Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Thu, 09/29/2016 - 09:55
Abstract AimsDevelopment of Taqman MGB real-time PCR (q-PCR) assays for the quantitative detection of virulence factor genes in pure culture and food samples with regards to food safety assessment. Methods and resultsNew Taqman primers and probes were designed for the ace, esp and gelE genes based on the determinants of virulence profiles of enterococcal strains from GenBank. The high specificity...
Wed, 09/28/2016 - 19:06
Abstract The increased demand for low-sodium ready-to-eat (RTE) meat products highlights the need for new strategies to ensure food safety. The application of essential oils (EOs) as natural antimicrobials in the meat industry has been suggested to prevent or control cross-contamination during meat processing operations. This work aims to quantify and model the transfer of Salmonella Enteritidis...
Wed, 09/28/2016 - 18:51
Publication date: 5 December 2016 , Volume 238 Author(s): Michal Slany, Nikol Reslova, Vladimir Babak, Alena Lorencova Toxoplasmosis is a major public health issue, due to the presence of Toxoplasma gondii, mainly in pork. The aim of this study was to determine the occurrence of T. gondii in pigs and wild boars bred in different production systems in the Czech Republic using ELISA and...
Wed, 09/28/2016 - 17:00
Man Su Kim, Eung Seo Koo, Yong Seon Choi, Ji Young Kim, Chang Hoon Yoo, Hyun Jin Yoon, Tae-Ok Kim, Hyun Bae Choi, Ji Hoon Kim, Jong Deok Choi, Kwon-Sam Park, Yongsik Shin, Young-Mog Kim, GwangPyo Ko, Yong Seok Jeong The presence of human norovirus in the aquatic environment can cause outbreaks related to recreational activities and the consumption of norovirus-contaminated clams. In this study,...
Wed, 09/28/2016 - 04:27
Abstract A semi-quantitative ecometric technique was used to evaluate efficacy of selective culture media. It involved a standardized chronological streaking technique, leading to ever-decreasing number of colonies. The objective was to evaluate the efficacy of this technique (four-quandrant sequential streak [FQS]) with two commonly used Campylobacter broths (Bolton's and Tecra) with whole...
Wed, 09/28/2016 - 01:03
Mithila Ferdous, Anna M. D. Kooistra-Smid, Kai Zhou, John W. A. Rossen, Alexander W. Friedrich
Wed, 09/28/2016 - 00:00
To the Editor: Cholera—a severe, waterborne, virulent enteric infection caused by toxigenic strains of Vibrio cholera—frequently causes epidemics in developing countries and sporadic cases or local outbreaks in developed countries. The geographic features of Russia and intensive globalization have established favorable conditions for travel-associated cholera from regions to which it is endemic....
Wed, 09/28/2016 - 00:00
Exposure to mycotoxin-contaminated feeds represents a serious health risk. This has necessitated the need for the establishment of practical methods for mycotoxin decontamination. This study investigated the effects of citric acid (CA) and lactic acid (LA) on common trichothecene mycotoxins in feeds contaminated with Fusarium mycotoxins. Contaminated feed samples were processed either with 5% CA...
Tue, 09/27/2016 - 17:00
Madushini N. Dharmasena, Catherine M. Feuille, Carly Elizabeth C. Starke, Arvind A. Bhagwat, Scott Stibitz, Dennis J. Kopecko The licensed oral, live-attenuated bacterial vaccine for typhoid fever, Salmonella enterica serovar Typhi strain Ty21a, has also been utilized as a vaccine delivery platform for expression of diverse foreign antigens that stimulate protection against shigellosis, anthrax...
Tue, 09/27/2016 - 03:55
Abstract BACKGROUNDThe recent finding of acrylamide (AA), which is formed in cooked foods, has raised human health concerns. AA is metabolized by CYP2E1 to glycidamide (GA). GA forms DNA adducts. In this study, we examined the inhibitory effects of wasabi (Japanese horseradish, Wasabia japonica) roots and leaves as well as their active component, allyl isothiocyanate (AIT), on the formation and...
Tue, 09/27/2016 - 00:34
An α-l-arabinofuranosidase (Abf) encoding gene was obtained via genomic mining from a Ruminococcus albus strain. The specific activity of this GH 51 Abf was 73.3 U/mg at pH 6.0 and 50 °C. The modification of Abf, aimed at improving thermostability, was performed through different strategies. Structure-based rational design using the PoPMuSiC and the Enzyme Thermal Stability System (ETSS)...
