Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Sat, 01/10/2015 - 01:14
Singh, Randhir et al. The purpose of this study was to determine the gene expression of Escherichia coli O157:H7 heat shocked in dairy compost. A two-step real-time PCR assay was used to evaluate the expression of stress and virulence genes in E. coli O157:H7 heat shocked in compost at 47.5°C for 10 min. Heat-shocked E. coli O157:H7 in compost was isolated by using an immunomagnetic bead...
Sat, 01/10/2015 - 01:14
Peng, Shengfeng et al. Eugenol is a major phenolic component with diverse biological activities. However, it is difficult to formulate into an aqueous solution due to poor water solubility, and this limits its application. In the present study, eugenol nanoliposomes (EN) were prepared by combining the ethanol injection method with the dynamic high-pressure microfluidization method. Good...
Sat, 01/10/2015 - 01:14
Giacometti, Federica et al. Two quantitative risk assessment (RA) models were developed to describe the risk of salmonellosis and listeriosis linked to consumption of raw milk sold in vending machines in Italy. Exposure assessment considered the official microbiological records monitoring raw milk samples from vending machines performed by the regional veterinary authorities from 2008 to 2011,...
Sat, 01/10/2015 - 01:14
Differences in the geographic distribution of distinct trichothecene mycotoxins in wheat and barley were first recorded two decades ago. The different toxicological properties of deoxynivalenol (DON), nivalenol (NIV) and their acetylated derivatives require careful monitoring of the dynamics of these mycotoxins and their producers. The phylogenetic species concept has become a valuable tool to...
Sat, 01/10/2015 - 01:14
This paper deals with the migration of selected hydrocarbon contaminants, namely mineral oil hydrocarbons (MOH), diisopropyl naphthalenes (DIPN) and polyalphaolefins (PAO) from adhesives into dry semolina and egg pasta packaged in direct contact with recycled paperboard. Migration was monitored during its shelf life (for up to two years) simulating storage in a supermarket (packs on shelves) and...
Sat, 01/10/2015 - 01:14
The mycotoxin ochratoxin A (OTA) is known to be the main contaminant of cereal grain and has become increasingly important in recent years. Therefore, a survey of ochratoxigenic fungi and OTA contamination in China is a special challenge. This paper summarises data on cereals and moulds (Aspergillus niger, Penicillium verrucosum, Aspergillus ochraceus, for example) and on grain and OTA from 1973...
Sat, 01/10/2015 - 01:14
Aim To determine the presence of the T6SS in Campylobacter jejuni from diverse sources. Methods and Results The recently identified type VI secretion system (T6SS) is a bacterial injection machinery that plays a role in virulence, symbiosis, bacterial interactions and environmental stress responses. This system has been recently discovered in the major enteric pathogen Campylobacter jejuni. In...
Sat, 01/10/2015 - 01:14
Publication date: Available online 9 January 2015 Author(s): Hung King Tiong , Steven Hartson , Peter M. Muriana Extracts of surface proteins, with minimal artifacts from contaminating cytosolic components, are highly desirable for investigating surface factors involved in attachment and formation of biofilms by bacteria that are problematic in commercial food processing facilities. In...
Sat, 01/10/2015 - 01:14
Publication date: January 2015 , Volume 305, Issue 1 Author(s): Mashkoor Mohsin , Sebastian Guenther , Peter Schierack , Karsten Tedin , Lothar H. Wieler Due to increased release or production of Shiga toxin by Enterohemorrhagic Escherichia coli (EHEC) after exposure to antimicrobial agents, the role of antimicrobial agents in EHEC mediated infections remains controversial. Probiotics...
Sat, 01/10/2015 - 01:14
Azole fungicides have been reported to be the most effective active substances in the control of Fusarium Head Blight (FHB) and in the reduction of the main mycotoxins that occur in cereal grain, such as deoxynivalenol (DON). Four field experiments have been conducted in North West Italy, over a period of 2 growing seasons, in order to evaluate the effect of azole fungicide (prothioconazole)...
