As patulin is a kind of mycotoxin commonly found in fruit products, it is of great importance to develop simple and accurate analytical methods for the sensitive detection of patulin (PAT). In this work, based on molecular imprinted polymer (MIP) and surface-enhanced Raman scattering (SERS) technique, a specific and sensitive PAT sensor was developed. To prepare the MIP-SERS...
Peanuts with fungal contamination may contain aflatoxin, a highly carcinogenic substance. We propose the use of hyperspectral imaging to quickly and noninvasively identify fungi-contaminated peanuts. The spectral data and spatial information of hyperspectral images were exploited to improve identification accuracy. In addition, successive projection was adopted to...
Toxins, Vol. 11, Pages 378: Pig Urinary Concentration of Mycotoxins and Metabolites Reflects Regional Differences, Mycotoxin Intake and Feed Contaminations
Toxins doi: 10.3390/toxins11070378
The determination of mycotoxin and metabolite concentrations in human and animal urine is currently used for risk assessment and mycotoxin...
Toxins, Vol. 11, Pages 377: Ochratoxin A-Induced Hepatotoxicity through Phase I and Phase II Reactions Regulated by AhR in Liver Cells
Toxins doi: 10.3390/toxins11070377
Hye Soo Shin
Hyun Jung Lee
Min Cheol Pyo
Ochratoxin A (OTA) is a widespread mycotoxin produced by several species of the genera Aspergillus and Penicillium. OTA exists in a variety...
Publication date: Available online 25 June 2019Source: Author(s): Jingyuan Yao, Wei Jin, Dongmei Li, Daoyan Xu, Shiyong Wen, Renyan Liu, Yubo Liang, Songhui LuAbstractParalytic shellfish toxins (PSTs) are a group of the most harmful neurotoxins distributed worldwide. Marine organism samples, including mollusks, crustaceans and fish, collected from 11 sites around the coastal water of the South...
Toxins, Vol. 11, Pages 375: Global Mycotoxin Occurrence in Feed: A Ten-Year Survey
Toxins doi: 10.3390/toxins11070375
Mycotoxins contaminating animal feed can exert toxic effects in animals and be transferred into animal products. Therefore, mycotoxin occurrence in feed should be monitored. To this end, we performed...
Publication date: September 2019 Food and Chemical Toxicology, Volume 131Author(s): Hans Steinkellner, Marco Binaglia, Chiara Dall'Asta, Arno C. Gutleb, Manfred Metzler, Isabelle P. Oswald, Dominique Parent-Massin, Jan AlexanderAbstractThis paper describes a methodology for hazard assessment of groups of related substances for which toxicity data are insufficient, and which utilises, next to...
Controlling aflatoxigenic Aspergillus flavus (Af) and aflatoxins in grains and food during storage are great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of Af in un-contacted face-to-face dual culture tests. Gas chromatography tandem mass spectrometry revealed that dimethyl...
Aflatoxin B1 (AfB1) is a carcinogenic mycotoxin that contaminates food and feed worldwide. We determined the AfB1-adsorption capability of non-viable Pleurotus eryngii mycelium, an edible fungus, as a potential means for removal of AfB1 from contaminated solutions. Lyophilized mycelium was produced and made enzymatically inert by sterilization at high temperatures . The material thus obtained...
The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and commercialized in State of Parana, Brazil. The extraction method carried out with a Romer Mycosep 226 column was found to be efficient due to addition of both diatomaceous earth and centrifuging during the cleaning step. From the total of 84 analyzed samples, 78.6% (n = 66) were found to be...
Journal of Food Protection, Volume 82, Issue 7, Page 1176-1182, July 2019.
Publication date: 15 November 2019 , Volume 298Author(s): Jinghua Wu, Lingwen Zeng, Nianlong Li, Chengshuai Liu, Junhua ChenAbstractA wash-free and label-free colorimetric biosensor for the amplified detection of aflatoxin B1 (AFB1) has been constructed by the integration of an ingenious hairpin DNA probe with exonuclease III (Exo III)-assisted signal amplification. The presence of the AFB1...
Available online 18 June 2019 Author(s): An-Dong Gong, Fei-Yan Dong, Meng-Jun Hu, Xian-Wei Kong, Fen-Fen Wei, Shuang-Jun Gong, Yi-Mei Zhang, Jing-Bo Zhang, Ai-Bo Wu, Yu-Cai LiaoAbstractThe contamination of food with Aspergillus flavus and subsequent aflatoxins is considered as one of the most severe safety problems in the world. The application of microorganisms and the produced bio-active...
