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Research Publications from Peer-Reviewed Journals

Tue, 03/08/2016 - 00:00
Cholesterol is an essential structural component of cellular membranes in eukaryotes. Cholesterol in the exofacial leaflet of the plasma membrane is thought to form membrane nanodomains with sphingolipids and specific proteins. Additionally, cholesterol is found in the intracellular membranes of endosomes and has crucial functions in membrane trafficking. Furthermore, cellular cholesterol...
Mon, 03/07/2016 - 14:30
Publication date: Available online 7 March 2016 Author(s): Hyunjoon Park, Heuynkil Shin, Kyuyeon Lee, Wilhelm Holzapfel Bacteria use the cell density-dependent quorum signalling system to regulate particular gene expressions. In food microbiology, signalling is well known for its relation to (foodborne) pathogenicity, food spoilage, and biofilm formation. Quorum quenching and...
Mon, 03/07/2016 - 11:01
We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. When the F. novicida ligA hybrid strains were plated above their restrictive...
Mon, 03/07/2016 - 11:01
The marine foodborne enteropathogen Vibrio parahaemolyticus has four putative catalase genes. The functions of two katE-homologous genes, katE1 (VPA1418) and katE2 (VPA0305), in the growth of this bacterium were examined using gene deletion mutants with or without complementary genes. The growth of the mutant strains in static or shaken cultures in a rich medium at 37°C or at low temperatures...
Mon, 03/07/2016 - 11:01
As a nonthermal sterilization technique, ultrasound has attracted great interest in the field of food preservation. In this study, flow cytometry and transmission electron microscopy were employed to investigate ultrasound-induced damage to Escherichia coli and Staphylococcus aureus. For flow cytometry studies, single staining with propidium iodide (PI) or carboxyfluorescein diacetate (cFDA)...
Mon, 03/07/2016 - 11:01
The Nycteribiidae are obligate blood-sucking Diptera (Hippoboscoidea) flies that parasitize bats. Depending on species, these wingless flies exhibit either high specialism or generalism toward their hosts, which may in turn have important consequences in terms of their associated microbial community structure. Bats have been hypothesized to be reservoirs of numerous infectious agents, some of...
Mon, 03/07/2016 - 11:01
Cover crops provide several ecosystem services, but their impact on enteric bacterial survival remains unexplored. The influence of cover cropping on foodborne pathogen indicator bacteria was assessed in five cover crop/green manure systems: cereal rye, hairy vetch, crimson clover, hairy vetch-rye and crimson clover-rye mixtures, and bare ground. Cover crop plots were inoculated with Escherichia...
Mon, 03/07/2016 - 11:01
The rapid rise in DNA sequencing has led to an expansion in the number of glycoside hydrolase (GH) families. The GH43 family currently contains α-l-arabinofuranosidase, β-d-xylosidase, α-l-arabinanase, and β-d-galactosidase enzymes for the debranching and degradation of hemicellulose and pectin polymers. Many studies have revealed finer details about members of GH43 that...
Mon, 03/07/2016 - 11:01
We have produced data and developed analysis to build representations for the concentration of spores of nonproteolytic Clostridium botulinum in materials that are used during the manufacture of minimally processed chilled foods in the United Kingdom. Food materials are categorized into homogenous groups which include meat, fish, shellfish, cereals, fresh plant material, dairy liquid, dairy...
Mon, 03/07/2016 - 10:15
Abstract AimsThe objective of this work was to study the growth potential of E. coli O157:H7 and Salmonella spp. in leafy vegetable extracts at different temperature conditions. Methods and ResultsCocktails of five strains of E. coli O157: H7 and of S. enterica were used. Inoculated aqueous vegetable extracts were incubated at 8, 10, 16 and 20°C during 21 days. Microbial growth was monitored...
Sun, 03/06/2016 - 06:48
Abstract In this study, we comprehensively investigated the effect of dietary protein sources on the gut microbiome of weaned piglets with diets comprising different protein source using High-throughput 16SrRNA gene-based Illumina Miseq. A total of 48 healthy weaned piglets were allocated randomly to four treatments with 12 piglets in each group. The weaned piglets were fed with diets containing...
Sat, 03/05/2016 - 20:20
Publication date: September 2016 , Volume 67 Author(s): Honglei Zhan, Jianfeng Xi, Kun Zhao, Rima Bao, Lizhi Xiao Effective technique and procedure based on the terahertz (THz) spectroscopy and mathematical methods, named THz Mathematics (T-Math), have been developed for the precise and rapid identification of edible oil from a series of typical swill-cooked dirty oils in daily life...
Sat, 03/05/2016 - 17:42
Publication date: July 2016 , Volume 117 Author(s): K.S. Steele, M.J. Weber, E.A.E. Boyle, M.C. Hunt, A.S. Lobaton-Sulabo, C. Cundith, Y.H. Hiebert, K.A. Abrolat, J.M. Attey, S.D. Clark, D.E. Johnson, T.L. Roenbaugh Enhanced pork loin chops, beef longissimus lumborum steaks, semimembranosus steaks (superficial and deep portions), ground beef, and ground turkey were...
