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Research Publications from Peer-Reviewed Journals

Tue, 07/02/2019 - 20:00
Salmonella enterica serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in S. Typhimurium had been well defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In the current work, we investigated an oqxAB-positive plasmid pHXY0908 and analyzed its role in S....
Tue, 07/02/2019 - 17:00
Yasuha Arai, Norihito Kawashita, Madiha Salah Ibrahim, Emad Mohamed Elgendy, Tomo Daidoji, Takao Ono, Tatsuya Takagi, Takaaki Nakaya, Kazuhiko Matsumoto, Yohei Watanabe Avian influenza virus H9N2 has been endemic in birds in the Middle East, in particular in Egypt with multiple cases of human infections since 1998. Despite concerns about the pandemic threat posed H9N2, little is known about the...
Tue, 07/02/2019 - 12:33
Abstract The study aimed at investigating the occurrence and molecular characterization of zoonotic Anisakis larvae in imported, retail‐marketed smoked herring (Clupea harengus) and frozen mackerel (Scomber scombrus) in Egypt. A total of 154 fishes (84 ready‐to‐eat smoked herring and 70 frozen mackerel) were collected. The prevalence of Anisakis larvae Type I in the whole specimen of smoked...
Tue, 07/02/2019 - 12:33
Abstract The study aimed at investigating the occurrence and molecular characterization of zoonotic Anisakis larvae in imported, retail‐marketed smoked herring (Clupea harengus) and frozen mackerel (Scomber scombrus) in Egypt. A total of 154 fishes (84 ready‐to‐eat smoked herring and 70 frozen mackerel) were collected. The prevalence of Anisakis larvae Type I in the whole specimen of smoked...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Tue, 07/02/2019 - 12:16
Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the...
Tue, 07/02/2019 - 10:29
Because of less explored flavor characteristics and extensive utilization of bovine bone marrow extract (BBME) in food industry, this study was designed to focus on its flavor generation via Maillard reaction using different heating temperatures. Response surface method (RSM) was used to optimize the reaction conditions for generation of meaty flavor. Optimal conditions (time, temperature, and pH...
Tue, 07/02/2019 - 07:28
Abstract Nowadays, Listeria monocytogenes continues to be a major health issue. Therefore, improvements in the speed and reliability of its detection are still needed. In the present study, the combination of real‐time Recombinase Polymerase Amplification (qRPA) with immunomagnetic separation (IMS) is described. The proposed methodology was tested against a real‐time PCR method, and was...
Tue, 07/02/2019 - 05:54
Abstract Objectives This study investigates the frequency and characteristics of carbapenemase‐producing Escherichia coli/Klebsiella pneumoniae (CPE/K) and extended‐spectrum cephalosporinase‐producing E. coli/K. pneumoniae (ESCE/K) in healthy humans and livestock in rural Cambodia. Additionally, household practices as risk factors for faecal carriage of ESCE/K are identified. Methods Faecal...
Tue, 07/02/2019 - 01:02
Publication date: Available online 29 June 2019Source: LWTAuthor(s): J. Arévalo Camargo, K. Murray, K. Warriner, W. LubitzAbstractAir flow characteristics and decontamination efficacy are investigated within a forced-air ozone reactor designed to control Listeria monocytogenes on apple fruit. The commercial reactor introduced an air-ozone mix through a column of apples (540 kg contained within...
Tue, 07/02/2019 - 01:02
Available online 1 July 2019 Author(s): Marie Limoges, Catherine DonnellyAbstractThe U.S. Food and Drug Administration's (FDA) 2015 Domestic and Imported Cheese and Cheese Products Compliance Program Guidelines (CPG) (U.S. FDA, 2015) consider cheeses to be adulterated if non-toxigenic Escherichia coli levels of greater than 10 most probable number per gram (MPN/g) and less than 100 MPN/g are...
Tue, 07/02/2019 - 00:00
Toxins, Vol. 11, Pages 387: Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells Toxins doi: 10.3390/toxins11070387 Authors: Qiaoling Yuan Yancheng Jiang Ying Fan Yingfeng Ma Hongyu Lei Jianming Su Fumonisins (Fums) are types of mycotoxin that widely contaminante feed material crops, and can trigger potential biological toxicities to...
