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Research Publications from Peer-Reviewed Journals

Wed, 02/18/2015 - 03:20
Direct analysis in real time (DART) ionisation coupled to mass spectrometry (MS) was used for the rapid quantitative analysis of T-2 toxin (T-2) and the related HT-2 toxin (HT-2), extracted from maize. Sample preparation procedures and instrument parameters were optimised to obtain sensitive and accurate determination of the toxins. The lowest calibration levels were 50 μg/kg for T-2 and 300 μg/...
Wed, 02/18/2015 - 03:20
Aflatoxins are a class of mycotoxins that impair poultry health and performance. Some clays have the ability to adsorb aflatoxins. In our study, mineralogical composition of the tested clays was confirmed by powder X-ray diffraction and simultaneous thermal analysis and their properties like pH, electrical conductivity, exchangeable cations, cation exchange capacity and clay content were...
Wed, 02/18/2015 - 03:20
The aim of present study was to reveal the toxicokinetic properties and absolute oral bioavailability of deoxynivalenol (DON) in turkey poults. Six turkey poults were administered this Fusarium mycotoxin per os and intravenously in a two-way cross-over design. Based on non-compartmental analysis, DON was absorbed rapidly (Tmax= 0.57 h) but incomplete, as the oral bioavailability was only 20.9%....
Wed, 02/18/2015 - 01:23
Publication date: August 2015 , Volume 54 Author(s): Conrado Carrascosa , Rafael Millán , Jose Raduán Jaber , Pablo Lupiola , Cristóbal del Rosario-Quintana , Cristina Mauricio , Esther Sanjuán This study describes the process of identifying the causative agent of a blue pigment on the surface of fresh raw cheese. It was studied using traditional microbiological methods and genotypic...
Wed, 02/18/2015 - 01:23
Publication date: August 2015 , Volume 54 Author(s): Joris Van Loco , Stefanie Vandevijvere , Oya Cimenci , Christine Vinkx , Séverine Goscinny The safety of the use of food additives is evaluated through the intake assessment of the additives for the consumer. A stepwise approach (using EFSA's Food Additives intake model (FAIM), followed by Tier 2 and refined Tier 2) was used to assess...
Wed, 02/18/2015 - 01:23
Publication date: August 2015 , Volume 54 Author(s): Filomena Silva , Cristina Nerín , Fernanda C. Domingues Due to the highly epidemic character of Campylobacter infections and to the increased antibiotic resistance of these microorganisms, new approaches to control them are highly demanded. In this work, we investigated the anti- Campylobacter activity and mode of action of stilbenes...
Wed, 02/18/2015 - 01:23
Giardia intestinalis is a pathogen associated with foodborne outbreaks and Escherichia coli is commonly used as a marker of faecal contamination. Implementation of routine identification methods of G. intestinalis is difficult for the analysis of vegetables and the microbiological detection of E. coli requires several days. This study proposes a PCR-based assay for the detection of E. coli and G...
Tue, 02/17/2015 - 01:16
Five enterococcal strains (Enterococcus faecalis LS15, PF03, BF10 and SL13 and Enterococcus faecium MM08) were isolated and identified from 130 probiotic products marketed in China. The determined biological characteristics were acid tolerance, bile tolerance, pepsin tolerance, trypsin tolerance, organic acid production and antimicrobial activity. Safety examination of potential virulence factors...
Tue, 02/17/2015 - 01:16
Loop-mediated isothermal amplification (LAMP) based on lmo0460 sequence in genome of Listeria monocytogenes (L. monocytogenes) was designed for detection of L. monocytogenes, which is an important foodborne kind of pathogenic bacteria causing human and animal disease. The primers set for lmo0460 sequence encoding membrane-associated lipoprotein consist of four specific primers targeting six...
Tue, 02/17/2015 - 01:16
The aim of this study is to investigate the antibacterial characteristics and mechanisms of nutmeg oil. In this paper, 54 chemical compositions of nutmeg oil were analyzed by gas chromatography–mass spectrometry (GC–MS). The major volatile components of nutmeg oil were sabinene (39.12%) and alpha-pinene (11.96%). In addition, nutmeg oil showed significant antibacterial activity, minimum...
