Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Sun, 06/13/2021 - 00:00
The recurrent emergence of infection outbreaks associated with shellfish consumption is of extreme importance for public health. The present study investigated the potential application of phages AH-1, AH-4, and AH-5 to inactivate Aeromonas hydrophila, a causative agent of infections in humans associated with bivalve shellfish consumption. The inactivation of A. hydrophila was assessed in vitro,...
Wed, 06/02/2021 - 04:04
The increase in the prevalence of multidrug-resistant (MDR) Staphylococcus aureus with strong biofilm-forming capacity poses a serious public health concern. Endolysins derived from bacteriophages are a promising solution for antibiotic resistance problems. However, some natural staphylococcal endolysins have several shortcomings, such as low solubility and high sequence homology among domains....
Mon, 05/31/2021 - 22:07
Comprehensive Reviews in Food Science and Food Safety, EarlyView. Listeria monocytogenes continues to pose a food safety risk in ready-to-eat foods, including fresh and soft/semisoft cheeses. Despite L. monocytogenes being detected regularly along the cheese production continuum, variations in cheese style and intrinsic/extrinsic factors throughout the production process (e.g., pH, water activity...
Sun, 05/23/2021 - 06:08
Author(s): Anamaria M.P. dos Santos, Pedro Panzenhagen, Rafaela G. Ferrari, Grazielle L. Rodrigues, Carlos A. Conte-Junior Most of the Salmonella enterica pathogeny is directly related to the presence of virulence factors, which are primarily genes encoded in different parts of the chromosome (dos Santos et al., 2019). However, many of those genes can also be originated from mobile elements, as...
Sat, 05/22/2021 - 00:00
The antimicrobial resistance crisis is a Global Health challenge that impacts humans, animals, and the environment alike. In response to increased demands for animal protein and by-products, there has been a substantial increase in the use of antimicrobial agents in the animal industry. Indeed, they are extensively used to prevent, control, and (or) treat disease in animals. In addition to...
Tue, 05/18/2021 - 22:07
Journal of Food Safety, EarlyView. Bacillus cereus is among the primary food-poisoning pathogenic bacterium that causes diarrhea and emetic types of diseases throughout the world. Recent advances show that bacteriophages become important tools in detection and control of foodborne bacterial pathogens in foods. They gain the interest of researchers for the food industries mainly because they are...
Fri, 05/14/2021 - 00:00
Since the 1950s, antibiotics have been used in the field of animal husbandry for growth promotion, therapy and disease prophylaxis. It is estimated that up to 80% of the antibiotics produced by the pharmaceutical industries are used in food production. Most of the antibiotics are used as feed additives at sub-therapeutic levels to promote growth. However, studies show the indiscriminate use of...
Mon, 05/03/2021 - 16:00
The detection of fecal viral pathogens in water is hampered by their great variety and complex analysis. As traditional bacterial indicators are poor viral indicators, there is a need for alternative methods, such as the use of somatic coliphages, which have been included in water safety regulations in recent years. Some researchers have also recommended the use of reference viral pathogens such...
Fri, 04/30/2021 - 08:02
by Keiji Nakamura, Yoshitoshi Ogura, Yasuhiro Gotoh, Hayashi Tetsuya Bacteriophages (or phages) play major roles in the evolution of bacterial pathogens via horizontal gene transfer. Multiple phages are often integrated in a host chromosome as prophages, not only carrying various novel virulence-related genetic determinants into host bacteria but also providing various possibilities for prophage-...
Wed, 04/28/2021 - 12:00
ABSTRACT Salmonellosis is an important worldwide food-borne disease. Increasing resistance to Salmonella spp. has been reported in recent years, and now the prevalence of multidrug-resistant Salmonella spp. is a worldwide problem. This necessitates alternative approaches like phage therapy. This study aimed to isolate bacteriophages specific for Salmonella enterica serovar Paratyphi B and S....
