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Journal of Virological Methods

Tue, 11/06/2018 - 01:13
Available online 3 November 2018 Author(s): Maëlle Bochud, Wolfram Schäfer, Nathan J. Roth, Carlos RosAbstractHepatitis E virus (HEV) is an emerging concern for the safety of plasma-derived medicinal products. The lack of an efficient cell culture system hampers the studies on HEV biology as well as validation studies to test the capacity of virus reduction steps to clear HEV. Hence, a surrogate...
Wed, 10/17/2018 - 01:11
Available online 15 October 2018 Author(s): Melina Elizabeth Barrios, María Dolores Blanco Fernández, Robertina Viviana Cammarata, Carolina Torres, Viviana Andrea MbayedAbstractAlternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters. In this study, F-specific RNA bacteriophages (...
Sat, 08/11/2018 - 01:12
Available online 10 August 2018 Author(s): Niroshan Thanthrige-Don, Oliver Lung, Tara Furukawa-Stoffer, Cody Buchanan, Tomy Joseph, Dale L. Godson, John Gilleard, Trevor Alexander, Aruna AmbagalaAbstractRespiratory and enteric diseases continue to be two major causes of economic losses to the cattle industry worldwide. Despite their multifactorial etiology, the currently available diagnostic...
Wed, 07/11/2018 - 01:13
Available online 21 June 2018 Author(s): Huanan Wang, Feng Cong, Fanwen Zeng, Yuexiao Lian, Xiangnan Liu, Manlin Luo, Pengju Guo, Jingyun MaAbstractA novel swine acute diarrhea syndrome Coronavirus (SADS-CoV) that causes severe diarrhea in suckling piglets was identified in southern China in 2017. A simple and rapid detection test was developed for this virus using real-time RT-LAMP based on the...
Wed, 07/11/2018 - 01:13
Available online 4 July 2018 Author(s): Isabelle da Silva Luz, Marize Pereira MiagostovichAbstractThis study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats. Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (...
Wed, 07/11/2018 - 01:13
Publication date: September 2018 , Volume 259Author(s): Timothy C. Granade, Maja Kodani, Susan K. Wells, Ae.S. Youngpairoj, Silvina Masciotra, Kelly A. Curtis, Saleem Kamili, S. Michele OwenAbstractReal-time PCR assays for nucleic acid testing (NAT) of hepatitis viruses A–E and for HIV-1 and HIV-2 have been developed; however, a multiplex assay that can simultaneously detect all of these agents...
Wed, 07/11/2018 - 01:13
Publication date: September 2018 , Volume 259Author(s): Rim Aouini, Nacira Laamiri, Abdeljelil GhramAbstractBackgroundLow pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken...
Fri, 07/06/2018 - 01:14
Available online 21 June 2018 Author(s): Huanan Wang, Feng Cong, Fanwen Zeng, Yuexiao Lian, Xiangnan Liu, Manlin Luo, Pengju Guo, Jingyun MaAbstractA novel swine acute diarrhea syndrome Coronavirus (SADS-CoV) that causes severe diarrhea in suckling piglets was identified in southern China in 2017. A simple and rapid detection test was developed for this virus using real-time RT-LAMP based on the...
Fri, 07/06/2018 - 01:14
Available online 4 July 2018 Author(s): Isabelle da Silva Luz, Marize Pereira MiagostovichAbstractThis study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats. Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (...
Fri, 07/06/2018 - 01:14
Publication date: September 2018 , Volume 259Author(s): Timothy C. Granade, Maja Kodani, Susan K. Wells, Ae.S. Youngpairoj, Silvina Masciotra, Kelly A. Curtis, Saleem Kamili, S. Michele OwenAbstractReal-time PCR assays for nucleic acid testing (NAT) of hepatitis viruses A–E and for HIV-1 and HIV-2 have been developed; however, a multiplex assay that can simultaneously detect all of these agents...
Fri, 07/06/2018 - 01:14
Publication date: September 2018 , Volume 259Author(s): Rim Aouini, Nacira Laamiri, Abdeljelil GhramAbstractBackgroundLow pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken...
Sat, 06/23/2018 - 02:36
Publication date: Available online 22 June 2018 Author(s): Aouini Rim, Laamiri Nacira, Ghram Abdeljelil Background Low pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated...
