You are here

Research Publications from Peer-Reviewed Journals

Mon, 02/08/2016 - 12:27
The general stress response (GSR) system of the intracellular pathogen Brucella abortus controls transcription of approximately 100 genes in response to a range of stress cues. The core genetic regulatory components of the GSR are required for B. abortus survival under non-optimal growth conditions in vitro, and for maintenance of chronic infection in an in vivo mouse model. The functions of the...
Mon, 02/08/2016 - 09:00
Abstract AimsThe aim of the study was to determine the prevalence of Mycobacterium bovis (the causative agent of bovine tuberculosis, bTB) in environmental matrices within a French region (Côte d'Or) affected by this zoonotic disease. Methods and ResultsWe report here the development and the use of molecular detection assays based on qPCR (double fluorescent dye labelled probe) to monitor the...
Mon, 02/08/2016 - 05:20
Abstract Listeria monocytogenes is an important foodborne pathogen that can cause infection in children, pregnant women, the immunocompromised and the elderly. Antibiotic resistance in this species would represent a significant public health problem since the organism has a high fatality/case ratio and resistance may contribute to failure of therapeutic treatment. This study was designed to...
Mon, 02/08/2016 - 04:34
Publication date: Available online 6 February 2016 Author(s): B. Kramer, J. Thielmann Rapid microbiological methods to assess cell physiological properties of microorganisms are gaining interest in the elucidation of the effect of antimicrobial agents or physical inactivation. Fluorescent probes combined with flow cytometry or microplate assays provide information about cellular targets...
Mon, 02/08/2016 - 02:21
Summary The control of Salmonella enterica in pig production is necessary for both public and animal health. The persistent and frequently asymptomatic nature of porcine Salmonella infection and the organism's abilities to colonize other animal species and to survive in the environment mean that effective control generally requires multiple measures. Vaccination is one such measure, and the...
Sun, 02/07/2016 - 10:40
Publication date: Available online 6 February 2016 Author(s): Monika Dzieciol, Elisa Schornsteiner, Meryem Muhterem-Uyar, Beatrix Stessl, Martin Wagner, Stephan Schmitz-Esser Sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. Floor drain water serves along with product-associated...
Sat, 02/06/2016 - 06:14
10.1080/19440049.2015.1136844Yolanda Echegoyen
Sat, 02/06/2016 - 05:09
Publication date: August 2016 , Volume 66 Author(s): Xu-Cong Lv, Yan Li, Wan-Wei Qiu, Xiao-Qi Wu, Bing-Xin Xu, Yi-Ting Liang, Bin Liu, Shao-Jun Chen, Ping-Fan Rao, Li Ni The main disadvantage of DNA-based quantification methods is their inability to distinguish between viable and non-viable cells, as dead cells may also retain a significant amount of DNA. To overcome this...
Sat, 02/06/2016 - 05:09
Publication date: August 2016 , Volume 66 Author(s): Haiyan Zhao, Shuangling Zhang The objective of this study was to select suitable elements which reflect the link with geographical origin while are less affected by season to build robust discriminant models for the protection of Jiaozhou Bay clam by the multi-element fingerprinting technique. Manila clam (Ruditapes philippinarum),...
Sat, 02/06/2016 - 00:00
Deoxynivalenol (DON), which is a toxic secondary metabolite generated by Fusarium species, is synthesized through two separate acetylation pathways. Both acetylation derivatives, 3-acetyl-DON (3ADON) and 15-acetyl-DON (15ADON), also contaminate grain and corn widely. These derivatives are deacetylated via a variety of processes after ingestion, so it has been suggested that they have the same...
Fri, 02/05/2016 - 17:00
Alicja K. Warda, Marcel H. Tempelaars, Jos Boekhorst, Tjakko Abee, Masja N. Nierop Groot Spores are widely present in the environment and are common contaminants in the food chain, creating a challenge for food industry. Nowadays, heat treatments conventionally applied in food processing may become milder to comply with consumer desire for products with higher sensory and nutritional values....
Fri, 02/05/2016 - 17:00
Raies A. Mir, Thomas A. Weppelmann, Mauricio Elzo, Soohyoun Ahn, J. Danny Driver, KwangCheol Casey Jeong Each year Shiga toxin-producing Escherichia coli (STEC) are responsible for 2.8 million acute illnesses around the world and > 250,000 cases in the US. Lowering the prevalence of this pathogen in animal reservoirs has the potential to reduce STEC outbreaks in humans controlling its...
