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Research Publications from Peer-Reviewed Journals

Wed, 07/29/2015 - 01:24
Aims To report fungal and aflatoxin contamination in stored tobacco leaves and potential of Foeniculum vulgare (fennel) seed essential oil (EO) as plant based preservative in protection of tobacco during storage. Methods and Results Mycological analysis of tobacco samples was done by surface sterilization and serial dilution tests. The Aspergillus flavus isolates were screened for their...
Wed, 07/29/2015 - 01:24
Wild animals can serve as hosts, amplifiers, or reservoirs for various zoonotic diseases. Most species of deer in highly fragmented agricultural landscapes, search out maximum cover from intrusive human activity. Hence, the likelihood of zoonosis transmission is likely to increase the more humans and wildlife interact. In our study we conducted a comparative analysis of bacteria isolated from the...
Wed, 07/29/2015 - 01:24
The aim of the study was to compare the antimicrobial activities of freshly-made, heat-treated (HT), and 14 d stored (+)-Catechin solutions with (+)-catechin flavanol isomers in the presence of copper sulphate. (+)-Catechin activity was investigated when combined with different ratios of Cu2+; 100°C heat treatment; autoclaving; and 14 d storage against Staphylococcus aureus. Cu2+-(+)-Catechin...
Wed, 07/29/2015 - 01:24
Publication date: Available online 28 July 2015 Author(s): Aminata Cumbassá, Maria J. Barahona, Mónica V. Cunha, Beatriz Azórin, Carlos Fonseca, Luís Miguel Rosalino, Jeroen Tilburg, Ferry Hagen, Ana S. Santos, Ana Botelho Coxiella burnetii is the etiological agent of Q fever or Coxiellosis, a zoonosis mainly affecting domestic ruminants. Information on the population...
Wed, 07/29/2015 - 01:24
Publication date: Available online 28 July 2015 Author(s): Devin B. Holman, Tim McAllister, Ed Topp, André-Denis G. Wright, Trevor W. Alexander Bovine respiratory disease is the major cause of morbidity and mortality in feedlot cattle. The objective of this study was to compare the nasopharyngeal bacterial microbiota of healthy cattle and cattle treated for BRD in a commercial...
Tue, 07/28/2015 - 03:20
In this study, a molecularly imprinted polymer that can selectively recognize dimethoate, isocarbophos, and methyl parathion was synthesized. Using this compound as sorbent, a new method for simultaneous determination of the three organophosphorus pesticides by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography was established....
Tue, 07/28/2015 - 03:20
In this work, a new method for the determination of 33 carbamates (including some metabolites) in different teas, chamomile, and other herbal products intended for infusions, by ultra-high-performance liquid chromatography tandem mass spectrometry, is proposed. Quick, easy, cheap, effective, rugged, and safe (QuEChERS) methodology was evaluated as sample treatment, and after...
Tue, 07/28/2015 - 03:20
In the present study, an extraction, preconcentration, and determination method has been reported for some synthetic phenolic antioxidants and bisphenol A in honey samples using dispersive liquid–liquid microextraction technique followed by gas chromatography-flame ionization detection. The main factors influencing the extraction efficiency including extractive solvent type...
Tue, 07/28/2015 - 03:20
In this study, electrothermal atomic absorption spectrometry (ETAAS) was used for determination of lead and cadmium in Macedonian white wines. Wine samples, without prior purification, were directly injected into the ETAAS system, using matrix modifiers for Pb and Cd. The standard addition method was used for quantitative analysis of Pb and Cd content. Reliability of the...
Tue, 07/28/2015 - 01:29
The objectives of this study were to detect bacteria on restaurant menus, to determine the bacterial transfer from menus to consumers' hands and to determine the survival of bacteria on menu surfaces. Local restaurant menus were sampled at different periods of operation. The average total plate count (TPC) was 28 (0–210) cfu/15 cm2 menu sampling area during “busy” periods and 15 (0–85) cfu/15 cm2...
