Research Publications (Food Safety)

Salmonella enterica (S. enterica) is an important foodborne pathogen, causing food poisoning and human infection, and critically threatening food safety and public health. Salmonella typing is essential for bacterial identification, tracing, epidemiological investigation, and monitoring. Serotyping and multilocus sequence typing (MLST) analysis are standard bacterial typing methods despite the low resolution.

Objectives: Ceftazidime–avibactam is a novel synthetic beta-lactam + beta-lactamase inhibitor combination. We evaluated the performance of the gradient diffusion strip method and the disk diffusion method for the determination of ceftazidime–avibactam against Enterobacterales and Pseudomonas aeruginosa.

Along with banning antibiotics, necrotic enteritis (NE), especially subclinical enteritis (SNE), poses a significant threat to the chicken industry; however, probiotics are a potentially promising intervention. We aimed to investigate the beneficial effects of Bacillus subtilis DSM29784 (BS) on the treatment of Clostridium perfringens (CP)-induced SNE in broilers.

Cadmium (Cd) is carcinogenic to humans and can accumulate in the liver, kidneys, and bones. There is widespread presence of cadmium in the environment as a consequence of anthropogenic activities. It is important to detect cadmium in the environment to prevent further exposure to humans. Previous whole-cell biosensor designs were focused on single-sensing constructs but have had difficulty in distinguishing cadmium from other metal ions such as lead (Pb) and mercury (Hg).

Background

There is a paucity of studies using clinical characteristics and whole-genome sequencing together to fully identify the risk factors of patients with Klebsiella pneumoniae (KP) bloodstream infection (BSI).

Methods

Background Antibiotic overprescribing in long-term care settings is driven by prescriber preferences and is associated with preventable harms for residents. We aimed to determine whether peer comparison audit and feedback reporting for physicians reduces antibiotic overprescribing among residents.

Background Bancroftian filariasis remains endemic in Fiji despite >10 years of mass drug administration (MDA) using diethylcarbamazine and albendazole (DA). The addition of ivermectin to this combination (IDA) has improved efficacy of microfilarial clearance at 12 months in individually randomized trials in nocturnal transmission settings, but impact in a setting of diurnally subperiodic filarial transmission has not been evaluated.

by Aurore Demars, Armelle Vitali, Audrey Comein, Elodie Carlier, Abdulkader Azouz, Stanislas Goriely, Justine Smout, Véronique Flamand, Mégane Van Gysel, Johan Wouters, Jan Abendroth, Thomas E. Edwards, Arnaud Machelart, Eik Hoffmann, Priscille Brodin, Xavier De Bolle, Eric Muraille

Salmonella is a foodborne pathogen that has contributed to numerous food safety accidents worldwide, making it necessary to detect contamination at an early stage. A pair of specific primers based on the invA gene of Salmonella was designed for PCR. Target double-stranded DNA (dsDNA) from PCR was purified and denatured at high temperature to obtain target single-stranded DNA (ssDNA).

Author(s): Yuan Fang, Jeyachchandran Visvalingam, Peipei Zhang, Xianqin Yang

Author(s): Utkarsh Jain, Kirti Saxena, Vinita Hooda, Sapna Balayan, Amar Pal Singh, Mayukh Tikadar, Nidhi Chauhan

Author(s): Rajae Zahli, Ann Karolin Scheu, Jamal Abrini, José L. Copa-Patiño, Amajoud Nadia, Skali Senhaji Nadia, Juan Soliveri

Salmonellosis is one of the most common foodborne illnesses in the world. The irrational use of antibiotics in medicine and in animal nutrition has greatly favored the emergence and spread of resistant strains of non-typhoid Salmonella.

