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PLoS One

Mon, 08/13/2018 - 17:00
Fernanda Almeida, Amanda Aparecida Seribelli, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Alessandro de MelloVarani, Yan Luo, Marc W. Allard, Juliana Pfrimer Falcão Whole genome sequencing (WGS) has been used as a powerful technology for molecular epidemiology, surveillance, identification of species and serotype, identification of the sources of outbreaks, among other purposes...
Fri, 08/10/2018 - 17:00
Ross C. Beier, Roger B. Harvey, Charles A. Hernandez, Michael E. Hume, Kathleen Andrews, Robert E. Droleskey, Maureen K. Davidson, Sonia Bodeis-Jones, Shenia Young, Sara E. Duke, Robin C. Anderson, Tawni L. Crippen, Toni L. Poole, David J. Nisbet Campylobacter coli is a bacterial species that is a major cause of diarrheal disease worldwide, and Campylobacter spp. are among the top 5 foodborne...
Wed, 08/08/2018 - 17:00
Carolina M. Bedoya-Serna, Euder C. Michelin, Marina M. Massocco, Lucas C. S. Carrion, Silvia H. S. Godoy, Cesar G. Lima, Paulo S. Ceccarelli, George S. Yasui, George E. Rottinghaus, Ricardo L. M. Sousa, Andrezza M. Fernandes Aflatoxins (AF) can be cumulative in fish tissues and can influence weight, length, feed intake and survival depending on the species. The aim of this work is to measure...
Fri, 08/03/2018 - 17:00
Mark A. Schmidt, Holly C. Groom, Allison L. Naleway, Christianne Biggs, S. Bianca Salas, Kayoko Shioda, Zachary Marsh, Judy L. Donald, Aron J. Hall Background This study presents a novel methodology for estimating all-age, population-based incidence rates of norovirus and other pathogens that contribute to acute gastroenteritis in the United States using an integrated healthcare delivery system...
Thu, 07/26/2018 - 17:00
Geethika Reddi, Kali Pruss, Kathryn L. Cottingham, Ronald K. Taylor, Salvador Almagro-Moreno Vibrio cholerae O1, the etiological agent of cholera, is a natural inhabitant of aquatic ecosystems. Motility is a critical element for the colonization of both the human host and its environmental reservoirs. In this study, we investigated the molecular mechanisms underlying the chemotactic response of...
Wed, 07/25/2018 - 17:00
Tonya M. Santaus, Shan Li, Paula Ladd, Amanda Harvey, Shannon Cole, O. Colin Stine, Chris D. Geddes Sample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It® for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio cholerae. The concept of Lyse-It® employs a...
Mon, 07/23/2018 - 17:00
Soleil E. Worthy, Lillian Haynes, Melissa Chambers, Danika Bethune, Emily Kan, Kevin Chung, Ryan Ota, Charles J. Taylor, Elizabeth E. Glater Food choice is critical for survival because organisms must choose food that is edible and nutritious and avoid pathogenic food. Many organisms, including the nematode C. elegans, use olfaction to detect and distinguish among food sources. C. elegans...
Thu, 07/19/2018 - 17:00
Helen Kathleen Crabb, Joanne Lee Allen, Joanne Maree Devlin, Simon Matthew Firestone, Colin Reginald Wilks, James Rudkin Gilkerson The transmission of Salmonella enterica within a vertically integrated poultry operation was investigated longitudinally over an 18-month period (2013–2014). Thirty six percent of all samples collected (1503 of 4219) were positive for salmonellae with seven...
Wed, 07/18/2018 - 17:00
Mia Madel Alfajaro, Eun-Hyo Cho, Jun-Gyu Park, Ji-Yun Kim, Mahmoud Soliman, Yeong-Bin Baek, Mun-Il Kang, Sang-Ik Park, Kyoung-Oh Cho Cyclooxygenases (COXs)/prostaglandin E2 (PGE2) signaling pathways are known to modulate a variety of homeostatic processes and are involved in various pathophysiological conditions. COXs/PGE2 signaling pathways have also been demonstrated to have proviral or...
Wed, 07/18/2018 - 17:00
Can Chen, Jian-Bo Yan, Hong-Ling Wang, Peng Li, Ke-Feng Li, Bing Wu, Hui Zhang A total of 1 590 fecal swabs and stool samples from sporadic acute gastroenteritis patients of all ages were collected from January 2013 to March 2018 in the Zhoushan Islands, China, with 99 (6.23%) samples subsequently identified as human norovirus (HuNoV) positive. Phylogenetic analysis of partial RdRp and VP1 gene...
