Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Fri, 06/18/2021 - 04:02
DNA-based detection methods are highly promising for risk assessment in the food sector, such as tracing the existence of food allergens. However, due to the complexity of food matrices, cumbersome protocols are often needed to isolate the DNA components, which hinder the achievement of rapid and on-site detection. Herein, an instant and multiple DNA extraction method was developed based on the...
Thu, 06/10/2021 - 02:01
The long-term accumulation of pesticide residues brought a huge threat to healthy lives of animals and humans. However, traditional detection methods for pesticide residues required expensive equipment and tedious operation. An electrochemiluminescence (ECL) aptasensor based on nano-antimony tin oxide and titanium dioxide composites (ATO@TiO2) and silver nanoparticles (AgNPs) for rapid detection...
Sun, 06/06/2021 - 00:00
The first part of this study evaluates the occurrence of mycotoxin patterns in feedstuffs and fish feeds. Results were extrapolated from a large data pool derived from wheat (n = 857), corn (n = 725), soybean meal (n = 139) and fish feed (n = 44) samples in European countries and based on sample analyses by liquid chromatography/tandem mass spectrometry (LC-MS/MS) in the period between 2012–2019...
Mon, 05/31/2021 - 00:00
Vibrio parahaemolyticus and Aeromonas hydrophila are major public health problems and the main cause of bacterial disease in Nile tilapia (Oreochromis niloticus). This study was conducted to determine the prevalence, antibiotic resistance and some virulence genes of both V. parahaemolyticus and A. hydrophila isolates from Nile tilapia. From Manzala Farm at Dakahlia governorate, 250 freshwater...
Fri, 05/28/2021 - 06:08
Author(s): Lauren Shusterman, Antoinette E. Marsh, Priscilla H. Joyner, Greg Habing Trichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institutions have used...
Wed, 05/12/2021 - 22:06
Comprehensive Reviews in Food Science and Food Safety, Volume 20, Issue 3, Page 2332-2381, May 2021. Aflatoxins represent a global public health and economic concern as they are responsible for significant adverse health and economic issues affecting consumers and farmers worldwide. Produced by fungal species from the Aspergillus genus, aflatoxins are a toxic, mutagenic, and carcinogenic group of...
Tue, 05/04/2021 - 06:10
Author(s): Alka Rani, Vivek B. Ravindran, Aravind Surapaneni, Nitin Mantri, Andrew S. Ball Escherichia coli O157:H7, a Shiga-producing E. coli is a major pathogenic E. coli strain which since the early 1980's has become a crucial food and water-borne pathogen. Several management strategies can be applied to control the spread of infection; however early diagnosis represents the optimum preventive...
Fri, 04/23/2021 - 12:00
ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (...
Wed, 04/21/2021 - 04:02
In this study, we compared the efficiency of culture-based methods with or without membrane filtration, real-time PCR and digital droplet PCR (ddPCR) for the detection of Campylobacter in fresh produce. Alfalfa sprouts, clover sprouts, coleslaw, and lettuce salad spiked with Campylobacter jejuni ( C. jejuni ) were enriched in Bolton broth for 48 h and enrichment cultures were either directly...
Mon, 04/12/2021 - 16:00
BackgroundUrinary tract infections (UTIs) are one the most common infections. The rapid and accurate identification of uropathogens, and the determination of antimicrobial susceptibility, are essential aspects of the management of UTIs. However, existing detection methods are associated with certain limitations. In this study, a new urinary tract infection high-throughput multiplex genetic...
Tue, 04/06/2021 - 02:01
Biogenic amines (BAs) are organic nitrogenous compounds, formed mostly by decarboxylation of corresponding amino acids. BAs are responsible for several biological events. However, if the concentration of BAs reached the threshold level, it causes mild to serious health problems in human. The objective of this manuscript was to summarize the prevalence and prevention of Bas formation, detection...
Sun, 03/21/2021 - 04:02
Bacterial infections are a major threat to human health, exacerbated by increasing antibiotic resistance. These infections can result in tremendous morbidity and mortality, emphasizing the need to identify and treat pathogenic bacteria quickly and effectively. Recent developments in detection methods have focused on electrochemical, optical, and mass-based biosensors. Advances in these systems...
Thu, 03/18/2021 - 22:05
Comprehensive Reviews in Food Science and Food Safety, EarlyView. Phenolic compounds are commonly incorporated into muscle foods to inhibit lipid oxidation and modify product flavor. Those that are present in or extracted from plant sources (seeds, leaves, and stems) known as “phytophenols” are of particular importance in the current meat industry due to natural origins, diversity, and safety...