Fri, 06/22/2018 - 02:30
Publication date: Available online 21 June 2018 Author(s): Huanan Wang, Feng Cong, Fanwen Zeng, Yuexiao Lian, Xiangnan Liu, Manlin Luo, Pengju Guo, Jingyun Ma A novel swine acute diarrhea syndrome Coronavirus (SADS-CoV) that causes severe diarrhea in suckling piglets was identified in southern China in 2017. A simple and rapid detection test was developed for this virus using...
Mon, 06/04/2018 - 00:43
Publication date: Available online 3 June 2018 Author(s): Timothy C. Granade, Maja Kodani, Susan K. Wells, A.S. Youngpairoj, Silvina Masciotra, Kelly M. Curtis, Saleem Kamili, S. Michele Owen Real-time PCR assays for nucleic acid testing (NAT) of hepatitis viruses A-E and for HIV-1 and HIV-2 have been developed; however, a multiplex assay that can simultaneously detect all of...
Wed, 05/02/2018 - 21:58
Publication date: Available online 2 May 2018 Author(s): Rusheng Zhang, Dong Yao, Jingfang Chen, Wen Ye, Xinhua Ou, Tianmu Chen, Biancheng Sun As of Aug 25, 2017, 17 incidences of human infection and 6 deaths due to the novel H5N6 virus have been reported in China. Genetic analysis of the viral genome revealed that this reassortant virus is highly pathogenic to poultry, and...
Fri, 04/13/2018 - 12:57
Publication date: April 2018 , Volume 254 Author(s): Kyson X. Chou, Donna M. Williams-Hill Rapid advancement in genomics and bioinformatics in recent years holds great promise for research and development in many disciplines including public health. For the detection of pathogens, methods based on nucleic acid amplification need to be re-evaluated periodically to ensure the validity of...
Wed, 12/06/2017 - 06:14
Publication date: February 2018 , Volume 252 Author(s): Viska I. Iskandar, Yutaka Sasaki, Naoto Yoshino, Raden Z.R. Abubakar, Shigehiro Sato, Yasushi Muraki A cell-based vaccine production method for influenza virus may be an effective and more rapid alternative to egg-based systems. For high-yield virus production, the effect of bovine, porcine, fungal, and recombinant trypsins...
Tue, 11/14/2017 - 17:05
Publication date: Available online 10 November 2017 Author(s): Junli Liu, Lu Yao, Feifei Zhai, Yuqing Chen, Jing Lei, Zhenwei Bi, Jianhua Hu, Qian Xiao, Suquan Song, Liping Yan, Jiyong Zhou Avian influenza virus (AIV), especially subtypes H5, H7 and H9, has contributed to enormous economic losses and poses a potential pandemic threat to global human public health. Early...
Tue, 11/14/2017 - 17:05
Publication date: January 2018 , Volume 251 Author(s): Natthanan Sangsriratanakul, Chiharu Toyofuku, Mayuko Suzuki, Miyuki Komura, Masashi Yamada, Md. Shahin Alam, Sakchai Ruenphet, Dany Shoham, Kouji Sakai, Kazuaki Takehara An alkaline agent, namely, food additive grade calcium hydroxide (FdCa(OH)2) in the solution, powder and suspension forms was evaluated as a virucidal...
Tue, 11/14/2017 - 17:05
Publication date: February 2018 , Volume 252 Author(s): Michelle Wille, Hong Yin, Åke Lundkvist, Juan Xu, Shaman Muradrasoli, Josef D. Järhult Surveillance of wild birds is critical in monitoring for highly pathogenic avian influenza A viruses (AIVs). However, a successful surveillance regime requires proper treatment of samples in the field – rapid placement of samples in −80°C...
Sat, 09/30/2017 - 09:42
Publication date: December 2017 , Volume 250 Author(s): Dan Rao, Miaoli Wu, Jing Wang, Wen Yuan, Yujun Zhu, Feng Cong, Fengjiao Xu, Yuexiao Lian, Bihong Huang, Qiwen Wu, Meili Chen, Yu Zhang, Ren Huang, Pengju Guo Murine parvovirus is one of the most prevalent infectious pathogens in mouse colonies. A specific primer pair targeting the VP2 gene of minute virus of...