Sun, 03/21/2021 - 04:02
Bacterial infections are a major threat to human health, exacerbated by increasing antibiotic resistance. These infections can result in tremendous morbidity and mortality, emphasizing the need to identify and treat pathogenic bacteria quickly and effectively. Recent developments in detection methods have focused on electrochemical, optical, and mass-based biosensors. Advances in these systems...
Fri, 03/19/2021 - 06:04
LWT
Author(s): Khashayar Shahin, Lili Zhang, Abbas Soleimani Delfan, Majid Komijani, Abolghasem Hedayatkhah, Hongduo Bao, Mohadeseh Barazandeh, Mojtaba Mansoorianfar, Maoda Pang, Tao He, Majid Bouzari, Ran Wang Shigella, the globally distributed causative agent of bacillary dysentery, is an important microbial contamination in raw and ready-to-eat foods. The recent commercial use of phage cocktails...
Thu, 03/18/2021 - 16:00
Shiga toxin is the major virulence factor of enterohemorrhagic Escherichia coli (EHEC), and the gene encoding it is carried within the genome of Shiga toxin-converting phages (Stx phages). Numerous Stx phages have been sequenced to gain a better understanding of their contribution to the virulence potential of EHEC. The Stx phages are classified into the lambdoid phage family based on...
Thu, 03/18/2021 - 00:00
Listeria monocytogenes is a bacterial foodborne pathogen and the causative agent of the disease listeriosis, which though uncommon can result in severe symptoms such as meningitis, septicemia, stillbirths, and abortions and has a high case fatality rate. This pathogen can infect humans and other animals, resulting in massive health and economic impacts in the United States and globally....
Wed, 03/10/2021 - 16:00
Vibrio parahaemolyticus is a foodborne pathogen that is frequently isolated from a variety of seafood. To control this pathogenic Vibrio spp., the implementation of bacteriophages in aquaculture and food industries have shown a promising alternative to antibiotics. In this study, six bacteriophages isolated from the seafood samples demonstrated a narrow host range specificity that infecting only...
Mon, 03/08/2021 - 16:00
Phage-based approaches have gained increasing interest as sustainable alternative strategies to antibiotic treatment or as prophylactic measures against disease outbreaks in aquaculture. The potential of three methods (oral, bath, and injection) for delivering a two-component phage mixture to rainbow trout fry for controlling Flavobacterium psychrophilum infections and reduce fish mortality was...
Mon, 03/08/2021 - 16:00
Staphylococcus aureus is an important human pathogen that can be frequently encountered in clinical and food-processing surroundings. Among the various countermeasures, bacteriophages have been considered to be promising alternatives to antibiotics. In this study, the bacteriophage PALS2 was isolated from bird feces, and the genomic and biological characteristics of this phage were investigated....
Sat, 03/06/2021 - 00:00
Preslaughter starvation and subacute ruminal acidosis in cattle are known to promote ruminal proliferation of atypical enteropathogenic Escherichia coli strains, thereby increasing the risk of meat and milk contamination. Using bacteriophages (henceforth called phages) to control these strains in the rumen is a potentially novel strategy. Therefore, this study evaluated the viability of phages...
Fri, 03/05/2021 - 06:07
Author(s): Haiyan Zeng, Chengsi Li, Dandan Luo, Jumei Zhang, Yu Ding, Moutong Chen, Xiaojuan Yang, Tao Lei, Shi Wu, Qinghua Ye, Rui Pang, Qihui Gu, Qingping Wu Cronobacter spp. are important foodborne pathogens that are a threat to people of all ages, but especially neonates and infants. Bacteriophages are biological agents that are potentially useful for the control of foodborne pathogens....
Wed, 03/03/2021 - 10:02
ABSTRACT Neonatal calf diarrhea is a common disease leading to a major economic loss for cattle producers worldwide. Several infectious and noninfectious factors are implicated in calf diarrhea, but disease control remains problematic because of the multifactorial etiology of the disease. Here, we conducted diagnostic multiplex PCR assay and meta-omics analysis (16S rRNA gene-based metataxonomics...