Fri, 02/05/2016 - 17:00
Edgar Balcázar-López, Luz Helena Méndez-Lorenzo, Ramón Alberto Batista-García, Ulises Esquivel-Naranjo, Marcela Ayala, Vaidyanathan Vinoth Kumar, Olivier Savary, Hubert Cabana, Alfredo Herrera-Estrella, Jorge Luis Folch-Mallol Fungal laccases are enzymes that have been studied because of their ability to decolorize and detoxify effluents; they are also used in paper bleaching, synthesis of...
Fri, 02/05/2016 - 11:01
During their colonization of plants, human enteric pathogens, such as Salmonella enterica, are known to benefit from interactions with phytopathogens. At least in part, benefits derived by Salmonella from the association with a soft rot caused by Pectobacterium carotovorum were shown to be dependent on Salmonella KdgR, a regulator of genes involved in the uptake and utilization of carbon sources...
Fri, 02/05/2016 - 11:01
Increasing evidence indicates that despite exposure to harsh environmental stresses, Salmonella enterica successfully persists on plants, utilizing fresh produce as a vector to animal hosts. Among the important S. enterica plant colonization factors are those involved in biofilm formation. S. enterica biofilm formation is controlled by the signaling molecule cyclic di-GMP and represents a sessile...
Fri, 02/05/2016 - 11:01
Wild waterfowl are important reservoir hosts for influenza A virus (IAV) and a potential source of spillover infections in other hosts, including poultry and swine. The emergence of highly pathogenic avian influenza (HPAI) viruses, such as H5N1 and H5N8, and subsequent spread along migratory flyways prompted the initiation of several programs in Europe, North America, and Africa to monitor...
Fri, 02/05/2016 - 11:01
Listeria monocytogenes is a saprophytic bacterium that thrives in diverse environments and causes listeriosis via ingestion of contaminated food. RsbX, a putative sigma B (B) regulator, is thought to maintain the ready state in the absence of stress and reset the bacterium to the initial state in the poststress stage in Bacillus subtilis. We wondered whether RsbX is functional in L. monocytogenes...
Fri, 02/05/2016 - 11:01
Cochlodinium polykrikoides is a cosmopolitan dinoflagellate that is notorious for causing fish-killing harmful algal blooms (HABs) across North America and Asia. While recent laboratory and ecosystem studies have definitively demonstrated that Cochlodinium forms resting cysts that may play a key role in the dynamics of its HABs, uncertainties regarding cyst morphology and detection have...
Fri, 02/05/2016 - 11:01
Vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. The primary virulence factors associated with V. parahaemolyticus illness are thermostable direct hemolysin (TDH) and Tdh-related hemolysin (TRH). However, clinical strains lacking tdh and trh have recently been isolated, and these clinical isolates are poorly...
Fri, 02/05/2016 - 11:01
Shiga toxin-producing Escherichia coli (STEC) serotype O145 is one of the major non-O157 serotypes associated with severe human disease. Here we examined the genetic diversity, population structure, virulence potential, and antimicrobial resistance profiles of environmental O145 strains recovered from a major produce production region in California. Multilocus sequence typing analyses revealed...
Fri, 02/05/2016 - 11:01
The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much greater than those of other organic acids (acetic, citric, lactic, and malic acids...
Fri, 02/05/2016 - 11:01
N-Glycosylation can modulate enzyme structure and function. In this study, we identified two pepsin-resistant histidine acid phosphatase (HAP) phytases from Yersinia kristensenii (YkAPPA) and Yersinia rohdei (YrAPPA), each having an N-glycosylation motif, and one pepsin-sensitive HAP phytase from Yersinia enterocolitica (YeAPPA) that lacked an N-glycosylation site. Site-directed mutagenesis was...
Fri, 02/05/2016 - 04:09
Publication date: Available online 4 February 2016 Author(s): Bongkot Ngamsom, Maria J. Lopez-Martinez, Jean-Claude Raymond, Patrick Broyer, Pradip Patel, Nicole Pamme Pathogen analysis in food samples routinely involves lengthy growth-based pre-enrichment and selective enrichment of food matrices to increase the ratio of pathogen to background flora. Similarly, for blood culture...
