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Journal of Microbiological Methods

Sun, 03/05/2017 - 23:32
Publication date: Available online 4 March 2017 Author(s): John Dustin Loy, Michael L. Clawson Genotype 2 M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly...
Wed, 02/01/2017 - 18:56
Publication date: Available online 1 February 2017 Author(s): Macarena Varas, Alonso Fariña, Francisco Díaz-Pascual, Javiera Ortíz-Severín, Andrés E. Marcoleta, Miguel L. Allende, Carlos A. Santiviago, Francisco P. Chávez The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune...
Wed, 02/01/2017 - 18:56
Publication date: Available online 1 February 2017 Author(s): Christina Ferrato, Linda Chui, Robin King, Marie Louie Salmonella is one of the most common enteric pathogens related to foodborne illness. Alberta's Provincial Laboratory for Public Health (ProvLab) provides Outbreak and Surveillance support by performing serotyping. The Check&Trace Salmonella™ (CTS) assay (Check...
Mon, 01/16/2017 - 17:19
Publication date: Available online 16 January 2017 Author(s): Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones In this study, the use of skimmed milk flocculation (SMF) to...
Thu, 01/12/2017 - 17:04
Publication date: Available online 12 January 2017 Author(s): Pallop Tankaew, Thawatchai Singh-La, Chatchote Titaram, Veerasak Punyapornwittaya, Preeyanat Vongchan, Takuo Sawada, Nattawooti Sthitmatee Pasteurella multocida causes haemorrhagic septicemia in livestock and wild animals, including elephants. The disease has been reported in Asian elephants in India and Sri Lanka,...
Thu, 12/22/2016 - 14:20
Publication date: Available online 22 December 2016 Author(s): Angela Ma, Linda Chui Three qPCR assays targeting the locus of heat resistance to identify heat resistant clinical Escherichia coli isolates are described. Of 613 isolates, 3 (0.5%) possessed the locus. The assays are a rapid, highly sensitive and specific alternative to screening by heat shock and can be used in food safety...
Tue, 12/20/2016 - 13:55
Publication date: Available online 19 December 2016 Author(s): Romain Briquaire, Rita R. Colwell, Jacques Boncy, Emmanuel Rossignol, Aline Dardy, Isabelle Pandini, François Villeval, Jean-Louis Machuron, Anwar Huq, Shah Rashed, Thierry Vandevelde, Christine Rozand Cholera is now considered to be endemic in Haiti, often with increased incidence during rainy seasons....
Sun, 12/18/2016 - 13:24
Publication date: Available online 18 December 2016 Author(s): Marie Bugarel, Alex Tudor, Guy H Loneragan, Kendra K Nightingale Foodborne illnesses due to Salmonella represent an important public-health concern worldwide. In the United States, a majority of Salmonella infections are associated with a small number of serotypes. Furthermore, some serotypes that are overrepresented...
Sat, 12/10/2016 - 11:57
Publication date: Available online 10 December 2016 Author(s): Tiantian Wang, Sanghyo Kim, Jeong Ho An Loop-mediated isothermal amplification (LAMP) is considered as one of the alternatives to the conventional PCR and it is an inexpensive portable diagnostic system with minimal power consumption. The present work describes the application of LAMP in real-time photon detection and...
Fri, 12/09/2016 - 11:46
Publication date: Available online 8 December 2016 Author(s): Hideyuki Arimitsu, Keiko Sasaki, Takao Tsuji Here, we report the development of an immunochromatographic test strip that can detect heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. Five types of monoclonal antibody (mAb)-producing hybridomas were isolated: three mAbs were A subunit specific and two...
Tue, 12/06/2016 - 11:37
Publication date: Available online 6 December 2016 Author(s): Xiaohong Shi, Xiaoqing Zhang, Qiongqiong Yao, Fengjiao He The rapid detection of microbes is critical in clinical diagnosis and food safety. Culture-dependent assays are the most widely used microbial detection methods, but these assays are time-consuming. In this study, a rapid microbial detection method was proposed...
