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Cryptosporidium parvum

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Sun, 03/17/2019 - 01:12
Available online 16 March 2019 Author(s): Panagiota Ligda, Edwin Claerebout, Lucy J. Robertson, Smaragda SotirakiAbstractMarine bivalve shellfish are of public health interest because they can accumulate pollutants in their tissues. As they are usually consumed raw or lightly cooked, they are considered to be a possible source of foodborne infections, including giardiosis and cryptosporidiosis....
Wed, 02/06/2019 - 01:08
Publication date: August 2018Source: Microbial Risk Analysis, Volume 9Author(s): Mary E. Schoen, Michael A. Jahne, Jay GarlandAbstractWe assessed the annual probability of infection resulting from non-potable exposures to distributed greywater and domestic wastewater treated by an aerobic membrane bioreactor (MBR) followed by chlorination. A probabilistic quantitative microbial risk assessment...
Fri, 08/31/2018 - 11:00
In this study, we assessed the prevalence and genetic characteristics of Cryptosporidium in sheep from 10 provinces in China. Fecal samples from 1,035 sheep originating from 16 farms were collected, and 295 (28.5%) were found to be Cryptosporidium positive by nested PCR. Cryptosporidium was detected at all farms, with infection rates between 5.7% and 50.0%. Three Cryptosporidium species were...
Sat, 08/11/2018 - 01:12
Available online 10 August 2018 Author(s): Niroshan Thanthrige-Don, Oliver Lung, Tara Furukawa-Stoffer, Cody Buchanan, Tomy Joseph, Dale L. Godson, John Gilleard, Trevor Alexander, Aruna AmbagalaAbstractRespiratory and enteric diseases continue to be two major causes of economic losses to the cattle industry worldwide. Despite their multifactorial etiology, the currently available diagnostic...
Fri, 07/06/2018 - 01:14
Publication date: Available online 1 February 2018Source: Microbial Risk AnalysisAuthor(s): Mary E. Schoen, Michael A. Jahne, Jay GarlandAbstractWe assessed the annual probability of infection resulting from non-potable exposures to distributed greywater and domestic wastewater treated by an aerobic membrane bioreactor (MBR) followed by chlorination. A probabilistic quantitative microbial risk...
Fri, 07/06/2018 - 01:13
Publication date: November 2018 , Volume 93Author(s): Radka Dziedzinska, Monika Moravkova, Jakub Hrdy, Iva Slana, Hana Vlkova, Hana Kunstovna, Petra VasickovaAbstractFresh squeezed juices and smoothies currently enjoy great popularity among consumers. However, data regarding the microbiological safety of these products sold to the public in food outlets are either very limited or missing...
Thu, 05/17/2018 - 07:46
Publication date: Available online 16 May 2018 Author(s): S.M. Raabis, T.L. Ollivett, M.E. Cook, J.M. Sand, S.M. McGuirk The primary objective of this randomized controlled trial was to determine whether anti-IL-10 egg yolk antibodies fed upon arrival to a calf ranch would lower the prevalence of Cryptosporidium parvum shedding in naturally challenged preweaned dairy calves. The...
Thu, 05/03/2018 - 10:24
Publication date: Available online 2 May 2018 Author(s): Adriana Calderaro, Monica Martinelli, Mirko Buttrini, Sara Montecchini, Silvia Covan, Sabina Rossi, Francesca Ferraglia, Paolo Montagna, Federica Pinardi, Sandra Larini, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto This study represents a 2-year picture of the epidemiology...
Sat, 11/25/2017 - 06:22
Publication date: December 2017 , Volume 100, Issue 12 Author(s): Kathryn J. Boor, Martin Wiedmann, Sarah Murphy, Sam Alcaine Microbes that may be present in milk can include pathogens, spoilage organisms, organisms that may be conditionally beneficial (e.g., lactic acid bacteria), and those that have not been linked to either beneficial or detrimental effects on product quality or...
Fri, 10/06/2017 - 15:27
Publication date: 18 December 2017 , Volume 263 Author(s): Kjersti Selstad Utaaker, Anil Kumar, Himanshu Joshi, Suman Chaudhary, Lucy J. Robertson Fresh produce has been recognized as a vehicle of infection for protozoan parasites, particularly Cryptosporidium, and, to a lesser extent, Giardia. For both parasites, outbreaks associated with fresh produce have been documented....