Tue, 07/28/2015 - 01:29
This study elucidated the effect of NaCl on the thermal resistance and cell morphology of Escherichia coli strains. E. coli O157:H7 NCCP11142, E. coli O111 ATCC12795 and E. coli O26 ATCC43887 were incubated in tryptic soy broth formulated with 0% NaCl (nonhabituated) as well as broth formulated with 2% and 4% NaCl (NaCl-habituated) at 35C. Nonhabituated, 2% NaCl-habituated and 4% NaCl-habituated...
Tue, 07/28/2015 - 01:29
Mycobacterium tuberculosis (Mtb) persists inside granulomas in the human lung. Analysis of the metabolic composition of granulomas from guinea pigs revealed that one of the organic acids accumulating in the course of infection is acetate (1), which might result either from metabolism of the pathogen, or might be provided by the host himself. Our studies characterize a metabolic pathway by which...
Mon, 07/27/2015 - 03:05
Intensive animal farming could potentially lead to outbreaks of infectious diseases. Clinicians are at the forefront of detecting unusual diseases, but the lack of specificity of zoonotic disease symptoms makes this a challenging task. We evaluated patients with community-acquired pneumonia (CAP) with known and unknown aetiology in an area with a high livestock density and a potential association...
Mon, 07/27/2015 - 01:21
Publication date: February 2016 , Volume 60 Author(s): Mohamad Es'haghi Gorji, Reza Ahmadkhaniha, Mojtaba Moazzen, Masud Yunesian, Ali Azari, Noushin Rastkari The presence and amount of 15 polycyclic aromatic hydrocarbons (PAHs) were determined in four types of commonly consumed well-done grilled meat (Kebab) in Tehran restaurants. The main goal was to assess the hazard potential...
Mon, 07/27/2015 - 01:21
Publication date: February 2016 , Volume 60 Author(s): Xiaojuan Yang, Jiahui Huang, Qingping Wu, Jumei Zhang, Shengrong Liu, Weipeng Guo, Shuzhen Cai, Shubo Yu Salmonella is a leading cause of foodborne disease worldwide and ready-to-eat (RTE) foods are regarded as a major source of infection and outbreak. In recent years, the consumption of Chinese RTE foods has raised...
Mon, 07/27/2015 - 01:21
Publication date: Available online 26 July 2015 Author(s): Kun Xiong, Zhijin Chen, Chunyue Zhu, Jianhua Li, Xiaomei Hu, Xiancai Rao, Yanguang Cong Enteric fever caused by Salmonella enterica serovar Paratyphi A has progressively increased in recent years and became a global health issue. Currently licensed typhoid vaccines do not confer adequate cross-immunoprotection against S...
Sun, 07/26/2015 - 01:36
Publication date: February 2016 , Volume 60 Author(s): Peter Tölgyessy, Zuzana Miháliková A simple, non-laborious, fast, cheap, robust and environment friendly method for lipid determination in fish samples was developed. An amount of 5 g of fish tissue homogenate in a centrifuge tube was extracted with 5 mL of acetone/ethyl acetate mixture (6:4, v/v) using vigorous shaking and after...
Sun, 07/26/2015 - 01:36
Publication date: February 2016 , Volume 60 Author(s): Marcel H. Zwietering, Liesbeth Jacxsens, Jeanne-Marie Membré, Maarten Nauta, Mats Peterz Management of microbiological food safety is largely based on good design of processes, products and procedures. Finished product testing may be considered as a control measure at the end of the production process. However, testing gives...
Sun, 07/26/2015 - 01:36
Publication date: February 2016 , Volume 60 Author(s): Dong Joo Seo, Su Been Jeon, Hyejin Oh, Bog-Hieu Lee, Sook-Young Lee, Seung Hyun Oh, Ji Youn Jung, Changsun Choi Antiviral effect of 10 flavonoids against feline calicivirus (FCV) and murine norovirus (MNV) was investigated on Crandell-Reese feline kidney (CRFK) cells and RAW 264.7 cells. Each cells infected with FCV or MNV...
Sun, 07/26/2015 - 01:36
Publication date: February 2016 , Volume 60 Author(s): P.F. Ababio, K.D.A. Taylor, M. Swainson, B.A. Daramola Eleven schools in three different hygiene categories were given hygiene training as an intervention to reported low hygiene standards. Staff hygiene knowledge scores, food temperature, food service time and microbiological quality of jollof rice (cooked rice in tomato sauce...
