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Research Publications from Peer-Reviewed Journals

Fri, 07/01/2016 - 01:15
This article is currently available as a free download on Ingenta Connect et al.
Fri, 07/01/2016 - 00:52
10.1080/19440049.2016.1200750Liubo Pan
Fri, 07/01/2016 - 00:00
Botulinum neurotoxin A (BoNT-A), derived from Clostridium botulinum, has been used clinically for several diseases or syndrome including chronic migraine, spasticity, focal dystonia and other neuropathic pain. Chronic pelvic or bladder pain is the one of the core symptoms of bladder pain syndrome/interstitial cystitis (BPS/IC). However, in the field of urology, chronic bladder or pelvic pain is...
Thu, 06/30/2016 - 17:00
Hei Ming Lai, Alan King Lun Liu, Wai-Lung Ng, John DeFelice, Wing Sang Lee, Heng Li, Wen Li, Ho Man Ng, Raymond Chuen-Chung Chang, Bin Lin, Wutian Wu, Steve M. Gentleman Three-dimensional visualization of intact tissues is now being achieved turning tissues transparent. CLARITY is a unique tissue clearing technique, which features the use of detergents to remove lipids from fixed tissues to...
Thu, 06/30/2016 - 11:01
The ability to sense and adapt to temperature fluctuation is critical to the aquatic survival, transmission, and infectivity of Vibrio cholerae, the causative agent of the disease cholera. Little information is available on the physiological changes that occur when V. cholerae experiences temperature shifts. The genome-wide transcriptional profile of V. cholerae upon a shift in human body...
Thu, 06/30/2016 - 11:01
The heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans and animals. Our previous work demonstrated that oral administration of probiotics can significantly inhibit Cd absorption in the intestines of mice, but further evidence is needed to gain insights into the related protection mode. The goal of this study was to evaluate whether probiotics can...
Thu, 06/30/2016 - 11:01
Extracellular proteins play important roles in bacterial interactions with the environmental matrices. In this study, we examined the extracellular proteins from Escherichia coli O157:H7 and O104:H4 by tandem mass spectrometry. We identified 500 and 859 proteins from the growth media of E. coli O157:H7 and O104:H4, respectively, including 371 proteins common to both strains. Among proteins that...
Thu, 06/30/2016 - 11:01
Campylobacteriosis is one of the most important foodborne diseases worldwide and a significant health burden in New Zealand. Campylobacter jejuni is the predominant species worldwide, accounting for approximately 90% of human cases, followed by Campylobacter coli. Most studies in New Zealand have focused on C. jejuni; hence, the impact of C. coli strains on human health is not well understood....
Thu, 06/30/2016 - 11:01
Hybrid isolates of Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic E. coli (ETEC) encoding heat-stable enterotoxin (ST) are being reported with increasing frequency from a variety of sources. However, information regarding the plasmids that these strains harbor is scarce. In this study, we sequence and characterize a plasmid, p7v, from the STEC/ETEC hybrid strain 7v. Whole-...
Thu, 06/30/2016 - 11:01
Hepatitis E virus (HEV) is an increasingly recognized zoonotic pathogen. Transmission is suspected to occur from infected pigs or wild boars to humans through direct contact, environmental pathways, or contaminated food. However, the physical and chemical stability of HEV is largely unknown, because suitable cell culture methods for infectivity measurement are missing. Here, we developed a...
Thu, 06/30/2016 - 11:01
Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in...
Thu, 06/30/2016 - 11:01
Recurrent outbreaks of bacterial gastroenteritis linked to the consumption of fresh fruits and vegetables highlight the paucity of understanding of the ecology of Salmonella enterica under crop production and postharvest conditions. These gaps in knowledge are due, at least in part, to the lack of suitable surrogate organisms for studies for which biosafety level 2 is problematic. Therefore, we...
Thu, 06/30/2016 - 11:01
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was detected in 2003 and rapidly became the predominant MRSA clade in the Netherlands. Studies have shown that transmissions are difficult to identify, since this MRSA variant represents a genetically homogenous clade when current typing techniques are used. Here, next-generation sequencing was performed on 206 LA-MRSA...
