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Norovirus

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Thu, 07/25/2019 - 20:00
With the help of an existing citywide comprehensive surveillance on gastroenteritis outpatients, although norovirus genogroup II (NoV GII) was tested routinely, its genotypes were never investigated systematic...
Wed, 07/24/2019 - 01:07
Publication date: Available online 23 July 2019Source: Microbial Risk AnalysisAuthor(s): Ursula Gonzales-Barron, Anne Thébault, Pauline Kooh, Laurence Watier, Moez Sanaa, Vasco CadavezAbstractIn order to design effective public health strategies, and, in particular, effective food safety interventions to reduce the burden of foodborne disease, the most important sources of enteric illnesses...
Wed, 07/24/2019 - 00:00
Abstract Detection of noroviruses in bivalve shellfish is difficult because of the low concentration of norovirus and the presence of reverse transcription (RT)-PCR inhibitors. This study aimed to assess the presence of noroviruses in oysters extracted using a proteinase K extraction (ISO 15216 method) and an adsorption–elution method. Seventy oyster samples were extracted using...
Mon, 07/22/2019 - 17:00
Bridget A. Robinson, Jacob A. Van Winkle, Broc T. McCune, A. Mack Peters, Timothy J. Nice Human norovirus (HNoV) is the leading cause of acute gastroenteritis and is spread fecal shedding that can often persist for weeks to months after the resolution of symptoms. Elimination of persistent viral reservoirs has the potential to prevent outbreaks. Similar to HNoV, murine norovirus (MNV) is spread...
Wed, 07/03/2019 - 01:06
Available online 1 July 2019 Author(s): Danielle Leblanc, Marie-Josée Gagné, Élyse Poitras, Julie BrassardAbstractThe viability of murine norovirus (MNV-1), bovine rotavirus (boRV), and hepatitis A virus (HAV) was evaluated at 21 °C, 4 °C, and −20 °C on stainless steel surfaces, in bottled water, and on blueberries for up to 21 days. After 14 days of incubation at 21 °C on stainless steel, a...
Sun, 06/30/2019 - 01:12
Available online 28 June 2019 Author(s): Menka Suresh, Jennifer Harlow, Neda NasheriAbstractLeafy vegetables and fresh herbs are important parts of a healthy diet, however, they can be common vehicles of norovirus (NoV) infection and lead to serious health and economic concerns globally. NoV is highly infectious and persistent in the food and the environment, while being resistant to conventional...
Tue, 06/25/2019 - 13:52
Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-Å) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and...
Thu, 06/20/2019 - 14:39
Abstract Although norovirus (NoV) is the major cause of gastroenteritis, with the largest number of NoV food poisoning cases in Japan, limited information is available regarding NoV detection in food. This study aimed to detect NoV in food samples during the 2015–2016 suspected foodborne outbreaks in Tokyo; 352 food samples from 64 NoV food poisoning outbreaks were collected. Bacterial culturing...
Sat, 06/15/2019 - 01:07
Available online 13 June 2019 Author(s): Yong Ma, Sujuan Ding, Yanquan Fei, Gang Liu, Hongmei Jang, Jun FangAbstractThe main foodborne pathogens are Norovirus, Enteric parasites, bacteria and fungi. Foodborne diseases can lead to high morbidity and mortality and result in huge economic losses. Some foodborne diseases are associated with antibiotic resistance, which is a significant problem...
Thu, 06/06/2019 - 01:11
Available online 4 June 2019 Author(s): Andreia I. Pimenta, Fernanda M.A. Margaça, Sandra Cabo VerdeAbstractThe environmental stability of enteric viruses and resistance to conventional treatments and common disinfectants, leads to their persistence in waters and food, causing serious implications on public health. Among non-thermal treatment methods, ionizing radiation is recognized as a useful...
Wed, 06/05/2019 - 00:00
Abstract Human noroviruses are the leading cause of viral gastroenteritis. In the absence of a practical culture technique for routine analysis of infectious noroviruses, several methods have been developed to discriminate between infectious and non-infectious viruses by removing non-viable viruses prior to analysis by RT-qPCR. In this study, two such methods (RNase and porcine...