Fri, 01/09/2015 - 01:18
Of a total of 143 presumptive yeast isolates obtained from Galician (north-west Spain) short-ripened raw cow's milk starter-free cheeses, 27 exhibited microbial alcohol-conferred hemolytic (MACH) activity. Among the MACH-positive isolates, eight were identified as Yarrowia lipolytica and four as Kluyveromyces lactis. These isolates of potential technological interest were subjected to spot agar...
Fri, 01/09/2015 - 01:18
Chitosan beads were prepared by the method of inverse suspension cross-linking characterized and applied to remove patulin from aqueous solution. Batch adsorption experiments were performed to evaluate the optimum adsorption conditions and reusability. The results showed that chitosan beads were effective in removing patulin from aqueous solution. The maximum adsorption capacity of chitosan beads...
Fri, 01/09/2015 - 01:18
A novel procedure on vancomycin, α-glucosidase activity, cephalothin and sucrose for isolation of Cronobacter spp. from powdered infant formula (PIF) was developed. Meanwhile, a comparison between the new procedure, Druggan-Forsythe-Iversen (DFI) and modified lauryl sulfate tryptose (mLST) procedure was also carried out. The recovery rate is higher by the new procedure than those by DFI and mLST...
Fri, 01/09/2015 - 01:18
The glucose oxidase (GOD) produced by the fungus Aspergillus tubingensis will be used to improve the quality of bread. The GOD preparation was assessed in a set of toxicological tests to document its safety in use. The oral administration of the GOD preparation to rats at a dose level of 0.4 mg/kg body weight/day did not cause any overt toxic effect on the survival, food intake and body weight...
Fri, 01/09/2015 - 01:18
The accurate reliable detection and identification of microorganisms in food is critical to public safety. Consequently, it is extremely important to develop rapid and inexpensive methods for the detection of food microorganisms in order to minimize or even replace the traditional analysis methods that are expensive and time-consuming. In this study, the potential of mid-infrared spectroscopy was...
Fri, 01/09/2015 - 01:18
The aim of this study was to isolate lactic acid bacteria (LAB) from sucuk and to determine their antibiotic resistance and biogenic amine production abilities. A total of 65 presumptive LAB were isolated and they were molecularly identified as Pediococcus acidilactici (47.7%), Enterococcus faecium (36.9%), Lactobacillus sakei ssp. carnosus (4.6%), Lactobacillus sakei ssp. sakei (4.6%),...
Fri, 01/09/2015 - 01:18
Publication date: June 2015 , Volume 52 Author(s): Jelka Pleadin , Mladenka Malenica Staver , Nada Vahčić , Dragan Kovačević , Salvatore Milone , Lara Saftić , Giampiero Scortichini The aim of this study was to investigate the occurrence of aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) in different traditional meat products circulating on Croatian markets, produced by a large number of...
Fri, 01/09/2015 - 01:18
Publication date: June 2015 , Volume 52 Author(s): Ruchika Chauhan , Pratima R. Solanki , Jay Singh , Irani Mukherjee , T. Basu , B.D. Malhotra An electrochemical quartz crystal microbalance (EQCM) based label-free immunosensor has been developed for the quantitative detection of aflatoxin B1 (AFB1) in groundnut. The gold (Au) coated quartz crystal (6 MHz) functionalized with self-...
Fri, 01/09/2015 - 01:18
Publication date: June 2015 , Volume 52 Author(s): Kojiro Maeda , Yoichi Toyokawa , Naohiro Shimizu , Yuichiro Imanishi , Akikazu Sakudo Salmonella is one of the most important causative agents of food borne infections in terms of food hygiene. In this study, we investigated whether N 2 gas plasma, generated by applying a short high-voltage pulse to N 2 using a static induction...
Fri, 01/09/2015 - 01:18
Challenges to testing for the illicit use of anabolic substances in meat-producing animals stem from the production of new synthetic compounds and the administration of low-dose cocktails to circumvent detection by the surveillance schemes of European Union member states. This work evaluated for the first time GR-CALUX, a highly sensitive reporter gene assay, as a screening tool for the detection...
