Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Tue, 08/09/2016 - 02:01
This study developed a new method to extract abalone visceral polysaccharide (AVP) and remove its heavy metals (except for mercury) by combining enzymatic hydrolysis, plate frame filtration, alcohol precipitation, high activated clay absorption with cation exchange resin and anion exchange resin. Alcohol precipitation, dialysis, activated carbon adsorption and high activated clay adsorption were...
Tue, 08/09/2016 - 01:20
Raikamal Ghosh, Naresh C. Sharma, Kalpataru Halder, Rupak K. Bhadra, Goutam Chowdhury, Gururaja P. Pazhani, Sumio Shinoda, Asish K. Mukhopadhyay, G. Balakrish Nair, Thadavarayan Ramamurthy
Tue, 08/09/2016 - 00:00
Abstract The increasing use of pesticides for boosting the yield of agricultural crops also impart toxic residues which ultimately extend to numerous physiological disorders upon consumption. The present study was designed as an effort to assess the reduction potential of various chemical solutions and to minimize the pesticide residues in cauliflower (Brassica oleracea var....
Mon, 08/08/2016 - 17:00
Marta Isabel Gracia, Jaime Sánchez, Carlos Millán, Óscar Casabuena, Peter Vesseur, Ángel Martín, Francisco Javier García-Peña, Pedro Medel Two independent trials were carried out to evaluate the effect of feed form, whole wheat (WW) and oat hulls (OH) addition on gastrointestinal (GIT) weight and Campylobacter jejuni colonization in orally infected birds. In Trial 1, there were six treatments...
Mon, 08/08/2016 - 17:00
Bo Cui, Lei Feng, Chunxin Wang, Dongsheng Yang, Manli Yu, Zhanghua Zeng, Yan Wang, Changjiao Sun, Xiang Zhao, Haixin Cui Poorly water-soluble compounds are difficult to develop as pesticide products and face great challenges in water-based and environmentally friendly formulation development. In this study, high pressure homogenization combined with lyophilization was adopted to prepare the...
Mon, 08/08/2016 - 17:00
Mako Yamamoto, Ming Zhao, Yukihiko Hiroshima, Yong Zhang, Elizabeth Shurell, Fritz C. Eilber, Michael Bouvet, Makoto Noda, Robert M. Hoffman Tumor-targeting Salmonella enterica serovar Typhimurium A1-R (Salmonella A1-R) had strong efficacy on a melanoma patient-derived orthotopic xenograft (PDOX) nude-mouse model. GFP-expressing Salmonella A1-R highly and selectively colonized the PDOX melanoma...
Mon, 08/08/2016 - 13:02
The Twin-arginine translocation (Tat) system mediates secretion of folded proteins that in bacteria, plants and archaea are identified via an N-terminal signal peptide. Tat systems are associated with virulence in many bacterial pathogens and our previous studies revealed that Tat deficient Yersinia pseudotuberculosis was severely attenuated for virulence. Aiming to identify Tat-dependent...
Mon, 08/08/2016 - 11:06
Abstract Dysbiosis of intestinal microbiota and hyperactive immune responses seem to be crucial for the uncontrolled inflammation in inflammatory bowel diseases (IBD). Modulation of the microbiome and immune stimulation of the intestinal epithelium were suggested as therapeutic approaches. In this study live attenuated and dead bacterial cells of Salmonella Typhimurium SL7207 – a widely used...
Mon, 08/08/2016 - 09:06
Abstract AimsTo identify faecal pollution along the southeastern Florida coast and determine the performance of a reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) method for pepper mild mottle virus (PMMoV). Methods and ResultsIn 2014, bimonthly surface water samples were collected from inlets, exposed to runoff and septic seepage, and coastal sites, exposed to ocean...
Mon, 08/08/2016 - 04:51
Publication date: October 2016 , Volume 236 Author(s): Robert M. Lawrence, James D. Zook, Brenda G. Hogue Inherent in the study of viruses is the risk of pathogenic exposure, which necessitates appropriate levels of biosafety containment. Unfortunately, this also limits the availability of useful research instruments that are located at facilities not equipped to handle infectious...
Mon, 08/08/2016 - 00:00
Wine and fermenting musts are grape products widely consumed worldwide. Since the presence of mycotoxin-producing fungi may greatly compromise their quality characteristics and safety, there is an increasing need for relatively rapid “user friendly” quantitative assays to detect fungal contamination both in grapes delivered to wineries and in final products. Although other fungi are most...
Mon, 08/08/2016 - 00:00
Abstract A ready-to-eat food product in the form of compressed bar from beetroot, enriched with dates and oats was developed. Levels of intermediate moisture beetroot shreds (60–100 g), dates (10–30 g) and oats (5–15 g) were optimized using response surface methodology and its quality attributes were investigated over a period of 6 months at different temperature conditions (...
