Research Publications from Peer-Reviewed Journals

Fri, 11/29/2019 - 03:18
Author(s): Limei Chen, Yanfen Liao, Xiaoqian Ma, Yadong NiuAbstractIncineration of sludge in waste incinerators is a trend of sludge disposal, and the problem of heavy metal in horizontal flue ash is a concern. Horizontal flue ash in waste incinerator was collected before and after the sludge was co-combusted. XRF, SEM-DES, and ICP were used to characterize the effect of sludge on the...
Fri, 11/29/2019 - 03:18
Author(s): Francesco Chidichimo, Michele De Biase, Salvatore StrafaceAbstractGroundwater is the environmental compartment very often involved in the broader issues related to contaminated sites characterization and reclamation. It is not uncommon to find areas in which some substances directly linked to the petrographic composition of the aquifer reach high values exceeding the limits set by the...
Fri, 11/29/2019 - 03:18
Author(s): Kuan-Hung Lu, Yi-Jyun Sheen, Tsui-Ping Huang, Shu-Hui Kao, Chun-Lung Cheng, Cheng-An Hwang, Shiowshuh Sheen, Lihan Huang, Lee-Yan SheenAbstractCooked rice with pork floss (CRPF) wrapped in dried seaweed is one of the most popular ready-to-eat (RTE) foods in many Asian countries, particularly in Taiwan. The products are susceptible to Staphylococcus aureus contamination and temperature...
Fri, 11/29/2019 - 03:17
Author(s): SeEun Choe, Sok Song, Gyu-Nam Park, Jihye Shin, Ki-Sun Kim, Ra MiCha, Bang-Hun Hyun, Bong-Kyun Park, Dong-Jun AnABSTRACTThe hepatitis E virus (HEV) is known to have 4 genotypes but only one serotype. Genotype 1 and 2 infect humans only and genotype 3 and 4 infect humans, pigs and other animal species. Pig and wild boar are also known as reservoirs of HEV infection. Of the 2,736 wild...
Fri, 11/29/2019 - 03:17
Author(s): E.L. Shebs, M.J. Lukov, F.M. Giotto, E.S. Torres, A.S. de MelloAbstractAfter High Event Periods, beef subprimals are usually removed from vacuum and treated with antimicrobials. After re-packaging, subprimals are tested to verify the presence of STEC. In this study, bacteriophage and organic acids were applied on beef contaminated with STEC O157:H7 to evaluate the efficiency of...
Fri, 11/29/2019 - 03:16
Author(s): Tao Lei, Fufeng Jiang, Min He, Jumei Zhang, Haiyan Zeng, Moutong Chen, Rui Pang, Shi Wu, Lei Wei, Juan Wang, Yu Ding, Qingping WuAbstractVibrio parahaemolyticus is the leading cause of foodborne bacterial poisoning in China. The aim of this research is to conduct a study on the prevalence, virulence, and antimicrobial resistance of V. parahaemolyticus from different types of food...
Fri, 11/29/2019 - 03:16
Author(s): Yang Long, Xin Liu, Xiao-zhen Tan, Chun-xia Jiang, Shao-wei Chen, Guan-nan Liang, Xue-mei He, Jian Wu, Tao Chen, Yong XuAbstractGrowing epidemiological evidence has shown that exposure to polychlorinated biphenyls (PCBs) is harmful to the cardiovascular system. However, how PCB 118-induced oxidative stress mediates endothelial dysfunction is not fully understood. Here, we explored...
Fri, 11/29/2019 - 03:16
Author(s): Elisa Costa, Chiara Gambardella, Veronica Piazza, Massimo Vassalli, Francesca Sbrana, Silvia Lavorano, Francesca Garaventa, Marco FaimaliAbstractFor the first time, we report a correspondence between microplastics (MP) ingestion and ecotoxicological effects in gelatinous zooplankton (Cnidarian jellyfish). The ephyra stage of the jellyfish Aurelia sp. was exposed to both environmental...
Fri, 11/29/2019 - 03:16
Author(s): Wei Gao, Misako Yamada, Rieko Ohki, Yuji Nagashima, Ryohei Tatsuno, Koichi Ikeda, Kentaro Kawatsu, Tomohiro Takatani, Osamu ArakawaAbstractThe tetrodotoxin (TTX) uptake ability of pufferfish Takifugu rubripes tissues and its growth-associated changes were investigated using an in vitro tissue slice incubation method. Tissue slices prepared from the liver, skin, and intestine of a non-...