Thu, 09/28/2017 - 09:09
Publication date: November 2017 , Volume 249 Author(s): Yan Zheng, Yuan Hu Hepatitis A virus (HAV) can cause serious liver disease and even death. HAV outbreaks are associated with the consumption of raw or minimally processed produce, making it a major public health concern. Infections have occurred despite the fact that effective HAV vaccine has been available. Development of a rapid...
Thu, 09/21/2017 - 08:40
Publication date: November 2017 , Volume 249 Author(s): Michael Haryadi Wibowo, Simson Tarigan, Sumarningsih, Sidna Artanto, Risa Indriani, Dito Anggoro, Cahyaditya Pratama Putra, Syafrison Idris, Tri Untari, Widya Asmara, Charles Rangga Tabbu, Jagoda Ignjatovic In countries where highly pathogenic avian influenza virus (HPAIV) H5N1 is endemic and controlled by...
Sat, 09/02/2017 - 08:07
Publication date: November 2017 , Volume 249 Author(s): Liji Xie, Zhixun Xie, Li Huang, Sheng Wang, Jiaoling Huang, Yanfang Zhang, Tingting Zeng, Sisi Luo Sequence analysis of duck plague virus (DPV) revealed that there was a 528bp (B fragment) deletion within the UL2 gene of DPV attenuated vaccine strain in comparison with field virulent strains. The finding of gene deletion...
Tue, 07/25/2017 - 09:55
Publication date: October 2017 , Volume 248 Author(s): Yongjuan Wang, Shanyuan Zhu, Weiming Hong, Anping Wang, Weiyong Zuo In this study, six pairs of specific primers that can amplify DNA fragments of different sizes were designed and synthesized according to viral protein gene sequences published in GenBank. Then, a multiplex PCR method was established for rapid detection of duck...
Sat, 06/24/2017 - 05:41
Publication date: October 2017 , Volume 248 Author(s): Nicole L. Turnage, Kristen E. Gibson Acute gastroenteritis causes the second highest infectious disease burden worldwide. Human enteric viruses have been identified as leading causative agents of acute gastroenteritis as well as foodborne illnesses in the U.S. and are generally transmitted by fecal-oral contamination. There is growing...
Thu, 05/25/2017 - 11:34
Publication date: Available online 25 May 2017 Author(s): Yueyang Yu, Xi Zhang, Baihui Zhao, Ying Sun, Xiaoguan Zhang, Tian Bai, Jian Lu, Zi Li, Liqi Liu, Dayan Wang, Yuelong Shu, Jianfang Zhou, Kun Qin Although exhibiting no or low virulence in poultry, avian influenza virus H7N9 has caused around 1,200 confirmed human infections in China with a case-fatality rate...
Thu, 03/09/2017 - 16:13
Publication date: Available online 9 March 2017 Author(s): Haroldo Cid da Silva, Edimilson Domingos da Silva, Lia Laura Lewis-Ximenez de Souza Rodrigues, Marco Alberto Medeiros Since hepatitis A virus (HAV) production is time-consuming and expensive, the use of recombinant proteins may represent an alternative source of antigens for diagnostic purposes. The present study aimed to...
Sun, 12/11/2016 - 09:32
Publication date: Available online 10 December 2016 Author(s): L. Elvira-González, A.V. Puchades, C. Carpino, A. Alfaro-Fernandez, M.I. Font-San-Ambrosio, L. Rubio, L Galipienso Southern tomato virus (STV) is a double stranded RNA (dsRNA) virus belonging to genus Amalgavirus (family Amalgamaviridae) which has been detected in tomato plants showing stunting, fruit discoloration...
Sat, 10/15/2016 - 11:43
Publication date: Available online 14 October 2016 Author(s): Mitchell K. Monninger, Chrystal A. Nguessan, Candace D. Blancett, Kathleen A. Kuehl, Cynthia A. Rossi, Scott P. Olschner, Priscilla L. Williams, Steven L. Goodman, Mei G. Sun Transmission electron microscopy can be used to observe the ultrastructure of viruses and other microbial pathogens with nanometer...