Fri, 01/09/2015 - 01:18
Shiitake mushrooms (Lentinula edodes) cultivated on bed-log are known to accumulate radiocaesium. Since the Fukushima–Diichi nuclear power plant accident (2011), the violation rate has been higher for log-cultivated shiitake than that for agricultural products or other foodstuffs. When testing shiitake mushrooms for radionuclide contamination, the validation of the sampling plan can be severely...
Fri, 01/09/2015 - 01:18
Foodborne Pathogens and Disease , Vol. 0, No. 0. Hepatitis E virus (HEV) infections occur in swine worldwide. The porcine infection is usually subclinical, but HEV genotypes 3 and 4 are zoonotic agents that cause sporadic, indigenous human cases of hepatitis E. The aims of this study were to investigate the occurrence and dynamics of HEV infections in young pigs by analyzing a total of 273 fecal...
Fri, 01/09/2015 - 01:18
Foodborne Pathogens and Disease , Vol. 0, No. 0. Based on recent risk assessments, up to 83% of listeriosis cases from deli meat in the United States are predicted to be from ready-to-eat deli meats contaminated during processing at retail grocery stores. Listeria monocytogenes is known to use sanitizer tolerance and biofilm formation to survive, but interplay of these mechanisms along with...
Fri, 01/09/2015 - 01:18
Publication date: June 2015 , Volume 48 Author(s): John C. Frelka , Linda J. Harris Changes in aerobic plate count (APC) and E scherichia coli /coliform count (ECC) of inshell walnuts and walnut kernels were evaluated during commercial harvest and postharvest handling. APC and ECC for inshell walnuts collected from the tree were 6 and 4 log CFU/nut, respectively; counts increased by 1 log...
Fri, 01/09/2015 - 01:18
Publication date: 16 March 2015 , Volume 197 Author(s): Tiago Casella , María Margarita Rodríguez , Juliana Tiemi Takahashi , Barbara Ghiglione , Milena Dropa , Ednei Assunção , Maurício Lacerda Nogueira , Nilton Lincopan , Gabriel Gutkind , Mara Corrêa Lelles Nogueira CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have been increasingly identified in...
Fri, 01/09/2015 - 01:18
Publication date: 16 March 2015 , Volume 197 Author(s): Kathryn A. Whitehead , Sebastien Olivier , Paul S. Benson , Nils Arneborg , Joanna Verran , Peter Kelly In the food industry microbial contamination of surfaces can result in product spoilage which may lead to potential health problems of the consumer. Surface properties can have a substantial effect on microbial retention. The...
Fri, 01/09/2015 - 01:18
James R. J. Haycocks, Prateek Sharma, Anne M. Stringer, Joseph T. Wade, David C. Grainger Enterotoxigenic Escherichia coli (ETEC) cause severe diarrhoea in humans and neonatal farm animals. Annually, 380,000 human deaths, and multi-million dollar losses in the farming industry, can be attributed to ETEC infections. Illness results from the action of enterotoxins, which disrupt signalling...
Fri, 01/09/2015 - 01:18
The presence of highly pathogen STEC in shellfish, upstream waters and sediment from coastal shellfish sites was evaluated using the ISO/TS-13136 method. Shellfish (oysters, mussels and cockles), water and sediment samples were collected monthly over a period of one year. The method used real-time PCR detection of stx1, stx2 and eae genes and genetic markers corresponding to the five major...
Thu, 01/08/2015 - 19:36
Diarrhoeagenic Escherichia coli (DEC) cause serious foodborne infections in humans. Total of 450 Shigatoxigenic E. coli (STEC) strains isolated from humans, animals and environment in Finland were examined by multiplex PCR targeting the virulence genes of various DEC pathogroups simultaneously. One per cent (3/291) of the human STEC and 14% (22/159) of the animal and environmental STEC had genes...