Tue, 02/17/2015 - 01:16
Hongxia Wang, Julio C. Ayala, Jorge A. Benitez, Anisia J. Silva The histone-like nucleoid structuring protein (H-NS) functions as a transcriptional silencer by binding to AT-rich sequences at bacterial promoters. However, H-NS repression can be counteracted by other transcription factors in response to environmental changes. The identification of potential toxic factors, the expression of which...
Tue, 02/17/2015 - 01:16
Publication date: 15 July 2015 , Volume 179 Author(s): H. Okyere , R.B. Voegborlo , S.E. Agorku Total mercury (Hg), cadmium (Cd) and lead (Pb) concentrations were determined in canned fish on the Ghanaian market. Total mercury was determined using an automatic mercury analyzer while cadmium and lead levels were determined by flame atomic absorption spectrophotometry. The metal contents in...
Tue, 02/17/2015 - 01:16
Publication date: 15 July 2015 , Volume 179 Author(s): Qiao-Juan Yan , Lin-Hua Huang , Qian Sun , Zheng-Qiang Jiang , Xia Wu Multiple proteases were optimized to hydrolyze the rice residue protein (RRP) to produce novel antioxidant peptides. An antioxidant peptide fraction (RRPB3) with IC 50 of 0.25 mg/ml was purified from the RRP hydrolysate using membrane ultrafiltration followed by...
Mon, 02/16/2015 - 07:22
Journal of Agricultural and Food ChemistryDOI: 10.1021/jf505854w
Mon, 02/16/2015 - 03:20
Both deoxynivalenol (DON) and fumonisin B1 (FB1) are common contaminants of feed. Fumonisins (FBs) in general have a very limited oral bioavailability in healthy animals. Previous studies have demonstrated that chronic exposure to DON impairs the intestinal barrier function and integrity, by affecting the intestinal surface area and function of the tight junctions. This might influence the oral...
Mon, 02/16/2015 - 03:20
Contamination of crops used for food and feed production with Fusarium mycotoxins, such as deoxynivalenol (DON), raise important health and economic issues all along the food chain. Acute exposure to high DON concentrations can alter the intestinal barrier, while chronic exposure to lower doses may exert more subtle effects on signal transduction pathways, leading to disturbances in cellular...
Sun, 02/15/2015 - 01:18
Publication date: August 2015 , Volume 54 Author(s): Ksenija Markov , Branka Mihaljević , Ana-Marija Domijan , Jelka Pleadin , Frane Delaš , Jadranka Frece Detailed investigation on the effect of gamma (γ) irradiation on germination, sporulation, and growth of aflatoxigenic moulds ( Aspergillus parasiticus 2999, Aspergillus flavus 305, and Aspergillus niger 388), as well as on the...
Sun, 02/15/2015 - 01:18
Publication date: August 2015 , Volume 54 Author(s): Natalia Arroyo-Manzanares , José F. Huertas-Pérez , Ana M. García-Campaña , Laura Gámiz-Gracia In this paper, a procedure for the determination of aflatoxin B 1 , B 2 , G 1 and G 2 in five different animal feedstuffs, intended to feed different mammalians and fowls, has been developed and validated. The method is based on a very simple...
Sun, 02/15/2015 - 01:18
Publication date: August 2015 , Volume 54 Author(s): C. Chatelard-Chauvin , F. Pelissier , S. Hulin , M.C. Montel The aim of this study was to run challenge tests to investigate the behaviour of Listeria monocytogenes in raw milk Cantal type uncooked pressed cheeses throughout the cheese process and during storage in different packagings (stretch film, vacuum packs and kraft paper) at 6 °...
Sun, 02/15/2015 - 01:18
Publication date: August 2015 , Volume 54 Author(s): Saet-Byul Park , Hae-Yeong Kim , Jae-Hwan Kim A diverse range of genetic elements has been used to develop genetically modified organisms (GMOs) over the last 18 years. Screening methods that target few elements, such as the Cauliflower Mosaic Virus 35S promoter (P-35S) and Agrobacterium tumefaciens nopaline terminator (T-nos), are not...