Mon, 07/01/2019 - 20:00
UK public health organisations perform routine antimicrobial susceptibility tests (ASTs) to characterise the potential for antimicrobial resistance in Salmonella enterica serovars. Genetic determinants of these r...
Mon, 07/01/2019 - 20:00
Cronobacter sakazakii is an emerging foodborne pathogen, which is linked to life-threatening infections causing septicemia, meningitis and necrotizing enterocolitis. These infections have been epidemiologically connected to ingestion of contaminated reconstituted powder infant formula. Even at low water activity C. sakazakii can survive for a long time; it is capable of protective biofilm...
Mon, 07/01/2019 - 15:04
Abstract Hepatitis E virus (HEV) is currently considered as a global health concern due to the recognition of its zoonotic transmission to humans, mainly from swine, and its association with the development of severe cases of hepatitis in human risk populations. The lack of updated data on HEV state of infection in swineherds of Argentina, and the necessity of robust technologies for its...
Mon, 07/01/2019 - 11:32
In open environments such as water, enterohemorrhagic Escherichia coli O157:H7 responds to inorganic phosphate (Pi) starvation by inducing the Pho regulon controlled by PhoB. This activates the phosphate-specific transport (Pst) system that contains a high-affinity Pi transporter. In the pst mutant, PhoB is constitutively activated and regulates the expression of genes in the Pho regulon. Here we...
Mon, 07/01/2019 - 11:32
Salmonella enterica serovar Typhimurium induces inflammatory diarrhea and bacterial uptake into intestinal epithelial cells using the Salmonella pathogenicity island 1 (SPI1) type III secretion system (T3SS). HilA activates transcription of the SPI1 structural components and effector proteins. Expression of hilA is activated by HilD, HilC, and RtsA, which act in a complex feed-forward regulatory...
Mon, 07/01/2019 - 11:00
Detection of pathogens is crucial in food production areas. While it is well established, swabbing as a state-of-the-art sampling method offers several drawbacks with respect to yield, standardization, overall handling, and long-term monitoring. This led us to develop and evaluate a method that is easier to use at a lower cost and that should be at least as sensitive. After evaluating sundry...
Mon, 07/01/2019 - 11:00
Salmonella enterica is increasingly linked to disease outbreaks associated with consumption of low-water-activity (low-aw) foods. Persistence of the pathogen in these foods was attributed to its ability to implement desiccation resistance mechanisms. Published knowledge about methods that disrupt desiccation resistance in S. enterica is lacking. We hypothesize that strong membrane-active...
Mon, 07/01/2019 - 11:00
Salmonella spp. are among the most important foodborne pathogens and the third leading cause of human death among diarrheal diseases worldwide. Animals are the primary source of this pathogen, and animal-based foods are the main transmission route to humans. Thus, understanding the global epidemiology of Salmonella serovars is key to controlling and monitoring this bacterium. In this context,...
Mon, 07/01/2019 - 11:00
To identify and obtain the indigenous degraders metabolizing phenanthrene (PHE) and biphenyl (BP) from the complex microbial community within industrial wastewater, DNA-based stable-isotope probing (DNA-SIP) and cultivation-based methods were applied in the present study. DNA-SIP results showed that two bacterial taxa (Vogesella and Alicyclobacillus) were considered the key biodegraders...
Mon, 07/01/2019 - 11:00
Bacterial adaptation is characterized by a lag phase during which cells do not multiply or modify their physiology to cope with the constraints of their environment. Our aim was to determine a sequence of events during the lag phase of growth at low temperature and pH for three Bacillus cereus strains. The onsets of expression of two genes, one of which is essential for stress adaptation (cshA,...
Mon, 07/01/2019 - 11:00
Minicircle DNA (mcDNA), which contains only the necessary components for eukaryotic expression and is thus smaller than traditional plasmids, has been designed for application in genetic manipulation. In this study, we constructed a novel plasmid containing both the Cre recombinase under the phosphoglycerate kinase (PGK) promoter and recombinant lox66 and lox71 sites located outside the...
Mon, 07/01/2019 - 11:00
Detection of salmonellae within poultry environments is an important component of many food safety programs, but sampling approaches vary greatly and may not enable the detection of salmonellae when bacteria are present at a low prevalence or concentration. Intensive longitudinal sampling within caged sheds enabled us to undertake a longitudinal analysis of the spatial distribution of salmonellae...