Sun, 08/20/2017 - 07:30
Abstract AimsTo assess diarrheal risks from enteropathogenic E. coli, Giardia, and Cryptosporidium from consuming raw spinach, cabbage, carrots, and tomatoes in Kathmandu Valley, Nepal. Methods and ResultsThe annual infection risk was quantified using the probabilistic Quantitative Microbial Risk Assessment approach, which considered 12 vegetable washing combinations. A new model was used to...
Tue, 06/13/2017 - 19:49
Publication date: October 2017 , Volume 67 Author(s): Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in ‘...
Sat, 06/03/2017 - 09:24
Publication date: 16 August 2017 , Volume 255 Author(s): Kjersti Selstad Utaaker, Eystein Skjerve, Lucy J. Robertson Fresh produce has been recognized as a vehicle for transmission of protozoan parasites for many years, and there are numerous publications regarding their occurrence on such foodstuffs, indicating their potential importance as foodborne parasites. Nevertheless, few...
Tue, 03/07/2017 - 17:00
Mario Santoro, Nicola D’Alessio, Anna Cerrone, Maria Gabriella Lucibelli, Giorgia Borriello, Gaetano Aloise, Clementina Auriemma, Nunzia Riccone, Giorgio Galiero Canine monocytic ehrlichiosis and rickettsiosis are zoonotic tick-borne diseases of canids caused the intracellular obligate bacteria Ehrlichia canis and Rickettsia species respectively. In this study, we investigated using standard and...
Thu, 03/02/2017 - 21:53
Publication date: Available online 2 March 2017 Source:Microbial Risk Analysis Author(s): Mary E. Schoen, Nicholas J. Ashbolt, Michael A. Jahne, Jay Garland This paper presents risk-based enteric pathogen log reduction targets for non-potable and potable uses of a variety of alternative source waters (i.e., locally-collected greywater, roof runoff, and stormwater). A probabilistic...
Mon, 01/16/2017 - 17:19
Publication date: Available online 16 January 2017 Author(s): Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones In this study, the use of skimmed milk flocculation (SMF) to...
Fri, 01/06/2017 - 13:32
Publication date: Available online 9 November 2016 Source:Microbial Risk Analysis Author(s): Michael A. Jahne, Mary E. Schoen, Jay L. Garland, Nicholas J. Ashbolt As decentralized water reuse continues to gain popularity, risk-based treatment guidance is increasingly sought for the protection of public health. However, efforts to evaluate pathogen risks and log-reduction requirements...
Fri, 01/06/2017 - 13:32
Publication date: December 2016 Source:Microbial Risk Analysis, Volume 4 Author(s): Samuel Fuhrimann, Mirko S. Winkler, Michelle Stalder, Charles B. Niwagaba, Mohammed Babu, Narcis B. Kabatereine, Abdullah A. Halage, Jürg Utzinger, Guéladio Cissé, Maarten Nauta In wastewater systems in Kampala, Uganda, microbial contamination has increased over the past two decades. Those...
Mon, 12/05/2016 - 03:18
Cryptosporidium is recognized as one of the main causes of childhood diarrhea worldwide. However, the current treatment for cryptosporidiosis is suboptimal. Calcium flux is essential for entry in apicomplexan parasites. Calcium-dependent protein kinases (CDPKs) are distinct from protein kinases of mammals, and the CDPK1 of the apicomplexan Cryptosporidium lack side chains that typically block a...
Fri, 11/18/2016 - 17:00
Eun Jeong Won, Soo Hyun Kim, Seung Jung Kee, Jong Hee Shin, Soon Pal Suh, Jong Yil Chai, Dong Wook Ryang, Myung Geun Shin Intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. We developed a multiplex real-time PCR assay for detection of eight intestinal parasites and...
Thu, 11/03/2016 - 06:39
Publication date: Available online 3 November 2016 Author(s): C.G. Todd, K.E. Leslie, S.T. Millman, V. Bielmann, N.G. Anderson, J.M. Sargeant, T.J. DeVries The objectives of this study were to evaluate the effects of free-access acidified milk replacer feeding on the pre- and postweaning health of dairy and veal calves. Individually housed calves were systematically assigned at...
Thu, 06/30/2016 - 11:01
Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): M. Jimenez, H. Bridle We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also...