Thu, 01/08/2015 - 03:20
Vesicle fusion governs many important biological processes, and imbalances in the regulation of membrane fusion can lead to a variety of diseases such as diabetes and neurological disorders. Here we show that the Vibrio parahaemolyticus effector protein VopQ is a potent inhibitor of membrane fusion based on an in vitro...
Thu, 01/08/2015 - 03:20
The spatial spread of the highly pathogenic avian influenza virus H5N1 and its long-term persistence in Asia have resulted in avian influenza panzootics and enormous economic losses in the poultry sector. However, an understanding of the regional long-distance transmission and seasonal patterns of the virus is still lacking. In this...
Thu, 01/08/2015 - 01:13
Publication date: June 2015 , Volume 52 Author(s): Andoni Zuriarrain , Juan Zuriarrain , Ana Isabel Puertas , María Teresa Dueñas , Iñaki Berregi A quantitative determination method of lactic and acetic acids in cider is proposed by means of the proton nuclear magnetic resonance (1H NMR) technique. Lactic acid gives a doublet signal at 1.42 ppm in the spectrum and acetic acid a singlet...
Thu, 01/08/2015 - 01:13
Foodborne Pathogens and Disease , Vol. 0, No. 0. The United States Food and Drug Administration and the Department of Agriculture jointly published the “Guide to Minimize Microbial Food Safety Hazards for Fresh Fruits and Vegetables,” which is used as a basis for Good Agricultural Practices (GAP) audits. To understand barriers to incorporation of GAP by Minnesota vegetable farmers, a mail survey...
Thu, 01/08/2015 - 01:13
Publication date: June 2015 , Volume 48 Author(s): Lesley L. Duffy , Patrick J. Blackall , Rowland N. Cobbold , Narelle Fegan Poultry are considered a major source for campylobacteriosis in humans. A total of 1866 Campylobacter spp. isolates collected through the poultry processing chain were typed using flaA -restriction fragment length polymorphism to measure the impact of processing on...
Thu, 01/08/2015 - 01:13
Aims Organophosphorus pesticides are widely used in agriculture. Accordingly decontamination of these pesticides and their residual in environment is an important aim of researchers. One of the best approaches is enzymatic detoxification of these compounds with organophosphorus hydrolase (OPH). The immobilization of OPH on environmentally friendly supports is of great importance for developing...
Thu, 01/08/2015 - 01:13
Publication date: Available online 7 January 2015 Author(s): Julie-Hélène Fairbrother , Simon Dufour , John Morris Fairbrother , David Francoz , Éric Nadeau , Serge Messier Escherichia coli usually cause transient intramammary infections in dairy cows, but persistent intramammary infections have been observed. The objective of the study was to compare antimicrobial resistance and...
Thu, 01/08/2015 - 01:13
Background. Previous population-based estimates in the United States have shown a relatively high prevalence of hepatitis E virus (HEV) antibody. We sought to determine whether changes in the prevalence of HEV antibody have occurred over time. Methods. We analyzed data from the 2009–2010 National Health and Nutrition Examination Survey (NHANES) and NHANES III (1988–1994)....
Thu, 01/08/2015 - 01:13
The metal-organic framework ∞[(Nd0.9Eu0.1)2(DPA)3(H2O)3] was tested for extraction of thiamethoxam, thiacloprid, thiophanate-methyl, teflubenzuron, and bifenthrin from soursop exotic fruit (Annona muricata), with analysis using liquid chromatography with UV diode array detector (DAD). Experiments were carried out at one fortification level (0.5 mg kg−1, n = 5) and resulted in...
Thu, 01/08/2015 - 01:13
For simultaneously monitoring two classes of organophosphorus pesticide residues in food samples, a polyclonal antibody against a dual-generic hapten immunogen, which was prepared by conjugating two generic haptens (O,O-dimethyl thiophosphate hapten and O,O-diethyl phosphate hapten) to the same carrier protein BSA, was raised and used to develop a broad-specificity...
Thu, 01/08/2015 - 01:13
In the scope of this study, pesticide residue distribution within a fruit and the possible error deriving from sample preparation step were demonstrated with the analysis of benomyl residues in peel, pulp, and seeds of papaya fruits treated post-harvest. Benomyl residue, measured as carbendazim, in corresponding sections of peel and pulp of papaya fruits ranged from 0.178 to...