Sun, 12/04/2016 - 11:21
Publication date: Available online 3 December 2016 Author(s): Chanyong Park, Jinyeop Lee, Yonghee Kim, Jaewon Kim, Jinkee Lee, Sungsu Park Various types of microfluidic systems have been developed to detect bacterial pathogens. However, most of these require enrichment steps that take at least several hours when detecting bacteria that are present with a low number of cells and,...
Thu, 11/17/2016 - 08:52
Publication date: Available online 17 November 2016 Author(s): Lucía Calleros, Laura Betancor, Gregorio Iraola, Alejandra Méndez, Claudia Morsella, Fernando Paolicchi, Silvia Silveyra, Alejandra Velilla, Ruben Pérez Campylobacter fetus is a Gram-negative, microaerophilic bacterium that infects animals and humans. The subspecies Campylobacter fetus subsp. fetus (Cff)...
Wed, 11/09/2016 - 08:11
Publication date: Available online 9 November 2016 Author(s): Lydia-Marie Joubert, Jose AG Ferreira, David A Stevens, Hasan Nazik, Lynette Cegelski Aspergillus fumigatus biofilms consist of a three-dimensional network of cellular hyphae and extracellular matrix. They are involved in infections of immune-compromised individuals, particularly those with cystic fibrosis. These...
Sat, 10/29/2016 - 07:38
Publication date: December 2016 , Volume 131 Author(s): Chandrashekhar Patil, Rahul Suryawanshi, Sunil Koli, Satish Patil Aminocyclopropane-1-carboxylate deaminase (ACCD) producing microorganisms support plant growth under a variety of biotic and abiotic stress conditions such as drought, soil salinity, flooding, heavy metal pollution and phyto-pathogen attack. Available screening...
Sun, 10/16/2016 - 05:09
Publication date: Available online 15 October 2016 Author(s): Xinfeng Liu, Yuyao Guan, Shiliang Cheng, Yidan Huang, Qin Yan, Jun Zhang, Guanjun Huang, Jian Zheng, Tianqiang Liu Vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. It is also a foodborne pathogen that causes diarrhea in humans. The aim...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Igor Viana Brandi, Eliane Macedo Sobrinho Santos, Bruna Mara Aparecida de Carvalho, Carla Adriana Ferreira Durães, Paula Karoline Soares Farias, Roger Shoji Sari, Alex Sander Rodrigues Cangussu, Adalberto Pessoa An efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Deepak B. Rawool, Swapnil P. Doijad, Krupali V. Poharkar, Mamta Negi, Satyajit B. Kale, S.V.S. Malik, Nitin V. Kurkure, Trinad Chakraborty, Sukhadeo B. Barbuddhe A novel multiplex PCR assay was developed to identify genus Listeria, and discriminate Listeria monocytogenes and its major lineages (LI, LII, LIII). This...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Hongsheng Huang, Beverley Phipps-Todd, Tanis McMahon, Catherine L. Elmgren, Cheryl Lutze-Wallace, Zoe A. Todd, Manuel M. Garcia Campylobacter species, particularly thermotolerant Campylobacter spp., such as C. jejuni, are major human foodborne pathogens. Culture methods have been routinely used for the detection of this...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Fernanda S. Nascimento, Yuping Wei-Pridgeon, Michael J. Arrowood, Delynn Moss, Alexandre J. da Silva, Eldin Talundzic, Yvonne Qvarnstrom Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as...
Sat, 10/15/2016 - 05:05
Publication date: December 2016 , Volume 131 Author(s): Maaike J.C. van den Beld, Alexander W. Friedrich, Evert van Zanten, Frans A.G. Reubsaet, Mirjam A.M.D. Kooistra-Smid, John W.A. Rossen An inter-laboratory collaborative trial for the evaluation of diagnostics for detection and identification of Shigella species and Entero-invasive Escherichia coli (EIEC) was performed....