Thu, 07/12/2018 - 17:00
Anna Irene Vedel Sørensen, Thomas Rosendal, Stefan Widgren, Tariq Halasa Knowledge on successful interventions against livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) within pig herds is sparse. In situations like this, a mechanistic simulation model can be a valuable tool for assessing the effect of potential intervention strategies, and prioritising which should be...
Thu, 07/05/2018 - 17:00
Ekaterina Pokrant, Francisca Medina, Aldo Maddaleno, Betty San Martín, Javiera Cornejo Several antimicrobials are routinely used the poultry farming industry on their daily operations, however, researchers have found for some antimicrobials that their residues persist for longer periods in feathers than they do in edible tissues, and at higher concentrations, as well. But this information is not...
Tue, 07/03/2018 - 17:00
Uyen T. Nguyen, Sara A. Lincoln, Ana Gabriela Valladares Juárez, Martina Schedler, Jennifer L. Macalady, Rudolf Müller, Katherine H. Freeman A significant portion of oil released during the Deepwater Horizon disaster reached the Gulf of Mexico (GOM) seafloor. Predicting the long-term fate of this oil is hindered a lack of data about the combined influences of pressure, temperature, and sediment...
Tue, 07/03/2018 - 17:00
Maximilian B. Maier, Tobias Schweiger, Christian A. Lenz, Rudi F. Vogel The effect of high pressure thermal (HPT) treatments on the inactivation of spores of non-proteolytic type E Clostridium botulinum TMW 2.990 was investigated at high pressures (300 to 600 MPa) and elevated temperatures (80 to 100 °C) in four low-acid foods (steamed sole, green peas with ham, vegetable soup, braised veal) and...
Thu, 06/28/2018 - 17:00
Kelly E. Davidson, Barbara A. Byrne, Alda F. A. Pires, K. Gary Magdesian, Richard V. Pereira Nontyphoidal Salmonella infections contribute to approximately 1.2 million annual illnesses in the United States. Historical and recent outbreaks have been associated with dairy products, ground beef, and direct contact with cattle. Salmonella antimicrobial resistance (AMR) is a serious concern that can...
Thu, 06/14/2018 - 17:00
Ameenat Lola Solebo, Anne-Marie Austin, Maria Theodorou, Chris Timms, Joanne Hancox, Gillian G. W. Adams Background Botulinum toxin injection chemodenervation is a well-established intervention for adult strabismus, and has also been recognised as an effective alternative to routine incisional surgery for paediatric disease. We aimed to investigate the temporal patterns of practice, indications...
Mon, 06/11/2018 - 17:00
Anouk M. Oordt-Speets, Renee Bolijn, Rosa C. van Hoorn, Amit Bhavsar, Moe H. Kyaw Bacterial meningitis is a global public health concern, with several responsible etiologic agents that vary age group and geographical area. The aim of this systematic review and meta-analysis was to assess the etiology of bacterial meningitis in different age groups across global regions. PubMed and EMBASE were...
Fri, 06/08/2018 - 17:00
Sara Lomonaco, Matthew A. Crawford, Christine Lascols, Ruth E. Timme, Kevin Anderson, David R. Hodge, Debra J. Fisher, Segaran P. Pillai, Stephen A. Morse, Erum Khan, Molly A. Hughes, Marc W. Allard, Shashi K. Sharma The emergence and dissemination of carbapenemases, bacterial enzymes able to inactivate most β-lactam antibiotics, in Enterobacteriaceae is of increasing concern. The concurrent...
Tue, 06/05/2018 - 17:00
Michela Sali, Flavio De Maio, Michela Tarantino, Giuliano Garofolo, Manuela Tittarelli, Lorena Sacchini, Katiuscia Zilli, Paolo Pasquali, Paola Petrucci, Cinzia Marianelli, Massimiliano Francia, Maurizio Sanguinetti, Rosanna Adone Brucellosis is essentially a disease of domesticated livestock; however, humans can also be infected via the consumption of contaminated meat or dairy products,...
Tue, 06/05/2018 - 17:00
Tryntsje Cuperus, Marina D. Kraaij, Aldert L. Zomer, Albert van Dijk, Henk P. Haagsman Host Defense Peptides (HDPs) such as cathelicidins are multifunctional effectors of the innate immune system with both antimicrobial and pleiotropic immunomodulatory functions. Chicken cathelicidin-2 (CATH-2) has multiple immunomodulatory effects in vitro and the D-amino acid analog of this peptide has been...
