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Giardia lamblia

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Sun, 08/11/2019 - 01:04
Available online 9 August 2019 Author(s): Junqiang Li, Ke Shi, Fangfang Sun, Tingwen Li, Rongjun Wang, Sumei Zhang, Fuchun Jian, Changshen Ning, Longxian ZhangAbstractCryptosporidium spp., Giardia duodenalis, Cyclospora cayetanensis, and Enterocytozoon bieneusi are known etiological agents of self-limiting diarrhea, chronic disorders, and severe debilitating illnesses in humans, particularly...
Wed, 07/24/2019 - 01:07
Publication date: Available online 23 July 2019Source: Microbial Risk AnalysisAuthor(s): Ursula Gonzales-Barron, Anne Thébault, Pauline Kooh, Laurence Watier, Moez Sanaa, Vasco CadavezAbstractIn order to design effective public health strategies, and, in particular, effective food safety interventions to reduce the burden of foodborne disease, the most important sources of enteric illnesses...
Tue, 07/23/2019 - 20:00
Giardia lamblia, a single-celled eukaryote, colonizes and thrives in the small intestine of humans. Because of its compact and reduced genome, Giardia has adapted a “minimalistic” life style, as it becomes dependent on available resources of the small intestine. Because Giardia expresses fewer sphingolipid (SL) genes—and glycosphingolipids are critical for encystation—we investigated the SL...
Thu, 07/18/2019 - 13:19
Abstract Aims The objective of this study was to assess the probiotic potential of genuine strains of Bifidobacterium longum 51A and Weissella paramesenteroides WpK4, in experimental giardiasis. Methods and Results The bacteria were administered orally to gerbils (Meriones unguiculatus) 10 days before oral infection with trophozoites of Giardia lamblia. After 7 days of infection, the animals...
Thu, 06/27/2019 - 01:14
Available online 24 June 2019 Author(s): Karen Shapiro, Minji Kim, Veronica B. Rajal, Michael J. Arrowood, Andrea Packham, Beatriz Aguilar, Stefan WuertzAbstractPathogen contamination of fresh produce presents a health risk for consumers; however, the produce industry still lacks adequate tools for simultaneous detection of protozoan parasites. Here, a simple multiplex PCR (mPCR) assay was...
Tue, 05/21/2019 - 11:00
Giardia lamblia, one of the most common protozoal infections of the human intestine, is an important worldwide cause of diarrheal disease, malabsorption, malnutrition, delayed cognitive development in children, and protracted postinfectious syndromes. Despite its medical importance, no human vaccine is available against giardiasis. A crude veterinary vaccine has been developed, and experimental...
Sun, 03/17/2019 - 01:12
Available online 16 March 2019 Author(s): Panagiota Ligda, Edwin Claerebout, Lucy J. Robertson, Smaragda SotirakiAbstractMarine bivalve shellfish are of public health interest because they can accumulate pollutants in their tissues. As they are usually consumed raw or lightly cooked, they are considered to be a possible source of foodborne infections, including giardiosis and cryptosporidiosis....
Mon, 03/11/2019 - 17:00
Zi-Qi Lin, Soo-Wah Gan, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Chin-Hung Sun Giardia lamblia becomes dormant differentiation into a water-resistant cyst that can infect a new host. Synthesis of three cyst wall proteins (CWPs) is the fundamental feature of this differentiation. Myeloid leukemia factor (MLF) proteins are involved in cell differentiation, and tumorigenesis in mammals, but little is...
Fri, 07/06/2018 - 01:13
Publication date: November 2018 , Volume 93Author(s): Radka Dziedzinska, Monika Moravkova, Jakub Hrdy, Iva Slana, Hana Vlkova, Hana Kunstovna, Petra VasickovaAbstractFresh squeezed juices and smoothies currently enjoy great popularity among consumers. However, data regarding the microbiological safety of these products sold to the public in food outlets are either very limited or missing...