Mon, 09/26/2016 - 17:00
Andrea R. Ottesen, Sasha Gorham, Elizabeth Reed, Michael J. Newell, Padmini Ramachandran, Travis Canida, Marc Allard, Peter Evans, Eric Brown, James Robert White An important data gap in our understanding of the phyllosphere surrounds the origin of the many microbes described as phyllosphere communities. Most sampling in phyllosphere research has focused on the collection of microbiota without...
Mon, 09/26/2016 - 17:00
Yussaira Castillo, Jin Suzuki, Kenta Watanabe, Takashi Shimizu, Masahisa Watarai Insect infection models have been used increasingly to study various pathogenic agents in evaluations of pathogenicity and drug efficacy. In this study, we demonstrated that larvae of the silkworm Bombyx mori are useful for studying Listeria monocytogenes infections in insects. Infection with the L. monocytogenes...
Mon, 09/26/2016 - 11:14
The riboflavin analogs roseoflavin (RoF) and 8-demethyl-8-aminoriboflavin (AF) are produced by the bacteria Streptomyces davawensis and Streptomyces cinnabarinus. Riboflavin analogs have the potential to be used as broad-spectrum antibiotics and we therefore studied the metabolism of riboflavin (vitamin B2), RoF and AF in the human pathogen Listeria monocytogenes, a bacterium which is a...
Sat, 09/24/2016 - 18:03
Publication date: 5 December 2016 , Volume 238 Author(s): Denis Butscher, Hanne Van Loon, Alexandra Waskow, Philipp Rudolf von Rohr, Markus Schuppler Fresh produce is frequently contaminated by microorganisms, which may lead to spoilage or even pose a threat to human health. In particular sprouts are considered to be among the most risky foods sold at retail since they are grown in...
Sat, 09/24/2016 - 00:00
Globally, the trichothecene mycotoxins deoxynivalenol (DON) and nivalenol (NIV) are among the most widely distributed mycotoxins that contaminate small grain cereals. In this study, a bacterial consortium, PGC-3, with de-epoxydation activity was isolated from soil by an in situ soil enrichment method. Screening of 14 soil samples that were sprayed with DON revealed that 4 samples were able to...
Sat, 09/24/2016 - 00:00
This study was conducted to investigate the toxic effects of aflatoxin B1 (AFB1) and evaluate the effects of sporoderm-broken spores of Ganoderma lucidum (SSGL) in relieving aflatoxicosis in broilers. A total of 300 one-day-old male Arbor Acre broiler chickens were randomly divided into four dietary treatments; the treatment diets were: Control (a basal diet containing normal peanut meal); AFB1 (...
Sat, 09/24/2016 - 00:00
In China, consumers sometimes get special eggs, which are characterized by the pasty, firm, and elastic texture of yolk from hard-boiled eggs and are usually called “rubber eggs” by Chinese customers. Consumers do not like the texture of rubber eggs and often associate the odd texture of the yolk to food safety risks. The objective of this study was to assess the physical and chemical...
Fri, 09/23/2016 - 18:03
Publication date: 5 December 2016 , Volume 238 Author(s): Shin Young Park, Sujin Kang, Sang-Do Ha In Korea, edible seaweeds are potentially regarded as high-risk foods with respect to enteric norovirus (NoV) and non-pathogenic generic Escherichia coli. This study investigated the antimicrobial effects of 5%, 10%, and 15% vinegar (6% acetic acid) on the survival of murine norovirus-1 (...
Fri, 09/23/2016 - 12:01
Giardia lamblia is a pathogen transmitted by water and food that causes infection worldwide. Giardia genotypes are classified into 8 assemblages (A–H). Assemblages A and B are detected in humans, but they are potentially zoonotic because they infect other mammalian hosts. Giardia in samples from 44 children was genotyped. Conserved fragments of the genes encoding β-giardin and...
Fri, 09/23/2016 - 11:15
We examined clinical samples from Nigerian patients with acute watery diarrhea for Vibrio cholerae during the 2010 cholera outbreak. A total of 109 suspected isolates were characterized, but only 57 V. cholerae strains could be confirmed using multiplex real-time PCR as well as rpoB sequencing and typed as V. cholerae O:1 Ogawa biotype El Tor. This finding highlighted the need for accurate...
