March 2018 Author(s): Aline Morgan von Hertwig, Anderson S. Sant'Ana, Daniele Sartori, Josué José da Silva, Maristela S. Nascimento, Beatriz Thie Iamanaka, Maria Helena PelegrinelliFungaro, Marta Hiromi Taniwaki Some species from Aspergillus section Nigri are morphologically very similar and altogether have been called A. niger aggregate...
Wed, 03/21/2018 - 23:45
Publication date: Available online 21 March 2018 Author(s): Carla Sebastiani, Ludovica Curcio, Marcella Ciullo, Deborah Cruciani, Silvia Crotti, Cristina Pesca, Martina Torricelli, Martina Sebastianelli, Andrea Felici, Massimo Biagetti Abortion in ruminants represents an important economic concern for farmers. Microbial agents, such as Brucella spp., Chlamydia spp.,...
Thu, 03/15/2018 - 23:22
Publication date: Available online 15 March 2018 Author(s): Siyue Gao, Brooke Pearson, Lili He The existence of pathogenic bacteria in drinking water has been a threat to the safety of human well-being. Traditional methods to detect bacteria are standard plate counts or rapid methods such as ELISA and PCR. However, those methods can be time-consuming or require pre-enrichment when...
Tue, 03/06/2018 - 21:42
Publication date: April 2018 , Volume 147 Author(s): Tatsuya Tominaga Bacteria of the genera Klebsiella and Raoultella, which are present in foods and the natural environment, are associated with health hazards in humans. In the present study, two types of strips-based methods were developed to detect these bacteria simply and quickly. One method used lateral-flow test strips (LFTS) in...
Sun, 03/04/2018 - 21:18
Publication date: March 2018 , Volume 146 Author(s): Bassam A. Elgamoudi, Julian M. Ketley, Victoria Korolik Chemotactic behaviour is an important part of the lifestyle of motile bacteria and enables cells to respond to various environmental stimuli. The Hard Agar Plug (HAP) method is used to study the chemotactic behaviour of bacteria, including the fastidious microaerophile...
Sun, 12/17/2017 - 13:41
Publication date: Available online 17 December 2017 Author(s): Xi-Jun Tang, Ze Yang, Xin-Bin Chen, Wen-Fang Tian, Cheng-Ning Tu, Hai-Bo Wang Salmonella spp./Shigella spp. are often associated with food poisoning and fecal-oral transmission of acute gastroenteritis that requires strict monitoring, especially among people who would handle food and water. In 2014, the National...
Fri, 12/15/2017 - 13:13
Publication date: Available online 12 December 2017 Author(s): Pallop Tankaew, Wanwisa Srisawat, Tawatchai Singhla, Khajornsak Tragoolpua, Yasushi Kataoka, Takuo Sawada, Nattawooti Sthitmatee The ELISA is recognized as an efficient diagnostic tool for antibody detection, but there is no standard ELISA assay for detection of antibodies against hemorrhagic septicemia (HS) in...
Fri, 12/15/2017 - 13:13
Publication date: January 2018 , Volume 144 Author(s): Ariadna Martín, Ana Pérez-Ayala, Fernando Chaves, David Lora, M. Ángeles Orellana Rapid and accurate detection of the pathogens that cause gastrointestinal infection is important for appropriate therapy and proper infection control. This study assesses the performance of a new molecular assay for simultaneous detection of 13...
Fri, 12/15/2017 - 13:13
Publication date: January 2018 , Volume 144 Author(s): Pierre Thouvenot, Guillaume Vales, Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Mylène M. Maury, Alexandra Moura, Marc Lecuit, Alexandre Leclercq This study aimed to evaluate MALDI-TOF MS for species discrimination of Listeria in the context of routine surveillance. MALDI-TOF MS yielded 100% accuracy for the identification...
Fri, 09/01/2017 - 12:48
Publication date: Available online 31 August 2017 Author(s): Maria Cryskely A. Batinga, Jaíne C. dos Santos, Julia T.R. Lima, Maria Fernanda D. Bigotto, Kerstin Muner, Thalita Faita, Rodrigo M. Soares, David A.V. da Silva, Trícia M.F.S. Oliveira, Helena L. Ferreira, Jaqueline A. Diniz, Lara B. Keid Brucella canis, a gram-negative, facultative intracellular and...
Fri, 08/25/2017 - 12:34
Publication date: Available online 24 August 2017 Author(s): Pichayanut Poolperm, Thanya Varinrak, Yasushi Kataoka, Khajornsak Tragoolpua, Takuo Sawada, Nattawooti Sthitmatee Serological tests, such as agglutination and indirect hemagglutination assay (IHA), have been used to identify antibodies against Pasteurella multocida in poultry sera, but none are highly sensitive. An...
Fri, 07/21/2017 - 11:38
Publication date: September 2017 , Volume 140 Author(s): Waldemar Rastawicki, Anna Chróst, Kornelia Gielarowiec Latex agglutination tests (LAT) were developed and evaluated for the rapid detection of LPS antibodies against E. coli serogroup O157, O26, O104, O111 and O145. The latex tests have been proved to be sensitive, fast, easy-to-perform and cost-efficient tools for the screening...