Sat, 03/05/2016 - 10:53
Publication date: July 2016 Source:LWT - Food Science and Technology, Volume 70 Author(s): Lin Bi, Lei Yang, Arun K. Bhunia, Yuan Yao ɛ-Poly-l-lysine (EPL) is a food-grade cationic antimicrobial compound with a wide antimicrobial spectrum against bacteria, yeasts, and molds. However, EPL can be subject to rapid depletion after initial application and lose activity quickly. To address...
Sat, 03/05/2016 - 07:09
Infection with Yersinia enterocolitica causes acute diarrhea in early childhood. A mouse infection model presents new findings on pathological mechanisms in the colon. Symptoms involve diarrhea with watery feces and weight loss that have their functional correlates in decreased transepithelial electrical resistance and increased fluorescein permeability. Y. enterocolitica was present within the...
Fri, 03/04/2016 - 23:01
Mitigation of Salmonella associated with poultry carcasses is primarily accomplished by rendering or carcass composting. While rendering temperatures and pressures are well established for pathogen inactivation in poultry carcasses, parameters controlling composting processes are less defined in part because multiple conditions and procedures are utilized. Consequently, limited knowledge exists...
Fri, 03/04/2016 - 23:01
Two experiments evaluated prebiotics added to feed on the recovery of Salmonella in broilers during grow-out and processing. In Experiment 1, "seeder" chicks were inoculated with Salmonella Typhimurium and placed with penmates. Treatments were: basal control diet, added 0.3% bamboo charcoal, 0.6% bamboo charcoal, or 0.12% Aromabiotic (medium chain fatty acids). The ceca from seeders and...
Fri, 03/04/2016 - 23:01
While horizontal transmission is a route clearly linked to the spread of Campylobacter at the farm level, few studies support the transmission of Campylobacter spp. from breeder flocks to their offspring. Thus, the present study was carried out to investigate the possibility of vertical transmission. Breeders were monitored from the time of housing day-old chicks, then throughout the laying...
Fri, 03/04/2016 - 23:01
The present study evaluated the efficacy of recently approved Salmonella lytic bacteriophage preparation (SalmoFresh™) in reducing Salmonella on chicken breast fillets, as a surface and dip application. The effectiveness of phage in combination with modified atmosphere packaging (MAP) and the ability of phage preparation in reducing Salmonella on chicken breast fillets at room temperature...
Fri, 03/04/2016 - 20:14
Publication date: September 2016 , Volume 67 Author(s): J. Brändle, K.J. Domig, W. Kneifel Via butyric acid fermentation, clostridia – mainly Clostridium tyrobutyricum – are able to transform lactic acid into butyric acid, acetic acid and gas (H2 and CO2). The presence of clostridial endospores in milk may lead to severe quality defects in semi-hard and hard cheeses. As a consequence of...
Fri, 03/04/2016 - 20:14
Publication date: September 2016 , Volume 67 Author(s): Francisco Lopez-Galvez, Maria Isabel Gil, Francisco Pedrero-Salcedo, Juan Jose Alarcón, Ana Allende Systematic monitoring of indicator microorganisms, such as Escherichia coli, can help to identify potential risk factors for faecal contamination in the agricultural environment. In this study, levels of E. coli in irrigation...
Fri, 03/04/2016 - 17:00
Jie Ren, Yu Sang, Yongcong Tan, Jing Tao, Jinjing Ni, Shuting Liu, Xia Fan, Wei Zhao, Jie Lu, Wenjuan Wu, Yu-Feng Yao The two-component system PhoP-PhoQ is highly conserved in bacteria and regulates virulence in response to various signals for bacteria within the mammalian host. Here, we demonstrate that PhoP could be acetylated Pat and deacetylated deacetylase CobB enzymatically in vitro and in...
Fri, 03/04/2016 - 16:44
Summary Listeria monocytogenes FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the Bacillus subtilis Fur- and PerR-regulated Fe(II) efflux P1B4-type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an frvA mutant is sensitive to iron intoxication, but not to other metals. Expression of frvA is induced by high...
Fri, 03/04/2016 - 10:03
Publication date: Available online 4 March 2016 Author(s): Megan E. Meek, Frances M. Van Dolah Abstract Phytoplankton rarely occur as unialgal populations. Therefore, to study species-specific protein expression, indicative of physiological status in natural populations, methods are needed that will both assay for a protein of interest and identify the species expressing it. Here we...
Fri, 03/04/2016 - 05:50
Abstract This study developed growth/no growth models to describe the effect of NaNO2 and NaCl on Enterococcus growth. Enterococcus spp. were incubated in deMan, Rogosa and Sharpe broth formulated with NaNO2 (0–210 ppm) and NaCl (0–1.75%) at 4–15C up to 1,080 h under aerobic and anaerobic conditions. Growth responses (growth: 1 or no growth: 0) for all the combinations were collected. The growth...
Fri, 03/04/2016 - 02:14
Publication date: Available online 3 March 2016 Food and Chemical Toxicology Author(s): Bas J. Blaauboer, Alan R. Boobis, Bobbie Bradford, Andrew Cockburn, Anne Constable, Mardas Daneshian, Gareth Edwards, Jossie A. Garthoff, Brett Jeffery, Cyrille Krul, Jeroen Schuermans Toxicology and safety assessment are changing and require new strategies for evaluating risk that...

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