Thu, 02/25/2021 - 16:00
Shiga toxin (Stx), encoded by stx genes located in prophage sequences, is the major agent responsible for the pathogenicity of Shiga toxin-producing Escherichia coli (STEC) and is closely associated with the development of hemolytic uremic syndrome (HUS). Although numerous Stx prophage sequences have been reported as part of STEC bacterial genomes, the information about the genomic...
Wed, 02/24/2021 - 16:00
Effectiveness of bacteriophages AKFV33 (Tequintavirus, T5) and AHP24 (Rogunavirus, T1), wV7 (Tequatrovirus, T4), and AHP24S (Vequintavirus, rV5), as well as 11 cocktails of combinations of the four phages, were evaluated in vitro for biocontrol of six common phage types of Escherichia coli O157 (human and bovine origins) at different multiplicities of infection (MOIs; 0.01–1,000), temperatures (...
Wed, 02/17/2021 - 16:00
Bacteriophages, viruses that infect and replicate within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the Enterobacteriaceae family, with Escherichia coli being the major subject of studies, have been isolated from wastewater, sewage, and...
Thu, 02/11/2021 - 02:01
The objective of this study was to use high-energy electron beam (HEEB) treatments to find surrogate microorganisms for enteric viruses and to use the selected surrogates as proof of concept to investigate low-energy electron beam (LEEB) treatments for enteric virus inactivation at industrial scale on frozen blueberries. Six food matrices inoculated with HAV (hepatitis A virus), MNV S99 (murine...
Sun, 02/07/2021 - 02:01
Oysters contaminated with human enteric viruses from sewage are implicated in foodborne outbreaks globally. Bacteriophages have been identified as potential indicators for these viruses, but have not been used in shellfish management outside of the USA. This study aimed to determine the background levels of F-RNA phage in five Australian oyster growing areas with a history of sewage spills and...
Mon, 01/25/2021 - 16:00
Citrobacter species often occur in sewage, food, soil, wastewater, and in the intestinal tract of animals and humans. Citrobacter spp. cause urinary tract infections (UTIs) and infantile meningitis in humans. Due to the presence of plasmid-encoded resistance genes, Citrobacter spp. are often resistant to many antibiotics. In this study, Citrobacter virus HCF1, a novel virulent bacteriophage...
Sun, 01/24/2021 - 02:01
Unraveling the trends of phage-host versus phage-phage coevolution is critical for avoiding possible undesirable outcomes from the use of phage preparations intended for therapeutic, food safety or environmental safety purposes. We aimed to investigate a phenomenon of intergeneric recombination and its trajectories across the natural populations of phages predominantly linked to foodborne...
Wed, 01/20/2021 - 16:00
Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens with Shiga toxins as the main virulence factor. Shiga toxins are encoded on Shiga toxin-encoding bacteriophages (Stx phages). Stx phages may exist as free bacteriophages in the environment or in foods or as prophages integrated into the host genome. From a food safety perspective, it is important to have knowledge on...
Wed, 01/20/2021 - 06:06
Author(s): Dandan Luo, Chengsi Li, Qingping Wu, Yu Ding, Meiyan Yang, Yongdan Hu, Haiyan Zeng, Jumei Zhang Cronobacter spp. are opportunistic pathogenic bacteria that cause severe diseases in neonates and infants. Bacteriophages are novel antibacterial agents with a potential to control this pathogen. In the current study, a novel lytic Cronobacter phage, vB_CtuP_A24, was isolated from a river in...
Mon, 01/11/2021 - 16:00
Leuconostoc carnosum is a known colonizer of meat-related food matrices. It reaches remarkably high loads during the shelf life in packaged meat products and plays a role in spoilage, although preservative effects have been proposed for some strains. In this study, the draft genomes of 17 strains of L. carnosum (i.e., all the strains that have been sequenced so far) were compared to decipher...