Mon, 07/01/2019 - 11:00
Essential oils (EOs) have demonstrated wide-spectrum antimicrobial activities and have been actively studied for their application in foods as alternative natural preservatives. However, information regarding microbial adaptive responses and changes in virulence properties following sublethal EO exposure is still scarce. The present study investigated the effect of sublethal thymol (Thy),...
Mon, 07/01/2019 - 01:08
Publication date: Available online 29 June 2019 Innovative Food Science & Emerging TechnologiesAuthor(s): Veli Gök, Simge Aktop, Mehmet Özkan, Oktay TomarAbstractIn this study the effectiveness of cold atmospheric plasma (CAP) in inactivating two bacterial pathogens on inoculated pastırma was investigated. In addition, the effect of this treatment on the total mesophilic aerobic bacteria (...
Mon, 07/01/2019 - 01:05
Available online 28 June 2019 Author(s): Jihe Zhu, Yao Chen, Chenghao Lv, Weiguo Wu, Si QinAbstractIn recent years, cadmium (Cd) contaminated rice has aroused widespread concerns about the safety of rice or its products, and microbial fermentation has been proved to be a promising method for reducing Cd. The fermented rice flour can be applied as the main ingredient of rice noodles, a traditional...
Mon, 07/01/2019 - 01:05
Available online 28 June 2019 Author(s): Hela Houissa, Salma Lasram, Michael Sulyok, Bojan Šarkanj, Angélique Fontana, Caroline Strub, Rudolf Krska, Sabine Galindo, Abdelwahed GhorbelAbstractThe current study assesses the natural occurrence of mycotoxins and fungal metabolites in Tunisian Pearl millet. Investigations using a multi-mycotoxin liquid chromatography/electrospray ionization-tandem...
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Qisen Xiang, Wenjie Wang, Dianbo Zhao, Liyuan Niu, Ke Li, Yanhong BaiAbstractThis study was designed to evaluate the synergistic antimicrobial effect of plasma-activated water (PAW) combined with mild heat (40, 50, and 60 °C) against Escherichia coli O157:H7. Populations of E. coli O157:H7 were reduced from about 8.28 log CFU/mL to undetectable levels...
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Debao Niu, Qian-Yi Wang, Er-Fang Ren, Xin-An Zeng, Lang-Hong Wang, Tian-Fu He, Qing-Hui Wen, Charles S. BrennanAbstractEugenol (2-Methoxy-4-(2-Propenyl) Phenol; EUG) is a natural antibacterial ingredient of the essential oil from many aromatic plants. The antimicrobial mechanism of EUG and its combined inactivation effect with pulsed electric fields (PEF)...
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Linlin Shang, Xuexia Bai, Chi Chen, Lijun Liu, Mingyan Li, Xiaoshuang Xia, Yun WangAbstractOchratoxin A (OTA) is a type of mycotoxin mainly produced by fungus belonging to the genus Aspergillus and Penicillium and plays a critical role in food safety since it causes serious health problem. Biological control by using microorganisms is considered a...
Mon, 07/01/2019 - 01:05
Available online 30 June 2019 Author(s): Xiuli Ma, Li Hong, Jiukai Zhang, Wensheng Huang, Jianxun Han, Yiqiang Ge, Jinlyu Sun, Ying ChenAbstractSesame (Sesamum indicum) is a well-recognized food allergen, and sesame-related allergies have been increasingly reported worldwide. Patients with sesame allergy are specifically allergic to different sesame proteins (Ses i 1–7). Because of the increasing...
Mon, 07/01/2019 - 01:05
Available online 30 June 2019 Author(s): Linshu Lan, Ru Zhang, Xianglian Zhang, Hui ShiAbstractFoodborne pathogens can survive as the state of sublethal injury in adverse environments and recover physiological function and virulence under suitable conditions. Slightly acidic electrolyzed water (SAEW) is a non-thermal treatment to decontaminate in food industry and can induce sublethal injury....
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Juliana Ramos, Juliana Mareze, Eleni Patrinou, Jesús A. Santos, Teresa-María López-DíazAbstractFifteen samples of semi-hard ripened cheeses, both spoiled (10) and unspoiled (5), and obtained from cheese factories located in Northwest of Spain, were analysed by a dilution plating technique and direct sampling. A total of 32 isolates were identified at...