Available online 18 June 2019 Author(s): Jomana Elaridi, Hani Dimassi, Hussein HassanAbstractAflatoxin M1 (AFM1) and ochratoxin A (OTA) are hepatotoxin and nephrotoxin mycotoxins, respectively. An investigation on their presence in baby formulae marketed in Lebanon was conducted. Infant formula samples were collected from 42 brands over two production dates (n = 84) during 2017–2018 and analyzed...
Publication date: Available online 7 June 2019Source: LWTAuthor(s): Sofía Sampaolesi, Raúl Ricardo Gamba, Graciela Liliana De Antoni, Ángela María León PeláezAbstractBeer is the most consumed alcoholic beverage worldwide and brewery is a growing industry. Biomass by-product of beer production is constituted by viable and non-viable flocculated yeasts which are discarded. To increase the value of...
This study aimed to evaluate the ability of the Lactobacillus rhamnosus strain LC‐4 to bind aflatoxin M1 (AFM1) in phosphate‐buffered saline (PBS) and yogurt. Bacterial cells were subjected to heat, acid, and alkali treatments. The AFM1‐binding rate of acid‐treated bacteria was of 78.63 ± 0.52%. The binding of L. rhamnosus LC‐4 to AFM1 was partially reversible and the binding of AFM1...
Publication date: Available online 17 June 2019 Food and Chemical ToxicologyAuthor(s): Qin Zhao, Zhao-Si Yang, San-Jie Cao, Yung-Fu Chang, Yu-Qin Cao, Jia-Bing Li, Zi-Xuan Yao, Yi-Ping Wen, Xiao-Bo Huang, Rui Wu, Qi-Gui Yan, Yong Huang, Xiao-Ping Ma, Xin-Feng Han, Yinglong WuAbstractCadmium and aflatoxin B1 (AFB1) are both common and widespread pollutants in food and feed. There are several...
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.9b02595
The detoxification ability of Lactobacillus acidophilus and prebiotics was evaluated by a Plackett‐Burman Design to examine the reduction of concentration and bioaccessibility of aflatoxin B1 (AFB1) and M1 (AFM1) in artificially contaminated whole cow's milk. Six variables were evaluated: AFB1 (3.25–6.0 μg L−1) or AFM1 (1.0–2.0 μg L−1) concentration; incubation time (0–6 h); and inulin,...
Available online 14 June 2019 Author(s): Takashi Yarita, Shinsuke Inagaki, Ayano Miyamoto, Taichi Yamazaki, Migaku Kawaguchi, Hajime Uchida, Akiko Takatsu, Toshiyuki SuzukiAbstractA scallop midgut gland certified reference material, NMIJ CRM 7520-a, was developed for validation and quality assurance during the inspection of shellfish for diarrhetic shellfish toxins. The candidate material was...
Toxins, Vol. 11, Pages 342: Occurrence of Mycotoxins in Swine Feeding from Spain
Toxins doi: 10.3390/toxins11060342
Ana M. García-Campaña
A survey including 228 pig feed samples from Spain has been developed, exploring the occurrence of 19 mycotoxins (aflatoxins B1, B2, G1 and...
Publication date: September 2019Source: LWT, Volume 112Author(s): Tiago de Melo Nazareth, Juan Manuel Quiles, Raquel Torrijos, Fernando Bittencourt Luciano, Jordi Mañes, Giuseppe MecaAbstractThe present study evaluated the efficacy of allyl isothiocyanate (AITC) in avoiding the growth of Penicillium verrucosum and consequent ochratoxin A (OTA) production in barley during storage for 90 d....
Toxins, Vol. 11, Pages 341: Cytotoxicity, Genotoxicity and Disturbance of Cell Cycle in HepG2 Cells Exposed to OTA and BEA: Single and Combined Actions
Toxins doi: 10.3390/toxins11060341
Mycotoxins are produced by a number of fungal genera spp., for example, Aspergillus, Penicillium, Alternaria, Fusarium, and Claviceps. Beauvericin (BEA) and...
Toxins, Vol. 11, Pages 337: Cold Plasma Treatment as an Alternative for Ochratoxin a Detoxification and Inhibition of Mycotoxigenic Fungi in Roasted Coffee
Toxins doi: 10.3390/toxins11060337
Paloma Patricia Casas-Junco
Josué Raymundo Solís-Pacheco
Juan Arturo Ragazzo-Sánchez
Blanca Rosa Aguilar-Uscanga
Pedro Ulises Bautista-Rosales
Toxins, Vol. 11, Pages 333: Effects of Dietary Zearalenone on Oxidative Stress, Cell Apoptosis, and Tight Junction in the Intestine of Juvenile Grass Carp (Ctenopharyngodon idella)
Toxins doi: 10.3390/toxins11060333
Zearalenone (ZEA) is a prevalent...