Fri, 06/01/2018 - 17:00
Kristina J. Betz, Elizabeth A. Maier, Surya Amarachintha, David Wu, Erik P. Karmele, Jeremy M. Kinder, Kris A. Steinbrecher, Monica M. McNeal, Deborah H. Luzader, Simon P. Hogan, Sean R. Moore Background Polymeric immunoglobulin receptor (pIgR) transport of secretory immunoglobulin A (SIgA) to mucosal surfaces is thought to promote gut integrity and immunity to Salmonella enterica serovar...
Fri, 06/01/2018 - 17:00
Emily Sellens, Jacqueline M. Norris, Navneet K. Dhand, Jane Heller, Lynne Hayes, Heather F. Gidding, Harold Willaby, Nicholas Wood, Katrina L. Bosward Q fever vaccine uptake among veterinary nurses in Australia is low, suggesting veterinarians are not recommending the vaccination to veterinary personnel. This study aimed to determine the willingness of veterinarians to recommend Q fever...
Thu, 05/31/2018 - 17:00
Marisa Hohnadel, Myriam Maumy, Renaud Chollet For nearly a century, conventional microbiological methods have been standard practice for detecting and identifying pathogens in food. Nevertheless, the microbiological safety of food has improved and various rapid methods have been developed to overcome the limitations of conventional methods. Alternative methods are expected to detect low cell...
Fri, 05/25/2018 - 17:00
Marion Girard, Sophie Thanner, Nicolas Pradervand, Dou Hu, Catherine Ollagnier, Giuseppe Bee An experimental model for postweaning diarrhea with enterotoxigenic Escherichia coli F4 (ETEC F4) was set up in piglets, and the efficacy of 1% chestnut-tannin extract in preventing diarrhea was subsequently assessed. In a first trial (infection model), 32 Swiss Large White piglets (age: 24 days; average...
Fri, 05/25/2018 - 17:00
Solveig Sølverød Mo, Anne Margrete Urdahl, Knut Madslien, Marianne Sunde, Live L. Nesse, Jannice Schau Slettemeås, Madelaine Norström The objective of this study was to estimate and compare the occurrence of AMR in wild red foxes in relation to human population densities. Samples from wild red foxes (n = 528) included in the Norwegian monitoring programme on antimicrobial resistance in bacteria...
Wed, 05/23/2018 - 17:00
Dustin C. Ernst, Andrew J. Borchert, Diana M. Downs Microorganisms respond to a variety of metabolic perturbations repurposing or recruiting pathways to reroute metabolic flux and overcome the perturbation. Elimination of the 2-dehydropantoate 2-reductase, PanE, both reduces total coenzyme A (CoA) levels and causes a conditional HMP-P auxotrophy in Salmonella enterica. CoA or acetyl-CoA has no...
Tue, 05/22/2018 - 17:00
Marek Łukasz Roszko, Marta Kamińska, Krystyna Szymczyk, Katarzyna Piasecka-Jóźwiak, Beata Chabłowska The aim of this work was to evaluate the activity of xenobiotic mixtures containing persistent organic pollutants isolated from commercial fish oil samples against sex hormone receptors, including estrogen and androgen. The applied bioassay was based on transgenic yeast strains. The mixtures were...
Mon, 05/21/2018 - 17:00
Khondoker Abdul Mottaleb, Pawan Kumar Singh, Kai Sonder, Gideon Kruseman, Thakur Prasad Tiwari, Naresh C. D. Barma, Paritosh Kumar Malaker, Hans-Joachim Braun, Olaf Erenstein New biotic stresses have emerged around the globe over the last decades threatening food safety and security. In 2016, scientists confirmed the presence of the devastating wheat-blast disease in Bangladesh, South Asia–its...
Mon, 05/21/2018 - 17:00
Victor Neira, Matt Allerson, Cesar Corzo, Marie Culhane, Aaron Rendahl, Montserrat Torremorell The 2009 influenza pandemic, the variant H3N2v viruses in agricultural fairs and the zoonotic poultry H5N9 infections in China have highlighted the constant threat that influenza A viruses (IAV) present to people and animals. In this study we evaluated the effect of IAV vaccination on aerosol shedding...
Wed, 05/16/2018 - 17:00
Tilen Koklic, Štefan Pintarič, Irena Zdovc, Majda Golob, Polona Umek, Alma Mehle, Martin Dobeic, Janez Štrancar High economic burden is associated with foodborne illnesses. Different disinfection methods are therefore employed in food processing industry; such as use of ultraviolet light or usage of surfaces with copper-containing alloys. However, all the disinfection methods currently in use...
Wed, 05/16/2018 - 17:00
Isabel Krug, Matthias Behrens, Melanie Esselen, Hans-Ulrich Humpf Enniatins are common contaminants of food and feed and belong to the group of the “emerging” mycotoxins, which are produced various Fusarium species. Although a wide range of toxic effects, like antibacterial, antifungal, insecticidal and cytotoxic properties, have been described in vitro, so far, no cases of mycotoxicosis...