Author(s): Rhuana S. Amaral, Joice F. Freitas, Mayana R.S. Ribeiro, Denise C. Cara Machado, Fernanda F. Rocha, Marcia C.A. Teixeira, Valbert N. Cardoso, Maria E.R. Andrade, César A. Vilela Silva, Marcelo V. Caliari, Maria A. Gomes

Latin America with its considerable North-South extent is subject to climate that varies from tropical, subtropical and warm temperate to temperate. Different agricultural products are produced in the area including cereals, oilseeds, beans, fruits and nuts together with animal production including cattle for beef and milk, pigs, poultry and fish. The heterogeneity of agriculture in Latin America is reflected in the diversity of the region’s farm structures.

Salmonella spp. is one of the primary pathogens that cause foodborne diseases worldwide. In the present study, we deeply characterized Salmonella spp. originated from foods related to human salmonellosis outbreaks in Minas Gerais – Brazil, from 2003 to 2017. In this regard, the serotyping, antimicrobial susceptibility, virulence genes and genetic polymorphism determined by rep-PCR were performed in 70 Salmonella spp. isolates. Thirteen serotypes of Salmonella spp. were identified, and S.

The presence of pesticide residues in fruit has been of extensive concern worldwide. In this paper, pesticide residues in litchi samples in China and their dietary exposure risks were evaluated. Fifty-seven pesticides in 150 litchi samples were measured by GC and UPLC/MS-MS. Seventeen different pesticides were detected and 70.7% of samples contained one or more pesticide residues.

The aim of this study was to assess the presence and antimicrobial susceptibility profile of the molecularly serogrouped Listeria monocytogenes isolates in different animal origin food products, collected from a county situated in the historical region of Transylvania, Central Romania. A total of 7.7% (17/221) of the screened samples were positive for L.

Nanozymes as a cost-effective and robust enzyme mimic have attracted widespread attention in the development of novel analytical methods. Herein, a new nanozyme-enhanced surface-enhanced Raman scattering (SERS) immunoassay platform was successfully developed using a peroxidase-mimicking nanozyme to replace the natural enzymes as a catalytic label of the enzyme-linked immunosorbent assay for the detection of allergy proteins.

Pancreatic necrosis infection (PNI) accounts for about 20–40 % of severe acute pancreatitis. PNI caused by anaerobic bacteria is unusual but when they present, Clostridium perfringens is the microorganism most commonly involved.

Abstract

Aims

To investigate phylogenetic and phenotypic diversity of resident chickpea-nodulating rhizobia from Australian cropping soils.

In the coastal countries of Southeast Asia, fish is a staple diet and certain fish species are food delicacies to local populations or commercially important to individual communities. Although there have been several suspected cases of ciguatera fish poisoning (CFP) in Southeast Asian countries, few have been confirmed by ciguatoxins identification, resulting in limited information for the correct diagnosis of this food-borne disease.

The yellow peach (Amygdalus persica), an important fruit in China, is highly susceptible to infection by Alternaria sp., leading to potential health risks and economic losses. In the current study, firstly, yellow peaches were artificially inoculated with Alternaria alternate.

The major causal agents Fusarium graminearum (F. graminearum) and Fusarium asiaticum could produce multiple mycotoxins in infected wheat, which threatens the health of humans and animals. Specifically, deoxynivalenol (DON) and its derivatives 3- and 15-acetyldeoxynivalenol (3-ADON and 15-ADON) are commonly detected mycotoxins in cereal grains. However, the good chromatographic separation of 3-ADON and 15-ADON remains challenging.

Streptococcus agalactiae, a Gram-positive bacterium, causes invasive infection known as Group B streptococcal disease (GBS). It is a leading cause of neonatal death and complications prior to delivery. The burden of GBS is unknown in India despite the high incidence of preterm and stillbirths.

Background

The incidence of invasive candidiasis is increasing worldwide. However, the epidemiology, antifungal susceptibility, and virulence of Candida spp. in most hospitals remain unclear. This study aimed to evaluate invasive candidiasis in a tertiary care hospital in Nanchang City, China.

Methods