Thu, 05/03/2018 - 10:24
Publication date: Available online 2 May 2018 Author(s): Adriana Calderaro, Monica Martinelli, Mirko Buttrini, Sara Montecchini, Silvia Covan, Sabina Rossi, Francesca Ferraglia, Paolo Montagna, Federica Pinardi, Sandra Larini, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto This study represents a 2-year picture of the epidemiology...
Sat, 11/25/2017 - 06:22
Publication date: December 2017 , Volume 100, Issue 12 Author(s): Kathryn J. Boor, Martin Wiedmann, Sarah Murphy, Sam Alcaine Microbes that may be present in milk can include pathogens, spoilage organisms, organisms that may be conditionally beneficial (e.g., lactic acid bacteria), and those that have not been linked to either beneficial or detrimental effects on product quality or...
Fri, 10/06/2017 - 15:27
Publication date: 18 December 2017 , Volume 263 Author(s): Kjersti Selstad Utaaker, Anil Kumar, Himanshu Joshi, Suman Chaudhary, Lucy J. Robertson Fresh produce has been recognized as a vehicle of infection for protozoan parasites, particularly Cryptosporidium, and, to a lesser extent, Giardia. For both parasites, outbreaks associated with fresh produce have been documented....
Sun, 08/20/2017 - 07:30
Abstract AimsTo assess diarrheal risks from enteropathogenic E. coli, Giardia, and Cryptosporidium from consuming raw spinach, cabbage, carrots, and tomatoes in Kathmandu Valley, Nepal. Methods and ResultsThe annual infection risk was quantified using the probabilistic Quantitative Microbial Risk Assessment approach, which considered 12 vegetable washing combinations. A new model was used to...
Thu, 07/27/2017 - 17:00
Luther A. Bartelt, David T. Bolick, Jordi Mayneris-Perxachs, Glynis L. Kolling, Gregory L. Medlock, Edna I. Zaenker, Jeffery Donowitz, Rose Viguna Thomas-Beckett, Allison Rogala, Ian M. Carroll, Steven M. Singer, Jason Papin, Jonathan R. Swann, Richard L. Guerrant Diverse enteropathogen exposures associate with childhood malnutrition. To elucidate mechanistic pathways whereenteric microbes...
Tue, 06/13/2017 - 19:49
Publication date: October 2017 , Volume 67 Author(s): Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in ‘...
Sat, 06/03/2017 - 09:24
Publication date: 16 August 2017 , Volume 255 Author(s): Kjersti Selstad Utaaker, Eystein Skjerve, Lucy J. Robertson Fresh produce has been recognized as a vehicle for transmission of protozoan parasites for many years, and there are numerous publications regarding their occurrence on such foodstuffs, indicating their potential importance as foodborne parasites. Nevertheless, few...
Wed, 04/12/2017 - 03:00
Journal of Food Protection, Volume 80, Issue 5, Page 837-841, May 2017.
Tue, 03/07/2017 - 17:00
Mario Santoro, Nicola D’Alessio, Anna Cerrone, Maria Gabriella Lucibelli, Giorgia Borriello, Gaetano Aloise, Clementina Auriemma, Nunzia Riccone, Giorgio Galiero Canine monocytic ehrlichiosis and rickettsiosis are zoonotic tick-borne diseases of canids caused the intracellular obligate bacteria Ehrlichia canis and Rickettsia species respectively. In this study, we investigated using standard and...
Thu, 03/02/2017 - 21:53
Publication date: Available online 2 March 2017 Source:Microbial Risk Analysis Author(s): Mary E. Schoen, Nicholas J. Ashbolt, Michael A. Jahne, Jay Garland This paper presents risk-based enteric pathogen log reduction targets for non-potable and potable uses of a variety of alternative source waters (i.e., locally-collected greywater, roof runoff, and stormwater). A probabilistic...
Mon, 01/16/2017 - 17:19
Publication date: Available online 16 January 2017 Author(s): Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones In this study, the use of skimmed milk flocculation (SMF) to...
