Cyclospora cayetanensis

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Mon, 04/05/2021 - 06:09
Author(s): Sonia Almeria, Alicia Shipley Recently, outbreaks of Cyclospora cayetanensis in the U.S. were linked to the consumption of a variety of salads containing romaine and/or iceberg lettuce, carrots and/or red cabbage. The Bacteriological Analytical Manual (BAM) Chapter 19 b method was validated for the detection of C. cayetanensis in carrots, cabbage and romaine lettuce, but has not been...
Sat, 03/20/2021 - 06:14
Author(s): Alessandra Barlaam, Tamirat T. Temesgen, Kristoffer R. Tysnes, Laura Rinaldi, Nicola Ferrari, Anna R. Sannella, Giovanni Normanno To investigate the presence of Cyclospora cayetanensis, Toxoplasma gondii and Echinococcus spp. in fresh produce sold in Italy, 324 locally produced ‘ready-to-eat’ (RTE) mixed salad packages belonging to three brands and 324 berries packages (blueberries and...
Thu, 12/24/2020 - 06:16
Author(s): S. Almeria, Angela Assurian, Alicia Shipley Although multiple outbreak clusters of Cyclospora cayetanensis have been traced back to consumption of dishes in Mexican-style restaurants, the FDA Bacteriological Analytical Manual (BAM) does not currently provide methods to detect C. cayetanensis in dishes that contain multiple produce ingredients, such as salsas and guacamole. These...
Wed, 11/11/2020 - 10:02
Cyclospora cayetanensis is a protozoan parasite that causes foodborne and waterborne diarrheal illness outbreaks worldwide. Most of these outbreaks are associated with the consumption of fresh produce. Sensitive and specific methods to detect C. cayetanensis in agricultural water are needed to identify the parasite in agricultural water used to irrigate crops that have been implicated in...
Wed, 06/10/2020 - 04:03
Inhibited reactions have been occasionally observed when cilantro samples were processed for the detection of Cyclospora cayetanensis using real time PCR (qPCR). Partial or total inhibition of PCR reactions, including qPCR, can occur leading to decreased sensitivity or false negative results. If inhibition occurs, this implies the need for additional purification or clean-up treatments of the...
Tue, 05/19/2020 - 03:49
Berries are potential vehicles for the transmission of parasites and have been implicated in illness outbreaks in various countries around the world, particularly in the USA. Although data on contamination of fresh produce with foodborne parasites have been obtained from different global regions, data from Colombia are lacking despite South America being a major producer of fresh produce, which...
Tue, 02/11/2020 - 03:25
Cyclosporiasis is caused by the coccidian parasite Cyclospora cayetanensis and is associated with large and complex food-borne outbreaks worldwide. Associated symptoms include severe watery diarrhea, particularly in infants, and immune dysfunction. With the globalization of human food supply, the occurrence of cyclosporiasis has been increasing in both food growing and importing countries. As...
Mon, 01/27/2020 - 03:34
Author(s): Tamirat Tefera Temesgen, Alessandra Barlaam, Kristoffer R. Tysnes, Lucy J. RobertsonAbstractThe potential public health impact of foodborne parasites transmitted via contaminated fresh produces indicates the necessity for robust and reliable laboratory methods for their detection and identification on this infection vehicle. Standardization of methods for detection of common FBP in...
Thu, 12/05/2019 - 04:08
Author(s): Angela Assurian, Helen Murphy, Laura Ewing, Hediye Nese Cinar, Alexandre da Silva, Sonia AlmeriaAbstractOutbreaks and sporadic cases of Cyclospora cayetanensis have been linked to consumption of berries. The efficacy of the U.S. Food and Drug Administration (FDA) method for detection of C. cayetanensis was evaluated in fresh berries (blackberries, strawberries, blueberries and mixed...
Wed, 11/20/2019 - 03:58
Author(s): Edoardo PozioAbstractFoodborne parasites, most of which are zoonotic, represent an important human health hazard. These pathogens which include both protozoa (e.g., Cryptosporidium spp., Cyclospora cayetanensis, Toxoplasma gondii) and helminths (e.g., liver and intestinal flukes, Fasciola spp., Paragonimus spp., Echinococcus spp., Taenia spp., Angiostrongylus spp., Anisakis spp.,...
Thu, 10/17/2019 - 03:32
This work assessed the risk of protozoa in 10 school restaurants in Armenia (Quindío, Colombia) by analyzing the presence of Cryptosporidium spp, Giardia duodenalis, Blastocystis, and Cyclospora cayetanensis DNA in the food, water, and living and inert surfaces of school restaurants and in stools of children who ate at these restaurants. Of the 213 food, water, and surface samples, 6.6% were...
Sun, 10/13/2019 - 03:37
Author(s): M.R. Gaafar, L.A. El-Zawawy, M.M. El-Temsahy, Th.I. Shalaby, A.Y. HassanAbstractCyclosporiasis is an emerging worldwide infection caused by an obligate intracellular protozoan parasite, Cyclospora cayetanensis. In immunocompetent patients, it is mainly manifested by self-limited diarrhea, which is persistent and may be fatal in immunocompromised patients. The standard treatment for...
