Research Publications (Food Safety)

This experiment aimed to explore the interaction of β-1,3-glucan and Clostridium perfringens on the growth performance, intestinal health and cecal microflora of broilers. A total of 384 one-day-old Arbor Acre broilers were sorted into 4 treatments with 6 replications. There were 2 factors in this trial: dietary β-1,3-glucan addition including 0 and 250 mg/kg, intestinal enteritis challenged with Clostridium perfringens attack or not.

In recent years, there has been an increasingly heated debate on whether Chinese dark tea is contaminated with mycotoxins and whether it poses health risks to consumers. In this study, a rapid method based on high-performance liquid chromatography was used to detect ochratoxin A (OTA) in Chinese dark tea samples from different regions of China and different years.

Food safety and health are the themes of today's society. As a class of foodborne pathogens, Salmonella enteritidis has become one of the common zoonotic pathogens. Because chemical preservatives have certain harmfulness and have been questioned, it is particularly important to find green and safe natural preservatives. The advantages of plant essential oils (EOs) are that they are green and safe, have a wide range of antibacterials, and are not easy to form drug resistance.

Biofilm-associated bacteria, especially ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), are a serious challenge worldwide. Due to the lack of discovery of novel antibiotics, in the past two decades, it has become necessary to search for new antibiotics or to study synergy with the existing antibiotics so as to counter life-threatening infections.

Zearalenone (ZEN), one of the most hazardous mycotoxins commonly present in food, causes severe safety risks to human health. In this work, the ZEN detoxification potential of dye-decolorizing peroxidase BsDyP from Bacillus subtilis 168 was investigated by a combined computational and experimental study. Molecular docking and dynamics simulation suggested that BsDyP could allow the binding of ZEN at the γ-edge of heme and surface exposed redox-active Tyr335 residue.

Cadmium (Cd) toxicity in leaves decreases their photosynthetic efficiency by degrading photosynthetic pigments, reducing the activity of gas exchange parameters and photosystem II (PSII), and producing reactive oxygen species. Although acetone O-(4-chlorophenylsulfonyl) oxime (AO) alleviates stress due to heavy metals in plants, its effects on the photosynthetic apparatus and redox balance under Cd stress are not clear.

A new green competitive ELISA for aflatoxin M1 quantification in raw milk was developed. This diagnostic tool is based on an anti AFM1 mAb produced by plant molecular farming in alternative to classical systems. Our assay, showing an IC50 below 25 ng/L, fits with the requirements of EU legislation limits for AFM1 (50 ng/L).

Feeding farm animals with aflatoxin-contaminated feed can cause various severe toxic effects, leading to increased susceptibility to infectious diseases and increased mortality, weight loss, poor performance and reduced reproductive capability. Following ingestion of contaminated foodstuffs, aflatoxins are metabolized and biotransformed differently in animals. Swine metabolism is not effective in detoxifying and excreting aflatoxins, meaning the risk of aflatoxicosis is increased.

In China, the status and risks of pesticide residues in edible mint is essentially unknown. In this study, we evaluated the residual status and associated risks of 208 pesticides in edible mints. Among the 16 pesticide residues detected, there were eight fungicides, six insecticides, and two herbicides, with difenoconazole exhibiting the highest detection rate of 25.55%. A proportion of 53.28% of the mint samples were detected with at least one pesticide residue.

This study investigated the antimicrobial and antibiofilm efficacy of separate and combined treatments of Lactobacillus curvatus B67-produced postbiotic and the polyphenolic flavanol quercetin against Listeria monocytogenes and Salmonella enterica ser. Typhimurium. The antimicrobial potentiality of the postbiotic was chiefly associated with organic acids (e.g., lactic and acetic acids).

The research on strategies to reduce cadmium (Cd) accumulation in cacao beans is currently limited by a lack of understanding of the Cd transfer pathways within the cacao tree. Here, we elucidated the transfer of Cd from soil to the nib (seed) in a high Cd accumulating cacao cultivar.

This study aimed to investigate the effects of different feeding patterns on meat quality, gut microbiota and its metabolites of Tibetan pigs. Tibetan pigs with similar body weight were fed the high energy diets (HEP, 20 pigs) and the regular diets (RFP, 20 pigs), and free-ranging Tibetan pigs (FRP, 20 pigs) were selected as the reference. After 6 weeks of experiment, meat quality indexes of semitendinosus muscle (SM) and cecal microbiota were measured.