Wed, 01/07/2015 - 01:18
Foodborne Pathogens and Disease , Vol. 0, No. 0. Meat juice samples are used in serological assays to monitor infectious diseases within the food chain. However, evidence of inferior sensitivity, presumably due to low levels of antibodies in the meat juice compared to serum, has been presented, and it has been suggested that adjusting the dilution factor of meat juice in proportion to its blood...
Wed, 01/07/2015 - 01:18
Foodborne Pathogens and Disease , Vol. 0, No. 0. Weak organic acids are widely used as preservatives and disinfectants in the food industry. Despite their widespread use, the antimicrobial mode of action of organic acids is still not fully understood. This study investigated the effect of acetic acid on the cell membranes and cellular energy generation of four Salmonella strains. Using a nucleic...
Wed, 01/07/2015 - 01:18
Foodborne Pathogens and Disease , Vol. 0, No. 0. In the present study, 500 raw beef, pork, and chicken meat samples and 100 pooled egg samples were analyzed for the presence of vancomycin-resistant enterococci, vancomycin-resistance phenotypes, and resistance genes. Of 141 isolates of enterococci, 88 strains of Enterococcus faecium and 53 strains of E. faecalis were identified. The most prevalent...
Wed, 01/07/2015 - 01:18
Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens....
Wed, 01/07/2015 - 01:18
Publication date: June 2015 , Volume 48 Author(s): Tekla Engelhardt , Helena Albano , Gabriella Kiskó , Csilla Mohácsi-Farkas , Paula Teixeira Bacteriocin producing lactic acid bacteria (LAB) cultures can be used as biopreservatives in fermented food products; thus the food industry is interested in stable cultures that produce bacteriocins consistently. Inhibtion of Listeria spp. by...
Wed, 01/07/2015 - 01:18
The present study investigated the effect of sumacSWE) and barberry water extracts (BWE) on microbial growth and chemical changes in ground sheep meat during refrigerated storage. The meat samples were explored for changes in total viable count (TVC), lactic acid bacteria (LAB), coliforms, yeasts and Listeria monocytogenes populations as well as thiobarbituric acid reactive substances (TBARS)...
Tue, 01/06/2015 - 06:20
Publication date: January 2015 , Volume 45, Issue 1 Author(s): Steven C. Olsen , Steve A. Carlson Plazomicin is a next-generation aminoglycoside with a potentially unique set of clinical characteristics compared with other aminoglycosides. This study assessed the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of plazomicin against 15 clinical...
Tue, 01/06/2015 - 03:20
The aim of this study was to characterize 27 feed additives marketed as mycotoxin binders and to screen them for their in vitro zearalenone (ZEN) adsorption. Firstly, 27 mycotoxin binders, commercially available in Belgium and The Netherlands, were selected and characterized. Characterization was comprised of X-ray diffraction (XRD) profiling of the mineral content and d-spacing, determination...
Tue, 01/06/2015 - 03:20
Ian F. Connerton and Steven P.T. Hooton Campylobacter jejuni is a worldwide cause of human diarrhoeal disease. Clustered Repetitively Interspaced Palindromic Repeats (CRISPRs) and associated proteins allow Bacteria and Archaea to evade bacteriophage and plasmid infection. Type II CRISPR systems are found in association with combinations of genes encoding the CRISPR-associated Cas1, Cas2, Cas4 or...
Tue, 01/06/2015 - 01:15
Publication date: June 2015 , Volume 52 Author(s): Omaima M. Ahmed , Philipus Pangloli , Cheng-An Hwang , Svetlana Zivanovic , Tao Wu , Doris D'Souza , F.Ann Draughon Listeria monocytogenes is a psychrotrophic foodborne pathogen that has been isolated from ready-to-eat meat and poultry products (RTE meats). The purpose of this study was to quantify lactate and acetate levels in retail...

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