Wed, 10/12/2016 - 11:30
Publication date: Available online 11 October 2016 Author(s): Kenzo Yonemitsu, Yutaka Terada, Ryusei Kuwata, Dung Nguyen, Nobuyuki Shiranaga, Satomi Tono, Tomoka Matsukane, Mayumi Yokoyama, Kazuo Suzuki, Hiroshi Shimoda, Ai Takano, Masahiko Muto, Ken Maeda Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed...
Thu, 09/15/2016 - 11:28
Publication date: November 2016 , Volume 237 Author(s): Naraporn Sirinonthanawech, Somchaiya Surichan, Aphinya Namsai, Pilaipan Puthavathana, Prasert Auewarakul, Alita Kongchanagul Formulation and quality control of trivalent live-attenuated influenza vaccine requires titration of infectivity of individual strains in the trivalent mix. This is usually performed by selective...
Sat, 09/03/2016 - 06:30
Publication date: November 2016 , Volume 237 Author(s): Sai Mao, XuMin Ou, DeKang Zhu, Shun Chen, GuangPeng Ma, MingShu Wang, RenYong Jia, MaFeng Liu, KunFeng Sun, Qiao Yang, Ying Wu, XiaoYue Chen, AnChun Cheng Duck hepatitis A virus 1 (DHAV-1) is the principal pathogen that causes duck viral hepatitis (DHV), a highly fatal infectious disease in ducklings. Given the...
Fri, 09/02/2016 - 06:21
Publication date: November 2016 , Volume 237 Author(s): Xiaoyu Niu, Hao Chen, Jing Yang, Xianglong Yu, Jinfeng Ti, Aihua Wang, Youxiang Diao The newly emerged disease, duck beak atrophy and dwarfism syndrome (BADS), is caused by novel goose parovirus (N-GPV). Although N-GPV infection has severe consequences, few methods for detecting this virus have been developed. Therefore,...
Mon, 08/08/2016 - 04:51
Publication date: October 2016 , Volume 236 Author(s): Robert M. Lawrence, James D. Zook, Brenda G. Hogue Inherent in the study of viruses is the risk of pathogenic exposure, which necessitates appropriate levels of biosafety containment. Unfortunately, this also limits the availability of useful research instruments that are located at facilities not equipped to handle infectious...
Thu, 08/04/2016 - 04:50
Publication date: October 2016 , Volume 236 Author(s): Zhihui Yang, Susan R. Leonard, Mark K. Mammel, Christopher A. Elkins, Michael Kulka Detection and identification of viruses in food samples are technically challenging due largely to the low viral copy number in contaminated food items, and the lack of effective culture enrichment methods that are amenable to regulatory...
Wed, 08/03/2016 - 04:46
Publication date: October 2016 , Volume 236 Author(s): Qin Hu, Dekang Zhu, Guangpeng Ma, Anchun Cheng, Mingshu Wang, Shun Chen, Renyong Jia, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen Duck hepatitis A virus (DHAV) is a highly infectious pathogen that causes significant bleeding lesions in the viscera of ducklings less than 3 weeks old. There are three...
Fri, 07/29/2016 - 00:41
Publication date: October 2016 , Volume 236 Author(s): Sardar Karash, Ronghui Wang, Lisa Kelso, Huaguang Lu, Tony Jun Huang, Yanbin Li Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance...
Thu, 07/21/2016 - 00:03
Publication date: October 2016 , Volume 236 Author(s): Ana Margarida Henriques, Teresa Fagulha, Sílvia Carla Barros, Fernanda Ramos, Margarida Duarte, Tiago Luís, Miguel Fevereiro Avian influenza (AI) is an infectious viral disease usually asymptomatic in wild birds that can spread to domestic poultry and cause large-scale outbreaks. Some of the AI viruses (AIV) can cross the...
Thu, 07/14/2016 - 05:23
Publication date: October 2016 , Volume 236 Author(s): Jirapat Arunorat, Nataya Charoenvisal, Yonlayong Woonwong, Roongtham Kedkovid, Roongroje Thanawongnuwech Since the introduction of pandemic H1N1 2009 virus (pdmH1N1) in pigs, the status of Thai swine influenza virus (SIV) has changed. The pdmH1N1 and its reassortant viruses have become the predominant strain circulating in the...