Fri, 11/29/2019 - 03:15
Author(s): Loïc Durand, Stéphanie La Carbona, Alain Geffard, Alessia Possenti, Jitender P. Dubey, Marco LalleAbstractThe apicomplexan parasite Toxoplasma gondii can infect humans and cause toxoplasmosis. T. gondii has been highly prioritized among the foodborne parasites regarding its global impact on public health. Human infection can occur through multiple routes, including the ingestion of raw...
Fri, 11/29/2019 - 03:15
The objective of this study is to determine the occurrence and level of aflatoxins (AFs) contamination in freshly harvested maize for human consumption in rural Kenya. Maize kernels and freshly milled maize flour (n = 338) were collected from households in Siaya and Makueni counties. While both counties are representatives of different environmental and climate conditions, Makueni County is the...
Fri, 11/29/2019 - 03:13
In this study, we determined the concentrations of polycyclic aromatic hydrocarbons (PAHs) in road dust from Myanmar, Japan, Taiwan, and Vietnam. PAHs were detected in urban and rural areas of Myanmar at mean concentrations of 630 ng/g dry weight and 200 ng/g dry weight, respectively. PAHs were also detected in road dust from Vietnam (mean 1700 ng/g) and Taiwan (2400 ng/g). PAH diagnostic ratios...
Fri, 11/29/2019 - 03:12
Being natural plant antimicrobials, saponins have potential for use as biopesticides. Nevertheless, their activity in plant–pathogen interaction is poorly understood. We performed a comparative study of saponins' antifungal activities on important crop pathogens based on their effective dose (EC50) values. Among those saponins tested, aescin showed itself to be the strongest antifungal agent. The...
Fri, 11/29/2019 - 03:12
Antimicrobial resistance against enteroaggregative Escherichia coli (EAEC), an emerging food-borne pathogen, has been observed in an increasing trend recently. In the recent wake of antimicrobial resistance, alternate strategies especially, cationic antimicrobial peptides (AMPs) have attracted considerable attention to source antimicrobial technology solutions. This study evaluated the in vitro...
Fri, 11/29/2019 - 03:12
Microbial contamination of beef cattle carcases and subsequent cross-contamination during processing is inevitable and virtually impossible to prevent. The understanding of microbial contamination in the beef industry is currently limited to hypotheses based on traditional microbiological tools. Additionally, the complex structural and functional responses of beef cattle microbial communities to...
Fri, 11/29/2019 - 03:12
Membrane blebs are released from Gram-negative bacteria, however, little is known about Brucella blebs. This work pursued two objectives, the first was to determine and identify the proteins in the membrane blebs by proteomics and in silico analysis. The second aim was to evaluate the use of membrane blebs of Brucella abortus 2308 and B. abortus RB51 as an acellular vaccine in vivo and in vitro....
Fri, 11/29/2019 - 03:12
Stenotrophomonas maltophilia is an emerging opportunistic pathogen that, on the one hand, causes severe nosocomial infection in immunocompromised populations with a high mortality rate and, on the other hand, is present ubiquitously in the environment. This study, for the first time to the best of our knowledge, isolated and characterized S. maltophilia from leafy green vegetables produced by...
Fri, 11/29/2019 - 03:12
Modern threats of bioterrorism force the need for multiple detection of biothreat agents to determine the presence or absence of such agents in suspicious samples. Here, we present a rapid electrochemical fiveplex biochip screening assay for detection of the bioterrorism relevant low molecular weight toxins saxitoxin, microcystin-LR, T-2 toxin, roridin A and aflatoxin B1 relying on anti-idiotypic...
Fri, 11/29/2019 - 03:12
Salmonella is one of the most common food-borne pathogens. It can be transmitted between chickens, as well as to people by contaminated poultry products. In our study, we distinguished chickens with different resistances mainly based on bacterial loads. We compared the cecal tonsil transcriptomes between the susceptible and resistant chickens after Salmonella infection, aiming to identify the...