Thu, 05/23/2019 - 20:00
Human norovirus (HuNoV) is the major cause of viral acute gastroenteritis for all age groups in various countries. HuNoV GII in particular accounted for the majority of norovirus outbreaks, among which GII.4 c...
Tue, 05/21/2019 - 00:00
Abstract Contamination of bivalve shellfish, particularly oysters, with norovirus is recognised as a significant food safety risk. Methods for quantification of norovirus in oysters using the quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) are well established, and various studies using RT-qPCR have detected norovirus in a considerable proportion...
Wed, 04/24/2019 - 17:00
Hanne Dubois, Frederic Sorgeloos, Soroush T. Sarvestani, Liesbet Martens, Yvan Saeys, Jason M. Mackenzie, Mohamed Lamkanfi, Geert van Loo, Ian Goodfellow, Andy Wullaert Norovirus infection is the leading cause of food-borne gastroenteritis worldwide, being responsible for over 200,000 deaths annually. Studies with murine norovirus (MNV) showed that protective STAT1 signaling controls viral...
Wed, 04/24/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 5, Page 869-877, May 2019.
Wed, 04/24/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 5, Page 869-877, May 2019.
Sat, 04/13/2019 - 00:00
Abstract Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have...
Fri, 04/05/2019 - 01:13
Available online 3 April 2019 Author(s): Baojian Sun, Albert Bosch, Mette MyrmelAbstractDetection of viruses on berries is a challenging task, often hampered by the presence of RT-qPCR inhibiting substances from berry juice. A direct extraction method for virus detection (murine norovirus and GA phage) on frozen raspberries was previously published. We expanded (different types of berries and...
Thu, 03/28/2019 - 11:00
Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children from developing countries and presents high genetic variability. We aimed to characterize the EPEC virulence-related gene (VRG) distribution and copathogens associated with diarrhea and nutrition-related outcomes in children from the low-income Brazilian semiarid region. A cross-sectional case-control study of...
Fri, 03/22/2019 - 00:00
Abstract This study investigated the synergistic effects of combined chlorine (200, 500, 700, and 1000 ppm) and vitamin B1 (1000, 2000, and 3000 ppm) on the murine norovirus-1 (MNV-1), a human norovirus (NoV) surrogate, on oyster surface. Vitamin B1 slightly reduced MNV-1 (0.04–0.3 log-reduction), whereas chlorine significantly reduced MNV-1 (0.4–1.0 log-reduction). The combined...
Thu, 03/21/2019 - 00:00
Abstract Two outbreaks of norovirus and acute gastroenteritis took place in Canada between November 2016 and April 2017. Both outbreaks were linked to oysters from British Columbia (BC) coastal waters. This paper describes the multi-agency investigations to identify the source and control the outbreak. Public health officials conducted interviews to determine case exposures....
Wed, 03/13/2019 - 01:12
Available online 12 March 2019 Author(s): Shaofeng Rong, Yue Zhou, Ming Wang, Shimin Guan, Shuo Zhang, Baoguo Cai, Dapeng Wang, Peng Tian, Qianqian LiAbstractNorovirus is a very contagious virus that causes acute gastroenteritis. Contaminated produce is a main vehicle for dissemination of human noroviruses (HuNoVs). As HuNoVs could bind to bacteria effectively, it is highly possible that produce...
Fri, 03/01/2019 - 11:48
Abstract Human noroviruses (HNoVs) cause significant gastrointestinal disease outbreaks worldwide. Tulane virus (TV) is a cultivable HNoV surrogate widely used to determine control measures against HNoVs. The objective of this study was to determine the heat inactivation kinetics (D‐ and z‐values) of TV in cell‐culture media and on spiked homogenized spinach using the first‐order and Weibull...