Fri, 09/23/2016 - 11:15
Norovirus full-genome sequencing is challenging due to sequence heterogeneity among genomes. Previous methods have relied on PCR amplification, which is problematic due to primer design, and transcriptome sequencing (RNA-Seq), which nonspecifically sequences all RNA, including host and bacterial RNA, in stool specimens. Target enrichment uses a panel of custom-designed 120-mer RNA baits that are...
Fri, 09/23/2016 - 01:59
Tetrodotoxin (TTX) is an emerging toxin in the European marine environment. It has various known structural analogues. It acts as a sodium channel blocker; the ability of each analogue to bind to the sodium channel varies with the particular structure of each analogue. Thus, each analogue will vary in its toxic potential. TTX analogues co-occur in food samples at variable concentrations. An LC-MS...
Fri, 09/23/2016 - 01:22
Hechao Du, Maoda Pang, Yuhao Dong, Yafeng Wu, Nannan Wang, Jin Liu, Furqan Awan, Chengping Lu, Yongjie Liu
Fri, 09/23/2016 - 01:22
Pauline Bondue, Sébastien Crèvecoeur, François Brose, Georges Daube, Marie-Christine Seghaye, Mansel W. Griffiths, Gisèle LaPointe, Véronique Delcenserie
Fri, 09/23/2016 - 01:17
Zearalenone (ZEN) and ochratoxin A (OTA) are secondary toxic metabolites of fungi that can contaminate a wide range of food and feedstuff. In this study, the effects of ozone treatment on ZEN and OTA and the quality of ozonised corn are investigated. Ozone significantly affects ZEN and OTA solutions. ZEN was undetectable 5 s after being treated with 10 mg l–1 ozone. However, OTA was resistant to...
Thu, 09/22/2016 - 15:20
Abstract Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in Tunisia. Bulk tank milk (n = 32) and individual cow's milk (n = 130)...
Thu, 09/22/2016 - 09:31
Abstract The inhibition capacity of four combinations of three lactic acid bacteria (LAB) (Lactococcus lactis, Lactobacillus plantarum and Carnobacterium piscicola) has been demonstrated against Listeria monocytogenes in fresh farmed sea bass fillets artificially contaminated. The four combinations gave promising results, having a significant bacteriostatic effect against all tested microflora,...
Thu, 09/22/2016 - 08:16
Abstract This work was aimed at determining the occurrence and diversity of Listeria monocytogenes in a traditional meat-processing facility to reveal persistent contamination. A total of 268 samples, including 196 environmental samples and 72 meat samples were collected during a four-year period, and 70 were found to be L. monocytogenes positive. Molecular serotyping of 77 isolates classified...
Thu, 09/22/2016 - 04:40
Camel meat production for human consumption and pet food manufacture accounts for a relatively small part of overall red meat production in Australia. Reliable statistical data for the Australian production and consumption of camel meat are not available; however, it is estimated that 300,000 feral camels roam within the desert of central Australia, with an annual usage of more than 3000 camels...
Thu, 09/22/2016 - 04:35
Blue-green algae are responsible for the production of different types of toxins which can be neurotoxic, hepatotoxic, cytotoxic and dermatotoxic and that can affect both aquatic and terrestrial life. Since its discovery the neurotoxin β-methylamino-L-alanine (BMAA) has been a cause for concern, being associated with the neurodegenerative disease amyotrophic lateral sclerosis/Parkinsonism–...
Thu, 09/22/2016 - 04:25
In order to address the issue of excessive intake of aluminium (Al) from Al-containing food additives in the Chinese diet, this study conducted a dietary exposure assessment of Al in the general population based on the national surveillance data of Al content in foods and national food consumption data. It was found that the mean dietary exposure of the whole Chinese population to Al from Al-...
Thu, 09/22/2016 - 02:55
Shigella flexneri is a Gram-negative pathogen that invades the colonic epithelium and causes millions of cases of watery diarrhea or bacillary dysentery predominately in children under the age of 5 years in developing countries. The effector Shigella enterotoxin 2 (ShET2), or OspD3, is encoded by the sen or ospD3 gene on the virulence plasmid. Previous literature has suggested that ospD3 is in an...