Fri, 01/06/2017 - 13:32
Publication date: Available online 9 November 2016 Source:Microbial Risk Analysis Author(s): Michael A. Jahne, Mary E. Schoen, Jay L. Garland, Nicholas J. Ashbolt As decentralized water reuse continues to gain popularity, risk-based treatment guidance is increasingly sought for the protection of public health. However, efforts to evaluate pathogen risks and log-reduction requirements...
Fri, 11/18/2016 - 17:00
Eun Jeong Won, Soo Hyun Kim, Seung Jung Kee, Jong Hee Shin, Soon Pal Suh, Jong Yil Chai, Dong Wook Ryang, Myung Geun Shin Intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. We developed a multiplex real-time PCR assay for detection of eight intestinal parasites and...
Sat, 10/15/2016 - 14:10
Publication date: 15 November 2016 , Volume 195 Author(s): Mirjam Duijvestijn, Lapo Mughini-Gras, Nancy Schuurman, Wim Schijf, Jaap A. Wagenaar, Herman Egberink Laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. The presence of potential pathogens however, does not prove a causal relationship with diarrhoea. The aim of...
Fri, 09/23/2016 - 12:01
Giardia lamblia is a pathogen transmitted by water and food that causes infection worldwide. Giardia genotypes are classified into 8 assemblages (A–H). Assemblages A and B are detected in humans, but they are potentially zoonotic because they infect other mammalian hosts. Giardia in samples from 44 children was genotyped. Conserved fragments of the genes encoding β-giardin and...
Wed, 07/20/2016 - 17:00
Jon Paulin Zumthor, Lenka Cernikova, Samuel Rout, Andres Kaech, Carmen Faso, Adrian B. Hehl Giardia lamblia is a parasitic protozoan that infects a wide range of vertebrate hosts including humans. Trophozoites are non-invasive but associate tightly with the enterocyte surface of the small intestine. This narrow ecological specialization entailed extensive morphological and functional adaptations...
Thu, 06/30/2016 - 11:01
Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in...
Wed, 06/22/2016 - 17:00
Xian-Min Feng, Wen-Yu Zheng, Hong-Mei Zhang, Wen-Yan Shi, Yao Li, Bai-Ji Cui, Hui-Yan Wang Background Giardia lamblia is one of the most common infectious protozoans in human that may cause diarrhea in travelers. Searching for antigens that induced effectively protective immunity has become a key point in the development of vaccine against giardiasis. Methodology/Principal Findings Mice...
Thu, 06/16/2016 - 14:01
Publication date: July 2016 , Volume 126 Author(s): M. Jimenez, H. Bridle We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also...
Fri, 04/08/2016 - 20:48
Publication date: August 2016 , Volume 57 Author(s): Jeanne Hohweyer, Catherine Cazeaux, Emmanuelle Travaillé, Emilie Languet, Aurélien Dumètre, Dominique Aubert, Christine Terryn, Jitender P. Dubey, Nadine Azas, Maryline Houssin, Favennec Loïc, Isabelle Villena, Stéphanie La Carbona Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging...
Fri, 12/18/2015 - 08:28
Summary Although pets provide physiological and psychological benefits to their owners, they are a potential source of zoonotic infections, especially for vulnerable individuals such as immunocompromised patients. During 1 year, we therefore performed a pilot project, which included 32 immunocompromised Chilean children and their family pets (35 dogs and 9 cats) with the aim of detecting,...
Thu, 12/03/2015 - 17:00
Sara M. Pires, Christa L. Fischer-Walker, Claudio F. Lanata, Brecht Devleesschauwer, Aron J. Hall, Martyn D. Kirk, Ana S. R. Duarte, Robert E. Black, Frederick J. Angulo Background Diarrhoeal diseases are major contributors to the global burden of disease, particularly in children. However, comprehensive estimates of the incidence and mortality due to specific aetiologies of diarrhoeal diseases...