Sat, 08/06/2016 - 06:42
Abstract Shoe soles are possible vectors for infectious diseases. Although studies have been performed to assess the prevalence of infectious pathogens on shoe soles and decontamination techniques, no systematic review has ever occurred. The aim of this study was to perform a systematic review of the literature to determine the prevalence of infectious agents on shoe bottoms and possible...
Sat, 08/06/2016 - 05:15
Sous vide cook-chill (SVCC) is characterized by vacuum-packaging raw or partially prepared foods before pasteurization, followed by rapid chilling and storage below 3C. The application of essential oils (EOs) to food products is a suitable strategy to control pathogens and to extend their shelf life by reducing microbial levels and oxidative processes. The aim of this study was to evaluate the...
Fri, 08/05/2016 - 17:00
Emilie Lang, Fiona Zoz, Cyril Iaconelli, Stéphane Guyot, Pablo Alvarez-Martin, Laurent Beney, Jean-Marie Perrier-Cornet, Patrick Gervais Drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. The aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary...
Fri, 08/05/2016 - 02:31
ScopeThis study investigated the effects of cooked whole asparagus (ASP) versus its equivalent level of purified flavonoid glycoside, rutin (RUT), on dextran sodium sulfate (DSS)-induced colitis and subsequent colitis recovery in mice. Methods and resultsC57BL/6 male mice were fed an AIN-93G basal diet (BD), or BD supplemented with 2% cooked ASP or 0.025% RUT for 2 wks prior to and during colitis...
Fri, 08/05/2016 - 02:00
Summary Using data collected from a cross-sectional study of 25 farms (eight beef, eight swine and nine dairy) in 2010, we assessed clustering of molecular subtypes of C. jejuni based on a Campylobacter-specific 40 gene comparative genomic fingerprinting assay (CGF40) subtypes, using unweighted pair-group method with arithmetic mean (UPGMA) analysis, and multiple correspondence analysis. Exact...
Fri, 08/05/2016 - 01:01
European Commission Regulation (EU) 1276/2011 requires that fishery products intended for raw consumption be frozen at −20°C for not less than 24 h or at −35°C for at least 15 h in order to kill viable parasites other than trematodes. But because marinating processes are not always effective in destroying nematode larvae, raw marinated fish preparations should be frozen before consumption. This...
Fri, 08/05/2016 - 01:01
The consumption of fruits and vegetables is increasing worldwide because of the positive impact of these foods on human health. Ready-to-eat, raw whole, and frozen fruits and vegetables were purchased from markets and examined for the presence of nontuberculous mycobacteria (NTM) using culture, real-time PCR (qPCR), and sequencing. Using qPCR, Mycobacterium sp. at 100 to 104 ge/g (genome...
Fri, 08/05/2016 - 01:01
This study determined the prevalence of multidrug-resistant (MDR) Acinetobacter baumannii on fresh vegetables collected from farmers' markets in Connecticut. One hundred samples each of fresh carrots, potatoes, and lettuce were sampled and streaked on selective media, namely Leeds Acinetobacter and MDR Acinetobacter agars. All morphologically different colonies from MDR Acinetobacter agar were...
Fri, 08/05/2016 - 01:01
In recent years, tree fruits have emerged as a new concern for Listeria monocytogenes contamination. The objective of the current study was to evaluate the potential internalization of L. monocytogenes from the surface of avocados into the edible portions of the fruit during certain postharvest practices simulated in a laboratory setting. One set of intact avocados was spot inoculated with L....
Fri, 08/05/2016 - 01:01
Many consumers do not practice proper food safety behaviors when preparing food in the home. Several approaches have been taken to improve food safety behaviors among consumers, but there still is a deficit in actual practice of these behaviors. The objective of this study was to assess whether the introduction of food safety instructions in recipes for chicken breasts and ground turkey patties...
Fri, 08/05/2016 - 01:01
The blue discoloration in Mozzarella cheese comes from bacterial spoilage due to contamination with Pseudomonas. Fourteen Pseudomonas fluorescens strains from international collections and 55 new isolates of dominant bacterial populations from spoiled fresh cheese samples were examined to assess genotypic and phenotypic strain diversity. Isolates were identified by 16S rRNA gene sequencing and...
Fri, 08/05/2016 - 01:01
We compared the prevalence of antibiotic-resistant Escherichia coli isolates from household-level producers of broiler (commercial source breeds) and local chickens in the Arusha District of Tanzania. Households were composed of a single dwelling or residence with independent, penned broiler flocks. Free-range, scavenging chickens were mixed breed and loosely associated with individual households...