Thu, 01/07/2021 - 10:02
Enterohemorrhagic Escherichia coli (EHEC) isolates of serotype O157:H7 are serious foodborne zoonotic pathogens and prime targets for biocontrol using bacteriophages. We report on the complete genome sequences of 11 novel lytic bacteriophages, representing three viral genera, isolated from cattle in Hungary that target E. coli O157 strains.
Thu, 01/07/2021 - 10:02
Bacteriophages can be used as a biocontrol for the foodborne pathogen Listeria monocytogenes. Propagation of phages is a necessary step for their use in experimental studies and biocontrol applications. Here, we present the complete genomes of three Listeria monocytogenes strains commonly used as propagation hosts for Listeria phages.
Fri, 12/25/2020 - 06:06
Author(s): Junjie Li, Yameng Li, Yifeng Ding, Chenxi Huang, Yu Zhang, Jia Wang, Xiaohong Wang Salmonella is one of the most common causes of foodborne diseases and can cause severe economic loss. Increased antibiotic resistance, particularly of multidrug-resistant strains, has led to the use of phages as substitute agents. However, bacteriophages are usually sensitive to harsh environments. At...
Sat, 12/12/2020 - 06:04
Author(s): E.L. Shebs-Maurine, F.M. Giotto, S.T. Laidler, A.S. de Mello Research has suggested that the incidence of Salmonella in ground beef may be associated with contaminated lymph nodes that are not removed from trimmings destined for grinding. In this study, we tested the application of bacteriophages and peroxyacetic acid solutions on trimmings and on coarse and fine ground beef to...
Thu, 12/03/2020 - 22:09
The application of bacteriophage P100 to reduce incidence of Listeria sp. in ready‐to‐eat (RTE) food production environments was investigated. Data indicate the application of Listeria specific phage P100 in RTE food production environments can reduce incidence and be considered an additional intervention strategy for controlling this pathogen on NFCS. Abstract Listeria monocytogenes is a...
Thu, 12/03/2020 - 16:00
Acinetobacter baumannii is of major clinical importance as the bacterial pathogen often causes hospital acquired infections, further complicated by the high prevalence of antibiotic resistant strains. Aside from natural tolerance to certain antibiotic classes, resistance is often acquired by the exchange of genetic information via conjugation but also by the high natural competence exhibited by A...
Wed, 11/25/2020 - 08:02
by Anna V. Skorynina, Emma G. Piligrimova, Olesya A. Kazantseva, Vladislav A. Kulyabin, Svetlana D. Baicher, Natalya A. Ryabova, Andrey M. Shadrin Several bacterial species belonging to the Bacillus cereus group are known to be causative agents of food poisoning and severe human diseases. Bacteriophages and their lytic enzymes called endolysins have been widely shown to provide for a supplemental...
Wed, 11/11/2020 - 16:00
Rifampin plays a crucial role in the treatment of staphylococcal implant-associated infection, as it is the only antibiotic capable of eradicating Staphylococcus aureus biofilms. However, the emergence of rifampin resistance strongly limits its use. Combinatorial therapy of antibiotics and bacteriophages may represent a strategy to overcome the resistance. Here, we evaluated the activity of...
Sun, 11/08/2020 - 22:13
Salmonellosis is one of the most important bacterial diseases in pigeons. This study aimed to estimate the prevalence of Salmonella spp. in domestic pigeons (Columba livia f. domestica) in Poland, its antimicrobial susceptibility (both phenotypic and genotypic), and its capability for biofilm formation. The presence of selected virulence genes, nucleotide homology of selected genes, and...
Sat, 11/07/2020 - 06:16
Author(s): Jae-Hyun Cho, Joon-Gi Kwon, Daniel J. O'Sullivan, Sangryeol Ryu, Ju-Hoon Lee Clostridium perfringens is a well-known pathogen that causes food-borne illnesses. Although bacteriophages can be effective natural food preservatives, phage endolysin and cell wall–binding domain (CBD) provide useful materials for lysis of C. perfringens and rapid detection. The genome of phage CPAS-15...
