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Research Publications from Peer-Reviewed Journals

Thu, 08/20/2015 - 01:27
The control of multihost pathogens, such as Coxiella burnetii, should rely on accurate information about the roles played by the main hosts. We aimed to determine the involvement of the red deer (Cervus elaphus) in the ecology of C. burnetii. We predicted that red deer populations from broad geographic areas within a European context would be exposed to C. burnetii, and therefore, we hypothesized...
Thu, 08/20/2015 - 01:27
Caleosins are a small family of calcium-binding proteins endowed with peroxygenase activity in plants. Caleosin-like genes are present in fungi; however, their functions have not been reported yet. In this work, we identify a plant caleosin-like protein in Aspergillus flavus that is highly expressed during the early stages of spore germination. A recombinant purified 32-kDa caleosin-like protein...
Thu, 08/20/2015 - 01:27
Phytophagous insects can encounter Salmonella enterica on contaminated plant surfaces and transmit externally adhered and internalized bacteria on and among leaves. Excretion of ingested S. enterica by the leafhopper Macrosteles quadrilineatus has been previously reported; however, the sites of persistence of ingested bacteria remain undetermined. Fluorescence microscopy revealed the presence and...
Thu, 08/20/2015 - 01:27
Publication date: Available online 20 August 2015 Author(s): Wilson J. Bertu, Marie J. Ducrotoy, Pilar M. Muñoz, Virginie Mick, Amaia Zúñiga-Ripa, Ward Bryssinckx, Jacob K.P. Kwaga, Junaid Kabir, Susan C. Welburn, Ignacio Moriyón, Reuben A. Ocholi Brucellosis is a worldwide widespread zoonosis caused by bacteria of the genus Brucella . Control of this disease in a given...
Wed, 08/19/2015 - 01:21
Foodborne Pathogens and Disease , Vol. 0, No. 0.
Wed, 08/19/2015 - 01:21
A quantitative comparison between discriminatory indexes and concordance among multilocus variable-number tandem-repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), automated ribotyping, and phage typing has been performed, testing 238 Salmonella enterica serotype Enteritidis isolates not epidemiologically correlated. The results show that MLVA is the best choice, but each typing...
Wed, 08/19/2015 - 01:21
We isolated Shiga toxin-producing Escherichia coli O157:H7 strains resistant to third-generation cephalosporins. The resistant strains harbored blaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the investigation and surveillance of enterobacteria with plasmids harboring blaCMY-...
Wed, 08/19/2015 - 01:21
The estuarine gram-negative rod and human diarrheal pathogen Vibrio cholerae synthesizes a VPS exopolysaccharide-dependent biofilm matrix that allows it to form a 3D structure on surfaces. Proteins associated with the matrix include, RbmA, RbmC, and Bap1. RbmA, a protein whose crystallographic structure suggests two binding surfaces, associates with cells...
Wed, 08/19/2015 - 01:21
Natural transformation is one mechanism of horizontal gene transfer (HGT) in Vibrio cholerae, the causative agent of cholera. Recently, it was found that V. cholerae isolates from the Haiti outbreak were poorly transformed by this mechanism. Here, we show that an integrating conjugative element (ICE)-encoded DNase, which we name...
Wed, 08/19/2015 - 01:21
H. M. Abdallah, E. A. Reuland, B. B. Wintermans, N. al Naiemi, A. Koek, A. M. Abdelwahab, A. M. Ammar, A. A. Mohamed, C. M. J. E. Vandenbroucke-Grauls Objectives The aim of the present study was to determine the prevalence and to characterize extended-spectrum β-lactamases- and/or carbapenemases-producing Enterobacteriaceae among Enterobacteriaceae isolated from retail chicken meat in Zagazig,...
Wed, 08/19/2015 - 01:21
Kirk S. B. Bergstrom, Vijay Morampudi, Justin M. Chan, Ganive Bhinder, Jennifer Lau, Hyungjun Yang, Caixia Ma, Tina Huang, Natasha Ryz, Ho Pan Sham, Maryam Zarepour, Colby Zaph, David Artis, Meera Nair, Bruce A. Vallance Enterohemorrhagic Escherichia coli and related food and waterborne pathogens pose significant threats to human health. These attaching/effacing microbes infect the apical...