Mon, 05/14/2018 - 17:00
Jagamya Vijayaraghavan, Vijay Kumar, Nikhil P. Krishnan, Ross T. Kaufhold, Ximin Zeng, Jun Lin, Focco van den Akker The bacterial soluble lytic transglycosylase (LT) breaks down the peptidoglycan (PG) layer during processes such as cell division. We present here crystal structures of the soluble LT Cj0843 from Campylobacter jejuni with and without bulgecin A inhibitor in the active site. Cj0843...
Mon, 05/14/2018 - 17:00
Kara B. Marley, Larry A. Kuehn, John W. Keele, Benjamin W. Wileman, Michael G. Gonda Individuals often respond differently to the same vaccine; some of this variation may be caused genetic differences among animals. Our objective was to estimate heritability and identify genomic regions associated with humoral response to an Escherichia coli O157:H7 vaccine in beef cattle. Crossbred beef cattle...
Wed, 05/09/2018 - 17:00
Zhila Esna Ashari, Nairanjana Dasgupta, Kelly A. Brayton, Shira L. Broschat Type IV secretion systems (T4SS) are multi-protein complexes in a number of bacterial pathogens that can translocate proteins and DNA to the host. Most T4SSs function in conjugation and translocate DNA; however, approximately 13% function to secrete proteins, delivering effector proteins into the cytosol of eukaryotic...
Wed, 05/09/2018 - 17:00
Seon Woo Kim, Julie Haendiges, Eric N. Keller, Robert Myers, Alexander Kim, Jason E. Lombard, Jeffrey S. Karns, Jo Ann S. Van Kessel, Bradd J. Haley Unpasteurized dairy products are known to occasionally harbor Listeria monocytogenes and have been implicated in recent listeriosis outbreaks and numerous sporadic cases of listeriosis. However, the diversity and virulence profiles of L....
Wed, 05/09/2018 - 17:00
Friederike Raczkowski, Anne Rissiek, Isabell Ricklefs, Kirsten Heiss, Valéa Schumacher, Kira Wundenberg, Friedrich Haag, Friedrich Koch-Nolte, Eva Tolosa, Hans-Willi Mittrücker The ectoenzymes CD39 and CD73 degrade extracellular ATP to adenosine. ATP is released stressed or damaged cells and provides pro-inflammatory signals to immune cells through P2 receptors. Adenosine, on the other hand,...
Wed, 05/09/2018 - 17:00
Haiwei Cheng, Yi Yang, Yifei Chen, Xueqiu Chen, Zizheng Cai, Aifang Du Citrinin (CIT) is a hepato-nephrotoxic fungal metabolite produced the genera Penicillium, Aspergillus and Monascu. There is an increasing demand for rapid and economical methods for detection CIT residues in fruit. In this study, we developed an immunochromatographic strip (ICS) for detection of citrinin (CIT) residues in...
Tue, 05/08/2018 - 17:00
Sohyun Cho, Lari M. Hiott, John B. Barrett, Elizabeth A. McMillan, Sandra L. House, Shaheen B. Humayoun, Eric S. Adams, Charlene R. Jackson, Jonathan G. Frye Surface waters are important sources of water for drinking, industrial, agricultural, and recreational uses; hence, contamination of water fecal, pathogenic, or antimicrobial resistant (AR) bacteria is a major environmental and public...
Thu, 05/03/2018 - 17:00
Metadel Adane, Brhanu Teka, Yirga Gismu, Goitom Halefom, Muluneh Ademe Introduction In sub-Saharan Africa foodborne disease and foodborne disease outbreaks are frequently ascribed to poor personal hygiene of street food vendors and food handlers in food establishments. Evidence on the level of food hygiene practices and food safety measures among food handlers is required for proper planning and...
Mon, 04/30/2018 - 17:00
Pragathi B. Shridhar, Isha R. Patel, Jayanthi Gangiredla, Lance W. Noll, Xiaorong Shi, Jianfa Bai, Christopher A. Elkins, Nancy Strockbine, T. G. Nagaraja Escherichia coli O104:H4, a hybrid pathotype reported in a large 2011 foodborne outbreak in Germany, has not been detected in cattle feces. However, cattle harbor and shed in the feces other O104 serotypes, particularly O104:H7, which has been...