Thu, 06/30/2016 - 08:30
Summary The role of amphibians as Salmonella reservoirs has not been as well studied as in reptiles, where the literature is abundant. Recent outbreaks of salmonellosis associated with exotic pet frogs have occurred in United States. Frog farming and wild frog harvesting have increased the international trade in these species. This necessitates a better understanding of the risk of salmonellosis...
Thu, 06/30/2016 - 03:20
Vicky Bronnec, Hana Turoňová, Agnès Bouju, Stéphane Cruveiller, Ramila Rodrigues, Katerina Demnerova, Odile Tresse, Nabila Haddad, Monique Zagorec
Thu, 06/30/2016 - 03:08
Abstract The development of rapid detection technologies in parallel with understanding production practices will markedly improve the microbiological safety of leafy vegetables. The objective of this study was on the application of phage-based magnetoelastic (ME) biosensors and optimization of selected surface blocking agents for a rapid detection for Salmonella Typhimurium on Romaine lettuce....
Thu, 06/30/2016 - 03:00
Summary Highly pathogenic avian influenza (HPAI) subtype H5N1 remains an enzootic disease of village chickens in Indonesia, posing ongoing risk at the animal–human interface. Previous modelling showed that the fast natural turnover of chicken populations might undermine herd immunity after vaccination, although actual details of how this effect applies to Indonesia's village chicken population...
Wed, 06/29/2016 - 17:00
Gopal Sapparapu, Rita Czakó, Gabriela Alvarado, Sreejesh Shanker, B. V. Venkataram Prasad, Robert L. Atmar, Mary K. Estes, James E. Crowe Jr. Noroviruses (NoV) are the most common cause of non-bacterial acute gastroenteritis and cause local outbreaks of illness, especially in confined situations. Despite being identified four decades ago, the correlates of protection against norovirus...
Wed, 06/29/2016 - 04:50
Abstract Bacterial flagella assembly is tightly regulated to ensure a timely and sequential production of the various flagellum constituents. In the pathogen Campylobacter jejuni the hierarchy in flagella biosynthesis is largely determined at the transcriptional level through the activity of the alternative sigma factors sigma54 and sigma28. Here we report that C. jejuni flagellin levels are also...
Wed, 06/29/2016 - 00:00
Esherichia coli O157 is pathogenic strain of Esherichia coli that is known to cause diarrhoea leading to fluid loss, electrolyte imbalance and other severe complications like haemolytic uraemic syndrome. This work was therefore aimed at isolating and serologically characterising Esherichia coli O157 from human stool with the set objectives of identifying the risk factors associated with diarrhoea...
Tue, 06/28/2016 - 19:35
The coating effects of Origanum vulgare oil and allicin combined with chitosan (CH) on the characteristics of Japanese sea bass (Lateolabrax japonicas) at 4 ± 0.5C storage were evaluated. Four different treatments were used: control without coating, chitosan coating (CH), chitosan-O. vulgare oil (0.5%) coating (CH + O) and chitosan-allicin (0.2%) coating (CH + A). Microbiological (total viable...
Tue, 06/28/2016 - 19:25
Several spices are used to process ‘Nduja, a traditional Calabrian sausage, well known for its typical flavor and piquancy. Despite wide use of spices for meat curing and controlling undesired microorganisms, no studies reported antimicrobial effectiveness of the spices in the ‘Nduja processing. The aim of this work was to evaluate the antimicrobial activities against 10 foodborne pathogens of...
Tue, 06/28/2016 - 19:25
The essential oil (EO) of Zataria multiflora Bioss. was successfully encapsulated into nanoliposomes in this study. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EO loaded nanoliposomes and free EO were studied at 25 and 35C against Escherichia coli O157:H7. Effect of sub-inhibitory concentrations (subMIC) of EO was also evaluated before and after...
Tue, 06/28/2016 - 18:34
Effect of storage conditions and packaging materials on quality parameters of curcuminoids infused coconut and raw banana slices were studied. Moisture sorption study was conducted for the developed products in selected packaging materials namely polypropylene (PP), polyethylene terephthalate (PET)/low density polyethylene terephthalate (LDPE) and metallized PET/LDPE, under ambient and...