Toxins, Vol. 11, Pages 331: Occurrence of Tetrodotoxin in Bivalves and Gastropods from Harvesting Areas and Other Natural Spaces in Spain
Toxins doi: 10.3390/toxins11060331
Ana G. Cabado
Tetrodotoxin (TTX) is a potent neurotoxin that is receiving increasing interest in the European Union because...
Available online 4 June 2019 Author(s): Suyan Qiu, Lijuan Yuan, Yihua Wei, Dawen Zhang, Qinglong Chen, Zhenyu Lin, Linguang LuoAbstractA novel signal-on portable sensing system has been developed for OTA detection using personal glucose meter (PGM) as signal transducer. In the study, we explore the potential of using a short dsDNA as template to trigger the “click” ligation of two DNA strands,...
Toxins, Vol. 11, Pages 329: Occurrence of the Ochratoxin A Degradation Product 2′R-Ochratoxin A in Coffee and Other Food: An Update
Toxins doi: 10.3390/toxins11060329
Food raw materials can contain the mycotoxin ochratoxin A (OTA). Thermal processing of these materials may result in...
Toxins, Vol. 11, Pages 330: Application of Zearalenone (ZEN)-Detoxifying Bacillus in Animal Feed Decontamination through Fermentation
Toxins doi: 10.3390/toxins11060330
Zearalenone (ZEN) is an estrogenic mycotoxin which can cause loss in animal production....
Toxins, Vol. 11, Pages 328: Fumonisins: Impact on Agriculture, Food, and Human Health and their Management Strategies
Toxins doi: 10.3390/toxins11060328
Dipendra K. Mahato
Kyung Eun Lee
Sang G. Kang
The fumonisins producing fungi, Fusarium spp., are ubiquitous in nature and contaminate several food matrices that pose detrimental...
Publication date: Available online 5 June 2019 Food and Chemical ToxicologyAuthor(s): Tongtong Shen, Yufan Miao, Chenchen Ding, Wentao Fan, Shuhui Liu, Yanan Lv, Xiaona Gao, Marthe De Boevre, Liping Yan, Sheila Okoth, Sarah De Saeger, Suquan SongAbstractZearalenone (ZEA) can widely contaminate crops and agricultural products. The ingestion of ZEA-contaminated food or feed affects the integrity...
Available online 3 June 2019 Author(s): Josué Delgado, Félix Núñez, Miguel A. Asensio, Rebecca A. OwensAbstractDry-cured meat products are usually contaminated with moulds during ripening. Although fungal development contributes to the desired sensory characteristics, some moulds, such as Penicillium nordicum are able to produce ochratoxin A (OTA) on meat products. Therefore, strategies to...
Application of atoxigenic strains to compete against toxigenic strains of Aspergillus flavus strains has emerged as one of the practical strategies for reducing aflatoxin contamination in corn, peanut, and tree nuts. The actual mechanism that results in aflatoxin reduction is not fully understood. Real-time RT-PCR and relative quantification of gene expression protocol...
Toxins, Vol. 11, Pages 315: Mitigating Aflatoxin Contamination in Groundnut through A Combination of Genetic Resistance and Post-Harvest Management Practices
Toxins doi: 10.3390/toxins11060315
Manish K. Pandey
Arun K. Pandey
Sunil S. Gangurde
Hari K. Sudini
Jake C. Fountain
Publication date: Available online 30 May 2019 Food and Chemical ToxicologyAuthor(s): Jochem Louisse, Deborah Rijkers, Geert Stoopen, Wendy Jansen Holleboom, Mona Delagrange, Elise Molthof, Patrick P.J. Mulder, Ron L.A.P. Hoogenboom, Marc Audebert, Ad A.C.M. PeijnenburgAbstractPyrrolizidine alkaloids (PAs) are secondary metabolites from plants that have been found in substantial amounts in herbal...
Recent studies from our laboratory indicate that engineered silver nanoparticles can inhibit aflatoxin biosynthesis even at concentrations at which they do not demonstrate antifungal activities on the aflatoxin-producing fungus. Whether such inhibition can be modified by altering the nanoparticles’ physical properties remains unclear. In this study, we demonstrate...
Available online 28 May 2019 Author(s): Xianfeng Ren, Qi Zhang, Wenhua Wu, Tingting Yan, Xiaoqian Tang, Wen Zhang, Li Yu, Peiwu LiAbstractAn anti-idiotypic nanobody-phage display-mediated immuno-polymerase chain reaction (PD-IPCR) method was developed for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals. Two phages, displaying the variable domain of the heavy...