Mon, 06/12/2017 - 09:41
Publication date: Available online 12 June 2017 Author(s): Xiaoping Zhang, Lichuan Guo, Ranran Ma, Lijuan Cong, Zhonghua Wu, Ying Wei, Shijie Xue, Wei Zheng, Saijun Tang Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method...
Wed, 06/07/2017 - 08:36
Publication date: Available online 6 June 2017 Author(s): Rebecca L. Lindsey, L. Garcia-Toledo, D. Fasulo, L.M. Gladney, N. Strockbine Escherichia coli, Escherichia albertii, and Escherichia fergusonii are closely related bacteria that can cause illness in humans, such as bacteremia, urinary tract infections and diarrhea. Current identification strategies for these three species...
Thu, 06/01/2017 - 08:30
Publication date: Available online 31 May 2017 Author(s): Weili Liu, Zhi-Gang Qiu, Min Jin, Jing-Feng Wang, Dong Yang, Zhong-Hai Xiao, Chao Li, Zhao-Kang Yuan, Jun-Wen Li, Qun-Ying Xu, Zhi-Qiang Shen Bacteriophages are widely used to the treatment of drug-resistant bacteria and the improvement of food safety through bacterial lysis. However, the limited investigations...
Sat, 05/27/2017 - 08:26
Publication date: Available online 27 May 2017 Author(s): Qi Wang, Xiao-Juan Zhao, Zi-Wei Wang, Li Liu, Yong-Xin Wei, Xiao Han, Jing Zeng, Wan-Jin Liao Rapid and precise identification of Cronobacter species is important for foodborne pathogen detection, however, commercial biochemical methods can only identify Cronobacter strains to genus level in most cases. To evaluate the...
Thu, 04/27/2017 - 06:38
Publication date: Available online 26 April 2017 Author(s): Kim Ziebell, Linda Chui, Robin King, Suzanne Johnson, Patrick Boerlin, Roger P. Johnson Salmonella enterica subspecies enterica serovar Enteritidis (SE) is one of the most common causes of human salmonellosis and in Canada currently accounts for over 40% of human cases. Reliable subtyping of isolates is required for...
Sun, 04/23/2017 - 06:22
Publication date: Available online 23 April 2017 Author(s): Vidya Srinivasan, Robert D. Stedtfeld, Dieter M. Tourlousse, Samuel W. Baushke, Yu Xin, Sarah M. Miller, Trinh Pham, Jean-Marie Rouillard, Erdogan Gulari, James M. Tiedje, Syed A. Hashsham Parallel detection approaches are of interest to many researchers interested in identifying multiple water and foodborne...
Fri, 04/21/2017 - 06:20
Publication date: Available online 20 April 2017 Author(s): Prem Kumar Periyannan Rajeswari, Lovisa M. Soderberg, Alia Yacoub, Mikael Leijon, Helene Andersson Svahn, Haakan N. Joensson We present a droplet PCR workflow for detection of multiple pathogen DNA biomarkers using fluorescent color-coded Luminex® beads. This strategy enables encoding of multiple singleplex droplet PCRs...
Wed, 04/12/2017 - 06:09
Publication date: Available online 12 April 2017 Author(s): Rahul D. Suryawanshi, Satya Veer Singh Malik, Bhushan Jayarao, Sandeep P. Chaudhari, Emily Savage, Jess Vergis, Nitin V. Kurkure, Sukhadeo B. Barbuddhe, Deepak B. Rawool The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and...
Wed, 03/22/2017 - 02:23
Publication date: Available online 21 March 2017 Author(s): Charlotte Toulouse, Claudia C. Häse, Julia Steuber LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase...
Sun, 03/05/2017 - 23:32
Publication date: Available online 4 March 2017 Author(s): John Dustin Loy, Michael L. Clawson Genotype 2 M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly...
Wed, 02/01/2017 - 18:56
Publication date: Available online 1 February 2017 Author(s): Macarena Varas, Alonso Fariña, Francisco Díaz-Pascual, Javiera Ortíz-Severín, Andrés E. Marcoleta, Miguel L. Allende, Carlos A. Santiviago, Francisco P. Chávez The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune...
Wed, 02/01/2017 - 18:56
Publication date: Available online 1 February 2017 Author(s): Christina Ferrato, Linda Chui, Robin King, Marie Louie Salmonella is one of the most common enteric pathogens related to foodborne illness. Alberta's Provincial Laboratory for Public Health (ProvLab) provides Outbreak and Surveillance support by performing serotyping. The Check&Trace Salmonella™ (CTS) assay (Check...
Mon, 01/16/2017 - 17:19
Publication date: Available online 16 January 2017 Author(s): Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones In this study, the use of skimmed milk flocculation (SMF) to...

Pages