Fri, 08/05/2016 - 01:01
The main purpose of this study was to determine the prevalence of Escherichia coli O157 on bovine carcasses before and after chilling at a large slaughterhouse located in the city of Barcelona, Spain, to assess the effectiveness of dry chilling on reducing E. coli O157 contamination of carcasses. In addition, the study characterized the E. coli O157 strains isolated in terms of virulence factors...
Fri, 08/05/2016 - 01:01
To extend the shelf life of apples in South Korea, we evaluated the effect of gamma irradiation alone or gamma irradiation combined with fumigation on the control of postharvest decay caused by Botrytis cinerea and Monilinia fructigena. An irradiation dose of 1.0 kGy caused the maximal inhibition of B. cinerea and M. fructigena spore germination. The gamma irradiation dose required to reduce the...
Fri, 08/05/2016 - 01:01
First, the antibacterial, antibiofilm effect and chemical composition of burdock (Arctium lappa L.) leaf fractions were studied. Then, the efficiency of burdock leaf fractions in pork preservation was evaluated. The results showed that burdock leaf fraction significantly inhibited the growth and biofilm development of Escherichia coli and Salmonella Typhimurium. MICs of burdock leaf fractions on...
Fri, 08/05/2016 - 01:01
A reduced-sodium ready-to-eat (RTE) uncured turkey was manufactured with buffered dry vinegar treatments to validate the inhibition of Listeria monocytogenes and spoilage microflora and to determine the effects on sensory and quality attributes. Samples were stored at 4°C for 12 weeks, and the study was independently replicated three times. Two different five-strain inocula of L. monocytogenes...
Fri, 08/05/2016 - 01:01
Staphylococcal food poisoning (SFP) is an important foodborne disease worldwide, and milk and milk products are commonly associated with SFP outbreaks. The objectives of this study were to investigate the distribution of staphylococcal enterotoxin (se) genes in Staphylococcus aureus from raw cow's milk and milk products and to assess their genetic background with the spa typing method. Of the 549...
Fri, 08/05/2016 - 01:01
An effective and rapid method for the separation of bacteria from food matrix remains a bottleneck for rapid bacteria detection for food safety. Bacteria can strongly attach to a food surface or internalize within the matrix, making their isolation extremely difficult. Traditional methods of separating bacteria from food routinely involve stomaching, blending, and shaking. However, these methods...
Fri, 08/05/2016 - 01:01
Vibrio parahaemolyticus can cause serious human seafoodborne gastroenteritis and even death. In this study, we isolated and characterized 208 V. parahaemolyticus strains from 10 species of commonly consumed crustaceans and shellfish available in fish markets in Shanghai, People's Republic of China, in 2014. Most of these aquatic species had not been detected previously. The results revealed an...
Fri, 08/05/2016 - 01:01
The disinfectant and antimicrobial susceptibility profiles of 138 non-O157 Shiga toxin–producing Escherichia coli strains (STECs) from food animals and humans were determined. Antimicrobial resistance (AMR) was moderate (39.1% of strains) in response to 15 antimicrobial agents. Animal strains had a lower AMR prevalence (35.6%) than did human strains (43.9%) but a higher prevalence of the...
Fri, 08/05/2016 - 01:01
The objective of this study was to determine antimicrobial resistance and elucidate the resistance mechanism in nontyphoidal Salmonella enterica serovars isolated from food products imported into the United States from 2011 to 2013. Food products contaminated with antimicrobial-resistant nontyphoidal S. enterica were mainly imported from Taiwan, Indonesia, Vietnam, and China. PCR, DNA sequencing...
Fri, 08/05/2016 - 01:01
During site visits of veal processors, the U.S. Department of Agriculture, Food Safety Inspection Service (FSIS) has reported processing deficiencies that likely contribute to increased levels of veal contamination. Here, we report the results of measuring aerobic plate count bacteria (APC), Enterobacteriaceae, coliforms (CF), and Escherichia coli during eight sample collections at five veal...
Fri, 08/05/2016 - 01:01
Asymptomatic Salmonella carriage in beef cattle is a food safety concern, and the beef feedlot environment may function as a reservoir of this pathogen. The goal of this study was to identify and isolate Salmonella and Salmonella bacteriophages from beef cattle feedlot environments in order to better understand the microbial ecology of Salmonella and identify phages that might be useful as anti-...
Fri, 08/05/2016 - 01:01
The objective of this study was to determine the immediate source of Escherichia coli on beef trimmings produced at a large packing plant by analyzing the E. coli on trimmings at various locations of a combo bin filled on the same day and of bins filled on different days. Ten 2,000-lb (907-kg) combo bins (B1 through B10) of trimmings were obtained from a large plant on 6 days over a period of 5...
Fri, 08/05/2016 - 01:01
Surface structure and biochemical characteristics of bacteria and produce play a major role in how and where bacteria attach, complicating decontamination treatments. Whole cantaloupe rind surfaces were inoculated with Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes at 107 CFU/ml. Average population size of Salmonella, Escherichia coli O157:H7, and L. monocytogenes recovered...