Wed, 03/10/2021 - 06:06
Author(s): Antonia Kreitlow, André Becker, Ulrich Schotte, Burkhard Malorny, Madeleine Plötz, Amir Abdulmawjood Salmonella infection represents a common foodborne zoonosis of public concern worldwide. Rapid and user-friendly nucleic acid-based detection methods like loop-mediated isothermal amplification (LAMP) can contribute to the improvement of food safety and prevention of foodborne illness....
Fri, 03/05/2021 - 06:07
Author(s): Xiaoying Han, Yuanhui Liu, Juxin Yin, Min Yue, Ying Mu The global burden of foodborne diseases is substantial and foodborne pathogens are the major cause for human illnesses. In order to prevent the spread of foodborne pathogens, detection methods are constantly being updated towards rapid, portable, inexpensive, and multiplexed on-site detection. Due to the nature of the small size...
Thu, 03/04/2021 - 08:04
The presence of Cronobacter sakazakii must be controlled in infant powder plants, because it may cause infectious disease in infants, with high mortality. Testing for C. sakazakii in powdered infant formula should be performed before delivery, and it requires rapid and specific detection methods. In this study, we established a surface-enhanced Raman scattering (SERS) immunochromatographic test...
Tue, 03/02/2021 - 08:04
Due to the lack of specific genes for rapid detection methods of Cronobacter sakazakii in food samples, whole genome sequence analysis was performed in this investigation using the basic local alignment search tool. Forty-two DNA fragments unique to C. sakazakii were mined, then primers were designed and screened by PCR and loop-mediated isothermal amplification (LAMP). Two primer sets, CS1 and...
Tue, 03/02/2021 - 04:01
Pesticide residues are a food safety concern. A good detection method is critical for rapid and accurate determination of pesticide metabolites in crops and studying metabolism. The pretreatment methods have mainly been ultrasonic extraction–solid-phase extraction and QuEChERS, while detection methods have been radio-chromatography, nuclear magnetic resonance, and mass spectrometry. This...
Tue, 02/23/2021 - 16:00
The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed...
Fri, 02/19/2021 - 10:01
Multiple rapid antigen (Ag) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have recently received emergency-use authorization (EUA) from the U.S. Food and Drug Administration (FDA). Although less sensitive than molecular detection methods, rapid antigen testing offers the potential for inexpensive, quick, decentralized testing. Robust analytical sensitivity data in...
Tue, 02/16/2021 - 22:07
Comprehensive Reviews in Food Science and Food Safety, EarlyView. Understanding fruit quality is an effective way to reduce fruit loss and waste by providing proper strategies for fruit processing and management. Various noninvasive technologies have been developed for assessing fruit quality. Among them, acoustic vibration technology has received considerable attention from academics. As the...
Sat, 02/13/2021 - 10:01
Currently, only 5 (SEA to SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits. Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc), which is part of the Staphylococcus aureus genomic island vSaβ. These enterotoxins have been described as likely being...
Fri, 02/05/2021 - 02:01
The antimicrobial applications of copper (Cu) are exploited in several industries, such as agriculture and healthcare settings. While Cu is capable of efficiently killing microorganisms, sub-lethal doses can induce a viable-but-non-culturable (VBNC) state in bacteria of many distinct clades. VBNC cells cannot be detected by standard culture-based detection methods, and can become a threat to...
Sat, 01/30/2021 - 10:02
Effective and accurate primer design is an increasingly important skill, as the use of PCR-based diagnostics in clinical and environmental settings is on the rise. While universal primer sets have been successfully designed for highly conserved core genes, such as 16S rRNA, and characteristic genes, such as dsrAB and dnaJ, primer sets for mobile, accessory genes such as multidrug resistance...
Sat, 01/09/2021 - 00:00
Novel optical waveguide lightmode spectroscopy (OWLS)-based immunosensor formats were developed for label-free detection of Fusarium mycotoxin zearalenone (ZON). To achieve low limits of detection (LODs), both immobilised antibody-based (direct) and immobilised antigen-based (competitive) assay setups were applied. Immunoreagents were immobilised on epoxy-, amino-, and carboxyl-functionalised...