Sun, 07/26/2015 - 01:36
Publication date: December 2015 , Volume 52 Author(s): Jana Juránková, Walter Basso, Helena Neumayerová, Anita Frencová, Vojtech Baláž, Peter Deplazes, Břetislav Koudela Undercooked lamb and mutton are common sources of Toxoplasma gondii infection for humans. A sequence specific magnetic capture technique in combination with quantitative real-time PCR targeting the 529 bp repeat...
Sat, 07/25/2015 - 03:20
Ochratoxin A (OTA), a potentially carcinogenic mycotoxin which contaminates grains, is produced by several Aspergillus species. A comparative sequence analysis of the OTA-producing Aspergillus ochraceus fc-1 strain and other Aspergillus species was performed. Two new OTA-related polyketide synthase (PKS) (AoOTApks) genes were identified. The predicted amino acid sequence of AoOTApks-1 displayed...
Sat, 07/25/2015 - 03:20
In the scientific field, there is a progressive awareness about the potential implications of food processing on mycotoxins especially concerning thermal treatments. High temperatures may cause, in fact, transformation or degradation of these compounds. This work is aimed to study the fate of mycotoxins during bakery processing, focusing on deoxynivalenol (DON) and deoxynivalenol-3-glucoside (...
Sat, 07/25/2015 - 01:22
Publication date: February 2016 , Volume 60 Author(s): Samantha Shinbaum, Philip G. Crandall, Corliss A. O'Bryan The signing of the Food and Drug Administration's (FDA) Food Safety Modernization Act (FSMA) into law in 2011 elevated the FDA into a significantly higher, international operational role to implement a new, prevention-oriented, systems-wide approaches to food safety for the...
Sat, 07/25/2015 - 01:22
Publication date: February 2016 , Volume 60 Author(s): J.M.P.J. Garrido, V. Rahemi, F. Borges, C.M.A. Brett, E.M.P.J. Garrido A sensitive electrochemical sensor was successfully developed based on a glassy carbon (GC) electrode modified by a combination of multi-walled carbon nanotubes (MWCNT) with β-cyclodextrin (β-CD) incorporated in a polyaniline film, and applied to detect and...
Sat, 07/25/2015 - 01:22
Publication date: February 2016 , Volume 60 Author(s): Lingling Wang, Minhui Zhang, Danfeng Zhang, Lei Zhang Solid-phase extraction preconcentration system using carbon nanofibers (CNFs) packed microcolumn was developed for the simultaneous determination of the fungicides carbendazim (MBC) and thiabendazole (TBZ) by high performance liquid chromatography (HPLC). CNFs exhibited a good...
Fri, 07/24/2015 - 21:37
Niladri Sekhar Chatterjee, Kaushik Banerjee, Sagar Utture, Narayan Kamble, B. Madhusudana Rao, Satyen Kumar Panda, Suseela Mathew
Fri, 07/24/2015 - 03:20
As it became evident recently, extracellular DNA could be a versatile nutrient source of the facultative pathogen Vibrio cholerae along the different stages of its life cycle. By the use of two extracellular nucleases and periplasmic phosphatases, V. cholerae degrades extracellular DNA to nucleosides. In this study, we investigated the nucleoside uptake via identification and characterization of...
Fri, 07/24/2015 - 03:20
Background The prion protein-encoding gene (PRNP) is one of the major determinants for scrapie occurrence in sheep and goats. However, its effect on bovine spongiform encephalopathy (BSE) transmission to goats is not clear. Methods Goats harboring wild-type, R/Q211 or Q/K222 PRNP genotypes were orally inoculated with a goat-BSE isolate to assess their relative susceptibility to BSE infection...
Fri, 07/24/2015 - 03:20
Background Cell-culture-derived (CC) influenza vaccine production methods could provide benefits over classical embryonated-egg technology, including a higher production capacity and the faster creation of a supply that meets demand. Methods A CC-inactivated split-virus influenza A/Indonesia/5/2005(H5N1) vaccine derived from the EB66 cell line (hereafter, "CC-H5N1") was investigated in a...