Fri, 08/05/2016 - 01:01
After harvest, pistachios are hulled with mechanical abrasion and then separated in a float tank containing water; the nuts that float (~15%; floaters) and those that sink (~85%; sinkers) are dried and stored separately. To determine the prevalence of Salmonella in pistachios, a total of 3,966 samples (1,032 floaters and 2,934 sinkers) were collected within 4 months of the 2010, 2011, and 2012...
Fri, 08/05/2016 - 00:00
To the Editor: Carbapenems, antimicrobial drugs of last resort, are recommended only for severe community- and healthcare-associated multidrug-resistant bacterial infections. In Canada, carbapenem-resistant infection rates in hospitals remained low (<0.25 cases/1,000 patient admissions) over 5 years’ (2009–2014) surveillance (1). Carbapenemase-producing bacteria have rarely been detected in...
Thu, 08/04/2016 - 23:02
Publication date: Available online 4 August 2016 Food and Chemical Toxicology Author(s): Ricardo Assunção, Carla Martins, Didier Dupont, Paula Alvito Patulin (PAT) and ochratoxin A (OTA) are well known enteropathogenic mycotoxins that are present in several foodstuffs. Processed cereal-based foods are among the first solid foods eaten by children, a particularly vulnerable population...
Thu, 08/04/2016 - 21:09
Ochratoxin A (OTA) is a toxic secondary metabolite produced by the fungi of Aspergillus and Penicillium species. Data indicate a frequent OTA contamination of cereals and cereal products, and consequently also the contamination of meat and meat products. The aim of this study was to determine a possible level of meat product consumers’ exposure to OTA through the consumption of dry-cured and...
Thu, 08/04/2016 - 18:45
Summary AtxA is a critical transcriptional regulator of plasmid-encoded virulence genes in Bacillus anthracis. Bacillus cereus G9241, which caused an anthrax-like infection, has two virulence plasmids, pBCXO1 and pBC210, that each harbor toxin genes and a capsule locus. G9241 also produces two orthologs of AtxA: AtxA1, encoded on pBCXO1, and AtxA2, encoded on pBC210. The amino acid sequence of...
Thu, 08/04/2016 - 17:00
Ahmed Abdelfattah, Michael Wisniewski, Maria Giulia Li Destri Nicosia, Santa Olga Cacciola, Leonardo Schena An amplicon metagenomic approach based on the ITS2 region of fungal rDNA was used to identify the composition of fungal communities associated with different strawberry organs (leaves, flowers, immature and mature fruits), grown on a farm using management practices that entailed the...
Thu, 08/04/2016 - 17:00
Marcelo Mendonça, Gustavo Marçal Schmidt Garcia Moreira, Fabricio Rochedo Conceição, Michael Hust, Karla Sequeira Mendonça, Ângela Nunes Moreira, Rodrigo Correa França, Wladimir Padilha da Silva, Arun K. Bhunia, José Antonio G. Aleixo Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased...
Thu, 08/04/2016 - 12:31
Competitive exclusion (CE) cultures, given as a single dose on the day of hatch, together with good hygienic practices has been shown to be a novel approach to control Salmonella in poultry. The ability of the CE product Broilact and 2 probiotics, FloraMax-B11 and Colostrum, to prevent Salmonella colonization in newly hatched chickens was evaluated employing a slightly modified Mead-model chicken...
Thu, 08/04/2016 - 12:31
Avian influenza virus subtypes H5, H7, and H9 related epidemics are accountable for huge losses to the poultry and avian industry in Pakistan. Well-timed and accurate diagnosis of subtype(s) concomitant with a specific epidemic allows a margin of prophylaxis to farmers through requisite vaccination. The current study was designed to develop and validate a rapid, accurate, and efficient diagnostic...
Thu, 08/04/2016 - 12:11
Publication date: 7 November 2016 , Volume 236 Author(s): María-Elena Álvarez-Suárez, Andrés Otero, María-Luisa García-López, Ghizlane Dahbi, Miguel Blanco, Azucena Mora, Jorge Blanco, Jesús A. Santos The aim of this study was to characterize a collection of 44 Shiga toxin-producing (STEC) and enteropathogenic Escherichia coli (EPEC) isolated from goat milk and goat farm...
Thu, 08/04/2016 - 12:11
Publication date: 7 November 2016 , Volume 236 Author(s): M. Mosquera-Fernández, P. Sanchez-Vizuete, R. Briandet, M.L. Cabo, E. Balsa-Canto This work shows that the combination of two-dimensional (2D) and three-dimensional (3D) analyses of images acquired by confocal laser scanning microscopy facilitates the quantitative spatiotemporal characterization of architectures formed by...

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