Mon, 05/02/2016 - 17:00
Teklu Wegayehu, Robiul Karim, Manyazewal Anberber, Haileeyesus Adamu, Berhanu Erko, Longxian Zhang, Getachew Tilahun The burden of cryptosporidiosis due to Cryptosporidium parvum is well documented in HIV-positive patients in Ethiopia. However, the role of animals in zoonotic transmission of the disease is poorly understood. The aim of this study was to determine the prevalence and genotypes of...
Fri, 04/08/2016 - 20:48
Publication date: August 2016 , Volume 57 Author(s): Jeanne Hohweyer, Catherine Cazeaux, Emmanuelle Travaillé, Emilie Languet, Aurélien Dumètre, Dominique Aubert, Christine Terryn, Jitender P. Dubey, Nadine Azas, Maryline Houssin, Favennec Loïc, Isabelle Villena, Stéphanie La Carbona Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging...
Fri, 03/25/2016 - 11:01
Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a...
Wed, 11/04/2015 - 09:16
Abstract AimsThis study investigated waterborne opportunistic pathogens (OPs) including potential hosts, and evaluated the use of Legionella spp. for indicating microbial water quality for OPs within a full-scale operating drinking water distribution system (DWDS). Methods and ResultsTo investigate the occurrence of specific microbial pathogens within a major city DWDS we examined large volume (...
Mon, 09/28/2015 - 03:29
Canada geese (Branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. Further, increases in both the Canada geese populations and their ideal habitat requirements in the United States (US) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. Combining these...
Sat, 08/08/2015 - 01:19
Diarrhea causes significant morbidity and mortality in Indian children under 5 years of age. Flies carry enteric pathogens and may mediate foodborne infections. In this study, we characterized fly densities as a determinant of infectious diarrhea in a longitudinal cohort of 160 urban and 80 rural households with 1,274 individuals (27% under 5 years of age) in Vellore, India. Household...
Fri, 07/17/2015 - 01:19
Publication date: January 2016 , Volume 59 Author(s): Emmanuelle Travaillé , Stéphanie La Carbona , Gilles Gargala , Dominique Aubert , Karine Guyot , Aurélien Dumètre , Isabelle Villena , Maryline Houssin This study evaluates the viability of Giardia intestinalis cysts , Cryptosporidium spp. and Toxoplasma gondii oocysts, which may be found in water and food matrices subjected to...
Thu, 05/28/2015 - 01:27
Caoimhe McKerr, Goutam K. Adak, Gordon Nichols, Russell Gorton, Rachel M. Chalmers, George Kafatos, Paul Cosford, Andre Charlett, Mark Reacher, Kevin G. Pollock, Claire L. Alexander, Stephen Morton Background We report a widespread foodborne outbreak of Cryptosporidium parvum in England and Scotland in May 2012. Cases were more common in female adults, and had no history of foreign travel. Over...
Sat, 05/09/2015 - 03:20
Publication date: Available online 9 May 2015 Author(s): Kjersti Selstad Utaaker , Qirong Huang , Lucy J. Robertson Fresh produce is a recognized vehicle for transmission of various protozoan parasites, including Toxoplasma gondii , Cyclospora caytenanensis , Giardia duodenalis , and Cryptosporidium spp. For Giardia and Cryptosporidium , a Standard ISO Method for analyzing fresh produce...
Sat, 04/18/2015 - 01:21
Aim To examine the occurrence and quantity of potential pathogens and an indicator of microbial contamination in the sediments of municipal drinking water storage tanks (MDWSTs), given the absence of such data across the United States. Methods and Results Sediment samples (87 MDWST) from eighteen locations across ten states of the U.S. were collected and assayed by qPCR for a range of potential...
Fri, 01/30/2015 - 00:08
Publication date: March 2015 , Volume 110 Author(s): Dorien Van den Bossche , Lieselotte Cnops , Jacob Verschueren , Marjan Van Esbroeck Purpose Microscopy is the diagnostic reference standard for the detection of parasites, but it is labor-intensive and requires experience. Rapid diagnostic tests (RDTs) can provide an alternative to microscopy. Methods RDTs from four different...
Tue, 01/20/2015 - 14:53
Paediatric acute gastroenteritis is a global public health problem.Comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections.Our study aimed to investigate the role of various pathogens in infantile diarrhoea in Bulgaria...