Fri, 02/05/2016 - 04:09
Publication date: Available online 4 February 2016 Author(s): Judit K. Kovács, Györgyi Horváth, Monika Kerényi, Béla Kocsis, Levente Emődy, György Schneider Direct bioautography is a useful method to identify antimicrobial compounds with potential therapeutic importance. Because of technical limitations till now, it has been applied only for aerobic bacteria. In this work we...
Fri, 02/05/2016 - 01:14
Abstract This study investigated the effect of functional properties of barberry aqueous extract in different concentration of 4 and 5% (w/w) on the growth and survival of probiotic cultures (L. acidophilus, B. bifidum and an equal mixture of the two strains) in the set and stirred yogurt samples. The viable counts of L. acidophilus and B. bifidum in the set and stirred probiotic yogurts...
Fri, 02/05/2016 - 00:00
Ochratoxins, and particularly ochratoxin A (OTA), are toxic fungal-derived contaminants of food and other agricultural products. Growing evidence supports the degradation of OTA by chemical, enzymatic and/or microbial means as a potential approach to remove this mycotoxin from food products. In particular, hydrolysis of OTA to ochratoxin α (OTα) and phenylalanine is the presumptive product of...
Thu, 02/04/2016 - 17:46
Abstract Two-component systems (TCSs) play important roles in the adaptation of bacteria to stress. Despite their increasingly well understood mechanistic features, it remains poorly understood how TCSs transduce signals across membranes. Here, we use the E. coli Cu/Ag-responsive CusSR TCS as a model to investigate the roles of CusS transmembrane (TM) residues. Proline scanning of TM1 domain led...
Thu, 02/04/2016 - 17:00
Jin-Ying Cui, Han Miao, Li-Hong Ding, Todd C. Wehner, Pan-Na Liu, Ye Wang, Sheng-Ping Zhang, Xing-Fang Gu Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore their use growers can reduce pesticide residues. Previous studies have reported two glabrous mutant plants containing the genes,...
Thu, 02/04/2016 - 11:43
Publication date: August 2016 , Volume 66 Author(s): Shin-Young Lee, Mi-Ju Kim, Yeun Hong, Hae-Yeong Kim On-site detection with minimal equipment and no risk of contamination of samples is crucial for the rapid and sensitive identification of pork in processed meat products. To resolve this issue, a reliable loop-mediated isothermal amplification (LAMP) method was developed to detect...
Thu, 02/04/2016 - 11:43
Publication date: August 2016 , Volume 66 Author(s): M. Haseeb Ahmad, Marius Nache, Stephanie Waffenschmidt, Bernd Hitzmann Fluorescence spectroscopy provides an ideal tool to explore chemical changes during dough mixing process. This paper aims to make use of this tool to investigate the influence of hydration of flour onto the spectral signals, classification of farinographic curve...
Thu, 02/04/2016 - 10:36
Publication date: Available online 3 February 2016 Author(s): Runze Huang, Mu Ye, Xinhui Li, Lin Ji, Mukund Karwe, Haiqiang Chen Human norovirus (HuNoV) has been an increasing concern of foodborne illness related to fresh and frozen berries. In this study, high hydrostatic pressure (HHP) inactivation of HuNoV on fresh strawberries, blueberries, and raspberries and in their purees...
Thu, 02/04/2016 - 10:36
Publication date: 2 April 2016 , Volume 222 Author(s): Yinqiang Cai, Jing Tao, Yang Jiao, Xiao Fei, Le Zhou, Yan Wang, Huijuan Zheng, Zhiming Pan, Xinan Jiao An epidemiological investigation of Salmonella spp. in pig and pork samples from one slaughterhouse and its downstream retail markets in Yangzhou, Jiangsu Province, China, was conducted from October 2013 to March 2014....
Thu, 02/04/2016 - 07:58
Publication date: Available online 3 February 2016 Author(s): Conrado Carrascosa, Rafael Millán, Pedro Saavedra, José Raduán Jaber, António Raposo, Esther Sanjuán The purpose of this paper was to evaluate, by statistical analyses, risk factors on cheese farms that can influence the microbial contamination of their products. Various assessment tools, such as cheese production...
Thu, 02/04/2016 - 07:58
Publication date: Available online 3 February 2016 Author(s): R. Wolf, K. Orsel, J. De Buck, U. Kanevets, H.W. Barkema Mycobacterium avium ssp. paratuberculosis (MAP) causes Johne's disease, a production-limiting disease in cattle. Detection of infected herds is often done using environmental samples (ES) of manure, which are collected in cattle pens and manure storage areas....