Wed, 08/19/2015 - 01:21
Aims Ileocaecal lymph nodes (ICLNs) of pigs, the key immune inductive site for bacterial systemic invasion, were examined in this study with emphasis on viable and cultivable bacteria. Methods and Results Asymptomatic and pathologically altered ICLNs of slaughter pigs (n = 16) with hyperplasia, purulence or granulomatous formations were aerobically and anaerobically cultivated. In total, 209...
Wed, 08/19/2015 - 01:21
Whole-genome sequencing (WGS) has emerged today as an ultimate typing tool to characterize Listeria monocytogenes outbreaks. However, data analysis and interlaboratory comparability of WGS data are still challenging for most public health laboratories. Therefore, we have developed and evaluated a new L. monocytogenes typing scheme based on genome-wide gene-by-gene comparisons (core genome...
Wed, 08/19/2015 - 01:21
Infant botulism is a potentially life-threatening paralytic disease that can be associated with prolonged morbidity if not rapidly diagnosed and treated. Four infants were diagnosed and treated for infant botulism in NSW, Australia, between May 2011 and August 2013. Despite the temporal relationship between the cases, there was no close geographical clustering or other epidemiological links....
Wed, 08/19/2015 - 01:21
Zearalenone and its metabolites, α-zearalenol and β-zearalenol, demonstrate estradiol-like activity and disrupt physiological functions in animals. This article evaluates the carryover of zearalenone and its selected metabolites from the digesta to intestinal walls (along the entire intestines) in pre-pubertal gilts exposed to low doses of zearalenone over long periods of time. The term “...
Tue, 08/18/2015 - 20:00
Abstract Contamination concerns in the dairy industry are motivated by outbreaks of disease in humans and the inability of thermal processes to eliminate bacteria completely in processed products. HACCP principles are an important tool used in the food industry to identify and control potential food safety hazards in order to meet customer demands and regulatory requirements....
Tue, 08/18/2015 - 01:33
Bo Cui, Lei Feng, Zhenzhong Pan, Manli Yu, Zhanghua Zeng, Changjiao Sun, Xiang Zhao, Yan Wang, Haixin Cui Pesticides are essential agrochemicals used to protect plants from diseases, pests and weeds. However, the formulation defects of conventional pesticides cause food toxicity and ecological environmental problems. In this study, a novel, efficient and environmentally friendly formulation of...
Tue, 08/18/2015 - 01:33
Marua Prevato, Ilaria Ferlenghi, Alessandra Bonci, Yasushi Uematsu, Giulia Anselmi, Fabiola Giusti, Sylvie Bertholet, Francois Legay, John Laird Telford, Ethan C. Settembre, Domenico Maione, Roberta Cozzi Developing a universal influenza vaccine that induces broad spectrum and longer-term immunity has become an important potentially achievable target in influenza vaccine research and development...
Tue, 08/18/2015 - 01:33
This study was conducted to evaluate the effects of a blend of organic acids (OAs) in diets with or without antibiotic growth promoter (AGP) in chickens challenged with Clostridium perfringens. Day-old male broiler chicks were used in a trial with 4 treatments and 6 replicates of 50 birds per pen, for 43 days, in a completely randomized design. The treatments in a 2x2 factorial arrangement...
Tue, 08/18/2015 - 01:33
Campylobacter occur in fresh retail poultry products as a result of their colonization of the gastro-intestinal tract of chickens during growth. Feed additives could be used for suppression of Campylobacter levels in the chickens prior to slaughter. To address this opportunity, feed manufacturers are targeting natural antimicrobials from plant material as new forms of consumer-accepted feed...
Tue, 08/18/2015 - 01:33
Salmonella enterica is a zoonotic pathogen which can readily pass from animal to man through the consumption of contaminated food. The prevalence of Salmonella enterica associated with poultry and poultry meat products has been well-documented and this prevalence has both public health and economic implications. The estimated total cost for nontyphoidal Salmonella is in excess of 14 billion...