Wed, 04/25/2018 - 17:00
Tatiane Dobrzanski, Fernanda Gravina, Bruna Steckling, Luiz R. Olchanheski, Ricardo F. Sprenger, Bruno C. Espírito Santo, Carolina W. Galvão, Péricles M. Reche, Rosilene A. Prestes, Sônia A. V. Pileggi, Francinete R. Campos, Ricardo A. Azevedo, Michael J. Sadowsky, Flávio L. Beltrame, Marcos Pileggi The intense use of herbicides for weed control in agriculture causes selection pressure on soil...
Tue, 04/24/2018 - 17:00
Fuxun Ai, Nico Eisenhauer, Yuwei Xie, Jianguo Zhu, Alexandre Jousset, Wenchao Du, Ying Yin, Xiaowei Zhang, Rong Ji, Hongyan Guo The concentration of atmospheric carbon dioxide (CO2) and polycyclic aromatic hydrocarbons (PAHs) contents in the environment have been rising due to human activities. Elevated CO2 (eCO2) levels have been shown to affect plant physiology and soil microbes, which may...
Thu, 04/19/2018 - 17:00
Rafael Campos, Fabíola Z. Mónica, Alberto Fernando Oliveira Justo, José Carlos Cogo, Edvana de Toledo Oliveira, Ronilson Agnaldo Moreno, Edson Antunes, Gilberto De Nucci A tetrodotoxin (TTX)-resistant mechanism is responsible for the electrical field stimulation (EFS)-induced contractions and relaxations of Crotalus durissus terrificus corpora cavernosa. Here it was investigated whether this...
Wed, 04/18/2018 - 17:00
Samar S. Boswihi, Edet E. Udo, Stefan Monecke, Bindu Mathew, BobNoronha, Tina Verghese, Sajida B. Tappa Background Frequent changes in the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) occurring worldwide demand regular surveillance to study their composition and distribution in healthcare facilities. We investigated the genotypic characteristics of MRSA obtained in Kuwait...
Wed, 04/18/2018 - 17:00
Muhammet Karakavuk, Duygu Aldemir, Aurélien Mercier, Esra Atalay Şahar, Hüseyin Can, Jean-Benjamin Murat, Ömer Döndüren, Şengül Can, Hüseyin Gökhan Özdemir, Aysu Değirmenci Döşkaya, Bayram Pektaş, Marie-Laure Dardé, Adnan Yüksel Gürüz, Mert Döşkaya Toxoplasma gondii is a protozoon parasite that causes congenital toxoplasmosis, as well as other serious clinical presentations, in immune...
Mon, 04/16/2018 - 17:00
Nadezhda A. Wall, C. Coral Dominguez-Medina, Sian E. Faustini, Charlotte N. Cook, Andrew McClean, Mark D. Jesky, Marisol Perez-Toledo, Matthew D. Morgan, Alexandra G. Richter, Charles J. Ferro, Paul Cockwell, Paul A. Moss, Ian R. Henderson, Lorraine Harper, Adam F. Cunningham Patients with chronic kidney disease (CKD) have an increased risk of infection and poorer responses to vaccination. This...
Thu, 04/12/2018 - 17:00
Sarah-Jo Paquette, Kim Stanford, James Thomas, Tim Reuter Often Escherichia coli are harmless and/or beneficial bacteria inhabiting the gastrointestinal tract of livestock and humans. However, Shiga toxin-producing E. coli (STEC) have been linked to human disease. Cattle are the primary reservoir for STEC and STEC “super-shedders” are considered to be a major contributor in animal to animal...
Fri, 04/06/2018 - 17:00
Hyesun Jang, Mohamed Elaish, Mahesh KC, Michael C. Abundo, Amir Ghorbani, John M. Ngunjiri, Chang-Won Lee Outbreaks of novel highly pathogenic avian influenza viruses have been reported in poultry species in the United States since 2014. These outbreaks have proven the limitations of biosecurity control programs, and new tools are needed to reinforce the current avian influenza control arsenal....
Thu, 04/05/2018 - 17:00
Laura Adamovicz, Matthew C. Allender, Grace Archer, Marta Rzadkowska, Kayla Boers, Chris Phillips, Elizabeth Driskell, Michael J. Kinsel, Caroline Chu Wildlife mortality investigations are important for conservation, food safety, and public health; but they are infrequently reported for cryptic chelonian species. Eastern box turtles (Terrapene carolina carolina) are declining due to...
Wed, 04/04/2018 - 17:00
Joachim Spergser, Igor Loncaric, Alexander Tichy, Johannes Fritz, Alexandra Scope The critically endangered Northern bald ibis (Geronticus eremita) is a migratory bird that became extinct in Europe centuries ago. Since 2014, the Northern bald ibis is subject to an intensive rehabilitation and conservation regime aiming to reintroduce the bird in its original distribution range in Central Europe...

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