Sun, 02/15/2015 - 01:18
Publication date: 4 May 2015 , Volume 200 Author(s): Perng-Kuang Chang , Leslie L. Scharfenstein , Cesar D. Solorzano , Hamed K. Abbas , Sui-Sheng T. Hua , Walker A. Jones , Robert M. Zablotowicz Aspergillus oryzae and Aspergillus flavus are closely related fungal species. The A. flavus morphotype that produces numerous small sclerotia (S strain) and aflatoxin has a unique 1.5 kb...
Sat, 02/14/2015 - 03:20
The feasibility of prediction of cadmium (Cd) content in brown rice was investigated by near-infrared spectroscopy (NIRS) and chemometrics techniques. Spectral pretreatment methods were discussed in detail. Synergy interval partial least squares (siPLS) algorithm was used to select the efficient combinations of spectral subintervals and wavenumbers during constructing the quantitative calibration...
Sat, 02/14/2015 - 03:20
Control of Salmonella enterica on tomatoes is important for food safety. The aim of this research was to evaluate the survival of Salmonella enterica serovars Montevideo (SM) and Typhimurium (ST) on tomatoes exposed to gaseous chlorine dioxide and Pseudomonas chlororaphis (Pc). Pc was applied to stem scars of tomatoes prior to inoculations with SM and ST. Tomatoes were treated with gaseous ClO2...
Sat, 02/14/2015 - 01:23
Piklu Roy Chowdhury, Ian G. Charles, Steven P. Djordjevic In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and...
Sat, 02/14/2015 - 01:23
Vaccines targeting mucosal immunity are important for the control of infection by pathogens with mucosal portals of entry, such as avian influenza. However, reliable and effective methods for determining levels of mucosal IgA stimulated by vaccination are not well developed in poultry and are necessary for determining efficacy. The objective of the present study was to compare different ELISA...
Sat, 02/14/2015 - 01:23
Publication date: 15 July 2015 , Volume 179 Author(s): Samir Mohammed Abd-Elghany , Khalid Ibrahim Sallam Total aflatoxins (AFT) and ochratoxin A (OTA) levels were estimated using the VICAM AflaTest and OchraTest immunoaffinity fluorometric method in a total of 50 meat products (25 each of beef luncheon and beef burger) purchased from different supermarkets in Mansoura city, Egypt. All the...
Sat, 02/14/2015 - 01:23
Staphylococcal enterotoxin B (SEB) and related exotoxins are important virulence factors produced by Staphylococcus aureus as they cause human diseases such as food poisoning and toxic shock. These toxins bind directly to cells of the immune system resulting in hyperactivation of both T lymphocytes and monocytes/macrophages. The excessive release of proinflammatory cytokines from these cells...
Fri, 02/13/2015 - 06:20
Publication date: Available online 11 February 2015 Food and Chemical Toxicology Author(s): Vesna Matović , Aleksandra Buha , Danijela Ðukić-Ćosić , Zorica Bulat Besides being important occupational hazards, lead and cadmium are nowadays metals of great environmental concern. Both metals, without any physiological functions, can induce serious adverse health effects in various organs and...
Fri, 02/13/2015 - 06:20
Biofilm formation is crucial to the environmental survival and transmission of Vibrio cholerae, the facultative human pathogen responsible for the disease cholera. During its infectious cycle V. cholerae experiences fluctuations in temperature within the aquatic environment and during the transition between human host and aquatic reservoirs. In this study, we report that biofilm formation is...
Fri, 02/13/2015 - 06:20
We report the population structure and dynamics of one E. coli population of wild mallard ducks in their natural environment over four winter seasons, following the characterization of 100 isolates each consecutive season. Macrorestriction analysis was used to define isolates variously as multi- or one-year PFGE types. Isolates were characterized genotypically based on virulence-associated genes...

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