Wed, 12/16/2020 - 04:01
Vegetables in Cambodia are commonly sold in informal markets lacking food safety standards and controls. Currently, data on microbial contamination of vegetables in Cambodian informal markets are limited. The purpose of this study was to investigate Salmonella enterica and indicator organisms (generic Escherichia coli and coliforms) on the surface of fresh vegetables sold in informal markets in...
Tue, 11/24/2020 - 22:10
The incidence of foodborne diseases has continuingly increased over the years and resulted in public health problem globally. Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) is a human pathogen that causes diarrhea and hemorrhagic colitis. E. coli O157:H7 can be found in various foods. It is important to detect this foodborne pathogen to provide safe food supply. A lot of methods,...
Fri, 11/20/2020 - 16:00
The enterohemorrhagic Escherichia coli (EHEC) group is responsible for outbreaks and sporadic cases around the world annually. EHEC produces a potent protein known as Shiga toxin in the human intestine causing mild to bloody diarrhea. Some cases of EHEC infections may develop life-threatening symptoms, which may lead to human death. It also has other virulent factors that enable the EHEC cells to...
Fri, 11/20/2020 - 16:00
Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their...
Fri, 11/20/2020 - 00:00
Clostridium difficile colitis is caused by a cytotoxin produced by the anaerobic bacteria C. difficile in the epithelial cells of the large intestine, particularly C. difficile toxin B (TcdB). However, the sensitivity of currently utilized C. difficile endotoxin determination methods has been called into question, and, therefore, more accurate and convenient detection methods are needed. Our...
Thu, 11/12/2020 - 22:10
Salmonella is one of the main causes of foodborne infectious diseases, posing a serious threat to public health. It can enter the food supply chain at various stages of production, processing, distribution, and marketing. High prevalence of Salmonella necessitates efficient and effective approaches for its identification, detection, and monitoring at an early stage. Because conventional methods...
Sat, 11/07/2020 - 06:18
Author(s): Mohamed A. Farag, Matta A. Mesak, Doaa B. Saied, Nada M. Ezzelarab Spores are dormant structures that are widespread in various foodstuffs leading to food spoilage or foodborne illness. Many attempts have been made to eliminate and minimize spores in food with though inactivation processes not always efficient. To overcome the limitations of inactivation procedures, detection methods...
Fri, 11/06/2020 - 16:00
As an important foodborne pathogen, Vibrio vulnificus gives a significant threat to food safety and public health. Rapid and accurate detection methods for V. vulnificus are required to control its spread. The conventional detection methods are time-consuming and labor-intensive, while the polymerase chain reaction (PCR)- and quantitative PCR (qPCR)-based methods are limited because of their...
Fri, 10/30/2020 - 00:01
Ochratoxin A (OTA) is a known food contaminant that affects a wide range of food and agricultural products. The presence of this fungal metabolite in foods poses a threat to human health. Therefore, various detection and quantification methods have been developed to determine its presence in foods. Herein, we describe a rapid and ultrasensitive tracer-based fluorescence polarization immunoassay (...
Wed, 10/28/2020 - 22:11
Rapidity, sensitivity, and selectivity of the methods have been compared. Regulations of detection methods have been covered. Limitations and further studies have been suggested. Abstract The rapid, sensitive, and selective detection of foodborne pathogens is important to ensure food safety. Culture medium‐based methods for bacteria detection have long been used since Robert Koch's first finding...
Tue, 10/20/2020 - 22:10
The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment...
Sat, 10/17/2020 - 06:17
Author(s): Xiaohan Yang, Panpan Zhao, Yu Dong, Shiqi Chen, Hui Shen, Ge Jiang, Hai Zhu, Jingquan Dong, Song Gao Vibrio vulnificus is an important foodborne pathogenic bacterium that mainly contaminates seafood. Rapid and accurate technologies that suitable for on-site detection are critical for effective control of its spreading. Conventional detection methods and polymerase chain reaction (PCR)-...
Sat, 10/17/2020 - 04:02
Carbendazim (CBZ), which is a fungicide widely used for the management of plant diseases, has been detected in a number of food products. The negative effects of CBZ to human health have stimulated the reduction of the maximum residue limits (MRLs), and subsequently the development of reliable and sensitive detection methods. Here, we are reviewing for the first time all reported chromatographic...
Sat, 10/17/2020 - 00:00
The surveillance of antimicrobial resistance among humans and food-producing animals is important to monitor the zoonotic transmission of multidrug-resistant bacteria (MDRB). We assessed the prevalence of four MDRB within the meat production chain, including extended-spectrum β-lactamase (ESBL)-producing, carbapenemase-producing Enterobacterales (CPE) and colistin-resistant...