Wed, 02/27/2019 - 01:12
Available online 25 February 2019 Author(s): Ningbo Liao, Liming Gong, Lili Chen, Liang Sun, Jiang Chen, Xiuyu Lou, Haiyan Mao, Yanjun Zhang, Ronghua ZhangAbstractIn China, edible shellfish are recognized as foods potentially at high risk of contamination with human Norovirus (HuNoV) and Vibrio parahaemolyticus. In this study, the antimicrobial effects of Chinese rice wine on HuNoV surrogates (...
Sat, 02/09/2019 - 00:00
Abstract A gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses...
Wed, 02/06/2019 - 01:08
Publication date: December 2018Source: Microbial Risk Analysis, Volume 10Author(s): Robyn C. Miranda, Donald W. SchaffnerAbstractFoodborne illness outbreaks have been increasingly linked to the consumption of fresh and frozen berries that were contaminated with pathogenic viruses, such as human norovirus (NoV). Contamination of berries is assumed to take place at harvest by the use of...
Wed, 02/06/2019 - 01:08
Publication date: August 2018Source: Microbial Risk Analysis, Volume 9Author(s): Mary E. Schoen, Michael A. Jahne, Jay GarlandAbstractWe assessed the annual probability of infection resulting from non-potable exposures to distributed greywater and domestic wastewater treated by an aerobic membrane bioreactor (MBR) followed by chlorination. A probabilistic quantitative microbial risk assessment...
Tue, 02/05/2019 - 01:14
Yoshiyuki Suzuki, Yen Hai Doan, Hirokazu Kimura, Hiroto Shinomiya, Komei Shirabe, Kazuhiko Katayama
Thu, 01/31/2019 - 01:11
Available online 29 January 2019 Author(s): Xuwen Gao, Ziwei Wang, Yixin Wang, Zhongmei Liu, Xueting Guan, Yingying Ma, Han Zhou, Yanping Jiang, Wen Cui, Li Wang, Yigang XuAbstractNorovirus (NoV), a major food-borne virus, causes non-bacterial acute gastroenteritis in humans. Berries are generally harvested from low-growing bushes by hand and are minimally processed before being sold to consumers...
Sun, 01/06/2019 - 01:14
Available online 4 January 2019 Author(s): Pradip Gyawali, Graham C. Fletcher, Dorothy-Jean McCoubrey, Joanne HewittAbstractFilter feeding bivalve molluscan shellfish such as oysters, mussels and clams can readily accumulate norovirus present in growing water contaminated by human faecal contamination by point and non-point sources. While pre-harvest preventative interventions are preferable,...
Sat, 12/29/2018 - 01:11
Publication date: Available online 27 December 2018 Innovative Food Science & Emerging TechnologiesAuthor(s): Radhia Abdelkebir, Cristina Alcántara, Irene Falcó, Gloria Sánchez, Jose V. Garcia-Perez, Mohamed Neffati, José M. Lorenzo, Francisco J. Barba, María Carmen ColladoAbstractErodium glaucophyllum is a common Mediterranean herb known for its health benefits. In this study, Erodium...
Sat, 12/22/2018 - 01:06
Available online 21 December 2018 Author(s): Irene Falcó, Walter Randazzo, Jesús Rodríguez-Díaz, Roberto Gozalbo-Rovira, Daniel Luque, Rosa Aznar, Gloria SánchezAbstractAged-green tea extract (GTE) is known to reduce the infectivity of hepatitis A virus (HAV) and murine norovirus (MNV), a human norovirus surrogate, in vitro and in washing solutions. Initially, the effect of aged-GTE was evaluated...
Mon, 12/17/2018 - 00:00
Abstract We investigated the present forms of genogroup II norovirus and group A rotavirus in surface water used for drinking water production. River water samples (N = 15) collected at a drinking water treatment plant (DWTP) monthly from June 2017 to August 2018 were fractioned by filtration through 10- and 0.45-μm-pore-size membranes, and viruses present in suspended and...
Thu, 12/06/2018 - 01:11
Available online 5 December 2018 Author(s): Nigel Cook, Luminita Williams, Martin D’AgostinoAbstractTo acquire data on contamination with Norovirus in berry fruit and salad vegetables in the United Kingdom, one thousand one hundred and fifty two samples of fresh produce sold at retail in the UK were analysed for Norovirus. Of 568 samples of lettuce, 30 (5.3%) were Norovirus-positive. Most (24/30...