Thu, 06/16/2016 - 14:01
Publication date: Available online 15 June 2016 Author(s): Krunoslav Bojanić, Anne Camilla Midwinter, Jonathan Craig Marshall, Lynn Elizabeth Rogers, Patrick Jon Biggs, Els Acke Campylobacter enteritis in humans is primarily associated with C. jejuni/coli infection. The impact of other Campylobacter spp. is likely to be underestimated due to the bias of culture methods towards...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): Hamouda Elabed, Rim Hamza, Amina Bakhrouf, Kamel Gaddour Even with advances in molecular cloning and DNA transformation, new or alternative methods that permit DNA penetration in Salmonella enterica subspecies enterica serovar Typhimurium are required in order to use this pathogen in biotechnological or medical applications. In this...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): M. Jimenez, H. Bridle We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): William B. Nilsson, Jeffrey W. Turner Vibrio parahaemolyticus is a leading cause of bacterial food-related illness associated with the consumption of undercooked seafood. Only a small subset of strains is pathogenic. Most clinical strains encode for the thermostable direct hemolysin (TDH) and/or the TDH-related hemolysin (TRH). In...
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Hua Yin, Yanlin Ma, Yang Deng, Zhenbo Xu, Junyan Liu, Junfeng Zhao, Jianjun Dong, Junhong Yu, Zongming Chang Genome shuffling is an efficient and promising approach for the rapid improvement of microbial phenotypes. In this study, genome shuffling was applied to enhance the yield of glutathione produced by Saccharomyces...
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Shinya Yonogi, Masashi Kanki, Takahiro Ohnishi, Masami Shiono, Tetsuya Iida, Yuko Kumeda Clostridium perfringens causes food-borne gastroenteritis following the consumption of contaminated food by producing C. perfringens enterotoxin (CPE) in the intestines. Recently, we reported a novel enterotoxin, binary enterotoxin of C....
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Nadim Ahamad, Dhirendra S. Katti Bacterial lipopolysaccharide (LPS) has been widely used as an antigen and adjuvant in immunological applications. Amongst the methods developed for extraction of LPS, hot phenol extraction (HPE) method is the gold standard. However, the HPE method provides poor yield of LPS (~4.5% by weight), is...
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Davide Porcellato, Judith Narvhus, Siv Borghild Skeie Droplet digital PCR (ddPCR) is one of the newest and most promising methods for the detection and quantification of molecular targets by PCR. Here, we optimized and used a new ddPCR assay for the detection and quantification of the Bacillus cereus group in milk. We also compared...
Mon, 03/21/2016 - 12:03
Publication date: Available online 21 March 2016 Author(s): Natacha Laprade, Michel Cloutier, David R. Lapen, Edward Topp, Graham Wilkes, Richard Villemur, Izhar U.H. Khan Campylobacter species are one of the leading causes of bacterial gastroenteritis in humans worldwide. This twofold study was sought to: i) develop and optimize four single-tube multiplex PCR (mPCR) assays...
Fri, 03/18/2016 - 11:50
Publication date: Available online 18 March 2016 Author(s): Alexandra Duarte, Tomasz Seliwiorstow, William G. Miller, Lieven De Zutter, Mieke Uyttendaele, Katelijne Dierick, Nadine Botteldoorn The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. C. jejuni (n=94) and C. coli (n=52) isolated...
Fri, 03/04/2016 - 10:03
Publication date: Available online 4 March 2016 Author(s): Megan E. Meek, Frances M. Van Dolah Abstract Phytoplankton rarely occur as unialgal populations. Therefore, to study species-specific protein expression, indicative of physiological status in natural populations, methods are needed that will both assay for a protein of interest and identify the species expressing it. Here we...