Thu, 11/28/2019 - 03:43
by Seinen Chow, Nobuharu Inaba, Satoshi Nagai, Hiroaki Kurogi, Yoji Nakamura, Takashi Yanagimoto, Hideki Tanaka, Daisuke Hasegawa, Taiga Asakura, Jun Kikuchi, Tsutomu Tomoda, Taketoshi Kodama Natural diets of leptocephalus larvae have been enigmatic. In this study, we collected DNA samples from the gut contents and body surface of leptocephali belonging to the five Anguilliform families (...
Thu, 11/28/2019 - 03:43
by Felipe Betoni Saraiva, Ana Caroline Cavalcante de Araújo, Anna Érika Vieira de Araújo, José Procópio Moreno Senna Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium responsible for serious nosocomial and community-acquired infections worldwide. Since few antibiotics are effective for treating MRSA infections, the development of new therapies is of great...
Thu, 11/28/2019 - 03:43
ABSTRACT Vibrio cholerae is a noninvasive pathogen that colonizes the small intestine and produces cholera toxin, causing severe secretory diarrhea. Cholera results in long lasting immunity, and recent studies have improved our understanding of the antigenic repertoire of V. cholerae. Interactions between the host, V. cholerae, and the intestinal microbiome are now recognized as factors which...
Thu, 11/28/2019 - 03:42
Single-nucleotide polymorphisms (SNPs) are widely used for whole-genome sequencing (WGS)-based subtyping of foodborne pathogens in outbreak and source tracking investigations. Mobile genetic elements (MGEs) are commonly present in bacterial genomes and may affect SNP subtyping results if their evolutionary history and dynamics differ from that of the bacterial chromosomes. Using Salmonella...
Thu, 11/28/2019 - 03:42
Escherichia coli is a leading contributor to infectious diarrhea and child mortality worldwide, but it remains unknown how alterations in the gut microbiome vary for distinct E. coli pathotype infections and whether these signatures can be used for diagnostic purposes. Further, the majority of enteric diarrheal infections are not diagnosed with respect to their etiological agent(s) due to...
Thu, 11/28/2019 - 03:42
Escherichia coli segregates into phylogenetic groups, with group B2 containing both extraintestinal pathogenic E. coli (ExPEC) and enteropathogenic E. coli (EPEC) strains. Ten main B2 subgroups (subgroups I to X)/sequence type complexes (STcs), as well as EPEC lineages, have been identified. In the current study, we characterized ExPEC and EPEC strains of E. coli B2 phylogenetic subgroups/STcs...
Thu, 11/28/2019 - 03:42
Campylobacter jejuni, a leading cause of gastroenteritis worldwide, has been frequently isolated from recreational rivers and streams in New Zealand, yet the public health significance of this is unknown. This study uses molecular tools to improve our understanding of the epidemiology and sources of Campylobacter in recreational waterways, with a view to preventing human infection....
Thu, 11/28/2019 - 03:40
Author(s): Ronghua Wu, Junyu Shen, Xinlian Lai, Tao He, Yun LiAbstractThe disease outbreak caused by viral infection and bacterial pathogens has hampered the sustainable development of the gibel carp (Carassius auratus gibelio) industry, lack of monoclonal antibodies against serum immunoglobulin (Ig) of gibel carp has impeded the development of nonfatal immunoassays in detection of pathogen...
Thu, 11/28/2019 - 03:37
Author(s): Cailin Liu, Chunguang Chen, Yafei Ye, Xiaogai Li, Jingjing Sun, Lijuan Xu, Liang MingAbstractTwo linezolid-resistant and teicoplanin-intermediate Staphylococcus epidermidis strains were isolated from blood cultures in China. The two S.epidermidis strains were methicillin-resistant and showed multidrug-resistance patterns, in addition, population analysis profiling/area under the curve...
Thu, 11/28/2019 - 03:37
Author(s): Clarissa Silveira Luiz Vaz, Daiane Voss-Rech, Luana Alves, Arlei Coldebella, Liana Brentano, Iara Maria TrevisolAbstractSalmonella Enteritidis remains a leading cause of human foodborne disease, mostly associated with the consumption of contaminated poultry products. To more strategically implement a phage therapy scheme for S. Enteritidis control in broilers, a cocktail containing...