Anisakidosis is a food-borne parasitic disease (FBPD) caused by the third-stage larvae of the family Anisakidae. Therefore, it is important to develop a simple, rapid and equipment-free detection method for anisakids in fish samples or seafood since current methods are time-consuming and require complex instruments.

Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern. Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.

The degradation of aflatoxin B1 (AFB1) and zearalenone (ZEA) is investigated using power ultrasound to identify suitable methods to reduce the mycotoxin content of corn. AFB1 and ZEA in corn are simultaneously degraded via power ultrasound; thus, this method has a significant effect on corn quality. The power intensity, solid-liquid ratio, and ultrasonic treatment modes significantly affect the degradation rates of AFB1 and ZEA.

The widespread fungal toxin Aflatoxin B1 (AFB1) is an inevitable pollutant affecting the health of humans, poultry, and livestock. Although studies indicate that AFB1 is hepatotoxic, there are few studies on AFB1-induced hepatotoxicity in sheep. Thus, this study examined how AFB1 affected sheep liver function 24 h after the animals received 1 mg/kg bw of AFB1 orally (dissolved in 20 mL, 4% v/v ethanol).

Aflatoxins (AFs) are the most detrimental mycotoxin, potentially hazardous to animals and humans. AFs in food threaten the health of consumers and cause liver cancer. Therefore, a safe, efficient, and friendly approach is attributed to the control of aflatoxicosis. Therefore, this study aimed to evaluate the impacts of Chlorella vulgaris (CLV) on hepatic aflatoxicosis, aflatoxin residues, and meat quality in quails.

Aflatoxins (AFs) are the most important toxic, mutagenic, and carcinogenic fungal toxins that routinely contaminate food and feed. While more than 20 AFs have been identified to date, aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1), G2 (AFG2), and M1 (AFM1) are the most common. Over 25 species of Aspergillus have been shown to produce AFs, with Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius being the most important and well-known AF-producing fungi.

This study was designed to evaluate the adaptive resistance of Salmonella Typhimurium under continuous sublethal selective pressure. Salmonella Typhimurium ATCC 19585 (STATCC) and S. Typhimurium CCARM 8009 (STCCARM) were sequentially cultured for 3 days at 37 °C in trypticase soy broth containing 1/2 × MICs of cefotaxime (CEF1/2), chloramphenicol (CHL1/2), gentamicin (GEN1/2), and polymyxin B (POL1/2).

Aspergillus flavus is a facultative parasite that contaminates several important food crops at both the pre- and post-harvest stages. Moreover, it is an opportunistic animal and human pathogen that causes aspergillosis diseases. A. flavus also produces the polyketide-derived carcinogenic and mutagenic secondary metabolite aflatoxin, which negatively impacts global food security and threatens human and livestock health.

Background Cadmium (Cd) levels of food crops can be elevated through management activities and geogenic factors. While emphasis is placed on reducing Cd in phosphorus (P) fertilizers, increasing evidence shows that Cd accumulation in plants is markedly influenced by arbuscular mycorrhizal fungi (AMF).

Transmission of foodborne pathogens by plants, especially shiga toxin-producing Escherichia coli, has become a public health concern for ready-to-eat products. Due to the patchy distribution of foodborne pathogens on commodities, random sampling before processing and following packaging cannot reliably exclude presence of pathogenic microbes. Robust, cost-effective, and reliable methods for monitoring pathogenic E.

The consumption of agri-foods contaminated with mycotoxins poses a serious threat to human health. In this study, we evaluated the presence of total aflatoxins (AFs) and ochratoxin A (OTA) in various agri-food products of national and imported origin in Chile that are consumed daily.

Novel artilysins by the fusions of phage lysin Lysqdvp001 (Lys) with outer membrane (OM) destabilizing peptides against Vibrio parahaemolyticus (V. parahaemolyticus) were designed and developed assisted by molecular docking. Eleven designed artilysins with predicted high affinity to lipopolysaccharide lipid-A (LPSA) of bacterial OM were selected, and eight designed artilysins after cloning and expression showed abilities to hydrolyze V.

Cooked battered products like nuggets and sausages are famous street foods in Malaysia. However, these foods lack prevalence data for the non-typhoidal Salmonella enterica serovars Enteritidis and Typhimurium (NTS) contaminations, thus leading to concern over foodborne outbreaks. Therefore, the present work aimed to examine the prevalence of antibiotic-resistant NTS in battered foods, and investigate their survival under simulated gastric fluid (SGF) and microwave heating.