Mon, 02/08/2016 - 04:34
Publication date: Available online 6 February 2016 Author(s): B. Kramer, J. Thielmann Rapid microbiological methods to assess cell physiological properties of microorganisms are gaining interest in the elucidation of the effect of antimicrobial agents or physical inactivation. Fluorescent probes combined with flow cytometry or microplate assays provide information about cellular targets...
Fri, 02/05/2016 - 04:09
Publication date: Available online 4 February 2016 Author(s): Bongkot Ngamsom, Maria J. Lopez-Martinez, Jean-Claude Raymond, Patrick Broyer, Pradip Patel, Nicole Pamme Pathogen analysis in food samples routinely involves lengthy growth-based pre-enrichment and selective enrichment of food matrices to increase the ratio of pathogen to background flora. Similarly, for blood culture...
Fri, 02/05/2016 - 04:09
Publication date: Available online 4 February 2016 Author(s): Judit K. Kovács, Györgyi Horváth, Monika Kerényi, Béla Kocsis, Levente Emődy, György Schneider Direct bioautography is a useful method to identify antimicrobial compounds with potential therapeutic importance. Because of technical limitations till now, it has been applied only for aerobic bacteria. In this work we...
Thu, 02/04/2016 - 04:04
Publication date: Available online 3 February 2016 Author(s): Mingxing Tian, Jing Qu, Yanqing Bao, Jianpeng Gao, Jiameng Liu, Shaohui Wang, Yingjie Sun, Chan Ding, Shengqing Yu Brucellosis, the most common widespread zoonotic disease, is caused by Brucella spp., which are facultative, intracellular, Gram-negative bacteria. With the development of molecular biology...
Tue, 01/26/2016 - 02:47
Publication date: Available online 25 January 2016 Author(s): Kurtulus Gokduman, M. Dilek Avsaroglu, Aris Cakiris, Duran Ustek, G. Candan Gurakan The aim of the current study was to develop, a new, rapid, sensitive and quantitative Salmonella detection method using a Real-Time PCR technique based on an inexpensive, easy to produce, convenient and standardized recombinant plasmid...
Sun, 01/17/2016 - 01:33
Publication date: Available online 16 January 2016 Author(s): Hung-Yueh Yeh, Katherine Vargas Serrano, Aimee Silvestry Acosta, Richard J. Buhr Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the...
Wed, 12/02/2015 - 19:14
Publication date: Available online 2 December 2015 Author(s): Margarita Andreadou, Emmanouil Liandris, Maria Gazouli, Antonia Mataragka, Ilias Tachtsidis, Nikolaοs Goutas, Dimitrios Vlachodimitropoulos, John Ikonomopoulos Leishmaniosis is a zoonotic disease that affects millions of people especially in resource-poor setting. The development of reliable diagnostic assays that...
Sat, 11/28/2015 - 19:08
Publication date: December 2015 , Volume 119 Author(s): Jessika Nowak, Cristina D. Cruz, Jon Palmer, Graham C. Fletcher, Steve Flint Listeria monocytogenes 15G01, a strain belonging to the persistent pulsotype 5132, was isolated from a seafood processing plant in New Zealand. Simple monoculture assays using crystal violet staining showed good biofilm formation for this strain and...
Sat, 11/28/2015 - 19:08
Publication date: December 2015 , Volume 119 Author(s): Inés de Cárdenas, José F. Fernández-Garayzábal, María-Luisa de la Cruz, Lucas Domínguez, María Ugarte-Ruiz, Susana Gómez-Barrero This study evaluates an improved scheme for Campylobacter genotyping based on the combination of true and questionable CRISPR (clustered regularly interspaced short palindromic repeats) elements. A...
Mon, 11/23/2015 - 18:57
Publication date: Available online 10 November 2015 Author(s): Teruyo Ojima-Kato, Naomi Yamamoto, Yoshio Iijima, Hiroto Tamura O157, O26, and O111 are the most important O serogroups of enterohemorrhagic Escherichia coli worldwide. Recently we reported a strategy for discriminating these serotypes from the others using matrix-assisted laser desorption ionization-time of flight mass...
