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Journal of Microbiological Methods

Fri, 09/04/2015 - 01:23
Publication date: Available online 3 September 2015 Author(s): Chen Zhang, Peihua Niu, Yanying Hong, Ji Wang, Jingyun Zhang, Xuejun Ma Objectives We aim to develop a multiplex real-time PCR assay to detect the most common pathogens causing community outbreaks of diarrhea. Methods Four reaction systems of fluorescence dye-based real-time PCR assay were performed to amplify genes...
Sun, 08/23/2015 - 01:18
Publication date: Available online 22 August 2015 Author(s): Anja Klančnik, Nataša Toplak, Minka Kovač, Hélène Marquis, Barbara Jeršek The purpose of this study was to develop a PCR-based method for quantification of Listeria monocytogenes adhesion in microtitre plates. We optimized isolation of DNA in the microtitre plates using cell lysis, ultrasound treatment, heating, and...
Fri, 07/24/2015 - 01:30
Publication date: Available online 23 July 2015 Author(s): Byoung-Kwon Hahm, Hyochin Kim, Atul K. Singh, Arun K. Bhunia The bottleneck for accurate detection of foodborne pathogens is separation of target analytes from complex food matrices. Currently used sample preparation methods are cumbersome, arduous and lengthy; thus, a user-friendly system is desirable. A hand-held sample...
Wed, 07/15/2015 - 01:27
Publication date: Available online 14 July 2015 Author(s): Guo Ping Wu , Su Hua Chen , Robert E. Levin A rapid and efficient method for quantification and discrimination of Salmonella. enterica ser . Enteritidis between viable and dead cells killed by heat was developed using ethidium bromide monoazide (EMA) in combination with a real-time loop amplified (Rti-LAMP) DNA assay. The use of 8...
Fri, 07/03/2015 - 01:23
Publication date: Available online 2 July 2015 Author(s): Anders Boysen , Jonas Borch , Thøger Jensen Krogh , Karin Hjernø , Jakob Møller-Jensen Comparative studies of pathogenic bacteria and their non-pathogenic counterparts has led to the discovery of important virulence factors thereby generating insight into mechanisms of pathogenesis. Protein-based antigens for vaccine development...
Fri, 06/19/2015 - 01:27
Publication date: Available online 18 June 2015 Author(s): Junko Sakata , Kentaro Kawatsu , Tadashi Iwasaki , Yuko Kumeda To rapidly and simply determine whether or not bacterial colonies growing on agar were Vibrio parahaemolyticus , we developed an immunochromatographic assay (VP-ICA) using two different monoclonal antibodies (designated mAb-VP34 and mAb-VP109) against the delta...
Fri, 06/19/2015 - 01:27
Publication date: Available online 18 June 2015 Author(s): Loreen R. Stromberg , Zachary R. Stromberg , Afsheen Banisadr , Steven W. Graves , Rodney A. Moxley , Harshini Mukundan Certain Shiga toxin-producing Escherichia coli (STEC) are virulent human pathogens that are most often acquired through contaminated food. The United States Department of Agriculture, Food Safety and...
Fri, 05/22/2015 - 01:19
Publication date: Available online 21 May 2015 Author(s): Wei Hu , Yun Zhang , Hang Yang , Junping Yu , Hongping Wei In this study, a simple and rapid method was developed to transform protein A producing S. aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method is consisting of...
Fri, 05/22/2015 - 01:19
Publication date: Available online 21 May 2015 Author(s): G.N. Na , S.A. Kim , O.C. Kwon , M.S. Rhee The aim of this study was to develop a new selective and differential medium for isolating Shigella sonnei (designated 3SD medium). The new medium was based on three carbohydrates (lactose, sorbitol, and xylose) and a chromogenic substrate (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside...
Wed, 05/20/2015 - 01:22
Publication date: Available online 19 May 2015 Author(s): Bin Wu , Weili Liang , Biao Kan The well-known human pathogenic bacterium, Vibrio cholerae , can enter a physiologically viable but non-culturable (VBNC) state under stress conditions. The differentiation of VBNC cells and nonviable cells is essential for both disease prevention and basic research. Among all the methods for...
Wed, 05/20/2015 - 01:22
Publication date: Available online 19 May 2015 Author(s): Syed Barizuddin , Baskar Balakrishnan , R. Cody Stringer , Majed Dweik A method combining immunomagnetic separation and fluorescent sensing was developed to detect Escherichia coli ( E.coli ) O104:H4. The antibody specific to E. coli O104:H4 were immobilized on protein A-coated magnetic beads. This protein-A-anti E. coli O104:H4...
Mon, 05/04/2015 - 01:22
Publication date: July 2015 , Volume 114 Author(s): Dolapo Lawal , Catherine Burgess , Evonne McCabe , Paul Whyte , Geraldine Duffy Escherichia coli O157 and O26 shedding patterns in cattle are known to vary widely. To address gaps in the understanding of the underlying factors which impact on shedding dynamics, sensitive and rapid quantitative methods which can be applied in...