Wed, 12/05/2018 - 01:08
Available online 21 November 2017 Author(s): J.A. Lowther, A. Bosch, S. Butot, J. Ollivier, D. Mäde, S.A. Rutjes, G. Hardouin, B. Lombard, P. in't Veld, A. LeclercqAbstractHepatitis A virus (HAV) and norovirus are important agents of food-borne human viral illness, with common vehicles including bivalve molluscan shellfish, soft fruit and various vegetables. Outbreaks of viral illness due to...
Wed, 12/05/2018 - 01:08
Available online 8 November 2018 Author(s): Licheng Huang, Xin Luo, Jingwen Gao, Karl R. MatthewsAbstractFoodborne illnesses caused by norovirus contaminated fresh produce remain a food safety concern worldwide. In the present study, the impacts of commercial and home processing conditions of strawberries were evaluated for inactivation of the MS2 bacteriophage. MS2 was used as a surrogate of...
Wed, 12/05/2018 - 01:08
Publication date: 2 February 2019 , Volume 290Author(s): Fernando Pérez-Rodríguez, Junehee Kwon, Araceli Bolívar, Kevin Sauer, Dojin Ryu, Ewen ToddAbstractHuman noroviruses (NoV) are recognized worldwide as important pathogens and the primary cause of foodborne disease outbreaks from contaminated food in the U.S. They are often transmitted by infected food handlers manipulating foods during...
Thu, 11/15/2018 - 00:00
Abstract With increasing interest in peracetic acid (PAA) as a disinfectant in water treatment processes, this study determined PAA treatment effects on human noroviruses (hNoVs) genotype I (GI) and genotype II (GII) as well as effects on bacteriophage MS2 and murine norovirus (MNV) in relation to pH. Across all pH conditions, PAA achieved between 0.2 and 2.5 log10 reduction of...
Wed, 11/14/2018 - 01:10
Available online 12 November 2018 Author(s): Hae-Won Lee, So-Ra Yoon, Hee-Min Lee, Jae Young Lee, Sung Hyun Kim, Ji-Hyoung HaAbstractIn this study, we evaluated the virucidal effects of chloroform, ethyl acetate, and n-butanol soluble fractions of ethanolic halophyte extracts, including extracts of iceplant (Mesembryantchemum crystallinum L.), salt sandspurry (Spergularia marina L. Besser), beach...
Wed, 10/17/2018 - 01:11
Available online 15 October 2018 Author(s): Melina Elizabeth Barrios, María Dolores Blanco Fernández, Robertina Viviana Cammarata, Carolina Torres, Viviana Andrea MbayedAbstractAlternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters. In this study, F-specific RNA bacteriophages (...
Thu, 10/11/2018 - 01:11
Alain Rincé, Charlotte Balière, Dominique Hervio-Heath, Joëlle Cozien, Solen Lozach, Sylvain Parnaudeau, Françoise S. Le Guyader, Simon Le Hello, Jean-Christophe Giard, Nicolas Sauvageot, Abdellah Benachour, Sofia Strubbia, Michèle Gourmelon
Wed, 09/05/2018 - 00:00
Abstract The protrusion (P) domain of the major structural protein VP1 of norovirus (NoV) is critical for the host’s immune response and receptor binding. Most heterologous P domains expressed in Escherichia coli or Komagataella pastoris (formally known as Pichia pastoris) form P particles consisting of 24 P monomers formed through intermolecular contact in the P regions and an...
Tue, 09/04/2018 - 01:12
Available online 1 September 2018 Author(s): Christina Bartsch, Carolina Plaza-Rodriguez, Eva Trojnar, Matthias Filter, Reimar JohneAbstractFrozen berries contaminated with human norovirus (hNV) have been frequently identified as cause of foodborne gastroenteritis. To prevent virus transmission, heating can be applied to the berries. Here, the thermal inactivation of hNV in strawberry puree was...

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