Fri, 07/24/2015 - 01:30
Publication date: Available online 23 July 2015 Author(s): Byoung-Kwon Hahm, Hyochin Kim, Atul K. Singh, Arun K. Bhunia The bottleneck for accurate detection of foodborne pathogens is separation of target analytes from complex food matrices. Currently used sample preparation methods are cumbersome, arduous and lengthy; thus, a user-friendly system is desirable. A hand-held sample...
Thu, 07/23/2015 - 03:20
Publication date: Available online 22 July 2015 Author(s): Abhishek Jaiswal, Sounak Sarkar, Parijat Das, Suman Nandy, Hemanta Koley, Banwarilal Sarkar Here we describe the international scenario of Vibrio cholerae with a comparative analysis of different aspects of typing. Representative V. cholerae strains ( n = 108) associated with endemic cholera regions from 29 states of...
Thu, 07/23/2015 - 01:23
Sinéad Murphy, Jonathan L Barber, Jennifer A. Learmonth, Fiona L. Read, Robert Deaville, Matthew W. Perkins, Andrew Brownlow, Nick Davison, Rod Penrose, Graham J. Pierce, Robin J. Law, Paul D. Jepson Reproductive failure in mammals due to exposure to polychlorinated biphenyls (PCBs) can occur either through endocrine disrupting effects or via immunosuppression and increased disease risk. To...
Thu, 07/23/2015 - 01:23
David M. Greenberg, Simon Baron-Cohen, David J. Stillwell, Michal Kosinski, Peter J. Rentfrow Why do we like the music we do? Research has shown that musical preferences and personality are linked, yet little is known about other influences on preferences such as cognitive styles. To address this gap, we investigated how individual differences in musical preferences are explained by the...
Wed, 07/22/2015 - 03:20
Glycosylation is an important plant defense mechanism and conjugates of Fusarium mycotoxins often co-occur with their parent compounds in cereal-based food and feed. In case of deoxynivalenol (DON), deoxynivalenol-3-O-β-D-glucoside (D3G) is the most important masked mycotoxin. The toxicological significance of D3G is not yet fully understood so that it is crucial to obtain this compound in pure...
Wed, 07/22/2015 - 03:20
Publication date: Available online 21 July 2015 Author(s): Dov B. Schlisselberg, Edna Kler, Guy Kisluk, Dina Shachar, Sima Yaron Recent studies offer contradictory findings about the role of multidrug efflux pumps in bacterial biofilm development. Thus, the aim of this study was to investigate the involvement of the AcrAB efflux pump in biofilm formation by investigating the...
Wed, 07/22/2015 - 01:27
Phenotypic heterogeneity describes the occurrence of "nonconformist" cells within an isogenic population. The nonconformists show an expression profile partially different from that of the remainder of the population. Phenotypic heterogeneity affects many aspects of the different bacterial lifestyles, and it is assumed that it increases bacterial fitness and the chances for survival of the whole...
Wed, 07/22/2015 - 01:27
Cronobacter species are Gram-negative opportunistic pathogens that can cause serious infections in neonates. The lipopolysaccharides (LPSs) that form part of the outer membrane of such bacteria are possibly related to the virulence of particular bacterial strains. However, currently there is no clear overview of O-antigen diversity within the various Cronobacter strains and links with virulence....
Wed, 07/22/2015 - 01:27
Cronobacter spp. (formerly defined as Enterobacter sakazakii) are opportunistic bacterial pathogens of both infants and adults. In this study, we analyzed 70 Cronobacter isolates from powdered infant formula (PIF) and an infant formula production facility in China to determine possible contamination routes. The strains were profiled by multilocus sequence typing (MLST), pulsed-field gel...
Wed, 07/22/2015 - 01:27
Campylobacter jejuni is a common cause of the frequently reported food-borne diseases in developed and developing nations. This study describes the development of multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) using capillary electrophoresis as a novel typing method for microbial source tracking and epidemiological investigation of C. jejuni. Among 36 tandem repeat loci...
Wed, 07/22/2015 - 01:27
Taxonomic classification of Clostridium botulinum is based on the production of botulinum neurotoxin (BoNT), while closely related, nontoxic organisms are classified as Clostridium sporogenes. However, this taxonomic organization does not accurately mirror phylogenetic relationships between these species. A phylogenetic reconstruction using 2,016 orthologous genes shared among strains of C....

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