Mon, 11/23/2015 - 18:57
Publication date: Available online 10 November 2015 Author(s): Gregoire Seyrig, Robert D. Stedtfeld, Dieter M. Tourlousse, Farhan Ahmad, Keara Towery, Alison M. Cupples, James M. Tiedje, Syed A. Hashsham Loop-mediated isothermal amplification (LAMP) is increasingly used for point-of-care nucleic acid based diagnostics. LAMP can be monitored in real-time by measuring the...
Mon, 11/23/2015 - 18:57
Publication date: Available online 11 November 2015 Author(s): Elodie Sifré, Salha Ben Amor, Astrid Ducournau, Pauline Floch, Hubert Chardon, Francis Mégraud, Philippe Lehours Antimicrobial susceptibility testing of Campylobacter isolates is of great importance for treatment options especially in systemic diseases. The European Committee for Antimicrobial and Susceptibility...
Mon, 11/23/2015 - 18:57
Publication date: Available online 17 November 2015 Author(s): Xin Zhang, Yuxia Li, Bingjuan Li, Yan Mao, Xun Wu, Xiaohua Zou, Peng Gao, Hexin Yan, Dan Yang, Yan Ling, Huipeng Chen Escherichia coli O157:H7 is an enterohemorrhagic E. coli (EHEC) strain and a major food-borne pathogen, causing severe disease in humans worldwide. Multiple sensitive, accurate, and...
Mon, 11/23/2015 - 18:57
Publication date: December 2015 , Volume 119 Author(s): Marialaura Corrente, Costantina Desario, Antonio Parisi, Erika Grandolfo, Domenico Scaltrito, Gesualdo Vesco, Valeriana Colao, Domenico Buonavoglia Bovine brucellosis is diagnosed by official tests, such as Rose Bengal plate test (RBPT) and Complement Fixation test (CFT). Both tests detect antibodies directed against the...
Mon, 11/23/2015 - 18:57
Publication date: December 2015 , Volume 119 Author(s): Gemma A. Vincent, Stephen R. Graves, Jennifer M. Robson, Chelsea Nguyen, Hazizul Hussain-Yusuf, Aminul Islam, Stanley G. Fenwick, John Stenos Worldwide there are few isolate collections of the intracellular bacterium Coxiella burnetii, due to the difficulties associated with working with the organism and the scarcity of...
Fri, 10/09/2015 - 02:32
Publication date: December 2015 , Volume 119 Author(s): Victoria Brusa, Lucía Galli, Luciano H. Linares, Emanuel E. Ortega, Juan P. Lirón, Gerardo A. Leotta Shiga toxin-producing Escherichia coli (STEC) are recognized as food-borne pathogens. We developed and validated two SYBR green PCR (SYBR-PCR) and a real-time multiplex PCR (RT-PCR) to detect stx 1 and stx 2 genes in meat...
Fri, 10/09/2015 - 02:32
Publication date: December 2015 , Volume 119 Author(s): Kentaro Kawatsu, Junko Sakata, Taro Yonekita, Yuko Kumeda We evaluated the utility of an immunochromatographic assay (NH IC TDH) in identifying thermostable direct hemolysin (TDH)-producing Vibrio parahaemolyticus colonies on selective agar plates. The sensitivity of the NH IC TDH assay was 100% (189 samples) and its specificity...
Fri, 09/04/2015 - 01:23
Publication date: Available online 3 September 2015 Author(s): Geik Yong Ang, Choo Yee Yu, Kok Gan Chan, Kirnpal Kaur Banga Singh, Chan Yean Yean In this study, we report for the first time the development of a dry-reagent-based nucleic acid-sensing platform by combining a thermostabilised linear-after-the-exponential (LATE)-PCR assay with a one-step hybridisation-based nucleic...

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