Mon, 11/23/2015 - 18:57
Publication date: Available online 10 November 2015 Author(s): Gregoire Seyrig, Robert D. Stedtfeld, Dieter M. Tourlousse, Farhan Ahmad, Keara Towery, Alison M. Cupples, James M. Tiedje, Syed A. Hashsham Loop-mediated isothermal amplification (LAMP) is increasingly used for point-of-care nucleic acid based diagnostics. LAMP can be monitored in real-time by measuring the...
Wed, 11/04/2015 - 09:16
Abstract AimsThis study investigated waterborne opportunistic pathogens (OPs) including potential hosts, and evaluated the use of Legionella spp. for indicating microbial water quality for OPs within a full-scale operating drinking water distribution system (DWDS). Methods and ResultsTo investigate the occurrence of specific microbial pathogens within a major city DWDS we examined large volume (...
Mon, 09/28/2015 - 03:29
Canada geese (Branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. Further, increases in both the Canada geese populations and their ideal habitat requirements in the United States (US) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. Combining these...
Sat, 08/08/2015 - 01:19
Diarrhea causes significant morbidity and mortality in Indian children under 5 years of age. Flies carry enteric pathogens and may mediate foodborne infections. In this study, we characterized fly densities as a determinant of infectious diarrhea in a longitudinal cohort of 160 urban and 80 rural households with 1,274 individuals (27% under 5 years of age) in Vellore, India. Household...
Fri, 07/17/2015 - 01:19
Publication date: January 2016 , Volume 59 Author(s): Emmanuelle Travaillé , Stéphanie La Carbona , Gilles Gargala , Dominique Aubert , Karine Guyot , Aurélien Dumètre , Isabelle Villena , Maryline Houssin This study evaluates the viability of Giardia intestinalis cysts , Cryptosporidium spp. and Toxoplasma gondii oocysts, which may be found in water and food matrices subjected to...
Thu, 05/14/2015 - 03:20
e-First Articles. In this study, we determined the repair capabilities of Giardia lamblia cysts when they were exposed to low-pressure (LP) UV and then 4 different repair conditions. A UV collimated beam apparatus was used to expose shallow suspensions of G. lamblia cysts in buffered reagent water (PBS, pH 7.2) to various doses of LP UV irradiation. After UV irradiation, samples were exposed to...
Sun, 05/10/2015 - 01:14
Catrin E. Moore, Phot Nget, Mao Saroeun, Suy Kuong, Seng Chanthou, Varun Kumar, Rachel Bousfield, Johanna Nader, J. Wendi Bailey, Nicholas J. Beeching, Nicholas P. Day, Christopher M. Parry Background Infections with helminths and other intestinal parasites are an important but neglected problem in children in developing countries. Accurate surveys of intestinal parasites in children inform...
Sat, 05/09/2015 - 03:20
Publication date: Available online 9 May 2015 Author(s): Kjersti Selstad Utaaker , Qirong Huang , Lucy J. Robertson Fresh produce is a recognized vehicle for transmission of various protozoan parasites, including Toxoplasma gondii , Cyclospora caytenanensis , Giardia duodenalis , and Cryptosporidium spp. For Giardia and Cryptosporidium , a Standard ISO Method for analyzing fresh produce...
Sat, 04/18/2015 - 01:21
Aim To examine the occurrence and quantity of potential pathogens and an indicator of microbial contamination in the sediments of municipal drinking water storage tanks (MDWSTs), given the absence of such data across the United States. Methods and Results Sediment samples (87 MDWST) from eighteen locations across ten states of the U.S. were collected and assayed by qPCR for a range of potential...
Fri, 01/30/2015 - 00:08
Publication date: March 2015 , Volume 110 Author(s): Dorien Van den Bossche , Lieselotte Cnops , Jacob Verschueren , Marjan Van Esbroeck Purpose Microscopy is the diagnostic reference standard for the detection of parasites, but it is labor-intensive and requires experience. Rapid diagnostic tests (RDTs) can provide an alternative to microscopy. Methods RDTs from four different...
Tue, 01/20/2015 - 14:53
Paediatric acute gastroenteritis is a global public health problem.Comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections.Our study aimed to investigate the role of various pathogens in infantile diarrhoea in Bulgaria...