Thu, 11/28/2019 - 03:37
Author(s): Antonise M. Jaguezeski, Aleksandro S. da Silva, Teane M.A. Gomes, Nathieli B. Bottari, Thalisson F. Lopes, Renan A. Cechin, Vera M. Morsch, Maria R.C. Schetinger, Janice L. Giongo, Rodrigo de A. VaucherAbstractThe cholinergic, purinergic and oxidative stress systems were related to nervous system damage in some pathologies, as well as being involved in pro-inflammatory and anti-...
Thu, 11/28/2019 - 03:36
Author(s): Salma Ouhibi, Arnau Vidal, Carla Martins, Ridha Gali, Abderrazzek Hedhili, Sarah De Saeger, Marthe De BoevreAbstractBiomarker-driven research has been proposed as a successful method to assess the exposure of individuals to xenobiotics, including mycotoxins, through estimation of their metabolites in biological fluids. A methodology to determine patulin (PAT) and citrinin (CIT) in...
Thu, 11/28/2019 - 03:36
Author(s): Manuela Zadravec, Nada Vahčić, Dragan Brnić, Ksenija Markov, Jadranka Frece, Relja Beck, Tina Lešić, Jelka PleadinAbstractXerophilic species of Aspergillus, Penicillium and Eurotium genera from surfaces of dry-cured traditional meat products (TMPs) can cause mycotoxin contamination during uncontrolled household processing. The aim of this study was to investigate into surface moulds...
Thu, 11/28/2019 - 03:36
Author(s): Shogo Yamaki, Soya Kuronuma, Yuji Kawai, Koji YamazakiAbstractHistamine (scombroid) poisoning is a foodborne illness caused by ingestion of histamine-contaminated seafood; therefore, inhibition of the growth of histamine-producing bacteria is key for it prevention. Infection of pathogenic bacteria by bacteriophages (phages) is being developed to prevent multiple foodborne illnesses....
Thu, 11/28/2019 - 03:35
Author(s): Kehan Fu, Ling Wang, Cuiyun Wei, Jie Li, Jie Zhang, Zhen Zhou, Yong LiangAbstractArtificial sweeteners (ASs) are applied widely as sucrose substitutes in food, pharmaceuticals, and personal-care products, which results in their release into surface water. The occurrence of ASs in aquatic environments in China has rarely been reported. In this study, we determined the concentration of...
Thu, 11/28/2019 - 03:35
Author(s): Lili Yang, Fen Jin, Guorui Liu, Yang Xu, Minghui Zheng, Cui Li, Yuanping YangAbstractWe collected surface sediments from the Chaobai river, the dominant source of drinking water for Beijing, China, to assess the status of contamination by polychlorinated biphenyls and evaluate their sources. Total concentrations of polychlorinated biphenyls in the sediments ranged from 0.125 to 70.6 ng...
Thu, 11/28/2019 - 03:32
Since 2004, there have been multiple outbreaks of H5 highly pathogenic avian influenza (HPAI) viruses in Laos. Here, we isolated H5N1 HPAI viruses from poultry outbreaks in Laos during 2015–2018 and investigated their genetic characteristics and pathogenicity in chickens. Phylogenetic analysis revealed that the isolates belonged to clade 2.3.2.1c, and that they differed from previous Laos viruses...
Thu, 11/28/2019 - 03:32
In July 2016, an avian influenza outbreak in duck farms in Yogyakarta province was reported to Disease Investigation Center (DIC) Wates, Indonesia with approximately 1,000 ducks death or culled. In this study, two avian influenza (AI) virus subtypes, A/duck/Bantul/04161291‐OR/2016 (H5N1) and A/duck/Bantul/04161291‐OP/2016 (H9N2) isolated from ducks in the same farm during an AI outbreak in Bantul...
Thu, 11/28/2019 - 03:28
Since the introduction of allergen immunotherapy (AIT) over 100 years ago, focus has been on standardization of allergen extracts, with reliable molecular composition of allergens receiving the highest attention. While adjuvants play a major role in European AIT, they have been less well studied. In this Position Paper we summarize current unmet needs of adjuvants in AIT citing current evidence....
Thu, 11/28/2019 - 03:28
Staphylococcus aureus is able to grow in the presence of high concentrations of NaCl but the exact genetic factors contributing to this are unknown. Using a high‐throughput TN‐seq approach, we identified gene 957 as an important factor for the salt stress resistance in S. aureus. A 957‐mutant was not only salt sensitive but also showed increased peptidoglycan crosslinking, altogether highlighting...
