Research Publications (Food Safety)

Journal of Applied Microbiology, Accepted Article. Aims
To determine the occurrence, diversity, antibiotic resistance and biofilm formation of V. parahaemolyticus isolated from marine fishes in Bangladesh.

The role of livestock animals as a putative source of ESBL/pAmpC E. coli for humans is a central issue of research. In a large-scale pan-European surveillance, 2,993 commensal Escherichia spp. isolates were recovered from randomly collected fecal samples of healthy cattle, pigs and chickens in various abattoirs. One-hundred Escherichia spp. isolates (0.5% from cattle, 1.3% pigs, 8.0% chickens) fulfilled the criteria for cefotaxime and ceftazidime non-wildtype (EUCAST).

A comparative whole genome analysis was performed on three newly sequenced Escherichia coli O157:H7 strains with different stx profiles, previously isolated from feedlot cattle [C1-010 (stx1−, stx2c+), C1-057 (stx−), and C1-067 (stx1+, stx2a+)], as well as five foodborne outbreak strains and six stx-negative strains from NCBI. Phylogenomic analysis demonstrated that the stx2c-carrying C1-010 and stx-negative C1-057 strains were grouped with the six NCBI stx-negative E.

This study aimed to investigate the impact of the food matrix (orange juice and yogurt) on the effects of the spore-forming probiotic microorganism Bacillus coagulans GBI-30 6086 in health parameters and gastrointestinal tract (gut) bacterial diversity in Wistar male rats. Rats (n=48) were randomly distributed into six groups.

In Vibrio parahaemolyticus, type III secretion system 1 (T3SS1) is a major virulence factor that delivers effectors into the host eukaryotic cytoplasm; however, studies on its infection mechanism are currently limited. To determine the function of the vscF gene, we constructed the vscF deletion mutant ΔvscF and complementation strain CΔvscF.

ABSTRACT The human pulmonary environment is complex, containing a matrix of cells, including fibroblasts, epithelial cells, interstitial macrophages, alveolar macrophages and neutrophils. When confronted with foreign material or invading pathogens, these cells mount a robust response. Nevertheless, many bacterial pathogens with an intracellular lifecycle stage exploit this environment for replication and survival.

We report the complete genome sequence of selenate [Se(VI)]-reducing Shigella sonnei SE6-1, which was isolated from stream sediment from an industrial complex in Jeonju, South Korea. The genome sequence is 4,762,774 bp long, with a G+C content of 50.7% and 4,548 genes, including 4,440 coding sequences, 22 rRNA genes, and 86 tRNA genes.

by Joshua A. F. Sutton, Oliver T. Carnell, Lucia Lafage, Joe Gray, Jacob Biboy, Josie F. Gibson, Eric J. G. Pollitt, Simone C. Tazoll, William Turnbull, Natalia H. Hajdamowicz, Bartłomiej Salamaga, Grace R. Pidwill, Alison M. Condliffe, Stephen A. Renshaw, Waldemar Vollmer, Simon J. Foster

Author(s): Zhanwen Zheng, Yawen Xie, Sheng Ma, Junhong Tu, Jiahui Li, Sen Liang, Yunfeng Xu, Chao Shi

Author(s): R. Paranthaman, J.A. Moses, C. Anandharamakrishnan

Author(s): Paulo Vale, Inês Ribeiro, Susana M. Rodrigues

Author(s): Xiaoming Wang, Zhuoren Ling, Naiyan Sun, Yongshi Liu, Jinhu Huang, Liping Wang

Author(s): Mohammed S. Almuhayawi, Soad K. Al Jaouni, Saad M. Almuhayawi, Samy Selim, Mohamed Abdel-Mawgoud

Author(s): Xiang Li, Xiuhong Mao, Yujin Xu, Yan Li, Nan Zhao, Junxiu Yao, Yufeng Dong, Mulualem Tigabu, Xiyang Zhao, Shanwen Li

Author(s): Xiaoting Hu, Tao Li, Weihong Xu, Yourong Chai

Author(s): Kolawole I. Ayeni, Olusegun O. Atanda, Rudolf Krska, Chibundu N. Ezekiel

Author(s): E. Buscaroli, I. Braschi, C. Cirillo, A. Fargue-Lelièvre, G.C. Modarelli, G. Pennisi, I. Righini, K. Specht, F. Orsini

Author(s): Ting Du, Lunjie Huang, Jing Wang, Jing Sun, Wentao Zhang, Jianlong Wang

Background
Along with the intensification of global food supply, food security has become a fateful barrier throughout the global food trade and delivery. Reliable analytic techniques are in strong need to keep up with the continuous demand for food quality and safety monitoring.

Author(s): Xiuwen Qiu, Guixiang Zhou, Huijuan Wang, Xiaoyu Wu

An increasing number of studies have suggested that PAH contamination in dairy products demands high concern. This study established an efficient determination method for the European Union 15 + 1 PAHs and four PAH derivatives in dairy samples using a QuEChERS method coupled with GC-QqQ-MS. The optimized method obtained a recovery of 63.38–109.17% with a precision of 3.82–15.62%, and the limit of detection and limit of quantification were 0.08–0.78 and 0.27–2.59 μg/kg, respectively.

Aspergillus fungi are known to produce aflatoxins, among which aflatoxin B1 (AFB1) is the most potent carcinogen that is metabolised by cytochrome P450 (CYP450). In the liver, AFB1 is metabolised into exo-8,9-epoxide by the CYP1A2 enzymes. The resulting epoxide can react with guanine to cause DNA damage. Natural inhibitors are being identified. However, the modes of action are poorly understood.

The aim of the study was to determine whether the addition of vegetable biocholine (VB) in laying hens feed minimises the effects of daily intake of aflatoxin B1 (AFB1). We allocated Hy-line Brown line laying hens into four groups with four replications/group and four birds/repetition.

The current approaches remain insufficient for measuring chicken egg spoilage or present analytical limitations. This study aimed to complement the existing analyses and identify novel markers using liquid chromatography-high resolution mass spectrometry-based foodomics strategies. In the discovery set, comparative untargeted metabolomics was utilized to identify marker candidates in microbially inoculated chicken eggs.

Fusarium head blight (FHB) causes yield loss, quality reduction, and grain mycotoxin accumulations. A novel pydiflumetofen-containing fungicide, Miravis Ace, was recently registered in North America. The main objective of this study was to assess the efficacies of Miravis Ace and the timing of application alongside industry standard triazole fungicides (Prosaro, Caramba, Proline and Folicur) on suppressing FHB, reducing mycotoxins and improving wheat agronomic performance.