Thu, 10/29/2020 - 10:02
Bacteriophages (phages) are currently available for use by the food industry to control the foodborne pathogen Listeria monocytogenes. Although phage biocontrols are effective under specific conditions, their use can select for phage-resistant bacteria that repopulate phage-treated environments. Here, we performed short-term coevolution experiments to investigate the impact of single phages and a...
Thu, 10/29/2020 - 06:15
LWT
Author(s): Özgül Aydin Demirarslan, Hamza Alasalvar, Zeliha Yildirim The present study was conducted to investigate the virulent effects of SE-P3, P16, P37, and P47 phages specific to Salmonella Enteritidis, alone or as a cocktail on raw chicken meat and chicken skin. Chicken meat and skin artificially contaminated with 103 and 106 log CFU/g or cm2 Salmonella were treated with SE-phages at a...
Thu, 10/22/2020 - 10:02
ABSTRACT Supply shortages of N95 respirators during the coronavirus disease 2019 (COVID-19) pandemic have motivated institutions to develop feasible and effective N95 respirator reuse strategies. In particular, heat decontamination is a treatment method that scales well and can be implemented in settings with variable or limited resources. Prior studies using multiple inactivation methods,...
Tue, 10/20/2020 - 22:12
The research showed that ruminant animal is the primary factor contributing to the prevalence of the STEC‐infecting phages on the organic farm in this study. Among environmental factors, high rain precipitation is likely to facilitate phage distribution in the surrounding environment of the farm. Most importantly, the presence of these phages has a negative correlation with their STEC hosts....
Thu, 10/15/2020 - 06:11
Author(s): Dongryeoul Bae, Jeong-Woo Lee, Jong-Pyo Chae, Jae-Won Kim, Jong-Su Eun, Kyung-Woo Lee, Kun-Ho Seo High necrotic enteritis (NE) incidence and mortality rates in poultry can be caused by Clostridium perfringens (CP) co-infected with Eimeria spp., a causative agent of coccidiosis. Banning of prophylactic use of antibiotics in feed has been accompanied by increased NE outbreaks, resulting...
Fri, 10/09/2020 - 16:00
Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC). These toxigenic bacteriophage hosts can cause life-threatening illnesses, and Stx is the virulence determinant responsible for the severe nature of infection with enterohemorrhagic E....
Wed, 10/07/2020 - 08:02
Enterohemorrhagic Escherichia coli is a significant human pathogen that causes disease ranging from hemorrhagic colitis to hemolytic uremic syndrome. The latter can lead to potentially fatal renal failure and is caused by the release of Shiga toxins that are encoded within lambdoid bacteriophages. The toxins are encoded within the late...
Fri, 10/02/2020 - 10:01
Shiga toxin-producing Escherichia coli (STEC) is a leading cause of foodborne infections. Cattle are an important STEC reservoir, although little is known about specific pathogen traits that impact persistence in the farm environment. Hence, we sought to evaluate STEC isolates recovered from beef cattle in a single herd in Michigan. To do this, we collected fecal grabs from 26 cattle and...
Thu, 10/01/2020 - 10:07
The use of bacteriophages as antimicrobial agents represents a promising alternative for the control of pathogenic bacteria. Here, we present the complete genome sequences of two novel Salmonella enterica lytic bacteriophages, NBSal006 and NBSal007, candidates for Salmonella biocontrol.
Thu, 09/24/2020 - 16:00
Bacteriophages use a large number of different bacterial cell envelope structures as receptors for surface attachment. As a consequence, bacterial surfaces represent a major control point for the defense against phage attack. One strategy for phage population control is the production of outer membrane vesicles (OMVs). In Gram-negative host bacteria, O-antigen-specific bacteriophages address...

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