Mon, 09/23/2019 - 03:30
Author(s): Tamirat Tefera Temesgen, Lucy Jane Robertson, Kristoffer Relling TysnesAbstractFoodborne parasites (FBP) are of major public health importance and warrant appropriate detection and control strategies. Most of the FBP considered for risk-ranking by a panel of experts are potentially transmitted via consumption of contaminated fresh produce, including berries. In this study we focused on...
Mon, 08/26/2019 - 20:00
Cyclospora cayetanensis is a coccidian parasite that has been associated with foodborne outbreaks of gastrointestinal illnesses. Raspberries have been implicated as vehicle of infection in some of these outbreaks. Most of the molecular techniques used for the detection of parasites commonly use the 18s rRNA as a target gene, which is highly conserved. The conserved nature of 18s rRNA gene among...
Fri, 08/23/2019 - 04:03
Author(s): Matthew M. Hitchcock, Catherine A. Hogan, Indre Budvytiene, Niaz BanaeiAbstractThough the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for...
Sun, 08/11/2019 - 01:04
Available online 9 August 2019 Author(s): Junqiang Li, Ke Shi, Fangfang Sun, Tingwen Li, Rongjun Wang, Sumei Zhang, Fuchun Jian, Changshen Ning, Longxian ZhangAbstractCryptosporidium spp., Giardia duodenalis, Cyclospora cayetanensis, and Enterocytozoon bieneusi are known etiological agents of self-limiting diarrhea, chronic disorders, and severe debilitating illnesses in humans, particularly...
Thu, 06/27/2019 - 01:14
Available online 24 June 2019 Author(s): Karen Shapiro, Minji Kim, Veronica B. Rajal, Michael J. Arrowood, Andrea Packham, Beatriz Aguilar, Stefan WuertzAbstractPathogen contamination of fresh produce presents a health risk for consumers; however, the produce industry still lacks adequate tools for simultaneous detection of protozoan parasites. Here, a simple multiplex PCR (mPCR) assay was...
Wed, 06/12/2019 - 00:00
F. S. Nascimento et al. Cyclosporiasis is an infection caused by Cyclospora cayetanensis, which is acquired by consumption of contaminated fresh food or water. In the United States, cases of cyclosporiasis are often associated with foodborne outbreaks linked to imported fresh produce or travel to disease-endemic countries. Epidemiologic investigation has been the primary method for linking...
Thu, 10/11/2018 - 20:00
Cyclospora cayetanensis is a food-borne intestinal human parasite that causes outbreaks of diarrhea. There is a need for efficient laboratory methods for strain-level characterization to assist in outbreak invest...
Sat, 04/14/2018 - 00:00
Publication date: 20 June 2018 , Volume 275 Author(s): Sophie Butot, Frédérique Cantergiani, Mireille Moser, Julie Jean, Anthony Lima, Lise Michot, Thierry Putallaz, Thomas Stroheker, Sophie Zuber Outbreaks of foodborne illness associated with berries often involve contamination with hepatitis A virus (HAV) and norovirus but also bacteria such as Escherichia coli O157:H7 and...
Thu, 08/24/2017 - 15:00
Publication date: February 2018 , Volume 69 Author(s): Helen R. Murphy, Hediye Nese Cinar, Gopal Gopinath, Kathy E. Noe, Lacresha D. Chatman, Nancy E. Miranda, June H. Wetherington, Jason Neal-McKinney, Gabrielle S. Pires, Elizabeth Sachs, Kristopher J. Stanya, Cynthia L. Johnson, Fernanda S. Nascimento, Monica Santin, Aleksey Molokin, Mansour Samadpour, Harish...
Tue, 06/13/2017 - 19:49
Publication date: October 2017 , Volume 67 Author(s): Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in ‘...
Wed, 06/07/2017 - 03:00
Journal of Food Protection, Volume 80, Issue 7, Page 1133-1144, July 2017. Cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with C. cayetanensis oocysts. In the United States, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro...
Sat, 10/15/2016 - 05:05
Publication date: November 2016 , Volume 130 Author(s): Fernanda S. Nascimento, Yuping Wei-Pridgeon, Michael J. Arrowood, Delynn Moss, Alexandre J. da Silva, Eldin Talundzic, Yvonne Qvarnstrom Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as...
Mon, 07/11/2016 - 00:00
Y. Guo et al. Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. This new tool could be useful for case linkage and infection/contamination source tracking.
Wed, 07/29/2015 - 01:24
Publication date: 15 October 2015 , Volume 211 Author(s): Annunziata Giangaspero, Marianna Marangi, Anson V. Koehler, R. Papini, G. Normanno, V. Lacasella, A. Lonigro, Robin B. Gasser To date, in Europe, there is scant information on the occurrence of Cyclospora in water from treatment plants and in humans, and no data are available on soil or fresh plant products. Here, we...
Sat, 05/09/2015 - 03:20
Publication date: Available online 9 May 2015 Author(s): Kjersti Selstad Utaaker , Qirong Huang , Lucy J. Robertson Fresh produce is a recognized vehicle for transmission of various protozoan parasites, including Toxoplasma gondii , Cyclospora caytenanensis , Giardia duodenalis , and Cryptosporidium spp. For Giardia and Cryptosporidium , a Standard ISO Method for analyzing fresh produce...