Thu, 10/15/2020 - 06:15
Author(s): Li Wang, Xingyi Huang, Chengquan Wang, Xiaoyu Tian, Xianhui Chang, Yi Ren, Shanshan Yu Food safety has always been an issue of great concern to people. The development of rapid, sensitive and specific detection technology of food pollutants is one of the hot issues in food science field. The rapid development of functionalized Fe3O4 nanoparticles (NPs) provides unprecedented...
Fri, 09/25/2020 - 08:02
by Dongxian Li, Yanyan Zhang, Qingqian Guo, Xiaoquan Sun, Hao Zhang, Shun Wang, Zephania Birech, Jiandong Hu In recent years, there has been growing concern among consumers about pesticide contamination in fruits. Therefore, rapid, reliable, and consistent detection methods for OPPs, especially dimethoate, are crucially needed. The existing quantitative methods for detecting dimethoate are not...
Wed, 09/23/2020 - 10:02
Campylobacter jejuni is a leading cause of enteric bacterial illness in the United States. Traditional molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE) and 7-gene multilocus sequence typing (MLST), provided limited resolution to adequately identify C. jejuni outbreaks and separate out sporadic isolates during outbreak investigations. Whole-genome sequencing (WGS) has...
Wed, 09/09/2020 - 14:04
The frequent occurrence of food safety incidents has given rise to unprecedented concern about food contamination issues for both consumers and the industry. Various contaminations in food pose serious threats to food safety and human health. Many detection methods were studied to address the challenge. Recently, biosensors relying on deoxyribonucleic acid (DNA)-functionalized nanoparticles have...
Wed, 09/09/2020 - 02:02
The purpose of this study was to investigate the association among polycyclic aromatic hydrocarbons (PAHs) exposure and air pollutants and the diversity of microbiota. Daily average concentrations of six common air pollutants were obtained from China National Environmental Monitoring Centre. The PAHs exposure levels were evaluated by external and internal exposure detection methods, including...
Tue, 09/08/2020 - 22:11
Rapid detection of foodborne pathogens, spoilage microbes, and other biological contaminants in complex food matrices is essential to maintain food quality and ensure consumer safety. Traditional methods involve culturing microbes using a range of nonselective and selective enrichment methods, followed by biochemical confirmation among others. The time‐to‐detection is a key limitation when...
Sat, 09/05/2020 - 22:15
The research aimed to generate an early warning system highlighting in real‐time bacterial contamination of meat matrices and providing information which could support companies in accepting or rejecting batches. Current microorganisms’ detection methods rely on techniques (plate counting), which provide retrospective values for microbial contamination. The purpose of this research was to...
Sat, 09/05/2020 - 14:04
Foodborne diseases caused by pathogens and toxins are a serious threat to food safety and human health; thus, they are major concern to society. Existing conventional foodborne pathogen or toxin detection methods, including microbiological assay, nucleic acid-based assays, immunological assays, and instrumental analytical method, are time-consuming, labor-intensive and expensive. Because of the...
Fri, 08/21/2020 - 16:00
The processing and storage conditions of flour food inevitably pose environmental stress, which promote bacteria to enter a viable but non-culturable (VBNC) state. The existence of VBNC cells causes false-negative detection in traditional culture-based detection methods, resulting in food quality and safety issues. This study aimed at investigating the influence factors including nutrition, acid...
Fri, 08/14/2020 - 06:07
Author(s): Yukiko Hara-Kudo, Kayoko Ohtsuka, Noriko Konishi, Takako Yoshida, Kaori Iwabuchi, Takahiro Hiratsuka, Yuhki Nagai, Keiko Kimata, Hiroyuki Wada, Takumiko Yamazaki, Akihiko Tsuchiya, Tetsuya Mori, Shunichi Inagaki, Shogo Shiraishi, Jun Terajima Enterotoxigenic Escherichia coli (ETEC) causes acute diarrhea and is transmitted through contaminated food and water; however, systematic...
Tue, 08/11/2020 - 06:14
Author(s): Dayna M. Harhay, Margaret D. Weinroth, James L. Bono, Gregory P. Harhay, Joseph M. Bosilevac Little progress has been made in decreasing the incidence rate of salmonellosis in the US over the past decade. Mitigating the contribution of contaminated raw meat to the salmonellosis incidence rate requires rapid methods for quantifying Salmonella, so that highly contaminated products can be...

Pages