Thu, 02/04/2016 - 07:58
Publication date: Available online 3 February 2016 Author(s): S. Gutiérrez, H. Martínez-Blanco, L.B. Rodríguez-Aparicio, M.A. Ferrero In this study, the effect that 5 fermented broths of lactic acid bacteria (LAB) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. The fermented broth from Lactococcus lactis C660 had a...
Thu, 02/04/2016 - 04:04
Publication date: Available online 3 February 2016 Author(s): Mingxing Tian, Jing Qu, Yanqing Bao, Jianpeng Gao, Jiameng Liu, Shaohui Wang, Yingjie Sun, Chan Ding, Shengqing Yu Brucellosis, the most common widespread zoonotic disease, is caused by Brucella spp., which are facultative, intracellular, Gram-negative bacteria. With the development of molecular biology...
Wed, 02/03/2016 - 11:48
The main objective of this study was to determine the effects of various levels of dietary Nigella sativa seeds on immune-responsiveness and pathogenesis of H9N2 avian influenza virus (AIV) in turkeys. The experiment was performed on 80 unvaccinated turkey poults, divided into four groups of 20 birds each. Group A was kept as a non-infected, non-treated negative control; group B was inoculated...
Wed, 02/03/2016 - 11:48
Acid injury could prevent detection of Salmonella in feed and feed-type samples. A previous study showed that after incubation in commonly used pre-enrichment media, mixed feeds and feed ingredients reached a pH (4.0 to 5.0), capable of injuring or killing Salmonella. Approximately 105 colony forming units (CFU) of S. Enteritidis (SE), S. Heidelberg (SH), S. Kentucky (SK), or S. Typhimurium (ST)...
Wed, 02/03/2016 - 11:48
The enumeration and evaluation of bacteria and mold are important elements in the evaluation of hatchery sanitation programs. However, detection of bacteria and mold in fluff samples is time consuming and cumbersome. The PetrifilmTM aerobic count plate (ACP) and the Petrifilm yeast/mold count plate are ready-to-use alternatives to the traditional enumeration media used for bacteria and mold...
Wed, 02/03/2016 - 11:48
A study was conducted examining the effects of prebiotics and probiotics separately and in combination on growth parameters, fecal and cecal microbiota. Six dietary treatments consisted of: 1) control, 2) control + 0.2 lb/ton calculated to contain 1 x 1010 Pediococcus acidilactici/kg, 3) control + 0.2 lb/ton calculated to contain 2 x 1010 live Saccharomyces cerevisiae boulardii/kg, 4) control + 2...
Wed, 02/03/2016 - 11:42
Publication date: August 2016 , Volume 66 Author(s): Chia-Hao Chang, Han-Yang Lin, Qiu Ren, Yeong-Shin Lin, Kwang-Tsao Shao Mislabeling of fish products not only impacts consumer finances, but can also be deleterious to public health. Fish products may be mislabeled for reasons including ambiguity of common fish names, challenging morphological identification, or willful intention...
Wed, 02/03/2016 - 11:42
Publication date: August 2016 , Volume 66 Author(s): Matthias Schmutzler, Christian W. Huck Antioxidant capacity and total soluble solids content (SSC) of seven cultivars of apples (Braeburn, Evelina, Gala, Golden Delicious, Granny Smith, Jonagold, Pink Lady) were successfully determined applying a FT-NIR handheld spectrometer. On-site prediction of SSC of 92 apples was possible applying...
Tue, 02/02/2016 - 13:35
Abstract AimsThis study observed the behavior of probiotics and selected bacterial pathogens co-inoculated into peanut butter during gastrointestinal simulation. Methods and ResultsPeanut butter homogenates co-inoculated with Salmonella/Listeria strains (5 log CFU ml−1) and lyophilized or cultured probiotics (9 log CFU ml−1) were exposed to simulated gastrointestinal conditions for 24 h at 37°C....
Tue, 02/02/2016 - 11:41
Publication date: August 2016 , Volume 66 Author(s): Xin-ting Yang, Jian-ping Qian, Jie Li, Zeng-tao Ji, Bei-lei Fan, Bin Xing, Wen-yong Li Counterfeiting products and abusing labels lead to less credibility for traceability system in China recently. Authentication and supervision agencies driven by government departments play an important role for ensuring the quality safety in...

Pages