Tue, 08/18/2015 - 01:33
Campylobacter is one of the leading causes of foodborne illness worldwide and is associated with consumption of poultry products. One potential strategy to reduce Campylobacter colonization in poultry is the use of probiotics. In an attempt to reduce Campylobacter in poultry, we isolated 117 GRAS (generally recognized as safe) bacterial isolates from the ceca of healthy birds and evaluated their...
Tue, 08/18/2015 - 01:33
The human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. Whereas invasive pathogens cause epithelial barrier disruption, probiotic microorganisms modulate tight junction regulation and improve epithelial barrier function. In addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. The...
Mon, 08/17/2015 - 01:24
The antimicrobial and antioxidant effects of Citrus aurantium L. flowers (Bahar narang) extract (BE; 500, 1,000 and 2,000 ppm) were studied in traditional yoghurt stew during storage at 4C for 28 days. The contents of total phenolics and flavonoids in BE were 81 ± 1.8 and 46 ± 2.4 mg/g, respectively. In vitro antibacterial activity of BE showed that BE had a strong antimicrobial activity against...
Mon, 08/17/2015 - 01:24
The inhibitory activities of salvia and basil essential oils (EOs) against chemical and microbial spoilage in Iranian white cheese were investigated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration obtained for salvia against Listeria monocytogenes were 0.015 and 0.02%, and for basil were 0.05 and 0.06%, respectively. The activities of EOs against Aspergillus flavus...
Mon, 08/17/2015 - 01:24
In this study, 26 Streptococcus thermophilus phages were classified according to their structural protein patterns and DNA restriction fragment length polymorphism. Furthermore, sensitivities of natural and commercial S. thermophilus strains to S. thermophilus phages were investigated. All the natural strains were found to be resistant to the phages. The electrophoretic profiles of the structural...
Mon, 08/17/2015 - 01:24
The aim of this study was to examine the genetically mediated antimicrobial resistance in 94 coagulase-negative staphylococcal (CNS) milk isolates (buffalo, n = 88, and cow, n = 6), and to determine whether antimicrobial resistance profiles differed between bacterial species. Our analysis of 94 CNS isolates from milk confirmed the well-established multiresistant character of staphylococci in the...
Mon, 08/17/2015 - 01:24
Campylobacter have been found in lymphoid tissue of poultry, but the location in these tissues has not been determined. The objective of this study was to determine if Campylobacter are on the outside or on the inside of the spleen. For external (ES) spleen sampling 2 days post-inoculation, each spleen was rinsed with 3 mL of Bolton's enrichment broth (BEB). For internal (IS) spleen sampling,...
Mon, 08/17/2015 - 01:24
In this study, a detailed phytochemical analysis of the medicinal herb Inula viscosa leaves was performed. Furthermore, in vitro antioxidant and antifungal properties of its methanolic extract were evaluated and compared with the corresponding phenolic profile obtained by high-performance liquid chromatography and mass spectrometry. Data obtained underscore the high amount of total lipids (6.14...
Mon, 08/17/2015 - 01:24
With an Escherichia coli O157:H7 virulent bacteriophage, M8AEC16, biocontrol efficiency of phages on a highly risky, ready-to-eat, traditional delicacy food called “raw meatball” under different storage conditions was investigated. Phage, belonging to the Myoviridae family, was isolated from the wastewater of a local slaughterhouse and showed a broad lytic activity toward many E. coli O157:H7...
Mon, 08/17/2015 - 01:24
Spores of Bacillus megaterium were immobilized on a sensor disk by entrapment method followed by addition of pretreated milk and fluorogenic substrate, i.e., diacetate fluorescein with a response time of about 20 ± 5 min with ≥0.25 μg/L detection limit. Fluorescence was measured semiquantitatively using microbiological plate reader at specific excitation and emission spectra, i.e., 485 and 535 nm...
Mon, 08/17/2015 - 01:24
The aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of Mentha spicata plant against common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157:H7). Chemical composition of the essential oil was identified by gas...