Mon, 05/04/2015 - 01:22
Publication date: Available online 1 May 2015 Author(s): Mathilde H. Josefsen , Sandra C. Andersen , Julia Christensen , Jeffrey Hoorfar The efficiency of ten widely applied DNA extraction protocols was evaluated for suitability for diagnostic metagenomics. The protocols were selected based on a thorough literature study. Chicken fecal samples inoculated with about 1 × 103 and 1 × 106...
Wed, 04/01/2015 - 01:19
Publication date: Available online 30 March 2015 Author(s): Samuel Gengler , Henri Batoko , Pierre Wattiau An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn 5 derivatives. The method was successfully applied to a gfp -labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization...
Mon, 03/23/2015 - 01:21
Publication date: April 2015 , Volume 111 Author(s): Insook Son , Rachel Binet , Andrew Lin , Thomas S. Hammack , Julie A. Kase To rapidly identify the presence of potentially virulent O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC), a PCR-based Luminex suspension assay was developed to detect the genes coding for four virulence factors ( stx 1, stx 2, eae , and ehxA...
Mon, 03/23/2015 - 01:21
Publication date: Available online 11 March 2015 Author(s): Mathilde H. Josefsen , Arun K. Bhunia , Eva Olsson Engvall , Mette Sofie Rousing Søndergaard , Jeffrey Hoorfar Improved monitoring tools are important for the control of Campylobacter bacteria in poultry production. Standardized reference culture methods issued by national and international standardization organizations are...
Mon, 03/23/2015 - 01:21
Publication date: May 2015 , Volume 112 Author(s): Sven Maurischat , Istvan Szabo , Beatrice Baumann , Burkhard Malorny Salmonella enterica serovar Enteritidis is a major non-typhoid Salmonella serovar causing human salmonellosis mainly associated with the consumption of poultry and products thereof. To reduce infections in poultry, S . Enteritidis live vaccine strains AviPro SALMONELLA...
Thu, 02/05/2015 - 01:14
Publication date: Available online 3 February 2015 Author(s): L. Dieste-Pérez , J.M. Blasco , M.J. de Miguel , I. Moriyón , P.M. Muñoz Swine brucellosis caused by Brucella suis biovar 2 is an emerging disease in Europe. Currently used diagnostic tests for swine brucellosis detect antibodies to the O-polysaccharide (O-PS) of Brucella smooth lipopolysaccharide (S-LPS) but their...
Sun, 01/25/2015 - 01:17
Publication date: Available online 23 January 2015 Author(s): A. Bousquet , S. Henquet , F. Compain , N. Genel , G. Arlet , D. Decré The dissemination of antimicrobial resistance genes in Enterobacteriaceae has been largely attributed to plasmids, circular DNA molecules capable of autonomous replication. Whereas high-copynumber plasmids primarily rely on passive diffusion for plasmid...
Fri, 01/23/2015 - 01:20
Publication date: Available online 20 January 2015 Author(s): Dorien Van den Bossche , Lieselotte Cnops , Jacob Verschueren , Marjan Van Esbroeck Purpose Microscopy is the diagnostic reference standard for the detection of parasites, but it is labor-intensive and requires experience. Rapid diagnostic tests (RDTs) can provide an alternative to microscopy. Methods RDTs from four...
Mon, 01/19/2015 - 01:19
Publication date: Available online 17 January 2015 Author(s): Aditya Narayan Sarangi , Mohtasim Lohani , Rakesh Aggarwal We used a combination of in-silico approaches to identify 168 promising drug targets in the proteome of a multidrug-resistant Listeria monocytogenes strain; of these, one (PBP4) was particularly promising. Virtual screening using it, followed by reverse docking,...
Mon, 01/19/2015 - 01:19
Publication date: Available online 17 January 2015 Author(s): Olivia D. Nigro , Grieg F. Steward Plating environmental samples on vibrio-selective chromogenic media is a commonly used technique that allows one to quickly estimate concentrations of putative vibrio pathogens or to isolate them for further study. Although this approach is convenient, its usefulness depends directly on how...
Sat, 01/17/2015 - 01:17
Publication date: Available online 15 January 2015 Author(s): Deepak B. Rawool , Jess Vergis , Deepthi Vijay , Pankaj Dhaka , Mamta Negi , Manesh Kumar , Amruta Nair , Krupali V. Poharkar , Nitin V. Kurkure , Ashok Kumar , S.V.S. Malik , S.B. Barbuddhe The present study describes the utility of a chromosomal associated fimbrial subunit gene ( fimA ) for screening ‘typical’ as...
Fri, 01/16/2015 - 01:17
Publication date: Available online 14 January 2015 Author(s): Joelle K. Salazar , Yun Wang , Shuijing Yu , Hui Wang , Wei Zhang Serotyping analysis of bacterial pathogens in food products is important for foodborne disease surveillance and outbreak investigations. Traditional immunological techniques are labor-intensive and time-consuming, whereas polymerase chain reaction (PCR)-based...
Sat, 01/10/2015 - 01:14
Publication date: Available online 9 January 2015 Author(s): Hung King Tiong , Steven Hartson , Peter M. Muriana Extracts of surface proteins, with minimal artifacts from contaminating cytosolic components, are highly desirable for investigating surface factors involved in attachment and formation of biofilms by bacteria that are problematic in commercial food processing facilities. In...

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