Thu, 06/09/2016 - 07:30
Publication date: September 2016 , Volume 235 Author(s): Jiemin Cormier, Marlene Janes Hepatitis A virus (HAV) infection is the leading worldwide cause of acute viral hepatitis, and outbreaks caused by this virus often occur in fecal polluted waters. Rapid concentration and detection of viral contamination in water environments can prevent economic loss and can identify the source of...
Tue, 04/05/2016 - 12:44
Publication date: Available online 4 April 2016 Author(s): Yong Yan, Xiao-jun Jia, Heng-hui Wang, Xiao-fei Fu, Ji-mei Ji, Pei-yan He, Li-xia Chen, Jian-yong Luo, Zhong-wen Chen This study involved a human infection with avian influenza H7N9(A) virus in Zhejiang province, the first one after implementing the closure measures of living poultry markets in China. The clinical...
Mon, 03/28/2016 - 11:49
Publication date: Available online 28 March 2016 Author(s): C. Yu, S. Wales, M.K. Mammel, K. Hida, M. Kulka Viruses are major pathogens causing foodborne illnesses and are often present at low levels in foods, thus requiring sensitive techniques for their detection in contaminated foods. The lack of efficient culture methods for many foodborne viruses and the potential for multi-...
Tue, 03/22/2016 - 11:22
Publication date: Available online 22 March 2016 Author(s): Xihong Zhao, Yu-Chia Tsao, Fu-Jung Lee, Woo-Hu Tsai, Ching-Ho Wang, Tsung-Liang Chuang, Mu-Shiang Wu, Chii-Wann Lin A side-polished fiber optic surface plasmon resonance (SPR) sensor was fabricated to expose the core surface and then deposited with a 40nm thin gold film for the near surface sensing of effective...
Thu, 03/17/2016 - 11:13
Publication date: Available online 16 March 2016 Author(s): Qiudong Su, Minzhuo Guo, Zhiyuan Jia, Feng Qiu, Xuexin Lu, Yan Gao, Qingling Meng, Ruiguang Tian, Shengli Bi, Yao Yi Hepatitis A virus (HAV) infection can stimulate the production of antibodies to structural and non‐structural proteins of the virus. However, vaccination with an inactivated or attenuated HAV...
Thu, 12/24/2015 - 02:44
Publication date: Available online 23 December 2015 Author(s): Jianmei Yang, Xiaoguang Dai, Hongjun Chen, Qiaoyang Teng, Xuesong Li, Guangyu Rong, Liping Yan, Qinfang Liu, Zejun Li A blocking enzyme-linked immunosorbent assay (bELISA) was developed for detection of antibodies against H9N2 avian influenza viruses (AIVs) based on a monoclonal antibody specific to the...
Sat, 11/07/2015 - 09:21
Publication date: January 2016 , Volume 227 Author(s): Dan Zhang, Xiaobo Huang, Xiaohui Zhang, Sanjie Cao, Xintian Wen, Yiping Wen, Rui Wu, Entao Liang This research aimed to develop an oral vaccine for transmissible gastroenteritis virus (TGEV) based on the TGEV N gene expressed in an attenuated Salmonella typhimurium vector and aimed to evaluate the vaccine's immune response...
Sat, 09/26/2015 - 01:20
Publication date: Available online 25 September 2015 Author(s): Ute Wessels, Olga Stech, El-Sayed M. Abdelwhab, Andreas Judel, Thomas C. Mettenleiter, Jürgen Stech Reverse genetics of influenza A viruses facilitates both basic research and vaccine development. However, efficient cloning of virus gene segments was cumbersome in established systems due to the necessary cleavage of...
Fri, 09/11/2015 - 06:20
Publication date: 1 December 2015 , Volume 225 Author(s): Youlin Shen, Anchun Cheng, Mingshu Wang, Shun Chen, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao Yang, Xiaoyue Chen An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the recombinant VP3 protein of duck hepatitis A virus type 1 (DHAV-1) was developed and evaluated in this study. The optimal...
Thu, 09/10/2015 - 06:20
Publication date: November 2015 , Volume 224 Author(s): A. Perrin, J. Loutreul, N. Boudaud, I. Bertrand, C. Gantzer In recent years, foodborne viruses, especially human noroviruses (NoV) and hepatitis A virus (HAV), have been increasingly reported as the causes of foodborne disease outbreaks. Soft red fruits, especially raspberries, have a high incidence among the types of food...

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