Thu, 11/28/2019 - 03:28
LuxR‐type transcriptional factors are essential in many bacterial physiological processes. However, there have been no reports on their roles in Aeromonas hydrophila. In this study, six stable silent strains were constructed using shRNA. Significant decreases in the expression levels of luxR05, luxR08, luxR19, luxR11, luxR164 and luxR165 were shown in their respective strains by qRT‐PCR. The...
Thu, 11/28/2019 - 03:26
The presence of fungi and mycotoxins in silage (fermented maize) for cattle and other ruminants have been studied extensively compared to wrapped haylage (fermented grass) for horses and other monogastric animals. The purpose of this work was to examine the fungal diversity of wrapped haylage and conventional hay and to analyse the forage sample for fungal metabolites. Faeces samples were also...
Thu, 11/28/2019 - 03:26
UV-C treatment technology is proposed as a potential alternative to thermal treatments for decontamination of beverages because heat may adversely affect the nutritive value and quality. Effects of UV-C irradiation processing on microbial inactivation, ascorbic acid, and volatile aromatics of watermelon beverage were evaluated using a flow-through UV system. Selected microbial agents Escherichia...
Thu, 11/28/2019 - 03:25
Prostate is sensitive to endocrine hormone level, and the synergetic effect of estrogen and androgen is critical in prostate growth. The change of signal pathways caused by the imbalance of estrogen and androgen might function in the occurrence of prostate diseases. As a well-known endocrine disruptor compound, bisphenol A (BPA) can disturb the normal function of endocrine hormone and affect...
Thu, 11/28/2019 - 03:24
GCN2 (general control nonrepressed 2) is a serine/threonine-protein kinase that regulates translation in response to stressors such as amino acid and purin deprivation, cold shock, wounding, cadmium, and UV-C exposure. Activated GCN2 phosphorylates the α-subunit of the eukaryotic initiation factor 2 (eIF2) leading to a drastic inhibition of protein synthesis and shifting translation to specific...
Thu, 11/28/2019 - 03:24
Aspergillus ochraceus is reported to be the major contributor of ochratoxin A (OTA), classified as one of the possible human carcinogen (group 2B) by the International Agency for Research on Cancer. The heterotrimeric velvet complex proteins, LaeA/VeA/VelB, have been most studied in fungi to clarify the relation between light-dependent morphology and secondary metabolism. To explore possible...
Thu, 11/28/2019 - 03:24
Strategies to reduce economic losses associated with post-weaning diarrhea in pig farming include high-level dietary zinc oxide supplementation. However, excessive usage of zinc oxide in the pig production sector was found to be associated with accumulation of multidrug resistant bacteria in these animals, presenting an environmental burden through contaminated manure. Here we report on zinc...
Thu, 11/28/2019 - 03:24
In The Lancet Infectious Diseases, Michael R Wiley and colleagues1 report 23 near-complete genome sequences of Lassa virus from Liberia.1 Lassa virus is a member of the Mammarenavirus genus and is the causative agent of Lassa fever, a febrile disease that can be associated with high morbidity and mortality. Lassa virus is maintained in nature as a chronic infection of its natural reservoir...
Thu, 11/28/2019 - 03:24
In The Lancet Infectious Diseases, the GBD 2017 Non-Typhoidal Salmonella Invasive Disease Collaborators1 estimate the global burden of invasive non-typhoidal salmonella disease using Bayesian meta-regression tools. The results show a high but decreasing number of cases since 2005 with a continuously high case fatality rate, consistent with previous estimates. These findings show that serotypes of...
Thu, 11/28/2019 - 03:24
Enterotoxigenic Escherichia coli (ETEC) is one of the leading bacterial causes of intestinal inflammation and diarrhea. However, the ETEC is frequently resistant to common antibiotics. In this study, we explored ...
Wed, 11/27/2019 - 03:26
by Ghadir Fakhri Aljayyousi, Manar E. Abdel-Rahman, Asmaa El- Heneidy, Rana Kurdi, Eman Faisal Background Antimicrobial resistance (AMR) is a serious public health problem and a global concern. The inappropriate use of antibiotics has been identified by the World Health Organization as a major risk factor for AMR. Methods The purpose of this research study is to assess the prevalence of...

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