Available online 29 May 2019 Author(s): Ling Guo, Yanyan Wang, Peng Fei, Jianxin Liu, Daxi RenABSTRACTThe objective of this study was to investigate the levels of aflatoxin M1 (AFM1) concentration in raw milk and dairy products from water buffalo in south China. 136 raw milk and 86 dairy products samples were collected randomly from buffalo dairy pastures and supermarket in Guangxi, Guizhou,...
Toxins, Vol. 11, Pages 312: Mycotoxins at the Start of the Food Chain in Costa Rica: Analysis of Six Fusarium Toxins and Ochratoxin A between 2013 and 2017 in Animal Feed and Aflatoxin M1 in Dairy Products
Toxins doi: 10.3390/toxins11060312
Mycotoxins are secondary...
Toxins, Vol. 11, Pages 309: Separation and Purification of Aflatoxins by Centrifugal Partition Chromatography
Toxins doi: 10.3390/toxins11060309
Aflatoxins are mycotoxins that are produced by several species of filamentous fungi. In the European Union, the concentration limits...
Toxins, Vol. 11, Pages 308: Occurrence of Deoxynivalenol and Ochratoxin A in Beers and Wines Commercialized in Paraguay
Toxins doi: 10.3390/toxins11060308
Andrea Alejandra Arrúa
Juliana Moura Mendes
Francisco Paulo Ferreira
Man Mohan Kohli
Danilo Fernández Ríos
Alcoholic beverages can...
Toxins, Vol. 11, Pages 306: Tetrodotoxins Occurrence in Non-Traditional Vectors of the North Atlantic Waters (Portuguese Maritime Territory, and Morocco Coast)
Toxins doi: 10.3390/toxins11060306
Ana Isabel Neto
Luis M. Botana
Toxins, Vol. 11, Pages 305: Acute Toxicity Assessment: Macroscopic and Ultrastructural Effects in Mice Treated with Oral Tetrodotoxin
Toxins doi: 10.3390/toxins11060305
M. Carmen Louzao
Mercedes R. Vieytes
Luis M. Botana
Tetrodotoxin (TTX) is an extremely toxic marine compound produced by different genera of bacteria that can reach humans...
Toxins, Vol. 11, Pages 304: Assorted Methods for Decontamination of Aflatoxin M1 in Milk Using Microbial Adsorbents
Toxins doi: 10.3390/toxins11060304
Jean Claude Assaf
André El Khoury
Aflatoxins (AF) are carcinogenic metabolites produced by different species of Aspergillus which readily colonize crops. AFM1 is secreted in...
Toxins, Vol. 11, Pages 301: Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme
Toxins doi: 10.3390/toxins11050301
Md Shofiul Azam
Zearalenone (ZEA) is an estrogenic and ochratoxin A (OTA) is a hepatotoxic Fusarium mycotoxin commonly seen in cereals and fruits products. No previous investigation has studied...
Publication date: Available online 24 May 2019 Food and Chemical ToxicologyAuthor(s): Nurshad Ali, Gisela H. DegenAbstractThe Fusarium toxin zearalenone (ZEN) is of concern due to its pronounced estrogenic effects in mammalian species. ZEN contaminates various cereal crops and grain-based food along with modified forms which contribute to overall mycoestrogen exposure. As no data exist on the...
Publication date: 15 October 2019 , Volume 295Author(s): Maria Carla CraveroAbstractThis review collates the scientific literature regarding organic and biodynamic wines. It concerns techniques that allow organic or biodynamic wines to be distinguished from conventional products. Other aspects of the organic wines addressed include the antioxidant capacities and the content of compounds...
Publication date: Available online 23 May 2019 Food and Chemical ToxicologyAuthor(s): Cathy Lester, John Troutman, Cindy Obringer, Ken Wehmeyer, Peter Stoffolano, Michael Karb, Yan Xu, Amy Roe, Greg Carr, Karen Blackburn, Catherine MahonyAbstract1,2-Unsaturated pyrrolizidine alkaloids (PAs) are sometimes present in foods or herbal supplements/medicines as impurities and pose potential concerns...
Toxins, Vol. 11, Pages 296: Time-Dependent Changes in the Intestinal Microbiome of Gilts Exposed to Low Zearalenone Doses
Toxins doi: 10.3390/toxins11050296
Maciej T. Gajęcki
Zearalenone is a frequent contaminant of cereals and their by...