Thu, 09/22/2016 - 00:00
Deoxynivalenol (DON) is a widespread trichothecene mycotoxin that commonly contaminates cereal crops and has various toxic effects in animals and humans. DON primarily targets the gastrointestinal tract, the first barrier against ingested food contaminants. In this study, an isobaric tag for relative and absolute quantitation (iTRAQ)-based phosphoproteomic approach was employed to elucidate the...
Thu, 09/22/2016 - 00:00
Citrinin (CIT) and ochratoxin A (OTA) are important mycotoxins, which frequently co-contaminate foodstuff. In order to assess the toxicologic threat posed by the two mycotoxins separately or in combination, their biological effects were studied here using genomic transcription profiling and specific live cell gene expression reporters in yeast cells. Both CIT and OTA cause highly transient...
Thu, 09/22/2016 - 00:00
Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disorder caused by the consumption of seafood contaminated with okadaic acid (OA) and dinophysistoxins (DTXs). OA and DTXs are potent inhibitors of protein phosphatases 2A, 1B, and 2B, which may promote cancer in the human digestive system. Their expression in dinoflagellates is strongly affected by nutritional and environmental factors....
Wed, 09/21/2016 - 20:00
The emerging enteric pathogen Campylobacter concisus is associated with prolonged diarrhea and inflammatory bowel disease. Previous studies have shown that C. concisus strains are very genetically diverse. Nevert...
Wed, 09/21/2016 - 20:00
In summer 2013, an excess of paediatric cases of haemolytic uraemic syndrome (HUS) in a southern region of Italy prompted the investigation of a community-wide outbreak of Shiga toxin 2-producing Escherichia coli (STEC) O26:H11 infections. Case finding was based on testing patients with HUS or bloody diarrhoea for STEC infection by microbiological and serological methods. A case-control study...
Wed, 09/21/2016 - 17:54
Publication date: 5 December 2016 , Volume 238 Author(s): Claire Zoellner, Fabiola Venegas, John J. Churey, Jorge Dávila-Aviña, Yrjo T. Grohn, Santos García, Norma Heredia, Randy W. Worobo Quality and safety of fresh produce are important to public health and maintaining commerce between Mexico and USA. While preventive practices can reduce risks of contamination and are...
Wed, 09/21/2016 - 07:02
A rapid liquid chromatographic-tandem mass spectrometric method was developed for the simultaneous determination of four natural and synthetic hormone residues (progesterone, testosterone, trenbolone acetate and zeranol) in animal tissue samples. Sample preparation was optimised to minimise time and solvent consumption. Meat samples were mechanically homogenised and digested in a procedure that...
Wed, 09/21/2016 - 06:39
Sulphites are a family of additives regulated for use worldwide in food products. They must be declared on the label if they are present in concentrations greater than 10 mg kg–1, determined as sulphur dioxide (SO2). The current US regulatory method for sulphites, the optimised Monier–Williams method (OMW), produces false-positive results with vegetables from the Allium (garlic) and Brassica (...
Wed, 09/21/2016 - 06:39
Sulphur dioxide (SO2) is an important preservative for wine, but its presence in foods can cause allergies and this has given impetus to the research for alternatives. The aim of this study was to reduce levels of sulfite in wine production using mixtures with lysozyme and dimethyl dicarbonate and examine the influence on levels of volatile and biogenic amines. To do so, vinifications were...
Wed, 09/21/2016 - 01:00
Vengadesh Letchumanan, Hooi-Leng Ser, Kok-Gan Chan, Bey-Hing Goh, Learn-Han Lee
Wed, 09/21/2016 - 01:00
Davide Gottardi, Danka Bukvicki, Sahdeo Prasad, Amit K. Tyagi
Wed, 09/21/2016 - 01:00
Zhongjing Lu, Christopher R. Dockery, Michael Crosby, Katherine Chavarria, Brett Patterson, Matthew Giedd
Tue, 09/20/2016 - 21:20
Abstract AimsThis study was conducted to evaluate the biocontrol potential of Bacillus subtilis CICC 10034, Rhodobacter sphaeroides CGMCC 1.2182 and Agrobacterium tumefaciens CGMCC 1.2554 against patulin (PAT)-producer Penicillium expansum and their ability to remove PAT. Methods and ResultsBacillus subtilis effectively inhibited P. expansum both on apples and in in vitro experiments, which...

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