Sun, 08/16/2015 - 01:20
10.1080/19440049.2015.1069407David Tennant
Sun, 08/16/2015 - 01:20
Publication date: February 2016 , Volume 60 Author(s): Shiquan Qian, Hedong Lu, Jing Sun, Chong Zhang, Haizhen Zhao, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu The inhibition effects of bacillomycin D on the growth of Aspergillus ochraceus and the production of ochratoxin A (OTA) in food samples were investigated. The mycelia growth and sporulation were completely inhibited by 30 μg/...
Sat, 08/15/2015 - 03:20
Botulinum neurotoxin (BoNT), derived from Clostridium botulinum, has been used therapeutically for focal dystonia, spasticity, and chronic migraine. Its spectrum as a potential treatment for neuropathic pain has grown. Recent opinions on the mechanism behind the antinociceptive effects of BoNT suggest that it inhibits the release of peripheral neurotransmitters and inflammatory mediators from...
Sat, 08/15/2015 - 03:20
Publication date: January 2016 Source:LWT - Food Science and Technology, Volume 65 Author(s): Qiumin Ma, Yue Zhang, Qixin Zhong Lauric arginate (LAE) and cinnamon oil (CO) combination results in synergistic antimicrobial effect on Gram-positive bacteria but antagonistic effect on Gram-negative bacteria. We recently observed that ethylenediaminetetraacetate (EDTA) enhanced the activity...
Sat, 08/15/2015 - 01:28
Publication date: February 2016 , Volume 60 Author(s): S. Belluco, L. Barco, A. Roccato, A. Ricci Background The slaughtering process consists of a highly coordinated system of different operations and is of critical importance for meat hygiene. The evaluation of the impact of different stages on bacterial counts is useful to monitor carcass contamination and to improve the hygiene of...
Sat, 08/15/2015 - 01:28
Publication date: February 2016 , Volume 60 Author(s): Qianqian Yu, Mengya Niu, Mengquan Yu, Yanhong Liu, Dapeng Wang, Xianming Shi Vibrio parahaemolyticus is a marine and estuarine bacterium that poses the greatest threat to human health worldwide. It has been the leading bacterial cause of seafood-borne illness. This study investigated the prevalence and drug resistance of V. ...
Sat, 08/15/2015 - 01:28
Publication date: February 2016 , Volume 60 Author(s): Céline Bacigalupi, Aurélie Maurey, Naïma Boutroy, Stéphane Peyron, Sylvie Avallone, Pascale Chalier The evolution of ascorbic acid, β-carotene and α-tocopherol contents was assessed during the storage of a fortified juice packed in PET or glass bottles. During the course of storage, oxygen transfer rates and partial oxygen...
Sat, 08/15/2015 - 01:28
Publication date: December 2015 , Volume 52 Author(s): Alexandre Ceugniez, Djamel Drider, Philippe Jacques, Françoise Coucheney This study is aimed at unrevealing the yeast diversity of handmade cheese, Tomme d'orchies, produced and marketed in the north of France. A total of 185 yeast colonies were isolated from the surface and core of this cheese. From these, 80 morphologically...
Sat, 08/15/2015 - 01:28
Publication date: December 2015 , Volume 52 Author(s): Pratheeba Yogendrarajah, Frank Devlieghere, Emmanuel Njumbe Ediage, Liesbeth Jacxsens, Bruno De Meulenaer, Sarah De Saeger A liquid chromatography triple quadrupole tandem mass spectrometry method was developed and validated to determine mycotoxins, produced by fungal isolates grown on malt extract agar (MEA). All twenty...
Sat, 08/15/2015 - 01:28
Shuzhen Liu, Ligang Jiang, Tao Zhong, Shuhui Kong, Rongbin Zheng, Fengyun Kong, Cong Zhang, Lei Zhang, Liguo An Acrylamide (ACR) is a chemical compound with severe neurotoxicity, genotoxicity, carcinogenicity and reproductive toxicity. Recent studies showed that ACR impairs the function of reproductive organs, e.g., epididymis and testes. In vitro maturation of mouse oocyte is a sensitive assay...

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