Tue, 06/28/2016 - 17:00
Hilke Schroeder, Richard Cronn, Yulai Yanbaev, Tara Jennings, Malte Mader, Bernd Degen, Birgit Kersten To detect and avoid illegal logging of valuable tree species, identification methods for the origin of timber are necessary. We used next-generation sequencing to identify chloroplast genome regions that differentiate the origin of white oaks from the three continents; Asia, Europe, and North...
Tue, 06/28/2016 - 16:45
Although food-borne outbreaks of listeriosis are uncommon, they remain a major public health problem. We assessed the prevalence of Listeria monocytogenes in prepared foods and the influence of different factors (including type of food or presence of accompanying bacteria). Results showed that accompanying bacteria cause interference in the sensitivity of the detection method, being half Fraser...
Tue, 06/28/2016 - 16:15
Oxidation is a growing concern for quality deterioration of meat and meat products as it adversely affects sensory and nutritional quality. Various synthetic oxidants have been used as counter agents to prevent oxidation in meat and meat products but the application of these compounds has been questioned due to their safety concerns. Nowadays consumers have become health conscious and they...
Tue, 06/28/2016 - 13:02
RNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to...
Tue, 06/28/2016 - 04:31
Abstract Bacterial ribosome requires elongation factor P to translate fragments harboring consecutive proline codons. Given the abundance of ORFs with potential EF-P regulated sites, EF-P was assumed to be constitutively expressed. Here, we report that the intracellular pathogen Salmonella enterica serovar Typhimurium decreases efp mRNA levels during course of infection. We determined that the...
Tue, 06/28/2016 - 03:57
Publication date: January 2017 , Volume 71 Author(s): Sasikala Remya, Chitradurga Obayya Mohan, Gudipati Venkateshwarlu, Gopal Krishna Sivaraman, Chandragiri Nagarajarao Ravishankar The present study was carried out to understand the combined effect of O2 scavenger (OS) and antimicrobial film (AM) in extending the shelf life of fresh cobia (Rachycentron canadum) fish steaks stored...
Tue, 06/28/2016 - 03:57
Publication date: January 2017 , Volume 71 Author(s): Yi-Gang Xu, Liu-Mei Sun, Yu-Sai Wang, Pei-Pei Chen, Zhong-Mei Liu, Yi-Jing Li, Li-Jie Tang In this study, a rapid and reliable multiplex PCR assay for simultaneous detection of Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio alginolyticus in seafood was developed using the dual priming oligonucleotide (...
Tue, 06/28/2016 - 03:57
Publication date: January 2017 , Volume 71 Author(s): Hamed K. Abbas, W. Thomas Shier, Javier Plasencia, Mark A. Weaver, Nacer Bellaloui, Jeremy K. Kotowicz, Alemah M. Butler, Cesare Accinelli, M. Eugenia de la Torre-Hernandez, Robert M. Zablotowicz Corn infected with Ustilago maydis, causal agent of common smut disease, produces galls that are used as food in certain...
Tue, 06/28/2016 - 03:20
Darío Ortiz de Orué Lucana, Neal Hickey, Michael Hensel, Johann P. Klare, Silvano Geremia, Tatiana Tiufiakova, Andrew E. Torda
Tue, 06/28/2016 - 00:00
Paralytic shellfish toxins (PST) traditionally have been analyzed by liquid chromatography with either pre- or post-column derivatization and always with a silica-based stationary phase. This technique resulted in different methods that need more than one run to analyze the toxins. Furthermore, tetrodotoxin (TTX) was recently found in bivalves of northward locations in Europe due to climate...
Mon, 06/27/2016 - 17:00
Whitney B. Hapke, Jennifer L. Morace, Elena B. Nilsen, David A. Alvarez, Kevin Masterson Pesticide presence in streams is a potential threat to Endangered Species Act listed salmonids in the Hood River basin, Oregon, a primarily forested and agricultural basin. Two types of passive samplers, polar organic chemical integrative samplers (POCIS) and semipermeable membrane devices (SPMDs), were...

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