Mon, 07/01/2019 - 01:05
Available online 30 June 2019 Author(s): Andrea Tarazona, José V. Gómez, Eva M. Mateo, Misericordia Jiménez, Fernando MateoAbstractCereal grains are essential ingredient in food, feed and industrial processing. One of the major causes of cereal spoilage and mycotoxin contamination is the presence of toxigenic Fusarium spp. Nanoparticles have immense applications in agriculture, nutrition,...
Mon, 07/01/2019 - 00:00
With the increasing of the food safety awareness, the quality control of amaranth as the common food additives was significant for human’s physical health. The objective of this study was to establish a green, simple, and rapid fluorescence sensor for amaranth quantification detection. The nitrogen-doped graphene quantum dots (NGQDs) were synthesized under a friendly...
Mon, 07/01/2019 - 00:00
As patulin is a kind of mycotoxin commonly found in fruit products, it is of great importance to develop simple and accurate analytical methods for the sensitive detection of patulin (PAT). In this work, based on molecular imprinted polymer (MIP) and surface-enhanced Raman scattering (SERS) technique, a specific and sensitive PAT sensor was developed. To prepare the MIP-SERS...
Mon, 07/01/2019 - 00:00
Escherichia coli O157:H7 and Salmonella choleraesuis are two important foodborne pathogens that cause illnesses and even deaths. Rapid and convenient methods, such as immunochromatographic assays (ICAs), are useful for detecting these two pathogens. Herein, we developed a highly sensitive ICA for the simultaneous quantitative analysis of these two foodborne pathogens. Silver...
Mon, 07/01/2019 - 00:00
Abstract Heavy metals, being toxic in nature, are one of the most persistent problems in wastewater. Unabated discharge of large amount of heavy metals into water bodies are known to cause several environmental and health impacts. Biological remediation processes like microbial remediation and phytoremediation are proved to be very effective in the reduction of heavy metal...
Mon, 07/01/2019 - 00:00
Abstract Peanuts with fungal contamination may contain aflatoxin, a highly carcinogenic substance. We propose the use of hyperspectral imaging to quickly and noninvasively identify fungi-contaminated peanuts. The spectral data and spatial information of hyperspectral images were exploited to improve identification accuracy. In addition, successive projection was adopted to...
Sun, 06/30/2019 - 08:13
The changes in chemical composition of jackfruit (Artocarpus heterophyllus) during different stages of maturation (stage I–stage IV) were investigated. Further, deep‐fried jackfruit chips, a very popular snack of Asian countries, was prepared and the occurrence of acrylamide in the chips was estimated and correlated with the composition. Total carotenoids increased during maturation, while...
Sun, 06/30/2019 - 07:43
Co‐encapsulation of lupulon and xanthohumol, as major bioactive components of hop in lecithin‐based nanoliposomes was developed by sonication method. The procedure of production was optimized by considering the duration and the power of ultrasound device as well as the concentration of lecithin as variables for the Response Surface Methodology. The average particle size, ζ‐potential, and the...
Sun, 06/30/2019 - 07:13
Jujube juice was separately undergoing controlled lactic acid fermentation at 37°C for 24 hr with 90 different lactic acid bacteria (LAB) strains comprising 30 Lactobacillus casei strains, 30 Lactobacillus plantarum strains, and 30 Enterococcus faecium strains. Electronic nose and Electronic tongue combined with multivariate statistical analysis methods were applied for the investigation of how...
Sun, 06/30/2019 - 01:12
Publication date: Available online 28 June 2019 Environmental ResearchAuthor(s): Iman Al-Saleh, Somiah Al-Mohawes, Reem Al-Rouqi, Rola ElkhatibAbstractA total of 206 lactating mothers and their infants (3-12 months) were included in this study to evaluate postnatal exposure to neurotoxic pollutants such as methylmercury (MeHg), lead (Pb), manganese (Mn), dichlorodiphenyltrichloroethane (DDT) and...
Sun, 06/30/2019 - 01:12
Publication date: Available online 28 June 2019 Environmental ResearchAuthor(s): Huma Ajab, Aamir Amanat Ali Khan, Muhammad Shahid Nazir, Asim Yaqub, Mohd Azmuddin AbdullahAbstractEnvironmental monitoring is important to determine the extent of eco-system pollution and degradation so that effective remedial strategies can be formulated. In this study, an environmentally friendly and cost-...

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